Comment[GEAAccession]	E-GEAD-909
MAGE-TAB Version	1.1
Investigation Title	Transcriptional profiling of Ciona body wall muscles
Experiment Description	To investigate the expression level of muscle related genes expressed in Ciona body wall muscle, we performed RNA sequencing of three adult Cicona robusta body wall muscle tissues. The RNA-seq read were mapped to reference genomet, counted read and normalized with TPM method.
Experimental Design	cell type comparison design
Experimental Factor Name	individual
Experimental Factor Type	individual
Person Last Name	Nishino	Nishino
Person First Name	Junko	Atsuo
Person Affiliation	Hirosaki Univ.	
Person Roles	submitter	submitter
Public Release Date	2026-03-05
Protocol Name	P-GEAD-3565	P-GEAD-3566	P-GEAD-3567	P-GEAD-3568	P-GEAD-3569
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Adult individuals were provided by NBRP Ciona intestinalis. Body-wall muscles were dissected by scissors.	RNA was extracted by Chomczynski's APGC extraction method, followed by purified by spin-column.	Quality check of RNA sample, mRNA enrichment usingoligo(dT) beads, and the double-stranded cDNA library were performed by Filgen, Inc. as contact service.	NovaSeq 6000, PE150	Quarity check for RNA-seq reads was performed by FastQC. We used for mapping RNA-seq reads to reference genome with STAR program, counting and normalization for rsem program.
SDRF File	E-GEAD-909.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB20144
Comment[Last Update Date]	2026-03-05