LOCUS AMAAB0000000 RNA linear INV 05-JAN-2010 DEFINITION Drosophila melanogaster RNA, Aub-binding small RNAs, genotype: yellow white (y w). ACCESSION AMAAB0000000 VERSION AMAAB0000000.1 KEYWORDS MGA; small RNA. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 AUTHORS Mituyama,T. TITLE Direct Submission JOURNAL Submitted (03-DEC-2009) to the DDBJ/EMBL/GenBank databases. Contact:Toutai Mituyama National Institute of Advanced Industrial Science and Technology (AIST), Computational Biology Research Center (CBRC); Aomi 2-4-7, Koto-ku, Tokyo 135-0064, Japan URL :http://www.cbrc.jp/ REFERENCE 2 AUTHORS Nishida,K.M., Okada,T.N., Kawamura,T., Mituyama,T., Kawamura,Y., Inagaki,S., Huang,H., Chen,D., Kodama,T., Siomi,H. and Siomi,M.C. TITLE Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlines JOURNAL EMBO J. 28, 3820-3831 (2009) COMMENT Aubergine (Aub) was immunoprecipitated from drosophila ovaries (wildtype) using anti-Aub antibody immobilized on Dynabeads Protein G (Invitrogen). The buffer used was Binding buffer. After incubation with ovary lysate, the bead fractions were extensively washed with Binding buffer and the protein pools bound to the beads were eluted with SDS-PAGE sample buffer. After immunoprecipitation, total RNAs were isolated from the immunopurified complexes by phenol:chloroform extraction and precipitated with ethanol. With the total RNAs, deep sequencing (Takara Bio inc. cDNA library preparation and sequencing service Illumina GAII) was performed. 40,000 sequences were sampled from wildtype raw reads. Among the sampled sequences, sequences from 20 to 30 bases were selected and used for subsequent analyses. FEATURES Location/Qualifiers source /db_xref="taxon:7227" /mol_type="transcribed RNA" /note="genotype: yellow white (y w)" /organism="Drosophila melanogaster" /tissue_type="ovary" MGA AMAAB0000001-AMAAB0024086 total number of count : 35575 Header Format >[ACC#]|[submitter's identifier]|[number of sequence count]|[map]|[free text]|[db_xref1(,db_xref2,...)]| //