Comment[GEAAccession]	E-GEAD-886
MAGE-TAB Version	1.1
Investigation Title	RNA-seq of Car4 knocked-down cells
Experiment Description	The experiment is designed to elucidate the molecular mechanisms underlying the age-related decline in beige adipocyte induction.
Experimental Design	cell type comparison design
Experimental Factor Name	shRNA
Experimental Factor Type	shRNA
Person Last Name	Horino	Ikeda
Person First Name	Masato	Kenji
Person Affiliation	Department of Molecular Endocrinology and Metabolism, Institute of Science Tokyo	
Person Roles	submitter	submitter
Public Release Date	2025-12-03
Protocol Name	P-GEAD-3421	P-GEAD-3422	P-GEAD-3423	P-GEAD-3424	P-GEAD-3425
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Immortalized preadipocytes were incubated with lentiviral shRNA and antibiotic selection was performed.	Total RNA was extracted using RNeasy mini kit.	TruSeq stranded mRNA library prep kit was used for library construction.	NovaSeq6000 was used.	Raw reads were converted to TPM.
SDRF File	E-GEAD-886.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB19692
Comment[Last Update Date]	2025-12-03