Comment[GEAAccession]	E-GEAD-884
MAGE-TAB Version	1.1
Investigation Title	RNA-seq of mouse iWAT-derived primary cells
Experiment Description	The experiment is designed to elucidate the molecular mechanisms underlying the age-related decline in beige adipocyte induction.
Experimental Design	cell type comparison design
Experimental Factor Name	temperature
Experimental Factor Type	temperature
Person Last Name	Horino	Ikeda
Person First Name	Masato	Kenji
Person Affiliation	Institute of Science Tokyo	
Person Roles	submitter	submitter
Public Release Date	2025-11-22
Protocol Name	P-GEAD-3411	P-GEAD-3412	P-GEAD-3413	P-GEAD-3414	P-GEAD-3415
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	iWAT was dissociated into cells and FACS sorting was performed with the usage of antibodies.	Total RNA was extracted by using miRNeasy micro kit.	NEBNext rRNA depletion kit and NEBNext ultra II directional RNA library prep for IlluminaNext was used.	NextSeq500EB was used.	TPM was performed.
SDRF File	E-GEAD-884.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB19629
Comment[Last Update Date]	2025-11-22