LOCUS       AEAAA0000000                       mRNA    linear   HUM 05-DEC-2011
DEFINITION  Homo sapiens 5' cDNA fragments, 5'SAGE tags from embryonic kidney
            cells.
ACCESSION   AEAAA0000000
VERSION     AEAAA0000000.1
KEYWORDS    MGA; 5'-end tag; 5'-SAGE.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1
  AUTHORS   Hashimoto,S. and Nakatani,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Yoichiro Nakatani
            University of Tokyo, Department of Computational Biology; 5-1-5
            Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
REFERENCE   2  
  AUTHORS   Hashimoto,S., Ito,D., Kasai,Y., Nakatani,Y., Houdo,Y., Lee,Y.,
            Yamada,T., Ahsan,B., Yang,J., Kawakami,Y., Ametani,A., Sugano,S.,
            Morishita,S. and Matsushima,K.
  TITLE     Coordinated regulation of coding and noncoding RNAs through
            epigenetic modification.
  JOURNAL   Unpublished (2007)
REFERENCE   3  
  AUTHORS   Hashimoto,S., Suzuki,Y., Kasai,Y., Morohoshi,K., Yamada,T.,
            Sese,J., Morishita,S., Sugano,S. and Matsushima,K.
  TITLE     5'-end SAGE for the analysis of transcriptional start sites
  JOURNAL   Nat. Biotechnol. 22, 1146-1149 (2004)
COMMENT     The method first splits the mRNA into two aliquots and its cap
            structure is enzymatically replaced with two types of synthetic
            oligonucleotides containing MmeI, a type-IIS restriction
            endonuclease site, and the XhoI restriction enzyme site.
            Oligo-capping mRNA is then converted into first strand cDNA with a
            random adapter-primer. The second strand is synthesized using
            biotin-bound 5'-primer and random adaptor-primer by PCR. The
            double-stranded cDNA is then cleaved with MmeI, which cleaves 20
            bp away from its recognition site. After the 5'-cDNAs are isolated
            by binding to streptavidin beads, the two pools of tags are
            ligated to each other. The subsequent procedure for
            concatemerization is carried out according to the original SAGE
            protocol
            Please visit our web site for further details of the method
            URL:http://5sage.gi.k.u-tokyo.ac.jp
FEATURES             Location/Qualifiers
     source          
                     /cell_line="HEK"
                     /cell_type="embryonic kidney cell"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /organism="Homo sapiens"
MGA         AEAAA0000001-AEAAA0026367
            total number of count : 35997
            Header Format
            >[ACC#]|[submitter's identifier]|[number of sequence
            count]|[map]|[free text]|[db_xref1(,db_xref2,...)]|
//