Comment[GEAAccession]	E-GEAD-888
MAGE-TAB Version	1.1
Investigation Title	Analysis data for snRNAseq of inguinal white adipose tissues and liver
Experiment Description	Analysis data for snRNAseq of inguinal white adipose tissues and liver from germ-free or SPF mice fed with control diet (AIN93G, 20 protein) or low-protein diet(LP, 7% protein).
Experimental Design	stimulus or stress design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Tanoue
Person First Name	Takeshi
Person Affiliation	Keio University
Person Roles	submitter
Public Release Date	2026-06-03
PubMed ID	41781619
Protocol Name	P-GEAD-3432	P-GEAD-3433	P-GEAD-3434	P-GEAD-3435	P-GEAD-3436
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Approximately 50 mg of mouse iWAT around the inguinal lymph nodes or liver were snap frozen with liquid nitrogen	10x Chromium Nuclei Isolation kit (for iWAT) or Singulator 100 with the inbuilt program Single-Shot Standard Nuclei Isolation V2 (for liver)	ChromiumNextGEMSingleCell3'_GeneExpression_v3.1_Dual Index (for iWAT) or ChromiumNextGEMSingleCell3'_GeneExpression_v3.1_Singlel Index (for liver)	Illumina NovaSeq 6000	CellRanger, cellbender
SDRF File	E-GEAD-888.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA from single cells
Comment[BioProject]	PRJDB19694
Comment[Last Update Date]	2026-06-03