LOCUS       AHAAB0000000                       RNA     linear   INV 29-OCT-2009
DEFINITION  Bombyx mori RNA, BmN4-derived non-coding small RNAs.
ACCESSION   AHAAB0000000
VERSION     AHAAB0000000.1
KEYWORDS    MGA; small RNA.
SOURCE      Bombyx mori
  ORGANISM  Bombyx mori
            Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
            Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia;
            Bombycoidea; Bombycidae; Bombycinae; Bombyx.
REFERENCE   1
  AUTHORS   Kawaoka,S., Hayashi,N., Shimada,T. and Katsuma,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2009) to the DDBJ/EMBL/GenBank databases.
            Contact:Shinpei Kawaoka
            Graduate School of Agricultural and Life Sciences, University of
            Tokyo, Department of Agricultural and Environmental Biology; Yayoi
            1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
REFERENCE   2  
  AUTHORS   Kawaoka,S., Hayashi,N., Suzuki,Y., Abe,H., Sugano,S., Tomari,Y.,
            Shimada,T. and Katsuma,S.
  TITLE     The Bombyx ovary-derived cell line endogenously expresses
            PIWI/PIWI-interacting RNA complexes
  JOURNAL   RNA 15, 1258-1264 (2009)
COMMENT     Total RNAs were prepared using Trizol reagent (Invitrogen)
            according to the manufacturer's protocol. For small RNA
            preparation, miRVana(TM) miRNA isolation kit (Ambion) was used
            according to the manufacture's protocol.
            Ten micrograms of total RNA was loaded onto a 15% denaturing
            polyacrylamide gel containing 10 M urea, followed by staining with
            SYBRGold (Invitrogen) and signals were visualized using LAS-1000
            (Fujifilm). Small RNA libraries were constructed using Small RNA
            cloning kit (TaKaRa). DNA sequencing was performed using Solexa
            (Illumina).
            We cloned small RNAs of 23-30 nucleotides. Solexa sequencing
            generated reads of up to 36 nucleotides in length. 3' adaptor
            sequences were identified and removed, allowing for up to 2
            mismatches. Reads without adaptor sequences were discarded. The
            alignment to the Bombyx mori genome (Build2) was performed using
            SOAPaligner/soap2, allowing for up to 2 mismatches.
FEATURES             Location/Qualifiers
     source          
                     /cell_line="BmN4"
                     /db_xref="taxon:7091"
                     /mol_type="transcribed RNA"
                     /organism="Bombyx mori"
MGA         AHAAB0000001-AHAAB0547473
            total number of count : 1253793
            Header Format
            >[ACC#]|[submitter's identifier]|[number of sequence
            count]|[map]|[free text]|[db_xref1(,db_xref2,...)]|
//