LOCUS       ACAAA0000000                       mRNA    linear   VRT 13-DEC-2007
DEFINITION  Oryzias latipes 5' cDNA fragments, 0-7 day embryo and adult body
            tissues short transcripts tag.
ACCESSION   ACAAA0000000
VERSION     ACAAA0000000.1
KEYWORDS    MGA; 5'-end tag; 5'-SAGE.
SOURCE      Oryzias latipes
  ORGANISM  Oryzias latipes
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
            Acanthomorpha; Acanthopterygii; Percomorpha; Atherinomorpha;
            Beloniformes; Adrianichthyidae; Oryziinae; Oryzias.
REFERENCE   1
  AUTHORS   Hashimoto,S., Ahsan,B., Takeda,H., Morishita,S. and Kohara,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2006) to the DDBJ/EMBL/GenBank databases.
            Contact:Budrul Ahsan
            University of Tokyo, Department of Computational Biology; 5-1-5
            Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
REFERENCE   2  
  AUTHORS   Kasahara,M., Naruse,K., Sasaki,S., Nakatani,Y., Qu,W., Ahsan,B.,
            Yamada,T., Nagayasu,Y., Doi,K., Kasai,Y., Jinbo,T., Kobayashi,D.,
            Shimada,A., Toyoda,A., Kuroki,Y., Fujiyama,A., Sasaki,T.,
            Shimizu,A., Asakawa,S., Shimizu,N., Hashimoto,S., Yang,J., Lee,Y.,
            Matsushima,K., Sugano,S., Sakaizumi,M., Narita,T., Ohishi,K.,
            Haga,S., Ohta,F., Nomoto,H., Nogata,K., Morishita,T., Endo,T.,
            Shin-i,T., Takeda,H., Morishita,S. and Kohara,Y.
  TITLE     The medaka draft genome and insights into vertebrate genome
            evolution
  JOURNAL   Nature 447, 714-719 (2007)
REFERENCE   3  
  AUTHORS   Hashimoto,S., Suzuki,Y., Kasai,Y., Morohoshi,K., Yamada,T.,
            Sese,J., Morishita,S., Sugano,S. and Matsushima,K.
  TITLE     5'-end SAGE for the analysis of transcriptional start sites
  JOURNAL   Nat. Biotechnol. 22, 1146-1149 (2004)
COMMENT     The method first splits the mRNA into two aliquots and its cap
            structure is enzymatically replaced with two types of synthetic
            oligonucleotides containing MmeI, a type-IIS restriction
            endonuclease site, and the XhoI restriction enzyme site.
            Oligo-capping mRNA is then converted into first strand cDNA with a
            random adapter-primer. The second strand is synthesized using
            biotin-bound 5'-primer and random adaptor-primer by PCR. The
            double-stranded cDNA is then cleaved with MmeI, which cleaves 20
            bp away from its recognition site. After the 5'-cDNAs are isolated
            by binding to streptavidin beads, the two pools of tags are
            ligated to each other. The subsequent procedure for
            concatemerization is carried out according to the original SAGE
            protocol
            Please visit our web site for further details of the method
            URL:http://5sage.gi.k.u-tokyo.ac.jp
            URL:http://medaka.utgenome.org
FEATURES             Location/Qualifiers
     source          
                     /db_xref="taxon:8090"
                     /dev_stage="mixture of 0-7 day old embryo and adult"
                     /mol_type="mRNA"
                     /organism="Oryzias latipes"
                     /strain="Hd-rR"
                     /tissue_type="mixture of embryo and whole body"
MGA         ACAAA0000001-ACAAA0356693
            total number of count : 1127683
            Header Format
            >[ACC#]|[submitter's identifier]|[number of sequence
            count]|[map]|[free text]|[db_xref1(,db_xref2,...)]|
//