LOCUS       AHAAC0000000                       RNA     linear   INV 29-OCT-2009
DEFINITION  Bombyx mori RNA, BmN4-derived BmAgo3-bound piRNA.
ACCESSION   AHAAC0000000
VERSION     AHAAC0000000.1
KEYWORDS    MGA; small RNA.
SOURCE      Bombyx mori
  ORGANISM  Bombyx mori
            Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
            Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia;
            Bombycoidea; Bombycidae; Bombycinae; Bombyx.
REFERENCE   1
  AUTHORS   Kawaoka,S., Hayashi,N., Shimada,T. and Katsuma,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2009) to the DDBJ/EMBL/GenBank databases.
            Contact:Shinpei Kawaoka
            Graduate School of Agricultural and Life Sciences, University of
            Tokyo, Department of Agricultural and Environmental Biology; Yayoi
            1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
REFERENCE   2  
  AUTHORS   Kawaoka,S., Hayashi,N., Suzuki,Y., Abe,H., Sugano,S., Tomari,Y.,
            Shimada,T. and Katsuma,S.
  TITLE     The Bombyx ovary-derived cell line endogenously expresses
            PIWI/PIWI-interacting RNA complexes
  JOURNAL   RNA 15, 1258-1264 (2009)
COMMENT     We immunopurified Bombyx mori Piwi (BmAgo3)-containing complexes
            from cells stably expressing FLAG-tagged BmAgo3 using anti-FLAG
            antibody. RNAs that associate with these complexes were isolated
            using miRVana(TM) miRNA isolation kit (Ambion).
            Isolated RNAs were loaded onto a 15% denaturing polyacrylamide gel
            containing 10 M urea, followed by staining with SYBRGold
            (Invitrogen) and signals were visualized using LAS-1000
            (Fujifilm). Small RNA libraries were constructed using Small RNA
            cloning kit (TaKaRa). DNA sequencing was performed using Solexa
            (Illumina).
            We cloned small RNAs of 23-30 nucleotides. Solexa sequencing
            generated reads of up to 36 nucleotides in length. 3' adaptor
            sequences were identified and removed, allowing for up to 2
            mismatches. Reads without adaptor sequences were discarded. The
            alignment to the Bombyx mori genome (Build2) was performed using
            SOAPaligner/soap2, allowing for up to 2 mismatches.
FEATURES             Location/Qualifiers
     source          
                     /cell_line="BmN4"
                     /db_xref="taxon:7091"
                     /mol_type="transcribed RNA"
                     /organism="Bombyx mori"
MGA         AHAAC0000001-AHAAC1704525
            total number of count : 5137677
            Header Format
            >[ACC#]|[submitter's identifier]|[number of sequence
            count]|[map]|[free text]|[db_xref1(,db_xref2,...)]|
//