LOCUS       ANAAA0000000                       RNA     linear   ROD 22-MAR-2016
DEFINITION  Mus musculus RNA, small RNAs expressed in whole brain on 4 day
            neonate, strain: C57BL/6CrSlc.
ACCESSION   ANAAA0000000
VERSION     ANAAA0000000.1
KEYWORDS    MGA; small RNA.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1
  AUTHORS   Tanabe,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2010) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroyuki Tanabe
            Kinki University, Faculty of Agriculture; Naka-machi, Nara, Nara
            631-8505, Japan
REFERENCE   2  
  AUTHORS   Tanabe,H., Kawai,M., Suemitsu,R., Hattori,R. and Takeuchi,A.
  TITLE     Small RNAs in mouse brain specifically expressed at the end-life
            stage
  JOURNAL   Mem. Fac. Agr. Kinki Univ. 44, 115-122 (2011)
REFERENCE   3  
  AUTHORS   Tanabe,H., Kohno,N. and Yamaguchi,M.
  TITLE     Global profiling of gene expression in mouse astrocyte in response
            to the potential longevity determinant miR-29
  JOURNAL   Mem. Fac. Agr. Kinki Univ. 45, 1-16 (2012)
REFERENCE   4  
  AUTHORS   Tanabe,H. and Matsuura,Y.
  TITLE     Generation and some characterization of BAC transgenic mouse that
            neurally expresses antisense RNA against the potential longevity
            determinant miR-29
  JOURNAL   Mem. Fac. Agr. Kinki Univ. 46, 1-14 (2013)
REFERENCE   5  
  AUTHORS   Takeda,T. and Tanabe,H.
  TITLE     Lifespan and reproduction in brain-specific miR-29-knockdown mouse
  JOURNAL   Biochem. Biophys. Res. Commun. 471, 454-458 (2016)
COMMENT     Extraction of total RNA from the brain tissues and the subsequent
            isolation of a small RNA fraction of 18-50 nucleotides were
            carried out by using an RNAiso (TAKARA BIO) and a Small RNA Gel
            Extraction Kit (TAKARA BIO), respectively. Small RNA libraries
            were constructed from the small RNA fraction using a Small RNA
            Cloning Kit (TAKARA BIO), and then subjected to high-throughput
            pyrosequencing on a Genome Sequencer FLX System (Roche). After
            removing sequence artifacts generated in the library construction
            step, all the significant reads were mapped to the mm9 assembly of
            the mouse genome and to sequences with known function. The genome
            assembly and some functional annotation are available from the
            genome browser at the UCSC (http://genome.ucsc.edu). To identify
            small RNAs corresponding to microRNA (miRNA), Piwi-interacting RNA
            (piRNA), rRNA, tRNA, small nuclear RNA (snRNA), small nucleolar
            RNA (snoRNA), small cytoplasmic RNA (scRNA), miscellaneous RNA
            (miscRNA) and mRNA based on sequence similarity, we downloaded the
            sequences of these known RNAs from the following databases: miRNA,
            miR Base (ftp:// ftp.ac.uk/mirbase/sequences/13.0/mature.fa.gz,
            ftp://ftp.ac.uk/mirbase/sequences/13.0/hairpin.fa.gz); piRNA, NCBI
            Entrez Nucleotide Database (http://www.ncbi.nlm.nih.gov/entrez/
            query.fcgi?db=Nucleotide); mRNA, NCBI Reference Sequences
            (ftp://ftp.ncbi.nih.gov); other RNAs, Ensembl Genome Browser
            (ftp://ftp.ensembl.org/pub/release55). Then, BLASTN search was
            performed using our RNA sequences as queries and the downloaded
            sequences as a database. Since the sequences in the databases
            could not cover all of our RNA species, we aligned the query
            sequences with the database sequences using a 90% match criterion
            including gaps. If a small RNA had more than one annotation, we
            used the following order of priority: miRNA, piRNA, rRNA, tRNA,
            snRNA, snoRNA, scRNA, miscRNA and mRNA. Any un-annotated reads
            were classified as "match genome" or "no-match genome" when they
            were mapped to the mouse genome with a perfect or imperfect match,
            respectively.
FEATURES             Location/Qualifiers
     source          
                     /db_xref="taxon:10090"
                     /dev_stage="4 day neonate"
                     /mol_type="transcribed RNA"
                     /organism="Mus musculus"
                     /strain="C57BL/6CrSlc"
                     /tissue_type="whole brain"
MGA         ANAAA0000001-ANAAA0033164
            total number of count : 97725
            Header Format
            >[ACC#]|[submitter's identifier]|[number of sequence
            count]|[map]|[free text]|[db_xref1(,db_xref2,...)]|
//