LOCUS AEAAE0000000 mRNA linear HUM 05-DEC-2011 DEFINITION Homo sapiens 5' cDNA fragments, 5'SAGE tags from colorectal cancer cells treated with trichostatin A (TSA). ACCESSION AEAAE0000000 VERSION AEAAE0000000.1 KEYWORDS MGA; 5'-end tag; 5'-SAGE. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 AUTHORS Hashimoto,S. and Nakatani,Y. TITLE Direct Submission JOURNAL Submitted (11-JUN-2007) to the DDBJ/EMBL/GenBank databases. Contact:Yoichiro Nakatani University of Tokyo, Department of Computational Biology; 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan REFERENCE 2 AUTHORS Hashimoto,S., Ito,D., Kasai,Y., Nakatani,Y., Houdo,Y., Lee,Y., Yamada,T., Ahsan,B., Yang,J., Kawakami,Y., Ametani,A., Sugano,S., Morishita,S. and Matsushima,K. TITLE Coordinated regulation of coding and noncoding RNAs through epigenetic modification. JOURNAL Unpublished (2007) REFERENCE 3 AUTHORS Hashimoto,S., Suzuki,Y., Kasai,Y., Morohoshi,K., Yamada,T., Sese,J., Morishita,S., Sugano,S. and Matsushima,K. TITLE 5'-end SAGE for the analysis of transcriptional start sites JOURNAL Nat. Biotechnol. 22, 1146-1149 (2004) COMMENT The method first splits the mRNA into two aliquots and its cap structure is enzymatically replaced with two types of synthetic oligonucleotides containing MmeI, a type-IIS restriction endonuclease site, and the XhoI restriction enzyme site. Oligo-capping mRNA is then converted into first strand cDNA with a random adapter-primer. The second strand is synthesized using biotin-bound 5'-primer and random adaptor-primer by PCR. The double-stranded cDNA is then cleaved with MmeI, which cleaves 20 bp away from its recognition site. After the 5'-cDNAs are isolated by binding to streptavidin beads, the two pools of tags are ligated to each other. The subsequent procedure for concatemerization is carried out according to the original SAGE protocol Please visit our web site for further details of the method URL:http://5sage.gi.k.u-tokyo.ac.jp FEATURES Location/Qualifiers source /cell_line="HT29-TSA" /cell_type="colorectal cancer cell" /db_xref="taxon:9606" /mol_type="mRNA" /note="colorectal cancer cells treated with trichostatin A (TSA)" /organism="Homo sapiens" MGA AEAAE0000001-AEAAE0023437 total number of count : 74154 Header Format >[ACC#]|[submitter's identifier]|[number of sequence count]|[map]|[free text]|[db_xref1(,db_xref2,...)]| //