===== ===== ===== ===== ===== ===== ===== ===== ===== ===== ===== ===== THIS DATABASE MAY BE COPIED AND REDISTRIBUTED WITHOUT PERMISSION ON THE CONDITION THAT ALL THE STATEMENTS IN THIS RELEASE NOTE ARE REPRODUCED IN EACH COPY. ===== ===== ===== ===== ===== ===== ===== ===== ===== ===== ===== ===== DDBJ Amino Acid Sequence Database (DAD) Release 4.0 July 31, 1998 including 325,816 entries, 99,852,596 residues This is a release of DDBJ Amino Acid Sequence Database (DAD). This database was created by extracting all translated sequences from DDBJ entries. This release was made from DDBJ release 34 (July 1998). 1. DAD Files DAD entries are stored in 16 separate files according to the organisms from which amino acid sequences are derived. These 16 divisions are the same as those of DDBJ DNA Database, except that all EST data are put into one file of DAD. Please refer to the release note of DDBJ for details (filename: ddbjrel.txt). Also, there are two types of DAD files for each division. Files with suffices ".DAD" are in a standard DAD format, whereas those with suffices ".DAD.fasta" are in a FASTA-compatible format. 2. Format of DAD Entries The standard format of DAD is almost the same as that of DDBJ nucleotide sequence database. There are, however, some important differences. Some of them will be described below. Accession numbers of DAD entries are written on the lines labeled as "ACCESSION." An accession number of DAD is comprised of a DDBJ accession number and a consecutive integer that begins from 1. These two numbers are combined by a hyphen (-). For example, amino acid sequences extracted from DDBJ entry D12345 have accession numbers D12345-1, D12345-2, etc. This number is quite useful for identifying all DAD entries. An amino acid sequence begins from the next line of "BEGIN." Up to sixty amino acids are written on one line. Following the amino acid sequence, there is a double slash (//), which means the end of the entry. LOCUS line contains locus name, length of protein, molecular type (this is always "PRT"), division name, and date of release of DNA counterpart. DEFINITION line contains species name and protein name. Other parts of DAD entries, including FEATURES, are almost the same as those of DDBJ entries, from which DAD entries were made. 3. A Sample of DAD Entries Below is a typical sample of DAD entries. This might be useful for understanding its format and contents. ----- ----- ----- ----- sample begin ----- ----- ----- ----- LOCUS AB000714 220 aa PRT HUM 27-OCT-1997 DEFINITION Homo sapiens RVP1 protein. ACCESSION AB000714-1 PID d1023862 SOURCE Homo sapiens tissue_lib:lung cDNA to mRNA. ORGANISM Homo sapiens Eukaryotae; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 AUTHORS Katahira,J. TITLE Direct Submission JOURNAL Submitted (26-JAN-1997) to the DDBJ/EMBL/GenBank databases. Jun Katahira, Institute for Microbial Diseases, Osaka University, Department of Bacterial Toxinology; 3-1, Yamadaoka, Suita, Osaka 565, Japan (E-mail:katahira@biken.osaka-u.ac.jp, Tel:81-6-879-8285, Fax:81-6-879-8283) STANDARD full staff_review REFERENCE 2 AUTHORS Katahira,J., Sugiyama,H., Inoue,N., Horiguchi,Y., Matsuda,M. and Sugimoto,N. TITLE Clostridium perfringens enterotoxin utilizes two structurally related membrane proteins as functional receptors in vivo JOURNAL J. Biol. Chem. 272, 26652-26658 (1997) STANDARD full staff_review COMMENT FEATURES Qualifiers source /organism="Homo sapiens" /sequenced_mol="cDNA to mRNA" /tissue_lib="lung" protein /gene="hRVP1" /transl_table=1 BEGIN 1 MSMGLEITGT ALAVLGWLGT IVCCALPMWR VSAFIGSNII TSQNIWEGLW MNCVVQSTGQ 61 MQCKVYDSLL ALPQDLQAAR ALIVVAILLA AFGLLVALVG AQCTNCVQDD TAKAKITIVA 121 GVLFLLAALL TLVPVSWSAN TIIRDFYNPV VPEAQKREMG AGLYVGWAAA ALQLLGGALL 181 CCSCPPREKK YTATKVVYSA PRSTGPGASL GTGYDRKDYV // ----- ----- ----- ----- sample end ----- ----- ----- ----- 4. Statistics of DAD The following are statistics of this release of DAD. total number of entries 325,816 total length of sequences 99,852,596 aa average length 306.5 aa name of longest sequence X90568-1 PID:g1212992 length of longest sequence 26,926 aa (X90568-1) ===================================== file entries peptides ===================================== ddbjbct 91,028 27,083,990 ddbjest 946 86,486 ddbjgss 20 665 ddbjhum 30,228 9,531,015 ddbjinv 36,695 13,777,032 ddbjmam 9,261 2,456,847 ddbjpat 3,239 879,334 ddbjphg 3,487 678,510 ddbjpln 48,476 18,565,387 ddbjpri 2,410 481,801 ddbjrod 26,885 8,100,151 ddbjsts 11 869 ddbjsyn 2,316 541,903 ddbjuna 680 222,521 ddbjvrl 57,044 13,925,969 ddbjvrt 13,090 3,520,116 ===================================== total 325,816 99,852,596 ===================================== DNA Data Bank of Japan Center for Information Biology National Institute of Genetics Mishima 411-8540, Japan Phone: +81 559 81 6853 FAX: +81 559 81 6849 E-mail: ddbj@ddbj.nig.ac.jp (for general inquiry) WWW: http://www.ddbj.nig.ac.jp (for DDBJ WWW server)