Comment[GEAAccession]	E-GEAD-991
MAGE-TAB Version	1.1
Investigation Title	Expression profile of xenopus animal cap explants with activin/retinoic acid treatment using xenopus 22K microarray (CBX126)
Experiment Description	Changes in the gene expression patterns in the animal cap explants of xenopus laevis with the activin and retinoic acid treatment were analyzed using the xenopus 22K microarray. The up- and down-regulated genes more than two fold changes in expression level in response to activin and retinoic on 24 hours after treatment.
Experimental Design	dye swap design	development or differentiation design
Experimental Factor Name	sample_name
Experimental Factor Type	sample_name
Person Last Name	Asashima	Chan
Person First Name	Makoto	Techuan
Person Affiliation	The University of Tokyo	
Person Roles	submitter	
Public Release Date	2025-01-31
Protocol Name	P-GEAD-4218	P-GEAD-4219	P-GEAD-4220	P-GEAD-4221	P-GEAD-4222	P-GEAD-4223	P-GEAD-4224	P-GEAD-4225
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol	growth protocol	treatment protocol
Protocol Description	22K xenopus laevis microarray was customized by Agilent Technologies (Palo Alto, CA)., Animal cap explants in isogen and then kept frozen stock at -80 degree_C	Each total RNA was isolated from the activin/retinoic acid treated animal cap explants or activin treated animal cap explants using Isogen (Nippon Gene, Tokyo) following the manufacture's instruction.	The Cy3 CTP- or Cy5 CTP-labeled cRNA, called \"array target\" were prepared using the Low RNA Input liner amplification and labeling kit (Agilent Technologies) following the manufacture's instruction.	According to the manufacturer's instructions (Agilent Technologies)	scanned on an Agilent G256AA microarray scanner	Analyzed by Feature Extraction Software (Agilent technologies)	Cultured at Steinberg's solutioin for 24 hours.	Treated with 10 ng/ml of activin and 10^4M of retinoic acid for 3 hours., Treated with 10 ng/ml of activin for 3 hours.
SDRF File	E-GEAD-991.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-GEAD-196
Comment[AEExperimentType]	transcription profiling by array
Comment[SecondaryAccession]	CBX126
Comment[BioProject]	PRJDB20092
Comment[CIBEX Accept Date]	2010-01-01
Comment[Last Update Date]	2025-01-31