Source Name Characteristics[sample_name] Characteristics[organism] Characteristics[taxonomy_id] Characteristics[isolate] Characteristics[biomaterial_provider] Characteristics[collection_date] Characteristics[geo_loc_name] Characteristics[age] Characteristics[cell_line] Characteristics[cell_type] Characteristics[culture_collection] Characteristics[disease] Characteristics[disease_stage] Characteristics[phenotype] Characteristics[sample_type] Characteristics[sex] Characteristics[tissue] Characteristics[treatment] Characteristics[race] Comment[BioSample] Comment[sample_title] Comment[description] Protocol REF Protocol REF Protocol REF Protocol REF Extract Name Material Type Comment[LIBRARY_LAYOUT] Comment[LIBRARY_SELECTION] Comment[LIBRARY_SOURCE] Comment[LIBRARY_STRATEGY] Comment[INSTRUMENT_MODEL] Protocol REF Assay Name Comment[SRA_EXPERIMENT] Comment[SRA_RUN] Technology Type Array Data File Protocol REF Protocol REF Derived Array Data File Factor Value[genetic_modification] Factor Value[antibody] K562_NT1 K562_NT1 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) Caucasian SAMD00858625 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #1 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT1_IgG-ChIP DRX608872 DRR628544 sequencing assay DRR628544 P-GEAD-3517 P-GEAD-3519 K562_NT1_IgG-ChIP_FE.bdg control IgG K562_NT2 K562_NT2 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) Caucasian SAMD00858626 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #2 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT2_IgG-ChIP DRX608873 DRR628545 sequencing assay DRR628545 P-GEAD-3517 P-GEAD-3519 K562_NT2_IgG-ChIP_FE.bdg control IgG K562_ZFP91-KO1 K562_ZFP91-KO1 Homo sapiens 9606 clone 1A11 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858627 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #1) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO1_IgG-ChIP DRX608874 DRR628546 sequencing assay DRR628546 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO1_IgG-ChIP_FE.bdg ZFP91 knockout IgG K562_ZFP91-KO2 K562_ZFP91-KO2 Homo sapiens 9606 clone 4G1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858628 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #2) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO2_IgG-ChIP DRX608875 DRR628547 sequencing assay DRR628547 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO2_IgG-ChIP_FE.bdg ZFP91 knockout IgG K562_ZFP91-KO3 K562_ZFP91-KO3 Homo sapiens 9606 clone 5A1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858629 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #3) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO3 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO3_IgG-ChIP DRX608876 DRR628548 sequencing assay DRR628548 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO3_IgG-ChIP_FE.bdg ZFP91 knockout IgG K562_NT1 K562_NT1 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) Caucasian SAMD00858625 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #1 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT1_ZFP91-ChIP DRX608877 DRR628549 sequencing assay DRR628549 P-GEAD-3517 P-GEAD-3519 K562_NT1_ZFP91-ChIP_FE.bdg control ZFP91 K562_NT2 K562_NT2 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) Caucasian SAMD00858626 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #2 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT2_ZFP91-ChIP DRX608878 DRR628550 sequencing assay DRR628550 P-GEAD-3517 P-GEAD-3519 K562_NT2_ZFP91-ChIP_FE.bdg control ZFP91 K562_ZFP91-KO1 K562_ZFP91-KO1 Homo sapiens 9606 clone 1A11 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858627 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #1) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO1_ZFP91-ChIP DRX608879 DRR628551 sequencing assay DRR628551 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO1_ZFP91-ChIP_FE.bdg ZFP91 knockout ZFP91 K562_ZFP91-KO2 K562_ZFP91-KO2 Homo sapiens 9606 clone 4G1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858628 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #2) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO2_ZFP91-ChIP DRX608880 DRR628552 sequencing assay DRR628552 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO2_ZFP91-ChIP_FE.bdg ZFP91 knockout ZFP91 K562_ZFP91-KO3 K562_ZFP91-KO3 Homo sapiens 9606 clone 5A1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858629 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #3) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO3 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO3_ZFP91-ChIP DRX608881 DRR628553 sequencing assay DRR628553 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO3_ZFP91-ChIP_FE.bdg ZFP91 knockout ZFP91 K562_NT1 K562_NT1 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) Caucasian SAMD00858625 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #1 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT1_H3K4me3-ChIP DRX608882 DRR628554 sequencing assay DRR628554 P-GEAD-3517 P-GEAD-3519 K562_NT1_H3K4me3-ChIP_FE.bdg control H3K4me K562_NT2 K562_NT2 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) Caucasian SAMD00858626 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #2 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT2_H3K4me3-ChIP DRX608883 DRR628555 sequencing assay DRR628555 P-GEAD-3517 P-GEAD-3519 K562_NT2_H3K4me3-ChIP_FE.bdg control H3K4me K562_ZFP91-KO1 K562_ZFP91-KO1 Homo sapiens 9606 clone 1A11 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858627 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #1) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO1_H3K4me3-ChIP DRX608884 DRR628556 sequencing assay DRR628556 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO1_H3K4me3-ChIP_FE.bdg ZFP91 knockout H3K4me K562_ZFP91-KO2 K562_ZFP91-KO2 Homo sapiens 9606 clone 4G1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858628 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #2) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO2_H3K4me3-ChIP DRX608885 DRR628557 sequencing assay DRR628557 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO2_H3K4me3-ChIP_FE.bdg ZFP91 knockout H3K4me K562_ZFP91-KO3 K562_ZFP91-KO3 Homo sapiens 9606 clone 5A1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858629 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #3) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO3 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO3_H3K4me3-ChIP DRX608886 DRR628558 sequencing assay DRR628558 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO3_H3K4me3-ChIP_FE.bdg ZFP91 knockout H3K4me K562_NT1 K562_NT1 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) Caucasian SAMD00858625 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #1 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #1 (CTGAAAAAGGAAGGAGTTGA) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT1_H3K27ac-ChIP DRX608887 DRR628559 sequencing assay DRR628559 P-GEAD-3517 P-GEAD-3519 K562_NT1_H3K27ac-ChIP_FE.bdg control H3K27ac K562_NT2 K562_NT2 Homo sapiens 9606 not applicable JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis GFP positive, fluorescence intensity decreases after EVI1 knockdown cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) Caucasian SAMD00858626 K562-EVI1-GFP cells transduced with Cas9 and non-targeting sgRNA #2 K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and non-targeting sgRNA #2 (AAGATGAAAGGAAAGGCGTT) P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_NT2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_NT2_H3K27ac-ChIP DRX608888 DRR628560 sequencing assay DRR628560 P-GEAD-3517 P-GEAD-3519 K562_NT2_H3K27ac-ChIP_FE.bdg control H3K27ac K562_ZFP91-KO1 K562_ZFP91-KO1 Homo sapiens 9606 clone 1A11 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858627 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #1) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #1 (GGGTGTTCTCTGTATCACGA), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO1 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO1_H3K27ac-ChIP DRX608889 DRR628561 sequencing assay DRR628561 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO1_H3K27ac-ChIP_FE.bdg ZFP91 knockout H3K27ac K562_ZFP91-KO2 K562_ZFP91-KO2 Homo sapiens 9606 clone 4G1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858628 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #2) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #2 (TCCTACTACTACGCCAGCCC), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO2 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO2_H3K27ac-ChIP DRX608890 DRR628562 sequencing assay DRR628562 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO2_H3K27ac-ChIP_FE.bdg ZFP91 knockout H3K27ac K562_ZFP91-KO3 K562_ZFP91-KO3 Homo sapiens 9606 clone 5A1 JCRB Cell Bank missing: lab stock missing: lab stock 53 K562 myeloid leukemia cell JCRB:0019 chronic myelogenous leukemia blast crisis ZFP91 knockout, decreased cell proliferation cell culture female pleural effusion genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), followed by selection for single-cell clones where ZFP91 knockout was confirmed Caucasian SAMD00858629 K562-EVI1-GFP cells with ZFP91 knockout (single-cell clone #3) K562 cells with a P2A-EGFP sequence inserted at the EVI1 stop codon, transduced with Cas9 and ZFP91-targeting sgRNA #3 (CTGAGACATGCCTTTCTAAG), and selected for single-cell clones where ZFP91 knockout was confirmed by Western blot analysis P-GEAD-3513 P-GEAD-3514 P-GEAD-3515 P-GEAD-3518 K562_ZFP91-KO3 genomic DNA PAIRED ChIP GENOMIC CH_IP_SEQ NextSeq 2000 P-GEAD-3516 K562_ZFP91-KO3_H3K27ac-ChIP DRX608891 DRR628563 sequencing assay DRR628563 P-GEAD-3517 P-GEAD-3519 K562_ZFP91-KO3_H3K27ac-ChIP_FE.bdg ZFP91 knockout H3K27ac