LOCUS       KCVB01000001            1308 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30pri ultra-conserved element locus
            uce-936, sequence.
ACCESSION   KCVB01000001
VERSION     KCVB01000001.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1308
                     /note="ultra-conserved element locus uce-936"
BASE COUNT          437 a          199 c          227 g          445 t
ORIGIN      
        1 aaaaaaaact gtttacactg ttcagtgctt tggggcatcc gaggactgta ttcatttgtt
       61 caaactgtaa tactgcattc atttacagga acagcagagg aacagttaga caacagtgat
      121 tcatgtgtat atactgttta ttaatgtcaa tattatgtct tgtttggaac gatgtgtaaa
      181 aattgtttaa gaatattaag atattgttta tgccttagaa tgattgtagc attctatttt
      241 agtgaacgtg atgaaaacat tatcataaat attgtttgta cagtatatac atatcctctg
      301 caaacactgt ggtatcctta atcttggtag tgcctaccct tccaacaggg gcatgtaggg
      361 gatgttattg ttttagtttt cattcttatg acaacattat ttttttatat gatttcaatc
      421 caacaaggcc tccaaagttg gacagtgaca cagaaatcat tcctcaccat aaaagaagaa
      481 aaaaggaaca acaagcattc agttatgctt ccacgatgca tttcctcgta atctgccaca
      541 atacagagga cctaaggcca tttgtaacca ctgtgttgaa ccttgctgtg tgttgtggcc
      601 tgatggaggc agctagattt ttttgtaccc aagtcaagag tacttgaagt tactttattt
      661 aagatattgt ggaataaaag tatcattctt ttgtaggagc tttatttttc actagtgtgt
      721 ggcacggacc tattaaaagg atgtttttca acgtaaatgt ctcaaatttc ttttttattt
      781 ccgttccctc caacagctgc ttctgataaa aggtgttgtg tggtgaatat gtggatttaa
      841 ccttggttat tcttttaaat tagaaatcac gtgtgcattc atgactacag tttgattctt
      901 agttcagttt caaggtgtag actattgcat atttaacaaa gttagtatca tagatcattc
      961 aataccttac acacaaataa ctaagactaa gactgccaaa aacacaagca tgaaaccgtt
     1021 tcagtgtcct aaaaatgtgt gttacaatta tgcaaataaa aaaagaaaat tactgacaaa
     1081 aatgttaata atgggcaaaa aaagtctcag agcttaatcc aaactgtatt tatcatatga
     1141 aaaattaact attttcaatt agattatttt aaaataataa atttagaatt tagttttcat
     1201 gttgtgttgg aagtagtggc agcatatttt ttaaatatgg atgacaaagg tagtttcaaa
     1261 caacatgcaa gcagaagtac agcataaaga aaatatgcta acaattat
//
LOCUS       KCVB01000002            1878 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 64pri ultra-conserved element locus
            uce-175, sequence.
ACCESSION   KCVB01000002
VERSION     KCVB01000002.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="64pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1878
                     /note="ultra-conserved element locus uce-175"
BASE COUNT          638 a          362 c          324 g          554 t
ORIGIN      
        1 aaaattaaag tgttgaattt atgaggatat aaagtcttct tgtttcttcc tgtaatcagc
       61 aaaaaattgt tttttctgac ctgaaaaatg agcggggcaa caaattatca taaaaactgg
      121 tgttgttcaa tattctgtcc aactaatcaa taattcaacc atattggaaa ttctattaca
      181 aactgtttta tcacaacaat ctcttgtctt tttatatttc ttcagatcaa ttcgtgctgc
      241 caaagattaa actgacaaaa aagctcaaat tacacttttt tcattgtcaa agctgccaaa
      301 ggtcacatga tttaaatttc atcatctgcc aaagtccacg agggtctgca aaaaatttat
      361 ggatgcagag agtaagagca ctgaccacac gcattgtctg catgtgtgaa cacgtccagc
      421 tctaccaggg ttgaggcgat gtaaacattg tcacaccggg gctcatatta agccataaat
      481 cagaaacaaa tatattttgt gcaaaaaaca aaaacgttac tgtaagcaca aagcttgctt
      541 atgcaaatcg tgtccagtga ctttaaagat tggcacctaa tgacagaggt tttctatatt
      601 aaacacacaa ttccagagat cagtgggaca cagctggtac agaagcacat gttctatctt
      661 ttttttttca gtacaaatga tgtgaaaatg gcccattatg gcaaggaaca aagaggatag
      721 gccccatgtt aaagccaacc cctcattgat gccctagtga ctcggccagc cactctccca
      781 aggcctactc cttgattaaa tgcataatcc actactcccc atccccaatg atttagtgtg
      841 cctttaaatc agcttgcgca cacataaaaa cctcaaataa aaatcaccaa gaaaaaccat
      901 tgcactctaa ctgttctatt aaaatgatac ccagcccagc ctttcttctc tgcaataccg
      961 tttattccac atgaatgcgt atcttcattt ttttcatagg tatgcatata aaatatttaa
     1021 agtttaaaat gtcaccgttg aaacatcaaa tgcaggagtc tctcattaat cagcaaggat
     1081 ggaggttctc ttttaaggtt ataacccttt gaaagagcaa aaattacatt tttgaccttg
     1141 aagatgagac aaactcataa acgtaaaata atttgcagtt taaaggggaa agagatatcg
     1201 gttacctcag ccagcagtgc tctgggttgt cagggggaga ccaaacaagt ctcattttca
     1261 tatttacatc caagctgttg agattaattt gaaatcacat caaggaagaa aaagaaacaa
     1321 aactcagaga aaacatcaaa aggggcaact gtcaaacttt caaatccatg gggccctcgc
     1381 tatctcccaa cgtgtgaaac gccgactttt ttcttacagt tttattaaat ggtgcatgac
     1441 attttcatta ctcaaggaaa ttaaattgac taattttctt acaagccctg tacgtctgct
     1501 attttaggga ggtgcacagt agagagcaat tgtttcaaca cctacacaaa agaaatgcaa
     1561 acgtggatga gctctcggaa gaaaattgat ttcaaagcct tataggaaat ttgttcttta
     1621 atgggggaat tcagggtttt gatcttttca ctgaggctgg gttttaatct tactgctgat
     1681 tcctgtttat tcacttcagt agaacataaa cacattcata aacagcattt acaataaaaa
     1741 tcagagtctc agttgttttt aattgtggca atagaatgtt gagaatttga aatatactgt
     1801 gcattttacc aatgatggtt aacagaggca ctgggatata atgagatgga atctcataat
     1861 ccaaaaaaga aaaacaaa
//
LOCUS       KCVB01000003            1718 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 97pri ultra-conserved element locus
            uce-973, sequence.
ACCESSION   KCVB01000003
VERSION     KCVB01000003.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="97pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1718
                     /note="ultra-conserved element locus uce-973"
BASE COUNT          590 a          304 c          335 g          489 t
ORIGIN      
        1 gggagggggg gtcatcagat aagagaagaa tttctagact aattaaaccc tttgagacaa
       61 agaatcacac cacagtgatt tacaactctc tgtaccagtt aacccgactc atatttatct
      121 gtcatattac tcagatacca tatctacctc tatgggtaaa aagtcttttt gtcctcaaca
      181 ttatggaaac acaagagggg aagatattag agtaaataac aacagctaca tgagatttaa
      241 attctaattt actcatgagt tttcaaagag acagctgcta aaaaggtaca caaagatcct
      301 tgccactgat tatctactta aacagtgaaa tggtataata ggctccttat tttaccctca
      361 gatacaacac aacttccttt gtaatgctta cattatagca tgcaaatctc attgtttata
      421 acacataaaa ataaaactaa actatacaaa ccatagacat tcttgtggcc ttgagtaact
      481 gattagagtg aaacatttaa tctaatcagt gtaacgcaac aaaacaaaag gagaagctcc
      541 aaacttgtga atagcaaagt ctaccaggca taattccttt tgctggctgc tgcagagggg
      601 tatgctaaga tagaaagtat tattttccta aatggaaggt gtcagatggt cacggaaatg
      661 tgatcggctg ttcaagatca ctgcttccca tctgcaattt gtgacgtgtg aaagattcag
      721 cccaaatgtg ctcaccaagc gtaaatcctc agcactttct cttatcgtgg aaaagcaaga
      781 taaccagctg tttacagatc agagaacata tcatattaat atcatggaga gggtgatatc
      841 atcttgtttg taaaatattt tgttaactaa gtgtggtgtg gtatggcaac ctataaagtg
      901 aaagttgtaa tttatgtgtt tgagaaactg atttagttta aaggtttttg tctgataaaa
      961 aatatttaca gggggaaaac agaagatgct gcaggaagcc tctttaaaga gataataaac
     1021 tgcaggttac agtaaataca aattacaaag cagcattata caagcagcta catttaaata
     1081 aagactcccc tcgattccag ttaactatat gtctaaacac aagcaggatt cctttgattc
     1141 aacttgtctt ttgtatgatg cgagaagagt tcacacttac atgtgaaatg atattataat
     1201 gtaatatgac tgctgtggat aaggaaacaa gcttaagggg tttcgattag gagttcctga
     1261 ttcagagtcc cagtaaactg caactttaac tgtttgtagt aaaggttgta acagtttgtc
     1321 accacattaa agtagtttga catgaaagta acaataagtt cacttgaaga aatgattttt
     1381 aaaagtaagg gcaaagttaa agggtcatta ggtgcaacat ttctaataac ggcgttactt
     1441 tatcccaaag aattttgata tacaacagac atatatttga atactgcaac tgattagcac
     1501 atgcttgtca tctcagatca aagtaagtca gaaatgattc ataaaaagtc cacagtgtaa
     1561 taaactggag gcacttatat ttgccattca gggagtatgg gaggcctgct gtttgaaacc
     1621 cctagatccc cacgcatggc gtagccacca cagacacggc tcagagagtc agccggcagc
     1681 cgggaggtga gcagaacagg cggtgtcccc ttcagagt
//
LOCUS       KCVB01000004            1051 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 164pri ultra-conserved element locus
            uce-1102, sequence.
ACCESSION   KCVB01000004
VERSION     KCVB01000004.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="164pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1051
                     /note="ultra-conserved element locus uce-1102"
BASE COUNT          326 a          162 c          263 g          300 t
ORIGIN      
        1 aaaaaaagga gtccggcgtt gctcaaactg tcagctccaa tgaaaagcat gcctgaaaac
       61 gttgtcactg aacccctgga caaggaaaag aaggggagtg ggcaatgaaa gacagggtga
      121 gagaagtggg ggagagagaa ggggaggggg gttagcagcc tccagagtga tttatttgcc
      181 aaatcaggac acaatgagtc gattatgagt cctctaataa gagagagtgt ttggggaccc
      241 actaattggg catgattgag ttgcagtctg ggagccgggc gaaaagggaa cctggtcggc
      301 ggctgtcatg caatcatcag ctaatcagag ctttgaaatt aaaactgcgt gtttagggta
      361 gaggacggga taatggcagg gacctgtctg agctctgagg ctgcctgagg aggctgttac
      421 cgtgtgtgtg tgtgtgttta tgtgtttgtt tgtgcatgtg tttataagta cagtatgtat
      481 gtttgtgtgc agttgataga gcaaattact gtatgcactg tgtgtgagag tgtgtgtgtg
      541 tttgaggcct cttatctctg actgtggtct gcgcgggtct tgatgaggac agagaaggag
      601 ctgtaatcag cccaaccctt gtattaagac tcatgatgtg tgtatatgtg tgtgtttccg
      661 gctttgtatc tccagtttca tgactcatat gtatttattt tataggaacg tagagccaga
      721 actgagaaaa ataatctaat acaacaacaa attggattta gttcacgtta atcagattaa
      781 tatgcatttc tttaaattag aaccaacatt tagaataatt tatacatgaa cagttaattc
      841 tagttttgtt aaatatgaaa atgtacaaca gtttattaat cttgttggtc tagatgaaaa
      901 aaaaaattac aaatcaacat ttttttaaaa agagatgtaa aagtttttcc cccatcacag
      961 ccacatgctg ataaagtccc acaggtctaa tacacttaag taaaataaga aactgaaaaa
     1021 tattaaagaa agagacactt cagacctgca t
//
LOCUS       KCVB01000005            1621 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 205pri ultra-conserved element locus
            uce-274, sequence.
ACCESSION   KCVB01000005
VERSION     KCVB01000005.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="205pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1621
                     /note="ultra-conserved element locus uce-274"
BASE COUNT          438 a          364 c          283 g          536 t
ORIGIN      
        1 aatacaggtt tttgttcaaa aatgtcatta ttgtcattgt ctccataaag acaacatgca
       61 tgacagcaaa gcatataaaa tactcccttt ataaagggag aaattaacat gcatgtgaat
      121 taaacatgca ccaacagcac agaatcaact actagaaatc tactattttc ctgacttaat
      181 gcagtgcaaa tcatatattg aaaggagtga aaagttgtct gccatcagct gtgtagatac
      241 atttccctga atatttttta ggagtcagtt taatttcatt acagcctgac ctgtgtgtgt
      301 ggaactgcat taacatttgc tgcagtttag agcatttcca tccagcacat tttctatttc
      361 attttaacat tccacaccaa cagacaaagt caaaggggtt tctgtgtgtt tgtggtaaca
      421 aatgatggtg tgaaatgaat ggaaaacgag acagtctgat gatcatgcgc agactcatat
      481 ttttagaata tccagtcagg gcgcagccta ttgggtgtca aataaaagca cctgtctcca
      541 tgacaaagtg cagcccattc tcctcagccc cagcctctgt cgactagacc ctatctagca
      601 tgtgtgtgtt tttctcccac caaacctact ttatacgctg gactttggag gccgtctgtc
      661 ccttttattt gcagttaatt tttgccgcct tcaccggggc tcttcaccat gaatcttcat
      721 ttcgccgctg acacccttga atgtgaattc ctcatttcct gtccttttat ttatatctgt
      781 tgcctggtga tttgatcatg ctgggaatac aagattagca attgccttgt ccttggtttt
      841 gcctcaagga catctgctcc tgaaatgtta ttgtttgtgc ttcttaaccc ttcatgtcct
      901 ggcttctttt tcacctcaga ttttgcacag tggatgcaat gctctctctc tctctctctc
      961 actctctctc tcttaatgca tttgtccttc acagtattac ctgggaacag gcacgaaact
     1021 gacaaatggc aattccacca ttaaaaatct gcccatttat tgtgtcagtc cacagctaat
     1081 tttttgattt gtttgtgcat tcatgtgttt aatcaaacgt gtcaccacgc aagacacata
     1141 attaacttga ggtaatgatg tcattatgga atgaaatgta gtaccctttc tgggcaataa
     1201 cacgctgatt gctctaatta ttctctttga ctagcactgt cacttattcc agcacaacaa
     1261 atagataaac ttgttaactg tgaatttaca gagtgtaacc aattcagcta ctgcgtgtta
     1321 atacccccaa tttcttgtct ttgtttaata gaacaacata taatggggta atgtttccac
     1381 aaccgctgac cagtaatcag ttaaatgtgt ttaaaatgaa aactctccat gcaagggcct
     1441 tattttcaat atacagcttc aacttctatc tctccctgct ctccatgtgt tgatgtatat
     1501 gctgttatgc ctttttcttt tcactggccg tcacgtcatc tctataggac cgatgaggtg
     1561 tgttctataa tgtcacagtc agaccctctg gagccatgca ggagcaccag aatgtaaaca
     1621 c
//
LOCUS       KCVB01000006            1160 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 246pri ultra-conserved element locus
            uce-231, sequence.
ACCESSION   KCVB01000006
VERSION     KCVB01000006.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="246pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1160
                     /note="ultra-conserved element locus uce-231"
BASE COUNT          328 a          183 c          259 g          390 t
ORIGIN      
        1 tgttgttgtt gttgttgttt tttctccaga ggaagcatac acacaatgta gcatcacaat
       61 atttattacc ctgtcacgat gttagcttgc cgagctgcag ggtgcaccga gcagccgaga
      121 gacatgtgga gcgaaagatt aaagtgggag ggggcaggaa atacgacgac acgacgaagg
      181 attcaaatga aggaaaaggg actgagctct ctgcacctta gccctgctgc ctccacggac
      241 tcggacctgc ctcctttttc cacacaaaac catcctttta gcttagagat actgatggga
      301 ggggagggtg ggggggacgg gagagaaaaa tgtattgttc tctttttttt tttgtttttt
      361 gttaacttgt acactgtgca aggttgaatt atcctgtaca gactgtaaaa tgtaatatta
      421 ttttgtacaa cttaattgaa agaaaaacaa atctacttgt agatctttgt gccttgatta
      481 tggctgtacc tgtaaatgag ctcagatgta agtatgtttt cgactgcgct gtacagcttg
      541 atgtcaatct ctatcagcag cggaagactg cgggtagggg actttctctg tagagtttag
      601 ttttttctgg tttataaaaa aaaggaaatg ctgtttagta aaaaataaaa aatagggaaa
      661 aaatcctaaa aacctgtata cattatgcaa attaaccact tacctgcagt gaagaccaga
      721 tgttgcagcg ccatgccttt tttttttctt ttgttgtttt tatttttcct cctttaattt
      781 tattttattt tattttattt tatttgaatt tcacagcttt aaacaaacat tggaaatcca
      841 ttgaaagtgt ccaaattgca acctggtgtt gttttaatag gaaccaacgt tttttcgata
      901 tgaagtgtgt ctgaggtctg tactgatgtg cgtgcctgtg cgcatgtgta cataaaacaa
      961 acatagactc acagacgcat tgtgtgtatg tgtgagtgtg tgtgcgcgag tgagtgtgag
     1021 ggcgagtgtg cattgtgtgt aaaattttat aaatgtatgt acatgtaaat ttataggtct
     1081 atataaatat atatgtacaa ttatacatgt ttaattatgt gtgtgcctag gtgtacatac
     1141 ctatgtatgt atacggtaaa
//
LOCUS       KCVB01000007            1010 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 291pri ultra-conserved element locus
            uce-559, sequence.
ACCESSION   KCVB01000007
VERSION     KCVB01000007.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="291pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1010
                     /note="ultra-conserved element locus uce-559"
BASE COUNT          302 a          269 c          197 g          242 t
ORIGIN      
        1 aaaaaaaaaa aaaaagatct gtgcacacat gcccgtctaa tttggccttt taaaaccctt
       61 aaaatacatc aagtcatccc aggaagagag accatcttct ctgcaacaat tacttcaaga
      121 gctccctctt tcttccctac atttctcctt ttctcccctc tctttctttt ttgctgcctg
      181 acttttacaa cttcctcctg gttggggctg cagctctaac ccaaacaaaa ctccattttt
      241 tagttattgg ccctcgtggc atgtaatgga gaatcaagct aattatccct acagctgttt
      301 tcacttgctt tttatgtaag caatacctgg ctgatccaga gagcaacagt gaggcccttc
      361 ttagcgctcc aggcctccag gccggctgga attagagagc aaaatgccag gttttcctcc
      421 cccccgatac cgcccgtgtg tacccctgca cccatctccc agcacacaac ccaaaccaac
      481 caaagcccag cccgacgcaa ccctgtgcac acccaaagct tccatttcca ctccctcata
      541 cacctctcta aactccctct ttgatgttaa acaaagctgc cgttcaacag ttaagatgga
      601 tcagtatgac aagtgctcat tttcttccac tgatggttgt tggcaattaa tgaaggaggt
      661 gagccagggt gtgtctggga ttaaaatgta accatctcct gctgtcatga gacaagaaag
      721 caagtgaatg cataaaagaa gagggaggga aagaaagaca gagtggcaaa ggcaggagag
      781 tgggagagaa gagggcaaag agtgaaagaa aacaatccct caaaggctaa ttacataatt
      841 tccttcatct gagcaaacac atgctgggtg aaagcaagtg tgagcaaacc tcacagatgc
      901 caggcaaaga actgctgagc agacctacta acacagtcta caactgctct ctagtaatgg
      961 acagcctgtg ccacagaggc accaagccag atctcaaatg caacattatc
//
LOCUS       KCVB01000008            1796 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 331pri ultra-conserved element locus
            uce-1246, sequence.
ACCESSION   KCVB01000008
VERSION     KCVB01000008.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="331pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1796
                     /note="ultra-conserved element locus uce-1246"
BASE COUNT          520 a          386 c          363 g          527 t
ORIGIN      
        1 ctattcacca gctgttctgc cagctgccac tctccggctt aaggggagat tgcagttttg
       61 ggggctctga gtactggttt atcttggctc ctcatctgct cctcacgaag ttttgattgg
      121 attttttttt ttccttgccc tcctcctact tccaggttcc ttgagttgca tactttcctg
      181 caatctttcc ctttacttct ttgcttttgt ttctcccccc ctctaatttg tgtgtagata
      241 agagactgca atttgacttt gaatcttggc tttttccggc ccatgtcttt tgcaaggcct
      301 atacaagtct tcagcagtat tttttttttt caggatatgc tcttttaata tgctcagaat
      361 aattattaca aacatcaaca ccaaagcctt tctccaagga agtaaagcac agaagttagt
      421 gatgcacatc acttcttagc atgcagcaca ctagcctctt gtcagccctt gaacttctcc
      481 cactctttct cccaagaaaa gtatttgtaa ggaatcacag cactgaaacc tgctcacccc
      541 acagtcatct aacagcattt ctgtaggatt agcctggaca tggcctcagc ctgtacagtt
      601 atgattattg acatattggg tctgacatat ttcacattta tgtgcattgt ctggaagcat
      661 actcaagtgt tctcaatgtc atttctgctg aagtacggcc gggcagtctg cgagcaaggg
      721 ccgcaatgaa ttgatcattg ctcactgctc actttaataa ttcaagcctg attgccacag
      781 agtgccgcag taaaatggat tgggagaggt cacatgctgc tcagctcagg aggtgaagcc
      841 cctcggggat ggttaattca tatatgcttg atagacacgt gtcctattta gagaggtcag
      901 ggtgctgtgt gtgtatatgt cttctcagaa tggccaggtc accactgtgg gattaacacg
      961 ttggtcagtg cagataaagg cgagggataa ggaagaagtt tctggtcaca taggccagcg
     1021 agtttagccg cctgaggaaa aagctaaaat aatcaaaaag ttacttgcaa attgcatctg
     1081 attttcctac ataaaactac acagtttgta acagttttta cctgtggttg tatgtgtcga
     1141 ggtctttcag tccactcggc atttaactct gcaaccttca gtagctaatg gaatacaagg
     1201 aatagaaata aatttgctgg aagcaatcac aaaatgttcg gcaaatatca aagaaataga
     1261 gcagcagaaa aatagaccat tgaattgaac tgaattgatt ccttgaatac aaactatggt
     1321 aacaactttg tataagactg ttgatccaat atcagcttca cgctcagcct ccaaaaagca
     1381 gcctctcaaa cttctattca caaaaatgac ctttgacttc taaaaaacta caatagaata
     1441 taacagaatg gaattccttt attgtcagcg cacagagtac aaccaaattg actagcaaac
     1501 tgacccctga ccccttctac actctaacaa ctaatgttgg tacttggtga ggtatgtcat
     1561 catgttggat aaagacggta cagtaacgag gagggaaatt aggatgaaag taaatgaatc
     1621 agcaagggaa gacatgtaag aacctcagat gaagcacagc tcattcaggc tatctgccat
     1681 gtatttatag taactgttta catgaccaaa caggacagtc taaaggtgac taacagagat
     1741 gctcaccaag tggaataatg attaggtgtc agttaaagaa agcccaccat gatgtg
//
LOCUS       KCVB01000009            1285 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 359pri ultra-conserved element locus
            uce-369, sequence.
ACCESSION   KCVB01000009
VERSION     KCVB01000009.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="359pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1285
                     /note="ultra-conserved element locus uce-369"
BASE COUNT          348 a          325 c          228 g          384 t
ORIGIN      
        1 atctatctat ctatctatct atctatctat ctatctatct atctatctat ctatctatct
       61 gtgttataat tgcgagtaaa caaaagaaac tctctacagt cattataaac ccacccacag
      121 ctgccactgg gacctccatt aagcccagtg ttcctgagat gctctggtct tatctgattc
      181 atcaacaaga cgttggctgg catgatttat ctatgccact tacttgtttt cattttgccc
      241 gggggctgct gccagcatcg ggggcttctt tttacaatgt gacagctcca tctcaagaga
      301 aaagactccc catgcaaaaa cagtgcatca aaacaaataa ggcccgagcc acttaagaga
      361 agtggctgtg ttcgggaccc attttcactt cacgtcaacc aaatgatgca accgcacgca
      421 caaatctgca ttacttctgt gcttatgcct tttgtctgga tagtccatgt gtgagtctaa
      481 aaataggggt tgaatacaaa tatattccat tatgcatttg ttgtcgcttt tacagacttg
      541 accccaaaat tatgcacgat gatattttta tcgattatct gtatttttgt tctctgcagg
      601 cttccattta cccgtcttct ttgtgaaaat tctccacctc ctagtttacg tctcccttcc
      661 ccttctgtgc tccccaaatc tacaggcaac gggacgctct tgtacattaa ataagaactg
      721 tcaccctggg caacttgcag caatgaagca tttattctgc ctcacatttc ctggcactta
      781 gaattatttc cttcatgcct gataaccaaa ttacaatatg gcaggtctcc ccggagccac
      841 aggattgtta gtgccagctc acaacatact ttcaactgat gtggcatttc atgtacagct
      901 gccaatcaaa gccagcagaa gggagagatg taaatgaggg cgggcccgac ggtgttcaat
      961 taccgctctg agctctgcat aatccccctt cccactgact gactgactga cagctccaca
     1021 ttcctcctca tccccagctt tctcccctaa tttcactacc taggtttcgc ctcatactgg
     1081 gacatggaat cagacattgg agggaatttc tgtcatttcc atttccgttt agaatagcac
     1141 acaaaaaaaa aacgtattat ctgacatctg aaggactgta aaggaaagtc atgtgtattg
     1201 agagagtaaa ttattgctta caacaaaatt ccctgcaatt tgtgacccag agctcaaagc
     1261 taaacaaagt ctcctatagg ctgcg
//
LOCUS       KCVB01000010            1207 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 394pri ultra-conserved element locus
            uce-401, sequence.
ACCESSION   KCVB01000010
VERSION     KCVB01000010.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="394pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1207
                     /note="ultra-conserved element locus uce-401"
BASE COUNT          352 a          196 c          213 g          446 t
ORIGIN      
        1 ggaaacaact tgaaaagtgt gtcttcttga tttaacgaca gtaggagcag gtcatcgaaa
       61 aagtttcttt gtgcagcgtc aaaacttgct tgttggtcta ctatttttgc ccttattgta
      121 attatgctct ttgttcaaac tatgcactct ctatgcttat gaccactagt atctaccaca
      181 gaatttttac agcccttcat acataggttt gtcttgtttc ctttttttaa aatcttctaa
      241 tatgtgactt gaaccatttc tgttaagata actcgatatg cttgtagatc ttcggcttta
      301 tttttcattg tgatgcagta cgcccgaatt taacactttt tgctctcaat aaaaaaaaaa
      361 aaggacaaac aaatttttgt aaatttccag aaccagcagt gatgctgtca ttgttttgtt
      421 tggttttttt gttttatttt gtagatttcc atatgtctgt ctgtctgttt gttttcacag
      481 tcttgtcaaa cctgtgtaag gataagagga tagaagttat attggttttt atgtcaataa
      541 attcaagttt actgtggcca tccactcttg tatgtctttt gaagcattaa aacctatcgt
      601 gtatgcatga aattgtaaag ttctttctct tgttggtgta attttgtagc gtgacatgat
      661 ctccggtcca cgtctttatt catcaggata catcagtttt tccaatggtc atgtccacga
      721 tattgtgaag tggagaaaaa attctgaact tgtaactcta ccaaagccaa tgttcgtttg
      781 tagtatattt attgtattat ttaaaataaa aaaataaatg ttaaatgata caatagtgta
      841 aagtgtaaga ggccccctct ttacatgcac acaggagaac aaaagttact gtcaaaacct
      901 tattaattag tcacatttta caacatatga gctggaaatg acttctttta atgaagtatt
      961 tggccacatc cacttaaata gatcgtcctg catttatgtt aacaacctct gggctgtgtg
     1021 tgtgtgtgag aatgtgtgac aaaggtttaa gtaagactat taaaatgcta aataaatgat
     1081 tggatgtctg tttaagctgc agaacaggta aggctgcttt taattgcact acggtgtaag
     1141 ttttaatagt gtcaaataat tctttctcca cagatatttc aaacaaagaa gttctcaggt
     1201 tgcgtct
//
LOCUS       KCVB01000011            1252 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 434pri ultra-conserved element locus
            uce-893, sequence.
ACCESSION   KCVB01000011
VERSION     KCVB01000011.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="434pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1252
                     /note="ultra-conserved element locus uce-893"
BASE COUNT          360 a          220 c          251 g          421 t
ORIGIN      
        1 agggtccaga ggcgtgtccg aagaaaagct cccagacgta tggcaaggtt catacacgcc
       61 acattaacca gcttttcatc cggggtgaga atgtcgtcct ggttaaccca cagccattat
      121 gatttttgtt tttatttacc ctggatacac gaaaggtgtg ctacaacaat caatgaaatt
      181 taagttgtgt tttatgctga acatctgtaa tgttgaaaca ctgtagatga actgtgagag
      241 atgattttct tagattcaaa aagaatcaaa caaggactat tgttgtatca tattgatatg
      301 gaggtttttc tctgcacact ggagaagtta tctaaagtaa aattattata gcaatatctt
      361 gtcttgtttt cttttgtctg aaattcgttt tagactgtgc tataataact gtttgtgtaa
      421 tggttattat ccagcaggtg gcagcataaa cacattagtg ccatctgcga ggccattgtc
      481 atctcaggga gcttgtccct ttatgttgct tttcccgtta gtttgaacag gaatgacagc
      541 gcagtctcag cggacttcat aaatgcatta tgaataattg agtttgtgaa gctagagatt
      601 gcagatggca ctcaaaagct gtcatttgtc ttgacgtctc agattgcttt tactctttaa
      661 tgggcacatt acccataaaa gcacaattta ctgcatctcg tggaagttgt aaagatgttg
      721 cttttttcag ttttaaatgc cagaggttac ttttagatga tgagtgacac agagtgattg
      781 tgtgttgcta taatgttgct gcatctgagt cagttttatt tagtttattt tctttaattt
      841 gactgagttt aattgaccct ctttccatat atttttgtca taaataggac aaattctagc
      901 aggagctaag ctgttgttca cctatatacc tttcatataa tgtattagta ttattagtat
      961 agcccattta acagtcttcc tctccagaga gtaaaaatgg ggtcaattgt ttaatgcaag
     1021 ctgtgtttta gtggtgtttg tcacaccagt gtgtttcaac aaaccttcac acagcatgag
     1081 aagggcaaag cactggcact gaagagaggc cacaattaga ctacacggtc tctctttagt
     1141 ttaatattcc agagtaatca ggtcatccgt ctcactagaa cagtgtcaat ccgaccagat
     1201 gaatgaatat taaagcatta aaggctcaaa ctatcgacag ttctttagaa aa
//
LOCUS       KCVB01000012            1669 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 475pri ultra-conserved element locus
            uce-444, sequence.
ACCESSION   KCVB01000012
VERSION     KCVB01000012.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="475pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1669
                     /note="ultra-conserved element locus uce-444"
BASE COUNT          527 a          316 c          321 g          505 t
ORIGIN      
        1 tgattacact tgttattaaa tgtgagtgac attgatttac aaaatggcct gatataaggt
       61 taatggctga tgtcaccttc ttgcgcctct gtcttttctc aattatcaca aagctttgtt
      121 cagtgtgtca ttaatgtttc tatgtcctta agtagcatgg ccttctactg tatacatcct
      181 gccttcacct ctatacatta cactgcctct gtgcttgaac taccctctta tgccaggttt
      241 ctataattat gtaaggcaga aagtaattta aataggatga acagccataa cattggtgtg
      301 ccctggccca tttaagcaca tttacgtgag ccattattga cagacggcta agtgtcattt
      361 gtcaaggaag gaagaatcga cagggcaaag aaacagagtg aagtaacggt caatcagacc
      421 tactttacag tcataagaaa aaaaaagtgg acatctccag tgtgtttcca ttttcttctg
      481 ggaggaagtg taagatcata atacctattt tcataataat acaaacagcc tgtaggctac
      541 aaaataagaa gaaggccata ctctgtatat ctcggcattg cctgtcagag acagaggggt
      601 ttcaatctct ttttcatttc atcctgatag ctctgccttt tattacagag ggacaggaat
      661 gtttcctgag gtaaaaagga gtgcacattc ccaagataaa gttggaagtt tacagctatt
      721 tccagccgta atgtattatt ccgttaaagc aatgatcagg tagtgttgga tgaatgggag
      781 atgttcagca ccattactgc ctggcacatc agtgaggcat tattacatta tcgctgctct
      841 ttagtaggag attgggtgtg tgaccgggtt ctgaatacac tcatcccgct ctccacggcg
      901 taaattgcag attggctcat tgacttcaat gataaaatgt aaaaattgcc agtaaagaga
      961 acatagactc aggttactcc ttagctgttt gtcaacggca gatgtgactc agttggtgca
     1021 gagcatgtta aagagttcca tctcccagaa gtcaatgtca tttatgcctg aaactgctaa
     1081 agcaatagct catcttgtct ctggtgtgtc atactacact gcctttgtct gagattaaaa
     1141 ttttctccag tggtgggcta aaatcctaaa acgtcactta cagcatagag caaagatata
     1201 ctaacagatg taaccagatt atgttaatag gaaaaaaaaa aggattcgaa tttacatgat
     1261 ttactattta aaattaaaca cactaaagat ctgtcaagac caaaagaaag ttgttatctc
     1321 ctcacacaaa taattaacaa atacatgtca ccgacatctt tatgataatt tgtgtttgat
     1381 aattgacaga taattaatta agtaatgcat caaacatcca tcccacagac catgtgtttg
     1441 atgtgcgcgt tagtgccaca tgaaagaaaa acctagaagc attattattc tgcttattag
     1501 gcctgggcag gttattctgg aaatggatac tgctaatgtt atttcgcatg acactccgga
     1561 aaagcattaa tccatgataa ttaccttgga aggaagcaaa taaagacaaa ctaatggtgg
     1621 ggttgatcat caatttcaaa cattcgactc aaatgcccta catttttat
//
LOCUS       KCVB01000013            1552 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 523pri ultra-conserved element locus
            uce-91, sequence.
ACCESSION   KCVB01000013
VERSION     KCVB01000013.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="523pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1552
                     /note="ultra-conserved element locus uce-91"
BASE COUNT          484 a          395 c          308 g          365 t
ORIGIN      
        1 ccgactaaca ctcacagcca caatgcatca tcttcaatgc cttgacttct cttgagatga
       61 caaaaaactt tgactcaccg gctttctgca cctgcagctc tcttttggcc tgctccagaa
      121 tcgacttgat agcctcatct gaacccgcct ctggggtgcg gacgcgagca gtaatgttac
      181 ctggaaggag aggaagaggg aatgggagga gatgaaaaat tgtgtccgat taaaaagcag
      241 agataaccaa caataactga gcctataagg acatcaagta attacaccat agcacagcac
      301 aaagaacaac attcccacca ctgagctaca aggcagctac actggaggga ttgtcataat
      361 ttctctggtc cctctggagc tcacacatgc acacgtgcta cagggttcgt acagtaaatg
      421 ctccccacag aataacaact accaataaaa tccctaaata cacatgctgg cttttccttt
      481 gacgcaagtc actggaactg tgcggttacc ttcaaggcac atgtatatct gtgcgcctgc
      541 ccgcaaatta ataaacgcaa actctcacac agacacaaaa acacacacac acacacacac
      601 acgcccatca tagatacagt tcaccccaac acaaacatgc cacacagaca gaacgtgcta
      661 caatctctgc gtgtgaaggg gacagacctg tgtgtgaagc tgtattggag agggctctcc
      721 gcttcggaac atcctgaaac actcggctaa gctggggctg gcctgagccc tctgttaaaa
      781 acaatatacc acacactatg ttacagcact tgtgtgtttc ttttctttta cagctagact
      841 atatggtgcc ctatgatgct ctgcaaatat atggaatgtg ttgacacttc catccaatgc
      901 acattatagt actataaatg cattgatctt caccatctgt ggccccggta ggaatcaaac
      961 ctccatctct aacaggaata ataaactagt acaaagagta gtgagagata aaatatttaa
     1021 tagtacgtat aaaacagaac atacaaactg cagcctcatt ttttacatgg actaactatg
     1081 tctaggacta cggttgcaga ctgttacatt ttttgtacca ctgagaccac cttcaatcag
     1141 aaatgtcctg atgcgttaac aaaagcaggc ccaggctaat cgaatctacc cgtagttaac
     1201 agtacccttg ccttcctcct agttcccacc gatcaatact attgatgtgt gaaaggtttc
     1261 tgtattgact gcgatagcca ggagaggctc ttgggatcag tgcgtgacta ggaactcgct
     1321 caaaacacac aggtgtggca caggatggga gttaggaaag agaaagaaaa ataaaaagct
     1381 agtacatatt gacaagctaa tcaatggctc tgtgcagggc tgtacacaca cactcacaca
     1441 tgcaagacac atacacacac actctcacgc acgcactgac aaattaaaga tacacagact
     1501 cacctctctg ccgtccctgg atgctgcgca gcgcgaggat gttctgctca tc
//
LOCUS       KCVB01000014            1322 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 569pri ultra-conserved element locus
            uce-6, sequence.
ACCESSION   KCVB01000014
VERSION     KCVB01000014.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="569pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1322
                     /note="ultra-conserved element locus uce-6"
BASE COUNT          431 a          241 c          288 g          362 t
ORIGIN      
        1 ctggctcccc agccctctta attagcggct tccgtcagct ggcacccagg ccttcaagca
       61 ccagacactg gcctctgccg ctgaaggaat ccagttcact ccagatcttc agcttttcta
      121 acagatggat acatgggctg agtaagaggc atcaagtttt tttgcaaatc tgtgaatcat
      181 ctgtgaattt cctggtttcc acatcagatt tgtgctggaa ggaggagaaa atgctgtcga
      241 atgctttcat cattgtgttt cgtggtttta ataaaaatgg gcaacggcat agaacggacc
      301 aggagttttg gtctcagctg cagacacact gtaacaagac actgatgatt tttatatggt
      361 gaagtcaaat atagataaaa gctttcacca ggacgggaat gttgcctttt tcctggtgca
      421 gtatcatagc agtactgtaa caatagtctc ttctaatgac attcccctag taacttcttg
      481 cctctatgaa tctcaggggg gaagtgctac aaaaagtgat aattacatga aatatacata
      541 gagaataaag ggagcatgtt tacataacca ggcccataaa tatctgacgg agaagacatt
      601 gttctccgac tccttttaag ctgatctccc agggatgcca ttttaatgac atgatggttt
      661 aaagagatgc tgctccatct cgttccatat ttaattaact tttgcttttc tgacgcagag
      721 gaaaaacaga ataaagaacg tgtgatagag acaaacacac acgatagcga ggagacaata
      781 acaaatagct gggagaacag agacggaggc agggggaaca tgagcagaga ccttgtcatg
      841 cttttgttag ctgtttacaa tataagagat gcaagggggg caggaggggt ggtgaaagaa
      901 gaagcactta aaattaaaac aaaataaggg aaagaattaa gataaaggaa aagagaaaga
      961 cttgggaaag agtggggaaa cactgggaga atgaggacct tgttctccca agaggctcca
     1021 ttatggagca tttcttagtt catcagcaag aagaagaaag taaatgtgaa actgacagta
     1081 tgtgtttaac tgacattctt gaaaataata cataaaataa attaaaataa taagcctttt
     1141 tttttgtatt ctactttggc ctctttcttg acatttcttg caacacagat cacagatctg
     1201 ccacatgttc attagattaa cacacggctg atgtcaatta gcaaagacta tatcacatat
     1261 acttatacaa caatttagtg ctcattatta tgagcactaa atcgtacaga aatttgggtt
     1321 tg
//
LOCUS       KCVB01000015            1472 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 637pri ultra-conserved element locus
            uce-745, sequence.
ACCESSION   KCVB01000015
VERSION     KCVB01000015.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="637pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1472
                     /note="ultra-conserved element locus uce-745"
BASE COUNT          387 a          308 c          310 g          467 t
ORIGIN      
        1 taaacaaagt ctcctatagg ctgcgtcagt gtcttctcct ctgtcgaaca tcattctctc
       61 tttccccatt ataaaagaat gggaatttca acagctgagt gggccgagag atactcttag
      121 ctgcctactc agcatcaacc tttggtaaat actcagatta agaagatcca tgaatagtgc
      181 cggtatcttt tgctctttct gtcataaaga cgcctttaga ttcctcttaa tccccgtgtt
      241 aaaggtgcgc cagccgaatg gcttaagcaa tgttctgctt gtgactgaga gatgatgcca
      301 ttagacccgc aggattcgag atgccatgtt ttgtgcagga gtgttttaat gccacaatgt
      361 atcatcggac gtaattctat atagctcgcc tggcttcatt taattttaaa atatcaaatt
      421 gtttttctcc acagtcacct gatttcgcct gcccgtcgcc tgcgtcactt tcctccgtcg
      481 tctgtctaaa actggcactt ttgttgcatt ccaagaggtt gataaatcaa tccatcaaaa
      541 aattctttgc ggtgaaactc aatcaagaaa ggtcatgtcg gcgggaaaag aacagattaa
      601 attagcattg aattagacat ggaaagtgag ctctgtcaaa accatcactg tgctcagcac
      661 aattagaata tgttaattat gcatttcatt aaggggcctc ggcgctcttt gaatgtgaga
      721 tgaaatgtca ctggtgtagg cagccgaaaa atggaagatt gccaggcaca actttctttg
      781 tgcaatgtgt gacaccttca ttaacatcag ggattgctag tctgattaga ctttttgtat
      841 tagtttaagc ctttaggctt tcaaatgcta tgcctttttt ctgcgttttg atgtggcagc
      901 taagaccctt ctccatttcc aagctgatat tgtccatggt tcaatccttt gtttaaattc
      961 tccaacataa tgccgtgaca ttggacgtaa catctcattg tctttgtgta gatgacggca
     1021 tttactgtgt ctttgtttca cataatcttg ggtcactttt ggcgatgtga gacttgaaga
     1081 ggcaaattcc cacactgggt gctcgtgtca gggatattca gggtggcctt tgcattatag
     1141 catgtgacgt tgcaactgcg gatagtttta attttcagct ttagcttcaa aacatcagta
     1201 ttattacttt gattcaccta gaatagatag atggatagat agatagatga ggtggttact
     1261 tacggttcca tatgttcacg gctcttcatc atactagaga aactgcagga cagacttatt
     1321 tctctgcacc agtgattaac tcctgaatga actcgggaat ctcccccacg cggtgtcatg
     1381 gttgacagct catcatattg tattctgtgg caatcacacg gagaacagat gataggcaag
     1441 tgaaagttgg gccgcggctt acattacaga tc
//
LOCUS       KCVB01000016             917 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 672pri ultra-conserved element locus
            uce-853, sequence.
ACCESSION   KCVB01000016
VERSION     KCVB01000016.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="672pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>917
                     /note="ultra-conserved element locus uce-853"
BASE COUNT          272 a          200 c          168 g          277 t
ORIGIN      
        1 cgtctgtggc tccatcacac ttcaggaggc tggtgtaata ctgcaaaatg tcttccccca
       61 gacgccgcgg acatacgtaa atcacttcca cattttcatc taaaagtata caaagaggac
      121 ttcaaattgc atatttacca taaagcacac agagaaaatt aatgtgtgta cataatagtg
      181 aggatgagaa aaaattctac ctctaatatc acacagtctg cctatttgga tgttctgtaa
      241 gatgtctaat cccctcaagc tcagtcgctc gctctgagaa tatcctgtca aaagaagatc
      301 acaaatattt gtataagact ttcaaagaga gattcatatc ttctctaatt ggctttgttc
      361 aaaatctgta gctccacaat accaacctcc catgtacatg ctactagact tgggggtggg
      421 gtagttaagt ctaatcacac agtagtgcaa tctgatgatt aatttagctt aataattagc
      481 aggtgcagtg tctgtcgtgt cgtgtactgg gagtcctgtt gcaccatgtc cagctaccac
      541 tgagctacat ccagtggtac ctccaggatt ttactcaatg tctgtctgga tttctgcctg
      601 agagagaatc ctcaactgaa tcaaggtaat taaatgattg gattcatgct aattaacaac
      661 aatggggttc aactttctaa ttatccttgc tcacacagca ctctctgtag ccaccccaag
      721 tgcaaataaa gcattccatc tgctagtaat taggcttaat aattttagac aatcgctcaa
      781 tgatttacaa tgcacatgcc tgattacagt ctaaatgcca gtgccatggt gaaattagaa
      841 caagaacgga tcatagcttg cactctatgt ctagtggcaa aacgcagcag tttcttcctc
      901 ttttctgtgt ttttttt
//
LOCUS       KCVB01000017             958 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 708pri ultra-conserved element locus
            uce-14, sequence.
ACCESSION   KCVB01000017
VERSION     KCVB01000017.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="708pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>958
                     /note="ultra-conserved element locus uce-14"
BASE COUNT          237 a          183 c          209 g          329 t
ORIGIN      
        1 tgttccaatt aaaattgtaa aaaaagagga tgaaactgag cttggtggaa aattaaagct
       61 cggtgactcc ccggaccacg ggaccgctgt atatttgaaa ggtggctgga cgaaacacct
      121 tcatgcgtcg gagcatatga gggtgttttt gaccacctga tcttactgac tgattattag
      181 tgcagaccag acttgactgg accacggaca caaacgcatc caaacttctt ccgagacttc
      241 agtgtggaag tcctccaacg gttcagcacg atgatgatga tggatgatgg atgatggatg
      301 atgatgctga tgaagctgct gaagctgctg aagacagcct ctctgctctg ggaaactgaa
      361 tcaagtcaca tttgatttgt ggaccaataa gacttcagga agtcaaagta aagctgactc
      421 cgcactgaat cgcagcagtt cttccaactg ctccgcttag acgcctcact catgtgtata
      481 ttttaaaaag gaagaatttt agaatcgtct ttagtgtatc aaacccattt ctaacacttg
      541 cgtcaataaa catttggata tttaaagcac tcaactcctt atcccccctc ccgtctgttt
      601 gacccttaac ctgtttagtt gctttctgac atctgtgctt tgctttactc cttttctgtt
      661 ccgcatgtgt gtatattatc atggtataat ttattctgct gtatgaagta ttagagtagt
      721 gggaaacttg tactggtatt tactaccttc agcctgttgg tgtgtttact gtaacactgg
      781 cgtaactgtt atcaattaaa gatccaaaac tgattctgcc atttgtctct ttttttattg
      841 tgtgtgtgtg tttattggtt tccatatttt cttaatcata tttaatgttg atttcagttt
      901 gttttaccat gtgtgtttgt gtttgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgt
//
LOCUS       KCVB01000018            1207 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 751pri ultra-conserved element locus
            uce-51, sequence.
ACCESSION   KCVB01000018
VERSION     KCVB01000018.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="751pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1207
                     /note="ultra-conserved element locus uce-51"
BASE COUNT          378 a          242 c          284 g          303 t
ORIGIN      
        1 cactcgaata aatagatgtt ccaataataa ataaataaat aaataaatat gtgcaacgtt
       61 tgctataact tgttcaaatg tccaagtaag tagccttcat gtaaacagct gatattatgt
      121 gtctgcattc caggagcaaa tgatgcgtaa aaagcaggca cagcatctgg acgcccgcta
      181 cgtgacgatg gtggagaacg cctactatta ctgcaacccc ccacctgtgg agaagacggt
      241 gaagaagaag cggccgccgc tgcaggagta catccgcaag ttgctctaca aggacctgtc
      301 caaagtcacc actgagaagg tgctgaggca gatgcgcaag ctgccctggc aggaccccga
      361 ggtcaagagc tacctgatct gctgcatggt caacatctgg aacgtcaagt acaacagcat
      421 ccactgtgtg gccaacctgt tggccggcct ggtggcctac caggaggacg tgggcatcca
      481 tgtggtggac ggagtgctgg aggacatccg gctgggcatg gaggtaagga gagaaaactg
      541 tatggctgaa aatatgaact gacactggga tgtagtaata ctgacaatag gtaatgagtg
      601 aatggtgagg ttagatatag tgagaggacg ggaagaccaa caaaaccagt tgctaatctt
      661 aactgacata tttgacagtt gactgaaggg gaaatccatg ttgttactgt cagttgtgta
      721 aagaacactg caacatgttg caggacatgt gagaatgctg cctggctgcg tcctgaggtg
      781 aagtcgtggt tttgcacaac acttcaaacc atttcatgtg gttgaatcta caaatgagtc
      841 tctgaacaca gtgccaggtt ataaaatgtt gaccagcgca ctttggaaac cactgtgact
      901 gtttgtatca gatttacctc tatctgatcc caaatcaata catggccagg gtttgtagtt
      961 ttcctattat ttttgaactg acactggaaa ctgcaatatt caaaataagc aggagaattc
     1021 aaaatccata gtcaggagtc actttgcaaa ctaaaatttg atcactttgt tgaaattgat
     1081 acgtctgcac agatgaaact acccagcagt ttattaacta gtaaaaatta gctccaccag
     1141 ctaaaacatt aaaatgcttt ttacatgttt atgcgtcggt aatagtaatc caataatata
     1201 ataaaaa
//
LOCUS       KCVB01000019            1067 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 792pri ultra-conserved element locus
            uce-816, sequence.
ACCESSION   KCVB01000019
VERSION     KCVB01000019.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="792pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1067
                     /note="ultra-conserved element locus uce-816"
BASE COUNT          322 a          270 c          218 g          257 t
ORIGIN      
        1 acacacacac acacacacac acatacacat acacatacac acacaccaca cacacacaca
       61 gacacatgaa ggggaaaaaa aacaacgcaa atacagtaaa aatgccccca ctttacagta
      121 agtgagagat caataatgat ggatgaggga cttttataca atcggcatgt tctcctcagt
      181 tcacagccat atgacaccac gcctgtggct atcccattct gcacttcagc agtcaatcac
      241 atcccagctt taaaaataac agtttgttag caccattgat ttagcactta gagcgtgtcc
      301 ttattacctt gtaattatgg tcagtgttga cataacagca cagacaacca ggcctcccac
      361 cctctaagag aatttatggg ccctggtttt attacggcca tttcatgtgt ctgggtcccc
      421 agttaagcga cagacttgag ttctagccaa gggcccgtat ttcactcatc gaggtcaggg
      481 ttctcaggaa tcccccgtaa aactgaacgc cgacgggggc ctgcgtgtac acacaggaga
      541 cgcagggaag ccctccccaa gggctcatta aaataaatag ccgattacaa gctataatcc
      601 aaataatact attcatgagg gaaatgtgat gcttttagga cgcaaaatgg gacacgggtg
      661 gagtcgtgtc gtcacataaa accaaacact gcggaagccc agcagagatc acctcttaat
      721 aatttacaac tagaccctcg ctttaaatcc attagaggat agtcagagta cgctctccta
      781 ctctgtcact atgttatgac ttcagcatac ggcttgacaa aacaagagta atctgtgtca
      841 ttaagattgt gagatactac aaccgagcct tgcttccaac ccggactgag tttgcttttc
      901 aggtcttcag tgaagcagaa atctgacccg gacagtgaaa agtaatacgc cagcaggact
      961 gaggctgtct gtggcggatc tttagcacag agccgggtcc cctttgtttc cccaagtacg
     1021 ctgcctttgt catctttatg tgaaaaatgg acctggctgt tttgaca
//
LOCUS       KCVB01000020            1327 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 836pri ultra-conserved element locus
            uce-1022, sequence.
ACCESSION   KCVB01000020
VERSION     KCVB01000020.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="836pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1327
                     /note="ultra-conserved element locus uce-1022"
BASE COUNT          342 a          224 c          291 g          470 t
ORIGIN      
        1 caattttaat taaagaaaca cacttaggaa cttaataaat aatgtgtaca cgtgaaatgg
       61 ttatttaatt tcaatgtacg ccatagtttc tcaaatagta tatccattgt gtgcagcata
      121 ttttttataa gccctgcgta gatttgagca tgcacgcaca tatatacaca cacatatttt
      181 tttatgtacg tattattctg catcttccat aagtttgttt gacaaaagtt ttggaggttt
      241 ttttttactt ttttgtatgg gttttcgtcc cacttatatg attagatttt cttcacgcta
      301 atttgtgtac agtcagtgtt gagaagagag aaaaggtgtt tatttaatcc acacatttct
      361 cacactatga atcaaacaca tttttttttt taaattagcg cttggggtgt atagacgggt
      421 gggtggaatg ggggatttaa catgaaaagt tatcattaaa gttgagtctt ccctgtttgc
      481 atggtttggt ggtgtgaggg ccagggttta ctgtcatggc aactttgcgt tatctgatgt
      541 ctctgagcag agaaacattg agggagataa tccctgaatt aaacaggatc tgctttgagt
      601 cgactgagat gagcagggcc aggctgtgcc gacgatcccc gccgctccca tgctgcgtga
      661 acaatgggaa ggcatcctgt ctctctgtgg ggcagggctg aacttctctg ggtttcattt
      721 gcaattgaat tcttggccct gatgaaagtg ttgagggggc cagagccaca atgaaaggga
      781 ttcatagaga aatgcaattt gtaaatcaac ttgtatgtta aacagcaaga ttttagtgtg
      841 acaaagaggg aaaagaataa ggaaagtctg gagcacagca gtggctctgg gggcagggtg
      901 tcgccttgcc ttttcactcc ctgactcgtc ctgtttatca aaatgtaaaa ttaaaaaaac
      961 tgtttcgctt ttcaaaggta gtcgtttctt ctaaattttc atctcaatga caacagacgt
     1021 cgctgtattt gtgttgcatg ttctctgagc tggtgttccc agtttctcag ttttggcaag
     1081 tttgtgatgc atgcattttt atgtgccacc ctactcttaa gttttgacag tcatttattt
     1141 aaacactttg cattccatgg cttttttccc cccagaagaa tttactaatg agtttctatg
     1201 gctggtaatc ttttttgggt cagagtttat tcacagttat ggggaaaaag tctgttgact
     1261 tttgaaaatt tctctgtttt gttttgtttt gttttgttgt ttttcttgag atggcagaca
     1321 tttaact
//
LOCUS       KCVB01000021            1332 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 871pri ultra-conserved element locus
            uce-311, sequence.
ACCESSION   KCVB01000021
VERSION     KCVB01000021.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="871pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1332
                     /note="ultra-conserved element locus uce-311"
BASE COUNT          326 a          339 c          316 g          351 t
ORIGIN      
        1 tttctgcttc tcattgagga atgtttgcac taattgcctg ttcattatta aaggaaatgt
       61 tattttacct aaagcaataa aattgtccat taaaatgcca tccttgggcc aatatgagct
      121 ggtacatcga gggtaaacat gtgcatgaat gtaattagat atgaggaaac attaaagtac
      181 agcattagag acacttcgac tgtgttcaca ccactatgta tggggttatt aatagttgct
      241 atgataccca catcccctca tctcacttct ttgacagagc ctcctgtgat ttaggccacg
      301 atataaaccc tactgggcca gccctctgtc cttttctggt tgctgttatt gttgcggagc
      361 acaggccacg gtgtttggct gctccagctg agaagagagc acctactggg cttttcccat
      421 gatgatgaga accgctctgc tcggcccagt cagcaggcag tggataaggg cccccgagct
      481 gcttccacct ccactctctc cccttccctt aggcccccca agcctccacc tcctccccct
      541 tctccctccc tccctacctc cctccctcct cctttaagtt ccctcaagtc atgatctagc
      601 ccgtaaatta gtactgggag aaaaaatagg ttctgtaaga tgaaactggg tttctgtgtg
      661 tatcagcaca cgactctggg gagacaagag agaagaagca ggcagtaaat ccattggagt
      721 ccactgatag cgcctgtttg cacagccact gccgcttcct ttgatcaaat ggctggggga
      781 gcggggggtc gtgtggcgct gccgaaggtc agctatatat tacactccta aaagtgatta
      841 ctgaaattct taagtgaaac cattctgctg ccagggtgtg ctctaatccc ctgcagcgtt
      901 gtctgggccg gagccggggc cggggcctct ctgtggatct gtgcgcccag cactcctttt
      961 ccccttcccc acttgtgttt tctatcatca gagcaggcct aaaaagagcc ccctacatgg
     1021 aaacatggcc tatccagttg caatatcaac cactggggct tgcaggaaag agagagagag
     1081 agagagagag aggaaaggta gtgtcaggaa acagaaatat gcgcacaatt caaaggctgg
     1141 ggcagcaaat taagggttag gcattggaca cgacagtaag tggcctttac atcagaaaca
     1201 gggtaaacaa tgtctggatt gtgtttcgct tctttcctct gtgttgttgc atagagaccc
     1261 cccggctctc catctgcaca ctggctgtgc tgtaggtaga gcactaaaac agctcaatgg
     1321 aggttgtttt tt
//
LOCUS       KCVB01000022            1574 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 913pri ultra-conserved element locus
            uce-1323, sequence.
ACCESSION   KCVB01000022
VERSION     KCVB01000022.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="913pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1574
                     /note="ultra-conserved element locus uce-1323"
BASE COUNT          564 a          277 c          285 g          448 t
ORIGIN      
        1 caaggcatat gaagcatctc ttcatagact tttaaatgat atacagtact gtcttgtaag
       61 cagaaaagat tacagctctc tgcattattc atgtcttcat gcattgccaa atctatgaca
      121 cataaggtaa taaagattac tacctgttat cattgaagtg tttagcacac tgttagaatt
      181 gtgttccaga tttatgttaa tggtacagct ctgcaaccaa ttagaatgca ttcacttcgg
      241 tctgaagtgt acacaaatca tctgacaagt gagtggtcat tcaaccttga ctattgctgt
      301 gtgtgtagat cacacagcac ataaacataa gtcaatctga tgtgctgctt aaatcataca
      361 gagtgagtta gagggattat aagccgactg aaaatgatca aatttagcat tttgctgcaa
      421 tatacacaac taagctttat ctagctaagt tcttttctgt caggacaaag tccaggacag
      481 cctgggaaat aggtaattgg aaagaaaaag agactgcact tctgcaaaat ctagtactgt
      541 agcttaattc cttaaccaaa tgatgcagca aaaaaagaga caagaaagaa acatagaccc
      601 agaacgcaac tctacataaa caaaacaaaa caaaacaaaa caatgaaaac agttgcataa
      661 gaaaaataaa agacttactc aaagacagtt tgttagtgtt gtcctttcct ggtaataatt
      721 ttacccctct ggatttaagc tctccagagc ttttcactgg tcgagagcaa tagaaagaat
      781 tcattagata aaagaagtta cagtattaga tgtgagaaga tacagtacaa atgtttcttt
      841 ctctaaagac ttggtcctat gaagagcatc aaaatgtaac tgctactggt gtgcatcctt
      901 tttttaaaac aaatgagtcc aacggccctg atgcattcca aagggttgcc ctgacaattc
      961 cataaaacct acattaattt ctgatccttc tttgtttgtt cacataactc agacataata
     1021 aacagtggat ggaaattgat tggtgagagc agatttaatg gttatagctt gtaggagaaa
     1081 gaaccatgca gaataaagga gcagcctaag gactgcaatg ctacatctta agattaatta
     1141 aacacagcag gggccagatt gcacacaaaa gccaggtgat gcaaacggct gtcataattt
     1201 tgaagcatca acagaacagc caaaagcaat gaatgatgta tcaagcaaaa acaatgctat
     1261 ttgaatttga aaacattgga atgatgcctc tccttcaaac tatgttgtgg gtcaaatggt
     1321 aaatttaatc tcatgtaaac acaataagga tgtgaatcta ataagttatt ataattttta
     1381 tttagtgacg aggtggggta aggataactc tatgttcaaa agcatattgt gaaatataac
     1441 ccctccgtgc ctgcctttgc ttaaaaatat ctgtcctttc catcataaat aacatgcata
     1501 ttaaagtggt tttaccactg cacaacaatg aactgcaata gatgagaaaa gcaatgcaat
     1561 tgatttgatg tcta
//
LOCUS       KCVB01000023             975 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 956pri ultra-conserved element locus
            uce-479, sequence.
ACCESSION   KCVB01000023
VERSION     KCVB01000023.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="956pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>975
                     /note="ultra-conserved element locus uce-479"
BASE COUNT          248 a          257 c          182 g          288 t
ORIGIN      
        1 cacacacaca cacacacaca cacacacaca cacacacaca cacacacact cacacagcca
       61 cgcaagagac agaaaacaca gctctgcgat tgcgcacaag acgattgggg catttttaat
      121 caagcacagg ccatcacatg ttatgaacca gcctatttgg taggcagcct tgatgcttat
      181 acacttccaa acgccaggag ttttagtgct gattcctatt gagcctgtta cagccctgta
      241 atgaatcctg tcgctcgtct ggcctggctc agattcctcc tccacatacc agggagatgc
      301 caggggccac acagacgcca gaggagcctt cctaaatcac agccttgtta cactgatgaa
      361 tgtgtgtgtc ctgttctgtg atctaggcta tctgcctctg tgtgattgcg tgtgcacctc
      421 tgcttctctg catgttttct ttgttcgcat gtctctctct gcctccgaat gtgctgctag
      481 ttatgtggtt atacttgaaa atgagattag agatgaatgt gaagggaaaa gtgaaattag
      541 tgtcttttct atgcaggtac agtttcctat ggtgtatatt cctctacact gggtgggcta
      601 caagctgaag tcaatagtca caatcctgat cctgtggtaa ctataatagg cctactgtca
      661 actccaccga gagttgacaa ttttctatcc cctgtctact ccattcatac ataattcata
      721 ctaccatcaa taattcacca tggtgacatt accaataata acacaacaat actttgtttt
      781 cgctgtgtgg tttaaaatgt gttgccttgg taacagagat gaaacaaata ttcccaagag
      841 gagtgatggg aacacatgaa caggacagaa ttccctcctg atcccactta gtagtgttgt
      901 cacactctct ttctttctct ccctcctgct ctgcctctct ctctctctgt cttctctctc
      961 ctttctttct ctttc
//
LOCUS       KCVB01000024            2170 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1000pri ultra-conserved element locus
            uce-1187, sequence.
ACCESSION   KCVB01000024
VERSION     KCVB01000024.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1000pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2170
                     /note="ultra-conserved element locus uce-1187"
BASE COUNT          538 a          509 c          469 g          654 t
ORIGIN      
        1 ctattttatg aggctctaat aagcagataa caggatggag acaaaaacgg atgtccagtg
       61 aggtgcagac tccaccttct ccttgtttga ctgaaattaa ttagatcctc ctttgaggtc
      121 acggctttgc accagccacc ctttatgcct ttgatttttg tgccattaat gggttgttat
      181 agtatatttc ttgtttaatc ttctctctca cactaagagt ctttgtagac gagcctaaat
      241 gatttcctgt catgatgatg ctgttttctt ttgcaaatcc ttttgacctg tcactcattc
      301 cagcagcttt tttcttcccg tatgtgtctg aatggatgcg cagacgtgtt ttaaccgagt
      361 atgaccctat gcacagcgtc ccatgtgatg ttgctgccac tctgtcccac acgtgcttcc
      421 aggtggctga tcggtgtgtt tcggtgagta gcagcagtgg cgcccacgcc tgcgcactgc
      481 ttagagaaag tcctcctttg ctgtccatgg tgtggtgagt aattcagcca taatccgcct
      541 ttctcctctc ccctcttctt tcccctctcc tctccccctc tcctgtggtt ccattcatat
      601 tctaatcaca gctttccctt ctccagctct caggcactcc atcagagccc tcccagcctc
      661 gggctttgca cttttacttc cccccctttg tgaccattcc ctcttctcag aggcacacca
      721 aaaataaggt tgccacaacg aaaaagaggc tgacaactcc accagaaaat tcacagtggg
      781 gcagtttttt agcatgttca gatgcgtgtc cgagcccgtg atgtgtcgac tctgtgaaaa
      841 agttgccaca aagatggttt aatggtggaa tgaggtgtga aacggaccaa atgtggcaac
      901 aagtgtccgt gggatgtgaa ataggagtta aaatcagaag gaaaggcagg agcggcggtt
      961 gtgtagggat gtatgtttat atggagctct ggggtgtata aaaacgtatt aaatgccata
     1021 atggaagacg gggaggccac agtgttagga ctgcgtagat ctaagcctct tttttttcgt
     1081 tgggagggag cttttcttat tcaaaacagc cccacaaaag acttccagtg agccttgcca
     1141 catccccatc tgacttgggg ccattcatca gccttccaag cctatcaatg acatgtcccc
     1201 atcagggctc ctataaaatc agcccctttc ccatgaaaca tcagcaaaca ttttgacggg
     1261 tctggctttc cccccgctgg atttctggag tctctacccc ccccttcctc ccttattccc
     1321 acctcccccc tctcctccgc caacaccacc cctctctggg cgaacctcag atcctcaaca
     1381 tctcctgtga cgggcagtgg tcacggaaac acagagcaag acaaggggag agccgctgag
     1441 gagggggaaa cgtgcaccaa gccattttct tggaggagcc agtgtcgttg atgggaagag
     1501 gatgcattgc gggctgctgg aggagcctga tatggattcc acaggtcagt cctgttaaac
     1561 tctgctcgct ttgtgtggat tgtgttgaga aaagtagctt ctgttcagaa acgctgtggg
     1621 agctgctgct tcagctgctc ctatggaagt ctcaaacttt ctctgaagtc attcagagga
     1681 cgtgtctcaa ggttcggctg ctgattttta ccttttttat cacttattta attttttgag
     1741 ggtatttatg aatgagtgtg atcgatgaca cacctttatg agaagtgctg ttgttgttgt
     1801 aagacatagt tgtcggctga ttgaagaaaa gtcacaaaaa acaggaaaaa aaaccttaaa
     1861 tttgccatta ttggttcaat gtctttttag tcaggtcagt ttaataaaac ctgcattcat
     1921 cagtggagaa atagaaacag caagtgcaga tcaaagtgca cttcaaatta ttatgcacct
     1981 gctcttttca acttattttc tgttatgctt cctagttcaa atttgaaaaa actaaagcct
     2041 ttattcaact tgaaaactga attttactga gaatttatag gtaaaatatt ttgtatttaa
     2101 tctggcctta attctttatt tggttttcag aaacataagt gattaatgta aaatctgatc
     2161 aaaagaattt
//
LOCUS       KCVB01000025            1609 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1040pri ultra-conserved element locus
            uce-698, sequence.
ACCESSION   KCVB01000025
VERSION     KCVB01000025.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1040pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1609
                     /note="ultra-conserved element locus uce-698"
BASE COUNT          440 a          309 c          361 g          499 t
ORIGIN      
        1 tggtaccaac ggtatacaaa gacataactg tgatcatcac attaagacct tcagtcaaca
       61 gtgtagccat ctatgtcaga tcgtgccaat gacagtcatt attttgagat ttgttatgaa
      121 caaaatgaat taccatcgac aaagttgaag gtaacagagt tgaataatct tgaaaatgtg
      181 agtttatttt taaagctctg ttttaaccat cttactggtg ataatttacc actctaggaa
      241 gcgtttaaaa gtgagatgta ccagtttagt gtaggtggga ggccaaaatg cggagatgat
      301 gcattttaag aatctgcact ggtgttgaca gggaggaaac agtcacacag ggtgagactg
      361 tttgtttttt ggtgtggttg gtataggccg cactggacgg gctatatgtg tgtgttgtga
      421 ggggtcgttg acagtggggg aaaacccttt tagcgcgttc ataaaagcgg catttagcat
      481 tgtcacaaca actgcttagg aaaagccaag cctccattaa acaagggtaa tgatgcacta
      541 caaagtcaca acccctcagc atagcagccc gccatccagc cagcaaaccc ctctgcataa
      601 cttactgcct gtgttgtata cagttcctgg tacacagcag catgagagag tttttctgag
      661 tgatttccac tgtgaagtta tggtgttgat tagcgcagtg acaggtgcca tgattaaaat
      721 ggccgatagt aatagtgcag cattcatgca ttgacaactt tgctctgcat ggctttgtga
      781 ggggtgatgt atgacaccat tagggtaacc tcagggccct ggaggacact tatctccctc
      841 gtcacgtcct tcacacttcc cattctccct gcaacaaaag gacgcctgct ccccagtctg
      901 catgcttatg aattgtgtgt gtgtgtgcgc gatgtgtgag tgctcgctgt ccatctctgg
      961 tagtgaacat gaatactggt ttatgtgtag catgttctat tatgtctctg tgtatgtttc
     1021 ggttcgtaca tctatgtcag gtttagactt ttttatatga caatggaggt gattatactg
     1081 tactgggtga catcattcta tgcctttact gtacaaaata cttggtactt tctcgttaac
     1141 actgagcgta agattatgcg ctgtctctca cttagtttgc taagcattta aatataacat
     1201 aaattttaaa atcccccttc ttttctgcat ccgtaatttt ttactgaaac acatacaaca
     1261 agaagtattc aatcaagttc acgagagctt gtacattaat ttcatgtact gtccacactt
     1321 tttattcaac tgtaggtatt agtggtacag tagttctaaa aaaacggggc actgaagatg
     1381 atggtgctgt ttgtagcatg gcaagtgatc catgggaagt gaattgcaca atcataaaaa
     1441 tgattgtggc tgtgataact tcgacaactg taaaatcctc tgtcatagtg ttataaacaa
     1501 ctctgcagtg tttatgtgat tgtgtttcat catctcactg gttcctgaaa caacgtggct
     1561 gtgtgtgttt tcaatccaag cagctggcaa gggacaacag acagttttg
//
LOCUS       KCVB01000026            1063 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1084pri ultra-conserved element locus
            uce-249, sequence.
ACCESSION   KCVB01000026
VERSION     KCVB01000026.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1084pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1063
                     /note="ultra-conserved element locus uce-249"
BASE COUNT          381 a          184 c          246 g          252 t
ORIGIN      
        1 aataggagga cccccctata tttcggtgaa gcccatcatg accacctgat tgcattattc
       61 acaaatggtc ctcaggaatg aatgtaattg tgtctcatta agggccatga agtgtgacag
      121 gaagcgactg aaaatgtttg agttttacga aaagagaaca agaaaatgcc tttaaaagca
      181 acatatgtag gacagtctgg agccaaagtc atatataaag acaaagacat aacagaagca
      241 tagtttttgt aggagaagat aacaagcctg cagactagga tgaaactact gtgcatgttt
      301 ttgatttagc agtacagtaa tgccttcatg taaccgcaaa actccgtaca tggcatttaa
      361 tgaaatactt tcacaagaac aagtgtccag aaaaaaaaaa aaagacaaaa agacatccca
      421 ctcagtcctg cttgcctcca cctctgcctg tttatgatga agggctcttt ttggtgtgag
      481 agtgaaagag acaggctggg ctggctgtca tgtcataaag gatgcagtca gagagagcag
      541 acatgatttc atggctaatg attcccgcac taatatcagt gtgggctgca gaggggataa
      601 ccaagttgag ctgtcttagc tcaaatgatt caattaggcc agctgtccct atttgtcatg
      661 gctcaactgg cttctagatt acctttccag agtgcatacg tgcctctgca ttcaacttac
      721 cagctcaaca gttttcagaa aaaaagacaa aaaaaaggga aaatggagag caaatagggg
      781 aaaggaagaa aggagcagtg aaggagaaga aataaaacaa agaatgccgt aataaccaca
      841 ttatccactt aaacgctgaa gccaattaca gagataaata tgactttttg ttttgtggag
      901 gatctttggg gtgaaatcag aaaagagcct cagagcgtgc aggagtgact ggagtgatgc
      961 tgctcgtgaa aatctgatca gtgaaagcat gagagacaac atgaaggtca aaagagaaaa
     1021 gagagaaagc aaaaaattat tagtttatta aagaagaggt taa
//
LOCUS       KCVB01000027            1866 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1122pri ultra-conserved element locus
            uce-564, sequence.
ACCESSION   KCVB01000027
VERSION     KCVB01000027.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1122pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1866
                     /note="ultra-conserved element locus uce-564"
BASE COUNT          561 a          401 c          378 g          526 t
ORIGIN      
        1 ctccatgcac tcctctagcc gtgggtcctt ggaggcggag gggccctatt gagcggggcc
       61 cgggctctcc actagccagg ctctttattg agccgtctat ggggctttat gttgccagcg
      121 ctcggccttg ccctcgccat catcaatcat cctcggctcc agggctgcta tcactgccca
      181 gacaggcggc caatcacagc cgtccaggaa gctctatagg agatggagag agaagcagag
      241 aagagaaaaa ctagagattc actctgtttc tgttgctgcc agtctcaagt ctgatttgat
      301 tgacatcttc tgtacctcct gggcgcatga caacactggt attaaccaag actggcctgt
      361 gtgaaatttg attagatcat gaagtgagag tctgagtcat aaaagcacat ataaccctaa
      421 cacaataatt attatagtag gcatctggct gcaataacaa agcctttcat gagagatcta
      481 tattaatcct ataagtttga attttagatg aagggttcag cgtacaagaa ttgcagcaaa
      541 actataaatc cccaaaggaa taaaggaata atccgacaga ttaaacccta tcaggttact
      601 gactcactca cgccctgcta gaagtttttg ttggaataat gtagttttat ttaagtcaaa
      661 ctacaaagag gaatttctat attaaagctc actttcctct ttttcatcaa gcctgaacag
      721 tatgtcggat tcctactcaa ggcgtaactt gattctgtgt ttcttttctt tacttttctt
      781 ctcttttttt accccatctc tccctgcagc cctgagctct catcctgctc caggaggaat
      841 aggcgaaatc tgattacaga cctattttac agcagacgcc tgggcgcaat aaaaggagag
      901 agagcacgga aacgggactc ggcccatcaa ttagatctct attagcagcc aatgcagacc
      961 tcacctcagg aaacgccggg gccctgaaat taaacaattc ttcaagcccg acaagccagt
     1021 caactctgct atatgtttga gaaagctggg ggggggggtc tccctaccag agcaaactat
     1081 ccaatacctg ttataaaatc attaaaaata agcattatgg actgtattga atagcttaaa
     1141 agaaagaaga cacataaaga cataacctat aagataaggt cgcttctgag tactgaatac
     1201 tggcagtaaa tccttattat aatagtaaaa atgttccatt gttgtaatat tacatatttc
     1261 aataaccctg atttacaata aggtaaaaat atcagcattg gttatcacag acacagtaaa
     1321 tgtgactgac tataaaaccc aggatatcat aacaaagtac aataatggca ctaacattga
     1381 caaagtaatg cattatagca atattatagc aatgatacag tagcttatct gtgtcatata
     1441 acctccttcc agtgtcatta atctccaact gttgacctgc tgacctgagg cgggacaaca
     1501 tgtcaatatt ggataattag cattcagcat taaggccatt tagacaaggc actttgagag
     1561 gaatccgtgt ggtcattgat tcataatttc tatttcagaa aattaaaaaa tttttgccca
     1621 ctttgtagtt tttctggttt catctcaggt ctgctcaaac cagagctgct gcgagataat
     1681 ggggttttag tcaacaggat gtggtacttc catgttaaca ggggtttact ggtgggaaat
     1741 cgtgcaggga tactggatca gcttcctgta gttccaggcc agactcccat atatttatgg
     1801 gtccttgtct ggtttgttac atccagtaac ctcataagaa aaaacaatgt tactgggatg
     1861 acatca
//
LOCUS       KCVB01000028            1783 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1167pri ultra-conserved element locus
            uce-521, sequence.
ACCESSION   KCVB01000028
VERSION     KCVB01000028.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1167pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1783
                     /note="ultra-conserved element locus uce-521"
BASE COUNT          504 a          396 c          371 g          512 t
ORIGIN      
        1 gactgtttct tgtgtgtcca gaggaaggcc tgcagaccaa aacatcactg ataactcaat
       61 tttcatgaac ccttcttggg aggctgcgcc gtttagcaga tttaggtcta tgtttcatgc
      121 acatgtgcac cctatcagat aggagatact ggaaggcatg ctcctgcctt gtgactgact
      181 ctggtatttt tggaggtgat gaaggccatt atcagtggat taacagaaac taggatggtg
      241 ggggctcaca gtgttggaac atactgccct aattctccct cgcctgtatc ccatgtatta
      301 cccttgtcga ctggccagca gataagatcc cttggagaga cggaagctat gcaactagtc
      361 gctgcacctc tcatatgacc ataatgccag agccaaaagt aaaaggcgag tccagtaatc
      421 cgcgtgaagc cgagtctgct ccaaagcaca gacagctttt cagtaaaaaa agaggaaacc
      481 tgggctctga atcctctgga ggcacataaa gtagcattca acatgtcaca acacagccaa
      541 aaaaacaact ggtttctgct ccctggaaac acacacacaa cagctgcatt catacgcacc
      601 caccattcat cctgattcat ggcataatac aattggacag gtggattgat aatctaatat
      661 ggcttagttt ttttatccta ttcctctcac ttttcaaata gtttacttgg agcccgaggg
      721 tgtgagccat cctgttttct atggccgccg aattacctca gagcatgcag tgcctagctc
      781 gctgccagag agggaggcga gtattgtttt catgacagag atcaatgggt gggtgaatgt
      841 gctttaattc tgcaggagct cctctcccag gctaataaat gcaagcctcc ctgctgactg
      901 ggctgtcgtg ctgagcccag cgggggtcag cctttttgtg ttcctgtgcc caaattatgg
      961 aaccatcaat acgctccctg gaaccagtca atagaagccc gctttgtggc cgaggggaga
     1021 ccaacttcaa ggctaatcaa aaaaagaaaa aaaaacctaa gctctgacac atttaccaaa
     1081 taagatcagg atcagaacat agagatgaat tatacattca aaaattcaga atggctcgaa
     1141 ttacttgtgc aacagatatt gggtgtgtct ctgcaatggg atgcatccag caaaacctgt
     1201 tttttttctc caaacacttt gtaaaaaatt atttacatgt tgtaaattct ttagcagtgt
     1261 gaggcgggag gttcatggtg tcagtgcact gtaattgtgg tttgaacatg gagctgcagc
     1321 cacttcaaaa tcatttcagt tgtttaggag ttatgcatgt ggcaataaca ttgcttaaaa
     1381 attatcgaaa catttaagct aaatgttaaa ttttgcaaac acagttaatg aaatccattt
     1441 tggaatataa gtttgctttt gaggatcact aaagcagaac tttttaacaa aatactacat
     1501 ttgtgcagcc tgaactaagg gttgtgacac actttctttt gacaatgcat gggcgatgaa
     1561 cactgttgtc atgcatgagt gtgttttcat actgccttac tgctgcttgt agcatcaagt
     1621 gaattattga gtatccagct tcaaaaagct tgtaaaactc tgtcctcctc aaaatcccaa
     1681 acttttttct ttccctcttt ccttttctct ccctcttccc cccttaaaat tcctgctgta
     1741 tccagactta aaagttgaag caagtatttg tcacctctat cta
//
LOCUS       KCVB01000029            1557 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1211pri ultra-conserved element locus
            uce-1286, sequence.
ACCESSION   KCVB01000029
VERSION     KCVB01000029.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1211pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1557
                     /note="ultra-conserved element locus uce-1286"
BASE COUNT          416 a          335 c          315 g          491 t
ORIGIN      
        1 acactcacac tggataacaa acattctcat ttggcttgca gacatacata tcattttggt
       61 agagttctgt ttattcagcg gcaaggagat gataaacaac atgacattgt ttttggtttt
      121 ggaagactat tttcagtgtt caccatgttt tttcctgtag gcctgtctct tgctttcatt
      181 gtcttttttt ggctttcatt catttactgc atattgctgt aagtttgttt ttaaggctgc
      241 agcttacggt taatttcatt ttagagcagt ctgataatat tttggattta caaatgaatt
      301 gtttagtcta tgatttgtaa gaaagacatt ggttgcaaat ttaaattgct ttttctcaaa
      361 gaacaaatat tttacattaa agcttactga aaaaaacaat tgatgatcag aattgttaat
      421 tttctagtct acttatagtt tcaatactat gtatgtatac atacctgtat tcagtctatt
      481 ggacactctg ctgcaggaat gcatgctcct aatcaggcca tttagacaat gaggcacttg
      541 tccaataaaa caatgcattt aaccctccac tgccaagtct aggtggccct gtcttctcct
      601 catctttctg cctcttccca ccagatagaa gtgtaatatt gtgccatgct tttaattgca
      661 gaaatacatc cttcccctca ttatgggcag caaagaggac aagaagcatc tgcagaggat
      721 ggagagcaac attgcggcaa tgagtggcac gctgacacag acaggtgaag attaatatct
      781 tcatcacttt ctccctcctt aacattttcc ctcatttatc tgtcgccaaa atcggggggc
      841 cccgcacgtt tttaacaaat gacgccgctg tcatttcgct ttaatttgaa attgcttgtt
      901 tactgtcagg ctcgggctca attaattacg tttcgctgaa aagctcttaa cctcggaata
      961 ttaatgagga gttgggaaat gacaactttt cttccccccg cccatcctct ctcactcccc
     1021 ccctccatcc gtctccccct gcaccaacct caccaccatc caaacctcca ccaccatcat
     1081 catcatccac cctcttcctc ataggtggcg agaaagttca ttttaatttt ctgtgtgtgt
     1141 atgtgtattt gtgcgtgtgt gtgcaggatg tgcgtgcggt atggcactgt gtggcgtctg
     1201 ggctcagcac tggccctagt gtgattaagt gcacggtgac aggtgcggct cccatgttgg
     1261 acaggtgagg aatctcaacc tggctgtcag gcatggagag agagaaagaa gcagggagag
     1321 agatgctttc cttgctcttt gtcagtataa ttaaaaatcc agggaggcgt aaatgagaga
     1381 gacactctga gaaagtttgg tctcgttaac agtagtgtcg aactggagtg tgttttcacc
     1441 tgaaagctac caatccctat tacagattgg tggccttgca gaaatgtaga cacagtggaa
     1501 actacagttt ttaaagaata aaaaaaatac attttacagt ggaaacggcc tatagga
//
LOCUS       KCVB01000030            1873 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1251pri ultra-conserved element locus
            uce-148, sequence.
ACCESSION   KCVB01000030
VERSION     KCVB01000030.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1251pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1873
                     /note="ultra-conserved element locus uce-148"
BASE COUNT          495 a          402 c          411 g          565 t
ORIGIN      
        1 attttcataa caccgttcag aagtatgcct ttcagtgtgt gttgaaacag actgtatagt
       61 gcttagaatc agaagatgtt aaaacagatg atgaaccatt ctaatgctgt agcaatagaa
      121 agcaaaagtt gtcagttttc aggatgagaa gctgttggag gaaacagaaa tatctaacac
      181 tacaaactct gactacacca gggagcagat gctgctctta aggaatacta cagcattctt
      241 gtatcttgaa agtggtatac acacctttcc atgctggtct gtgcttgtta gggactctcc
      301 agggttttca taaattgctt ttaagctttt ccaaagttgt tcttgtcttg taggttaaag
      361 actgactttg taagccagtg tttagtcaca ggacgaaagg attctttata atctgtttag
      421 cttcaataaa atcagagagc cacaggggga ggctgagact tctacatgtg cctacatccc
      481 tacctagcag tccagctgtc tgtgtccact acatctgtcc gcttagccag ttgagcccag
      541 tttattttcc tcagtaactt tccatctccc aaccatattt gtcattgttg tgtaattatc
      601 ttctgtctct ttgcgctggt ccctgctgta tatctcttat ctggctacag tatctttttc
      661 tctgcttgac tctcttccac ccttgtatca ttctgcctgc gattcagcct tctttttcct
      721 tgccgtgcct gtgtctctgt catctgtcca gtctcagctc cctctgagtt aaacaggctg
      781 aacaaataga ttacaaacag tgggtagagt gaagctaagg aggaagacaa tctgaatttg
      841 ttgtggatat tgaatgagga aggaggctgt gcagtcaaag aaagccagtg gagtcagagt
      901 aaggctgtat ttgcctgagg ggaaaaggct gaaaagtagc tctcgcaaca gatgtgcggg
      961 gcagtaaagt gtaattgaaa tgccagcagc aatggtctta tctccctgct acaggagcct
     1021 gctgccagtc tcaacatcag ttagatgtca ttttacctaa gtctgaatca gcttgccaca
     1081 atccgcaatc cccattcctc tgatgctagg caggagaaaa agggagaaat ggatgagggg
     1141 gagggaaagg agaaagaaaa ggagggtggg gagtcagtaa taggatttca gtgcacccag
     1201 ttaacttcca ccacctggac ttagttggta atttgatatg ccccatcaat aggattcatt
     1261 tacctcatcg cagtattacc atcgccatta atttgacttc ttaaaaaata tatttattca
     1321 tttctctctc cacattttcc tactcctcct gatcctaatc ttgcatactg gaggtgggag
     1381 gaaggaggaa ctgttccaca atattttcgc acagaaagaa agtccttggt ccagaggagg
     1441 tggaaagcca gacaccacgg ctgtcctctg cgcttcaggc aaagcttaat gttttgggcc
     1501 aggcagcatc cctttgggga gaaccgtaat gtcctcccag attagaggct ggcactcctc
     1561 agagctgctg ccttttatca ggaacttttt taatgcaaca tgggatcatc tgcgggacat
     1621 taaagccgcc ttatctcctc actgtaatgt taatgtgtcg agattttgtg tgttgttgat
     1681 gttatccatt gtggtagtgg cgaggttaac ctctctccaa gcttcttctg ctgttaagag
     1741 atttatattt tacagccact cccaagtcct atcagaaggg aaagactgtg aaatcaatct
     1801 atggctttaa aatcagatat actctgtata aggcattctg tgttctacca ctttgacaga
     1861 ttcattccca ctt
//
LOCUS       KCVB01000031             815 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1278pri ultra-conserved element locus
            uce-599, sequence.
ACCESSION   KCVB01000031
VERSION     KCVB01000031.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 815)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1278pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>815
                     /note="ultra-conserved element locus uce-599"
BASE COUNT          227 a          219 c          109 g          260 t
ORIGIN      
        1 attttgctgc accacaaaaa cgtgactgtc ttctgtagcc tgcagcgcaa agacacacac
       61 tgtctcacac gcacatacac acacacacac actcccagac tctagcacac acactgatac
      121 agtcacagac acacacacac acacacacac agacacggtc gtcctaataa cgtccatatg
      181 tcttaaatta tgtgaaagtg ctgcgagctg ctggttccac agacatgtaa agactccttt
      241 attcttctct ctgccctcct tctctctctc tctctctctc tctttactcc cacctcttcc
      301 catctctctc tgccccttca tcctttctta aaatactttt tattcctctc tctctctctt
      361 ctcctcttag tcaaacaaac agtccgtacg tcttccctcg cttctatcat cacatcgaat
      421 cagagaagga ttgctttgtt gtcgagctta agctctttag gtgtaaaggt cagagcagcc
      481 atgtgaggtt aaagtcccac atgaggccaa atcacattgt ttcagctttt gatttatcct
      541 gaagcatctt aacagaatca caccagaaaa aaaaaaactt cttttctctc tatctgcatg
      601 tgctctttca gataacttga gtatttacat tttatgagac ttgtgaaatg tactcttgac
      661 ttcactgaat atccctgcag taacttgtta ctctgcaaat attatacatt gctacagttt
      721 aacctacaat actatacatt ttttgaattt tttgaaagtt tgagaaccac tggattaaac
      781 tactgagcag catttaaatt agtaataaat tgtct
//
LOCUS       KCVB01000032            1256 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1315pri ultra-conserved element locus
            uce-665, sequence.
ACCESSION   KCVB01000032
VERSION     KCVB01000032.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1315pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1256
                     /note="ultra-conserved element locus uce-665"
BASE COUNT          324 a          278 c          266 g          388 t
ORIGIN      
        1 gaaacgtcat ggtttagact gtctgcagat caatacctct atattctata tcattttccc
       61 tctgcagttg catgaactaa gctcatttca tcgccgctct acgccatcat gtcaatccca
      121 ctactccagc ccataattta gttccacccc attaggatgc tgttactgag agccatatat
      181 accggttata gtcatgaaga gttaatcatg cataaccaca tttcctctgt gctaaattgc
      241 taattaattt gattcatttg ctgttgtact ctctctgttt aaatgccatt gccaaagcct
      301 caatttatcg agcttgcaag cgttttactg tgcaatcatg tgcagagcat tgtgactcat
      361 tgcagctcca ctgctcatct acctatttgc aggcgttaaa gcacaatgct ctgtgctata
      421 aggctaacta gtgttagctg tcacactaac tgtgcacagt gcatccactc atctaattca
      481 gcatgctgac ttctggggct tttttttttt ttggtcttgt tttcttttta ctgacgtctt
      541 tctcttcttg tctctgcagg actcatctct cctggagtgt ctgtccctgt ccaagtccca
      601 gggagcgagc ctgacatgtc tcagtactgg tcaagactac agtagagaga agagctactt
      661 caccctgtaa ccacccactc caccatcgcc ctccggcagt gctcctcaca catacacggc
      721 acaggagagt agagaaggcg agagagggtt gagaagaggc aaaggacggg agaggagagg
      781 aggacagaaa gaaagagagt gactctggga gagccttggg acgtctgggc tttgtttttc
      841 cagtgggact gtgtgctgtt ctgtagctct aggcacattc aaacgaggac ttggaagaga
      901 gaacaacaat ggaaagaccg aaaaaaatgg acctctgtaa agtgcccggt gttaaaaatt
      961 tttatggaac aaaaacttat ctgttttttt ttttctattt ccaacttcag tcattgtttc
     1021 ctttttttct ctggtgtgtt agtaaatggc catttgtatg ttaatatgaa aaaccaagat
     1081 caagtactga tagatggact gctagaaaac catgttggtc ttgcgttttg tttgagagag
     1141 gagaacctga gagattctgc catgactatc ttttaaatct gcttatatcg agactttcta
     1201 ccagcctttg catggtctct ggacgctgtt gcatatcatt aaagacgaaa aagctg
//
LOCUS       KCVB01000033            1859 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1356pri ultra-conserved element locus
            uce-620, sequence.
ACCESSION   KCVB01000033
VERSION     KCVB01000033.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1356pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1859
                     /note="ultra-conserved element locus uce-620"
BASE COUNT          569 a          360 c          362 g          568 t
ORIGIN      
        1 aaatgcagcg atgacagcta tttctctcgt cttcctaagg caactgaagg ccatagcatt
       61 gtcaggctgc acggagggca atctgatgca acaaagtaga ttgacctaga ggggtaagag
      121 atgaagcagc gcacaaatcg tggcctctgg cagttcagta gctggcagtt acactgctaa
      181 tgtgctcgac agacttctgt tctgctgtta accacttgct gacctattgt ttatgtcttt
      241 aattaagtgt gaggaagtta taacaacttt gtacctttat ttgtccattg tcataataaa
      301 tcaatgctgc agtagaggat ggaaacactt attgaaaata taagccagat gaagatgtaa
      361 agtagagaaa gataatcaga tgtagccaca attctattag ttatgattta attaattcct
      421 ttaagccttt ttgtgttgtt aacattgaaa cacaacatgt gctgtagttg aatgtgtttt
      481 gtgtttttac caccttatct atcggatatt ttaatccaag cacaaaactt tggttgaagt
      541 cccaaaatgg attttgtggt tcttttatta gttttggata cgagctacag ttgctggctt
      601 tgcctttggg gtttgcaagg gttgcaccag cagaattgta ttctttagta acatagtgtg
      661 aaaagtttgg tctcccacaa aaaagttcct tgcgatcctt ctttacatgc acgatctttg
      721 atttctgacg atgtaagatc aaattgagct gcaagtgtgc tccctctctg cctttcatgc
      781 tgcttcaata tcacggcgtg aaaaaacaaa gaaggcaaag actgagggaa atctgaagcg
      841 ttctgccact caacatcccc cattcccaaa caacagcgtc tcactgccac tacaaataca
      901 ttagaaatca attatttatg gaacagagtc agggcctctt taatcttttc cttttatcca
      961 attaaattgt ctaaccattc taagcctcta tagctcacag ggaatgtgct tctttaaaaa
     1021 agaaaagaag aaaagatttt ctcttcagtc cttcttttga ctttcgatgt aacagagaag
     1081 agacacttct gagggttgaa gaatgaaagg aaagaaagtg agtaaagtgt aaatgaactc
     1141 cccctcccgc cccgtcacca ttgcattggt tctacaggaa ctatttgaag ttttgatatt
     1201 aagactctgg ttccattccc cgtgcaaagg gacgctgggg agcagcgagg cctttcatga
     1261 ttcctttatg cccagctgaa atagttcgaa agtatttgcg atcatctcca caggggtaaa
     1321 tacgatgttg cttcaattgc tgtcctgtat tattcaagaa gtcaattgaa gtagtgtctg
     1381 aaaattgaaa atgcttactt ccgatgcaga cgtgtgaaat tattgatgtg aagcattgta
     1441 aaacactctg cacttctctc tattatcata cagtaagact gttagagaag aaaagttaca
     1501 gattcgctca aaagcttcac atatttacac tttgtggaat cattttcacg cctcacaatg
     1561 aaagacatga tagcagaata atgcctccgc taaatgtgtc gtggtattta taatgaaaca
     1621 caaagactcc gagaccatgt ctacttctct catttcaagt tagataaccg agtcttcaaa
     1681 agttcaaaag tttaccaaaa aagggggatt aatggacaac aattataata atccattgat
     1741 catttaagtc attcaggaag caaaaaacac tcatcacctt ctttaaaggt ttcatatcca
     1801 tgtaaactga aagtgttttg gactctcagt cagcgttacg aagacgtcac tttggacat
//
LOCUS       KCVB01000034            1362 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1395pri ultra-conserved element locus
            uce-88, sequence.
ACCESSION   KCVB01000034
VERSION     KCVB01000034.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1395pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1362
                     /note="ultra-conserved element locus uce-88"
BASE COUNT          346 a          270 c          258 g          488 t
ORIGIN      
        1 attcaaatgc caaaccatta cactgacaga actctgaatc attttccctg tcatctcagg
       61 tgttctctca actgagttga caatttcaag aggatagttt ataaaaagaa atttatatta
      121 ttaacatcca tcttctgatg aaccatcaat ttggcggcat gaattgtatt gatacccaca
      181 tgcaatttcc tgcacgtaaa tgaagttgat gatctgccag tgcaaagcac tgactatctt
      241 agcatcctag tatagcagat tgccatgctc cagccctgcc tccattagtg ctgtgtacct
      301 gactttaata ctgaactgtg cactttagtc ttgtataaca atttaatggc cgctcctaca
      361 gcattgtcaa tgtgtctgca atactctgct ctcactagcc tgtgcagagc tacgctatag
      421 cctatcaagt agcgcagggg ccgaccgagc aagtgccgac catacggatg gacctatagc
      481 ttcattaact gatgattgca ttttctggta gggctcctca gtgtaatggc caactataga
      541 tgggtttgtt tgttcttgag tgaggaggac catgcagttc ctacagggac ttaacattag
      601 ttccattgag tgagtcgacc atctgcccac cattaacccc agaaccctcc ttctctttac
      661 aggctggaca atttgcttaa tatggcctat ggcgtaaaga ggtaattaaa aacttcaccc
      721 aatgccagat gtccatgttg actcaatgat ttttcaatat ccttttttct gttctttttt
      781 gagtttatgt ttccttgtat ttttgccgtc actttgcgtt tgaaagttcg ttaaaggtca
      841 gctgcatttt ttgattcttt gatttgatgg ttttttttcg taagcttgag ttgattttaa
      901 aatcctaata accagttaag gtgtattttt taaaaaaaac agaaaacagt aagtctttgt
      961 tttcagtggt gatgaatttg ctttggtttg attatgacaa gtgctggtgt tgtttggatt
     1021 gttttgggtt gtttctgtgt actgaatgga ttttgttctc tggtgttaaa ctccttattt
     1081 ctaggtgtgt tttgtttaac tggattcagg ttctcctgcc ttgtcactct acagctgtag
     1141 catgcgaaca ttccctctct tttggagcaa attctgaatt tgtcactctg attttatatg
     1201 tgtttcctca ctacatcata agaataaaac ctttgtagat ctactatcaa ctctgtacat
     1261 aaccctctat gcaataaatt tacgtctttt cagtgggagc cattacaaaa ttatatattg
     1321 ctgtaatttc ttggcagtac tagctaagat taggtagtgt tc
//
LOCUS       KCVB01000035            1536 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1466pri ultra-conserved element locus
            uce-335, sequence.
ACCESSION   KCVB01000035
VERSION     KCVB01000035.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1466pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1536
                     /note="ultra-conserved element locus uce-335"
BASE COUNT          416 a          329 c          332 g          459 t
ORIGIN      
        1 tgcaggcgac acaagatacc tgtaagtgac agttaagagt gaaatgacaa ctacaaggca
       61 ggcaggctgg tgatttgctg accccatatg atgcttaaag ttgatataaa gcagtttgaa
      121 ccttaagttg tgctttcaca gacctccacc tgaaagtggt ctgggaattg tcattttaag
      181 cgttgcatat ttgggcttca aataacctcc ctttttcccc aaatgcaggg ttttgagaat
      241 cgagataaaa tatggcatat caaatcttaa tatgcacgct tcacccacta gtgtgcaaat
      301 gatgcatcgc gggtccacat tcccagattt atgaaaccag aattttaatg actgtttttt
      361 gtgacatgta gtttaggaga ttgatttttt tttttttttt atgaaagctt atggtgactt
      421 tctagaattg tactatgaag ctctaacagc ttggatctta ctcaacatga atctgttaca
      481 tctgttaacc ccacattaca ttatgattac atacatttca aagaatttat ccatcatttt
      541 attccgtaaa cctttgcgtc ctggatgtga gttaatttgg actcctggat cgcaatctgc
      601 caaccttagg gcacccaaag tgcctactag ctcccctatc aaaacaatca tcagctgaag
      661 gaggttatcg ttaggctggg ctatctgtca cccggccacc gccagccagc gggacacaag
      721 ggccatttaa ttaggcctac tgctcctctc tggtgtgaaa gactctcagg cccctttgat
      781 tccccgctgc tctcccagca ggaagtggtt ggtgataatt gacacaggat ttccttgtta
      841 accagcggca gactctgtgg attaaagtga cagagatctc cagaagaaag tggggagtag
      901 ataaggggga ggggaggagg tgcagtgggg ggagtggtgg gggcagcagc tatttaccgc
      961 tcttgagaaa ccagggagga aatagcaata tcagactggt gcgcggtcct gccgcgagat
     1021 gtagctccca cttatcttag ccatttcagc ttggctgatc aaagattggc tttccttaaa
     1081 ataacctggg cccaaggttg agtacatgct tccacgccac gctttttttt tttttttttt
     1141 tttttggcaa attatctgtc ttttccaatc ccccctataa agctgcatgg ctgtttgtgg
     1201 aggactagag agataatctc atttcattta tacaattata cactatcatg gaataaaagc
     1261 atatttgcaa atgacgtaat ttcccgcttt gtaccaactg ggtcacaagc cttgacaaaa
     1321 gcattatcat gcagacagga gaggtaccca gtcggctgta gggcagaaat ggagctttca
     1381 gaggatgtaa tccactttat ttatttctag atttcctttc tgctcctcca actactacac
     1441 agcttaccaa aagacatttt acaagtgctc attggaaggt tttaacatag ctcttctaga
     1501 gataaagatg aagcagcgac tgtactctcc actttc
//
LOCUS       KCVB01000036            1638 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1509pri ultra-conserved element locus
            uce-370, sequence.
ACCESSION   KCVB01000036
VERSION     KCVB01000036.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1509pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1638
                     /note="ultra-conserved element locus uce-370"
BASE COUNT          499 a          341 c          369 g          429 t
ORIGIN      
        1 aatacaaact tccagtctca ctgtcaagct ctgctgcgtg tatgttattg cagtgacaag
       61 aggtctccat atacttgtcc tgattctgag acaaatgggt aatgaggaaa atcctggcgt
      121 cacttgcgca gagaaagaga actccacaca gcacacatca gaaacagaga ggcatgaaat
      181 aggaaggaaa tgtctgaaac ccccagactt gacagaggac tgaggaacag gggataattt
      241 gttcttcttc tccatgctga attagatttc catttttggc taggttaaag ttgtccatcc
      301 aatcgttccc ttcaaccttg acgtaatctc cccatctgaa tgccataatt cgctccagag
      361 gtaatgcaaa atgaacagat ctgtatgcaa atgaggcgca aacgtcaggt cggcttatgg
      421 aacggcgtgg atgaggtgtt gtgcaggaca ccttttgatc tgttatttca cttacggatc
      481 gggttcacct cccaagcaac ctcctcacct gactgcgaga atatgtgtgt gccagagtat
      541 gcagccgtgt gtgttgaaga gacctggtac tttttgcctt gcatggcaga acataaatct
      601 cccatagtga gttcctccct ccctccctcg ctctctcttt ctctctggaa aggcagtcag
      661 ttcgttgggg acacttccct tgtgggcgtt aggatctctg tggggggtca caggtcagtc
      721 ggcggaggaa gcgacaagct gtgaaatgta aaagaccaat ttcttccaca tgtttcagtg
      781 ctgggagaac actgccaagc tgtcagccgg catcacgttt aaaatgggcc agggtgctac
      841 ccagcagacc tgtcacaact catttgtaat taaaaagcct gtgtgatgtt taaagcagag
      901 caccccagaa acttttgagg actgttaaat aaagatgagc tgtgcgtgca aaaaaaaaaa
      961 gagaaggaaa aaaaagggga tgtattaata acaaagaagc tagccagtcg cctttgtagt
     1021 agatcaatac caaatgagcc tgtacttgat tcctggcaat agccaggatg ttggattact
     1081 tgaaactagg tgggaaaaag aaaaatctgt tttttaaaat aagaaagtgg atccttgttg
     1141 ctattaacag aagtgatcag ctgcttacaa tcctgatatg accatgcctg gaccaccaga
     1201 tgagcagggc aaccacatca gaatagttca tcagtgcatc tgattaattc aggatcatat
     1261 tggggtttat tccagcatgt acttaatgaa aagcaaaaag catctgtcca taacaggact
     1321 aaaacatgga aacataatca cattcacacc tgggaatgta agtctcaact caacatgacc
     1381 tgcatgtctt ctgactgtag gaggaaacca gatcccccgt aggaaaatca tacaaatgtg
     1441 gaggaagact gtatgttcca cgaagagaga cccttcttta tgtgagcaac aacatcactg
     1501 accaactata aaggcgctac aatagttcat ttgctgtctt aagtcattta tttttgtatc
     1561 ccagtgattt ggaaagaagt gatgaaaaat gaaggatata gattacattt gaaacttctt
     1621 actggtggat aataatct
//
LOCUS       KCVB01000037            1510 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1588pri ultra-conserved element locus
            uce-719, sequence.
ACCESSION   KCVB01000037
VERSION     KCVB01000037.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1588pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1510
                     /note="ultra-conserved element locus uce-719"
BASE COUNT          459 a          303 c          294 g          454 t
ORIGIN      
        1 gaaacaaaaa aaaaggacaa aatatgatat tgtatgtcag tttagccttt gtgtgaagtt
       61 cattacatta taaataaccc tgtacagttt ggagacttac gtgcacgaga tcctgtgctt
      121 cttcttaacg tgagcctcct ccccaggcaa actgacatga caagtttata aggaaacatg
      181 attgagacat cagcatttaa agtgctgctt ttctcacctg acctaaaaca gaggtttccc
      241 ttttgtgtca ccttaagtaa catgttataa tttgaattaa ctgcggggaa ttcactcaga
      301 ggctctaaag ttacattttg atgcccaaca cagagggggg aaaaaaaatc taaattagga
      361 gttaaggtct gataatacaa ctgaacaaaa tgagcgaagg agaaataagt atttaaataa
      421 ttcagagagg gcataattaa gtgtttaatg cagtgttgta gttcaaagca acaacaaaga
      481 aattttaatt caaattagaa atgtaaaaga gacgcagatt cttcagagat gagaactttg
      541 tgttgagtca cttgatttgg tctctttcct ccatcaaaca ggcaggcaac gctcttggag
      601 atctccagag ataacaatgt cacattgtca catagggttc tgctacaaat ccttaattac
      661 aaaaagagga tcagatgact acagcatcca ataccggggt ctccaggcgg catgggggtc
      721 tttgatcaag aaaatccaat tatttgtgta tatacatttc ccacatagtg attacttcat
      781 taattgtccc gcctttatct cctcccttcc catcccccct aataatcagc tcctttatcc
      841 agaccaacaa acacaccata ggagctttgt ggattcaaag gatttgcttt cccctctcaa
      901 agagccgcta ttctttgatg attgggcagc ggcaaacttc aaagtaataa atcttatttc
      961 tgactggctg gcggcccacc aaagtcctta tcaaattcta agaagtgatc cctcactctt
     1021 cagatagacc aaggatatct cggagagagg agccactgaa gagtggagac ctgctcgaca
     1081 cgatattttt tactgcgaat gcacttattt atctaaatta gcgcttttat ttttatttgt
     1141 tttaatcgag agggaggagg acgtttacca tggcagcagt ggctgtactg cggaatgaaa
     1201 cactccaggc ttttcttcag gtttgtgcgc aagtttctgt ttactttgac gactttaata
     1261 acttgtaaac ttactcaccc gagttgattt acgcgttggg ttacgagtct tttatatttt
     1321 acaaagatag acgtggtgct accttgatag tccagtgtca tataatttaa ttactgccgc
     1381 taattgtttt gcgtgttaat gacttctgct gcaacataac gccaactttt atgtcacacc
     1441 gcgactcggg atcgatttaa agcagatgaa attcaagcta ttcacacaag ccatgacatc
     1501 attttgttgg
//
LOCUS       KCVB01000038            1284 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1615pri ultra-conserved element locus
            uce-877, sequence.
ACCESSION   KCVB01000038
VERSION     KCVB01000038.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1615pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1284
                     /note="ultra-conserved element locus uce-877"
BASE COUNT          360 a          231 c          259 g          434 t
ORIGIN      
        1 acattgcata ttaaatgttt ttgaatggta tgtacttgta ctctattcat agtattttta
       61 aacttgaaat ctgttgtcca ataaatcctt tgaagattat tgtgtgtgtg atgttgaatt
      121 attaactcaa taacagcttc ctttggcaca cttccttcta tttaaacaca tccctgaact
      181 ttttatcact cctcttccca tcttacaagc ttcacaaatg actgttgatt caatgactgc
      241 caagatcaga cgttgctttt agtaagaaac caacaatgct gtgactcctt tgcatcaaag
      301 ctgtgcagta atcacagttg catcaaggca tgcttttaat aaggcttttt ttaaggacgg
      361 catcaaaata attcactggc gtcaatatat tgcagccact tataaactac atatccctca
      421 ttttgagatg catgaaattg tgatcatgaa cttggaacac aatctttcta gctgagcaga
      481 gttggggatg atattggtac aaaaatgcca tgcatgatgt gggtgacagt cgagactgaa
      541 tgtgtgatat tagagttaat taaagctttg cgccccatct actgcaaatg cttgcacttc
      601 tgcaattaag aatcaaaggc gagggcttag tgtcaggggg gtgtctaata catggtttta
      661 acaggaatgg gacctgattt gccaagctcc tagcgacaag cattaacaac agcctcaaat
      721 tagagggaca ttaatattca taagtaaaac aattcatcct gtgaatgtgt gatgaaatgg
      781 aatcctctga ggtgagctct tgtgtccagg ctgccatcgt tcattaaaat atgccattac
      841 ccatgaagtg cttaatcttt atgaaaggat ggtaaaatct gtttgaactg ctctgacaga
      901 aaggtggaaa ttgtaatatt gttcagcacc ttgcattatt ttaatcccgt gtattttttg
      961 aacgtgattg tgtcgaacag tatatgtttc tatatgtctc attagctgaa ttgttaaggc
     1021 tctctgaatg aaaatgtatg atatataatt tttgcttgcg cccattgata ttagtctgtt
     1081 actctattaa gacgacgtga gttctttgtg ttgttttagg atagctgttg tacggcgcca
     1141 catgcaacac aatctaaatt gtgtgtgtgt tcgtgtgtgt gtgtgtgtgt gtgcatgtgt
     1201 gtgtcttaac gccatcccat tgcgatggaa ttgaaatgcc ataacagcga cagtttgtcc
     1261 ggctgcttct ctccacttaa tagt
//
LOCUS       KCVB01000039            1727 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1654pri ultra-conserved element locus
            uce-30, sequence.
ACCESSION   KCVB01000039
VERSION     KCVB01000039.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1654pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1727
                     /note="ultra-conserved element locus uce-30"
BASE COUNT          500 a          369 c          300 g          558 t
ORIGIN      
        1 tttttttttt tttttttttc atccttccgg ccaaatggaa attcatcttc cagtatgtca
       61 tatctattaa gccattttaa ttgccccgct gtacaagttt ggggtattta tctttacttg
      121 tttctatggc actgtcacaa ttcacacaaa gccagatgaa atcagtacat gcaacatgaa
      181 gtgcaaacat actgtggagg agcagatccc agtacagcaa tcaggagaag gatgatttat
      241 agccacaaag gtgtcacttc gatgtttatg agctctagtt tttcaagtca aactaagagc
      301 gctgcagtgg tgcatttctt accccgctat tattcactgt gtctggcaca aaatgtccaa
      361 agcagttcaa caaagccgag atggacaaca gaaaaaagtt taccattcta tttttaactc
      421 ccaaatagca attttctggt tttgtggcgg cagtgatctg tcatgtggct gggaggttgt
      481 gctgtttgcg ctgatatcaa ttaaaagtgg attaacgggt acataatgac ctttagcagt
      541 aaattgtgtg taatgttgca aggcaggata cagtatattc cccagatgtc acaccaggcc
      601 cctgacgtat gatggagcag atgtcttagc cagtccaaat ggccctctaa gacacaaaca
      661 cacacataca tacacacagg ctcaggccta caagcatgca taaacatgtg ttcacacaca
      721 ttccaaatgg tgctcacaca cgtacgtaaa cacaagtaga tgcagaaatg tacttgtcgt
      781 ctgtctcact cgctgaagat ttatttaaat gcttaatttt aatgcaaaac tggaaaaaaa
      841 actgataact ttctgagagc atttgtattt attttcagtt agccttactc cgtaaacatc
      901 taataagaat gcaaaacaaa caatatcctc agcttttcat tctcatttta gatatgtaga
      961 gggtacatgt tagatggtac tcttaacccc cccccccccc cccatgaaca ttaacaacct
     1021 cttataagag aagaaaagac agaggaggaa aaaaaagatc agatcagtct aatttctgtc
     1081 taatttggct cctagaatat cattttgtcc agttagagga attgagaatg atcatttgca
     1141 tcagggacgt ttccttatta aatcagcact gagcctctgt tcggcagctc acaggagaag
     1201 cacttattgg gttaaaagct catctcatat ggtttatttt aaatatactc tatgcctcat
     1261 agccactcta gcgtaagaga aagatatcaa atcactgtct ttctatctat agtcagggct
     1321 atctgtctct ctgcacgctc cctttccctt ttttgtctct cttattcttt ctctctcctc
     1381 tcttcaacat ttccaccttc aattttcttt tgtataccct cctatccgtt tctctcctgt
     1441 atcaatattg tattgagttg gtgtttgtgg ccctgtattt gagaactctg cagtaggtct
     1501 gcagatcgca gagtcttttt ctggaatggt gccctctaaa taatgataaa ttacttgagc
     1561 agtatgccat ttgctaatta tatccatgat ttactacaca gcccgactta aatcattcaa
     1621 accagccttg cgtaacaatg cctttttttt cttttataaa atggtttcca tgtgtataat
     1681 ctaaacattt tatggccata cattgtatat atagaaatgt gcataca
//
LOCUS       KCVB01000040            1421 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1724pri ultra-conserved element locus
            uce-290, sequence.
ACCESSION   KCVB01000040
VERSION     KCVB01000040.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1724pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1421
                     /note="ultra-conserved element locus uce-290"
BASE COUNT          467 a          248 c          245 g          461 t
ORIGIN      
        1 tactttaagt tttgacttct acaattaata cttgttaatg aaagaagtaa tcatttcact
       61 tttagctaaa ttttaaaaac cttacatcca attattgaat cctaacttga aattaaagct
      121 gtaatttaat acctcacata tacaacagcc atttctgctt tactgaatgt atagttcaaa
      181 gaattacagc aaaacccatt ttgcttaatt gctttacttt atttaaacct tgctgattag
      241 atttagattg agatacattc ggtgtaaatg aagataaaaa cattttttag gtgttaaaat
      301 atgttttttg tacaatttgc tgcagctcaa tatcagagag atgtccctaa tgttatgttt
      361 attatgttta tcagaagatg tgtagttaag atgtttataa cacattttta ctttaatctg
      421 aagtaaaaaa aaatctgaaa tctgaagcct gaaatctgtc agttttggca aaaatgttaa
      481 tgattaacta ttgtatctgg cttttcttgc caaaacacat aatgtctgtt tttcatggta
      541 tttataatgc tggaattgga tcatctgtgg aagagaagag cagggagagg agagcgaaca
      601 cgggggtccg ctgagttgtc tcagtgcctg aactttcctg tttctccagt agatgccagc
      661 agtgaaccat ttaaaacagc cacgtcgctc cccctgtaat tcgagggtac tctgcatgta
      721 actgctctgg agttggagat ggtacagatg gctggcctaa acgcaacact tatccagaca
      781 tttcactgtt cgcagaatga tatcaaagtc aactcaaaat ggaggctgtg ccatcaactt
      841 gaaacaaatt ctaacacttc caacaaaatt gtattgtgag taaagtcgcc gtggatttgg
      901 tcagcgttca gcacagatgt cccccctgtg tgtgtttgcg ttaaataaaa actagtgctc
      961 acatcctctt aaaaattcag ctgtggattt tcagggataa atgctcacaa ttataatgtt
     1021 ttaatccaga ttggattcag acacatttat ttatttcaga gcgtgagagt ttaaatcgtg
     1081 cataaaaaaa aaaaaaaagc ggtgccagaa caaaaacaaa tgccctaaat tatgtgaaat
     1141 atgggagaat aactgaaaca gcaacagctg gttgctttac tcttctaggt gctgaagaaa
     1201 tgtcaaactg attattaaca tttagataat ttcgtacata tatttaatat aattccagtt
     1261 gaaaattaaa tacttttcct cctcttctta aagaaagtca tcatatttcc cacatgggct
     1321 cacagacctg ctgtcatttt aacattacag tgcacacgac acatcacatt aatatgtgtg
     1381 aataaaagaa ggcaaattta tccctcaatt tgtcttccag g
//
LOCUS       KCVB01000041            1477 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1753pri ultra-conserved element locus
            uce-604, sequence.
ACCESSION   KCVB01000041
VERSION     KCVB01000041.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1753pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1477
                     /note="ultra-conserved element locus uce-604"
BASE COUNT          380 a          343 c          360 g          394 t
ORIGIN      
        1 taaactgcga atttaaaaat gtggtcaaca tagtacctac agatatttgt gtataacaag
       61 tttgtcaaaa gatcacgact ttgcgtcttt tatgttgtca gataaatatt gcagtttaaa
      121 ttaattagcg ttatctgcaa taaaggctta atgaatgaat aacatcaaat agaggtttgt
      181 ttttcagtct ctcaggacca gcccatatac tatagtgaag ctgacattat aacagcacta
      241 tactatatac tataggacct ccccttgaac ctctgtaact tgaagctcaa ctaccaccat
      301 cattgtggtg gtagaaaaaa aaaagtccaa gtcaaaaact gcagttctga cattcctcgg
      361 ctccgttccg atgcacagac cgcgtacctc gcctcagact tcagctccac gccggcaaat
      421 gcttttttct agcgacaggg cgctgtgtgg aaacccaaaa caccaaacgc accgagcggg
      481 tttggctctc agggtgcctc ccctgaagtt ggagatgcac acatgaggtc tgtctgcctg
      541 ggtggtctct ctactttagt gagctgttgc taagcagcta ataatagtcg tgtttgctga
      601 gtaatttttg cccttctgga accaatcaga tgcaagaaag tcctgcaatc tgacagcccc
      661 agaggcggga tctccagtct ttttttttat tttcacccct cctccttctc tgcctccaaa
      721 tggagagagg tctttttctc ctcttctgtt gaatctcaga gttggccgtg acgcgcggcc
      781 caggggaaga ccacatagat ctcaccagaa tgctacgacc atcgaggttt cttacccgac
      841 gacttgtgta tgggaatcac ttcgcgcagc tcgctttttg agagaaactt acttttttta
      901 agagacaagg tgaatgcggt gaaagcggtg gagacctccg gatcacgaaa tgttgggatg
      961 gaaggcgcag actttgactc gccgtctcca acaggagtat gaagtcctac ttcacgggtg
     1021 tgaggtctcc gagagtgaaa ctttgttgcc ttgggatatc gctacacgct ttcggggaaa
     1081 tactataaag tgggaaacgg taaccatctc gcagtccccg caacatggcc tcggctgcta
     1141 atgcgccctc cgagcaggaa aacagagacc ccgatcggcc gaggataacg cggaggaaca
     1201 ggggggacta tacccgcaac acaccgctgg atttggagta tttgcagcgg ccgagttact
     1261 gcgatgcggg cttcgctttg gagcaaatat ccgaggtgct tatttctcat cttaacctgg
     1321 aaagagctta gtggtcttgg tttaattgta ttcgccgaaa aaagtgtcca aactatgtcc
     1381 agttgctgct aggtaacggg acgggctggg cagggggaca cagtggtaag gcaaggttgt
     1441 agtgagccgc gagttgtttt gttttatttt gcacaac
//
LOCUS       KCVB01000042            1846 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1834pri ultra-conserved element locus
            uce-540, sequence.
ACCESSION   KCVB01000042
VERSION     KCVB01000042.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1834pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1846
                     /note="ultra-conserved element locus uce-540"
BASE COUNT          510 a          447 c          437 g          452 t
ORIGIN      
        1 tctgtcattt tcaagctgaa ggctaaactt ctcagtgctc tgtctgagtc tgaggcattc
       61 agtaataatg acaaaaaagc gccaaactgt ccacttctga aaacatactg atgcagatca
      121 actctctgtt ctcacaggca tgagtcaatc catgagcgct gtgtacaatc acatcttctc
      181 ctaaatggca ccggctgtgt gaaaaatacg tgacgagata atttgataaa gccgtgcctc
      241 tgttcaatcc aacagataat agccggggaa ttacgcaaat taggccgttt ggtttgagca
      301 aaggtgtggg gtgagcaagg ggtttttgaa ttctgccggg aacgtaaagc gtctgtaaat
      361 acgaagcaca aagagtctta acgcactaaa cctcgggcca cactggccat acaatggact
      421 gtcatattga attggcccag agctgtggga gagaggctgt ttaagaagac gcagggtggg
      481 agttaagctg agaaagagcc cagcgtgaca gacacttgtc catgtaacag ctctgtagaa
      541 actcacaccg ggctctcaac aacactctgc tttactgtcg atcaccaaga cactcagcaa
      601 caccggctgc cacccatacc tgtaatataa atgcaatctc actttttctc tgtgatatat
      661 atcgaccatc tcagaggggc agacagagac tgacacacat gcacacacac tcacttttga
      721 atacagcacc atacagacag tcaataacag aatggggtgt gagattaagg ctaagcccac
      781 cttcttttca tgtggcagag cagccgctgc cccgagatct ggcaccagat ggcgctgtgc
      841 cggtcggtgc caggagataa tcgggacaga tcgggccgca ttactaccca ttatcttcta
      901 attattcttg tgtggagcgc tgatcctgcc gtttgtgtaa gtggatggca cagagagcct
      961 gtcgcccccg gcagcacgaa aatccttccc acttccctca ccccctctgg ggatagaggg
     1021 agagatcagc cgggggaggg aaggagatag gggagggaag gaggaactgg tgggtaaggc
     1081 agccaacctg atagagggga gatacgtctt tgtgagatat aacacggcat ccaattttca
     1141 tagtgacacc aatgatgtca gacgggggag atcctgacct ttgacctctg tgttagtgtc
     1201 acataaagca acatcctcgt tctttcacaa aacctgagcg agaacaccat ccttctcctc
     1261 tgttgactct ataatagtgt gataaggata cagttcaagt ctcccaggct ccatttgtac
     1321 agtgcctgtg tggaaacagg gcccaacccc cctaccccaa aggataggat aactgcgcag
     1381 aggcatatgg cttaccatgt gctgggtccc acgtgcacac tcttaagcgc gattctaccc
     1441 agaacatctt cgcttaatta tcagcatttt gttcagcatc ctcctatttt gcatattaat
     1501 atctgtcagc tcagtgtgta atatgaggag atgaaggctt aggctacgct aaaataccac
     1561 tgtaaacgca ggcttcacca agcaagaata tacatattca gctgcatcga ctcccacagg
     1621 gtgcagattc catgatgatc ttagtgtttt caaaaccttt taaccaggtc tcatgtgtgt
     1681 atgtatgcat atatgtttgt aaagtgtcaa aatctggagg caacgcacaa gctctgtgtg
     1741 tgttttgttt gcgagcatgt acacgtcgtg cgcgagtcac aaccaacaca acaccaacac
     1801 gtgtgcccat ggcatgtggt aaccagcctt ccgactgaca tgtaat
//
LOCUS       KCVB01000043             840 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1855pri ultra-conserved element locus
            uce-191, sequence.
ACCESSION   KCVB01000043
VERSION     KCVB01000043.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1855pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>840
                     /note="ultra-conserved element locus uce-191"
BASE COUNT          292 a          143 c          175 g          230 t
ORIGIN      
        1 aaaaaaaaaa aaaaaaaaaa aaaacaagcc gtctgctgaa ctgtatctgt gtctccagca
       61 gggcagctga tttggtttgt gaagtatgtt aataatcaaa tgatcaacaa ataacagatg
      121 tattagttca tgcagcttga aatgtcaagg aggctaaaag cggaaatcta atacagaaag
      181 cccagttgca ttgtattatt ttccgtgaca aaccctccgg tccaaattat gtagggcctc
      241 ctacagtgag taatctttac agaaaacggt gccaatattt tgtaattaag ctgcagtaag
      301 acaacgaggg ctgccacaga tacagatgga agttgcagaa cttgtaacac tttttcttct
      361 cagtgtggag gtggatgggg gaagttaaaa aaaaacaaaa aacccatgat tgaaaaggaa
      421 agtaatgaat gaatacctga cttattgaat tactcactct gtctccagct aacaagaaga
      481 gtccttgagt gagaggaagg aataccagct ctcgtgccgt ttccacctcc gtcacatggc
      541 cctcatagga acacaggaaa ggtagagagg cgtcgcacat tgctgcgtga taccagtcct
      601 aacctgcaaa ttttgtttag ttacattaat tatgttcatg ttaaaagtgt taaatgatga
      661 gacaataaca tgcagtatgc tgtaaaatat aaatactgat ttatttgaaa tgaatttgta
      721 tgaatgaata aggttgtgta aagaaataca gtataaagag gtgttaaact tgcttcttag
      781 ccactgtagt ctcaataatt ggtctgctct gtaacccgct aatttcagcc tgaagtgtaa
//
LOCUS       KCVB01000044            1734 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1892pri ultra-conserved element locus
            uce-505, sequence.
ACCESSION   KCVB01000044
VERSION     KCVB01000044.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1892pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1734
                     /note="ultra-conserved element locus uce-505"
BASE COUNT          515 a          331 c          331 g          557 t
ORIGIN      
        1 tttcccatgt ctgcggtggc gttctataat tctattaaaa aaaaagcatt ttccttgcat
       61 tggcatactc tgtatttcat aggctccaga atcacggaca cgaaacaaat tgggttgaaa
      121 caaagctcaa gatctggcta atgagggtta caatcccttt gggttaattt gtaatgttga
      181 attttgcacc ctaactaaat atcatgtctt agcaaagata ctttggatga ctcagaatct
      241 gacataattc tttcagcagt gatgcgtcca actagaaaac acaggatgga aaacatgtaa
      301 aaggtgtcat cttatttttt cgttgctggc gacgctgcaa aagttacatc tcaagtgaaa
      361 gaggagcaag catattccag tatgtataca ggactgaggc taattccagc ttctgttcag
      421 taattgtttt gtcacatgct tttagtaaat tgtggggcca ctccagcatc ttactctaca
      481 ctgacttttt gaactgaccc caaacagcga atcccttgaa acttctcgaa tcggtctttt
      541 tatccgtgac agctttccag ttcacttgca gattgcggca gcctttcaga cggcgcacct
      601 gttgcttgag ccttgatttc gctttttgat cagagagccc tttgacaaaa gagggaaaga
      661 agaggctgaa gaagagggag aggcaagaga aaagatcctt atcgaatgct ctccaccgga
      721 caatgaattt tccccaacca ttcaaatgta tctgcagaat gcttgagtga ttcattacag
      781 cagattctga agtgtgacaa tggacctcag aaatgagtga tggtatcttt ttgctgattt
      841 catttccctg atcctcctta tccccctggc ctgtgtacct ctattaatta gaaagtaggg
      901 atttccactc atcaactgat gggtttcatg ctctgctctg tcacaaatct cctgtccacg
      961 ctatctctct gcttccaacc catggctgct ttggaacgaa catactctgc tccggcttca
     1021 atctcagctg caagtcaggg tggctttgtc gattcataaa tatttataaa ccgattaaga
     1081 aagaataata aagttataag acagtggttg gttacccttg aacagtgccg cagatgactt
     1141 cttaaggagg ggaaatgtga tcgtcctttc tgctgctttt ttgatgctgt ttttattagt
     1201 gaatcattct tccttatcat tttgtacata aaaactgtta gtttattatt ttattactca
     1261 cctgagcaac ttaataaaac aatcatatgt aatgtctaat tattagctgc catatagtca
     1321 aaagattcat aaatatgcag ccaaagtgca gaagcattat acagtagcag cagaatgatg
     1381 caaagttgaa atacaaagac aaaccaatga cattttaacc cttttgttaa gctcattgag
     1441 ctcattattt caggtccttc gtgataagta tgaatgcaaa attaagtttt ttctccttta
     1501 aatattttga tattctgatt aaatccaaaa aaaggagaag taaagtggtg atcttgcagc
     1561 gttaaaggta aaaaaattaa aaatgtatac gttatatggc catgcatcaa cgatatattc
     1621 tttttttgtt gtttgtgtgt ttgcacaagg tcacagacaa aaaaaatgta aagtatgtca
     1681 cagtacgtac attatgttct tatagtgttg ttacttgttt gggttcagtt agac
//
LOCUS       KCVB01000045            1626 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1936pri ultra-conserved element locus
            uce-997, sequence.
ACCESSION   KCVB01000045
VERSION     KCVB01000045.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1936pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1626
                     /note="ultra-conserved element locus uce-997"
BASE COUNT          487 a          342 c          351 g          446 t
ORIGIN      
        1 tgcatttgga gtaatttacc tgaagtaagc ctgagggacc tgcaaaggtg aactcctatt
       61 taccatcatt aaaactacaa atcacttaat accaatataa acagctcagc tattatgttg
      121 tgcagtgctt gggagttcac gcatggataa gcaaagatct gaatcacgga ggatgaattg
      181 aatgaattaa aacagccctt ggtaacaaca tgattaaaaa gctgttacat cagaatcaaa
      241 ctgcacggct gacggtaagt ccaatactta aaagaatcac accgaaaaaa gattgaatgc
      301 tgaatgcttt aaaatgatta aaagcagagc agtaatttat tttgatgaca gaaatgataa
      361 cctgcagtga tttgtgcagc agttaatctt catctaaaca ggacttcagc ctttctggcc
      421 atctaaccct acagaggaaa ataagaaaaa aggaactaag ccagtgtcat tgagaacggt
      481 atggaggtag cagccagccc cttaccctta actctgttgt ggacagattt cccctgacaa
      541 aaattaaact agctcctact tggcacttta ttatctcttc aactgaatga tttattgttt
      601 gtggagcgaa agaaccaaag agagggggaa gattacattt ccagccaaag ctcaggcctg
      661 atctcgtttt ttactccctc tgtccaggaa aaaagcttcc gacaaacaat aatgtcagct
      721 gtaattgatc ggaggtcgct ggctgggcct gcagtaaaag cgcactattt aagcgacaat
      781 cggccccgcg ctcttcacac gctggctaat gaagctgtct gtctaggcta tttaccttct
      841 cttaaatgga aggggagaga tgtactgtaa gagacagggc tggagaggat ctgcctctcg
      901 ctcctgtgaa ataggacctg cacatgcctc aggaatgtat aattggttgg cgcagccaaa
      961 gctggggatc cggtgctaat taaatgactt gtttaaagcc tatctgtctt tgtttgggag
     1021 gctctgggct tcagaacaat gcattgtaaa tgctgttgtc aaacaaacat tcctggttat
     1081 cctttgattt gaattgaaca agacaaaggg ctgatttgct cagcgctaag cctttggttt
     1141 tcatggcgtt gagatatttt ttgctcttct gatctgtcaa accaaacagg accattccag
     1201 tcaagcagct aaatgaactg actcactgca caatgcatat gcagtctgct gctgcaaaac
     1261 aatctcggtt ttcctcctaa ttgcgcttgt aaaaagcccc catgtcgtgt atgtatttct
     1321 ccaacaaagg tgagacggcg gtgcacacac atggagagtc tttgttgtgg tcgtatcaag
     1381 gttttgcaca ccagtcccaa aaggaaatga atggcttatg acaaagtatc tgtcatgcgg
     1441 caagacattt agaggtctct ccatagcact aatcagctat ttatctttag cataactgtt
     1501 aatctcttcc aaaaatcaga agtcatgtct gccatgtgag atggtgggga gccttacagc
     1561 cattagatca aacatacgat gcagcctcca taaatatgtt gcctggcatc gtggtgccac
     1621 tttaca
//
LOCUS       KCVB01000046            1668 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 1979pri ultra-conserved element locus
            uce-215, sequence.
ACCESSION   KCVB01000046
VERSION     KCVB01000046.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="1979pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1668
                     /note="ultra-conserved element locus uce-215"
BASE COUNT          489 a          321 c          317 g          541 t
ORIGIN      
        1 gttgactagt tatactacat tcttttgtcc caaatagagg atcacctggt agaccttcaa
       61 taatggcaga atgaaccttt cagaagcttc attagaatat ttacatttta agtttagcca
      121 agaactttta tccaaagtga ctttaagtga gtgagcaggc agtagggttc agtcaggtca
      181 catgactgct gatgaacaca acccttgacc tatgggctat gggcaggtca ttcttactgc
      241 taggccagcc tgtcaacaat gttagtgtac ttatatatat atataaaaag gtatcctgtc
      301 acaatggaag gaaacacaaa ctcagtcggt cactaccctc caccccccac acacgatctc
      361 cttaattctg caacgcgtag acctgcacac agatgcacac gttcactcga tggtgcagat
      421 atacagatcg agtaaagcca ttgcacacag tttgctactt ctggttcaga aatggtgtta
      481 gagtaaagta ctgacagtca tgtgatcaca attgcaggcc ccattcttac atcttaataa
      541 atgtgccttt tgatttgagt aatcaataac ttatgatttc taagtaactc tgtgttaagt
      601 actatttact ttttgggtta ttgatttctg cctatgtgca tattttcaaa tgtacgtatt
      661 tattagtttc aaagtaatta atgagatatt acctgtttat ttatttattg agatatttat
      721 tctgattctt tatttttttg agacaggttt ttatcttccg tgctgtctta cttctgactt
      781 tgtgccaatg tgctgctttg aggtgttaca tatcgtgggt ttgtatcatt attaaaccct
      841 cactctatat tttaaatccg tgtgtacctc tctctcacgt gtgcatgtca ttaactcact
      901 gtgccttttc tcaagcactg tatgcaagtt gtaatctaaa attattccac aatgctatct
      961 aaggactatg aaaaaacatc tgtaaatgtg ttcttttgtt tccagccttt gaatattttt
     1021 atattttgta aaaaaaaaaa tttgggaaag tagttttaca aacgtggaaa tctcgtgttg
     1081 gaacagaact acgcagaaat gtccagggtc cgttcgaggg aagtaataga acttcagtaa
     1141 cgttcctcaa gtactgaagg gaagaagaac agtgacacaa atgttacata aaaaaacaaa
     1201 acaaaaaaat taactttatt ctataaactc aagtctttcc ttgacaggaa cataggtgta
     1261 gtttgctgtt gtccttcaga agctgtggtc agttacttgg cagaatacat gtgctgcctt
     1321 ttatgtttga ggatctcagt ggaggtgagg gtcaggtctt cattgcagac gtcacagtga
     1381 taaactctgt taagactgga cactgaggag gagcctgact gtccaccttc tggctctgaa
     1441 aaccacaaca catatagttg gtcccaaatt attcatctct ttcaaactta tagccaatat
     1501 caagtatcaa gtcagctgct gagtgagtta aagttagata atatcaaaag atacctcaaa
     1561 ataaatgtca acataagaat tttatgacct tcatctggct gctgctgctg ttctccagct
     1621 ggcctgcagg aggcctcgtg ctgcatgcgt tccaatggag tcagaggc
//
LOCUS       KCVB01000047            1491 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2027pri ultra-conserved element locus
            uce-1126, sequence.
ACCESSION   KCVB01000047
VERSION     KCVB01000047.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2027pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1491
                     /note="ultra-conserved element locus uce-1126"
BASE COUNT          429 a          306 c          283 g          473 t
ORIGIN      
        1 tgctacacaa aaaatactgc tgtgtaaatg tcatccattc aacaaatgta aaataataga
       61 aaaatattaa ttgtgtttat atttgttttg ccaagttatt ggggactttt tagaccattg
      121 aggttacatt tagattatga aaagtgaata tggtgtgtac tagtgcagca aatttatccc
      181 caagttgatc attcccaccc ttaaaaacta ctactgtgaa cttcccccac tacccccttt
      241 attccactca aagcttaata ctgcacagtc cagaagctcc tttttcctct tagtcttgca
      301 ctaggagagg ccctgttttg gcttccagtt acacttgggc tacctctcat aacctggacc
      361 aaccactggc tactttactt gacagagatg acccatgtaa attaactgct caaataatac
      421 ggctcttaat ctgtcatttc atctaagaac tattcatttc attgtttttt gctaaattgt
      481 tgttgtgata cattgtcctc tattttgtct tttaatggaa aaacagaaca tctaaaatta
      541 gcttcagaaa atggtgaaag agaacaaaca aggcgatcca ttagcgagga tgggttattg
      601 gctgacttgc ctcttctcat ggcggcccat taaaagtggt aatgtctgcc tgcagcagtg
      661 cctcgggaag aagaaggagg ccatggaagg catatttcac atgcatcaga cactggaatt
      721 aaatagacca atgttcccta aaaagactga agtacatctc tttctgatgt gcgtcccggg
      781 caaaaattca ttgatcttag aatgtccctt ggcaattata aaatattaaa tccaagtcgc
      841 acttttcggc tcggcttaat tttttttttc ctcgcttttc actgctttcc atttgaccaa
      901 tttggaaaaa aaagctagaa atggatggcc atgaaaaaga ggcactgata ataaagactg
      961 tgcccacttt tagatatctt ttgttatgtt tctgtattgt ttttgtggaa catctcagat
     1021 cattaaatgg gacgaggtgc cagcaaagca gagcgtgcct aaaacatgtc ctattacagc
     1081 atcagggcag tgtaaaccag gaccataaat gagtgggatt gaagcacaca gtggactgaa
     1141 atttgtatga tattgttatt cctggtacac aagtacttct gagccactga tgcctgtttc
     1201 agcactttgg tgattacaaa tatttgtcag ctgctttcca ttaggtctgt ggccccggtg
     1261 ctcgcctccc tggccccttt atgtccctca gagattacag aggggtctac agttaattgc
     1321 ccaaatcatt aaatttaaca tatatctcaa taaatataat cagccagctg tttgacaagg
     1381 tacacagttc attaagtccc cttgcttgtt tgtcgtttcc ctaattaaga tttaaaatcg
     1441 atcattgagt gcgtaatgta cagtgtagct cggcttgtac ggggagtctc t
//
LOCUS       KCVB01000048             896 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2067pri ultra-conserved element locus
            uce-580, sequence.
ACCESSION   KCVB01000048
VERSION     KCVB01000048.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2067pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>896
                     /note="ultra-conserved element locus uce-580"
BASE COUNT          187 a          306 c          139 g          264 t
ORIGIN      
        1 tacatccatc atggcaggaa aaggaaaaga gaatttccac tcctaaagtt ggagcactga
       61 attcctgttt ctttcattac ctatgtcttg ctctccagtt gtcttcctac ctgtctcatc
      121 tccagccagg tctataccct aactcgtgtt agacactttc ttcagcttaa tactgtcttc
      181 agcattcaaa agttgtatat ttctgtctag ctcaacccct gccatcctct ttctcatctc
      241 agttgactct ttctcccagt ttttcctcat tatcacttca tcttttctac ctggccttct
      301 cctcctccat ttctcctatc cttatccctc gtctttatct ctgttttagc atagcattct
      361 tcttccagct atggcacctt aatcacggcc tcttcccggc ttcacctttc tcccacctta
      421 gcccggttca cgtgtccgcc ccaccctccc accttcatac ttttttcccc ccagcacccc
      481 ccggcccgtc atccccctaa cccccagcct cagtgctcct accccggcct cggctctccc
      541 tgtccctgcc tctcctccag cctgccttcc cctctattct gcctccctca gccccagcct
      601 tctgctcagt gttcaagggg agagccagct aggaatgctg tggctctaga cgttttgtcc
      661 aagccctagg tctctttcac tgaggcaaga ttagttcata caaaaactca acaatacacc
      721 actgaaagcc attgttgata ttcaacattt ttcacatagc ggagcagaat agacaatgcg
      781 gcagccggga aagcccgaag cggactagca gggttgatgg ctgatggtat ctcagctgag
      841 cgagggcctt aatccctttt atacacacac atacacacac acacacacac acacac
//
LOCUS       KCVB01000049            1684 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2112pri ultra-conserved element locus
            uce-151, sequence.
ACCESSION   KCVB01000049
VERSION     KCVB01000049.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2112pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1684
                     /note="ultra-conserved element locus uce-151"
BASE COUNT          465 a          386 c          328 g          505 t
ORIGIN      
        1 ctaacaggct tacaaggctc aagggacaac aatttacaat tttctttttt cttatttttt
       61 tgcttttccc ttttatcatc atcatcacca tgatataaag tatatcagct tgttatgttt
      121 atgtttcaaa gcaagacttt ctaacagcta ccatcgacag tttgcatttc accctctgtg
      181 atccgtctgt gcaacgacgg gacactaaca agctaccttg aattattctt acttgtgtgc
      241 tattatcaca tggcctgagc atctaacctg ccctcgtgcc cgtctgcatg acagacaaat
      301 aaagtctgtg ttaggcacat gggagatgcc gggccagata accccacatt tacattcaaa
      361 cagagctttt gacaaagcct tagcaacagg ctccttaatc agcgggctgg ctgcgcagcc
      421 ccggcctgcc ttctcctctc ctctccttac agccctgatc ttgagaagga gctttaaatt
      481 ggaaattatt acacattaaa ccctctgcgc acaggccagg atcgaactgg atcaatatct
      541 tgcccagcac aataaatcag atgctttctc tccccatctc tcttttcccc tttgcttttt
      601 caagtggcta taggccactg ctcgactgga acatatttaa attttcttgt aacaaattaa
      661 ttccaacatt ttctcttgat tgaattaggg caagtgctgg tgctgtcaca agtgtttggg
      721 ctgctcctgg ggagagacaa attgccaggc ctggacacgg gtaacaagcg gtggtgttga
      781 gtcgctgata atctccccac taattctcca ttacagcaaa atgaggccat aaatcttcct
      841 gacagctgat cgatgtaaac attgtttccc ccatgattgc cagctctccc agcggagcta
      901 ctgtccgctc acaatccgct gaaattactc cggcaccagc cctccctccc ttcccctgct
      961 ccctggaagt ggtgtgtgtg cgtgttggac tggcctggtg gggaaaccaa agtcagtgct
     1021 gtcctgtcga agtggccatc taattttagc tcaaagctta ctaaacaaat ggaaaatatt
     1081 aatcaaaatg tctcctgttt aaccactggc acaagcaatt ctttatagaa gaatgattgt
     1141 tctgttacca cacggccaca cggtgttgca ggttatatga gatgaggaag actgttgaaa
     1201 aatgaaggaa ataagaaata gagggtataa catgcagtca aacagtgatt tcttttctaa
     1261 atgcagctat cactcctcct ccttcttctg tgtgtgtaat ggcattaggc ggataaacgg
     1321 cagtgaaaga ttgagttgtc aaacagtcaa agcacaaggc tcgttatcag cgtgttcact
     1381 gtctgataat attctacacc attattaaaa tccaatatgc atgcaattac agagacaggg
     1441 atgctgttaa aacgtgtcgt ctggctgtaa tcacagacag agaaggccgt tttaagacat
     1501 gccactttaa gaaatctgtt aaaatggcat gtatatagaa ttttggaaac ttcttttatt
     1561 cacatattgc aataaacagt ttaagactat aaaactgaaa ttggtttcaa gtaaaaacca
     1621 gagggtacac ttgtgggcag atactgactg cttcattttt tttattttct tttatcttat
     1681 tatc
//
LOCUS       KCVB01000050            1109 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2155pri ultra-conserved element locus
            uce-957, sequence.
ACCESSION   KCVB01000050
VERSION     KCVB01000050.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2155pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1109
                     /note="ultra-conserved element locus uce-957"
BASE COUNT          341 a          249 c          194 g          325 t
ORIGIN      
        1 gtgttttcag ttaaaaaaat gtctatcagc attgtgtctc ttctcaactc tgagtaagat
       61 gcaagtcttc tgctgtaacg caagcgaaaa ctctgctctt taatgagatg tggaaagaac
      121 aggaaaaaat gataactgtc aaaaatgtta accttgtgtt ttatgacatg gtcctgagga
      181 aaacacggtt ccaagataat tagctgaggg ctccaagagt tcagaattag ctgtttatgc
      241 agacttatca tggtaaagtt gtaccaaaga taacctcctg tccgttacca ttaaccagtc
      301 tccttcagag aacttaaagc cacgagcgtg gaaagttgcg ggaactttac aagagctgaa
      361 ttcttcttaa gaacacagac ttctccacag gctcttttca tcagatatca tcatcactgc
      421 tttgacgaag ctcccctcct tctaattagt ctgttaatat tatattgatt cctttcagat
      481 gaagtcaagt gctggcacta atcaggctat tatgaaaact cctccgttaa gattcccatt
      541 ttctttttct ctcttgccat aactttttgt ttgtgtgaac catatgtccc aaaaataata
      601 aagccaggcg gtaaactgag ccctcttccc tctgtcactg cttatcacag aagaattggc
      661 ctcaaactta ttctctcttt ctctctctcc ctcccactct ttccctgtat ctctctctct
      721 ctgttctttc tgttttcgca caagtgtccc agttaagaaa ttgcttacat cccactcagt
      781 ctggggctgg attgaaattt cccatagggt atgtaccaat atgcagagag cacacttcga
      841 cagatgccat cagagaacaa gcatatgaat taccaggcag cctgccatct ttgagttcag
      901 acagaatggc attaatacct gattacgatc caaacaacaa cacaacaaca acatgaaagc
      961 caaagcaact gggagcaggt aaaggtttca tcatttatat attaatggat ttactaacaa
     1021 agactctaca ccctcaaaag ttaaaatgtc atagaggctg ccaacaatca agggactttg
     1081 gctagaaaaa aaaaaatagg ggtaagaaa
//
LOCUS       KCVB01000051            1511 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2203pri ultra-conserved element locus
            uce-761, sequence.
ACCESSION   KCVB01000051
VERSION     KCVB01000051.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2203pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1511
                     /note="ultra-conserved element locus uce-761"
BASE COUNT          426 a          277 c          285 g          523 t
ORIGIN      
        1 ctgtctgtag tactataacc gaactgttga tgttcatgtg atgcataagc cctgtaatac
       61 gcaaacagac ttgctgtaaa tattgtacac tgattttctg tagatggtct aacatctgtg
      121 tgttcatgcc ccgttcccct catcaaaaca aaatgatgaa attcaggcaa gggactaaat
      181 gatgtggtag ttcctatcat tccaattgct atgtaccttt attttattta aggaagacaa
      241 aacattgaag tttgctacaa gggtttcttt agtgttaact tgttttgtta ttgttgtttc
      301 tgtgtattgt actttttact ttttgtttac ttccatattt ttttccctca atctcttttt
      361 ttgttgctcg tacttggtgt tgtggaaagc aagacagaag ggaacattcg gtctggccta
      421 tttcctggtt tttcggaact gagacagcca atcagatgag caggattctc ctttagtttc
      481 tccgctggat ttccatgttg ctgctatgtc ccagcatcct cactgacatc aagtgcaaag
      541 ttatgtgttg aagcttctgt aaatacattc agatgtggaa atacaagctc cctcgtaaga
      601 aaatgtacat atagctcacg caggagaagt ccatcatggc caggggacaa aataatagtg
      661 tttttataaa tataaatata tattgggttt gacaaccttc ggactgttac atgcactttc
      721 ttggaaagtt ggaagatctt tatgggtcca gtttctctac actttaatac ctactaacaa
      781 ctaagatact gtaattcttt actctaataa tcaaatacat cagttttcca aaagaaacat
      841 tgtgtgtttg tgtgcgttta ttccctcttt tatattttat catatcattg aaattttcaa
      901 ttattttttt tacacatcaa tcatttccac gcatttaaaa aagagcagta ataactataa
      961 aatacatata tttacttttg cctttgaata atttgtgttc atggccgttg tgcagcctgt
     1021 gcaactcttt ccggtgttta tacactgacg aacagctgag gcagaaacgg gggcttgggt
     1081 gcagtcactg atggtgtgag cttctttgcg ctttagttta aatcatgatc gcagttttaa
     1141 cctcataatt tccgttgcca atccttgaaa tcgtcaatta atcactaatc ctcggaatca
     1201 agtagattca agacttttta cgcgggaggg aaaaaggttc cctcctgcca ttcatttata
     1261 cacgttgaaa gacggttaat aataataaaa agtctcagcg gagagggtgt tattttgcct
     1321 ggacacgaaa gttaagtagg cacaatttag cacgagcact atgaaggtgt tagtgatgct
     1381 ttttggaaaa tatgcgtttt attaagaatg cgacgatcga attatttgct acaaagcccc
     1441 aaaaaaaagt tgtcaaaacc acccggataa agcctttgac gggtctgaag gcatttgcct
     1501 tgcatttttt t
//
LOCUS       KCVB01000052            1828 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2243pri ultra-conserved element locus
            uce-48, sequence.
ACCESSION   KCVB01000052
VERSION     KCVB01000052.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2243pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1828
                     /note="ultra-conserved element locus uce-48"
BASE COUNT          528 a          416 c          449 g          435 t
ORIGIN      
        1 gaatggaggg ggggggctct tgcaaggcag aaatgtgatc cggagcctgt gtcgcattag
       61 ccttaacggc cgtgtggcag acgcactggc accacagggc ggaggagacg ggtggtggag
      121 gaggaggatg acgactggga gcaagacgag ggaggaaatt cgccggacac cctcacgcag
      181 gaaaagtatg tgaagctgag gaagaaggaa ggaatgtcat catccccact ggaagacaac
      241 agggagggag aatgatgctt atccctcgat ctgagtcacc tttgtaccca cagcacacac
      301 atcatcacac atcccctgtc agcctgtcat ccaacagggt cacggtcaag ctggcgctcc
      361 ctctctctct ctgtgtcatg ttgtctctcc cctgctctgt aatttccttt ttttttcctc
      421 tcctctttcc cggaaagaat gtgtctcctg tcgctccacc agtcagtgtc agggggctaa
      481 acagcccatt tcactgtctg actcagttac ggagtcctag aggtgcctga aggattccaa
      541 gaattccagc ttttgcaacc ataactgtga cgccacctga gggaatgccg cagagatcat
      601 accaaacact tcctgtagac acttgggatc catctttcat ccagcagcaa catgaatctc
      661 tgttttcaaa attaacaaca cgggtgataa acaaaattaa gtgcatgtgt ctgagcggta
      721 tattgcacaa aacaccaatg ctgtgcacat gcatgactgt gaatggaaga cagagtgatg
      781 atgacagaga ggatgaagga gcaagagagg aaaggagaga cagagcctga gtgagaagag
      841 tggagggggt aatgactagg tctgctcagg acagaggagg cgacagaggg ggaagcgtct
      901 ccgatcgtct cctcgtggct cctctttggc gtctgtgatc ctcggaccct tcacgcaatc
      961 tcccttatta caaggcttcc cctctccaca cgggcaccac tacaaaatgg agcaattaca
     1021 tttaattagg ctgcagaggg tagctagcct gccccatccc aaaacctcag agaagcgcag
     1081 acttcaggta acacaacggt cggggagaac ttcaaagaga tgcaaaatta cagcgccatt
     1141 agtggagcca ccacagaact gctgtgaagc cagggaatca tttgacccag agaggtagat
     1201 tcaaaggagt taaaactgaa aagcagagca ggggagacaa aaacatctca acagccacag
     1261 attaaggggc agactgaaca cttctctaaa cattgttttt gttgtatggg aggcttgaaa
     1321 aattaagtct ttacaatacc tattagttaa cccatatccc tctagcagtc tctttggaag
     1381 gatgctcttt agggtcatgt caaacatttt gtggctttac agtaaaaaaa aagacatttt
     1441 tgttccaaaa caaacaggat taagtgtcct agtaagtcat tcaggctgtt ctggaaagat
     1501 gatatgcaag gcaatcccac tttatctgcc tgttgtttgc agcgctggat tttggctcac
     1561 agacaggcat gatggtccta aaatttttat tgatgctgac tcactagacc tccaaccaga
     1621 gtggaaaatt ctgacttaaa tgctgttaga tgtgcttttg gagacagaag gactcttgtt
     1681 taaaggcaca acatccaaac aggtgagcta acaaacaaaa accactcatt ctgcattttt
     1741 ccaatttttc ctgtcggttg taatgcaatt gaggagattt aatcaagtgg tgagtcaggg
     1801 atttttgcaa taaaacacac acagacac
//
LOCUS       KCVB01000053            1330 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2318pri ultra-conserved element locus
            uce-582, sequence.
ACCESSION   KCVB01000053
VERSION     KCVB01000053.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2318pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1330
                     /note="ultra-conserved element locus uce-582"
BASE COUNT          448 a          237 c          254 g          391 t
ORIGIN      
        1 gtcttagtac gcctcctaaa gcaatcaatc cacagacacg caggagccaa atattcatta
       61 gtgacttatt catattcaaa agatgccttt tgaaagtaac aaaagagcag aattgtcttt
      121 gacaggcaca ttacaatgat gctatgacat actgtgtgag gcatgaacaa ttgtgatcat
      181 cttgtattat tcaatgaggg aaaagttgtg ttcattattg tcgtctgcca catgagtaaa
      241 tctgtattaa caactagatt atctgacaaa gattatagtg ttcagaatga tcagaatcag
      301 acaaacgttg cactaagaaa caaacacaaa cggctcgttt gatgccacaa acattcaagc
      361 cagaattttc cactcggatt tgtttatcct gacacaaagg tcagcaaaaa tatctgcgtt
      421 tgcacaacat agacttgtgt gagatgtttt aataggaaga agctaatagt ttcacacaga
      481 gcgattcaac atgattaata cttgtgggta ttgtttaact actccagccc attggccctc
      541 tgtaactggc aaaaagagag agagatgggc tgcgatgtta ataattagtt ggttctgcgc
      601 tcaaaaaaac tctgtccaac ctcacaagat tttaatcaaa ggctgaaaat agtagctgat
      661 tgtaatcggc actcagaagt caccaggggt gtttgaagag cccgtcaagc ctgagagtgg
      721 agctaattcg attaagtggc ggctgtacct ggaggggacg gatctgtatc tctccctttt
      781 ttatttatga gattaagtcg cgcttgttat tccaatataa ataatcaagc caacagaaat
      841 gacactgatt ttcaagttgt tttatctcac ttgtctccaa aagtttttgt ttatttcagg
      901 tgacaagtaa actttgttga gtaatactcg agcggtactc atgtaacgaa aattgcggct
      961 tcgccttcgc atattaccta acacagtgtg attgaaccaa aggaaaaatc aaagtacagg
     1021 cactagaaaa aaatgtgctg tattatttat aatatttgta caatttcgat aaattttgag
     1081 gatggatttg tgcaaacgaa aaacaaaaac gtgacatgtg gtaaaataat gatgcagaga
     1141 cttacaaata gttaaagcac acattctcca gactaatgtt tgaaataaga tcatcagttt
     1201 cagcaattct tgagatcacc ttctcaaaac tattctagtt atattaaata cacgcagaga
     1261 aaagtgaaga cattgttagg tcacattatt attactctta ctaaagatga aagcacatgg
     1321 attcctctgc
//
LOCUS       KCVB01000054            1829 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2364pri ultra-conserved element locus
            uce-910, sequence.
ACCESSION   KCVB01000054
VERSION     KCVB01000054.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2364pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1829
                     /note="ultra-conserved element locus uce-910"
BASE COUNT          501 a          459 c          348 g          521 t
ORIGIN      
        1 ggggaagatt agaccaaaac cagagcagga aaattcatcc cacctgcttt tgataaacct
       61 tttatccttc atttcactct ggcactttct gtttttcagg gtgtagttgg catgcaatgt
      121 ttagatttaa agtgaaatgc catgacacaa aaacactcga gcttagcatc ttttacggct
      181 ccattatatt tttatgttga gtgaagtcga aaatagacac aaacgcacac acacactgat
      241 gaaaaataca acagcaacag acatgcacat tcacagagat gttcaggctc tgcagttaaa
      301 cgcacttaca ttggtgcagt acaagactcg ctgtggtcaa agcagactgg accgctacta
      361 ttgtttttat cccttttgtc ttttcagtga ccccccttac acacgctcaa acacatgccg
      421 gcacacacat gcgcacacat cagcttatac ataaacacaa ccctgttgca tgcgcccaca
      481 cacatgcaca cacgtaaaca cacacacata cacataccag catacatgaa cttctggctt
      541 catgtttaaa gtatgcagtt ttttgtgcgt gcgcatctgt caaaaaaagt gaggggagga
      601 ggggaaacag gcaaaggaga agggaaaagt gggacaagag agaatcagag aaagagggag
      661 agagaagttg agccacagaa caaggctgct gcgagcagct gtggcactcc cagatttccc
      721 cctgattgct ttcagctggt acttgaaccc tgggcctttt gggttttgga ggccagccca
      781 gaccccacaa tccccccagc gccccatcca gctgcctgac ccctccctga ccccgtgccg
      841 aggataggaa aatacgagcc gtgccaagcc agagagactc ttttattttg cctgctggca
      901 tgctgccggt gaccagggct tacataatgg gaaacatagc aggacccagg agaaagcagc
      961 ccctgccccc ctttctcttt ttccttacca atcgatcctc ctctttctcc cccatctcct
     1021 ttctcctctt ctacatttct tttcccattc tgctcccctc ttgcccccac ttgcttcatc
     1081 ctcttccttc ccctgtgcac tctgcctccc ctcctgtttg tgatatatct ctttgggccc
     1141 actcgcctgg gcacatttat tacagacagc ctcgacacaa tcctgaagcc gagccaccag
     1201 caacacaaca gcacagcgca cgcacacaga cacactcaaa cgcacaggcg cacccacatg
     1261 tgggcgtgtg tttgcacact ctgaggcatt tacagcttgt gttgtgcatc tttgtgcaga
     1321 gaactttaat ggaagaatgt acatgtggta attaaatgtt acccccacat aaggacatag
     1381 agaccagcca tacattattt catataatag tattttttta atgtttttgc catttgtttt
     1441 atgactccag tatggttatg tgatgccttg aggtttttgt tttgctttat tttagctcaa
     1501 taaagcaaat tcctatcaac cgcattgaaa aggcaataaa gtcttaaatc atgaatgagt
     1561 atgttttctt caagaagcac tagatgttca ggatcagtta tatctaatcc atatccactg
     1621 tagcttcact ttggtaaagt aatgtcaaat gtgatggttc atgtttgaat ctttctttta
     1681 ctttgtaatt attttattta tcatactttg gatttacaga tcacgtctgt tgctatttat
     1741 tgggtttaat tttatgaccc tacttattag aattcatttg gaaatacaat acagtaccag
     1801 taaaaacaag cacttggtat attagtgcc
//
LOCUS       KCVB01000055            1654 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2411pri ultra-conserved element locus
            uce-153, sequence.
ACCESSION   KCVB01000055
VERSION     KCVB01000055.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2411pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1654
                     /note="ultra-conserved element locus uce-153"
BASE COUNT          440 a          376 c          396 g          442 t
ORIGIN      
        1 atttgaaata aacagcacat tttcccattc cttacattat ttcggtcatt ttgttgtaac
       61 caaattatca cactgttgtg ctgtttgttc tgcaggttga cacagcaact gacatttcat
      121 acacccccat tctatacaat gtgcatccta tttgctgtaa cacactaaca tgtcaaacag
      181 ctctgtcctg taaaggctgg actctgaggg aagtagccta cctggtatgg aggtcttcca
      241 catgtgtcca ggctggggct gctccttcgt gcagtaaacc tggccgttcc agccgttggt
      301 ggcgagagtc cacggttggt agctctccat aggcagcagg gactcgtgtc tggcctctga
      361 cggcgcactg atagttggca caacaggaac gtccaggtaa ctgggaacag gttggtatgg
      421 gcctgaaggg taggtcggat aaaaagcgaa ttcctttgcc cgcgaggtga agtcgtcgct
      481 cgaagcggac gtgtccatgt atttctctcc atatgcggag gggggctgcg ctccacacga
      541 ctttatgctg ctgtgatgcg acattctgca cgggtaatag ccgctgccga agtacccata
      601 tggcagggac gcgctggatg aattctgagc tgcagagcaa gggctgcact gtttcactgg
      661 ctcgcccata ccggaggtgg gcacgtcact ggacgagtaa gccgtgcttg gtgccaggga
      721 cgcggggtgc gccatcagat tcctacactg gttcgcagca aagttgccac caacaaaacc
      781 ctccatattc ttgctcactt catcggaacc gccgccgttg tcgtagagga acatcaccga
      841 cgggtcgacc cagcgaggac ggaggagcag tgacgttgtc atagcacggc atccaacatt
      901 gaagcttgtg tctctttttt aaaaagcaca gagactgaaa aaaggagata ctggttccca
      961 gagagtaatg ccactctgac gcgcacaaga gcgcagaggt tgcatcgtca ttggcccaga
     1021 ttgtatcacg tgaccgggat atgcaaagag gacggtaaac atcacccagt acagataatt
     1081 aaatggagaa aaatgcggcg aagtaaggtg accgtacata tgaagggatt tcgctttttg
     1141 tcgcatttgc ttcaatttag atggacgttc tgccttttta aaaagataca cataagagca
     1201 gcatactttt gattcatata aggttgtttt tcagaattta aaaatgcttg aattggaaaa
     1261 tattgtcttc tttttcttat ttttatgtgt gaggttcaaa taatagtcta tcactgaaac
     1321 gtgttcagca attaaagaaa atatctgtga ctcaaccatc tacagtctac cttatggcct
     1381 tcacgcacgc acactgacca gcctttgtct gcgtcgccgt agaaagtggg tatcattaca
     1441 caaccattta ccaaaggaga tttacattta cgattacatt atgttaatat gttgtattta
     1501 attgtggcac ttgatacaca agggtcaaaa cgtactcctg tcacctgcta atgcatagat
     1561 aaagtatata gtatgtcata ctttccaatc cgtactatgt accaaacagc gcgaccagta
     1621 tgggatgtaa aaacgcttta gaaatgtatg actc
//
LOCUS       KCVB01000056            1491 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2452pri ultra-conserved element locus
            uce-955, sequence.
ACCESSION   KCVB01000056
VERSION     KCVB01000056.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2452pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1491
                     /note="ultra-conserved element locus uce-955"
BASE COUNT          472 a          274 c          383 g          362 t
ORIGIN      
        1 gagattcagt gtgctttttt agtaaatgag gagataaata ttgcatcagt ggggaatagg
       61 acacagcatt aggcagcaaa aggcacaatt cttgcaatga tgcatccttt gagatgggaa
      121 ataatcctta acagtatttc ctccggattt gaccaggtgt caacaaaggc tgcacaaaaa
      181 ctaacatatg aatcaggcaa tggtattttt tttctatata tctttctttt ggccttcctg
      241 ctttttgtgt ttactgctgc aatacctcat ttctcggcta caaaaggaaa gaaagaaggt
      301 gaggaaatgg agggggcagg agtacaccag ccttattatt gccttgtgga tgaaggatac
      361 aatcactgct ggcatttcta caagacacac agccaactaa cacatatttt aaagtgaaag
      421 agaagtagga ggaggtgtga aggagagagg gaggcaggcc cgagagaaaa aagaaaaaga
      481 gacgcagaca gacaggccaa caggctgaca gagacttagt gtcagggaca gagaggaaga
      541 gagcaagaaa gaaagtaggt gatatagaca gcaaaatgag caagtgatag aagtgaagtg
      601 agagtgagag caagagagaa agaaaaggag gggcgacctg agagaaatca aacatgggcg
      661 ctgtgcaaca gaggtacgtc ggcaaacgag catgttatgg cagcccgctg ggacttcccc
      721 ggcagatcgt ttgtgctgtt tcccaatctg ttctgaggag cggcgcagcc ccacatgggg
      781 acgtgtgaag gcgtcaagat gggattgctt aagacagatg aaacaaggtg ttttttgatg
      841 ccatctcgag cggacaaaag cctgatttcc tgttttgcct gtgacacaaa gggtgctgtg
      901 tagtttgttg aacagttcaa ggctttttgt tgtcagggct gtaaataact aaacacaaga
      961 gaggtgaagt tacagaggtt ctggatccac atgccaaaaa ctgaaaaaaa tatgtggcac
     1021 attttccctt tttttttggc atttataaac acctgtaaca aacaaacagt attacagttc
     1081 tctcatcagt tctttacata tttcccttca gtgtgaaaac agcctcttaa gtgaatatcc
     1141 tggcacacac ccatttaact ccggacggcc acagctctct ttacattcac tggacccctg
     1201 aaacaatccc atagtgacag agatcaaagt gtcaggcttt gtaatattgc tttacatttt
     1261 aaagggcgct gagcagccag gctaaggttt ttacaaggcg tgctgagtcg ttggggaaat
     1321 aagggagcaa tggaggaggg aatgaaggaa ttttaggggg tgatgggata tgtctcactc
     1381 tttgttcgtg tacacacaca catacactca caaactgaaa tcataccatg tgcagcggaa
     1441 gagcaggcgg tgaggaaata gaagctaaat ataaagtgac acgctgcatt c
//
LOCUS       KCVB01000057            1041 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2492pri ultra-conserved element locus
            uce-116, sequence.
ACCESSION   KCVB01000057
VERSION     KCVB01000057.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2492pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1041
                     /note="ultra-conserved element locus uce-116"
BASE COUNT          343 a          199 c          204 g          295 t
ORIGIN      
        1 caggtgatga tattgagctt catcagttgg agcctgtcgg tgaaatcata cacacacaca
       61 cacacacaca cacaaaacac acgcatgcac gcacaaattg tgcaaaatgc atgtctcgtc
      121 tcaggtgtgt atgtttccaa atctgtgccc aaatcctcct cttcctccat ttcaataaag
      181 aaggtcattt gctcagcagt gagcggcagg attttcctca accttatatg ttgtgtccag
      241 gtgtgtgtga ctgatgtagc tctgcgagac gccaccgtgg gacagatctc ctcctgagcc
      301 aacttaactt atcaggaaca tggaccaaaa aatcaggcta attaattaag aacagtacta
      361 gaattaatgg gatattataa tataaattgc tacattctgg aaccaattta atccaattac
      421 tggaacccct catgaatata atacctccaa aatcacttcg tcagagtcca aagggagatg
      481 tgtctgacta ctaagactga aagaaaagga aaaggaaaaa aaaaagatct gctccctttt
      541 aacggcgaac accaatgatg atttagatgg ctgagaggaa ctgttaattt gagtaataaa
      601 cctaatgatg attttttttt ttttctaatt gacatttcga ccatttacaa cctgagtagg
      661 tttttgtttg agctcccttg tgtttgataa aaatgaatga ctggaaaggc tgattggtgc
      721 tttttctctt aatggcctct acccaattta aaaaaatatt atcctaaaga gacaaaagaa
      781 taccattctc cagcatttaa agtggagtgc ctttttggag gagtggatgg gataaattga
      841 catgaagaat cacagcaact ttataggctc aggaatcatt tcatgaggga taaatccgca
      901 ttacagcata tggactgatt tagagtcagg tggaaagact cttgctgagc atcttgtaga
      961 tctgaacaaa tcgcaaagta gtttggaagt aaaatgtctt gttattacaa ggtaaatgaa
     1021 gcaaacatac cccaaaagtt c
//
LOCUS       KCVB01000058            1672 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2532pri ultra-conserved element locus
            uce-252, sequence.
ACCESSION   KCVB01000058
VERSION     KCVB01000058.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2532pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1672
                     /note="ultra-conserved element locus uce-252"
BASE COUNT          431 a          365 c          344 g          532 t
ORIGIN      
        1 cagattcaga tcctgtggat atttttcctt gcacattatt aacaaatata aaagtgcata
       61 ttttaatgtg acaaaatatc ttggattttg tttatttacc aaatgcaaac tgtattaata
      121 gcagaatgca tgagtggata aataaataaa aattagcaga taactgtcaa aagctaaaag
      181 cagcaccatc actagcttaa aagactgttt gagtcctttt gaagtgctca cacatcatat
      241 gtttctgctt gttttgacta cagcagagag tgtgtgtgtg tatgtgtgtc cgtccatgtg
      301 tgtgtgtttg ttgcatgaga tgctacattg ctttggctca ctttaaaacc tttggcacaa
      361 acgggacatt ctttgtctaa agttagaaga ggcctggctg ttaattaatc catcgtaaat
      421 tgattttgat ccctccagtg attgattgat gcttctaata cacactcaac ggtgcagagt
      481 cctccgagga aagcaggcgc tgatccagtc tttaagtttt tggctagctg tcagaccgcg
      541 tccctaacaa ctgacgagag tcttgaaact atatagcaga agccgagtac gtatttgcat
      601 tttagaagtt cgcctgtgtg cggcagaaaa tctagaacaa ttgttggcct gtcacttcag
      661 ctttatctta aaaacttaca aaggttttcc tccttgccat acacggctgc ttgggtaatg
      721 ctgcctcctg tgtctgcaga cgtatttttc taaaggtgtg tgtggcacat ctgtgcagct
      781 attgtttatt catggagtaa accctttgaa atgtgttaag atacttccca ctcattataa
      841 aaatcctgcc acccatggat taggactttg atgtcaccaa tcctgcttca aaggcttctt
      901 tcactgctat taatcacagt ttagcctctg aagatatagc cagagaaaac atttggacat
      961 cttgtgcaga aactttgaaa tgttcagact gtcttgcata tgctatgttc atatcctttc
     1021 cgctgtgtgt ggggctgttg gactgctgtg cacgcagctg cactcctgag tccatgggat
     1081 tcagctagat ttaggctcct tctgaactgt ctttcatgct gcagccgtat gaacacaact
     1141 agtgtagctg ttcactttgt ttttccttgc gtcttgtgca ttgaaaaaga atataacccc
     1201 aaagtgtata cgttgtctcc atgcttgata gttccctctc agtaatcaca tcttccctta
     1261 agttcacaca gctgttgagg ctccctctaa gctttaaata aaccctgaca tggttacata
     1321 cttatgactg gttttggaca ggaaacctgg cgacgtccag agcccgggtc accgctgcct
     1381 ccagatctct gccaccgcag ctcggatcag gacgcaccaa ggtgagactc gcgctcctgt
     1441 agcacaaatc atcacatctg agaacataat gttttttatt tgttgctcac aggcattgca
     1501 ccattagacg cctagtaaaa acctgcgctg tgaagcatag ccgcattaat tgactcccct
     1561 gttatgtttg ctgtcttgct tgtggtgatg cagcatcgcc atctggcatt cggcgtctac
     1621 gacataaaag tttctcagac ttactaacta tcgttgtttt gttgttaatg gg
//
LOCUS       KCVB01000059            1097 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2576pri ultra-conserved element locus
            uce-1225, sequence.
ACCESSION   KCVB01000059
VERSION     KCVB01000059.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2576pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1097
                     /note="ultra-conserved element locus uce-1225"
BASE COUNT          230 a          233 c          383 g          251 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtctg tgagtgtgtg tgtgagagag
       61 agagagacgg gggggtgggg gtgaattacg tcggcgtggg acagtggagg gacggtctgc
      121 tcattgcact cagcttgcag ctggaggaga gctgcagaga ggggaatcaa ctgaacagcg
      181 gcgaatgaga ttaatgcaca ggtaaaaatc ggtcgaccgt agttggatgc atgagttaca
      241 catgaaattg tttgaacttt tagattagat tattgtttcc aggtgggaat agatctgcag
      301 tggagggaaa gcaagtcaag atgtgctgcg tccgtggcgc caccggtgcg ctatctgggg
      361 atagagtgag ctacggcgac gcgctgacac cccgctttta gatggctcca acatttgggg
      421 tgcaaaacag gtgtcaggga agtgtcgtca cgaaccccgg aagccggagg aggtgggaac
      481 atcagaggga aagatggcag cgaaatccga tggtcgcggg gtcgtcaccg gtttcgccca
      541 gctgcacaac ctggacgagg tggtggggac gggagaggac gacactcgga acggcagctc
      601 attccacatc tgccattgct gcaacacctc gtcgtgctac tggggctgcc ggagcgcctg
      661 cctgcattac ctccggggga aggggaagag aaaggggaaa gagagagatg cggcgcggcc
      721 accgcaggag gagcgcctct ggctggactg cctgtggatc atcctggcac tgctggtctt
      781 tttctgggac gtggggacag atttgtggct ggccatcgac tattaccaca agcagaactt
      841 cttgtggtca ggcctgaccc tgttcttcgt gctggtgccc tcggttctgg tccagatcct
      901 gagcttcagg tggtttgtgc aggatttcac tgggggcgga ctgggctccg tggaggggct
      961 gagtagtcga agagctacag ccggtctgca cagtgacagg tgctgccgcc tctatgtctg
     1021 ggtctggcag accatcatac acatttttca gttgggacaa gtgtggaggt aagacaaact
     1081 ttaagttttt cctttga
//
LOCUS       KCVB01000060            1669 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2620pri ultra-conserved element locus
            uce-1260, sequence.
ACCESSION   KCVB01000060
VERSION     KCVB01000060.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2620pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1669
                     /note="ultra-conserved element locus uce-1260"
BASE COUNT          563 a          348 c          323 g          435 t
ORIGIN      
        1 ctttaagcag gaatacataa tgcaaaagtg tagacaggcg gatcacttct agcgcccatc
       61 cccttttcaa atagctgcct aatcaatctg gcagacttga aacagtgagg taatctacaa
      121 taattgaatg catagcacta tgatacacag tacagaaaat aaggaggcaa atgtaaagcg
      181 ctcccttcct tcgatgacca atttgcagcc atcaaaaata agggcacatc ctttcaattt
      241 gtggctgtat tttgcccaaa aggacacagg ggtaaagtgt aaaattggaa aaatgatttg
      301 tatttctact ataacaatgc ctgatcctga aaacggcaaa catgttaaca taaatgtgct
      361 ttctatgtgt gttggttgtg ctagaaaagg actaaattgg tgatgtgcat gttagaagtc
      421 tacataaaaa aaataaaaga aatccctgaa tgcatcgtag tagaaaatag ttatcttaaa
      481 ttacagattt aaaacggtaa ctgattttta atctcaaacg caacagagac aaacagctgc
      541 aattgggatt ctatgtttag gaaagtcaag taacctgtga aacatagaaa atgctccaca
      601 gtttaaaatc ctagaacaaa aaagagaagg tatctaatgt ccctctcaaa atctccaaca
      661 ttttggcaat ccaccctgct atttcagcta gagcgaagag cattctagta aggcatcatc
      721 atcatcagac actcccatgt catgtaaatg ctctatgctt tatactcata tggagaaggg
      781 ttacgataga gtagtggcag tgagtacatc tctcttgcag gacgtcccca ctccgacaga
      841 tcaggtgccc ttcctcgtcg tccctcacac tctgtgcccg cgtccggctg ttgctccgct
      901 gttttcacac ccaaaccgcc atcagcaggt cacgacacga ccggaggtaa cagagggttt
      961 tcaggggcag ccgcagccac ggtgtcaggc ggcacaggta gacgaggagc aaggaggagg
     1021 gtggtgttgg tggtagttga tgtagttgtg gttgtaggtg ggtttggtgg tcatggggcg
     1081 attcacgcat gacaccacgt gaaggaaata tataacgata gggatatagt gcaagggaac
     1141 aagtcaaaga tcacatgttg tcctctgctc ctctcccccc tctaccttta aaccacagct
     1201 gcttacaaac aaggaagcaa ccggcatatc cattcatcaa cgcaaaaacc aattaagaca
     1261 ataagttact cattcaaaac ttattaggtt gacttttcag attaaacaag atggaaaata
     1321 aaatgagagg accatatgta atcaagcagg aaatatataa agacaacccc caaaatatat
     1381 actacatcaa actcatcctg tggctcactt aaatttatac ttaatacacg tgtggttaaa
     1441 cataacccac acaaaaaagg cattttaaca aatctaagac attttaaatg ccgataaatt
     1501 caacaacaaa aatcagcctt caatttcatc taaatttgca tggtgtttgg tagaacattt
     1561 tcactttttt ttcagcctat ctgcacttaa tatcagaagt ggccagccaa ctgctacaac
     1621 aggcatttag ttcttttata aaccaactaa gaacatacaa ataaaaatt
//
LOCUS       KCVB01000061            1844 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2696pri ultra-conserved element locus
            uce-462, sequence.
ACCESSION   KCVB01000061
VERSION     KCVB01000061.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2696pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1844
                     /note="ultra-conserved element locus uce-462"
BASE COUNT          499 a          461 c          377 g          507 t
ORIGIN      
        1 agcattaagt actaacaact gaattacaca catttactct ttactgtgtt aaccatccat
       61 atagtgtgtt tacatttctt tctctaaccc agtggagcaa cctcacccta accatgccct
      121 ctgctcagtg tattcaccca cactgctgct atgaccagcc acactgactg acggagagtt
      181 cagcaaaggg acaagaacaa agagcagatc ggagctcgca gcagatcaca gatgggtggc
      241 ggtaagcaag gcaagccagt cagagattca cacaggccac ccaaccatcc agccatcaac
      301 cgcagcctct gcagggccag gagctgttga aaatggatcc gtcacaggaa acaggcagac
      361 agacacgcta gcgctgatgg aggtggggga agtgaggggc tgtagctcca ttatttcagg
      421 aagctgatat tccagggagt taaagtattc ccttctctat ataattataa gagccaattt
      481 ccctttttga aataatgtct tgctaaccaa ttaattgtaa taatcctctt tgtggaaaca
      541 atggcaacac aagacaagat gacttgagga tgaaaggaaa gaaaaaggag atctggaggc
      601 ccgcacatat aaaatgtgga gcacgggtgc caagtgctgt atttttaaaa gcaatgtgct
      661 cctcgtttct catgcaaatc accatgttgg accagatatt gccatcaata agtttcccac
      721 agatacagta gcaaggcaat catttacctc cattgacagg tcaaaacacc tttcattaga
      781 gttacgaggc atctcatcaa gatgcggcca cgctcgcagc tgtatgactg ctgtgtattg
      841 cttaagtgtg tttcattatt cccagagcaa cgttgatgat ggcgagggaa aggattacac
      901 gtcagggtga gtgattgggt ttgaacagag ctgctgcata cactttgtat caacccaggg
      961 ctcaaattac acttaggttg aaaacggagc acgcaaatgc ccaggagagc ttgtggcaca
     1021 gaaatgaaga gtgggccacc atctctctct ctctctctcc ctctctcttt ctgtttccct
     1081 cacacacaca cacacacttt tcatctcttt cctttgtcaa attttctttt gaccccccct
     1141 tcttccctcc ttctcctcct gccctacgct ctcctccttg tttttgaggc acaaccatcc
     1201 tttatgggca ttcttattgt tccactgatt ctgttatgtg tatttattgc tccatttgtg
     1261 aaattggagg caccggccga caagcctatt actattcaat aacaggagct gggcattttc
     1321 acaaccgtga gctatcaatg tcccagacag aaacattacg tcacatcaat ttcaaacaac
     1381 agatagattg ctatattgtt gctgtgtatt gtattggacg agaaatttca cccatctcca
     1441 cagaggaaac tgtgagtgcc agcagtcaac aacacgtctg atcttcaaca aaaggacaat
     1501 gtaattggct caagagtaga tctatttcct aatagatcct ggttattgct ctcctgtacc
     1561 tgtcccacta gcagtatctg agccatgacc ctgcccaaga ctctctttgg ctgatcaata
     1621 ggctgtcaaa gtgcctagca ggcacacaga gcgggccatc tgagatttcg ccctctcatt
     1681 gtccatcttc accgttttaa gtgcatttat caggttacac gcctgctctc ctcactactt
     1741 aatgcatggc tgagctgctg tcctaaaaaa cctaatggcc atcagcggac ctttgtcctg
     1801 gtttgtcaaa ttactctggt tggtttttca tcagatcgtt atgc
//
LOCUS       KCVB01000062            1192 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2721pri ultra-conserved element locus
            uce-1039, sequence.
ACCESSION   KCVB01000062
VERSION     KCVB01000062.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2721pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1192
                     /note="ultra-conserved element locus uce-1039"
BASE COUNT          357 a          203 c          285 g          347 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgattgca ctgtcagggt ggcgggcgca gattggggtc
       61 agactcattt acaaaactta gcatcacggc tcgggggttg aggggatgcg ggggagaaag
      121 ggttcctccg ctgattatgg cttgtgtaat tatgtgtgtg tgtttgtatg tgtgtgtgtg
      181 tgtgtgtgtg cgtgcgtgtg tatctgcgtg tgtaattacg gcgaggagtg atgcatgcct
      241 ggcctgtccc tccctctcgg cttagaacag cgtgtcagag agaatatgca ggatttaatt
      301 atagacgaat taaaattggt aatgaaattg ggcatgaaca aactacaaat agcagatgaa
      361 aagggaggcc tgtccctggg ggggtgagac gaagatagcg gtgtgcctgc ctgcttgcct
      421 gtctgagtgc ctgtggatgt gtgtgtcttt ctgtgtgtgt gctgatgatg actgattaga
      481 aaaattctga gatgaaataa ggctaccaga gaagcactac cttgcattag cactgtacat
      541 ggagctacag tacgcttgtg tgtgtataaa aagtgatggg taaaaatagg actattatcc
      601 tcaggtcaca ctacttaaaa ggtaacttaa caccaagttg gaaagcattg tttgtactga
      661 acacgcaccg agtgagtgtt cttacctcca gctagggaag aacttcaacc acagggacga
      721 atataacaaa ttcagctggt gttgagtttc tctttacaca tttcctgctc atggaagttg
      781 tggaatatca ttacatttaa agtagttgtg acaattcaag ccagaaaatg aatgaaattt
      841 acgaaattca tcagaaactt aagtgagtaa aatgtgtcac tttacaataa aaggtcagaa
      901 tgcatttcac aggtattcca tttattcctt gcatgttgat ttcaggtgtt tttctccaca
      961 acaagccaga ctgttcagtc cttttaaaag aaaatgaaga agaaacaaat tttaaggcca
     1021 aaattcacaa aattcaagct cctgtataga aaatgtggca agaaaatcgc tataaaaaaa
     1081 aataaaaaca ttaaacctaa atcaaattaa atacttaaca ataaactaga ataaataacc
     1141 catgcttgct gactgatctt cagtcatgtt aaagctctcc ctcttttctc tc
//
LOCUS       KCVB01000063            1599 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2751pri ultra-conserved element locus
            uce-726, sequence.
ACCESSION   KCVB01000063
VERSION     KCVB01000063.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2751pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1599
                     /note="ultra-conserved element locus uce-726"
BASE COUNT          466 a          274 c          333 g          526 t
ORIGIN      
        1 ttgctttcca tgttattagc tgggctgctt ggtgaggagc caatctgatc aacttatttt
       61 actatataat gctccagctc ccgctgaaag cagtaaagaa aggattgtga ttgaggtcat
      121 ccttgcatcc atcacggtgc tacgtatatt accaaggcct ttttttcagt gtttgggtta
      181 gtgtttggta caacagccaa aggacctttg ttcgttgtca gggtttagac gttactgaat
      241 tggatcacaa cattatagcg aaggcctgcg ctctgtcttc acctgcagag aaaaatgcta
      301 atgatacaat gttcctgcta tcaaacactc atcaggtatc ttgttagcaa aattcacata
      361 gtgccaaagt attgccatct attcaaattc ctgttttact ctctttattg tagttagttg
      421 ttcactaata aaacacagta ataatgtttt tttttccttc tgcagcattg tattggagat
      481 aacttaaatt agctcaaact taagataggg attgaggaat gagcaaaaaa tataaagtct
      541 gcataatatt tttgaatata aattgaggca gtgatggcac agggaaaatg ctatgtgggg
      601 attattcaca gttatataat gtgaggaaga aaaccaggcg cttctcattt tggtcactta
      661 tccctcattg taaaggtctg tatatttcat tgctggtgca gcagcttagg ggatacattt
      721 caaagcagca aataaaaatt ggctaaactt ccaaaattag cacttcaaag tcaaacatga
      781 tttgcagcga gggaaggggt ggaagcagtg tgactgtgaa agtgcatttg taaaagcaaa
      841 ctgtgtgtgt gtatatgtgt gtgtgtgtgt gtctgtgcgc gtgtgtggtg gagctgatgt
      901 acagagttca gcattaggcc ttagaaccaa tttctaggca aattggtttc atttaagaat
      961 gagatcacag agtaagcaga cctctttcgg ggcaagttca tgcatctgta gccatttatt
     1021 tcagttttat atgcattgct tggcacagac tgtttgtagg tagaatatgc ataatcttag
     1081 ccatgggcac catgctatag ttaatgtgtt cacatttacc tgttattacc atgaagttta
     1141 atagtttctt ccatttcatt gcattctgtc aaaaagagct tcaacagtaa tttagtattt
     1201 tgttttccat attccttcaa gcactgttgt ggaatcagtg ctaatcaaag ccacatgcaa
     1261 ctaattgccc tctccagtct cttgcaaata gcacaggcag gcccattagg catcaatgga
     1321 acatatgttg gagtttttga gtgggtgtga aatttagttt attttcaagg ggaataatag
     1381 tcacttgacc acagttagca tctctgctca tattttcatt tattagagat gactgtattc
     1441 accatgtttt aaaacatata ctgtagcttt gtctctgagg ctgtgaggtc acagctgtct
     1501 atttgaagga caccataatg gctcatgagt agggaataga ttattcattc aaatggttac
     1561 ataataaata ttatgcatac agaaacaaag atacatcaa
//
LOCUS       KCVB01000064            1749 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2847pri ultra-conserved element locus
            uce-32, sequence.
ACCESSION   KCVB01000064
VERSION     KCVB01000064.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2847pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1749
                     /note="ultra-conserved element locus uce-32"
BASE COUNT          558 a          298 c          354 g          539 t
ORIGIN      
        1 aaatctcaag gaaacaaaaa caatttggaa aacatgatgg aaatatgcta tttctatttt
       61 attgattgtc tacaaggatt gtttttctct acagtggagc ttccataaat gcttcatcta
      121 catcataatt acttcacaaa ggctgtttct gttgcacttt gtccattttg cttttatgat
      181 acaccaaaat ttccacttta atcaactgtg aaaactttgt tcaatatttt gacttctttt
      241 ttcaaatttc cagcattttc actttgtttt gttttgcaaa aatttaaaat gtgcattaac
      301 aatgctggat ggaaaaatac tttctgactg atgtaaagca acatgttttc tacagatggc
      361 cagattattt cctttacctt tatcctataa aaaggctgtt ttctacatag ggtgtaaagg
      421 ggggcaaagc aatggagctt aaaggctgca tgattcacat taggtacaag ggggaacaaa
      481 cacacagcta gttcacagaa acaacctgct caaaatttat cattcgacct agtcggccta
      541 acaatcgggc gttaaagaga acaaaagccc attccaaata aattcccact gtatttgtgc
      601 tgggatttga tttactggcc tgtggggagg caccatttgg tagcacaaga agaggcgggg
      661 agaggtaggt ggtgtctgag aggagagtgt gtgtgtgtgt gtgtacgtat gtgtgtgtgt
      721 gtgtgtgtgt ttgtgtgcat gtgtgtgagg gggctgggca ccaggggatg gtgggtaggg
      781 gggtgctcat gtgccagaag tgtttattgt gtcatggtga tttattgagt gagcttcatt
      841 tcactgaatg ttctcagata tttatttccc taaaggcccc acaaagatat gaacaggcct
      901 gcatccacat atgtcatcat tgcccatttt acagcgtcag gagatttctc tcgaactaag
      961 agggggaaga aaaagaaaga gggagagaaa cagctagtgg cgatcagggc acctctccat
     1021 caaagccctg taaacaatgg tacctcagca acacggaaat ggcattttgg aatatggctg
     1081 atgacttttc agcgttcatc cctccgcctc tcccctctct ttcctgccaa atacatactt
     1141 acagagcata cataatttaa atcaagactt tgaaggtcca ctcagcaaga ctggtgcaat
     1201 gttaaagaac tcctttaagc tttaagtttt cattgcgtta cagtttctcg ctccttataa
     1261 acagaactcc tcgtttctca aacaggagaa gacagcttta atatctgcaa ggaatcaagg
     1321 agaaaaccat tgaaaacaga aaactattaa ataatagatt gagaagtcag tggttaagat
     1381 tttaggactg tacagaaaca ttaacaaata gtaatcactt taattctact aaattggtaa
     1441 ggattggacc atttaaaaaa atttttgttc aatatcagta aatgaggata ttataaatac
     1501 ttttccaaaa atgatgtaag actagaaaaa aatatattat gtttttcttg atatttataa
     1561 tatttttttt caacagatga ggacaatttt aaatactgca attcaaaaac ttttcactgc
     1621 ataattcaaa tacataatgc agatctaaaa aaatgaacac attttccaag cagatgtgac
     1681 tgtgagattg atttcaagca caagacaaag acggaattag taaatattag gtttggcggt
     1741 agagtgagc
//
LOCUS       KCVB01000065            1277 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2881pri ultra-conserved element locus
            uce-77, sequence.
ACCESSION   KCVB01000065
VERSION     KCVB01000065.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2881pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1277
                     /note="ultra-conserved element locus uce-77"
BASE COUNT          399 a          266 c          341 g          271 t
ORIGIN      
        1 aaagataggg agaaaaaaaa aagggggaaa agagtatcga ttatccttca gagctccacg
       61 tgggccactt tcatcgatta ggaacgagaa agcagcaaag caccagccat gccccccccc
      121 ccccatctct ccagggtcct ttgtccatga agagagcgat ataatggccc tttgagaggg
      181 agtgaaggag gggagcagaa gacgcaggag agaggcaagg gacaagatgg agcaggaggg
      241 ggacagagaa agatgaggag aaggaagagg ggtggctgtg gcatctagag caggatagga
      301 aagttcaggc cttgcggcat gggggaagtg tatggagtgg tggtggaagg gggagggaag
      361 ggggagagag gtaaagaaag acaccaaggc gagaagaaaa agtctcaatg acaaaaggtc
      421 ggggcagctg ggcttagggc gacagggacc aaagggagca gcgggataat ctgctaaagc
      481 ccagaaagga gagattcagc atgcgcgcca ccctcgtagg gcctaagagc aaatttgcac
      541 ataaataagg agccaacttt gctttgaacg ggaggtcgag ggagaaggca tcagaaaggg
      601 agagagagtc agagatacag ggcgaagtgt tcgagccaca gctgcctgct ggctggtcgc
      661 aaccggacct agacatagtt cagcatttca agctctgcaa tgaagctttc tcatgtttga
      721 aaaaagtaag cagagatgtt tatcatttat tcccccctcc accttagttt ttcttctcct
      781 tagctcctgg ccctgagctt tctaaggttg gccctcaaag acttcactgt cttgtgtgtc
      841 gacatccctg gactcagggg acaagtgtgt gtgtgtttga atgctgcagg ctttgttgtg
      901 actgcactgc acctctaaga tgccaagcta ttaacggcca catactagca caatgcagaa
      961 acagtgatgc acactgacac gcatgaagaa cagaataaat gcaaaaggaa aaaattagta
     1021 gccatactaa ctcaatcaaa tacagaatgt ttgcaaacac ttacaatcag tcacacatac
     1081 agccaatatg tagtaaattt tagtagttga cttacattga gtagctcaaa atcaatatct
     1141 cagttgttgt tcaatacata aaaatccctt tcaaccacct tcccttcatc tacatcttcc
     1201 cttcatgagt caccatggat tttataggat caaagacgtc acctgagttc tcctgataag
     1261 tcaacaccat gtaaagt
//
LOCUS       KCVB01000066            1321 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2921pri ultra-conserved element locus
            uce-830, sequence.
ACCESSION   KCVB01000066
VERSION     KCVB01000066.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2921pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1321
                     /note="ultra-conserved element locus uce-830"
BASE COUNT          373 a          269 c          232 g          447 t
ORIGIN      
        1 gtttgaaggc ttcaagattt ctctgtcaca ttaacttgct gaagtgctca tcaaaagatg
       61 ttaatgtgtc tgttcatttc tttctaaggt cttcattttg cttttttttt acgatttaag
      121 ctgcattttt tggaagtggc tgcgtttaca agctgtaata caagtctctt gaaggcctaa
      181 taaatatgaa actgaatgag attcctttct ctctgtttcg ctttagctct ctgctttgat
      241 cataccatcc catgacatat ccatcgtcag cttttcaact cctcctggag tgggccaagc
      301 ctaattggtg ctatgaggaa taaatcaaag caggaaacgg gacttgttcc aaacacaact
      361 cataatgcac taaaaacctc taatgtttgc tgcaactgtc cagtgagttt acatgtcacc
      421 tcatgtaaca tttttccaat actacctcag ctgttgtatg tctgttaaag agtaactgtg
      481 gtttgacatt acacagccat cctgtgaggt acctgtgctg ctctcttggt ccttattcta
      541 atcttattta acagtctctg cctgacacgc aattcaatgc aggcccccag attggagagt
      601 ttccaaacaa tcctgagctc acccaagctt ggaattggtt ttctgtggaa catctccctc
      661 ctccctttgg gcactttacc aatttgacca aatttgttcc atcactggtt ctaagtacca
      721 gaagtgtttt ctgcccacac ttggactcgt ttctacaatt tgcacattct ttgcagaaca
      781 ttaacagctt tgtcaggggc tgagctaccg gctgctgtgg tgtgggggcc catgtttcag
      841 ggggacactc gacggaaaat tactgcaaaa cattagcaac ttttcagatc ggttcacatc
      901 ctgtatgctt aaacatacag cgatacttat tttaacctct actgtttagc atctctgagc
      961 agtatgtaaa cagaaatgat ttaaaacaca ataacacact aatgtagtta caagcagatc
     1021 tgtaagtgtt taatgatgta tttggaaaca acatagctct tcatataaag atacttctta
     1081 aatactgttg tataaactgc tgatgaatga ttgatgacac tgtataacat aaatgtaata
     1141 ataaatgatt aatacatgaa gcagttcttg ctgtaagcat tttagctgtt atatgtgtac
     1201 aattcaatca aaggcaggtt taaaactgtt tagaaatgtg tccagaactg attaacacat
     1261 gttattatat aatgattaca actttgttac atcatgttat caatcattct ttatctgttt
     1321 a
//
LOCUS       KCVB01000067            1128 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 2960pri ultra-conserved element locus
            uce-1041, sequence.
ACCESSION   KCVB01000067
VERSION     KCVB01000067.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="2960pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1128
                     /note="ultra-conserved element locus uce-1041"
BASE COUNT          341 a          195 c          233 g          359 t
ORIGIN      
        1 aaaaactaac actttaccac tacagttctg cgaagtctgt gttgcctcat tagtttgcat
       61 cttaattgtt gtaaattttg tacagtttga aaaacgtgtt gatcgtcttg tttcatgagc
      121 gctatttatt attgccatgt gtctttgaaa aagatgtaaa agagagcact aaatttattc
      181 atttatgcct tctgtgtaat ggtatgaacc gtgtacagtc aaatctatat gaggaaaata
      241 aaactgaact gtctttgggt taccctccag tgcatttctc cttaatagag catgcctgca
      301 gtgatgaatg tgagagtttt cagctgaact ttgtatttta atttatcaaa agaaaaatat
      361 ttgttgtagg tgtttttatt attttgcctt atctaaacgc tcttagagcg agttaacccg
      421 caaattcatt tacccattga gagcatttac cacacactta ggcctctatt caattgtgga
      481 gatccattta gtaccaccaa cagacagtgg tcagtcagtt aacagtgatt gatgctgctt
      541 tcctctctcg ctctcggggt gggggtgaca agaaagatac atgaagcgag agacgacgga
      601 gaggaagaaa gacgagagag aaaaaataaa acaagtgggg aaacagatgc actgatcctc
      661 tcagtgctaa agagacacag cagcagattg cagcgctgca ctgtcaaatc tatccttaat
      721 ccctgtgctg ttaactaatt gtagagtgct ttgtgaaaaa taggcaaata tgctttgtgg
      781 tcaagcaaag agggaatatg gcccttatgc aggcagttag cttgtatgtc aatatgtcaa
      841 gctgggaagt agactatgtg cagggtagcc aggattttta accactgagg ttatgagtcc
      901 tatttctctg gggaaatttt ttgaccagca accaaagatg ttttcagtag tttttatctc
      961 ctttattgat gattgaaaag ctgttttaaa gcctctcctg ctccatcatt tctgtaattc
     1021 tggtggagaa aaatacattc tttataatgc ttgcttctaa tttaaaaagt caaattttat
     1081 atgatattta gcgtaaaaaa gacaagaaga gtctacagcc atgctagc
//
LOCUS       KCVB01000068            1517 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3002pri ultra-conserved element locus
            uce-1004, sequence.
ACCESSION   KCVB01000068
VERSION     KCVB01000068.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3002pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1517
                     /note="ultra-conserved element locus uce-1004"
BASE COUNT          419 a          373 c          310 g          415 t
ORIGIN      
        1 agcagagtcc ttacacgcta gcacagctag caaaggattc tggctttgca atttggagct
       61 gaattctctt ctataaaaga cactgaagga aagagcagct ccttctgctt caggcaatgc
      121 agccttttcg tggatacact gcaaaatcta aatgatggac tgtcaactct cttgcagaaa
      181 tgccactgca aagaataatc tctctggcag tggatcatgg ttggctgtat gctacaaatg
      241 ccgtttcttt gtttatgcga gcatgtagcc ttcacacgca cacatgcaga catgagcgta
      301 tgcacatgca cagatacaca tacacacaca catgcatatg ctatacacaa atacagaaca
      361 catacacaaa atgctttaag cctaaatcct ttctttgcaa gcatacagtg aaatatatgg
      421 gattaataac ctgaatagca ttattttttt cacctctagc ctttaaaata atgcatgctt
      481 ggcttcgtgt ctggatgaac tttgcctgcg tacagtattt gcctggttat cggtgtgtac
      541 aagacctgct gtgtgacctg gtttcatgca atctccatct tctaattagg aggctgcaga
      601 aaggctgaaa cgagaggagg agtgattagt tgcctttggg tatttcataa gtggctttca
      661 gtgccacatt tgcattattt gcttggggtc tgaataacaa atcagcctac ctttgcagca
      721 aattgaggct agcagctaga ccatcaatac atgagatata aaatgaaact gaagaaaaga
      781 aataagcaaa acatgctaac acaatatggg ggataaaatg aaatattgaa acacaagacg
      841 caataactgt ttcagacagt ctaaactcca agtctaccta cagcagagat taaaatattc
      901 tttgactctt gtattaagca tattttcctt aatgttcatc ttagatttca ggtctcagta
      961 attgcggctc attctgtgac tagcagacca tgtgagccct acgttggagc cccatgctgt
     1021 atgtgtcctg tagggtcagt cagtgtgctg atctgtagcg gaggtcctgt ccactcattt
     1081 ggccagtctc cccaaccgct gcatgcggcc gccacagcca ggcctggtca gccatcacta
     1141 caccacttgg ccgtttgccc acattaagtc ttagagggca ggaaacaaag caggcactca
     1201 catgtccaca tataaaaaca cacacacaca catcacacac acatgcacac atgatctcag
     1261 agagtgacag tggctcttgt ttacccagcg ctcccaccct ccctcactct cctttcatcc
     1321 tctccctcta gtctgtcatg ttgtgtgtca cttcccacaa atgtcagtgc caccatggga
     1381 cagtgattaa ttttggtgtg cttggcaaaa tgtcttcaag atgtttgttt gggttgtctt
     1441 ttccaacccc cttgcccacc cccgctcccc agcgtgtctt agagtcgtgg gtcgagagtg
     1501 gcaaggcccc cccgcac
//
LOCUS       KCVB01000069            1764 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3039pri ultra-conserved element locus
            uce-337, sequence.
ACCESSION   KCVB01000069
VERSION     KCVB01000069.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3039pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1764
                     /note="ultra-conserved element locus uce-337"
BASE COUNT          534 a          321 c          441 g          468 t
ORIGIN      
        1 agaccacacc tgttgaccct tgaatgccca ctcttgtgta gtgacaaaag agagagggtg
       61 ctgtaaagta gccgcaagaa gcccctattt gatgttgacc ctgccctgac ccctttgttg
      121 gggccctaac catttcacag gaagatccaa acgcttaaaa gcacaatagg ggacgagagt
      181 gccgccactg acttgctata aagccccaat aaaacggggt gacctgaagg ctaattgaga
      241 gcttgtggtt gggaagaaat taaaggcatc tggtgtatca ccaaagattg ggaaatgaaa
      301 ctcctcttta attagagcct gcattaaagg cacagagaaa gtatgcattt cactgctgtc
      361 agtacaggtg tcgcatcagg atacatggga acagacagag gactttaatt atagtagttt
      421 gaaggaatgg tttgtaaaaa aaaatgtctg tctttctttc tctctttttt cttccttcct
      481 ttctttcctt ctttctttct ttctttcttt ctggtactac tgtactgcct aagtctttga
      541 tggtgaatca ttagttgcat cagtcaaaca gtaggcctgt tttctccctt tttgtcgctt
      601 tttcatgaga acttttggat ttgcatcata tcgcagcctg cagtatatgt ttccacaatg
      661 cgaacaataa attctgtaca gcccatttca tcgctcttcc ctctgctcaa atcctacact
      721 tgtgccgaaa tagcacagaa aaacagagga gagggtggct aattgcaaat aacccaatat
      781 ttcggagttc aaagcctgtg gtgggtttga gtgataattt tggctgaagc atcaccataa
      841 acactaataa aattaagtgc gtcggaggga ggagggggcg tggccaggcg tccctttgaa
      901 cagaccctgt gatcagttcc ccctctcaat tagcgtattg tccacttgtc ctttcagttg
      961 ggagggaggg ggcagtctcc cccctagctt ccctttgaag tgattatccc tttatctcaa
     1021 gcctgagcga ggaatatctt gggggacaat ggcaaattaa agcccatatg gagggaagcg
     1081 tgaatgcctg tttgtaggct cagagagaaa tagaaaaaaa aagtagcaat agagaaataa
     1141 agagagtggg gagagatgag agaggaaaag aggggcaaat agcatcattc tgtttgcatg
     1201 agctgaaggg tgagttgtag atgtcaacac tcatccaaaa aagtaattta gggctcgtgc
     1261 ctgagttgac acttgtagca gagtgacttt acaagagcta aaggaaagaa taagaggagg
     1321 gggggtatct gtcatgacag atggagaaag aaggagaggg aggaatggag agatagaagg
     1381 atgagcatga agaagagaga ggagtgcagt tgtcagcaga atcagatggc ggtcaggcag
     1441 acaggcttga agaagataca attaaagtgt ggagttaaga cgggctttga agttggacat
     1501 ggtgcataac acccttaaag tttgagaagc cagctattca tctgcttgtt atggcttgtt
     1561 aggaccattc cttttaaaag gcccaatttg agaaaataaa ctttaatttc aactttgaga
     1621 aaagtttaca caaatcaaga ctgacaacaa tcaggtaagg atttcatgaa ggttaaaaaa
     1681 gaaaaccaaa aaatttaatg gtcatcatct gaaacttgag taagagactt actgaggaag
     1741 gtgtgggggt gtgtgtgagc actg
//
LOCUS       KCVB01000070            1577 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3080pri ultra-conserved element locus
            uce-372, sequence.
ACCESSION   KCVB01000070
VERSION     KCVB01000070.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3080pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1577
                     /note="ultra-conserved element locus uce-372"
BASE COUNT          461 a          334 c          311 g          471 t
ORIGIN      
        1 tggtatttgc actctgccat gtctgtgtga ctgaagtaaa gtgtacaggc agcattcagg
       61 atgtcatttt tacttaattt gctctccttt acattcaatc attcctgcat catcagatgc
      121 cctgtcagtg ttgaggcatc atagaaaaaa gataaaaatg aaaattcttc tgtgcttttg
      181 agaatcttca cgctgagaag tgaggcacct tgaactcctt taattttcag ccagataaac
      241 tgctcccaca gaatgtcatt atgttttaag tagacacaaa actcattcac cccagtcata
      301 cagactccgt tagcacacac aagtgggttt cgactcctct ctttcatgtc ggcctccagc
      361 agcccacatc cttccatgtg ttcccctgtc cttcactttt cagcatcctc ccttcgatgg
      421 tgcttttgac atgactgtgt cctgaacaga aacagatcat taaaggggaa aaaaattccc
      481 ctggattgtc gtgtttgtta gttttcatcc tgggcagcag ttcagataca gcaaaggctt
      541 ggagccaaag tcagctctga caattcacag caaaacacag cagaaagatt taaaaaaaaa
      601 aaaaaaaaaa aaaaagtgct gaaccaaatc tgcatttatg agctcaatac gtgtactgta
      661 aaacacaact gcagtgcttt gggtaaaggg tggatgctgc aagttgtttg cgtgtgcagg
      721 cgtaaacaga cgcacgcgca tgtgtgagtg tttgtatgcc tgtgtgttgt gtgacagcag
      781 acctcgctga ggaggactag caatcactcc attcagcatc tgctgccaca cagctcatat
      841 tgtcgttccc cagtttaaag gaaccttgag cagcttcaaa gatcttttca aagccttaat
      901 cctaataggt cgcggtttgt tctgtcacat caaaaggggg agagaggagg tagggggaat
      961 aatgcatcgg cgctggcaga gattcttaag ggcggaagat tggcagagga tttgcagtgc
     1021 ggggaggact gaaatctgaa atatcggaca cagaatgcct gctgtaatag atatgaaata
     1081 ggtcttctca aaagcatgaa aggctgacat ttccagaaag tttgtcatga gaaggccctt
     1141 ttattccctt ccgtctatct gcgtttgtac tgccaccttg tccatagtgg aatttcgcaa
     1201 gtgtcactct aacaggttat agaataagat tggacatttt agattttttt tttacccaac
     1261 ctcttccctt tttattgaat gtttagaatt tccaagagag accaattgag ttctgccttc
     1321 tgcatggcac tcaacttcta taaaaactct atattcagca cttgtttcct gaccttgtgt
     1381 tatagccagc tgaatgaaat aaaaatcgca gccttatacc ttagcctctc atatacatat
     1441 gatatatatg actatataat ctatgtattt ctctctctct ctcacacaca catacataca
     1501 cacacatata tatttttata gatagatata taaaaaatag atagatttag attcatagat
     1561 atagtacatt gtctgtc
//
LOCUS       KCVB01000071            1757 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3120pri ultra-conserved element locus
            uce-1340, sequence.
ACCESSION   KCVB01000071
VERSION     KCVB01000071.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3120pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1757
                     /note="ultra-conserved element locus uce-1340"
BASE COUNT          477 a          385 c          458 g          437 t
ORIGIN      
        1 tgaagcaaag gatgctcaga tgaggagaaa tgagaggact cactccctct ctttctcacc
       61 tctctctcca tctctgcctc tctcctcctc tctctgtctc tgtatcagcc cccccccccg
      121 aaaccaacca acccaccctc ccacccccaa cccatctctc tgtctgtctg tgcctctctt
      181 tgatctcagc ctctgtccca cactgcatat taagatgccc gggcccctgt ttggtattct
      241 gaggcgggca ccgggatgcc agtctccata tcctctcctc gcttctctct gaaagagagc
      301 aaaaacccct ctgtctgtct caagatgctt atcccgctaa atgggtctga aatagctgga
      361 gaacagggcg ggggagcgat ggtggcggca gggtagagaa ggagagagat gcatggtggg
      421 ggagagggcg gtaaggggaa gtattagaca aaccacagac gggtggcggg ggaggagagg
      481 gaagagtagg gatgggggta agggaagaga agcagagtga tggggctgat tttagctgag
      541 aggataatta aacgctatta tcacaatggg ccagtaatac agctagcttg tacatagaga
      601 agccagccct cccatgtttg cctcactaaa aggagggagg aaggaatcag ggaggaggga
      661 ggcagaggag agggaagcac agattaatag gcggaggaag agcaagagaa acagagggag
      721 aaaaacaggg aggcacggag gggggagaga gggagagaaa cggtaaaata ggcaaacaga
      781 attagaatgg gagagtgagt gggagagttt agcggtaggg tgattgctca gtgtttggga
      841 ttagcatgac tctctctcac ccactctctc tccctctccc tctctcgcgc tttccgtata
      901 tctccctctt tctttctctc cctctccttc tataagccct ggggatggca cagctttagt
      961 taaactgctc tattaaacgg cgcctgctga gaaaatcaaa ttataacttc ctttaatcag
     1021 cgagtatcag tggcccacac aggagcaacc ggctctgaca gcagagagag agagagagag
     1081 agagagaaag agagaaagag agagagtaag agagaaggag actgtgactg gagacctgag
     1141 ggaggccgat atgaacctgt ctcccgtctg ctttggttcc catggcaaca agatagagtg
     1201 agggggaata tgggttgtgg aatgtctgca tctatcgttt cctctcggta agcattcata
     1261 cacagagata ggagagagga agagaataca ctcagtgagt cagggtgttc ttctctttgg
     1321 gtggcgattg aaggcgggtg ggtacgcgca ctcacaacta ttgtcatcta tgtgcctgta
     1381 tctgctggcc cctgtgacct ggaccatggt ggaaagtaac tgagtacatt tactcaaata
     1441 ctacacttaa gtactatttt tgacgtccct gtactgcatt tgaatatatt cattttctgc
     1501 tttgtctgct ccactaaaat tctttttatg cagccatatt taacaatagc ttaagtttaa
     1561 agttacctct cagattaagg tgtaatatta aaaacatata ataatattat aaaacataat
     1621 gcatttttat gaattaagcc agtagttacc aacctttttg gcttgtcacc tcttgcaaaa
     1681 gacaccgtat ttacttgggg ccccttgcca cgatttcaac gatttctcct agaactttgg
     1741 tttgtatatt attagat
//
LOCUS       KCVB01000072            1234 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3164pri ultra-conserved element locus
            uce-542, sequence.
ACCESSION   KCVB01000072
VERSION     KCVB01000072.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3164pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1234
                     /note="ultra-conserved element locus uce-542"
BASE COUNT          349 a          251 c          342 g          292 t
ORIGIN      
        1 ttgctcagga gccttaaggt gacaatggct ggaaaggata tttttgaagc ggtttccaat
       61 gcgatcaatg atatgagact gccctgggac aacctttgtg gaattacaac ggatggagct
      121 ccagcaatga ccagccaaca gaatgggatg actggtgaaa tgcactgtat tatcctccca
      181 tgacatatta agccctgcga ctccttatat tttttctgta tatgacccac aatcaaaaaa
      241 gtttggccaa cccggcatac ctagttttac agttttacaa aaagcagtga gaacaggaga
      301 cagagaggtg gagagagaga gagaaggcaa tgaataaaaa gtggcagcag cagtagaaga
      361 agcagtgtat ctaacatgtg atgagcaaag tccggaggag ctgcagaagt tttgtctctc
      421 cctcagtagc ccattccctg gtccctgggt gcgccttcct accccccaga gggcctgttg
      481 tttagggctg cagagccacc tcggccttga tgaatttctc cccgtcaaca aattaagagc
      541 tgctattgtt gctgtgacaa caaggcatcc cggccgccat ggggagcacc atggagaccg
      601 gtgacagaac tccagaattc tcctaatcat ccccagctgt caggaagcac ctggcctgac
      661 acctctgctt aaaacctgtc tcccaggcat aagataatta caagtgccag cagagagaga
      721 gggagaggga gagagggcag cgagaaggag agctggtgag agaggagaga aagagagaat
      781 tctttcttat aagtggctgt gtgaggaacg gagtgcagga gcttttttag acaagttgcc
      841 attgaagtaa aggaagtgtt caaaggcaga agtggagttg tatgtgggga ggtgtctggg
      901 gtgtctcccc tcttccctac agagaaggcc tgggaggggg tccctagata gcgctgtggg
      961 ggacaggtga gggacggagc tgtcaccggg cccacattca cacacattta gaagacagag
     1021 actgtttgac tggtccagta caaatgctcc aaggattgca ccacattttg gggggtaaca
     1081 gtttgtggag tatttttttt ttctttttca ttattatttt agaaaagtta cactaaagtt
     1141 ctgaaagctc tggtctgctg tatgtatagt tgggagggta aatagggtaa tggctcggag
     1201 aaaagagtgt ccagagctta caaaacaaaa aacc
//
LOCUS       KCVB01000073             960 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3196pri ultra-conserved element locus
            uce-995, sequence.
ACCESSION   KCVB01000073
VERSION     KCVB01000073.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 960)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3196pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>960
                     /note="ultra-conserved element locus uce-995"
BASE COUNT          317 a          159 c          197 g          287 t
ORIGIN      
        1 agcatttgca gcaaagtttg tggtttatcc agggacactg tttccagcaa caaaaaaaaa
       61 aaaaaaaaaa aaaaagtcaa aaaatgtttc atgctctgat gtcattagga aggcgaaagc
      121 agagctgtaa tcctatttta atcgcagtgg atttattaaa caggagcagc aggtttaaat
      181 ctcaccagat tacacagaag gcccaaggag atgtggagag ggggtcggag aggcttgtgt
      241 aatcaagtgc acttaaggtg atatggttca tctttgacta ggcaggactg attgagaggg
      301 aggggcttca gggggctcaa tgaaatgtgg gtacagtcag atggctttac agtaaataaa
      361 ctttctttaa aatgatctac ccatcagaat tttgttcata ttttgttggc tttgatctaa
      421 attaaatatt ctattgagaa gtcttttaga ataattactt ctgacaagat taaaacagat
      481 tgcctgcaga tgttactaat tatttggacg tctgcgtgtg cgcctccgtg tcccttggag
      541 ttggcgcaaa tctccttggc accctttgta aaagacctag attcttgtgg agaacgggtt
      601 aatcccaggt cacatcacat ctggacttaa tcaaatcaat cccataacag gacttgtaca
      661 acaagcttgg acctctagag gtcagcagta ataaaaagtt tcaaatcaat gaaagtgtca
      721 gtatacatgt ttgtattaat gcttgtcgca cattcacaca cgtatattgg aaaaaaatgg
      781 ccaaatattt ctgtacctca acataatgaa aaagctgaat gttgttcctg actgggtaag
      841 ttcatacaag tacaccaatt gagagaagca acatacatat ttattgtagt attataaaat
      901 ttcagacatt aaatttatat tttatcagtt gtactataaa gcattatttc tggactgatt
//
LOCUS       KCVB01000074            1672 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3308pri ultra-conserved element locus
            uce-292, sequence.
ACCESSION   KCVB01000074
VERSION     KCVB01000074.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3308pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1672
                     /note="ultra-conserved element locus uce-292"
BASE COUNT          439 a          417 c          363 g          453 t
ORIGIN      
        1 atcgactaat tgctgcagct ctaatttcag cactagataa tatacacagc tctaaataac
       61 gggtaatttg accaagctga tgaaaataga ctcatttcaa gtgtgattca ttgccctgtt
      121 ttttttaaga acagtacagt cacctcatta aaagcatact ctgtgttgcg tgtactccct
      181 ctccttttta ctcctgtcca tcactgacat gttgaaataa ggttgctgat agagtgacct
      241 tattttaagg catttttcaa cccgtctgat gaaagatgag gggaacgcga agataggaca
      301 gaatcatccg tttctgcatc tgtggaatgt gtgacccagt gttacccttt tggatctgtt
      361 cccatccaca caggaagtcg aacattaaag caatgccatt tcttcctttt ctcttccctt
      421 tccctcctct ctccctgcat tcctttgact gactgcggca catttcagga cagcttcatt
      481 cagcattcct ttacatgccg atttgtttgc cctgcccttt tatttttttc aagtctccct
      541 ctttttaggt ccggcttgat catgcgagag ccaaacacca agacaacttc atctcatcca
      601 attttcaccc ctttaattca gtttaaatgg catggccgca agggccagaa cgggaattaa
      661 taagagccaa taaagctcgc cactggggca tcgaggccag tctttaagta aacattatga
      721 cttcttgagg ggggattcag gggcagcact tgatgaattg tggcggagct tgggaagggc
      781 taatttctca cttagccgtc ctgatggggc tagctaatag gccttgcttt gcctggctga
      841 ttgtctgctg gctttattat tgtgcattat cgcgctgccc cctggctgtt gttagcgttc
      901 ggccccggag acgccagggc ccgtcattaa tcacatagca gcactgactc actctgctct
      961 aacacttatc ccaatgacac aaaggaatga aggaaaggaa agagagatag aaaggaggag
     1021 cagaggaatg agaggaagag agagtatgtt aggaggaaaa ctaaacagaa gagaagggaa
     1081 agtccaagtg ggcagaaaag cttcacagtg acttcgtaac ctgtagctgc acagctttca
     1141 gattgctctc agtagttgcc tcaaatagca gtactctttg ttagacactg ccttttaagg
     1201 cacagaggat aagaggagtg tattccacag tcccctgggc aaagtgtgaa gaccttctgt
     1261 caaagccata cctgctgcca ctacagtcac acaggaccat ctatctgctt gatccttctc
     1321 tgagcgcacg tgagaggagg aggcagcaag agatgtgtgt gtgtgtgagg ttttcatcag
     1381 tacaggaatt tctcttcctc cactcatccc ctccctgggg tcccctctcg tatttatgtt
     1441 ctgcgcacag cgatcccact tgcatactga gaggcatgta gagcacctgg tgcaccaaac
     1501 tcctatacat accgtcatgc agcgaggctc acacactctc aaacacagct gaaacagtcc
     1561 ccctttctca ctgtgccatg ttctcacaca catacacaga caaacacaca cacatgtgcg
     1621 ttacatagga actgtgctct caaacaacgc agcacacggc gtaacatgta ct
//
LOCUS       KCVB01000075             510 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3341pri ultra-conserved element locus
            uce-643, sequence.
ACCESSION   KCVB01000075
VERSION     KCVB01000075.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 510)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3341pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>510
                     /note="ultra-conserved element locus uce-643"
BASE COUNT          184 a          142 c           98 g           86 t
ORIGIN      
        1 acacacacac acacacacac acacacacac acacacacac acacacacac acacacacac
       61 acacacacac acacacacac acacatacat gcacacatac acaaaaatta tcctgcgcca
      121 gaccaaagag acagatgaca cccagcatga agctaaagcc aaatctgtaa gcatccgctg
      181 cgatccagct ggctagccct agggtacctg tctctgcgaa gtggaatcag cgtcaccccc
      241 gtgagctgtc aaattagctc acagggctgg aaatgctgaa gagatttggc ccctcgttgt
      301 ggaagagcaa aaaagagggg ggaagggaga gtggtattaa aaatgctatt aattcatcct
      361 cctgttggga aaaaatggtt aaacctccaa caacataaaa atcaggagag gtggacttat
      421 tagtgtcaac ctgtggttag tggaggcttt gtaatgaaga ttcacacagg acacacgtat
      481 acacactcac acacacacac acacacacac
//
LOCUS       KCVB01000076            1138 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3381pri ultra-conserved element locus
            uce-1119, sequence.
ACCESSION   KCVB01000076
VERSION     KCVB01000076.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3381pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1138
                     /note="ultra-conserved element locus uce-1119"
BASE COUNT          360 a          227 c          248 g          303 t
ORIGIN      
        1 gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga gagagagagg
       61 aaaaaaaact tgccatccgg ttatggtctg tgagcagaaa attgtaactg caatggtttg
      121 cgacatagaa ccaatgcacc cctgcatgta ttgaagtgag gttagaaggc taaactggct
      181 gtaacattta caaaaacgac taaatgacaa agactgcgag gctgctgcaa tggactgtac
      241 gataaaggta tgtgtttgaa ttgtttttta ccgctgctgc tgccgttact gctgctggtg
      301 aacatgacag gcggccaccc acaccagccc tgagtctgct gagtgagaga gtcaaggcgg
      361 tgatgcgagc tccccactct ttccttcttg tacaaaattt cctcttcctt cccgttcgtg
      421 gttgtatgtc ggctctataa taacacaact aaccactaca tctttacact cagataaaaa
      481 cactaattgg tggccggtta tacgcgtaaa agaggacttc caaacgcccg tgtttcgttt
      541 ttttctaatt gctgcgttta atcgagctaa tcccgcacta tccacggacc cacaaaacaa
      601 caacagagct aacgcgcgga gagtgaaatt gttgaacatt tagagggttg acaaacaatt
      661 ttatttttct ttaaagaaga ataagttaat gaagtgatgc agtacttaat tggtgcgcaa
      721 caggcaggtg tccctgaagg cgccatggat caacttggat acattgagaa ccactttcaa
      781 acgagcaaga ggatctattt ccatcagatg gctggtattt cacacttcat actataactg
      841 ctacacctgt tagtcttctc cagaagaccg cgaagcagta attgtttcaa tgtcttctct
      901 tctaagaaat gcagaaagaa atgccttcat gacatgattt gaaatcgaaa aacacttttg
      961 attaaatgaa cactgaaaac tgaatattgc gcaacaagag aaaaggcaaa caaattgatc
     1021 tgatgacgca ggcaacaaat ttacacgtca acttttttct ttttaaagaa tgtgtgtcta
     1081 ataacctcat cggcctgtag gcatttattt tttcaatttt gcagtaaaac attcatgc
//
LOCUS       KCVB01000077            1183 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3457pri ultra-conserved element locus
            uce-313, sequence.
ACCESSION   KCVB01000077
VERSION     KCVB01000077.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3457pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1183
                     /note="ultra-conserved element locus uce-313"
BASE COUNT          363 a          251 c          250 g          319 t
ORIGIN      
        1 ttggctctca gtaacacact gagtcattac agtctctgct tgatttctgt tcaatattta
       61 tgagtaacaa aacacacctt cggcaggaag caatattagc tacacaatta aactagacca
      121 tacacatcct gctgttagta ctgttcatta tcatttcgtg tacacttact ctaaatacaa
      181 agcttccgtg acaaaaataa ccactgaaaa tatgcacgtt aggttcaact ttagaaagag
      241 ggaaggaaac cagaaagtag cttgttggta aagaatgtgg agagtctcaa gaaaatagaa
      301 aaaaactgct ggtcaaacat aactccctca gagcgatcat tgccctgatt gggtgacaaa
      361 gatgaaaaag gccttttcaa taactcagac taatgttgtg cctttgctca gaccggctgt
      421 tgccctgggt gcgtgtgaaa tgcttggcgt ggacaaagta aaccacggcc ggtgcccatg
      481 ctccattgtc ctcaaagcct cctaaagagg tctcttcaca ggtccagcaa acacactctc
      541 cagactcttg gcatatctct gaagatcctc cttttgtgtg aggaaacaca ttactctctc
      601 aaggagagga acaggggaaa caaggcagca ctagtgaaaa ggcttaagta tctcaccccc
      661 caccccagac cgcctcttct ccttgccaga tgataattgg gactgaaaca cttttttctg
      721 ctccgagctt aattcagtga gtaaattgca acatgggaaa aacatgaatg gggcccaagc
      781 tgtgtggtga ctcagccaca gagacgctaa gagtcgctct ttgtggtcgt ctacaaaagg
      841 gaaatgttct gttaatcagc attgttatat aagtaagtgt gctttccttc aaaactatgt
      901 gattgaatgc tcctcctgaa ggggtgaatg aaaatattga aagtgaggga tgagtattag
      961 cactgcacat attatatgag cataatgttg ggaaagtaaa cttgagtaaa ctcacagtta
     1021 taacatgtgt ctgtgcagtt catcccttcg tttttgttta ggaaaaaaag taaacagaaa
     1081 gttatgagtg tgtagtgcga ctgcatcaat cagtcatttc tcacagcggt ttcatttctt
     1141 gcagttgtca tggaaaacaa cccacaatgc tgacctgacc agc
//
LOCUS       KCVB01000078            1172 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3498pri ultra-conserved element locus
            uce-356, sequence.
ACCESSION   KCVB01000078
VERSION     KCVB01000078.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3498pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1172
                     /note="ultra-conserved element locus uce-356"
BASE COUNT          328 a          237 c          267 g          340 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgcgcgcg actgcgtgtt tgcaagggtg tgtgttttct
       61 gccaccagca cctcacaccc cttagacaga cagcaggcat tcgtgtgcat tgtatgtctc
      121 tcgaatatca atgaaccaca cacacgcaca cagagtctca tactcacaca ccaggacctc
      181 agaggtcaga gagtgagtca atcatctaac tcattgcgac gacacagact gaaaaaaggc
      241 gcaagtgtgc atctgtgtgc atgtgtgtgt gtgtgtgtgc tgggagcagg gggccggcag
      301 ctgtcagggg ctaggagcag caggcactaa tgaagcagat gaatagtgca aaaactcgca
      361 aatacaaaca ggacagttga catttttcat ctgtcaaaag cagaagatgc atgaaaatac
      421 gatggggtct tgtttaaccc tttgcctccc ggtgtctaga gctggctgat ttcctttttt
      481 ctctctgttt ctgtcattct cttttttttt ttttcactta cttagaaatg tatggttttg
      541 ggaagcccac agacgtccac cctacctgtt ctacagtact aaaaggtgta catatatttg
      601 ttacaaggca gtagagagtg ttttcaccct cagccctctc caggttattg aagtgaactt
      661 ttattgcaag tagtcagcat tttttgtgca cacatgggca ctccagacca actcataccc
      721 ttttgaaaga aagccagctg agtctgcctg gcactgccct cttgtttttt gcattttgag
      781 agctctcagt ttgttggttg gaaagtgttt tagtttctga aataaaagtg caagatgtcc
      841 actgaccttt gtggtaaaat tctcatgata aaaccaaagt tttctgacct cactggactg
      901 atgacgtatt tataggctgc agcgaatggc cacatagctt gtggaagctt gctggaagct
      961 taaacaaaca aaaaaagggg gggaaagaca actgaaacat aacagaaact gaaacataat
     1021 ttaaaaaaat agaggactga taatttaaac gggatgtccg gcgtaatctg tattaatatc
     1081 tttcaaaatt acatacatac gtattaacgt gctttttact ggtagaaact gaaccagtgg
     1141 caaatatgcg ataggttatt acatgttatc ag
//
LOCUS       KCVB01000079            1357 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3538pri ultra-conserved element locus
            uce-787, sequence.
ACCESSION   KCVB01000079
VERSION     KCVB01000079.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3538pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1357
                     /note="ultra-conserved element locus uce-787"
BASE COUNT          438 a          290 c          324 g          305 t
ORIGIN      
        1 caaagccagt caaaaaaaaa aaaaaaaaag aaagaaaaaa aaggagaggg tgcaaaagct
       61 cagagtagaa atgtctaact ccatgtcccc ccttcgtctc ccttttctcg ctccctcttt
      121 gacacaccag gaaaaagaca gatataaata acctcaggta cagcactgct atttttctaa
      181 atgaagagca aaataacccc cgcactcgca ctcaccactc ttgttctcaa aactgaaaga
      241 gtgggcctcc attgaaggat ttcaaaaaat aggggcaatt ttaatcatct ctccattcaa
      301 tctcagttgg ataaatcata tctgagagat ttgcataaat acaaaccatc aaagactttt
      361 catcttcaaa gaggttttca agacccaata ggctttgtac tgtaatcatc tgaggtcaca
      421 accctgtggg agcaaggctg ctatggcaac ataaaaacta acgctggggg cttagcataa
      481 tatcccccct ccttcgcaaa cacaggaagg ggtggaggtg tgcggggggg tccgagatgg
      541 gagtgtgaaa agagggtgta gataaaccta tttctgtgac agaggaaact agatagatgt
      601 ggcccgttgt ggggaaacct agctgatacg acacacagag attaccagca caacactgag
      661 ccacgcaaac cctaacaaga gtgtggtgag ccgcgcagcc tgtttggatt ctgccggagg
      721 aaaacaaatg acagctcaat gcggcaccac tggataaaga gcctcctagc ctctctgatt
      781 acatgtttct gtccttctgc tgtattctcc tctgcagcta ttatacactt tgagggtctg
      841 atttacactg cataagagcc actataatgc ttgttcattg ttcctctggc acgtacagca
      901 accattagct gtcaatggct ctcaaaacta ggacacttta agatgttatt cactactgaa
      961 ttggaaaaaa agaaagggaa agaaaaagga agaaataaca cctcttcctg ccattcaaag
     1021 ccacgctaca aacaagccct ctgacagttt tgccacccaa tcatgaaaag agaaggaggg
     1081 agaaatgaag ctagagaaag gaggctatag tgggacaagt ttcaattaat tttcttcaaa
     1141 agggggccag attaaatgtt tccggcctgc tgaagtgttc atttcacaga ggggaagcgg
     1201 agaaagggat gaggggaggg gacaggagga ggagtagagg aggaggagcg ggacagggtg
     1261 cgattctttc ggtcccagtg cacaccaaat agtgtggtgg gcaaagaatg cacacggctc
     1321 cgaatctgtg tgagaattgt cctcagacaa aggaggg
//
LOCUS       KCVB01000080            1481 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3582pri ultra-conserved element locus
            uce-1098, sequence.
ACCESSION   KCVB01000080
VERSION     KCVB01000080.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3582pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1481
                     /note="ultra-conserved element locus uce-1098"
BASE COUNT          452 a          285 c          297 g          447 t
ORIGIN      
        1 ataactaata cccacagccc aaaggaagtg gaggggaggg tgctttatct taggaacaga
       61 taacatttaa accacagagg ggttttaagc agacagcata tagcagcaca ggtcaggtca
      121 cttgttcttg ttacagctgc agagtgtcaa gcaagtgaaa tgaattaagg gtggacaagc
      181 tatgctgtca tgtactattc agttcaatag ggcatctgtt tatgcatagt tttgaatact
      241 taattatttg tgggaagcgt cacatgcctt tacagtatgc acctgataaa caggatctta
      301 gggcattttc atggtagatg tgtgaccaat ttcaaatcaa atcaaggtta cctggtaaag
      361 cctttggccg tatggagata agaacttatg cttacatcaa cacttgtatc tgtaaatctc
      421 ggcatagaaa atctcaaacc ctgtgctgct cattttgtag tcttgtgctg tttcaaaaac
      481 ttctttctag tatttttctg catatatcag cagccggtga ccacatactg taaaaccaaa
      541 tgcgttgctt gttaaaatca tatcaaatca aagcgtaatg attgttcaaa ttcctccttt
      601 ttccctgaaa tacagttaat gaaagtggag aaacacagta gaggaaagga aatggaacag
      661 aggtgagtga aagaatatat taaaccttga attgagagat agaggaaaaa tattcctcag
      721 tctcagcctt tggaatgcgt tattagggaa tgatttcaac aggctcttag tagctctcat
      781 tagatggtgt atcaaaccag aacaaaactc acttctccta actatgtgga ggaagtggat
      841 tggaaaatta aatggggaga ggagagtgag ggagcagtct ctgccacgct cttgtcaccg
      901 tgcccctgct cccactaaca agtccgctca gtcgactcgc tgctgaaagt cattcacatg
      961 gtctttaatt tcacttcact gattggaatt taaagcccca gaatatagca aggaagacat
     1021 taagattatc tattccctaa accgtgtgcc gaacatttgt ttttaaattg tggtacagta
     1081 cagcggaaac cttctctttt gagtttgcag ccctaaaact tcagaaactt tgcaaactga
     1141 gcttccactc gcggctgggt actatataaa tttctgcagt ttttcttaag aagtatatat
     1201 ttatatactg catgctgtag atcaataaaa gtaacatttc attaatcttg acatgctgtt
     1261 cctgcttgat gccagatgct ggggacacaa gctgtcgaaa caatagacat gctcttcagt
     1321 gcttacttta cccttccctg tttttctttg tttgcacttc caaagacagc attacatctg
     1381 tcagcaagat atgatttaca taaacatcaa actattcttc tgagtatctg tcaagggaaa
     1441 ctcttgagac ttgcaaaata ttagcatttc aaggtttgca t
//
LOCUS       KCVB01000081            1020 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3612pri ultra-conserved element locus
            uce-1065, sequence.
ACCESSION   KCVB01000081
VERSION     KCVB01000081.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3612pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1020
                     /note="ultra-conserved element locus uce-1065"
BASE COUNT          290 a          228 c          227 g          275 t
ORIGIN      
        1 tgtcaactaa tgtattgtta aacataggaa aagattgtgg ttgcagtcaa atgtcaattg
       61 caggtctgtg cctgagcata catggggcag aagttcagac tgactttagc ccagtttgga
      121 ttatttcagt caatacagcg aatgcaaacg cagacgtctc agaattgccc tccataaaat
      181 aattaacctg gctcaacttt tgttgctttg gcaagacgct gtattagcca ataaaaaaag
      241 ttcagctgca cattcaattc attcattcaa attggttttc ctggaaaaaa aaatgtttct
      301 aagcagtacc tgaaacaaca cataaccacc aattgtgctg ttttgatgct gtgatgttca
      361 tcgaatgccc taagatcgaa accctgactg tcgactcact acatacaggc atcactcagt
      421 gtggacataa ctgcagggaa actgtgctgg agtattgttt caagacaggt gtgaagaaat
      481 cctgacctct cctttggaaa aaatgcaaaa gcatgcgtgg cattcattca cacacacaaa
      541 cacacacaat tgcacattaa ctaaatcgac aacatccaaa ttgaacagcc ccaatttgca
      601 tctccactcc ctcaactcat aacttttttt tctttttttg ggagggggat gaagttgcca
      661 aacttcttcc ctgttgtgtt tttcccagtg cggtgctggg atagtttgga gagcttgctg
      721 tgttcccgtg gcgacagact gcggcccctc tctgccgcgc tcaagggccc ttgaggcggc
      781 cattatctgg acgcccgcct cactgaggca tatgggtaat tactgagcta atagagtgat
      841 ggagggagag gcagaggcag gagcctaggc caattagatg atgcattagt gctctgtctt
      901 ttgtaagcac ctaatgtgta gggaaaggat ggggtagata gagaaaacgt gtcatttctg
      961 aagggtcagg atccttgagc aaatgcaaac agtctctctc tctcacacac acgcacacac
//
LOCUS       KCVB01000082            1210 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3652pri ultra-conserved element locus
            uce-1020, sequence.
ACCESSION   KCVB01000082
VERSION     KCVB01000082.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3652pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1210
                     /note="ultra-conserved element locus uce-1020"
BASE COUNT          404 a          212 c          223 g          371 t
ORIGIN      
        1 acagccagtg aggtgtaagt ttctgtcaac acactctgct atgaatgtta attcactgat
       61 aaaatgtttc atacgaacac gtctcattac aatgtaggca ccactatgac acgctccggc
      121 ttctttttta gttagaatgt acagtattcg gggcttcatt acaaaaacag atgcacctga
      181 caaaatctac tctttagtaa agacattgat gtgcttttgg aataaacaaa ccgcttgcta
      241 aaaaataatg tcagagttac gtttggattt atggcctcca gctgctgtac aaattaagtt
      301 ctgtgtcatt tacgtagaga aattcaatct ggtgttggtt gcagtttgct gttcagactc
      361 tatgaagata atcactgaga gaagccggct catcatgaaa tattcagcag actgtgttaa
      421 cgccttgaac tgaaagcttt ggcagcgttc ctcctccctg ttcatcactg tgacacagag
      481 ctcctgatgc acacaccgat atgaatgtag ccatccttgt agagtctgtt gatgatgagg
      541 gtgaggatga ggccaatgtt gcgggagttg taataagtta aatagatgat ccatccacag
      601 gacagaatgg tggctgaaag acaacagcac aacatggtca ggtttgttaa tataaggaat
      661 aatacgagac aattagtaaa acaacatttt ttatgttttt tgatgcttgg agtagtttgt
      721 ttatattaat ttattgtaat tctacatcac aggattgtgt tatgaaatta ttttgtagtc
      781 atttcatgct acacacacat aaaccatacc tatattaaaa caagaataga ataaaataaa
      841 aataaaatac aacaaaaatt acaattactc agatatattt acatataaag caatatatgt
      901 aaacacccag acagacattt gaatcagtga tctttgtgaa cctaacagtg aggcaggcct
      961 ttactttcaa aaccaccaat gctgattttg ccctgttaac aaactcttta attatacatg
     1021 tgaattacca cgattggtcc tttgaattca atgtgtcaga ggctgatgtg acagcacaga
     1081 gaaatggctg cagcattaca gtggaagtca ttccagcaat agaattagga atctattact
     1141 gaatcataaa ttctacttga aaatgtgttt tttaaaggaa tatagcaatc cagatctaat
     1201 taataattac
//
LOCUS       KCVB01000083            1037 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3764pri ultra-conserved element locus
            uce-486, sequence.
ACCESSION   KCVB01000083
VERSION     KCVB01000083.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3764pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1037
                     /note="ultra-conserved element locus uce-486"
BASE COUNT          314 a          193 c          199 g          331 t
ORIGIN      
        1 gcatgtaatg ttaatattta gaatgtgcag ccgtataata attatccagt cagatttaaa
       61 gtcacagttt aggtcaaatt tacagtatta taatgtttta gcataaatat tgtcaattta
      121 gataatggat caaaggctaa acagtctatt tcactattta attgcatctt ctttaactat
      181 gtggtagtaa cttaaaagtt atggtgcttg agtcattgtt gatgaacaac cttaactgag
      241 ggagcaaatg tctctccagc acatgaccat tttccctgtc aatctttagt caatgcgctg
      301 ccatcaacaa gcacatcccc taattaacta aaattgtttt tcatcaatac ctataactga
      361 ccagcacagg aattgatggg agcgagggtt gacttgacta aaaatacctg aaggggacaa
      421 ttcattgaca ggtaaagaat ctatcattcc tcctgtcaat agaataaagt gtgtgtgatt
      481 ctggaaagtg tgttttacaa tctgcagccc ctttttgggc aatatagcct caatggatca
      541 attttagccc cacctaaatc ttggaagtaa atggtttgca tttagaaagt cattacgctg
      601 tcaattgaac atctcctcct ccacttcgtg tggggatgtc tataatgaag tttgttgtca
      661 ttatagtagt tgaatatact aatcttgtga tgtttccttt ccctatcatg tcgtgttagg
      721 gttgatgact ctcagcagac aatctcctcc attcaggagc ggtgccacca agtctgtcct
      781 tgcttatcat taacaatctg caaataaaca ggacagcata cattacatca cttgcagctc
      841 catgatcaat tccaggaatg tctttgaagc tttgcttaat gaacctacac agtatggctg
      901 ttgtgtgtag tcagggcaat gaggcttcat aacccatgac cttaatgcag ttttcctcat
      961 tgataatatt gggaggttag caaagggggc gaatggtgat gagactattc aataaaaaaa
     1021 aaaaacagtg gttgcag
//
LOCUS       KCVB01000084            1382 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3809pri ultra-conserved element locus
            uce-814, sequence.
ACCESSION   KCVB01000084
VERSION     KCVB01000084.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3809pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1382
                     /note="ultra-conserved element locus uce-814"
BASE COUNT          302 a          324 c          286 g          470 t
ORIGIN      
        1 tttattatta tagtagtcag tcatcagttt atctatttta tttcattttc agtctctttt
       61 cagtttttct gttcttcttt gaagggactg gtttgttctc tgtacctctc gtgtttttct
      121 ccacctggtt tcactcccca gagatttttg attgtatgcc ttgaaatctt tttattcaca
      181 gctgtgcctc tcagtgcttt catagtaact tgctggtggt gtctgtctcc agagaacctt
      241 cactggagca ttcaaaaccc tccaaagact tgttttgcag gtgggctttt agatcacttc
      301 aagctttaga ttttaggaag tggcagttga atctgtcatt gcgttttgaa tcaaatgaca
      361 caaccctatt tcaatatcac agccatgatg tctcctatca gacattcttc tcattgaagt
      421 ctgtctaatg atggagccat acagcgacag actttaaagc attggagaag agccatcgca
      481 actttacaca tgccttctct gccgtggctc agcagttggc gtttcacatt caagctgcgt
      541 cgcatccttg ccggcagctg tgtcaggatg atgcctgagg tgcctcctag gaggaccgtg
      601 ccctcgttac tcctcaactt gactttgggt ttcacaaagc gagccctttg tttacgctga
      661 caggatgcgc tggctactga cctggaaaaa gtggccaaga cgggttgtct tgtaagaaag
      721 agggaggctg gtgatgtata gtctccttct gggacctttc atggaactgc tgttggagac
      781 gccccgcaat ccctctctcc gctcatcctc tgccttttct ctctctctca tcccactatc
      841 tatctctgtt ccccagtcta gtctccgctc tcatcccatc tagtcagtgg tggtttttca
      901 tgaatctccc catgccatgt ttcccccacc cagtcaccga ggtcattcat cattattgtc
      961 aagggggtaa tgtgtgtggg tgggtgcaga gatctatgac ttcttcattt gaatgaacct
     1021 cattagattg actcctcttt gtaaaggatc cctttagaaa ataactctgg cagttattta
     1081 ggtgattctc acatgatagc caatggaaca tgacgcttca gccaagcata tttgctggaa
     1141 cgatggaaga gtctctgtga tgcagtctgc aggttatatt ttgctttatc attctgccct
     1201 gcatacctac atttacatag caatgtagtg ttggaccttg agcttgactt gtggtgccta
     1261 catgctgtca atatgatgca atctattatt tttactgact ccactgatca tttacaacta
     1321 tgaatgagcc ttttgtacaa ggacaagctg cttaggtttt tggatttagt cttatttagg
     1381 ca
//
LOCUS       KCVB01000085            1409 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3842pri ultra-conserved element locus
            uce-667, sequence.
ACCESSION   KCVB01000085
VERSION     KCVB01000085.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3842pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1409
                     /note="ultra-conserved element locus uce-667"
BASE COUNT          444 a          289 c          311 g          365 t
ORIGIN      
        1 ctgctatcca acatggacta gatagcatgg gaaggcatgt cagagagaaa aacaagggag
       61 agaatggacg taagggaaga gtgatataaa cggggtggag aaaaaggtgg cagagcacgt
      121 gttgaacaca cagagaagca gcaagaaaac aagaccagca gtgcgcaggg gaggaggaag
      181 tagcgagatg aaacaggacg ggtcgtgcct gtcatgatta cactggcact aacgggaaca
      241 tttggatgag atggaaaaca agacgccatc tcgtgttcca ttcatgtctg cagcgccttg
      301 gcctctgttc cctatacata catgcacaca cacacacaca cacacacaca cacatacacg
      361 cacacgcacg cacgcaccca cacacatgta cacagtcaca caaacacaca ctggcagagc
      421 gaagcaggag cttgtggttc cagtccagtg acagcggcct gtagtcaggg tggcgacgct
      481 tcaggagtag ctcctcgtat tggagaagca tgtttgtgtt ggccctgaat taataacttt
      541 tcatttagtg cggaaatgac aggaatagta attaaggaaa tggaatgatt ttattggcca
      601 tgatgtttat attaagttcc agatgtgaag cagctgcttg cgcagcaaga ggccctgccc
      661 cccatgtgct atctagagga actcttttta aaattaagat aacagcacca gttaaatgaa
      721 tgtttgtgtg tacaaacaac atagtatctc attcacagca tgtagcaaac tgagctaata
      781 tcatgtccag tttgattaat gggggacagt tatgaagcac tgcgaggcct gctctgtttt
      841 tataaatgtg cccctaaagt gcaatttatt tgcagatata atgttactct atttcagacc
      901 tgatttgcct gactggaaaa gttaatattg aggagtggct gttcagcatt tttgggtggc
      961 cccaacacgt gagatccaca tcatattaaa gttcagtatc ccttaaatgg taatgacagc
     1021 attccttgga ttctgacatt gcctgtcaca aagccagatt ggatttcaaa tttagatcaa
     1081 gccaatctct actgtaactt ttgaagctgt tgtcaggctc atgacaagca ctggcacaga
     1141 aatgaacaga aagactacgt ttcctttgcc ttaaccaaac ctagaggatt gtaacaatat
     1201 gtacctaaac ttcaaaataa tgttcacacg tactctggaa ccttatgttc ctgtattgtg
     1261 caaatatttg atattctctt gactgataat gtacattcaa tctagcattt aattatttta
     1321 aaagcccctg aagtgttgct aaagtgcaag ctaacatacc ttactttagg tttatacaga
     1381 catgaacatt aaacatgaag ttacaactg
//
LOCUS       KCVB01000086            1605 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3885pri ultra-conserved element locus
            uce-622, sequence.
ACCESSION   KCVB01000086
VERSION     KCVB01000086.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3885pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1605
                     /note="ultra-conserved element locus uce-622"
BASE COUNT          429 a          360 c          328 g          488 t
ORIGIN      
        1 cacatgcttg tgtccagggg ttcccatact ctttcatgtc gaggaacccc accatagctg
       61 catattagac cacagatcct tgatttcaga ggattttgtc ttaataaatc ctctctgaat
      121 attttttttt tcatttttag tcttgaagat aaaagagtct ttatatttaa tcattgatca
      181 aactaggatc acaataaatt acgatcaata caaaacagcc aaccatttct tgccttgttg
      241 ctccaacttg cggaaatgtg atgaaagtta aatattctat accttctttt gctgaggtcc
      301 ctctaaaact cactcaataa accctgttgt aagtagttaa aaaaaacctg aatgataaaa
      361 tctctctaat atttccaaaa attcccaaaa gggatttata aatggtgtta atcatatttt
      421 ttattttata taaaatgaca catttataaa catataatta ataacataca aatatgcccc
      481 tttatagttg atatttatat agtaacactt taattttatt tcctatagtt ttcctatggg
      541 atacacctgc gtcactaaca gtaagctcca tttccaaaag ctcagattgt tcatttcctt
      601 atggggaata aatagggggt ggttgcttct ccagtcgata tctttaattg ctcctggctt
      661 gtatcgatac ctaccggttt tactgctcca tcacgagcca ctgggaacgg gttgcccggg
      721 aaacggccgc atggcaacag gatcagagcc tgggggttgt ctgcttctag gtctgctccc
      781 attttacatc gcatttcctc actagatggt gtgcaagcca ctggagagta cacttccccg
      841 cacagtgggc tctacatcca gcaaaacaaa cctgggagac tacgcggctg accatttctt
      901 tacggggctt cgaggagtaa aagtgtggaa ataaagcgtc gtgagcgtta aggttttctt
      961 tttataagcc aaccccacgc tttttgcaac aagaagactt gggaaaagcg gcaacaaaat
     1021 acagctgtga tggacataat gattgcggtg caggatgctt gcgtctctgg tcagtggctt
     1081 actgcgataa ttctcagtct gtgctgcttt ctgccgtcct gtttgcccgc tggacaaact
     1141 gtggactatt cctcggttga aagtgtggtc agcagacaag gcgacacggc tctactgagg
     1201 taaaacaagc taacacaact tcactttctc ccgctttcac tgactctctc tctgtgtcgg
     1261 agttctccga agctaaagcc tcgaccgcac acgctcttta aacgagagtt catcatttta
     1321 gagatgccta ccaactactt ttaaagacgg ggtgatcata cagcgcttgc gatattacac
     1381 aataggccat tttccctcca tcccgtcctt gttttcgcct gacagggggg cggctagagc
     1441 cgctacgcag gacgctgctc ttgcgacaag tgtagctcgg caggttagct actaacggca
     1501 aagttttcta agatactttc cctttgtgta catgagctaa cagagaagat gtgttgtgtt
     1561 gcggtgtgtc tgatttcata gctgcccgta ggtgggacga tattg
//
LOCUS       KCVB01000087            1239 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3927pri ultra-conserved element locus
            uce-1178, sequence.
ACCESSION   KCVB01000087
VERSION     KCVB01000087.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3927pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1239
                     /note="ultra-conserved element locus uce-1178"
BASE COUNT          329 a          293 c          300 g          317 t
ORIGIN      
        1 aacatttcat aacagtggag gaagccaccc ggcattctcc tctttgcctc ccatcacaca
       61 gacttggaaa ttagctgaag gaaatagcct tcacttccac tgcagtaggt agcggtcggg
      121 gctattcagt ggaaatgctg ctatccggtt gcaacaattc aatttgcaat gacaccagta
      181 atttgggtgt aatgggtgga aaattgagag ggtaaaacta ggtgagtggc agagagagag
      241 gagttaagga gtgaggagga gaggagaagt tgaggggata atgtgaggct gaaaaattgg
      301 cagaggatcc cctcaccacc tctctatgtc catgtatgtg tatgtgtagg cttcatggag
      361 gtcagccacg tcccttccag acctgacatt taactctgac acctctcatc tcatctctct
      421 gcctccctga gcctcccacg gcttaatgag cgacacaatg aggacctcct tccctctctc
      481 tatctgtcct ttccttcatc tttcttcccc catcctctca ttctcatcct ctatcctctc
      541 tcctctctca cacactcccc tccaataatc cgcgtggtga gtgtgtgtta ccgcatgtgg
      601 atatgaggcg acgaggccaa tgtgctgctg gcagccaatt acattaatgc gctctcttct
      661 ttgaactgct tatcgctgcc agcgcagcca tttccaaaca ctggggctct gtcatgcctt
      721 tccaggggat gactcctgag agcccgggct gggcccgagg ttggggaagg ggagacgcac
      781 gtgacagtag aggacaagca gacacacaca cggagcatca actccggcct acacacaagc
      841 agacacagta agtggccaag tggaaatgtt ttgaaccaac gccagcagga ttcctgtaaa
      901 tggaaaaaag tcatgcataa taagcttggg tttaaagggt gaagtcttcg tatgagagaa
      961 taaatgtgtt ggtgggtact ccaaatgaaa ccgaattttg aggttggcac aggcgaaatg
     1021 gcaggtgtac ctctaaaaga attgttgctc tcggctttaa ttgccatagt gctggcgggc
     1081 gggcagagca aactgctcaa aattttccca caaccatcga tgcatcattg tctgcttcag
     1141 gtcagtgctg tcatatgatg ctgtgaactg tttgtttgca agttatagaa taacttgtaa
     1201 agaatataga atatagaata ataaaaccaa attataatc
//
LOCUS       KCVB01000088            1586 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 3969pri ultra-conserved element locus
            uce-909, sequence.
ACCESSION   KCVB01000088
VERSION     KCVB01000088.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="3969pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1586
                     /note="ultra-conserved element locus uce-909"
BASE COUNT          426 a          342 c          333 g          485 t
ORIGIN      
        1 ttttgtagca gtgcattttt aaagtgcatt gaataacagc tttatttaag taatggaaaa
       61 tctacacaca gtaaaattaa taagcaaatg tatatgtatc aaactgtaaa tgcattatac
      121 taataacagt cgcctgtttc atcaggagac taatggtgtc cttattagca taattaatga
      181 caaatggcag cgcagcattg gtggattggt tctaggggat gataaattgg tggatgccca
      241 ggttagtggc tgccaaggag gcctctttgg ctgtcatccc tcctgcttcc tctctggcac
      301 agttcattac tcaccgtgtg agatgccact ttacattgcc tggtactgtg agcatggaga
      361 acttaataat gccacccata accacacagg gcctgctgct ttacccacag tccagagggc
      421 aagaagctca gcgagggctt ggggttgcag ggagcaggga gacatgggaa tcatctcccg
      481 aagctgcact gatcaaaaca gatcacacgt cagtcactac ttgaggggaa ccggggtcat
      541 atgacccagt ctcctgtgct ggtgagtgtt tcttgttagg atttcctggg ctcgccacat
      601 ccctcgcatc attacgggtt agtttgccca cagaaccgct aattaagagg ggatttgtgt
      661 aattgtgcct tctgctgtgt cccatccggc acatgcaatt tactgtcagc cctctgccag
      721 tttttacact tgtccagaga ggcgtacccc atttatacac acattctgct gcacatttac
      781 cagcagccac agctggggat caatgccagt gccatggcaa tgtcttaaaa gacactgtgc
      841 tgctgattaa agtctgattg tccatttaat ttcatgagag ctctcaatag ctagccagtg
      901 aacagggctc ttccagtgct tcttcagcac tgactgctgt aaaaacagct gatacaagtt
      961 tttgtaatcg taccaaatca ttgaagactt ttggtaaatt ctttacatta aatagctctt
     1021 atgtgtgtaa catacacaat gcttatattg gagaagttat cacacatgta cacacctcct
     1081 cagagcagtc gctgttatgc agcggtgttt gtggccaact caccgtaggt gttgctggct
     1141 ctgcccactt tctggaagac tgatggacca gcgcccgact gggactgcct cctggctccc
     1201 tacccacaac cagatttcaa ggactgcttg tcacagatgg aaaagtaagt gggtcattct
     1261 acataagctt gcaattttgc atccctctgg aaacaaaaaa aagttcaatg ttattttagt
     1321 gcagatggcg tcttattttg tgtttgtttt ctttttttta atttgtgaga gctcatgttc
     1381 tcattttggt ttttgtcata aaaaactttc aacgataaaa ctgttcagcg ctaattacaa
     1441 gtagaccatt aaggctgttt tatgtagtta ttgcttttat acctgattta atatgtttaa
     1501 tacatttcac aaaaatcctt taaaatgagt taatacactg taagatgtta caccaccttt
     1561 tctagctgca atggttaaaa aattat
//
LOCUS       KCVB01000089            1507 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4014pri ultra-conserved element locus
            uce-566, sequence.
ACCESSION   KCVB01000089
VERSION     KCVB01000089.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4014pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1507
                     /note="ultra-conserved element locus uce-566"
BASE COUNT          465 a          283 c          298 g          461 t
ORIGIN      
        1 gaagtccacc tcagggtctg tcttacagcc ttttattttg tgaagctctc ggccactcct
       61 acatttactc ttgtctcttg ctttgtcact ctctttttct cctcttagct tcctttttag
      121 cagtcacagg ttacctcaga aaactcataa atcacacaga gtttaggcag agaagtcgga
      181 gaacatcaca ggcaaagcct gaaagcactt tttccataaa atatgatgta gtttcaccaa
      241 gaagggtatt agctcttgct gtgtaagagt gctataaagg gttacgaagg gcttcctacc
      301 tgctcctcca aattatcaag tatagtaata ggcaatcagg gtgcagagtg actgacaaag
      361 agacaccatt cacactatca caagtcataa actcctttct aagaaacaaa ggaactcaga
      421 aaatcataga taattacgtt atcttttgga cagttaaaga tgttactttt ccaagagact
      481 ttcagtattg tataataagg aaaatacaaa ctctatatgg tttttctctc ttgatgtaca
      541 aaatttgtgc accattaagt aacaaacatc ctaaaacact gtgaaagcct gatggctgaa
      601 tgtacagtgc ttgcattgag ttgtactgat gtgtatctac atggacaaac acagagacag
      661 taacagaact ctgtgctccc tggccttcga gtcatctcac tacatcaata aatacagcaa
      721 aatagcatta gcaataactt caactgtatg accaggctat aatatgatag aattagtatc
      781 agtgttttaa ggtttggcca tttaggtgaa atcctaagcc gggcagggag taatttggac
      841 acagggaaaa ttgattttga ccatcagcac tttctcattt tcaaacagcg cctgcggcta
      901 aatgggttag cctgttaagc acattacaca ggtagcttct cctttcctcc ccaatgatta
      961 tgtgtccgcc ttgagcaatt gatatttctt gcaaataaga aaattatcat attccatgac
     1021 tggaaagaga aattaaggtc tgctgttatg gagtggagtc tcaggactga gggagggaga
     1081 ccaaggaata tttttctttt cctttttttg ttttttaggg tggggtgtat gtatcaaata
     1141 tccgaagcta tattggaatt gcagcgctgt caggcctcat caataaaaat atacagtgat
     1201 agactgtaag tggtgtaggg gggacaataa gggatggagg gacactgaga cagaaggaca
     1261 ggcttttact ctgtgctgag gcagaaagag ctgggaacat atacagtagg gtgtgtcata
     1321 agaaggctgt aatttcactg ccaaattgtg cactggtctc ataaatcata aaatttcttc
     1381 tctgaatttt ttttactgct gtgtctgacc aaagttaaat aagcatttaa tctaaataat
     1441 ctgtagtttt tttatcattt aattgacaca cttatcttaa aataactaat aacaaccaga
     1501 ttgcttc
//
LOCUS       KCVB01000090            1055 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4057pri ultra-conserved element locus
            uce-523, sequence.
ACCESSION   KCVB01000090
VERSION     KCVB01000090.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4057pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1055
                     /note="ultra-conserved element locus uce-523"
BASE COUNT          308 a          193 c          263 g          291 t
ORIGIN      
        1 cacagaagtg cagtctctgg aggttgaagt ggtggatgtg gtggaaactg acaaagatgt
       61 cctaagtcca tgtgagggct tccatcctga tgatattaac atggctggaa tagagctaga
      121 ggacctgttt ggaatcgaag acttaaaatg gacacttgaa agggatactg cctccccact
      181 ttttgatatt gagttggctg atctcggtgc gtgcactacc aatgaccaag attctgggat
      241 tgatgcttca ttagcttcat ctccagagag agtgttatct gacttgagga gtaagaacag
      301 acataaccag tcaagccaaa ttatcaacaa aaatgccatt gctgccagat tgaatcgatt
      361 gaagaaaaaa gaatatgtca acagcttgga gaagaaagtt ggcatcctgt ctacagaaaa
      421 caacgtgcta aaacaagaaa attctcagct gaccaagaga gtggaagagc tggaagatga
      481 gaccaggtac ctgagggctg tgctggccaa tgagagcatg ttagcccagc tcttgtccag
      541 gctaagcggt gtgaatggga tgaaattatc ttcctcactt ttccaggggc ttgactcaaa
      601 cgaccacgac tacgccttac caaggaagcg tgtgaaagtg gaggaaaaag agacatctgg
      661 tggcgtgtgt ctgcatgtgg acaagaatca tgtctcggtg gagttctgca ctaagtgtgc
      721 agaaagtgca agcacatcgc tcaaaatgta gggtcaagta cctatctctg ttttcagata
      781 ttgagactga acttgataag gctcagtgta ctgaacttct tgagggtgga tgaacactgt
      841 tgaattagtt aaacttatct aagaactgct caatgtgcat gtgtatttgt gtgatgaaga
      901 attgagtaca cgttactgct aaaccttgtt gacagtttct tctaggtaat gattgatcca
      961 ccatgcggga tgagtggctt tctgaacacc tttacctgct tgactccatg gtaaggatca
     1021 gacaagaaaa ttcttttttt tttttttttt gtttt
//
LOCUS       KCVB01000091            1098 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4146pri ultra-conserved element locus
            uce-1185, sequence.
ACCESSION   KCVB01000091
VERSION     KCVB01000091.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4146pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1098
                     /note="ultra-conserved element locus uce-1185"
BASE COUNT          323 a          248 c          240 g          287 t
ORIGIN      
        1 gagagagaga gagagagaga gagaaacatg aaagcacgag cgaggggggg acccttgcca
       61 ttctaaggca ttcccatgac tgacagagag agtaaggttg tgtatgtgtt tctgtgtgtg
      121 tgtgtacatg catgcgtgtg tgccattccc ccatcctcac cccaccccta cactcaccat
      181 tgtgtgtacc acctctgtca cctttcctct aatcaggagt ttaaatcaat gacgttgtcc
      241 aaaactataa ttgccactcc ttaaactgct tctctcgcaa tatgctttca ccaagaaacc
      301 actcggcagc agaaatacgc ctctgcgtgt gttggtgtgt gtgtatgtgt gagctggatt
      361 aagagtattt tctcattacg gcagtgtctg aacgagtaga aagtgcttct cacattgttt
      421 tacagcatcc tgaaacctca aagctcagca ctaagcagca taatatcctg tagtcccaca
      481 cagacgcctt agcctggaac gctgtcgtca caaattgttc atgatccaaa ctcgttggac
      541 gtcatttatc aaacttttta agaatgaatc attcctatgc agaggcacat acacaaaaaa
      601 gttattgaga gactgatttg ttggttacat tgtacctaaa ttcacatttt gttttggtcc
      661 ttgtgagtcc agactgtcag agtagacacc agctcataca ggcccataaa gtgctcgaaa
      721 ggacccacct ttatggcctg tgtccttctg aggatcagct tctgaattca gatacagcat
      781 gacaaatagg aaatggtaaa tgagcgagta acaatgcaag gtgctagccc caccatcagg
      841 agtgactgag gtccagtgtc ttgctcaagg acccttgaac atgtggactg gaggagtcgg
      901 ggactgtacc actaaaccaa tgattaatgg atgacctgtg ctacctgctg gggaacagta
      961 gccatacaca tgcagcaaaa gaggggcttt tatgatgctg tatatataaa caactaaaac
     1021 accggctaag caaagctgga gctaaaaaaa agaaacaaat cttccatctc taacatcgta
     1081 tttctgtaaa atctaaac
//
LOCUS       KCVB01000092            1518 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4183pri ultra-conserved element locus
            uce-1244, sequence.
ACCESSION   KCVB01000092
VERSION     KCVB01000092.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4183pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1518
                     /note="ultra-conserved element locus uce-1244"
BASE COUNT          406 a          332 c          341 g          439 t
ORIGIN      
        1 tgctctggtc gctgcagcca tgacaagatt cgcaattctc tccctttatt tacacaccat
       61 cgccctggct ccgcgtcatg tcagagtggt gtggttgcgc gatcgcggct gaccccaggg
      121 taggggctgt ctccactgat ccaagcttac tctgctgggg gaccaggccc cggtcatgta
      181 atacacaatg taacagcagc agcatttcat gccatcatac aaatatcctt caagaaatta
      241 aaaaaggtac cttttcaggc tttgtgatag tgctgtataa aaaaaaattg tttatgaaaa
      301 tatatatgat aatatttaga ataaacttgt cctacgaatc tgtgaatagt tggcctttta
      361 tcttcttatc ctttaataca acagtgggat cttcatcaga cgagcagaaa tacactgcac
      421 ctttatatag cgattgcctg gatgttgact acttttttca cagcagacag tcacataaga
      481 tgctctgctc acaagcaaat gtattaccaa accgaaaagc tgttggaggt gtcatgtaaa
      541 tacctaacca cagttaaagt aatagcctat aaagtgatct ttaagtggag ttttaagcac
      601 ccccataatg gctttggctg ccttttagca gaccatgtga ctcccccaga gtagtgatta
      661 tgagtcacgg atgaagaggt cttgtgtgta ggtcagcctg taagcctcac tttcacctgc
      721 caatctttac tttcctcgat agtcggagag ccttgttctt tttactgtta actgtcttac
      781 agcaggaggt atctggtaaa ttatgtctac aaaacacttt gacaaaactt gggtatagcc
      841 ttgtggaatt cctactgtgg agaaaagcct ccaaaattga gagacagaag ggaaaaaacc
      901 ccagaaatta aacagccttc ttgtttcgca aattgtggca tagaaagaag ggagttactg
      961 tatatgtaag aattttccag ttgtgtttgg ggagcaaaat tcctagctct gctcagggtt
     1021 ttcccctctt acattacaca aggacactga tgcatggtgc tttttgaatt ttgtattatg
     1081 ttagaggtct gggggtgcgt ttcaggaagt gttcttaatt tcacagggtt aaggcacagc
     1141 agattcacag ggaatacaga tcacttagca caagcctgcg accgccctga agttggcgag
     1201 ctttctcccg atttgttaac cacatccttg gggccgctga accgttgaga ctgccaaggt
     1261 gaacgtgcag tcccagaagg aggctttgct gtcatagatc agtgaaacgg tgattcagct
     1321 tcacccgtgt gtttatgtga gttcacttgt gcatgttctt ttagtgtatt aaccctcaca
     1381 gcgcgtgcgt gtgcgtgtac atgtgtgaaa cgtgttctgc ctgtgtgaaa agcctctcca
     1441 gccacgaggt ccacgagtcc tcaggcctca tgatcaaagc agggccatat gaactgtgcc
     1501 ataacagatg tctacatc
//
LOCUS       KCVB01000093            1976 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4226pri ultra-conserved element locus
            uce-276, sequence.
ACCESSION   KCVB01000093
VERSION     KCVB01000093.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4226pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1976
                     /note="ultra-conserved element locus uce-276"
BASE COUNT          530 a          498 c          419 g          529 t
ORIGIN      
        1 agtacctttt ggcctgagag cagatcacat acttttagtg tgtcatatgg tgtttggatg
       61 aaatcattat cgtggctgtc gccaccatca catcatctag taaagcacca cttttgcttt
      121 attgacttag cccaagacaa agcacagagt gagtcaactt tgaattgatt gtatgatgca
      181 ttaggcttct agcctgagga agtacttctg ctgtttgaga gttgctactt tggagaatct
      241 gaaatcccac aacagctata attgcaacag agatgagata tgtgatgtga cgtaaaaccc
      301 aaaaatcact atcagtcact gattctcatt taaaatgtat cttaattcca aaaacaagct
      361 aattcttcaa gcttatatga ttgacacaat tttattagcc atgactagaa cttacctccg
      421 ggtctgtaaa caacatcatc ttctgtgatg aaggacgtga tctctccgtt ctctgtgcgc
      481 tcgtagcgcg acttcttctt cggcgtcttc ttcttgccct tcttaccggc ctcctcggcc
      541 gtgccgctgc ctccgcctgt gctgccattg tcgttgtcct catcctcgct gtgctcgctc
      601 tctgcatagt tcttggcccc gccctccaac gtacagctgc gtctgggcct ggagctttca
      661 ctctcccggt cgccccgctc tccgtcctgc cgcctggact taccagcctc tcttttgtcc
      721 cggtccctgt ctctgtctct gtcccgctct ttctccctct ccttctcttt atcggccgtc
      781 atgatccgcc acgtgccttc ctcagtcctc tcacggctgg gcctccgtga aaggtagact
      841 gtgagcctgg gcttcagtct tctgaattta ctagtcaaat ccagggaaaa tttggccaca
      901 ccaacaaccc cagtgtttca gaaaagctgt ggacaagaga aaagaagaac aattagtata
      961 actagatgta caaaatcatt atccttacag tgcagaagta caaaggtttg tcctgtgttg
     1021 gaggagattt tttctgggtt agaaatacaa gattagctcc tagagaaaag gcagctgtgc
     1081 tgagtcttct caacatcaca gtaattgttg gcactcccct ctacaaccat gtatactctg
     1141 gagaacactg agtagcttta tcaaagagca caaccttcta gcttcctgct cagacatatt
     1201 tccattcttt cagaaaggca aaacacagag ttattaaagg gtaaaagaat gtaaaaaaaa
     1261 gagagtgaga aaagtagaca gacaagtgct gtacgaacag tatttgaaac aaaataagct
     1321 taagggaaga agtaactgaa taagcattaa aaaaggtagt gctgaattga ggatgaggca
     1381 tgcagccaac agaattcctc agagataatg tgtcgagggg cttttcggca caagacgctg
     1441 cttgtctttt gcgatgccac gggagagaca cagcgtgaag ctcaatgaaa gagttaactt
     1501 gggagcttct ctgcagacca tctgtgatga caccaggctc aattttactc atctgtgtgc
     1561 ggttatgtgt gtgtactctg gcttactgga ctgcacccca ccccaaccct ctgccctgcc
     1621 tgacaatcat cactgtgtta atgtgaagag acagcactgg agggaagaac acagcaatcc
     1681 actgggtaaa gaaagggaga ggagagaggc tgagccagtc atttgccatg gccagtccac
     1741 tgccattttc agctatcgct gttcactcaa aaaggaactc ggccaccact ttctcatgcc
     1801 ataatgaaat cctccataaa cagtgaagtg ggcaaagctg aagcagcaca ctgtctgcac
     1861 tacactgccc tacacacaaa ggaggcttct catttactgc cagtgtttgt cccctccacc
     1921 cccaccgcaa caaattaact agaaatagtt attacagccc ccagagggat cagacc
//
LOCUS       KCVB01000094            1205 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4268pri ultra-conserved element locus
            uce-934, sequence.
ACCESSION   KCVB01000094
VERSION     KCVB01000094.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4268pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1205
                     /note="ultra-conserved element locus uce-934"
BASE COUNT          399 a          222 c          191 g          393 t
ORIGIN      
        1 aaaaaaaaaa aaaaaaaaaa aagtcattct aatataacac agaaaacaaa atcattaaat
       61 gacataacct gttaaaaatg tgcactgcct gctgctgtgc aacaaaatac atctggcttg
      121 actgaaataa tgaaaaatag atttcttagt catatttctg atctgctgcc atgagtgaac
      181 aataccttta ccttctctca gtactcaaac tttttaaatg tcctggagga gttcttgttc
      241 aagtcccaaa cctactgaga tctctaatga aggctttacc ttccaggcaa gcatatttct
      301 ccaaatgaca gtaatttaca tatgatgtat ttagagaaga agagggcagg ctaattctat
      361 ttagagattt tcctttgttt taggagtcat tagcagctag ctcaaatcta atttttacaa
      421 tttcatcagg ccatcttgga aagcctctgt gagctcatca gagaggagca tttattgcta
      481 agtgttctct attgcattag cacacaataa agcggccagg gactgctaca gacgtttatg
      541 tggaatccag ttaacactcc gtcaattcgt tttttattgg gacacaatca atttttattc
      601 attttaaagc cagaatgctt tctttcattg tgtgcctact ccagcttatt atgccctaaa
      661 ctaattactc agttgttttc acaaaatgct aatgtttatg ttaaagattt cctccttgaa
      721 atgcaggcct gacgtgcata tacattatgt gagattttct gtcacatatg ttcttcatta
      781 tcacacactt cccacaatgt ttttaagcct aataattgta ttcatttaat ttgtcgaggt
      841 ctggcaataa caatgaagtc tgactaataa ctcatagtgt ggcaaattaa ttttgatgaa
      901 atatgagtca agtctggccc tcaaccaaaa ttattaggcc cttcttttga aaactgaatt
      961 taccctctca gggctgcaac aaaactcttg gcatattttg aaaatttact gccgagagaa
     1021 ctgaaaaaaa gtccttttag gtcctgaagt aatcgtgtgg aagcaaaatg agaataattc
     1081 tgtattatgc aaaaagctat ttgaaagttc atacaatgga cagactacaa actgacctgt
     1141 atcttttata atcaaacata aaactaacta ctacagtttt atttgactaa aagagatttc
     1201 agttt
//
LOCUS       KCVB01000095            1386 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4311pri ultra-conserved element locus
            uce-132, sequence.
ACCESSION   KCVB01000095
VERSION     KCVB01000095.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4311pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1386
                     /note="ultra-conserved element locus uce-132"
BASE COUNT          407 a          282 c          280 g          417 t
ORIGIN      
        1 gttgtgtttg tcatttgatt tactagtact tgttgtgcct cttaagctta ttgaagttac
       61 acagccttga cttaatgaga tttatggaca tgctgtgtag ttaaggtaaa agcaaggctg
      121 tcaactgaca aacagggcaa cagttctcat ctacattttg tccctctcca tctcctacat
      181 ccattagcta tatgttgatt ttcaaaatgt atgcttttaa agtcatttgc acatttcaca
      241 tctgatttta ccactggttt caacaatgat tatattggct tgcaaacaca tattcaaacc
      301 cccttcggca ggttttagca tttcctttgc attgttaaat caggctttac tgcatatata
      361 ttgtgcacac tttcaaagta aaggaaacgc aaggcttctg gcagctattt ttggcgtgtg
      421 gggtgcattt tgttgaagct tatatgcaca ccaaccttta aaatggaaaa ttcgtgccaa
      481 gatttgatta taaggatttc ccttacgcca acaaaactct aaatgaggca atttgtcact
      541 gaagaattat gtcaaatcca gatactccaa ccattgcaaa ttaaaataat tcaggctttc
      601 agtgtgtatt accctaactt taattatctt ggtgtttcct ttattttggc agtcccttgt
      661 gtatatgcag agacaagtag atttgtttca aacctggcct ataagtaaac taccatgaca
      721 ctaacaaatt gatcagagtt ggagggggtg gagaagagga tgcatttcca ttattggctg
      781 tcaataccaa agaaaaaacc cttgccattg cagaaagtca tgcacaccag gaaaagacac
      841 atggagctgt tccaggaact gaatcagaaa tttcaaaccc tggaccgatt tcgagacata
      901 ccaaatatgg gcagcatggt gagtaacagg aaaccagggg ggtggcggtc agccaattaa
      961 agagagcaac atccaaatac cctatccaga tatccggagc aggacacata atgtgtgatt
     1021 gtgcaaatga gcagttatgt agctgaagta ttcatgtgga agtgaatctt cctacacaca
     1081 gtgggcctgc cagcagctgg cgggggggcg agtcagggtg cagcccctta aaagctcgga
     1141 agcattttct ccacaaggcc agtttaaggg aagaaagacc tttggtaatc ttattgcagt
     1201 agagtgcatc cattccaatg caaggccatg aatgcacttt tatttacatg cacatttatc
     1261 tacttagcaa ccccatcatt tttccattaa tgcatggcag attttttaat ctatcaacat
     1321 ctatacaaat actcccttaa gttcactgtt cacttactgc aactgtgtgg ggggggggct
     1381 tctctt
//
LOCUS       KCVB01000096            1583 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4356pri ultra-conserved element locus
            uce-1058, sequence.
ACCESSION   KCVB01000096
VERSION     KCVB01000096.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4356pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1583
                     /note="ultra-conserved element locus uce-1058"
BASE COUNT          481 a          347 c          302 g          453 t
ORIGIN      
        1 ttaaatgatg tatgacctcg ctgaccaaca ttaataagtg tctggcaccg ctctgtgcac
       61 accagcaaag atcatcctcc tgaacaaatg aattatgaat ctgggggtgc aggtcaagac
      121 taaatatatg ttcagttaat ttgaatcctg aaatgaaaaa tatgaagctt tacagagagc
      181 caccaggccg ttaacacaaa tcaaaaccat gggtaccaaa ggggaacatt tgatttgtgg
      241 tttttgtgtc tgtctcgctg acattgccaa taaatagagc atccattcat tataacctgt
      301 cttgttcatt tagtcacatt taatatccta gcaagccctc ctacacctcc ctccattcat
      361 cccccagagc cacaagtgtg cgtttgtgtc tgccagtgtc tgatccctca cagctgcgta
      421 ctggtgcctc ccaaccctgc cttcgctaac tgtcttcagc atgactggat gtggtggagc
      481 acaagatata gcacaatata tgttaaatta ccagcgcaag tgtttgtgcg tgtatgtgtg
      541 tatttctccg tctcaatctt gcagcccctc cacccaccga acacacgagc acacccaccc
      601 acccactgac ccctccctct cagcgctggt tggttgccac ggctacagtg tcgcctctct
      661 tcctcatgtt gctatgagac tcggaccgac agacctgagc tgaatgacaa tgtgtctgtg
      721 gctctggcaa gaggcacaca acagccaata agagacggtc tagacaaaca cttcccaatc
      781 tggggtctgg ggacctcggg gtgattgttt gggttggcag gatcctcctc agccatatga
      841 ggagcagttt attaacacta ttttttttca tttccttagc aattgtctca ataagaaaac
      901 atgtcagtca ttggaggcta aatgatgaga gttcctgata aaaacgaaat gaagattctt
      961 gttgtacacg gggagacaat agtgatgcaa tgttacagta attatatgaa ataaggatat
     1021 ttaaatctgc attaatcaat atttttttta tattaacaac agatcaatga ctatgtgtaa
     1081 ggtgaaaggt gcttctcaaa gtgataaact cacagagaat tatcaagaac tctgcagttt
     1141 ccctcaactc tacaaagctt tttagctcat tttagctcac tgttttggtt tcaagcctgc
     1201 agctgctttt cagctgaaaa ctcacctaat caccaaatat caaactgcaa actgacaaat
     1261 ttaggaatta actggtgaag actgtggaac atctagcagc taaagagagc atgatggaaa
     1321 ccaaacggga gctaaaagaa gagtgaatat ttgacttaaa tgtgtcttaa tgatagagac
     1381 atcctaaacc aaaatcattc aaataaatac taatcttgca aatattaata gaaagcattt
     1441 ttgccataat gcaatttaaa aaggtgataa tatgacagat gggtttttga catattccgt
     1501 tcctctcaag tggccaaaaa ttcaattaat gcatctttca ataactgttt taattgtacc
     1561 tgatgttatt ggaacaccta acc
//
LOCUS       KCVB01000097            1696 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4402pri ultra-conserved element locus
            uce-702, sequence.
ACCESSION   KCVB01000097
VERSION     KCVB01000097.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4402pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1696
                     /note="ultra-conserved element locus uce-702"
BASE COUNT          451 a          380 c          378 g          487 t
ORIGIN      
        1 gtggcgtgca gcattcatct ttatgcaccg tgctctgtct tgaggcgttt acagctcaac
       61 aaatgcatgc actgtttggg ctcaacgcac tctgcattgt aacaatttga tcgataaagc
      121 accgtggata aacgcagagg ctggaagtta ttcagagtat aaccacggag tgaaaaataa
      181 gactgcattc tgtgggggtt tacaggcaga caatgcaccc tttaatgctt cactgactcg
      241 ctctgctcgt gcagatttga ggctgaagaa ttattatttg gagctttcca attcaaaatt
      301 tcagcagaga gttcaaatta gccgaaagat tctcttattt tactatgctt gctttaaaat
      361 gttgcatatc ttgttaaata tcggtgtgta attccgtttt gacaagtgct taatcattaa
      421 caggttcttt ttcttattac gcagcagcac aattaatttc atccaaactt ttgattgatg
      481 attatattgc ttgaacttgt gataaatgtc tctgggaact ttacaacata acattttgac
      541 agtgtttctc ccgtttcttg ccgcagtgca cggcctatcc gccagctcac aggactcggc
      601 caagtccccg gagccgtccg cggacgacga atcgccggac aacgacaagg aaacttccag
      661 tagcggcggc agcgactccg gcaagaagcg gaaaaggagg gtgttgtttt ccaaggcgca
      721 gacctacgag ctggagcgcc gcttcaggca gcagaggtac ctgtccgccc cggagaggga
      781 gcacctggcc agcctgatcc gcctcacccc gacccaggtg aagatctggt tccagaacca
      841 ccggtataag atgaagagag cccgggccga gaaaggtatg gaagtgaccc atctcccttc
      901 tcccaggcgg gtggccgtgc ccgtcttagt cagggatgga aagccttgtc acactcttaa
      961 agctcaggac ttggcggcca cttttcaggc cgggatcccc ttctcggctt atagtgccca
     1021 gtcactccac aacatgcagt ataacgcgca gtacagcgcc gcggccacgc cacagttccc
     1081 cacagcacat cacttggtgc aaacgcaaca gtggacttgg tgagagaaat tatagagact
     1141 tggcttggag gcacgatagg gagtttcacc acaaagcaaa gaaaaaaaaa taaaacaaaa
     1201 aaacaaaaga cttcattttt gcagcttgac tcatatatat actaccattt ttattcgggg
     1261 ctcatgtgtg cgccgtgttt acatgttttc gttaagagaa ctgggtccaa acctctccct
     1321 tatagatttt attaagaatg tcaatgctct tcttgtgaaa tttttttgta aagtatgttg
     1381 tgtgttggtt gtagagatgt tttcctcttt ttttcttttg tcccttcgtg ttattatcag
     1441 cacgtacgaa ccactttata aatatagaaa ttttaatttc ttgcttcttt tatggaccga
     1501 aaaaatattt atggccatga ataaacactg gcaacttttc agctattttc cttttgtttt
     1561 tatttcctgt aaatattttg tggcacatgt tttcaaacct agagagcttt tcgggagcca
     1621 aagctggaga tcggcctgtg caggctatat ttagaaattt caaaaatgag gaagtgattt
     1681 ttgacaaaaa cgttac
//
LOCUS       KCVB01000098            1084 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4515pri ultra-conserved element locus
            uce-829, sequence.
ACCESSION   KCVB01000098
VERSION     KCVB01000098.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4515pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1084
                     /note="ultra-conserved element locus uce-829"
BASE COUNT          337 a          230 c          207 g          310 t
ORIGIN      
        1 ttacagccga agagcagctc ctcctgggtc tcacatcccc tgacacagtg gaaaaccact
       61 gagctgaaaa atatgaggaa catgatggta cctacctgtt tgtgcatctc tatgttcagc
      121 ccgtaggaca tctcataata ctgaaacgaa agcaaagatt tagttgtgaa taatggagac
      181 aaaaaaggcc acatttacaa tcaagttgtg ctaacattta ctttacgtat gcagttactt
      241 taaaaatgta tagatgtggc ttatttttat ttctatactc aaaatcataa ttttaatgta
      301 actagcacac tgtatatgct cacaaatcaa tattttggtt gattactaca ctaattacat
      361 caaatgtttt ttttaacatc atgactgaca cctaaacaca aacctgcttt tttggagcaa
      421 gtgtgtgata aactatgatc aaacaaaaaa ggagtctgtg agcagctgac agggaagagc
      481 caataaatcg ataatttgtc ttttatagtg cgggcacaga gaagaaaggg gttagattta
      541 ttgatcacca ttctaaaccc tcagatctcc aggatgtcta tttacttagc gtaacgtgat
      601 tgctcaacac tcttgataat tcaagccagg tcagtctgcc tggctgaaat gtaatccgtg
      661 gagcccacaa gtggcagcca gggcagaaat ggctttccag tcagcgtcta tttgatgagg
      721 ccaatgcatt attcagtcca ttagcttctt cgaaaattac tctcaaacaa gagagccatt
      781 ctgtctgaga tcagagcccc agagccgtag cgtaacccga cacccagtgc tggctgtggg
      841 atacttccct acatgtttac ctaacctcct ccctgtcagg ctgacatgtt gttttcctgt
      901 gaaaagaatg tttaacttaa gcctgcatgt attggtttaa gtcatccccg gtgtgtttac
      961 ctgagacaac agagcacact acctcagaag aaccctctga ttgcatcatg tctgagctaa
     1021 attcctgcgt cataagcata cctatgtttt tctaaagggc tttaaaaaaa aaaaaaaaaa
     1081 aggc
//
LOCUS       KCVB01000099            1514 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4560pri ultra-conserved element locus
            uce-4, sequence.
ACCESSION   KCVB01000099
VERSION     KCVB01000099.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4560pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1514
                     /note="ultra-conserved element locus uce-4"
BASE COUNT          420 a          382 c          324 g          388 t
ORIGIN      
        1 gttgaaggct gagcctctta atgaatagcc tgcaagaaaa aatgatatca cttcaaaata
       61 gacaaatatc tcatcattga atgaaggctt ttgtttagga ggccagactc ttcctgtcca
      121 ccttttctcc cacattataa atgatcaggc ctgatttcaa acatcaacca cacctccaga
      181 gcatctcccc actccagtcc tctctgacat ttagattcac agtgaacata ttaaatatta
      241 gtgctgaaat gattttagga atatcaccaa aactgatgca gctctaatgc ttaagtgtac
      301 cgtaatctgt ttcacagctg aagtgctgtt ggtaaagact gtgccttcat taattagctg
      361 ataaccttta tagaaggcgt gcaggcaaac aaacaaaatg attggtttgc tattgttgta
      421 ccttatcctt ggcgaatcac ctattgtccc tttgaataat tgagaccagt gcgttttgcc
      481 tgttttattt tttccttggt cagcaactag ctactagttt tgtccccaag atcaattgcc
      541 ttggtaacac aaccagaggg agagagagtg agagaggggg agagagagag atagatagag
      601 agagaggttt gtgcccacaa tggcgactgt aggtatgcta aatacctctg gttccttatc
      661 cgggaactac ctcttactct gctattgggc cactgggtca cgtggtaaaa gtaactttac
      721 agggctgctc gcaagtagga gggctttatg gagcagaaaa acgacaaagc tagaaaaatt
      781 attttccact ccagaaatta atgatcatga gctcgtattt gatggagtct aactacattg
      841 atccgaaatt tcctccatgc gaggaatatt cgcaaaataa ctacatccct gagcatagtc
      901 cagaatatta cagccgcgca agggacactg gctaccagca tcaccatcag gagttatatc
      961 ctccgcgggc gagctaccaa gagcgccagt ataactgtgc aagcatccct gaacctgaca
     1021 cgccaagggg acatggaata ccccaatctg cgcacctgct gacagggaaa gggcaacctg
     1081 cttcatgtga aaccccacag ttgtccatgt cccccgccac cccaccggcc gccacctccg
     1141 cctgcaacca agccaccccg gagcatccaa acagcacagc ctcctccaag cagcccgtgg
     1201 tgtacccatg gatgaagaaa attcacgtca gcactggtag gcaacttgtg tgtttatgtt
     1261 ccctgcactc ctcatgctcc tctctccctt tccctccctt tatcagtccc tctgactccc
     1321 atctcgttgc cagcccctgc tccgatagca gggaagcctt tgaaatggca caattgaggc
     1381 ggatttacga ctcggcattg gtaattacac ccaccataaa ttttatagcc gaggtatact
     1441 cagggcagcc gtatcaccat gtggcttctg ctgttatgta ttgcgcgata gagagagggg
     1501 gagaaatgaa taag
//
LOCUS       KCVB01000100            1301 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4600pri ultra-conserved element locus
            uce-891, sequence.
ACCESSION   KCVB01000100
VERSION     KCVB01000100.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4600pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1301
                     /note="ultra-conserved element locus uce-891"
BASE COUNT          347 a          298 c          328 g          328 t
ORIGIN      
        1 gtgtgtgtgt gtgtgttcgt gtgtatgtgt gtgtgcacgc tggacaaact gcactggcag
       61 gatttttgtt ggagtcccca gggcagaaga cacatgagtc agcggccagt aggatttata
      121 gccaattgat taaattagca tttgttctgc tactggggca gcagcctgtc agtataaaca
      181 tacactgaaa cacagacaca cacacataca gactcccacg tgggcaaaca aacatgcacc
      241 tgtacaccga ctgcagttaa ggaacgtaga agaagagaaa caagacaacg aggaaaggaa
      301 aaaagtaccg agaagcagca gacaattagc aatgatgtca ataagtcaac atgattaaca
      361 tcaggatcac tgggatgtaa atacttgaag aaacacacac tgcactccca caagcctcct
      421 tcctaagcag ctccttcatg tgatacagca agtgagcaag ggggtcgcac acaccgggct
      481 tgctgacaag ctttttttta tgacattcat caaagccatc tgccattttc cgctcaatta
      541 atttgttatg ggaagtggga tcaattaagt gcgcactgta atggctttat atccttgttt
      601 cccttttata gcccaatatt ttctgcgctc cactctggca tgaaaaggcg aaatcgatat
      661 ccccagctat tttcccctcc tgtggactaa tttgtttttg ccactctctt cctggcatga
      721 taaactggct gtaatgaggg gttagggcga ggggacggac taaacataaa cttgcactcg
      781 agccaacaag cgaactgggc acgctctttt ccagtgcggc cgctggatcc aagaagctac
      841 agactctaat ctgtcgagat ctcatttgga tgtcaggagc ggcgttgttt cccacactta
      901 actacagacg tagtagtatc aagagggatt gaccttcgcc ctgttatcat ccgacacaca
      961 ggtcagccgc agagacagtg agtggcttag aagacggcga tgataaggac aatgggtggc
     1021 tcattcggga cagttagcct gacagtgaca ggtggaggcg cgttggctcc ttgcaccatt
     1081 agtacgagtg tcacgagccg tcgtgtactc tcctggctca cctcgacccc tgcgatgtat
     1141 tgatcccttc cccattaagc ctgctgatgg gcttgatgat atgatgaaga attgggggag
     1201 tgatgtgggc gtgtttaagt gtgtgcatgt gcatgacttc agagcaggtt gaggggccat
     1261 cattactcct ggagcaggcc tgatgacatt gccatattga t
//
LOCUS       KCVB01000101            1397 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4644pri ultra-conserved element locus
            uce-446, sequence.
ACCESSION   KCVB01000101
VERSION     KCVB01000101.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4644pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1397
                     /note="ultra-conserved element locus uce-446"
BASE COUNT          493 a          240 c          282 g          382 t
ORIGIN      
        1 agtgacacaa aatcaaaatt cgaaatgggc ctgtgtgctt tttctgatgt taagtgcaac
       61 ggtgcactta acatcatgga gaaaccccca tgcaggccat gtaaatcaat ctgagttata
      121 ttgctataaa ctttatgcaa tataccctca gctgtcattg cttctataac gcacatttac
      181 atactgtcta cacgaacatg cacggatagt agcatgtaca cacacatgca gtacacgcac
      241 aaactccgtc acacacactt gcatataaga aatctattga gtgtatttct cctcgccaag
      301 gatttttttt tgcctgagtc aaagataata caattccttt ttttcttatt tccttacaat
      361 acatctttca gttctgcctt ttgcgtgcat gaaattcatt ttctaatgga aatatttgtt
      421 ttgtaaatca aatatgtatt atgcacagaa tgcaaagcac aaattaaatt atgttaatga
      481 aatgaaagag atatgaaatg catatttcat ggacaaaaca taaccctgcc gaacagccat
      541 ttcttgataa aatctttaaa cacaatctca ttcggagaaa gacatattgc catggcagat
      601 agcacctcat taaaatggtc tcctttaata tcagtgctta gtatatatgg gattgtaaat
      661 aaccactggg gaagaggaac caataatgaa atactgtctt taatagcttg ccattgatgt
      721 cagcataaaa agtctactag aggaatattg aaaattgggt gtgcacacac atatagtcaa
      781 aagctgtaaa tgattgcata tttaaataga gctgactaag gaaaagatga tatagatatt
      841 agtaataggc agaaaattat gctgacctta cataattcca caaaaaaagc ccagactgtt
      901 ttaaatttta tattggttac acttaatgaa taatagattc tgtagctgtt gttgtgcagt
      961 ccatgatgtt aataaaagcc taagaatagg atccctgctc tgcccggccg actagcaaat
     1021 gaactagact ggcttttcag cagaaataaa tacagcaata aatacaaaag gagggggacg
     1081 ggggtggcgg ggagttgggg ggggcaaaga caaccagaga aaacagggga acgatagctg
     1141 gatagagaga aagaggaaag atacagtaat agatagagga ggaaaagtgt aaatagacag
     1201 agggaaatat gaagaacaag gcaggaaaga acaacagcag aaagctgtgt ggacttgact
     1261 gcagcatggt ggaggttaga cgaggcttgt gactggacga cctgactcaa acgcattcac
     1321 cagtacatgc agcacatatt tccttagagt ttgactatgt gttttttaat gcacacaatc
     1381 atttcaaaag aagagtt
//
LOCUS       KCVB01000102            1024 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4685pri ultra-conserved element locus
            uce-93, sequence.
ACCESSION   KCVB01000102
VERSION     KCVB01000102.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4685pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1024
                     /note="ultra-conserved element locus uce-93"
BASE COUNT          313 a          167 c          215 g          329 t
ORIGIN      
        1 caaatagaaa tgcagagatg agtcatccac tgagctactg cagtagacgc gcacgcgcat
       61 acacaaacac acacacatac acacacacac tcacacacat tggattgatc ccagcgcctg
      121 ccatctgcgt gtgtttgtat gttggcctat tgtaccaggg aatgtgttat aataatgtgt
      181 gaaagtgaat gaatgggtgt tgaaatgagt cttttgatat ttggaaagta cagaaggtca
      241 ggctgttaca tgctgtcagg gccaacgttg tttctttgaa gaaagtttag accgaagttt
      301 gtactcaaag tgcaatttgg actgaaatca ctgagagctt tgaaagccaa atgttttttt
      361 ttttctctaa gtttattttg atatgagttg tacgtgattt gattcaaaca gaaaggcaga
      421 aaaagaccct tacctataca gtacagtcgt ttttgttggc tggatgctgt tgtcacttct
      481 ttaaaaaaac acttgctttg agagtattgg caaaaggaaa cttgacatat tccagtatgg
      541 ttttgttgca tttctgaagc atcaaatatt aatctcaaga aaggccttac tcttatggaa
      601 aaaaataaaa aagttgtgac tgtagacatt gttagaacag ttaagatatg ctaaacactg
      661 agctgcttca agggagttgc ccacagattt tttactgatt tcaagatttt tattgaacta
      721 gtttagtcat ttctttgggt ccctatcaat agaaaacaat cattatggtg taaaggcttt
      781 gaaagcatcc acagttaaat gcaatttcat ttaaatgaaa gtagaaacct gaagcttcaa
      841 ggttgataac atcttaagat ttagtccttt atgcagtgac cttgttctaa tgaaactggg
      901 gtcatactga gttcctgcat tggctgtttg ctgtcttgag tttgcaggag tggtatgtaa
      961 tagctgtgtc ctccggttta tgtcatgaga ataaaataca tttgctatgg tttaaaggtg
     1021 acaa
//
LOCUS       KCVB01000103            1001 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4728pri ultra-conserved element locus
            uce-1238, sequence.
ACCESSION   KCVB01000103
VERSION     KCVB01000103.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4728pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1001
                     /note="ultra-conserved element locus uce-1238"
BASE COUNT          249 a          224 c          233 g          295 t
ORIGIN      
        1 aaaaaggaat aactgctgcc gctcttgcac aaacttttag ttgcagtgtg tgtaaatgat
       61 ggggcgtgtg cgaggcagct gatttacagg agcgttgacc cctcatcaaa tagaattcta
      121 ttcttttggg ggtccctggt agaattaggc ctgtccttca ctctctgcac gctaaaacat
      181 cacaggcccc aaatgaaaaa gcacgagttt gtgtgtgggg atgcaacatt tgtagtgtta
      241 actgagtgtg tgtatgtgtt taggtttggg ggttttagta cagcagaggg agggaggtaa
      301 tgggagggga aaaagaattg taagaagaac atccccggtc tcacagcaac ttcaaaagct
      361 ccccttcccc aggatattaa gcgactgtga tgaaaaattt agcaagcgtt aaataagccg
      421 ctttgaagtg atctgttttg gctcagcagt ctccaaattt ccgtagcctc tctgcaatag
      481 ccatcatact atagaggggg gaagaggaac agtgtgttaa gtgcaggagt tgtgggggac
      541 ctggcaggtt ggcagaggac gtgccaacag tgatcggtcc agccagcaac atgaaggcac
      601 caccgctgtt cttgtcccct tttgtctttc tctgactctt tttctcctca tttcacctct
      661 ccttccctcc ctccctcgct gacggatctt ctgcagagcc ccatgagtct ctggcagaca
      721 gatgttcctc atatgttcac tctgaaaagt gatttccccc cccccttcac ttacacgtgg
      781 gtgtaacaca aatgtaacac tgtggtgata ccaggaagtc agaggttact ctcgggtctt
      841 gactcagtta ttttttttgt tcttaatgac tttctgcatg ggacatgtgt ttgtaaaaag
      901 atacatcttc ttaacacaaa tcttcccact gtgttttttt tttcgtcact ggattgtatg
      961 acttgcacaa ttttccctcc aaaaaaatca gagactatta t
//
LOCUS       KCVB01000104            1635 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4773pri ultra-conserved element locus
            uce-1181, sequence.
ACCESSION   KCVB01000104
VERSION     KCVB01000104.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4773pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1635
                     /note="ultra-conserved element locus uce-1181"
BASE COUNT          391 a          381 c          383 g          480 t
ORIGIN      
        1 gcctacctgg taaacatctc cacaagctcc ttgctttcaa aacacgtcaa ccagtgccta
       61 tgtactttag actggccagc caagcaggaa ttgagacttt atttgttttt tactttttcc
      121 tgtctctctt ccgagccacc gcacataacc tgtctggcat taatgatgtt gttaataaaa
      181 tgcggctggt ctgtgggtgg atttgcctgg caggcctggt gcctgtcctt cccagagata
      241 agcccactgc tggccttatc tacctcccac agccccaccc agcagtgccg tgcactggac
      301 acacaaccag tcaactcatc actggctaat taccacctct gactgcctta caaagcaggc
      361 ctggactcac atcgctgctg ctgctgctgc tgctaagact gactctcttc taccttgact
      421 gtgtctctct ccacttcttt gtctttttct acccatcccc ctttccccct aaacttctcc
      481 tgtttttctc tttcctttca atgtcttaat acatttcata tgtttctcct gactagtttt
      541 gtcctcttct gttaatatcc aggaagtact gagttttacg gcaatatttt ccaactgtca
      601 ctgagaaggg gatatgtgac atctacattg tgggtggtca ggtaaatcct gtgtctacct
      661 tccagtgtat gtctatgcat tttgaaaagg actaatgtca tttatgttac actttctcag
      721 taagggctta cgccatttgt gagccggagt cagttcagtc tcatagcttc agcagggcag
      781 ccgaagtcac ttgtgttaca gagggaggga gcggtttctt ttattcaatt gttttccact
      841 tccacgagcg tgtgccaagg gggaggaaag cgccggctta acacgctctg caattcaaca
      901 cggccagacg cagtcctatt aagatttaat gtgccattct tctgcttgtg ccgctgaaaa
      961 agacttgcag gctgaggaag agagtgaaca aagagcaggc agagccaggg ccagggagag
     1021 atgaagaagg caagagaaac cgagacaacc gctccacggc caggtctttg tcacacgact
     1081 gatgtcagta gtttcaaagg gatgctacat atcaacagtg tcgctgataa agtgttttca
     1141 ctcagtgcgt gtagctctag gtgagtacaa gagattatcg ctggcttgtc cacatgtcat
     1201 ccctgctatt gtggctacct acttaagaaa gaggtcaact ggatgaagtc gcagcgtgtg
     1261 tttagcctct tgggcgatga attatgtgtc gtctaaaatg caaagtgcaa gcacgcaggt
     1321 gctgagaggg agcatgtgtg tgcgcgtgcg cgttaatacg tgtctggata cgtgtgtgtg
     1381 tgtttgcatg cctaagcgga tgactgattg agcaagcaaa tacatgagag acagagtgtg
     1441 ttagatagag ggggatgtga atatgagggt tgcattgctg gccattgtcg tttctgcctt
     1501 gaacacacaa atgccagcat caaacatggg ctcttttatt cttattttga ttgttttttt
     1561 ctgcagaaaa tgcagtccta gtctgacaaa gaccaataaa tactttatac agtgcataat
     1621 gtgtgctgtg tgaag
//
LOCUS       KCVB01000105            1800 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4801pri ultra-conserved element locus
            uce-663, sequence.
ACCESSION   KCVB01000105
VERSION     KCVB01000105.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4801pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1800
                     /note="ultra-conserved element locus uce-663"
BASE COUNT          550 a          305 c          376 g          569 t
ORIGIN      
        1 ggacattatc aaacattaac taattgttat aactaaacaa tattccagcg taaatcaaat
       61 aatttttatt ctgaattaga aaatgatcac ctggaaactt tttgcaggcc ataagttcgt
      121 tattactggt tggcactttg tggctgcagt ggaattaaag aggttaacac cagggacaac
      181 atggtcttaa attctaaaac aactgacata tactgttttg tctggtgctg tatacaaatg
      241 tgcaagcttg tagacttgtt atatcctgac ctacaataca gcataacctg ataaagcttg
      301 catcactggt ttcataaagc actggcatct ggcctttggt caagaaagag cttgtcataa
      361 agtatccata gactgctgta cataaagggc attaggggaa agtagcctgt acatagcagg
      421 cttttattag gttactatag taatgtaaat ggattaaaac cactgctcag ctctagtgac
      481 tcccattgag tgagctaaat gattcactac tggattttag cccccctcta atcgaataca
      541 gtgtacactg tgtatgcgat gcaaactgtg atcagtgtca tttataactg gaatgtctga
      601 cttcgctctg gagatattac tttgacagaa ggatggagga ggaggagagg aagaaggcat
      661 aattaggaat gtgcagatgg gtaaaaaaaa aagaatcagc cacaacctgc tgtgttctcc
      721 ttgtgcatgt tgtgtatttt cttgcgcgtt gccaggttct gcgtactgta tgcattttgt
      781 tgtttcctcc cgtccaggtc gccagccatg gggaacctca ttaaggtcct tggcaaggat
      841 ttagagaact gtccacattt tttcctggac tttgaaagta agtgagcacc ggggtgtgtg
      901 cgcgtgtctg tgtgcttgaa atgtgagagc acatgtgtgc agcggaggtg ttcagtggga
      961 ggtcgcgagg tggggtcagg ggaggtatat ttggatgcga gctgctgagt tgttgatggt
     1021 gtgatggagt ggtgttgtgg gtgtggttta tcattatcag acgctctcta gtcccactct
     1081 cagtactttc tgtggccctt ctatccattg ataagtataa atacggggtg cgtaggtata
     1141 gataaaatgg tttgtaggta atcaggcaaa caagaggcag gataaatcaa aaccagtaaa
     1201 atttacagag aagagttcat ttatgctgtt tcctcttcag caacactgtc cttaaataat
     1261 tttctaagag gaaagttaca actgacttat atgtactata ctctgacttt gatacattat
     1321 atacagcttt gactccttac aacatgcaag tgggcacttt tataggaagt tacttataga
     1381 ttagatttat tacaatacta ttcaacaaaa tctcccaaaa aataaatagg aataaataaa
     1441 ctgagattga gtaaaaatta tttgaaacac aattacctta aaagtaaaaa tctgcaataa
     1501 agctgtacaa gagtactcta tttgatgatg acattgtctg cttttatgtc atggaaacta
     1561 accagttttt tttactttcg ttgctccagt tttcacctgc acctctgaca caaaaaaacc
     1621 ttacagagtt aacgttcgac cacaaatagg gcacttctgt gtaattttag aatatgtttt
     1681 gactataata cgtcatattt tcattatgtt actgttgaaa aaaataaaat ccatatataa
     1741 tttgagattt ttaccaaaaa atgaagaata acttgtagta tttccatttt ttttacatag
//
LOCUS       KCVB01000106             963 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4845pri ultra-conserved element locus
            uce-626, sequence.
ACCESSION   KCVB01000106
VERSION     KCVB01000106.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 963)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4845pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>963
                     /note="ultra-conserved element locus uce-626"
BASE COUNT          217 a          254 c          204 g          288 t
ORIGIN      
        1 tctttgtttc ccctttcctc tttcagcatt gttggatctc gctgtgtatg tcgatgagag
       61 cagggctcct ctgttatgtg gggatgaaaa gcctgctata tccccgagcc tgagggtgag
      121 gataattccc cttaagacct cccaaattct ggctccttgt tcagccaccc ttgggctcac
      181 tacagggggt aactactata cattctgttt ccttttctcc catcagacag cacagcgccc
      241 tgggagagaa cacacacatg ctgtacataa tacattgcta cgtttcgatc actatgtccc
      301 tctgatactc agcagctgtg ggaggaaaat gcaggctatc tccagactga tgggaaatgc
      361 attggctgac catgcagcac tacgaccgta cagaatgtac agtaaaactg agctcagtag
      421 gaacgatgcc acatgccgac acctcatgga gtcagtctga gcaaacagag atctccagtc
      481 ctgatgattc cgatttttgt tttttatttt ttgctctttt tttttgctgg ctaactagct
      541 gaactcctct gtggtgagct gtacctcatc agcacgcatg atgtctaccc aggtcgatag
      601 ctttgtgccc acaagcatgg ttgaagtcaa tggaattttt attaggaaac aataatggga
      661 gggagggggg ggcgggtatg aggtggagaa agagagaaag cgaggtgtag gtagtacatt
      721 gatttaccct tcatagttcg ttgcactaca tttacccagc agagtgctca aattagtgcc
      781 ctccagctct ctcccaatta gtttccacag agacgaggtg taaagccagt cactggcctc
      841 attacctctc cactctcctc ctctcactcc ctccccacct ctctctgttt tttagttttc
      901 ccctctctat ccacttcagc ctctctctca tctcctccac tttttttttt tccatttcag
      961 tcc
//
LOCUS       KCVB01000107            1700 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4928pri ultra-conserved element locus
            uce-1280, sequence.
ACCESSION   KCVB01000107
VERSION     KCVB01000107.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4928pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1700
                     /note="ultra-conserved element locus uce-1280"
BASE COUNT          386 a          414 c          330 g          570 t
ORIGIN      
        1 gtcactgccc ccctgtcctc atctttcaag agctcttttc aattaattgg ctctttgtcc
       61 tgtgccagac tatgcaaatt tattacaccc cagcagtatt ctgctgctac tggggaaaaa
      121 gtgtttacaa ccttcattgt tttgcagcac ctcccctata gagggctggt atgatgtttt
      181 aatcaaaaag ctaactggcg ccatgcataa tttactctcc tcattaacgc tgtggccact
      241 gggcggtcat ctcagggtgg gacaatgtct tctgtctcag atagtgacga agagagaggg
      301 gtcgccgggg ggttagctgg aggagatctg gatgcaaagt cccagatgtg caactgatag
      361 atggatagtt tttaatcaga gctctttgaa gtcaaattta ggtgttagct tcttaaaaat
      421 gaagaaatgc ttgtcgaacc taatgttttt tctcccctgc ggttgttttt gcatttagaa
      481 acaaccaaat tttcttgttt tcaccttttt taaactgaat cctaaatgaa gctcttatta
      541 ttttattaga agtgaatttg gctgcagcaa tagatctccc agctactcat gactagtctc
      601 ataagtcctg gtcagcagag cagaataagt taattattta ccggtcacac gcacacacat
      661 acacacacac acacagctgt ggaggtgggg ggaggtggag gggttgtcag tagtaggggc
      721 ccattgtgcg agccgccagc gtggagctgt atgagctgtg atggagggct ctggaccctt
      781 gctgttgtcc tgcctttctc tgctgatttc atgcctcttt tatcattcac tttcacacaa
      841 aacaaacaac accgaggatg tctgctatgg gaactagtgt ctctctcttt ctgaatctct
      901 ctgttcaact tctccgtttt ccaccttcct tcctcctctc tttccttttc catccttctt
      961 ttaccccgac atttaaacct tttttttaac tgccttttct ttctttttgt tttctccttt
     1021 taccttttct tctgggcctg ccatacttgc cttttctctt ttcttttcac cttcttcctg
     1081 ctgttggatg actttccagt aaattgctgc tcttgttccc agtcaacatc ctgttactgc
     1141 atcctgtatg gcatcaacag tctgctgtgt tgctgactgg tcagctaata gtgaattttc
     1201 cccacgtgca cggattcagt ttctcctctc cagttgatcc cctccacttc ctctgagttg
     1261 agctgaaagt catggatgtg taatcccggt ctccctgtca agctaagcac ctttctccac
     1321 ttcctgctct ccttctcttc tctcctctct cttttttcat ttgcaaatgg gcctacaatc
     1381 tgtttaccct ggccagggaa gagtgcctca gcccctccgc aaacatttgc actcacactt
     1441 aaactcctgg ctaccagggc gctcgtcccc aacgtcatgt gaatccaaag acgaccctgt
     1501 cggttcacat gagcatggaa atgtcatgca gcaacaaatg cagcaaaaag ttttattata
     1561 agtttgctaa tatgttggtc atagctgtgt ggtcagataa gttagtgagc aaaggagagc
     1621 tacaactatt agcaattatt ttccttattt taatctgtta ttttctttta gataaatatg
     1681 taaatgtaat gaaaaatgat
//
LOCUS       KCVB01000108            1462 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 4976pri ultra-conserved element locus
            uce-1061, sequence.
ACCESSION   KCVB01000108
VERSION     KCVB01000108.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="4976pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1462
                     /note="ultra-conserved element locus uce-1061"
BASE COUNT          388 a          340 c          305 g          429 t
ORIGIN      
        1 ctcacgccag cctcgacccc ttgcatgata atgaggggga tgtttgtgag aggaaaagac
       61 tgacccaaag tgtgtcagtg aaatgctagc ctcctaacac cacaccatca ttactgtcac
      121 ctcactttac taccactcca ttgatgattc agcgttctac ttttgggaaa ttcacacctt
      181 caaaatttac tgtgaagcta aacttacaat acatatttga tttagtgtaa aatatattta
      241 catgttgcgg cattaggtat gacaatttaa aacagtgtgg cctgtgtttt gacttcctac
      301 acacatacag agtcgctagg ctaatttctc ctcagcatgg gtgttggaag tgagaacagc
      361 acttgcagcc aaacatgtct gtcaatcatt tattgggatt ttgacatcct gcctcgaaaa
      421 tgttgccctg ctatcacagt atcaacactc tgcatccttt tgcacgtggt ttatcattct
      481 cacaatgcca gagcactgag gaattaatat tcatcgctgg taatttatgg attcatttat
      541 cacccacatc ttatttgttt ctgttaatcg accctggggg cctgactatg gccttggctg
      601 gccaccagtg ctggagcttt attcctcttc acacaggcct agtgttaacg ccgctggcca
      661 agttcacaga tttgactgac tgtggactta atgccgcttg ccttgttttc agcacttgtc
      721 cctccagatt tgccaccctg gctgcacaat ttcctccaat tctaattatt catcagctcg
      781 ttatgcctgt gcctgggcgc ctcccctaat tagaggcgac gcagggtttg gtcccaggct
      841 agccccgagt gagggaacca gttcagaacc agtccgcggc gtgcgctcat ggaggactgg
      901 gtgactgcca gcctctccaa gtgcacaggg gagaggaggg cctgtcttga cagtttgatg
      961 tgacgtgcct tcacacagtc tctctcatac acactcacac acacacacaa acactaatca
     1021 ttctgctatg tcccatatct attagcataa cctctagata tttagaagaa ccctggaaca
     1081 atggaaattc tttgtcttag tggtgttatg tattgccact ctagtgtgat ttttttgtgt
     1141 gtgtgtgtgt ggatttgtta ttatgccgtg aagctgagat ggagatgcag ggctgctgag
     1201 aacagtgtca gtgggcaggg tagaagagcg tgaaatatta aatcattgtt ccctaattaa
     1261 catcaggcct tagacacaaa atagcccctg gagctgagaa caattgaaat tttccattat
     1321 tattaaaatg aacaatggaa gatggaaaac caacaatggc ctcttgctca cataacagca
     1381 acatatcctt tcataactgt caataccaat tgtatttgta tgaacacgag caaccatagt
     1441 tacaatatgt aaaagtcatt ga
//
LOCUS       KCVB01000109            1673 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5017pri ultra-conserved element locus
            uce-1024, sequence.
ACCESSION   KCVB01000109
VERSION     KCVB01000109.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5017pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1673
                     /note="ultra-conserved element locus uce-1024"
BASE COUNT          510 a          352 c          391 g          420 t
ORIGIN      
        1 tacagagcac ctctgaggag gtgctggaat cattacaagg acttttatgt tcctacatcc
       61 gtcctataat gcctggaata gagctgcaat aataggacaa gactgacaga gaggagagga
      121 agacagagga gaaggaaaga agtaaaagga gggaaattta agcacaataa gaggggataa
      181 ttaaaaataa gaacaacaag aacaagaggg gggaaaaatg aaacattttg catgataaag
      241 agatgcatta caaaagctcc acagcaggcc ttaagcagct ccagcaagcc tcctttttat
      301 tgtaaccctg caattaaatg tatgggcacc atgtgggtat gcatacatcc tgtaccacaa
      361 actcctccat gatgactaat gaagttgaca cagggctttc aactattcat gacttgaggt
      421 ctgaattatt tgttgagttg atgtaggcag gcaaactgtg tttccagcga tcaagcccag
      481 aaaaaaaaaa gaaagctgtc cccctctgtg ggaatcttct gactaggcgt gcctttgatt
      541 tcagaaacac tgtctctgtt tcctacatca gcccctcgca gtttacagca actccttggg
      601 gtggacgctc catgcagctg tgggcatgtg agagagagat ggagagacag aggaaaagag
      661 gaggaagagg ctaagctaaa gctcattatg cttcttgctt acaggcgttg tgtggatatt
      721 ctattagcca tatctaaagt gtaattgtac cattgggggg ggagcagtgt tgtcggggtg
      781 ccaagctttc tctcagcgac tagcgaagtg tggtaatctc acacaccatc tcattacagt
      841 ttaaaccccc agcagctgct cgcattagac ttttattaca cgctgccgct cacagctgtg
      901 ctggcatagc cctggacagg atggtgagag acgggaacaa agacagacag acacagggat
      961 tccagcacaa catttggctc ccatcaaaaa agtggaaata tcgaagcaga gggtagtaat
     1021 gaccttaaca gcctctcagt gtgataactc agtgcctccc cccccctcca ccccagaccc
     1081 taaactgagg ccaaggacag tcgcacagat ggaccgagtc aaacccagtc agattgggat
     1141 tcaatcaaaa ctgccagagc cgtggtcatg caaaagcttc acgccgggac agctttcata
     1201 agcatttgtc tcgttgtgca accagtatgg ggcggtggca ctctgttatc tcaacagtca
     1261 catctgtttt aagcctctgt aaatgttgtg agtggtgcat gagtgtctct ccatgcacac
     1321 aggcagagtg cacaacaact tgaaccactt tgaaacatat tcaatatatt tgatcatgtc
     1381 ttataagcag cgctgcagcc gggatgttag aaaaactgag gtcaagagtc aaaggagtcc
     1441 aactcagccc aagaaggttt tgactcctga attaatatta aattattttg tattattttg
     1501 tgttttatat ttatttagtg aactgtccaa ttatattatc tgtaaatttt cccaacatga
     1561 agctctagat cagggctgct caaagtcagg acctctgtcc acatctggac tgaacgtgtt
     1621 gagacagtta atgaaaaagt tattcagaat gaaaaatttt tgaaggagcg ttt
//
LOCUS       KCVB01000110            1444 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5056pri ultra-conserved element locus
            uce-855, sequence.
ACCESSION   KCVB01000110
VERSION     KCVB01000110.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5056pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1444
                     /note="ultra-conserved element locus uce-855"
BASE COUNT          436 a          218 c          259 g          531 t
ORIGIN      
        1 gatgtaaaaa aaaaacaaaa ggccttgtca cactggattt ggtaattttg acgcacaggt
       61 tacggtaatt gattgcttgc atatggcagg aatcataatc aatctttctc tgtctcgttt
      121 tttctctcct ctctgccatg atcctttgtt tcttttctcc agtgtatcag ggttaggacc
      181 aagacaagct gctgatggga agctccacat gggctgcaag cccatttagt ctgccttctc
      241 tcatttctgc ctattgtcaa attgaatgta cttctctgag ttcatagttt ttttttcctt
      301 tttcttcttt ttgtttctaa aatgtgccac tgttgagctg cctcgtgtta atggtgctaa
      361 gaaagctgac aattgttttg tttttctttc ttttcttttg ttttcccaga gttaacaaaa
      421 aaagaaatgc aaaatagatt aggtacctaa ccctctttat gaatgtatat tataatgtta
      481 tgtttgatgt attcacttca tgattctttg acattgattg gaattacttt tgctcttttc
      541 attaaaagat taccgagctt tatatatact gtctagagtt gttaccagaa ggaatcttaa
      601 tgaactcttg acactacaaa atcttgtata ttttttgtgt gccaatttgt aacatatttt
      661 gtaagtgtat atttttctta gaaagtgctg tgaagtattt gtgtgtgtga gtaacctttt
      721 atgcgcctct atgggcagtg gaaaggatat acaatgacaa gtttctggtt ttaaaaacca
      781 aaatatgaaa gtatcaacag aaaactagaa atatttacat tttgttggga gttttgtaat
      841 aagaccatga tcttgttgtg agagtgttgt gttactgtgc aaaacacaaa taagcaaaga
      901 aaaaaaaact gaaaacttgg aaaaaaatat aatttgtgaa caatttgctg ttttatagat
      961 tttcatgtaa attatttgag tattattttg ttttttgttt tgttgtaact gttgcaaagg
     1021 ttttaaatat ttgtgatcaa gcctgtatgg tgataatgta atattggtaa cttgctgagt
     1081 tttgtaataa tgtttatgga gtaaatatac aaattaaaat aagattttga atgctgcttt
     1141 ctgaagagag ttgggttgtg ttttttaccg cccacccggc gctgtagcgt acactgtaaa
     1201 cagtaaacat taaatctccg cactactaac ttctctgtaa acaaatttag cccattcaaa
     1261 ataagaacta taaaaggtta gggcatgcag ttttgtgaaa acagccagca tgcacgctgc
     1321 cttaatatta caaaaaactt attgagatgt caggaatgaa gtgtaatgaa catatctgtt
     1381 aggcaaaccc cttcatcttt gtggtactac tacaataata gtaatagcaa agaatatata
     1441 actg
//
LOCUS       KCVB01000111             727 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5116pri ultra-conserved element locus
            uce-810, sequence.
ACCESSION   KCVB01000111
VERSION     KCVB01000111.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 727)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5116pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>727
                     /note="ultra-conserved element locus uce-810"
BASE COUNT          225 a          171 c          123 g          208 t
ORIGIN      
        1 aaactaagag gaatactccc acggccgcaa aataacatat ggctgccttc actcaaaaat
       61 gcatcccatg cacttacagg gctttaggtt tacatcatat ataaacctga tacacagatt
      121 tcatcctgga atctgcagcc tgatagccct ctgtctttgt gatgctttct cagtgataaa
      181 gcaaaggttt agtgcatcct atcctggacc ggtgagtaat tggcatttta aattaccata
      241 caaaccatgt atgcaaagga agtaaatcag atacaactgg ccttttagtg catctgtggt
      301 ctcgcgttta caaatatgcc tattaataat acaattcccg gaggagatta caagcaccct
      361 ggatgccgaa tgtcctctga attgatgctt gagctgggaa tggtgttttt tttacacaca
      421 ctatttccac caagagggat actgcatctt taagagccct acaaaaaaaa atgaaaccta
      481 ttcctttgta tctccatgct cttatttcaa tactcctctc tccttgttcc ccctccacac
      541 atcattaaac atattattat aacatgactg tcagcctgcg tctctcttcg ctccattctt
      601 gctctgccag gtaatcaatt tgtcgtcact ctctgtaacc ccagtaaagt catcaagtga
      661 aatgagttac agctgcaaag agccttcaag aatacaggag gagaaaaaaa aaaaaaaaaa
      721 tgaacgc
//
LOCUS       KCVB01000112            1799 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5157pri ultra-conserved element locus
            uce-57, sequence.
ACCESSION   KCVB01000112
VERSION     KCVB01000112.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5157pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1799
                     /note="ultra-conserved element locus uce-57"
BASE COUNT          565 a          328 c          319 g          587 t
ORIGIN      
        1 acacttgcat aatgacatat ctagtctaga ggttttcaat cacacaatat atacacactc
       61 cttaactaat ttgagccggt gttccaattt aatgaagtta ttcccctctg aataatgaaa
      121 tggcagtgca gtgctcttca taccagtaag ataatacata aaattgacag ttataaagtt
      181 ggattgttga accgacaccc ctaacccatg gcggtgagtg gaatatgtgt gtgtgtgcat
      241 gcatgtgcgt ttagtcaaaa gcaagctaaa gctggaagct gtactcacct gtgctactgt
      301 tacatgtttt cagggtttgc tcctcgcctt cagaatccat ctgtaaataa aacatgcgat
      361 gtccagatga gccgctaaac agaaagactc tgtttcattt tattttaatt catttctttt
      421 atttgaaatg ggaaaaggct taaacacttt gtagattatt ggtgattttt ttttcagtca
      481 acccagagaa cagaaagcct taaaatcaat ttgcgtgcac aacaacatca caaagagctc
      541 ttaaaacatc agtaatatgc aaacacagaa agtacaatgt ttggggaaaa aaagtttcaa
      601 aggtgagtaa aaatgtaatg acactaaata atgacattaa agtacggaca gaacctcact
      661 gtgtgtgttt agacaaaaat agttgctaaa atccatccac tctcgctggc tgttgcttgt
      721 gggccccaaa taaaacctat taaacataat tattccaggc tgatggtggc gtatagtggt
      781 cacaggccag tgaaatctgt gagtcatagg atatgctgtt acttttccct ggtaatggga
      841 aacatgactc ctctctctcg tttgctgttg tgattgaagg cactgacctg tcatacattt
      901 ataatagggg agcaggtgtg gcagtgatgg accttaattt cctttaataa accttttatg
      961 tgccacactc ttaatgccac gccgggccct cagaggtcct gctcataata ggttcattac
     1021 ttttgattac tacagcctat tgagacaaac aactcccaaa cactatagat tacattctgt
     1081 aaaaacgaat gtacctcatt agtttgtaac actttttcac acagacatgc ttcaatattt
     1141 tgtataaatt tgttgttttg aaggcaaaat gttgctccta cgttctgcaa cctcagactg
     1201 taacatggac cttaatcaaa gttcaaatga tgttatcagt gtatcagaaa aacaccaatg
     1261 actcacatgc atttattacc agggtggttt tttttgtttg ttttatttgg catttgagaa
     1321 atgcatttat catgtaaatg tttttttaaa aggagtattt tatcaataaa cttgttatat
     1381 ttaaaaaata tatttttaaa tcttttttgt atttttattt ttctggatga aataattaaa
     1441 atctgtttat agtgacagca gcatgctatg ttttaattta aaagtgaaac cttaatgcta
     1501 caggaatgac ctaataatga gtaaagggtc caaaacctca atttatagta ggtcttttgc
     1561 ctgttcaagg aatgaaacat gaagactctg actatagctt aacatacagc gtttcaccat
     1621 gagcttcgta tcctgtttca cattttacat aagtctgaat cctttctttt gtttccactg
     1681 ctttttaaag tgcgtcacat gtccctttct gatacaatta tgacccaacg gattgtgaca
     1741 aagcagaggc tactgtatat ctaattcaca taacaaggag tgacaagtaa cgagacaac
//
LOCUS       KCVB01000113            1330 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5194pri ultra-conserved element locus
            uce-1325, sequence.
ACCESSION   KCVB01000113
VERSION     KCVB01000113.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5194pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1330
                     /note="ultra-conserved element locus uce-1325"
BASE COUNT          412 a          244 c          328 g          346 t
ORIGIN      
        1 catcaggtac tgttggctat tgagcatcag ccaatcagtt cctgatgcag tatcatccac
       61 atcagaaagc tcttccttca ccccagcgtc agtgcagggg caacaaaaag taagtagttt
      121 acagcatatt ttggtccttc tgtaacagca gtgagaggag taaaacaaaa aatagtctaa
      181 actcataaaa acaagccatt actcttacat tattcttttc atcctgcaat aatacaatag
      241 ctcatactgt acagtacaca tcattgtaag tcaagatggc agcagaagca tctggaaagt
      301 gaattcatgc aaattcaaaa tgggcatgca ttatgaaaga gctgcaagag atgaggctgc
      361 acttcaaaag actgtcgaaa agaaattttt tagaggtggg gaagggggat gtgagaggag
      421 gaagagcaga cagagagagt gtctgtgaat cgcgctcgtg taattgcaat gacatcttta
      481 ttgagagccc tccgtctctt tcctgtgtgc tccaaagggc gtcactgacg ccggtcgatg
      541 gggagagcag ccgaggcgct gtgtttagca taatcccaga ggccattgtg agagcagcta
      601 gtgaacgact cacagttgcc agctgagatt tcaccaaaac acagcaaaac acgacgagaa
      661 ggggatgaga cgggaaaacc agaggtggag gaggggaaga gtggggaaag agttgaggct
      721 agagacagga ggatcagtgg cagtaatgaa gaaaaaatgc agatgtgtca gatattatat
      781 tacatggaat ctgaagagta atattttgta tataaaggtc ttttgctgag ttgttggggg
      841 ctggcaggtt ggttgtaaca tcagggtgga ggtggttatc tctggtgtgt ttgtgtgtgc
      901 agcttgcaca gcatcaaaga ggcagagtat aaagtgtgaa acatgttttc ttccaggtgg
      961 actgcacctg ttggccctta ttcaggactg caattcaata atcttccccg tttcttctct
     1021 taaacaatca cttactgttt tgacttgtaa cagtgaggca taaaaaagtg tcccatggca
     1081 acatctcttc tattgtgcaa ccatcattac cttaagctag agtgtgaaat cctctttgat
     1141 caccgtttca ggtaggcaga ctgcagccaa cctttggtaa acaatttggt ctcctcctaa
     1201 cacgttccag gaaacattaa gatgaaaatt aaatgtattg ctgagacaac agaaacaatt
     1261 cttcaaaggt ttttctatag attgtgaaac agagactgga gatgaaataa agacacagtt
     1321 taagggttta
//
LOCUS       KCVB01000114            1259 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5231pri ultra-conserved element locus
            uce-783, sequence.
ACCESSION   KCVB01000114
VERSION     KCVB01000114.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5231pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1259
                     /note="ultra-conserved element locus uce-783"
BASE COUNT          307 a          263 c          309 g          380 t
ORIGIN      
        1 gcaggatgtt tttttcttta ccagatgtta ttgttgtcat aatagctttc agcctttgcc
       61 accattcctc ttatgctact atttcgacct ttgctcgttt ctgcttgttg ttatgttttt
      121 cttggtatag tcttttctgt ccctgtggac atttttatct taaacaccgc tgccattata
      181 gctgctccct cagaggcggc attacttaca gaagcgatgc tccacggcct tatctccctc
      241 tcctcttgaa aataaacttg gtctggccag acagggagct gtccagtgct gaaagtggta
      301 acacaggtag caacatcact gaagctttta gaggtcagcc agagtcagca gaagacaggg
      361 tgcaacagta acacgatgtt gagaggcaga gacacactga gagcagctgg ccaggaaggg
      421 aggtagaggt ggtggggcac gaggtgaagc cagaaacgga gagaaatgcg agggtagagg
      481 gatggaggac gaaatgggga atagagcgac gagggtatca ttctcagtta cactgtttca
      541 catggtggag cccctcttta aaaggccaat tactctgtaa tagtcctagg ctgactaggt
      601 gatttctcac tgtgctgagt ggaatggcag tgcaggagtg gagtcagtcc agtggagaga
      661 cccaatcatc attgcactgg agtgaaggga gtcaagggac acacacattt gcggctgtgc
      721 gtgcgtgtgc gctttttgtc ggcgtgcagc agcagcagca gtctgtgtgt gtaattgtct
      781 gtattccact gaattcatta ttgaatgtga ccttcaagca ccgcaagcat ccttcagtag
      841 aaaaggaatg tgtgtgtgtg tgtgtgtgtg cttacaggcc tgtgtgtgta gcatccctca
      901 gcagggagaa attatgtgta caatacctct ctgatgaagt tatatacaac aaatcggtga
      961 gacgatacca gaggctaggc tgaattcact ttcaaatcca attgaattgg aattcttctt
     1021 ctatatatgt ttcagactta gttttggtgt taaggtgatt taattgaaag taaatttaat
     1081 ttccaaaaag cttagcaacc gttaattgac agccatgata aatggtgttt ctgtcacctt
     1141 ttccccgctg aatcttgcct ctgcccacgc caaaatcccg tttatttgtc tctctgcctc
     1201 ttatttattc ttctaggccc ctttttcttt tcctgctgtt gttcatttcc aacatggct
//
LOCUS       KCVB01000115            1203 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5267pri ultra-conserved element locus
            uce-407, sequence.
ACCESSION   KCVB01000115
VERSION     KCVB01000115.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5267pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1203
                     /note="ultra-conserved element locus uce-407"
BASE COUNT          349 a          308 c          191 g          355 t
ORIGIN      
        1 atgtcacttt aatatgtgtc gttgaaatga taagataagt atgaaaaagt aaatttaaca
       61 atctttgatg agcagaaaaa taaaattata acattttatt ctgcagactg ggctgactcc
      121 aacgttaatt cacatgaact gtcaaaatcc aaaaatctaa ataagatcca tttatgtgtt
      181 taatttttta gtgcagaaag caggtgaata aatgtgacac acattaaaac aaagcggagt
      241 caaagggaaa aatctctctg acctctgtgg ctcataaagc actgaaatta aaaaatggtt
      301 gaatttctct cacgtctttt aattttatgc tgctttctct cttcttcatc ctctttccct
      361 tctctctata ttttgtatat atccctcttt ctttatctcc atccccctct cagtttcttc
      421 atccctcctc tatgtctctt ctttttctct ttgtcttttc atccccccat tctgtgtctt
      481 ccttctccct ctttgctctt catcgctttc tgtctcttct gtctccctct ctgagtaagt
      541 gagtttcatc tggggtgctt gccgggcgaa atcacctcag ggcgaacact ttattatctg
      601 tagtcaaagc gtcaccacaa ataccattct acacacacaa gtgtcacttt ccatttgtct
      661 gcactctggc tgcattagca gagcctagcc ggcccagagc ctcgtctcaa acgcgctccg
      721 gcagaagaaa actaacagcg tgggaaagca ctcgtcctgc attcctgccc cattaacacc
      781 actggcaccg ccacagaaga cctactgcct cactctggaa ttaaaaacac tgttatcagc
      841 actaaccctt gtgaagatct gaaacgacaa aaagataaaa agatatttag gcacgcttct
      901 tacatttcct ctcctttcat actgcttttt aacgcaagaa atcacagcac acatgaaagg
      961 aattcttttc tctaattcaa agtagatcac tgcacgtgtt aattactgat gatctgtcca
     1021 gcaaaaacaa caccagcggc tcgtttatgt cctcttggag ccacagagga atgcaggcca
     1081 gcagcagcag ccatgtcagg ccttttgtgg ggcacccgtg caccaaacaa acagaaactg
     1141 gggcaacatg ttaaactcac actacacact gcactccata aacaaaacag ccaaacaaaa
     1201 aga
//
LOCUS       KCVB01000116            1294 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5304pri ultra-conserved element locus
            uce-442, sequence.
ACCESSION   KCVB01000116
VERSION     KCVB01000116.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5304pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1294
                     /note="ultra-conserved element locus uce-442"
BASE COUNT          368 a          302 c          268 g          356 t
ORIGIN      
        1 aattactaat cttttcttct aagaaatgta ttaaattgtg atctctccta aattatttcc
       61 cccgaacagg aggaatcagg taaactgcag ctgcatggtc agttttgaca aaaatataat
      121 ctataaatcg cgtgtgaaat atttcatagc cttgtctgat catgaatgtt agatatagtg
      181 atatatactc acagattggc tccctattgc ggctcccttt cagcattttg agcttatttg
      241 gacttgtccc tctctggtgg ggcaaaatga tgcttaagaa gcataaatga gctggtcata
      301 caacttcatt gtgcctcttt gatgatactc ggtggccctt atgtctgttc aaagtgctct
      361 caaagtttct agaaagagtg gaattcccta ctgacgcaaa atagtcatat gtggtcagat
      421 cagctgatgt gcagaacata atgggttatt ctcacctgta aatgtagttg tcacaaagga
      481 cagaatcata aatatgtggc gtcgttttat tcatgtatac aacttaatgt aaaacaaact
      541 gtattttcgt ggaatatttc cctaaaatgt caatgactat tgtttgctgg tttaggagtt
      601 ttgctccgcc ccctgtgttt gtcgtaaacc tggctcctgg ctccaataaa cccgggctaa
      661 gagcgccctg ggctcgcctc cttctccaag gcgatcaata ggatctcagg cgcactacaa
      721 gcactctctt acgtagacat ccaccaagga gagagccgat acaacaacac ggttgatctg
      781 ctcaaacaca aaaatctacc tgctttctga atcatgtcgt tgagcccaaa gcacacaacg
      841 cctttttcag tgacagatat tttgagtcca atcgaggaga cctacaagaa gtttagtggc
      901 atggatggcg cggggaacct aaccactcca ctgggagcct accgacagcc tcaggtgtct
      961 cagaccggca tgcaacagca ctccatgggc cataacgcca ccgtggccac cacttaccac
     1021 atgccgcaca ctgtctccca gttctcccac agcgcgatgg ggggatactg caatggaagc
     1081 attggcaaca tgggagacct cccgtcgtac caggaaagca tgcggaatag tgcagcagca
     1141 acagggtggt acagcgccaa ccctgatcca agatactcca caagtaagtt ttttactcca
     1201 tttatacaca cttttctgat ttaatttcct cctaaatcca aaaattaaat tggcatttta
     1261 ggaatgagaa gggaatacgt tcaaggataa atat
//
LOCUS       KCVB01000117            1534 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5348pri ultra-conserved element locus
            uce-706, sequence.
ACCESSION   KCVB01000117
VERSION     KCVB01000117.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5348pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1534
                     /note="ultra-conserved element locus uce-706"
BASE COUNT          453 a          290 c          327 g          464 t
ORIGIN      
        1 cagggttgtt gtttttttgt cttgatcatc ttaaatatat ttctggaaac tggaaacaaa
       61 tactcttcct gttactcaca gcagttgtgg gattgagaga aatattgcta caacattttc
      121 acaaaatagt tatttgtttg gcacactatt aaaactttcc tttaatctta caataacttc
      181 taacatgtgg atgattacag ggagagcaat agatatgcac aggcaaatta tggtccaatc
      241 tgggacgctt agatttgaat atcagcatgc ttaactacaa agaccatgct agcatgaatc
      301 atataagggg aaataagagg aagaaagcat gcagctaaaa gttccacatc cttggtttca
      361 gtgggacttt attagtgtag agttatatat tatttgtgat tatgaggctg aatttaaaag
      421 gtttcatggt agaagttctg caaggtgcca agaaattaca ttctagggga atatgctctg
      481 atgctgtaac ttcatttaaa gaaatgaacc gtttatatta accaaaatga agacaaactg
      541 gtacagtggc tgctctttcc tgtgaaagtg atcacatctt tatggctccc gtcactgctc
      601 tttacagatc tgtttcccag aggtatttga tggggagagc caaagaggga ggaagtgtgg
      661 gtctgcagcc cccgtgttaa caatggctct cctcagaccc ccggcccagg cccagccagt
      721 catgaaacac catggagatg ttattgacct cgaggttaac tctcctcagg taagcctggg
      781 agataatgga caaagagcag ttcttttaca ggtggagtga cacggcatat ccgcgcagcg
      841 cagctcctct cgcattgaca gattttctaa ttcactgcag attcccataa aactgggtgg
      901 ccagggtgag ctctgcccaa cagagggccc attcatgctg ggtgggatag gggacccggc
      961 caaattaacc tggattaatt taaaagagta aagggtcctt acatccaaat aaaatgaaga
     1021 tcaataaatt aaatgtccag gtcaggatgt caagttgcag cgatgatcga ctgacataca
     1081 gaaagctacg cgctttggct gagaggagta ggggaaaaaa atgggcgaga gatccttaat
     1141 tactttattc caaggaagag aagtgcttcc atacgtctct ctttacatgc tagagttggg
     1201 ctcctataaa cacgctcatg tcagaaaaga atgctcatta ctcctctcat ttggccctgg
     1261 gattagttac tgctacagaa aagtcatcaa agaaacttgg aaatttttta ttgtgtttgt
     1321 attccacagt aagaattatt atttaatgtg tgatttatgt ctctcaggat tttccagtta
     1381 cttggggttt tttttctctt tttattgcta gattgttctc ttctcctctg tagacaggga
     1441 acgaatgaga tcttcccttt attttttatc aaatcttcag cctcaactaa aatcttatag
     1501 accttatttt atccaacaga gataaaattg ttac
//
LOCUS       KCVB01000118            1446 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5425pri ultra-conserved element locus
            uce-743, sequence.
ACCESSION   KCVB01000118
VERSION     KCVB01000118.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5425pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1446
                     /note="ultra-conserved element locus uce-743"
BASE COUNT          427 a          282 c          257 g          480 t
ORIGIN      
        1 aagatacaca gagtaattac ttaaggcttt ccttcacatc tctcaagact ttttttttta
       61 ttacccaaaa gctttttaac attagcactc tcttcctgac aaattaaatg aaatttcatt
      121 actctagcca tcctaatggc atgaaactat cagctgttgt atgcctggtc tctcttccct
      181 ggctgccccg gcagattaat caaatagtca gcctcagagt caaactgaga ggagcatcga
      241 tccgcaagtc tgtgtttgtc tgcggctcac tcgtgctgct gatccgaacc ttgagagaaa
      301 gtcttagata ttgatttcca tatgttaaac atgtacagtc catcacaaaa caattattca
      361 gagcagaggc aaatgtctac ctgctgctac ctgttctttt ccttgataga gaatgtatca
      421 aggcagcatg gttaacttta atgctgttaa tattacctga atgtacttga attactatat
      481 tcctgtttgc aaataaagta atcccattaa attcctgtag ggcattatca ttttttcgta
      541 ccccacttaa tgtgaataga tacataatga aatttaaaat atgctttaag tagcactcaa
      601 ttacatggag ggggcataca ccaataaatg gttttggtag atttgttata accagccatt
      661 acctatgata tttaacacct ttggttttgt aagggttaag ctgcttggcc tttgatctct
      721 caggggtaag gtcttggcaa agtgcccctc caccacccga ggccccttaa gggactttca
      781 atccaattgc tggctaagag tggatgcaga tctcaaggtt acacctgagt gttcaagggc
      841 aggtccaagt gtaatcggca cttagatcat agattaatct tatttgctat ctattaggct
      901 tgattcttgc ttccttctct cccctcccaa agccaggatt actgtgatag tttaagataa
      961 tggattctta tggaagaggg ggtcaagaac cattcttgtt tcagaacctg cagataatat
     1021 gattaatcta aaagcctctt tcaaaagagg tgccaccttc aaaggcctaa ttggcaaaaa
     1081 ggtatagaat aaatctgaga tgttggctct cagggttttt tttttttttt aattacaatt
     1141 ttattctctc accaggcaaa aggaaataga atttgtatct acctcgtgca cttatttatc
     1201 aatgggaaca tattcatcaa gcgaatcagc agaaatatac aaatggcttc tattaaatac
     1261 aatgtttggt aatctcatag ttttcagctt tcaaccttta gggtccttgt atatgtattt
     1321 tattgatata taatataaca tattgatgtt gatgacacta ttcggtacgg cataattgac
     1381 atttctgtaa tacaaataag ccagacattt tagtgagatg aacactgatt attactttca
     1441 agccat
//
LOCUS       KCVB01000119            1359 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5438pri ultra-conserved element locus
            uce-868, sequence.
ACCESSION   KCVB01000119
VERSION     KCVB01000119.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5438pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1359
                     /note="ultra-conserved element locus uce-868"
BASE COUNT          457 a          278 c          265 g          359 t
ORIGIN      
        1 acacacacac acacacacac acaagcaaac acagacacaa atgagaagaa gaagaagaag
       61 aagcctgcat agctcctaag taacccctaa cataaacagg caagtaatca atggagaggg
      121 caagcaggca gagagggccg ggaaaacaca gggtggagag agggagacag ggagagaaaa
      181 taaaaattgt tcaattactg ggcaaacacg ttttttctct ttttttgaac aggtgatttt
      241 gcagtgcaaa tcaatgttgg caggtcaggc tgcctgctac taatcttggc attacaactc
      301 tccaatcaga agctctcagg taatggagtt gttattgaac ttcatagtct ggattagatt
      361 ctcgctgaag atcaatgctg cgccaagatg agactgataa acccttgata gcaatctgaa
      421 tccctgaagc catggagtga agcatgctgg gctcaaatac agaagaggag gagggtgtct
      481 ggactcagcc ccccccccac acacacacac attctctcac ctccaaaaga ttggaacaaa
      541 acaactagta tctaacctca cacaaacagt ttatttacaa atcttgtgga cagaggaaac
      601 atgttatata ctgaagcaat agctcaggaa aggccttggt atgttgtgat tacaccacag
      661 ctaagaggca acacgaagca tctgttttat ccattttata gattttacat actgcatgca
      721 atactcaaaa atgtattcca aatgtcttat acaaacatct taaccttaaa tcaagcaaat
      781 aagaaacgaa gcctggatac acaatgttat tttattcaat gaacgcttcg aacaagctgg
      841 gaaaaaatgg gtaacaaggg gagggagagt taatgattgt gctgtaatct tcatgaggag
      901 acttttaatc cacacttttc tcttccattg actctaatag gattattgat agtagcatct
      961 ttgtttttct ctttttttcc ccctcaactg ttcaagaaat gaactcgcac agcacatgat
     1021 atgacaatgt ttaccccctg tttgcaatat ttaaagactt ttgacatatt tattaaaact
     1081 tatcaaaatt ggaaaatatg ttgggggaaa taatgaatac aaatactttt caaatgttcc
     1141 acaattaatt agaatatttt ttgagcctgg gaaaatgcag cacactgtcc accagcttgt
     1201 gacatggaga ctgaactcca aaagcatcta tgtgcatgac acatagctca tttttgtttc
     1261 tgctctggtg gttgctatca atttattcca gcgactcagc caccatttag cattaaccca
     1321 gctggggcaa caaaacggag cacaatcgat gttggatca
//
LOCUS       KCVB01000120            1743 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5475pri ultra-conserved element locus
            uce-1141, sequence.
ACCESSION   KCVB01000120
VERSION     KCVB01000120.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5475pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1743
                     /note="ultra-conserved element locus uce-1141"
BASE COUNT          556 a          390 c          384 g          413 t
ORIGIN      
        1 cttactgtat aaataacatg taactataca atgacgattt cacatggaac agaaaacata
       61 accataagta tactattaca gtaaaatgaa ttctgaaaaa tgtattttgc atgcttttca
      121 gcccccttta gtgtgtaaca tgaaggcccc gggaatattc aaatttatct ggtgtcagtt
      181 gaggtagtgg ttatcagtgg ttcgtctttt aaggttgtgc agcagactag accctgggta
      241 tccatcacta tacaggttgg attgaacaga gattgacagg tgatatgctt acatgtggcc
      301 tgcagtatga gcaaatgtga gaaattgttt ttaggggtcc cagtcaccac acagcctacc
      361 actgactgac aagccaggtc actaactgga cacttctcag gactgttttc catctaaatg
      421 ccccacatgc cccagaaagg agaggatcac aggagcaacc acataacgac taagaataag
      481 agaaagaaaa aggagaggag gaagagggag gatgaagaaa gaggcttgta gtaatgggga
      541 acaacaggaa gtggcaggag aggaggagga ggaatccatc aatcaatcac acaacactga
      601 aaacatccaa tcacatgaca attatatggt catcactgac acacaagaaa acacaaagcc
      661 accgggacgc acacacgggc tcaggccggc tcacgagacc gcccgcacac tgaggcacca
      721 ctatgagagg aagagctgtg aaagggttaa agatgcttac tctattcccc accctgtgcc
      781 tccaaaaatc acccccaggg ccagaatggt ccctgctact gcccctatca gcctgttagt
      841 ggccacgctc actgtgcaga gggaaaagga gcattattac caccctgtca aaaacaaaca
      901 gcaggcttct gcttcagagt caaggatgaa gtgacacggt tcccctccgg gatgcttccc
      961 acccccttaa accacctccc cgtccccctg tggtcaaagt atcaggtgta cagaacaaca
     1021 tatacagtat atgagccagt gtgaatcttg gctaagttaa atgaatgaac atagacacgt
     1081 gtgttattca ttctatcaga tgccagaggt tgaaagaggt ggaaaccgac ccttgggtcc
     1141 ttcgtcctga gcggtctcag gctctttagg cgggtcaggc atgctacagt ctgtccccgc
     1201 ccaggtggtg tcacacgtgc atgtggcttc attgttgcac acctgtacag accagacaga
     1261 aatagataag aagacgaggc gaacgtaaga gctgtaaata gaggtgctga cacactgggg
     1321 agtttttggg aaccatcttc actggtgact ttctcagctg tcttcacatt cagcaattat
     1381 acaagattaa tgttcatcca cagttaatac aggctcccat atagacactt actacattta
     1441 aagacggtta aaaaccataa aggttaataa agataaagac acactcctga aaactgatct
     1501 tgattaatta tatcacagct ctacaatggc attttttagc caggtcagct ttgtaaagac
     1561 ggataatagt aattcaaact ttgcaaatac agccagaact taatctaatc ggttgaatta
     1621 cacaacaatg gctggaacca atcaagcaca atgggctgaa gtccgttatg tttttctcac
     1681 agcattttaa accgtagtag taagaaaagt actgttacta agtgtaactg atatcatcaa
     1741 cga
//
LOCUS       KCVB01000121            1401 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5519pri ultra-conserved element locus
            uce-173, sequence.
ACCESSION   KCVB01000121
VERSION     KCVB01000121.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5519pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1401
                     /note="ultra-conserved element locus uce-173"
BASE COUNT          502 a          273 c          305 g          321 t
ORIGIN      
        1 acacacacac acacacacac acacacacac acaaacacaa gcagagagac atacaaactc
       61 aaaaaccatt gagaatcttt tggcaaaaac aatgaaagga catgtgacgg atgtttgtct
      121 ctctccataa agaaacattt aaaagtgcaa acacattcag atacatatac aggaacaaca
      181 gctgcatgca caaagtgaag catacttgca ccaagttgaa gtaataaacc ttgcacactt
      241 gcacacactt acacacacaa acacatccac tttcacaact ccgccaggct cggagtgagc
      301 tagcttaact actgggataa tttccctcca gcaaatggtc actgtgccct tgaaaaacat
      361 atttgattat tactttttct ccccctcctc tttttttttt cttttagcat catcacagcc
      421 attttcatca cgcgctctga tgatggcacg gcaaggcgcg ttaagataac accccggcac
      481 cgccaggctt ttaacactta aacggtgaaa agggatattc agatttgtcc ttgaacgttt
      541 ggatggggcc tgctgggctg ggacagaggg agggagcagg ggaaggagtt aaaaaaaatg
      601 catacatttt caagattcac ttactttaag aaaaagtgag gtggggaggg catttatggt
      661 agcaaccaca gtgagagatg ggaaaggtgg gattgaaggg cagatggaag agttaaataa
      721 ctatacattg caaaaaaagt gtgaagggga aatgattagt ggagtacgca cagtgaggcg
      781 ggggacttag ggagagtcgg ggagcaatgg gaggccaaag acaccgcaca cgttccttta
      841 aacaccatct aatctcaact aagtcttagc taaacatctt acatacaaac tcagagagag
      901 aagaaaagag gagcaaatga agagcttaaa agttggtctt aaacctgcaa cacaggagga
      961 aatgctgcaa gaaaactgag gcagtcttgt ccaaaaacaa acagtattta atctactcga
     1021 aagtgaaatg aaagctgtgt aaacaaactg gcagatttgg aagggaggga tttagcatgg
     1081 agactttgtg ttattgtata acaacaaggg aatgctgagg aaataaataa acaaataaac
     1141 tatctcagta ataccccaaa ctgacccaag ttgaaactag gtctaccatt taggatggta
     1201 tatcagagtt acttaaccag gtaagtttga ctgaagcaga aacctataca aattgatgta
     1261 gttaggctca taagctggaa aaaaaggctt gaagcctgaa aagagcacct atgcacctaa
     1321 agcagccatt atgtggctgg tgtagatagc tgtgccattt aaaaatttaa atcaattcca
     1381 tcagatgtaa aatggaatac a
//
LOCUS       KCVB01000122            1430 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5559pri ultra-conserved element locus
            uce-975, sequence.
ACCESSION   KCVB01000122
VERSION     KCVB01000122.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5559pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1430
                     /note="ultra-conserved element locus uce-975"
BASE COUNT          479 a          267 c          325 g          359 t
ORIGIN      
        1 gttgctttct gcgggataga agtttttact ttgaatgttt aaaagatctg gactcaaaaa
       61 tgtgtgagag aggtaagaaa aactgccagg gagttttgga gaaaacacaa aaaccattaa
      121 aatgctttgt acacagtgaa ccataacaca tttggggctg tgaatcaagg gggaagaaat
      181 catgtacaaa aagtaccttt tgagttgagg taaatcaaat ttgcagtcca aaacatttag
      241 cagttggtct gttctgttct ggggaataga tgcatgaaaa tccctctctg agacgcatta
      301 gaaattatac ccttgggatt agcaggtcag actatggtta cgcatatgta cgttacataa
      361 accataactg acacatgagg cccatgtatg gtaaactggt gcaataattt gtccagatgt
      421 agcagacctt aaatcacatc ttaggcacgg atgagcacaa aaggttcttc atgaatgaag
      481 gtggatgtgt caaaacaaaa gctctgccaa accattgtaa acactttgct cattatctgg
      541 cattgtttgc aaaaatacga gtctagactt ctatgatact tgcatgcatt acactgaaag
      601 ctaggagggt tatcggcata acagactcac ccacaatacc cggattcaca aaaggaatga
      661 ttgtggctgg aatgtggtgt gttcccagga agactagaga gggccatcac aaaaggaaaa
      721 cccacacggt gggcactttt tcttctgcca tgtacaggaa gcaaaatgag aactatcaag
      781 tttgacacta ttcacagggc cttcacgggg ccctacaata cctggctgag caggcagaat
      841 gccgggctta agaaaggggg gaaaaaggct ggcaaagtct ccgtcacgtg aaagcgtgtc
      901 gatatttctg acacctccag gctacttgcc aaataaagag cataggggct tgtcactgag
      961 gggccgccgg gcccaccaga atgctgaaag cttgtacccc tgatgctcca acagagacct
     1021 gagatcgaga atttctgtgt aaacaagatt caaagatctt gaaatgcaaa agtggtaaaa
     1081 actgaagaaa aaaaatgaga aaagcaaaaa agaatagtaa aaataactag taaatagtaa
     1141 aatagtaaat agtaaaaata aaaagcttta tatgaagact ggaaacaatg agccggggtc
     1201 tctctacagc tcagagagcg gggtcacctc tgaggcattt ataggtttaa ctcatttgtt
     1261 aaaaccaaac caaagagtaa aaacgacaag ctgtggtttt actggaagtt ttgtgcggga
     1321 gggcactgac tttctggact tacttatttc tgtaagctga ctttcgacga cctgcatcga
     1381 tctcatccac gcttgtcaaa aataatgtag tcatttttat atttcctgaa
//
LOCUS       KCVB01000123            1523 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5603pri ultra-conserved element locus
            uce-136, sequence.
ACCESSION   KCVB01000123
VERSION     KCVB01000123.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5603pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1523
                     /note="ultra-conserved element locus uce-136"
BASE COUNT          411 a          302 c          375 g          435 t
ORIGIN      
        1 aaaatagatg aatacatacc agcgattcaa ctattcccct tttgtctcag tgctgagatg
       61 tctgcagttt ctccacagag ggatggcagg ggtgaaaagg agtggcaagg accactattg
      121 aggagagtgg attatctcat gaagggaaat caattagaac gttacggtgg actgtgaaca
      181 tttatccatt tagcttgacc actattgaat ttccatgatg ggctttgact gttcagaggg
      241 agagatcaga gttaatggca attgttctgc actcctccaa atgtattatc tgccttattc
      301 aatgagagac acttcatcaa accatagaga gccctgcggc ctattgatag atgggctttg
      361 tgtggggttt gaagaaaaaa gatgaggcat gggggttgaa ggctgagggg agaaatggga
      421 gggagtgtgg ggtttgaggg gggtagtcac agatgtccag ccctttgaaa gaaaattaaa
      481 caagtacctc attagccaac gctccagtgc tccctggagg tggccactga gccaggcttt
      541 ttaatgagct gggaacttaa atgctgccaa agtttacccc ggccttgcct ttctctgtta
      601 ggccactggg aagagccgtg ccctccccct ttgcccctgg tcccctccat cccccacctc
      661 agcccggccc tccttttcca ttgagggccg ctcatgcaga ggagagagag gcaccacgct
      721 gattccctgc ctttcagctc cggagagggg gataatgctg tttttgtacc acgcatccag
      781 aggaattatg tgcgctaatt tgattagggg aagatttgat taactggaaa atgagggctt
      841 tgcttagact ttcacacgct taatttatcc cttgcaaagc agacgcaaca ttgtgtcatg
      901 aaactcagaa ggcatatgag aagcccattc accagctaga tggccctttc agactcaaca
      961 atatctagga ctgccacggt tcaaattggg ataatcagtt tagagctttt tgcctggcct
     1021 cagaatacaa attgcatttg gcattactag tgaaaaggtt caaaggataa tgaggttaat
     1081 gtagcaggga atggagctaa ggcagaaaaa tttcagaaga aaatgctaaa ctagtggcat
     1141 tgttgcaaga gttggatgtg atggtttctt tcctttctgt ggtttatgtc tgtgtgatac
     1201 cttctgtggt tgctgagata agtgataagt gcattagcag ccaattttaa ttaagtatac
     1261 aggatgtcaa acccaatgta taaatattag aaactatagc agcatttaag acatttccaa
     1321 tgcatgtgca tgtacatttc tttgcaaagt gaaagagcca ttattcttcg atgctttgaa
     1381 ttcatttagt ggttttcact tgtctctatg atccctttgt atactctgga atgaccacag
     1441 ggactgaagg agctggatgc tttgtttttg ctctttttaa catgcagcca tgaaggtcat
     1501 attactgcgc ggtgatgatt aca
//
LOCUS       KCVB01000124            1809 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5648pri ultra-conserved element locus
            uce-1205, sequence.
ACCESSION   KCVB01000124
VERSION     KCVB01000124.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5648pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1809
                     /note="ultra-conserved element locus uce-1205"
BASE COUNT          491 a          344 c          400 g          574 t
ORIGIN      
        1 aatgacaaca acaacaaaat gatcatctgg attgttgttt agctactgta ctgctgtgat
       61 gctactctaa tttaattaat ttcatagatg ataataataa caatatacca atatgtgagt
      121 atatgttatt accccactgt cctcgatgat gttgccaact taagctacaa gacccagcta
      181 ataaagcctt tgtagtatat aacaagttgt tcagctagcc aggcttagag cattttaatt
      241 aaatagatta aaaacaatgt tatggtttct tcatgtggta gccttcacac aatcttttgt
      301 ttctctcagg ttgaaaagaa agagtgggag aggggttgag ccaagattgt gtttccaggc
      361 aggaaagttt gaagttcttt gtttggaagc aaggttccgg gtgcagcaca ctgtttagct
      421 cggtgaccct gtgactgtct tctgaggaat tgtatgagca gccctgcaga attgtgcttc
      481 agggtcagct ttcatcttag cagtaacctg tggactttga aactcagggc gatggaattg
      541 cagctttgtc agtacactac taattagaaa attcctgacc tcgagcaaga tgttttaatt
      601 atgtgttgtg tcaacgagtt tgcagattta atccaccgtt gatacacact caagtgtgca
      661 aacggtgtcg cacattgtag tagatcaagt ggaatggtga aggggctttt tgctccatct
      721 ttctctgccc catataacct tacgcctctc ctgcactgtg tttgttaaga gccgtcacat
      781 gcttaagtaa ccaaattaga cgtggagtta atgtgttagc tcatgaaaaa gctggtctga
      841 tttgccttgg tttgtggaag gctgaacaat gtgacagtat tgtgctgggt gtgagctctc
      901 tgttgcagtg agtcagtgtg agggaaggca ggaaagggtt aaacagaaat tgagcgggga
      961 tgctgggggc agcagtgctg cagaacatag cagtgttcgt gcgaaggcaa gtcatttccc
     1021 gaaacactgc agacacatgg cctgcggcgc tctaaaacct ttggagacca aaatggcttg
     1081 caggagttag aagtaataag gtgttttttt gccttctttc tccttgactg aaattctgca
     1141 cggttgctat gcattgccat ggagctgtgt agctgttttt atggtttatg tgggtattaa
     1201 gatgctctgc aactggccat ttttgagatt tgttggtctt aagctgtaaa gcaaatggaa
     1261 gttgtttgtt ttgaaagtaa ttccctcatt ttttctgcca tgatcagaat tctagacctc
     1321 acttttattc ctttgtattt tacccgtttt tctttctgtt gtacacaaaa tgacagacta
     1381 acaaaagcat ctatgaaaca agaaaaacag atccagcaaa tctgtttcac tatgctcctt
     1441 tttttaaaaa gcacaatgaa aatggaaggt tcttgttcag acttttccca ataaggaacc
     1501 tgtgatagaa acaaaggccg cctgtgtcgg tgcttgatca taaagctgtg gtattagtcc
     1561 atcctaacct ctctgattat cagtgggctg aaccaaaacc ttttcatcct ctgccctggt
     1621 tctgttaatc ttggctgcct tgtctgcttc aaagagaggt ttcagcatat aatctgggta
     1681 tgtacatctg tgaattatta ttctaattct ttatacgttt attagagctg aataaactcc
     1741 acatcaaata catcacccgg gcaatgtcag aaagcccggg gcaaatcagc cgacatctgt
     1801 taactcttt
//
LOCUS       KCVB01000125            1473 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5690pri ultra-conserved element locus
            uce-1240, sequence.
ACCESSION   KCVB01000125
VERSION     KCVB01000125.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5690pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1473
                     /note="ultra-conserved element locus uce-1240"
BASE COUNT          476 a          247 c          250 g          500 t
ORIGIN      
        1 ttttctactt ttttctagtt taaattagag caggtgctcc acaatataaa gttgataagt
       61 tggaataata cattacattg ttacattttt gtttataaaa ataatgttaa atataattta
      121 cgtacaatgt acaattgtgt tttgcagatc tctattttga aagatcattt tcaactttct
      181 tttactgttt tcaatgtcag cttttcagac caaatattga gttccccctt atgcagcgtc
      241 tcttgttaaa taaaaacata atataaactg tagagtatat tctgttaagt gacaaagaca
      301 taacaaactc ttgctaaatg tacaaattaa tgagtattac cgttgttcaa cattaaaaat
      361 acaattccct tcttcactca ctggatttct actaagtggt tacctgaagt aaagcctata
      421 ttcaccataa gttaaaatga agtgtgagga gtttcacatt gtttttgatt aacattgtgc
      481 gtttagtctg taagctggta gacgtggatg ctgcctgtgc tgtgacccgc agtccactct
      541 taagtaaaac agcagacgtt aatgagcatg aggacaaaag gctgttaaaa cacacgttaa
      601 catgaaactc acaatgcatg cacacactca cacactcgca cacacataca tacacacagt
      661 tgtgcctcct gtttcccatg gctagtttcg ctgtctttac atgtggggca cgtttgctaa
      721 catttcttaa ggcttggctt cattaatgca gacgattagc atcctgtgaa ttaattagtg
      781 tcagtgccct tcaaaatgct aatgctagca ttctgttgtc ttaaagagca gttagagagt
      841 ctgggcgatg atgcaaagtt cttatttggg cgtcagctgc ttcgatttca atcagttaat
      901 gaatgactat ttcaaaagtg agttttctca acagtgaggt cagcttttga tggataatta
      961 gtcttgggat taattaattt atttttaata aagagatttc agctccagac agaattagaa
     1021 ccatgcaatt tccaactaca gattaaaata tatatagtag tttaactttt agtgaatcag
     1081 tcatctgcct tttctagcac agacagacag taaccatgat tttattcctg caagctgttt
     1141 ttatgacatt cctgacatgt tgttcaatat taataaaaca aaaaagaaac acacaaaatt
     1201 aaggtgcagt gttgatatca aacaatatat ggagtgacca ggatgaggat ttttttttat
     1261 atctttataa gataaatatt tttgtttcct ggttgttgat cagataaaac aaacgatgtg
     1321 cagatgtcac aattcacaga ttcactgatt aataaagaaa ataactgtca gattaatcaa
     1381 taaagagatg aattgatagt tggcctagtt ggttcaacag ccttcagaca cagtaatagc
     1441 aataagtttg acattgttct tttcaattgc aat
//
LOCUS       KCVB01000126            1228 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5719pri ultra-conserved element locus
            uce-272, sequence.
ACCESSION   KCVB01000126
VERSION     KCVB01000126.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5719pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1228
                     /note="ultra-conserved element locus uce-272"
BASE COUNT          353 a          219 c          243 g          413 t
ORIGIN      
        1 atttgctctg ctgctcacta tctatctata tacagtatgt ctcagtatct atcacagtgt
       61 gatttaaaga agtctgtctt ctgtgtttac cgtcctcaag actatattct aggtaatttt
      121 ttaaaattaa gtatgccttg gaaatataac actcattgta cttctaataa ttaaattaat
      181 agtttgctga attgttcctt tttactagca atttttctca tgaaaacata aatgtaaata
      241 ttgtcaatat tgtgtacttt tctgtgattg acaagtagct ctcctgcact aaaccccacc
      301 cacctggcaa tccatgcttg gttaaacaaa cacaacaatt tgcaaacagc atttctgctc
      361 gaaatcgctc agtttttttt catcttctga ggcaaaaaaa agcttgtgtg catgtgtgtg
      421 tccttgcaag aatgtgcaag ctaatacata tttgcactac tgattgagtg tgcagcagaa
      481 tggctctatg tgtgaatata tgcatgcttt aggtgtgttt ctaccagcac aagcctgcac
      541 gtaccgagtg tgtgtagcta cagtaagtgt gtgtgtgtgt gtgtgtgtgt cagcatcatc
      601 gctccatctc ccacggcgtc tcctggaagc tgacaactgg ataatagctt tttacttttc
      661 cagagcaggg ctccggagag aactaatatg attataaatc attattcaaa tagaaaggtg
      721 aggcgctcgg gatagtaaga gagcatgata atagcatcat taccttttat ttataattct
      781 gatttttttt tttttttttt ttcttttttg caaacgtggc aactcaacct aatgcaagac
      841 tacaggggat ttctctctgt gatacggggg agctgtagat gcatcaaaac catcctggag
      901 gagaggagga gaggtaaaga tgaagttatt tgaaagagaa catttataaa aggttttgaa
      961 gggatttagt ttgtagctag gatgaaaatc ctttacatgg gaattttggg ttttagtgtt
     1021 tatttgcttt ccggaataag ccgtcccatt agatacatgt tagggggact ctgacaattt
     1081 agtattaggg aatttgccag caacagactc taaaaacaat gatcaaaatc ttctttttat
     1141 gttagaacaa actgtgcaac caattcctct caggttggct cagtgactta gtcatcaggc
     1201 tcactcagat catacggcat atatacaa
//
LOCUS       KCVB01000127             903 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5802pri ultra-conserved element locus
            uce-1078, sequence.
ACCESSION   KCVB01000127
VERSION     KCVB01000127.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 903)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5802pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>903
                     /note="ultra-conserved element locus uce-1078"
BASE COUNT          265 a          186 c          158 g          294 t
ORIGIN      
        1 attatgtaga tgacaattca cacaaaagtt cccctgatgc attattaaga gggaactgca
       61 gacaaacgta tttctcagcc aaagacgacc aagcaagctt tacagctgct gtgaaaaata
      121 ggtacactgc acactgtgga ttggcacagg gcatattaac actaatgttc cccttagaac
      181 agatcatgaa ctccagctaa tgcttccctt agaacaaaca gtcagactct gctttattta
      241 tgactgcaaa gccatgaggc catcggtggc cttttcaaca aaggaagaga gattgctcat
      301 gtggcactaa atagtcatca tttgttttgt aaatatggag ttaatgttct gttttaaata
      361 aagtttatga ttggagctat gaactctgtt ccgtttgttc aagggacccc aattacaatc
      421 tctccttgat tatcccacaa tgctgaaaac aatgggaagt taaccaggca caataataca
      481 tcattaatgc ccattttgat caataccctg catgcaaagg ggatctttac ggcacctata
      541 ttatagacca ctgggatctg ttttgctcat taagctggtt aaatgtgatc taaataagat
      601 gcccacctcc cattacctgg ctaagctgtc gttgatcgtt tatgcgatta aatatagacc
      661 taaatctccc tcttatccaa ttaattccct ctgcaggcat gagagtgtct aatgatgtta
      721 tctttcctaa ttaacctgta tagggattca actccaggac aggggattgt tcatccttca
      781 tccaagtgtt cattattatc attacaatca ttatcataaa ggggctaact tatgatgttg
      841 gccattccat caccattaat ctagattgag tccttttttt tttttttttt ttttattgtt
      901 ttt
//
LOCUS       KCVB01000128            1298 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5842pri ultra-conserved element locus
            uce-722, sequence.
ACCESSION   KCVB01000128
VERSION     KCVB01000128.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5842pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1298
                     /note="ultra-conserved element locus uce-722"
BASE COUNT          391 a          285 c          262 g          360 t
ORIGIN      
        1 atattcctat tattcagttt tcgtaaactt tgttgatgaa aagaaaatta aagagttatt
       61 aaagaaaaag tgttgcatct ttgactcagc aaattgagac atctgctcaa aattaattta
      121 acatgctgaa aaaaggatga gaatttaatg ttttctattc ataaactttc tgaatataca
      181 cattcctaag ttctgacctg tcagatttac tcccacatca ggttaagaac tacagtagat
      241 acacaagagg gagacacggg gagcagagca tctgataaaa atagaggtca taaggtattc
      301 ttcatttcgc tgagtgctac agaactggaa ccacttggga tgttggtata taggacaccc
      361 gctgctaaaa caccgccatg cagcaccatg attacgactt gatccctcct ccactgaaag
      421 ctcacgctgg cggaaatcaa gtaccccgag gcttacagca cgcagcccaa ggcctaaaag
      481 gtaatagcat gaaatataga ttcgttggtg acagatgttt acagtgcgtt gcgagatggc
      541 attgagacaa gatgcagtgg ctccctccat tggagaggaa cctgatgagt ggtatagtga
      601 agagccggag tgatatagga ggtgctccga cagtgtttgc aggcagatca ttagggcccc
      661 tctttaactc aacccccagt gacaatctat tgctctcctc ccctgtcagc gaatcattat
      721 ggtctgatca ttagtgacat cgccacatgc ccatcactca ggctggtgcc atattggcag
      781 atgaacagcc tctgccccag gcccactaat ccagacttca gacagggagg atgaaggaat
      841 gttcgccgag catcaaaagg agtggacgca ctcgtttcac gccgaccaaa ctcagacttc
      901 tcagcaccag gagcagacat ttaaaatgaa gtgcacaaaa ttatgtcttg atctttttgt
      961 ttttgctttt atgagaagta tccagacatc caggtgcgta tgtttatgcc atgatagtct
     1021 cttatgacac tttcataaag tctctgtctc tatgaggtct ctacaacagc aggtgaggga
     1081 tgaaactcac aaacattatt ctaacttaca acatgtaacg tttccacata aaaatgtcta
     1141 aaaacgacta actatgttat gtattttgtt ggactgtgat cttaaattat ctcaaatttt
     1201 tccaacaata ttcaaactca gcgaaatctg tcattttctt caaggtaacg tttcactttg
     1261 tctccttcct ctaacttccg gggtaacacc agccagtc
//
LOCUS       KCVB01000129            1665 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5881pri ultra-conserved element locus
            uce-767, sequence.
ACCESSION   KCVB01000129
VERSION     KCVB01000129.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5881pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1665
                     /note="ultra-conserved element locus uce-767"
BASE COUNT          537 a          321 c          290 g          517 t
ORIGIN      
        1 gacctgaaca ttgcaaacac tgtactttat gttttgaccc atccattcag actggatcaa
       61 atgatttgat gccatttttt agccacactt gaaacaaagc catgagtttt gagcaactgt
      121 aattgatgcc aaaagataca tatggcacct ttaatgtgag ttatgtaact aagtgcaatt
      181 attcaataac tgtacttaaa ttaaaattta ctggagaatt tccattttat tctactttat
      241 acttctacac agctatattc aattgactaa tgtgaattga ataatacttt tacttaagtg
      301 acttttgata tccaggactt ttaatgcagt atttcaaaag cagtgcagta ttgcttcttt
      361 aactttagta aaaaatctga atatttcctc tactcattcc gtcccttgtg tatcttttag
      421 tgctttattt aatctaaaac atcccaaatg tacagcattg cacactctat ataataagca
      481 attgttctgt acggactcca caataaacct taagtgcaca gaggactggc gtgtactata
      541 tcttctaaag tctgactctg agaagtctct gagaaattca ggtctttgtg cgtttggcat
      601 tgagtggaac tgcatttgtc ttaatgatgg tgctccttga gctgattttt gtttcacaat
      661 aatcaatatc aaagtcgcca gtggcagcaa tgcccaagaa aggggggagt aaatgtaggg
      721 aggcattctt tctctgcgtc ggccttgtca atgtaacaat gcttttagag tttggccctg
      781 agcatctaaa cacactctca atcactcctg aaatcctagt aggccacact tggctgctat
      841 tgtaacttag atacagtctc aatttgtatg ttgtctctca gtagacatgg gaaaaataaa
      901 tggattgatc ttccgtccca gtccagcagt tacagcacat cttttttcag acaggattta
      961 taaaaagtgt caccgctgac aaataaacag gtatgttcac aacttctgga ttatattgtt
     1021 atcatacctg tatgatgttt tcacacttca ttcaactaac atactgtaaa aactcagcct
     1081 caacaccgaa ctgtcttttg cagccatttg aaaaatgtga gaaattaaaa taattttgga
     1141 aaattgtggt caatgcataa gaaaaaaaat aataatcata gctgatgtaa aagatgatat
     1201 gagagaccaa aaagatgaac acaggatcaa gaataagaaa gagagtgata ttatcctata
     1261 aatcatcttc taattttttt actatataag aattcatcca gtcataactc tatcttaata
     1321 gagtcataat ctactgtata tccctgcaat tacccaacat aaaaactcaa acaaacagag
     1381 gctaatgact ctatttacac agctatgatg ggcctacaaa caccatgagt ttgtcagaga
     1441 tactgattgg cttcatctca ctgcactgag taaattcaag aatgtttgaa acaattcagc
     1501 attggctctg gatgctaaaa ctgcagttgc tcacacaata aaattcagat acatttcccg
     1561 gcgatattac tgtaatcaga tttattcaga ccgcggtccg atgaagcaag gggagactgt
     1621 gagaggcgcg cagccccgtg tggcccctca gtgaccagag aggag
//
LOCUS       KCVB01000130            1755 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5925pri ultra-conserved element locus
            uce-1085, sequence.
ACCESSION   KCVB01000130
VERSION     KCVB01000130.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5925pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1755
                     /note="ultra-conserved element locus uce-1085"
BASE COUNT          481 a          362 c          466 g          446 t
ORIGIN      
        1 tcatgaacat ttgcacacac acatcaaatc tctgcaagcg cacacacccg tatatttata
       61 cacgttttgc agagggatgc gctttccgaa aaacagacgt gtgcgtgcat gcatgcatat
      121 atacagtgca gtgcatgcac gccaatggct ggcagagcat cttctttttg acacctgcaa
      181 ctagttgcaa tacatcactg tcaggatcgc tttcttcacc tatccgcatt tgtgcgttag
      241 ttatccgcgg aggccttcgt caaaaagttg agcagtcttg acatcagctc cctagtggga
      301 acagaaaagg tgaagttgtg ctgggaggtg ccacggcggg tgtcttcacc ggctgtactc
      361 tctgcaccca acagcatcct aatgacaact gaagagagag atgatggttt aacatacaaa
      421 tattatgcca caattaattc tgcaactgac acatgatgag aaattctgaa cggcaaaagc
      481 aaatatcttg ttttgttgct ggtatttatc acgagtcaca ctgatcactg tactgttctg
      541 tgcatttact gtattcatga aatgaaggct agggagtgac ataacaaaaa gccaggtaga
      601 ggtttatttt gtttagggcc tcttggcgtg aatgtatgag cttatgtaat tgacagtgag
      661 tgtagctatt gaggaggaag tgagggcacg gcgcctcctg tgtgagctca ctgtggagag
      721 gaggagaggc tgccggtggc actgctagtg gtgagcacca acagctccga ccacgacata
      781 agagagaagg tggggagtaa agggggaggg gggggtcagt gtctgaactg gctgatggga
      841 gccagaacat gaaacaatgc cgggggaacg tctggtgggt tcgctgcaca gaattacaag
      901 cgagggagca ggcctgctga ggcaattaca gcttaatggg gtcatttgga aggcaattgc
      961 caggggttaa tgtagtatgg agagatgagg tgagattgaa gtgaaatttt tggctagaaa
     1021 atgtgttcaa atgaaaaggg agcaggcaga caagttgtgc actgatgggg tgggaggttg
     1081 attgggggaa ctggaattca ggctttcatc gccacacaga tagacgcaag gcctggtcac
     1141 ccactcataa gtacaccagt gcagacaaat gcacatgagc atgtccatac aggtgcttta
     1201 gctctctttc tcatctcttc ttcagccaag ccagtgcaca cttatctagt ctaataagag
     1261 ctacactgtt ttgtgctgct gagcactttc tgttttactc acagacatac ctagcagtag
     1321 ttctcttttg tcttgaatta cctgtgattg tgtttcctgt attgggaaca ccaaccagca
     1381 ggacacaaaa tagaaaacaa aactgttttt aagtgaaaca gagaaagaca tgtcagagaa
     1441 actacaagag gcacctcgga gaacgaggat aaaaggatca ttatgtgttg ttacgttagc
     1501 caggtcatta ccttaattgc tcaacataat ccggtgttgg aagggtgcac tggagctttg
     1561 ggaaggagtc tttgccagga cctcgtagcc caggtctcat ctccctgatc tgtagcaagc
     1621 agaccgatcg tgctaaactt tgatcaaagc caaacttatt taaggtcagc catgtgaaat
     1681 cccctgggag ctgccgtgtt cgccgaagcg gactgcggta taggaagtag tacagcactg
     1741 tgccttgctg ctgct
//
LOCUS       KCVB01000131            1572 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 5967pri ultra-conserved element locus
            uce-423, sequence.
ACCESSION   KCVB01000131
VERSION     KCVB01000131.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="5967pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1572
                     /note="ultra-conserved element locus uce-423"
BASE COUNT          474 a          339 c          290 g          469 t
ORIGIN      
        1 aaaatgcctc tcttaggtgg aatttcgttg atatattatt atattaacat tcgttgataa
       61 cctacatcat ttttacattt gtttctaatg tcacttgtgc cagagggagg cgctgttgtc
      121 tacaaatgca ttctccaacc tcagccacac acagcagcta acactgtttt ttttttaccc
      181 cgcaaagact gaaatccatt tcatgctcgc aggagggcgc cagctacaag agcgatgaaa
      241 tgagggacgt gaggtttgat tcctgttcct agaatgaggg cttaaaataa taacaataat
      301 taagactgaa gcaaatatag aatcctagac aaggcctaat tggatttaat ggtaataaaa
      361 tatcgattac agagtcatta tggagaccac aatagcctgc tcttaatttt ctccacagct
      421 caaccacatt acgtaagacg gatttgcgtc tgccatatag gatagataat tattagtgat
      481 tagaaattaa gagaggaaat gctgtgtgat ttttcattac aaaggaaaac attaagggat
      541 tgtacaaatt aaacaattct gtttaactgc tcatggacac tttcccaaca gctcaagcat
      601 tgtagccttt aaaaaaaaat gtatatatta atagcaaatt tataacatac gactgcactg
      661 tgggaatctc tacccgaaag caactctctc tctctctccc tctctgttgg cgtggttccc
      721 ggcccatctg cggcctggcc cgtggattac tgtttgttaa tgtttcaatc agctgtgtgt
      781 tgaggaatgt ggagctattt aacctgaact tccccaggtg tgccgaggcg ccgctcagtc
      841 tgggccccgc cgccctcccc tgcccttggc acaaagctat cacacaaaca caatcacaca
      901 ctggccacgc cggggctgca gctgtgcgca tcgcaccgca gggaaatggg aaaaacattg
      961 caccagggag acagaataaa tcttataacg aatcgcctcg ccaaagccag tttaacccct
     1021 gcagtgccgg gagacacgaa cactgctgat ttacacggta acatattgaa acaccatatt
     1081 taaatggatt attttatgga gtgaaacaaa cttttttcgt acttaattca ttctttcgct
     1141 cccagtaaac catcctatgt cgctttattt gtgtcagtaa gtcactaaaa acatgtacaa
     1201 acttaaatcc tctttaaaaa aatagtgttt atttaaattt ttttaacttt attttaaaat
     1261 gtgccttcat tcgtaaagat ctttagactt gacggtttaa agtgagactt cctcttgtaa
     1321 tgtgatccgg taatgagcca tagagtggca aattaccaat ttaactggct ttgagccatt
     1381 atgttgacaa ccagctttgg ttttatgtta cctaaatgac ttaacagctt cttcttggtg
     1441 tagcactttt taatgacaac cactttaaac tcccctagca ctcatgtcag caacacgaaa
     1501 atctattcag atgaatatga gtgggcctgc ttttaatcac aatgataata tggctataac
     1561 agcccataat aa
//
LOCUS       KCVB01000132             468 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6008pri ultra-conserved element locus
            uce-466, sequence.
ACCESSION   KCVB01000132
VERSION     KCVB01000132.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 468)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6008pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>468
                     /note="ultra-conserved element locus uce-466"
BASE COUNT          123 a           92 c          138 g          115 t
ORIGIN      
        1 aaaaaaaaaa acaagcaaat ccaatgcact cgggggggaa aacgacacgc aacaatagca
       61 tccaccaata gcactgtcaa acaacgccat ctctctcgga tgttaaaagc tttaagagct
      121 gaggcctctg tgacacccat agcctctgga tgtcaaagat ggttgaagac ttgagagcag
      181 gcctctgttt taaaaatact atgtgtgggt gtgtgtgtgc gcacatctcg gagcagcaaa
      241 agtgacattt gaggggcact ttacagaagc tggcggttgc tagggatctt ctgtatcacc
      301 taaagagcct ttgtcagtca ttggaaagct gtcacctttc ggggggccga ttcggtaggg
      361 ggagccggct gacaggtagg aattaatgag aggtgtatga cagcagacct cggtgctcct
      421 gtgtatgtgg gcatgagtgt gtgcgtgtgt gtgtgtgtgt gtgtgtgt
//
LOCUS       KCVB01000133             812 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6063pri ultra-conserved element locus
            uce-1264, sequence.
ACCESSION   KCVB01000133
VERSION     KCVB01000133.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 812)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6063pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>812
                     /note="ultra-conserved element locus uce-1264"
BASE COUNT          197 a          141 c          188 g          286 t
ORIGIN      
        1 gtcctatatc aattgcagca ctggtggcca gctttatgct taagtgcctt gatcaaactt
       61 acaacatgga tagaaattct ctttatgagg gactttagaa ctcattatat ttgctattat
      121 taaaaaaaat cataagagga tttattggta ttagtatgtt ttagtattta aaaagaatat
      181 tgaaaacttg aatatcaaaa cataagtaat acaaaattta gaatgaaagt cagattataa
      241 aaatgccagt tgtaatgcaa atactgaaaa cagcaccgat tttgaaatat aataatttct
      301 tgtgtatcat gtaaaactta tgcacttaca tgttaatgga catttctggt tttactttat
      361 ttggtcttgc acatgcagtt attgcacagt ttcatgtggc tctacagtat atgtatgtgc
      421 gctccactcc gtgtctgttg tgtgtaggtt tgtgtgtcag atttgccatc gcggtttgtt
      481 gccgctgggt tgtgtgtacc caagttgcgc cagtggtagc gagcaacatc tgtttcgggg
      541 ttgttgttcc tcttggctgc ttttcaattg gatttcctca agcagggcca aaagctgtca
      601 cctctgccag gctctgttag caatcaccgc cggcccctat attaagtagg tggttcaggg
      661 ctaagctaat aactctccct cctctcctga gccatcacac accagtactg gagttgttgc
      721 cggcctcagt gtgtgcctgt ttgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      781 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gt
//
LOCUS       KCVB01000134            1647 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6107pri ultra-conserved element locus
            uce-1299, sequence.
ACCESSION   KCVB01000134
VERSION     KCVB01000134.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6107pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1647
                     /note="ultra-conserved element locus uce-1299"
BASE COUNT          487 a          375 c          329 g          456 t
ORIGIN      
        1 ggcctgtcag tataaacata ttaataatct gacagcttca tctgcttttg agggccagac
       61 ttgacagctt cacaatggcc tgcgctgtag tccttctcgt acatgtctag ctttcctgtc
      121 tgaacaacac agagagttta ttaagacatc acactcctgt gaagtattga ttttacacca
      181 gtctctgtca cttactgtat taggccaagc aggagctcca aaataaacta tgacagctgc
      241 atgtaacctg ctgaatatgt ttgagagaac tcaggtaaat tcgcttttta ttttgctgta
      301 cgtctttaat ctaatatcgc ctgaccaagt tcggtgatca aacataaata cacatcagaa
      361 aaatgctttg attttttttt attttttttt tgtaaagaca aaattcagat tttttgacag
      421 tgacaaataa aggagaaaca cattagagag gagattgttg caattaagtt tctgcagctc
      481 aggaaaagga gcgaatgctg tcttgaacaa aggaggggga tgtgcatctg cctgtggaaa
      541 tgcgccccgc ctctcagaca gtgggtctgt ccgtggagag acagagggag agaggggtca
      601 gagggagcac aggtaattga ggtgtcccct cccctccctt ttttccaggg cttctcaccc
      661 catcctttca cccccccacc ctatccacac agcgggcaca attacgctcc ccatcacccc
      721 accagctggc ccccgtgatc ccacacttct tactaaacgc gactccatcg gccctttccc
      781 tcttaacctc tcagcaccgg ctccagggga atggtaaggc tgcacacaaa actgggggaa
      841 aaagaactca gacagttcta ctacttttaa cactttgcaa aaaaaaacaa caacaaaaaa
      901 aacaaaacag agaagttgag ttaaatgcag gttgtgcatt gagaattaaa ccggttttat
      961 ggcaggtgta ctgaggcatc tcagagtggg aacatacagc agctctgagc acaaataaac
     1021 cgcatgtacg actctattaa atttaggctg cagataagtt atacaaaaat attgtcatac
     1081 aagataacaa gacgctgcca atgttgtgat aattaatttt acactttaaa ttagaagaaa
     1141 tctgacacat ttagagagat ttaaaggaaa taaattctag tttcaacaag gaatagtctg
     1201 tccaacatct acagtagtgc taacaattta aatcaaactg ttatagctat caacatgtat
     1261 gttaattgag tggcttgaaa tagatttcaa cacccgcgcg tcagtctgat catatttctc
     1321 gaacacgctc cgagtgtctc ttgatagaca gtgttttcac tccatccctt cacattaatg
     1381 agtgggaagg ctctcctgtg cgttattacc tgaaataata ccatatggta aaatgtaaac
     1441 aacatctgca tattcatgtc ataacttcat ctgtttaatt agttccagca aagcagttta
     1501 ccctctgagg ccctccctgt cagagcagtg atggtagagc cgtgccctgc tgtcggtcct
     1561 gacagtcctc cattgttcag ttgacgctcc tgccctgtct gtttaatgcc aggtgcatct
     1621 ttacaggcca cggatcaggt gaagcga
//
LOCUS       KCVB01000135            1686 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6171pri ultra-conserved element locus
            uce-157, sequence.
ACCESSION   KCVB01000135
VERSION     KCVB01000135.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6171pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1686
                     /note="ultra-conserved element locus uce-157"
BASE COUNT          457 a          321 c          404 g          504 t
ORIGIN      
        1 tttatcagtt gtagtgctat tccacacaaa tttaaaccct gtcatctgct gatttttagc
       61 agcctggctt ggaattcctc tcaatataag aaaatggtat ggcccctgag tgctactgca
      121 gttttattac ctcaggtggt ttgtctgaat atagtttacc acatgtctca ttcaaaggga
      181 atggcaggta tgtgtcacta gtcataaatt gtaacttgcc ttcagcagta atcagttgcc
      241 aaggaaacct caaatgggat tatgaccttc tgttgtgtat aggcaagtaa actgttagag
      301 ttgtctacat ccaagagaca cacattaaag atttatgtca gtaaacaatt tattttctca
      361 tcatactaaa ttaatgcgtt gtgtcttgtg tgagaaataa aagtttatac aatttttttt
      421 tctatcaagg gtttgtattt gaagcaatat gagcattttt tttctctaaa taagacatca
      481 ctgtggggtg agtgatgtaa tcagtggggg ctgtgtagcc atatagtcaa gaagaggcat
      541 tagaaaacaa agaaagaggt agcgggagca gagtgcctgc aaggagggca gagtgttggg
      601 agggcggaga tagagaaatg ggggacggtg gtatttagta tgcacggcca agctgcccca
      661 tctgcttagc gcagcagtcc ctgtcgtata actgtctaca ggccgtgggc cacataaaga
      721 atgtctcaga tttgatgtaa ttccgcaaaa ttaatgacag ctttactcgg tatgggccgt
      781 gagggaatga ttagtgtttg atcgtccatc tgatcagttc ccttggctgg ctgcaggaga
      841 gatgctccac tatatgccgg catgctgcct atggcaagca catacgcgca cacacagata
      901 cacctatccc aatatcatta cttggccccc atgctctagt tgaggtttga attctggttc
      961 ccgttaccct caacacagac aaccaatatc ttgttaaact ctctatgctg tgatacttca
     1021 catcaaaatg aacacctact gatgggctgg atgcaggtcc cactggtggt gtgattgtgt
     1081 acagggcagt ttttatttca ggctgtgcag tatttcaaag gaaagtgggc caaccagcat
     1141 ccaatttaga gaactccaag gtcacactat gaccttttac aagatatgct ttactgcagt
     1201 tgtgattgga attgggtatt agtttttaaa agagattggg aatggattgg gaatttgagt
     1261 gaagaaattg atccactgga tggattgcag gagttggtca agtgaaattc ttctcctcct
     1321 tttaattcag ccccacctca gccggcccaa cccccatctc tttcttcctc ttgggctgct
     1381 gcgtttacaa gcgaaggggg cattctgttt tacaagtgag cctggtgcgt cttggtgtgc
     1441 tagcaacatt aaaattgaac ccgtcttatc tgtgcaaagc gtgaggcttt acatcagagg
     1501 ctgtcatgct gctgccatga ggctcctgtg tgcttttatg taatgtggtg cagtcaaaaa
     1561 cccagtggac aattaaagtg agctgataga taaacaagtg attgaaaaca gggatgttag
     1621 tttgtggaat gtaaggtaga attggaggaa ggcagcttca ctgctgaatt gattattatt
     1681 attggc
//
LOCUS       KCVB01000136            1436 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6211pri ultra-conserved element locus
            uce-951, sequence.
ACCESSION   KCVB01000136
VERSION     KCVB01000136.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6211pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1436
                     /note="ultra-conserved element locus uce-951"
BASE COUNT          390 a          301 c          288 g          457 t
ORIGIN      
        1 ttgttgatga atatagtaga ttctttaaaa tattttactc caaagttttt ccattaagtg
       61 tttttggaaa aaaagaaatg caacattttg taaacaaact cacttacata ctgtgacatt
      121 gagaaaaatg tgtctctaca tcttatagtg tttttcttga cgtcatcctt taacctaacc
      181 gcaaggtgct aaatttggtt tccaatgttt ttgttccata attccatcat ccctgtgttg
      241 cagtcatgct actggcctaa gcagtctcct cagtgagcga ttttcttccc tgtcatgaac
      301 actagtgggg aaaaaaagcc aaatcgacag cacagctctg gttaaagtga ggatggcgac
      361 accctgttta acgacggcat gtactttgtg ctgtaagtgt aacacgtgat ttgcagtaca
      421 gtagatttta ttttaaggga tccaactgcc ttcttgggtg atgatgaagt agcagcgcgg
      481 ggatatagga agttggacag tctctggagg ctttgaccag acatgttttc ctctttcttt
      541 agcccacaat tagaagcaat agtctcccac ttctacgatg gctccctctc ctcagcagag
      601 atatatttat ctgaggataa ttggccatta aatcctcact tgacaactgt gaaaatgcca
      661 ccccaagagt aatctggctg ccagaaaatg acaacggttg gcagagctgt cgactgctgg
      721 gagccacatc aggaatggat ggatctattc cacacactca catccataca cgtgcacacg
      781 tacacatcga acagagctgg ctgtcctaat caaattctgt cttgttcgca ggcttgtgat
      841 tttattttat tttatttttt tagaaaaagg aaacccctca ttatgatcct tattaaaaga
      901 caggtctcag cactggggat aaaacaccat cctttaaacc cttctgtcac tgaagagaaa
      961 tgcacttttt ttagctttat ttattttcat taactttgca agcaccctgc tttgatcact
     1021 ttatcagggt actctatatt caattacgga tgtcgcactt ctttgaaagc tcttatgtaa
     1081 aattggattt ttctcagcat agcgtctgtc tgacttgagc tgatccttat catatggttc
     1141 aaagtatctg ggcttagcag ctttttgagg ctgtcgaaag cctgaccaag gcaaatgaga
     1201 agacaagcgt tgagctaaaa atttctgtcc ttttcgtcgg ggtgcctggg ctgtctgtca
     1261 ggctgctaac atgaatcagg ctgagcagca gatacaggga gtgtatctat cctcaaagta
     1321 cggagagaag accacgtctt tgatagtgtt tgaaatactg gtcattatta gccccttggt
     1381 gaacatcttc ctgtataggt atcttctgct tttctctctg ctcaaatcca tataag
//
LOCUS       KCVB01000137            1811 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6253pri ultra-conserved element locus
            uce-112, sequence.
ACCESSION   KCVB01000137
VERSION     KCVB01000137.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6253pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1811
                     /note="ultra-conserved element locus uce-112"
BASE COUNT          537 a          379 c          382 g          513 t
ORIGIN      
        1 atcataccct ggcctaaaag caggctaagg gcttttagaa gacacacaca gaatggactc
       61 tttaattact gactcaagat cctgcagtag attaaaaaga acctcgcgga agcttatttt
      121 catatccgca gaaattttca atgaatataa aaatggtcat tgattatgcc gttaagggag
      181 ggaagttaag aagggaaata taaagtacat ataatcacat taacttgcac tgattaatgt
      241 aataaaatgt gatctttttt ccccatctgt actgaacaca ttcagcactg aggagacatg
      301 ctttaaatac taaagagcag ttctttaaaa gccacttaac tactctgctt tcaaagacat
      361 ctgcgaaagc aatgtagata actcatataa ctccttctac agtgaagtta gagaaagtgc
      421 atctgtatgg atgaataagt gagaaaaaaa atcaaaaaca atgactcatg tctgcttctg
      481 atgtctgtgc atttgcagta ttttcagcac aaatgactat atgtgtgtct ccgctcccag
      541 acacacaatc aaccttcctg catgatcaat accaaagcat ttctcaccat tgattataca
      601 attatagcga agttaaatct gtgtcacact catgtagtag cattaaccga ttcagccagc
      661 agtggagact aagaggaggc aagaggcttc ctcttcactc tctgtttttt tttctttttt
      721 taaaaaacgt ggtgcttaaa gtgagctcag tgtagtccag gtacttcact cagatgatta
      781 taatgccacg agggtgcaat acatcactct tattagccac actggagcac aatggtatca
      841 taattaatgg tcgcagcagg attgaacgct gccctgacat ggaaaagtgg gcctgcttaa
      901 gctctacaag gcatttaaat atgcagagtg aatggcacaa cccaaaccct cctgctctaa
      961 ggacagccca cgctcattag cctgacacct tccgtggcat tgatacaagg acgaccggct
     1021 atactcacac acgtacacac aacacaaatc acaagcacat actcaaaaac agtgcaaacg
     1081 gtccacagcc accaaatgta aagttgcatg gtgatatatg aaaagagcaa tcttgtccct
     1141 catcagtgcc catcacctct tttgcatttt ctccctgtat tgttgttttt gtcttaaggc
     1201 ttagtctaag ctgaatgcag atggacatat atgtgtcagc agccattatg caaatgtttg
     1261 gtaatggcgt ttgggctgtg tgtgtgtgtg tgtgtgtgtg agagtgtgtt tgcagcgaat
     1321 gtgcgtgctg tggcagaatg tggtgtctag tggctgtggt acggtgtcta gcacagggaa
     1381 actgcctgcg cggcgggaga acaatgcaca taaaagactg tcggacgaag cgtctggagc
     1441 tgacgtcttt cacatggctc tgaatggagc agcagcaaag ggtgtgtgtg agtatgtggt
     1501 ttatatgctt gtgagtgaga cagagagaaa aaaataagag aaaaaaataa gaagaaaaca
     1561 ataagagtgt gtgtccccat ctcagtcatg tgtagtaaaa tgcatgttat tttatgtgca
     1621 tgaaaccctg tgctcctaaa tgcatgtgca gggtggttaa gtcctctaag atcgctgtat
     1681 gatttggatg tttctgtcct tcacccaaca cacactctcc tcctcctcct cctctcctct
     1741 cctctcctct ctgtatttgt gtgcttgagc aaagtctatt cttatccctt gcccactgtt
     1801 ttgtgtaaca a
//
LOCUS       KCVB01000138            1602 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6330pri ultra-conserved element locus
            uce-1165, sequence.
ACCESSION   KCVB01000138
VERSION     KCVB01000138.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6330pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1602
                     /note="ultra-conserved element locus uce-1165"
BASE COUNT          465 a          353 c          339 g          445 t
ORIGIN      
        1 ctccaagatt aagtgcgctg tttcaggttt tcattcccag gggctctgct ggctgatgtt
       61 gtattgattg caaaagttta ccagtaagta tcacaaacaa caatagccag gtcaacgatc
      121 ggtgatgcct gaaccgctat tccccggcgg ggggctacac gttcagggct aaagcactgc
      181 catcatcata gctgtgtgct gggtgcagag agggctctcc tgagctgggg gaagagaagt
      241 gaactggctt ttcctactga catttaggaa agcgatatgt cagtgtgaaa tgagcgggcg
      301 tgagataatt ataagccttt gcattataaa aggctgcatc gacaaactgt cggattggcc
      361 ttccagagag gcactcggcc ctttcagtta ctcctgcatg ggctactctt tatcccattt
      421 aatggtttgg taattttttt taacatctcc tgaaaagacc cgtctctcta cacacacata
      481 tatatgacag atcaaagctt cgagtgtaag gtaaatgatc cacagtacag tctatttcca
      541 aatgaatcac tttccatgtt tatgatagca tgtccaagag aagaagtgtt accattaagc
      601 aatattttct caaagaaaac tgcagtgcta cactctagca taagtgaaag ggctgagcag
      661 taaagaacac gattcaccct aaggccatct ccggcaggct gaatgatggt gcatccttgg
      721 ccctgcatgg aggacagcac acaaccctcc atcacagtgc caccggacca ttacccaggg
      781 actgcaacgg ccagccgcac caagcaggcc tcctccactg ccccatgaag ctaatcatgt
      841 gatttgatgc aatgacagag taatgacaca gattggtggt gtgttcagct tccccctgct
      901 ggagactaca gaggaggtgg tgaacacaca agactgcctc gagccagagc aagagccaga
      961 gataccacag ttgagagcaa gatgcagaag atatccaagt actctcacaa gtgaaatcaa
     1021 gattaatggt actgccgagg gattctttta tgtccttgag taactatatt tcacttaatg
     1081 agggacatta gccgtaataa aactactcag aagtacaagc cgttaattaa tgcctccaca
     1141 ttaccagtga atgagaataa ctgagttgta ggacccattg gagcaaatat taaaggtgca
     1201 atctgtgggc acataatttc cactgctctg tgctaattaa tcatttaaga tatatcctgg
     1261 tactaaattt ctttaccagt attttgaaac tttgattact gtacagcaca tcattcctta
     1321 ttagattaag tctctagcaa ggttttaaat tactgtattt gtttacctct tattattatg
     1381 gatggttgca gtgaaggtaa ttactgccag gctgccttaa agatgaggac ttgtgtgtga
     1441 acttgatggt aagtaatctt aacagttaaa ataaatatca gctccaaaat cctgtttcac
     1501 tctctttcgg tcttatgtga ttgttctttc atcaggcgtt ctaaaaatta cttccagatc
     1561 tcgcctactt caacatcaca gaaatacatc tcactaacct cc
//
LOCUS       KCVB01000139            1665 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6433pri ultra-conserved element locus
            uce-546, sequence.
ACCESSION   KCVB01000139
VERSION     KCVB01000139.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6433pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1665
                     /note="ultra-conserved element locus uce-546"
BASE COUNT          464 a          346 c          405 g          450 t
ORIGIN      
        1 tatgcttcac ttctaaaata ttaccttttt tctcggttga gagcctgaac actgctgcac
       61 tcagaggtgt ctcctaccac cttacagcca ctaaaaccac tatctgtttc atcaaaagtg
      121 gtggctggtc ggcaagcgga gcgatgaaat gtattaggag ttgctagcga ggtgtgactg
      181 aggcagggag tggagcttta caggagaaag cctctctttt ttccttggtg cagcagcagc
      241 agcagcagct aggtgatgtt cctccctaga ggctttccct gtcagaacag agtcagaggt
      301 cggggtaagg gtggaggttg aaaggactgg gctggctgtc accttcatca gcacacagag
      361 ggggaggcca cagagtagta acagaaactg acagatggga gggaaagagg cacaataaag
      421 aatgagcagc gcagttcaaa aggcttgata ttacagtgaa gggagactag agaaaatcag
      481 ctgaaactac tacaccattt tacctgaggc tagtcagtga gttgtttgtt ttgcctctgg
      541 aaaaaacaga aatattcatt ttcttatcgg aacatggtaa ctgacattta tagaggtgtc
      601 cagtgagcag catgagctat actggcagat agacagggag agcagagggt ggggcgggag
      661 gttggcgtgg ctggtggtct gttgggctgg ctgacatctc tgtctcatcc tctctcccgg
      721 cagttcatgg catcaagtgg acttgcagca atgggaacag cagctctggc ttctcagtgg
      781 agcagctagt gcaacagatt ctggacagcc accaaactaa gccacctccc cggactcaca
      841 actgcctctg cacgggcact ctgggtaagg gctagtggga gggatggagt ttgttctcaa
      901 gaaagaactg gaaaattcag cgagagaaac aaaaaggcca taaagataat gttttttata
      961 tggtagatct gagaaggtgg ttgaggtcta ttgggtattg atttatagtg aaattgactt
     1021 ttggggttat tggcttatta tttttgttta ctgataactg tttcagagcc ttatttgaaa
     1081 tagctcagct gttctcggat gttatgttag tcaatcaaat attttcacat gtgcaattca
     1141 ctatgggctg tctctcagga aaagaatgta gagtaatggt ttactaattt catacacaaa
     1201 ttactctgga taacccttag cagaatattt atttctgatg acttaaggta acaagccatg
     1261 caaaactttc cttatgcttc cctctctgaa tacttaaaag aaaggcttgt ttaatcgtcc
     1321 ccaggagaaa atcgtctttc acaaacaata aaatatgaaa acaaacagac cattcatctt
     1381 tgtccctgct ccttgaacag tagcattgtc caatttttat tattcctaat atagaaatca
     1441 acagtgtttt tgtcttttct tttttgccac ccatccaaat ccccaacctc ttttgctctg
     1501 tttgtcctca tataggtgca gggagcagtg tgcaccacaa atgcaacagc gccaaacacc
     1561 gcatcatctc ccctaaggtg gatccccgct caggaggcta cagcagtgct cactctgagg
     1621 tacaaaacaa cgatgtgtca gaggggaaga ccgagcacag tgccc
//
LOCUS       KCVB01000140            1903 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6476pri ultra-conserved element locus
            uce-197, sequence.
ACCESSION   KCVB01000140
VERSION     KCVB01000140.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6476pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1903
                     /note="ultra-conserved element locus uce-197"
BASE COUNT          576 a          440 c          385 g          502 t
ORIGIN      
        1 aggaggagga ggagggggag gagggggaca caatgtgtga ggacacaatg gactcctcat
       61 taaccagtga tttatcatga taggatgccc agttcaacct tttgcctccc ccccatgtgt
      121 gtgcacatac acacacacac ctctagaaaa ggatcctatt gttggaaaaa gaagagagga
      181 ctgtgaaagt ggagttgaaa gtgaaatgat atagttaaaa agtcaaatga aaagtaaaag
      241 caaaagaaaa tcatccaaag ctgaagtgtg tccttttgga gagtgtgtga gaataaagac
      301 cgaagggccg acacgctcaa acttctgtgc ttttcagttt gggttcagtt cagtttaaca
      361 gtgaaactat taaaaaagaa gctgttagct tctcctacat ggttattgtc actttgattt
      421 catttggctt ctctcttaga tttaaaacat ttgactataa aagactttaa tgcagttttt
      481 gacatctcag attgctgaat ttccctctcg taccagtttg tgagaaaagc cagcgccccg
      541 aaaagtcaac tttattaaac attacacaca atctgattca caccgtaaaa attagccttg
      601 caatagacac accctccctt tcctgagcca ggctttatga aagtcaaatc tctcggtcat
      661 gatgatatca tacatgtcag aaagcctcta aagatgctcc atgcatgcat atgcagggag
      721 atcatagtcc atcctcagcc gctctgccct cacagaggcg gcgcctcctc ttgcacataa
      781 aggctgtata atgagcgttt aatgtatggc ttctcaaaat gcagagaaaa agggaatatt
      841 caacgtggcc catgtggtgg ttttgtaaag ctgctgcacc aaggaacaca ccccttttca
      901 ctttctcctc tccatccttt ccatgcaaac cccccctcct ctccatccct ccctcccttt
      961 ctgaatccat ttgcttaaat caatctcaca ggaggcggtc gtggataatg taccagccag
     1021 ggtttgtaaa ccgttttgtt aacaatgcat tttgaaaaga aaagaaaaga ggaggagagg
     1081 agagagataa agcgagacag agagagtgag agaaggagag agggagaaac gggcagaagg
     1141 gtgctgaaag aggaacagtt gggtgggtgt ggtgtgcagc gctgtttcgg acaggccctg
     1201 tcgactggtg acacccctgt cccatctcct cctccctcag ctaaagcctg catccgccca
     1261 attcggtcca gtcctcgctt tagtcacacc acctgccttc actattcttc accacacacc
     1321 cacccgtccg ctgacaggac aaacagacgg tataaacccc cgtaactcag cctaaacgcc
     1381 tctacacaca cacactcata cacccacaca tacatgctgc acaagtagac acactgataa
     1441 agctagtgtt cccttgatag gattataata atcaataatg agtaatgaga gatgatagcg
     1501 gccaatttct caatcacatc aacattcagc ttagtggtga cagagggaca catgtgactt
     1561 ctctcacctc ggttatgtag gaatcataca acactacatt tgtacaattt gtttctccct
     1621 ttacttgtaa cttttaataa aagagtcata atttataagg ctttgctact aaactgccaa
     1681 gacttcactt cctcagttga aatcaaagct aataaccatg acacctgctc aggacagacc
     1741 agctaaaaag ttccatatga cattttcttc aaagaaagaa taaatgtatg agagccgcag
     1801 tctttgtaac agttttctgt tgtttattgt gtgttttctg tttaacatta atcatggcag
     1861 tcccagatgt tggactttcc aaaccaattt tataactgaa gac
//
LOCUS       KCVB01000141            1363 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6519pri ultra-conserved element locus
            uce-503, sequence.
ACCESSION   KCVB01000141
VERSION     KCVB01000141.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6519pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1363
                     /note="ultra-conserved element locus uce-503"
BASE COUNT          461 a          247 c          299 g          356 t
ORIGIN      
        1 ttagaagttt attggtatga aataatcagc tatcaggcac tttggttaca tgtgtatcac
       61 actggaactc gtaaacaagc acacaggtat gtgtgttgga acttataaat tttaattaac
      121 cctttaaaaa attcaatgtt cttggtcctt tagaagaatg ttgaacacca ttgaagaatt
      181 gtttgaggaa gtgttttttt atgtatatat taaagtgaca aattttccat ctcataaatt
      241 tttaaacgga ccaatcagag acagagaaag gaaagacatg aagaacacag aagagaaaga
      301 gaaagctgca aaagttacag aaagctttac ggtgagatga gtgtgcgtag tttagcttag
      361 caccactgac ctctagaaga acccccacca aggtcaaaag gtgggagtca gagcacagca
      421 gaaaatttct cactgaattc atttccaact ctttgattta gctcaataaa tttcaactgt
      481 ggtcagatca gtgctacttt tgtctcatgc aaacttaagg gaacactgca cagcttgcca
      541 attttgaggc taataaccag ataaaactgg accgatgtgg tggagtccag ttgaagagag
      601 aaagatatgt tgcatttcca tacaaattgt ttggagacac gaagtcaagg cttacccaag
      661 tctggcttta gctcagtagg caagcttaat ttccgtgaca tctttgctgg aaaaaagaga
      721 acatccctct ttacaaacac acgtctaagc taacacacga tgccatgcaa agctaggtaa
      781 ctagcactgg gctaggactg tgcacaaagc taaaatacac caaaaacaac aaaaggctgt
      841 agaacgagtg ctggtggggt gtggggcgtt taagtaactt caggagccaa aatgctttac
      901 acaggagaag gcattgcaga agaacttgga gacaaccctg tatatcacag aggcatgctg
      961 ggaaagacga aacataagag gtgatctctg actgcacatt gctctgaaag agaggactgg
     1021 tatagtaaga cacatcaaaa ctgcacagcc atccaggacc ttcatgccac aggagcatgc
     1081 agcaagaaca agaaggtaac aagaaacaga aaatactgaa caggagatgg aagaagacat
     1141 tggaaaagat ggtcaaaagt tcctgaggtt gtccacggct agttagttaa attatctttt
     1201 ttcttctgtt gttaataact gtattaggca cacttaccag aaactgtctc aagtagtaga
     1261 aattacactt gattactgta tgtgttagca cggttcggtt taaatagatg ctgtttgcac
     1321 ccagagtgtt tgtttcgtaa cccttactaa gtagtttggt gcc
//
LOCUS       KCVB01000142            1610 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6559pri ultra-conserved element locus
            uce-1259, sequence.
ACCESSION   KCVB01000142
VERSION     KCVB01000142.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6559pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1610
                     /note="ultra-conserved element locus uce-1259"
BASE COUNT          474 a          343 c          336 g          457 t
ORIGIN      
        1 aagacaaaat ggccatcaga gaagactctg ttctttgaga ctaaaagaaa cggtggcgtc
       61 tctgttaaac acaactgtta tgacagagat tgataagtaa cttttttttt ttgatttggt
      121 ggtgagatga aactcatcaa attgcaaaat gaatacccac aagcatctgc aaaaataatg
      181 gacacaacat aacactattt gtgacttctg tactagcatg atattagttt ttctgttaag
      241 aagctaacta taaacacttg atcttttgtt gctaacaaag taaacaagtg agcacatgaa
      301 atcccatgaa atctgactgc cttgaattat aaaaacagcc atcgtcatca gccatcaacc
      361 cccgcaaggc cttcatgaaa catgacattc aaatatgtgt actctggtat tgatggtatg
      421 taaagccatt cacagcctga gagcagaacg actgttactt tgtaaaatga agtgaggtct
      481 attaaaggaa gtgtattata taaatgtgtg aaccgtgttg ttgtgacatt attagaggag
      541 gaccatcctg tcagcgagac ccgtacaaca aagaacacag attcacagtg aagcactgat
      601 gggcacttta acaaagaatg tgcacatcat gtgagagtca cactccagca ggtcatacaa
      661 tacagtctcc cccctctctc tctctctctc tctctcactc tctcactttc tctctctctc
      721 tctctgccac ttgcagagtc tatagaacca tctcccgtgc ttcagggctt ttcaatgtag
      781 ttattcaagc agaacacaac tgtgtgcctc catctgatag aaggttaagg acctcttcac
      841 tgctcacaaa aaagcccgcc acacagaggt gaggccaggc tcacctcagg gaagaaatct
      901 ttctctgtcc tcctcctcct tctctttcac tcttcctttt ctcctggctt ggtgaaaaga
      961 aaggttcaaa ggataatggc tgtcttatca ctatgtaaaa ggggaatggg aactctagtg
     1021 aagtgcctgg ccttttgtga caatgaagaa aagagcaaaa gtctatttca ctgtggtata
     1081 tgcactggaa cagtccatga ccacttaaaa agaggaggct gttggaagac agtcgggggg
     1141 gaagaaggcc gggcagctct ttagctaaac ctgtagcaaa cacacctgaa ctactctaac
     1201 ctcctccaaa aaataagctg acaggagtat attaggtgtg tgaaatgttt gcttaccaga
     1261 aatttccatt ttattcataa ttcagaatgt agggttaagg ttatgttgtc aacctcctgt
     1321 acgacagtag cagcagtggt gacaacagta gtaacagtga tattggctga agaggtaata
     1381 gtggcggtac cgtttcaaac ttctctgtac tggcacacag tgaatgtgtg gaagcagctg
     1441 cagccttgtt gcctttacag ctggtttgat aggctggtag agttcagttc ctgcagtaca
     1501 tcatgcagct ttggaaggtt tacaggctgc actgagtttt tgcaaaatca gagtttgtct
     1561 tattctcttt tctaagctaa cagctgacca tactttggtt cagtcctggt
//
LOCUS       KCVB01000143            1616 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6597pri ultra-conserved element locus
            uce-333, sequence.
ACCESSION   KCVB01000143
VERSION     KCVB01000143.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6597pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1616
                     /note="ultra-conserved element locus uce-333"
BASE COUNT          478 a          343 c          351 g          444 t
ORIGIN      
        1 tgtttgattg gttcattgtg aaattatcat cagatttgtg ttgtatttgt gttttgctaa
       61 ttaaagacac aacaatgcaa ctcctgtaaa ccacactgcc cttataaata atacaaaaaa
      121 ggaaaatatg gtttgtgcca aggtgcattc gagctgcaac acactttcta ataccagaag
      181 taatcttgat taatcaaagg tactgtgttt tccagacatt tttatggcat atctagaggt
      241 ggtttgcttt tggataccac tgtaataaag aaaatgctta tgcacgaagg ctgtgtgcct
      301 tagacagcta tgcaccctca tgtagagtgc ctctgaaaat taggagctgt aatgaaaatg
      361 gataataaag tctaccctga aactgtaatg tggcatttct gtttattttt aatccataca
      421 ttcaaagttg aggactgttt tcatcacagg ttaattagtc ctattatgca gtgcagaggc
      481 agcagcagta ccgtcatcta ccggatattt tctgtgtgca tgaccaaaac gcttctcaaa
      541 tgagtgctcg acgctaccca cccaccccct gtgacaaaat ggcctacagg cctcatctgc
      601 gcattcaaag tcacatagca cagtctgcaa tcacataaaa aaaaatttct tggtgtgata
      661 acgtttgcgt tttgtgtctc agagccgcgc gtaaacgtgt tcccattaag agaatgtctg
      721 tgttaatgta actctgctct cttatgcatg acgcaaatag taagggatca gcggtaaacg
      781 aggccacgca ttgtctgccc cccgccctcc ctctgcttgt caggaagcaa gctgattggc
      841 cgaggcctag caaagggtgc ccggggatcc aaaactgcag gccgcactta gcttaccgtc
      901 aaaaataaac caaaacaata acagactaaa ctgagaaggg ggagaaacac cgatagagtc
      961 tgaagacgac gttatcaggt aaaattattt tgaatctgtt agaaaggcat atggaataac
     1021 acgcaagggc ccagtcggcc gtagtgggcc tctttcagcc tcttcgaaaa acaacatgga
     1081 agataatgcg gcacaggcag cttcacttag cttagcaagc tagctagccg aacagacggc
     1141 tgtcatcgct catattatgg tgacacgcaa aaaaataatc tcatggagca aattgggtct
     1201 gttgcgtatg ctttcgactt tgtaaatgct gtaaggccgt gtgaagttag cacttaaatg
     1261 gtcaaataat attggttgtt acatttcttg agttagccat tatgctaaaa caaaatggcg
     1321 ctcagaggaa cagccctagc ttgcttgcta ggttcgttag ctccggtggg tggagggcgg
     1381 taaccgccct agtatccatc gcataacatt tgcggtactt tcattggcgg ccataaactc
     1441 tgagcatgtg tagttttctg caaagagttc aaaatactat gtttttaaaa cgataacgtt
     1501 tgcaacacgg tgttactttt atctaaaaat gatatatccg tggaaaccag ggacccacaa
     1561 cggcgtccca aaccacaacg gcactgcaga tatgccatat tgggcttatt gtaaac
//
LOCUS       KCVB01000144            1577 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6634pri ultra-conserved element locus
            uce-376, sequence.
ACCESSION   KCVB01000144
VERSION     KCVB01000144.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6634pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1577
                     /note="ultra-conserved element locus uce-376"
BASE COUNT          424 a          309 c          309 g          535 t
ORIGIN      
        1 ttatgttttt cacatgtgtt tcacttcggt cactcagttc atgaaggcat caaatgtcat
       61 tagatatgat ttgcaggcaa acatgcatgt attgactcaa tttcaacaca ggaaccacag
      121 caaattactt atttctgaca aggaattaaa gtgacaaagt gcagaaaagc agcaaatcct
      181 acacagaagc accaagttta gaggaaacag gacttcagat gaggttttct gggcatctct
      241 ggcttatttt tactctgtat tacaataaaa atgtcacatt tttccatatt cactctagtt
      301 cacattgatt agctttttta ttttgttttt tattagaaat acaagttagt agcacgctgg
      361 tgactaagtg gaaaagtgtt ttaatgattt attttagcgt tattcgaagc agactttggg
      421 cagaagggct gttctgtgaa ttacgcggtg ccaaattacg cacaacaacc agcagtcata
      481 tcataacctc cagcttgtct ttaacttctc cttcatgagc tttcagcgca taaaacagtg
      541 tgttgtgtga gtgtgagctg ctgctggtgg ggcagcaccg ctttttctat cttgatctga
      601 actactaacc aagtcttaga gagatcaaaa ttcaatcccg ttctgagaaa aaaagagaaa
      661 tgacgttatt tgaatatgtg cccaaaatcc ttaatgaata acttaggagg caaatgctgc
      721 cccagctgtt tcctgttcaa aatgtctgtg taatgtagat aaatgtgagg gattttctct
      781 ctaaatccgt ctcctgttcg ctaaatctca cttctccaaa acgagcctgc gcaatggaac
      841 aaagtcggaa tattgagatg tgacattgcc cgcatctcat cctctttctc cccgttttta
      901 atgacttcaa acaagttcat tttcgccggg ctttgtcttg cggagacccg tggggatgtc
      961 tagcgctctc tcctttcagt ggcatttatg tgatgtcttc aaggtaactt acctgcattt
     1021 cctttgacac tatagattat caccttcact tgctcactat ttgcacagag catccttacc
     1081 tagtttaaac tgaaaaaata aaagggaatt tccatcgctt atttgtattt tgctgctttt
     1141 aaatgcatga ttcatgtgga tttattctga tttttatttt tattcagtta ttgagcaact
     1201 gtcttgcatg tcttaatcat tttaattcaa cagatattaa gagattgtca ttcttctgct
     1261 ttaattaacc ttttttttta atttaggaaa tcttgacatg aatcgtgcat tagtttcatt
     1321 actcggatga atgggttttc accctcagcg cttctgtgcg ccctgcgctc tttttcacca
     1381 gaacgtccta atgcagctct gcaactttca aagtaattac tgctgagtgt gcattattaa
     1441 gcgctaaata tctaacctga ggctgcgggg tgaagagaga ggggagctta acatgaaata
     1501 gcctacgtat gggcgtcggg accgtgcggt tggggttgtg atgtagatgg atagtgtccc
     1561 gtgtttattc tttttat
//
LOCUS       KCVB01000145            1367 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6673pri ultra-conserved element locus
            uce-1301, sequence.
ACCESSION   KCVB01000145
VERSION     KCVB01000145.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6673pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1367
                     /note="ultra-conserved element locus uce-1301"
BASE COUNT          511 a          210 c          231 g          415 t
ORIGIN      
        1 ataacatttt taagttacaa cagatttctt agataattat ttaattaaaa agaattgccc
       61 aggtcacgtt ttaattatac ggttcaatgc cataaatcgt tagattgttt gagtgaagaa
      121 tcttaaaaca agcacattaa tgcctgaatt tatattttgt atatgtgacc gctgcaagga
      181 agttctctgc ttaatggttt aataacataa tgtgtattta accaggggtg tatttacact
      241 tacacttttt ttttgtttgt gtggttaaaa aaatatcata ccttcgctca gattttcata
      301 gatgcacata tttcaatgta agtattaaac taattgaatt acgttatttg ttcataggat
      361 ttggaaaata gtttccagtg tagtgagaca attctgatta aatattgaaa cactaattag
      421 gaaaggtagg cctgaaaaac tcatactgtg aattaaaaat aagtcatttg attaattttg
      481 ttgaaaagac attaagccag agagagagag agcagacgac agagagagat aaagagtcag
      541 gaaataaaag cccattacaa gcggtagatt ttattatggg ataacagcac ataaagcaca
      601 tctaaaatcg atgtgtccaa tgcactttta ataaaggtaa tattaaaggt acagtttcta
      661 agtacaatat aacaacattc atattagaat gaaaagtatt taaaagacaa cattaaattt
      721 cttatttttc tttacagctg tatttacaaa atgaatcaaa atacttattc tcatatttaa
      781 gaatcagaca agaaggagca aaactacgtt tgtgaataga ctacctgcta aagagagttc
      841 aaaagagctg gataaaaagg tacatgatca tctgtcccca ttttaacatc agtagcaaac
      901 gtgtgactta gaaatttgtg acaaccacac actagtttcg gctttcccat aatttctgct
      961 gcttttgcat ggaaaaaaaa aagaatccac ccgtgcttca cccacccttc atgttaagtt
     1021 accccggccg actgagcgta cattgaacaa gaacattaaa aaaatccaga tgtggtgtat
     1081 gcctcttttc caaacacata caaatacttg atatacaatg cattagtaaa tcttctgttc
     1141 ttggtgaaat aaaaaaaaaa gttgatataa aacagaaagt gaagaaatgt ggattgcctt
     1201 atcaaaaaaa aaaggaaaaa gaaaaagaaa caatgtgcag gtgaacttgt gttctattat
     1261 atgcgagcat ttatttgacc gttcaagatc aacctgaaac aaacttccca gttgtgtctc
     1321 tcaaataagg tgaggggtga catgtacact ctaaacggac catcgaa
//
LOCUS       KCVB01000146            1456 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6710pri ultra-conserved element locus
            uce-36, sequence.
ACCESSION   KCVB01000146
VERSION     KCVB01000146.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6710pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1456
                     /note="ultra-conserved element locus uce-36"
BASE COUNT          316 a          310 c          380 g          450 t
ORIGIN      
        1 aacactgctc attgtcagct gtacaaaaat ggtgtcatta atgttttgtt gaagcattaa
       61 ggccaattca catgcctgta tttggcattt tgagtcaaag aatatagtgg agctaatctg
      121 tgaccgtgcg gccatacagc tgtgacgctc acattgataa aatctgtgat gtagcataca
      181 tgcagcacat aatctaggtt aaagttgttt catttcctct cgctgacacc gtccattcat
      241 gcgctacagc atgcgtaagc attgattcat atgtgattgg tcacataatt cacagtctca
      301 gggggcattt catcgctgac agacaagcta atctaaaatc ccccaattta caaatgaaaa
      361 tcccaccatc caattgagct aatagaggga cttgaatggg ataataagat gtggtgtaat
      421 actgtgtaaa aagcctgtaa cgttgtgttg gtgtaagagc ctgtgtgact ggatgtttga
      481 ctgtttgcag tgattacagg ggcctgcggg cctggcggcg gctccgtggc tgggacaggg
      541 agcttctccc tgggcaagga gatacgcctg tctgcccctt ctgctggagc tacagggacc
      601 aggacgtcag gaggggagag tgctccctct gagctgcctc tctcgctctc tctctttcac
      661 tctctttctc cgtctgtctg tctctctccc cctctcattc ccggtctcac tgtgtctgta
      721 ctattgcagt gcttgcagat gtgcacaaac aagggaggat taaagcaaaa cgaatctaaa
      781 cagacaaatg gaattagagt gatgggtgtc ggtgggctgg ttcacagtga taaacataat
      841 gatgctctgt gtatgtgtgt gtgtgtgtat gagtgtgttc ctgtgtctag gggattatca
      901 gtggttaggt gtgtggtgtg tatggctggg tgactgcttc tgggtgtctt tgtgtgtttg
      961 cctatctgtc tatgtttttg taattatgtg tgtgtatatt tactcggtct ctacttgtgt
     1021 gtgtgataaa tttgttttct acattgcctc acaaaaacaa gtctctgcgc cctctgcacg
     1081 tgtgtgttta tgtgtttctc tgtgttttcc tgttggccgg ggtcatattt ccttcttgcc
     1141 tttcatagtg tctgttgtat ttgatgtatt tgtgtactgg agctgctact gaagcctgta
     1201 tgtctctact ggacatcaaa actcttcacc taatattgac acaggcacgg aaagatgttg
     1261 tgtgtgtgtg tagacttgtt ttcttgtggc aggggtctgt gtgtttgagc tctgaggagg
     1321 tcaggagtga ggaggagcca gcatggggcc ggcgatcaac agcctggggc tcccctgccg
     1381 aggggctctg ggattagggc taaaccctgc cctgccagat gggcacaaca catccccaac
     1441 atcgcacacc agcacc
//
LOCUS       KCVB01000147            1238 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6747pri ultra-conserved element locus
            uce-871, sequence.
ACCESSION   KCVB01000147
VERSION     KCVB01000147.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6747pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1238
                     /note="ultra-conserved element locus uce-871"
BASE COUNT          375 a          226 c          289 g          348 t
ORIGIN      
        1 gtacatacag gatgaaaatt gtgtggaaaa attctaaaat taagtaaaaa aggacttatt
       61 tttaccttga taatcatgct gaggtttcga gattatctat taagtagagt ttcctggttt
      121 ttcttacaac agcagcgata tgtaatgtaa atagagggca tatcttttga atattgctgc
      181 aaaggatcga ttcagtatct ttaaatccaa ggctcggcgt gacaaagagc cgcttaaagg
      241 taaggccgta aaaatgtgat atagcaaagg actgccactg actgagaggc tgagggagga
      301 cataaacaag acggatgccc gtccccttta atataacatg aagactgagc tggaaaaaat
      361 aaaacacccc attgtaatga aatgcattta ctgcagcaca ggcttgaaat caggcagaag
      421 tgagcacaaa tgtcaaaaga gctctcctgt tgagcagagc ggcagaatta agaggcctga
      481 tatctaatcc atttcctgtg aaaccggcgg tgtagaggac atcaaagaga gttaaagggg
      541 attcagtgag agctgctgga gtggccaggc agtgaaatat ggcacatttg gtttcttctc
      601 tcccccggca ctgggccttt agaataatta gagcctaatt ctgtctaatt aacggactca
      661 attactcctg aagaaagctc atcaaaaaga tggcgggaaa gcagggaaga gaatggtagc
      721 gttttttctt taattaatga actgagttga ataacggcca tggaattaaa catttccccc
      781 aatgtgtatg aattgcaaat tggagcatcg tttctttttt ttttttccga gacgggagcc
      841 caggggctaa cgctgctgtc aagtagcttt ctaaaaagtc aaagcgccga tgcagcacta
      901 acaaattact taagattacc atctttagca catgcggagg tcctaacgaa tagcgatgaa
      961 ttaatatgcc gtctgtgttc cagcgctccc attggctatg agtttcactg atcctaaaag
     1021 agccaataaa ccaggcacca gcgtgaactt tatggaacac ttttcttcct gtacaattgg
     1081 ttgtctgtcg attgcatatt tcacatcctt gagacgttga tataagtctt gttaaattac
     1141 tcagctgaga tagagctgtg catacacgag tgttttcagg ggttgaaaac tttttatgtg
     1201 ctatcagggc cgtctgtgtg tgtgtgtgtg tgtgtgtg
//
LOCUS       KCVB01000148             911 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6785pri ultra-conserved element locus
            uce-834, sequence.
ACCESSION   KCVB01000148
VERSION     KCVB01000148.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6785pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>911
                     /note="ultra-conserved element locus uce-834"
BASE COUNT          282 a          146 c          240 g          243 t
ORIGIN      
        1 aaaaaaaaaa taaataaaag gaaaatcaaa acagctccct tattggattt gggactcatt
       61 tagattcgcc aatggtggcc agctaatcat ttgatcctgt tagaaaaagc agtgtttgtg
      121 tgtttgtgtg taattctgcc aggaaagtta acacccgccg taaataggcc ggggctcagt
      181 gtcttgaatt gtgcacagtc gctgctgtgt ttgtttatgt gtgtgtgaat gtgtgagtgt
      241 gtgtgtgtgc gtgtgtgtgt gtgtcctcac tggctgtttt tttcctataa aagcaatttg
      301 ccccatgctc gggctaggca atgagcagat gctaccattg taaatgatga tgcgaggggc
      361 cataataaag agttgataaa cacagaatta ctgctgggcc gtaggcacgc agagaaaggg
      421 aggggagggg acgaggggtg aggagagaga gaaaaggaaa ggaggggtaa agagagaagg
      481 atagggagag acagagacac acaatcaatg agacggatag aaaacgtaaa cagagatgca
      541 caggtttcga tagtggtgat gtaggtaaca aggcgctgca ggtaaccagt tcagtgatcg
      601 acacatataa actggtttag aaaaaatgag taagatcatt taataatgat agttgttaga
      661 tgaagaaacc aaattttaac atgacagaaa tgcaccagaa ttttctgttg cacttttgtc
      721 accaagctct ttgtatcaag atgtgcaaca cgtaacaagt tttataaata ataaacagca
      781 tccgtggcta aagttaatct attacagtag gggacagcag ggtataaatt tgtggttgag
      841 tccttgttga tccgctgccc cctgcactct gtacattgtt tatggactct gtcgccttca
      901 tgagcccatt a
//
LOCUS       KCVB01000149            1595 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6820pri ultra-conserved element locus
            uce-73, sequence.
ACCESSION   KCVB01000149
VERSION     KCVB01000149.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6820pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1595
                     /note="ultra-conserved element locus uce-73"
BASE COUNT          494 a          324 c          319 g          458 t
ORIGIN      
        1 atgtgcatga cttgaaacat tttgtctttt gcactccttt ctttcttgcg ttctctttaa
       61 ttatgctgtc tacgttgtaa catctgcatt aagcctcttc atatatctta tcagaatcac
      121 gtcacatatc atcataatgc tttgactatg ctgtgtgaaa accaggctga aaaccatggg
      181 actcatttgc tacacttgaa agaaacttgg ttcagctctg cgtgaaaatc ccactcatct
      241 aaaatttgga gcagacacac actacagcag gtgattgaga ggcaggattt taggtcagcg
      301 gggtgctgga ttcaacaagg atataccttt ggaaacacaa tcaaatagag ctattacttt
      361 gtgtcagcaa catttcctgt gtgaagaaac ggtgctccag agatctgatc agtgtttcag
      421 cggcaccagt tttatactgt atgtggttca agtaaattac atacaatatg aaagggctcg
      481 gctcctgctg acggacctca aacaatctaa gagaaaacac tggtacatcg tccagtcgca
      541 tacacacaca catatatatg cacacagaaa ataaatttaa tgttgtcact tccttctaag
      601 ccgtcaatgt caagggagac cactggcaat tcaaagtaat cgggaagcca cgttacaaaa
      661 ataaatttgg ggcatgacac gctgttggaa aagaatagca atcagcaaat ttaatgcatt
      721 tctgatgtat atttaaggct ctgacactag gccacagatg tacatgtgtt gggtttctat
      781 tttgcaaaag atatggtcgg tgataaccta ttatgggaca tcgtcagcct cgccactgcc
      841 tcagcctcgg ataggaaaaa ttccatcact tgctctatcc tggagatgta atatcgaaac
      901 aaattatggc tggaattgaa aagtcacaag cttattccgt cttgatcaaa ccactgccaa
      961 gaggataagt gcagaagtgg agataccccc aaaatgcttt ttattgcatt atcatcattg
     1021 acatctacgg cactcgcagt tcccagcttt agaagtggac ataaatatat atttttcaag
     1081 ctgtaccgca cacacaggaa gtaacaaaag taccaagagt ctcggaaaac gtgcaattag
     1141 tttgttgttg ttttagtgca caaatggcaa aaaattcttg gtgtgtgtaa ctaataggat
     1201 tttgatgtgt acctttagta ccatatggtt ttcattcata cccaatagct taagaccaaa
     1261 cagatataaa acacaaagtc ttcaaagacc gcattcatag acttcagaga gagattctga
     1321 tcaatgcaac agtaactacc tcctctgcta tcagtcagtg tttaggtgtc tgaagtcaga
     1381 cccaacaaga aaacaagcca ttaatgtggc cttgctgcac agacaaatct tatttggtgg
     1441 gacatcgttt gtaagacgta atgatcacat ctcaaagtac ataacgtttg attggatggc
     1501 aggagtttgt tccggcggca gcaataataa aagaaggctc atcttggttt tatggtgtag
     1561 catattgaac catctgagtg ctgccttgct ctacg
//
LOCUS       KCVB01000150             814 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6874pri ultra-conserved element locus
            uce-195, sequence.
ACCESSION   KCVB01000150
VERSION     KCVB01000150.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 814)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6874pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>814
                     /note="ultra-conserved element locus uce-195"
BASE COUNT          172 a          171 c          181 g          290 t
ORIGIN      
        1 ttatgtgcag ttcatattaa atgagagtca tataaacaca caaagcctaa cagggcatgt
       61 ttagtcccgt ttattgagta atgtaatgct gagtttggac ttggttgacc tgcgtcggga
      121 tgtggtaagc ttatgaagtg gtcccttttt cttgcaccca ggattccaga tgaaagatga
      181 agagtttcag ctggattctg cagagagcaa aaacactctt ttaaccaaca tgtttactgg
      241 tactggtctt ttcataagct tcttattgtc cttgtcattt atttaccatg tgatacctcc
      301 ctgccagtta gattggaagt aggtgatgtg gaacaattgg gagcttaagc gaccaaccgc
      361 atatagaacc ggtccccttg tgtcatgtga ttttcatttc atttgtgtcc accgcattcg
      421 taaaaaagat cctcttgagt ccttctgcat atcgtccact tctgtttttt tctttgcctc
      481 cgttcctttc cttccttctc cttgtagttg cttgctttat gggagtcttt cttcagtatg
      541 tttttctgct tgagttgggt ctgtgtatgc agacacgtgc gtggcaggtg tgtctgtgtt
      601 atttgtgcgt gcttgtgtgt gtgtgtgtgt ctttgctttc cctttatagc cttctcatgt
      661 attcagctac ttacatcctc cctttccagg ttcttcaggt tcttttaacc tcccaaactc
      721 tggggacatg ttcttgagca tgcagagtct gaatggggat tcttaccaag gggcacaagt
      781 tggagccaat gtacagtcac aggtaggatc aaca
//
LOCUS       KCVB01000151            1422 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6918pri ultra-conserved element locus
            uce-501, sequence.
ACCESSION   KCVB01000151
VERSION     KCVB01000151.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6918pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1422
                     /note="ultra-conserved element locus uce-501"
BASE COUNT          392 a          327 c          305 g          398 t
ORIGIN      
        1 aaacatcaat tccactcgat atcccgcaaa cccccgtgct tctcaacaaa cgccagaaca
       61 aagaagacgc tatgtaaact actgccgcgc tctgattagc tctcattcca gagcactgca
      121 gaaataaggc actcattccc caccctgttt gagataactg ccccctttca gcagccacat
      181 catccctcca tacccataca tgctttgtaa catgagtgcg cctgtttagc tattctcatc
      241 agctgcttat gtagtgacat taaggaagcc tattgcttac agaaccaata atctatatcg
      301 cctcaggccc cctttatatc cactaaccta ctacttcagc agcagaagtg gagccacttt
      361 tgttaaagga aaaggaaaaa aaagaaaaac cctccacaga gctatagcgg ctttcttgac
      421 tgacccgggt ggttggctac atcatcttga catgtaatta tctaaagccc tttaatcact
      481 tccataaaga ttaattgcag gagacaacag cggaggtcgt gtcttcaaat caaaagactc
      541 cttggcagat tgacacagcg gtgtaacaga gttcagactt aggtgtttta taagggtcat
      601 ctactggaac tgaagcaggc tttaatgctg taattgcaag ttcagcaaag tgggttttaa
      661 agcataaagc tgcaaagaat tctattcaat gtttttagct tttttttcgt aggattttgg
      721 atattttcta catgctaatc cttagctgag cttatgtgtt gttgtttttt acccctaaag
      781 acaaaattct cttaccttca tctcttttcc tcttttcacc gttggatcgt ggaataccca
      841 gaatcccatt gatggagtag gatcctgcag ggtcattgga ggcgctggtt acaggcggag
      901 aggctgtgct tggtactgaa aaaaagacag caaccagcgt gaatttacat gaatatacat
      961 gcatgaacag tgtgtgcttt gactttatat gatgcctcat gctgaagctg gtgagctgca
     1021 gtgataccta tagtatggcc tggtgtcgac agggatgtcc cgtcaggaga tggatggaaa
     1081 ggctgctgga ctttcgttcg gatgatcctg gggaacaaag caaaagcaga tgtcagccgc
     1141 tgcttcctgg aaactgcccc taatatgatc aaactgtttg gtcacggcag ctgctgtgga
     1201 gatattctgt gattaagata tcaaacgcaa ctcatacgca agacgtggat ctggtcagtg
     1261 gttctacctt aaaggtgagg tgtggagaaa agtcttcatt gctcttttgg tctgagtgat
     1321 cttgcaaaca aagcatgaaa tcacatcaat tcacctcaat gatcagcttc actgactcta
     1381 cctggcccac gctaatcagc tctctccccc actgtggtgg gg
//
LOCUS       KCVB01000152            1424 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6964pri ultra-conserved element locus
            uce-993, sequence.
ACCESSION   KCVB01000152
VERSION     KCVB01000152.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6964pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1424
                     /note="ultra-conserved element locus uce-993"
BASE COUNT          418 a          287 c          312 g          407 t
ORIGIN      
        1 aattttaaga aataccctca aggtgtttct gagatgttgc attcatgagg acggatggac
       61 ggatggagcg gcattccgaa aacatcatgc cgccagtcgc acagaagcaa aatcacattt
      121 caggaaaagc aaaaacaaaa tttgagattt cttatcgaaa caacccaaca gtagtttgat
      181 cacattcttt gtagtgctta attaaaaaaa acataatttt aggaaaataa atcaaaacag
      241 agagaacggt ttttgcattt aaacctttca attgcaaata taattaattt ctgattttaa
      301 tcttattaga ttatggaatg gcagtgacgg ctggaccccc ctcctttcaa aagaaaaaac
      361 ccccaaaaaa ccctgcttgt gtactgattg tctaactgtc atgtcaaaac acactcctgg
      421 tggcagcagt gtctttggct ttgtgtttta cagggtgact cacagtcgtg atggacagtc
      481 aaatcgatat tttgactttg gccactggtt tttctcttag aggaggagca acgataatgg
      541 aggaggactg taaataagtg gaaggttctt gacaaatgaa tccattaaca tttgcctgtg
      601 tcgcctggcg agcagatttg ctgttttatg gagcaggaaa aaagagctag taaatctgag
      661 ggcgctgtaa gggtgaggag ctgagagcag gctggggaac acatctccca tgatgcagca
      721 ctttatctcc ctcccaaatg gcatctttat ctgcctaaat taagttttga ttccttgcca
      781 catacggagc tgcaaatcac ttttaatact gatatcaaga gcagcagtaa actgtgccca
      841 aagcgatgga aagcagcttt attgtgtggc acacactgcc gacaccacca ttcactgtgt
      901 ctctgtggct cacagttata aaaaaatgaa aaaaaaccca accccaaacc aatgctttgt
      961 gtgcaaatca tattatacca tttgtcatca ttcagcttcc tctctttcct gcagctctgc
     1021 acctggagta acttcagcta tctacctgta actctgtggg tgaggaagaa ggatacccta
     1081 aaggtgacat aatttatagc tgtgattaat ttccaattcc attttcattc agtgatgttt
     1141 atctgtaaaa ttagaaagaa gctgaggcct atttgtgtgt atgtgcatga tggtggcaag
     1201 ggggatggga agaggttgag gagttggtac cacatacaaa ctgcattaca ttacctctgc
     1261 aatatcatca gtctgcctgc atatctctct gtttgtttgt ctgcctatca atctgtctga
     1321 ctgtctgttg ctcggtctaa ttgatcacag cttggcggga gagacagcgg ccttgtgagc
     1381 gctcagcagg aatacgggat gtctgacgtg cagtgctgag gaac
//
LOCUS       KCVB01000153             618 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 6996pri ultra-conserved element locus
            uce-294, sequence.
ACCESSION   KCVB01000153
VERSION     KCVB01000153.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 618)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="6996pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>618
                     /note="ultra-conserved element locus uce-294"
BASE COUNT          197 a          126 c          115 g          180 t
ORIGIN      
        1 catcttatat ttcaatttgt tttctggtca gatttaaaag agaacatgtg taagcctgac
       61 ctcttacaca tgcactcaca aagtcatgca cacatagaca tgtgcacaca aaagggagga
      121 aaaaaatgaa ggatggagga aggtgacagg taaacaaatt cagtgtcata ttctcctgct
      181 gtcttcactg tgccacttgg cattttaatg gaagggaatc tgatgtaaca tatagtggta
      241 actacaacag cagcctcgta ccagagggga aaacttaact cttcagacct atgcattaaa
      301 catattgtag aatagagtgt aacatttatc actgtcattc ctagagagtt tgtgtaaatt
      361 taaatgcgct caagcgtgct gattccaacc cccccccaca atgtatgcgt acaatgccac
      421 cccaaagccc attttcctga aatccttctc attgttctgc acgaccatac caccaaacag
      481 ctattcatct ttggggaata tgctgtacag aaatgtcttt gtatgtgggg cctttttctg
      541 ttgctgtgtt tcatatagca gaatctgtgc acctattatt catggtttaa taataaaaaa
      601 aaaaagttaa atgaaaga
//
LOCUS       KCVB01000154            1518 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7034pri ultra-conserved element locus
            uce-645, sequence.
ACCESSION   KCVB01000154
VERSION     KCVB01000154.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7034pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1518
                     /note="ultra-conserved element locus uce-645"
BASE COUNT          440 a          319 c          364 g          395 t
ORIGIN      
        1 cacacacaca agtgtaacaa cacacacaca cacacacaca cacatacagg gtctctaatg
       61 atcctacggg gcatttttta aaagccttgc ggcactctga tcataagttc attgttagta
      121 agtgaaaaac aatgatctca gcgctaggag gcctctggga atctctcact ccaccaatat
      181 agaagcattt aagtcaaaca ccatggggaa ttaatgatag tggagttacc attaactcct
      241 acgctaacga gtttgtgagc tttggccctc ttgttagaca catcacgtgt gtgtttgtgt
      301 gtgtgtgttt gcttgtctgc cttagtgctt acgctggaga atcttgttga tctgaaacag
      361 acacgataaa tgtgcctgaa tgtgattttc acggttaaaa tgcaaacaaa acggcggcga
      421 tcaggcgagg cgctaggagt gccgtggcgt cttatcctct cagtctgcaa agagacgtgg
      481 ccaaggttat taaagaagga tgtagcagag caagatggcc gctgttgtga ccagaggcgg
      541 tgtggagaga taaatggttg acccagcctg gtgtcaggtt aggattgatg ccactatcag
      601 ccccactgct ttcccaatga ttttctcaaa tctctgatag agtgtgttat ctagttactg
      661 gcatgagggc aacggcctgc aggcacagag aaggtcaatc tatgaggaca atctttgttg
      721 gactaaatga gaaaaaagac ctcacaaaaa agaataaaaa gcagctgcta ggtctctggg
      781 ataagataag ccaaattact tctctttagt aggataggcc tcgggcaaaa catcagaaaa
      841 aaaggaggaa aaaaaaagaa gaagaaaaaa attaagtgag gcagccggcg tgctctctaa
      901 cttcaatgtt gtagatttca cctggaacct cggcttcctt gaactaatgt tgattcagtg
      961 gcagcagcac cttgaggaaa cggccgtgtg agtgtgtgtt tgtctgctgt ttaatatgca
     1021 ttcatctgct aaatgtcctt aacagcagat aaacaagaat atatatatat atacggagag
     1081 agaaccagtc agaaacgtca gaaagcacct cacagatcgc agagctcctc caaactctca
     1141 ctctatcagc cttttgctct ttggtctttt cataactggg ggaaagagtt tgactaatag
     1201 gttgtaattt accggccgag gtcctgatca ataattctgt gtcaataatg atggagaggt
     1261 ctgcgtgagg ggaaatgatt ccactcattg tctgagaaaa atgaaggtgt ggacatcacc
     1321 ccgggctgca ggcagatgtc cacaggtgct cggctggtat gatggtggag ttgatgagtg
     1381 ggaagacaaa taacacacac acagacactt acagccttca gtaaccttca tcactcaagc
     1441 ttctgtcgac cttcggtaat gaccccatgc cggtttcctg tgtttttctg ttttcgtgcg
     1501 atcgtccgtg tctccgca
//
LOCUS       KCVB01000155            1492 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7069pri ultra-conserved element locus
            uce-600, sequence.
ACCESSION   KCVB01000155
VERSION     KCVB01000155.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7069pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1492
                     /note="ultra-conserved element locus uce-600"
BASE COUNT          463 a          293 c          296 g          440 t
ORIGIN      
        1 attttcgtgc aaacgactca gtttcagaag atgagaaacg agatttttac tgagagaaaa
       61 gcatttaatg ctcctttgct attccttcat taatactttg aggcaacacg gctgacttgt
      121 ttggcatcaa agtgggctaa ctctcctaca ggctaataat gaaagacaat ctatttccta
      181 cgctgtttag tagtgcaggg aatatttctt tctccaaaag tatggtaagg gctccgagtt
      241 gttcctcaga agcctggtca aattaaaact caggcagaaa atgttgtctc atttaaattc
      301 tcaccttgag ctctctagtg actggtaatg gtccgagagg agctgacgct taaaaaaaat
      361 catctaggtc tattctcact gtacgctaca gtttggtggg ttctgtcatg cccgagcata
      421 tcagacgaca ttgtacagaa aaaaacagaa aatagtgaca atgtttttgc aggcagaatc
      481 atcagttaaa catagaaaat gcatgcaagg caggacgctg aagccttact tactctgtgt
      541 gctgtgctga gtctccgcag tttaattaca agccctaatt tccaatcgca gatagtgtca
      601 aaaaatctgc gactatttct cagagattcc ctttaacaag tggaaactta gtgtcctcct
      661 gcaccatttt atacatgatg agccgtcagt aatgggatta ttaaacaaaa acgtttcagg
      721 cgctggcgtt aacctttttt aatgcctaat taggttttgc aaagaaataa ttagattcta
      781 cagtgcttac actgcctata ttttcaaatg gcatctccag caaacacatc atcctgcgac
      841 actcgtgcca ataatctgcg gatatatgcg ctacagaagc agtcagatgt aagattttaa
      901 aacatgttgg gaccaaataa ggtgcactta aagctaaaat aattatcaaa cacaggctat
      961 atgccaacct gcaggaacat gttctggtcg agtacgccct cttgtggtag cctcctaggc
     1021 agcatttaaa ttattaatag gctatagcag gagataactt ggtttaaata gatcaagggc
     1081 gtaatcgttt gatgaataat gcacttttta tctcaaacca ggatatcaga aattgaagga
     1141 tgttaaccct ggctaaatgt agaaaagagg aagtctctgg tttttgctcc aaaaatgtgg
     1201 gcgttgacct tggcgcaggc gcattgcatt tttttttctt gacgctgaaa atatactcag
     1261 cgcaataact gcaatgttct ctgtatctcc atcatgccaa tgccttggta catcctcctc
     1321 agaattaatt gagtcttgtg actattgtgt gaagcctgca acaagtgaca atataaatac
     1381 actgcatata aacagcaaaa tgtggcatct ttctgtcaca tatgaaaatg aaaacatttt
     1441 aagaagaaaa aaacccagct accacagtgt caatgtgtgt ttatgggttt gt
//
LOCUS       KCVB01000156            1449 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7110pri ultra-conserved element locus
            uce-168, sequence.
ACCESSION   KCVB01000156
VERSION     KCVB01000156.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7110pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1449
                     /note="ultra-conserved element locus uce-168"
BASE COUNT          421 a          253 c          252 g          523 t
ORIGIN      
        1 tcatcactga ggcatgggct cttataatca agcagagtgc tgcacgttta acaggcacat
       61 taaccattgc ctcaaaccaa caaacatctg ttaacagtct tcgataccac acaaaatgat
      121 atacacttaa ggcaagtttt gctttaagat acagcagagt aaattgacag taaacacttt
      181 gtgctcgggt cgtgtaaaac agtgacttac agtgagattg ttgttgtgag agaaatgaga
      241 aacaacatgt caaaatgttt agtattcatt tcatagcttt cagttccaca gttccacagt
      301 ttttttctgt gcatatgtgc gtgtgtgtgt gtgtgtgtgt atttaagtgt tcgtgtggca
      361 cattttgtct tgtaaatgtc gtccattttc atatgttaaa attgtcctca tcacttggaa
      421 tcacaaaaaa atgtcatttt tttaaattaa gctgaaatta tcacacaagt aaaattgttg
      481 tgaattgtat atttttttat taactaattc gtaatgcttt tttttaactt agcaatatca
      541 tcactatttt attaatctaa atcttaacca ctgagttatg tatatagtgc actctgcatt
      601 gtgtatcaga ttagtgtgcc tttcctgaaa ccagagtgaa atgtgtaatt tggtttcacg
      661 tgtgcatgtg tgcatttgtg tctcatcagg tctatggaaa tgcaggatct agccagtcct
      721 cacagccgag taagtggaag cagcgattct ccgaatggtc ccaaccttga caactcccac
      781 atcaataaca attccatgac acccaatggc actgaaggta tgttagcggt gctgttgctt
      841 tttggttgct ttctttgtct ttcatactat tctacattta aaatagggct gagaacatta
      901 aatgtaagac tcagtgtgaa attaactttt ttatctgtaa gtagcaataa aagtaatgca
      961 agtgctgttg catttaaatt tttagttatg tttttagagt aaatatcaat actttttttt
     1021 gccttaatat agtgtttaac agtcagcaaa acaataaaga gagagtactt tgtaattgaa
     1081 ttttttttat ttcatttaat taattttttg ttttaaccat aaactatttt atgtcctaac
     1141 cctaaccctt gcttaccaac aattcaatac taccagtagt tgttttccag caactattag
     1201 ctgtttattt aagtagccat gtaattattc aaagcccaca gcatggaaat gatggagttt
     1261 tgcatgaggt ctctactgta catagttatt tactcaggtg attcaccttt gcttaaatct
     1321 gagcctttgt ttagtgaagc agtcatgcac acgagttatt aaaaacatct cccctgtcac
     1381 gtcatcttca ggttagtctg tgctttgagc tcagagttta tttttttgcg tattcatgac
     1441 acaggaccc
//
LOCUS       KCVB01000157            1634 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7194pri ultra-conserved element locus
            uce-758, sequence.
ACCESSION   KCVB01000157
VERSION     KCVB01000157.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7194pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1634
                     /note="ultra-conserved element locus uce-758"
BASE COUNT          451 a          359 c          369 g          455 t
ORIGIN      
        1 gagagacata aataaagata aaatgaggtg ccctaggtct tctatcccac tgaattctcc
       61 tctgccatct ctccaaactg atggaggccc cgtttaaatg aaggctaaat cttatttaga
      121 taagagaact gaactgctgc ccgggacgag agcatcattg tttgttactg atggctggag
      181 aggaagagag gagcaggagg aggaggaggg gtgggctgag ggaaagtggc tggtctttgc
      241 atgttcttat gctataagag agattgaatg ttgtttgtgt gtttgcagcg gcgcgagcgt
      301 gcagaccaca tgcagaggat atctgtcact gcacagatag acaggtatgg tttttgtttt
      361 gtgagtgtgc atttgtgaat tgagtccctc tgtaataaaa tttcattgta gtatatccat
      421 cctaatgtcg tttcctatct cagagttctt tggctgtgta ttgtatggaa tacaatatca
      481 atatccattg tttatagctt tcttttatag ctgaacatca aattgtgtgt ttgtgtatgc
      541 gtgtctgttg cacaaacatt ttgtttgagc cattgggcac acagcactca gctgcctcga
      601 gcagcacaag tttagaaact gttttccttt tttaattgtt gtggaaatta aataagagtg
      661 tttgtgacat gagtgttgta ggtaatgaga gcctgagagc agctgggagg ctacgggcct
      721 gtgtgcgcat cattaggagc tcagaggctc ccaggctcca cgctagcgta agccgctaat
      781 gaaaagaatg tagtcacaga tctcattttt caggcaattc ttgtgtaaac tggagatttt
      841 ccagtgtatg cacagtggaa caggggcccc acataaatca cgcaggctgg ctgcctccag
      901 tgaaagaaag agggggtttt gagcacatgc taaccacaga gacatatgct gcaggaaact
      961 ggaaatctgc agtggcacag ctagagagag agacactcac agcacctaca cctgtccacc
     1021 ccccctcccc caccacccac ttctccccat ctcttctagc tctctatcca tctccaccgc
     1081 gctgtccgac agcccaaccc tgatcctccc ccctctctaa catccctcct acccgcaggc
     1141 aaaccaacca gcaacgcctg ccatgcaagc cattgaaatg ggtcattttt taattaaata
     1201 caatattaat tactgcgagc atcattaccg atatgtgaga tttgactaat tagtgtaata
     1261 gttttggagt cacgctcatt aaatatgaaa attacagcag tagaaaagat tggttccgtg
     1321 atcaatcttg tatggacaga gaggaaatga tttagagaat agcttttatt gtacaattaa
     1381 ctccattaca acctgccaga caagaataaa gagagcaggg aatttggtcg gtgttaagag
     1441 tttcacacaa gtgtcgcctt cagctgctgg ctctcctcaa atgcaatctt ttgcccgtaa
     1501 ctagatgaga cacttgccaa agcatacaat accctcagag aagccttagt ctcatcaccc
     1561 actcaattag gacagctgct aaatggaaga cttgttgatc tcatctattt gacaccctca
     1621 gagttcattg tact
//
LOCUS       KCVB01000158            1656 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7233pri ultra-conserved element locus
            uce-34, sequence.
ACCESSION   KCVB01000158
VERSION     KCVB01000158.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7233pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1656
                     /note="ultra-conserved element locus uce-34"
BASE COUNT          446 a          379 c          349 g          482 t
ORIGIN      
        1 ataatataaa gaaaataaga attagttttt gaaaacagca tcaagtgcaa gcaatgacac
       61 attatttaca accttttcta atttaacaga actcctacag aacaagaggc agccagcagg
      121 aattccagta gcagatttgc cgtaatgctg ttgagttgat tagaagagtg agaggagagt
      181 gactgagaga gtgagtgagt gagtgagtaa gaggcaagga gggagtgagt gagcgagtca
      241 agccgcccct cttcccaaag cttatttatt cctccagcat ccagcccact gattaggcag
      301 ctacacacac tcttaatgcc tccccacaat gaatgagctg ctgcactttc aatgggctta
      361 tctgttgtgc tgcgttcagt aaataatgcc gcagctgtca gtggcctcaa acgcatcacc
      421 catgtagcta atgctatgtt aggattagcc gtgttgaatg aagtcttgtc atcgaacagg
      481 gggaagcttt gccaagctcc aaacagttta caggaccctc caccccaagt ggaacaaaaa
      541 gaatgtaatt aaggagagat gagagcatgg agggaggtgt atttcgacag ggatgcacac
      601 acacacacaa acacaaactc tcacatttac ccctgcaaat gcaaatgcct ctttgttagt
      661 catcatgcct tttaataccc agagagagta aaataccttt ttttttccac attatggaat
      721 ttgtcatgtt cagttgaagc tgtcatagct agaacagtgt gcgctaatgt gtgtaatctg
      781 ctttgcaaca catcaattat gatggatatt gctccgccgc catgcttcca gggtttttat
      841 acaagcctgc aggggaatca gtcttttctt gttaggcaac ctgtttagta aataatgatc
      901 cactttgagc cggggctgat gccgtataat gtgatgggtg tgcttgtgtg tgcatacagt
      961 atagcgtgtg tgtgtgtgtg gtggtgcttt ggtgtgcaag gatcaagcct agtagatcaa
     1021 aaaacactta cgtgcaaggc tgaaattcaa tcacgcctgt gctgtgtgtg aaaacccacg
     1081 tcacggagaa acctaaaccg cttctgagcg tgctgcgagc ccatatgtaa aaactataaa
     1141 tattatcagt gacttgtaaa agtgttttcc agagccatcc gatctaacaa agagacggaa
     1201 agataattat ttagacctta cactgtgtca aacagaattg attttttttc caaattacct
     1261 ttaccagtct ggttttttct tgcaaggatg ttccgagttg cacgtacacc acctctcctc
     1321 ttcctgtaat ctcccctctt tccagtaata cctctgttat ttgtctgttt tgcttccttt
     1381 atctcccttt ttcccgaact ttccactcct ccctgaactc tttctctctc tccacggctc
     1441 gtgggtgaat cctctatttt tgcaccgaat cttgccttct caagcaagag cggttcatga
     1501 actcccttcg ctcgcgttat gaattatgtc tcttcacctc gcgctctatc accaggacag
     1561 ggcggtagaa aacacagaga gagtggcaca gatttaatct tgtacctgct ctgaaaccat
     1621 tgtgtgcgct ccatttctct catttttccc ctcatc
//
LOCUS       KCVB01000159            1588 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7273pri ultra-conserved element locus
            uce-836, sequence.
ACCESSION   KCVB01000159
VERSION     KCVB01000159.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7273pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1588
                     /note="ultra-conserved element locus uce-836"
BASE COUNT          511 a          310 c          259 g          508 t
ORIGIN      
        1 caaaaaaatt gctttcacag actcttgatt aggttgatta ttgaaaaaac atcagatttt
       61 ataattgtca tcatagaaaa aaataattta aactcagatt acagattatc tatttctttt
      121 gtcaactctg ttttaaatga attacacaaa taattaccat caacgttgca tcctgcacgc
      181 tttgtattgc gcacctttat ttatttatgt gtttattttt tgggttgctt cttctcatca
      241 attcacattt aacacaggct tttgaacagc aacacgagta aaataaaaac atgcattcca
      301 gttcaacatg tatactgata ccagcacaag aaagtaatta gttttgaaca taatatgcat
      361 gttatgattg atttcatgtg gaggaactgc agagctgctc tcagatctgt gctgtgtgtt
      421 ttgagatgat gctgcaacga gaacagcaat gcttggcctc tgctctgtga cctgtaaata
      481 attgataaat caattttctt gactagaaca ccaaataatg aagcaatgca attcttatca
      541 attacatttt cgtcttaatt gttgacttca cttagcacaa agcagcacag cgaaacacca
      601 tgccattttt caattaaaag gtccagatgt ttttggaggc ttcagtagac acaggcagtt
      661 gtgagaaatg ctttatctgc attgtaataa tcactagaac atcagctcca ggctgcattt
      721 acatactcat gttttattcc agccctcttt ctaatgcacc gcttaattat actgagcgca
      781 gacaatgtca gacccccata gccccaaatt tattattgca ctgtgtgaag cagcaaccat
      841 caaacccaga gcccctcatc acatcataac acactcatca ccgctctcac tctatgaaat
      901 acttaccatc ctcttagtat tgaactcgta tcaaaattca ctttattccc atttttcttc
      961 acttagacgg aatgtttgca atatttattc agtaacttca ttggaaaaaa taacatgtaa
     1021 tgtcaaaaat aatatactgt ataagaaatt aaataacgtc aaaaatggct ttataaagtc
     1081 tctaattaag attattcatc ttaatagacc tgataaataa aaccgcatgt tttattacaa
     1141 ccaagtcatc agagttaaat gtttgcattg cacgtttctt caaaccacaa acatgttaaa
     1201 tatgacgtgt catatcaatg tatctacaat atttatttaa gtgacggagt gcagattaaa
     1261 tgggttggag gggatggtgg tggtgtgaca gctcagcgag acggctgcta acttcctgtt
     1321 ttggttttta ctagacttaa ataaattgtc ctgccatgtt tgtggcgaca aaactggtca
     1381 ttttaggcca aaacacaacc ttttcctgcc cctaaccaag tggtgtttgt gcctaaaccg
     1441 gacctcaacc acagctatgt aaatttgaaa atgtaaacgt aaagaaatgc aaagtttcaa
     1501 catatctgtg ggtttgatcc tggcaactgg gttgaccaaa atcatacatg tcccctaaag
     1561 agaagagaac tggttttggt gctgaatg
//
LOCUS       KCVB01000160            1474 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7318pri ultra-conserved element locus
            uce-71, sequence.
ACCESSION   KCVB01000160
VERSION     KCVB01000160.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7318pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1474
                     /note="ultra-conserved element locus uce-71"
BASE COUNT          446 a          298 c          341 g          389 t
ORIGIN      
        1 attttgaata ttctacttaa ttatgcaaag ggaatagtga ggtagatgag aagttttaag
       61 aaagactcat aaagtcctta ttaagagcag aggggattaa tgacggataa tatgagggca
      121 atcaaacctt taatgcaaag gccctcctgc aggcaaacag tatttaaatg agtcaattag
      181 acattcatta tctacaagcc tataagtgga gtcatatctg cataaatgtt ggtgtggaga
      241 actaatgaaa tatttaaaaa gttaacaact tagacatgga aaatgtacaa atattgattg
      301 ctagagggct ggctacacgt tgtcatcaga tcgggggaga atgcacgata cccactggag
      361 attgtctcat aaatcataat tattgagtta gcatgatctc tcctcccaat tagctatgta
      421 gcattgttga ggtaaaataa gagaggggaa aggggaaagc cctccaattg tccattataa
      481 aatcacatca aatcctgtac acggatgtct ccagtcttac tgtaagtgcc tctgaattat
      541 tctcattcta acaaacccac attaaaataa ataaatatcc acaaaaacat acatattgtg
      601 cagaggatga cttgaataat gtaccaaact atgcagatat atcattgtca ggacctcctc
      661 cctcctcctc ttcctatcct tctttctttc cctttctcca tgtctgagcc tccactcttg
      721 tcagttgccc cagcacacaa ttactgtgtg tgattgctat caggcgggac ggtcggggag
      781 gctcccaaat tagggcctaa ataggatttc ctacatggcg aagggcacag gcctggacat
      841 ggtaatgaaa gcctatacat cacaatcagg ccgacaatcc tattcatact gcaacacatt
      901 actctcactc ctcataatta tagaggggtg acagacgggc ctgtgaggca tggtggagga
      961 ggcacgagag cgaggagaag gaggaggcga aggtggtgtt ggtggggggg tgttactggg
     1021 aaagtggctg agcagtgtca cagttagttt cgagtggctt cttctctata cttatgtgat
     1081 ctcttgatac ctggagtgac cgaattagga gggagtccac tgggttcaat tgtacatagt
     1141 tccagtgtgc aatccagcca cctatagatc tgtgtaatta cagaaaagag ttctaatttg
     1201 ggcattttct cccaatgggg agattcttcc aatgggacac tgaccacatc ttccatcaga
     1261 ctgactgaca caggcacaaa cacatacaca tacacacact ccctcacacg gagcgatgtg
     1321 atgagatgtg agagggacag ctttgcgatc aataccatga gcaggaatca gcgatgggga
     1381 gaataagaca atggcctatc agattgaggt tgacttctcc ttgtatgtgt ggcaggcctg
     1441 tgacaataga ttggccgaga aatccgatgg cact
//
LOCUS       KCVB01000161            1592 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7366pri ultra-conserved element locus
            uce-1303, sequence.
ACCESSION   KCVB01000161
VERSION     KCVB01000161.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7366pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1592
                     /note="ultra-conserved element locus uce-1303"
BASE COUNT          488 a          329 c          304 g          471 t
ORIGIN      
        1 agtatgacac tctctagtga acatgctttc aaatgatgtg gaccattaat atgagattgt
       61 tgctgtcact ggttaatccc tccacatctg caactgcagt ttccatacac gtcactgcta
      121 aatggcagca ttacatagat ttacattaca taaaatcaga caaactaaaa ttgagctttt
      181 ttccttttta gtttctattt cacgcccaca gtttctttgt ctctctcttc tcattttttg
      241 aataaccagg tagtcagtta cataaatact gtaataacat taataacatt tactaaaatt
      301 cctgtcccca atagaaacat tgtaaaagct gttgagtaga gcagcgtctg accataggcg
      361 catatttgat ccatctgaag tcatctccat agtgacccac gatgaataaa taaccagttt
      421 gtcacaaccc caaaatcttg gatccaaatt taaaatgact tcttccttgc ctcatcatac
      481 ctgcatcaat gctatttaca ctttcgacag ttactccttt tgaagaagac ataaaataat
      541 tggacttttt tcactaccat gtgtgtgaaa acacagtagt cttacactga caaaggcagg
      601 ggaacaaatt agggattatt ctggaggtgt gtgatgcctc tcctgcaatg aagttcctca
      661 cactccattc tgagtgaatg taggtgactc ggggagcctg agcggatccc agaattcctc
      721 attatccgcc cagcagggag gatcaggtcc gctcgctctg tctcagagca tgctcagccc
      781 gatgagacga gccccagagg agcacagtgc tgggccatcg ggggcaagcc aggtctgctc
      841 attatatcta actcatcctt ggcatctctg caaaccaagt gccacttagt tcccacagga
      901 ggggtctggg ctggatcact gacaagatgg cagagcaaac agcagagtgg taataggaaa
      961 agctttacat caaaaaaata tgtggacaga ggtataatgt ccagcagtac acctataaga
     1021 ttaatatcac aacataaagg gatatttcac ttatttggtg acggcattgc ttatttgtag
     1081 gattcatatg tgcaagtaat ggtatgatta ctatagtgtt taacagagcc agatttacac
     1141 tagacatata gttaaattcc cattaaaggt cactgccata agcatttgaa aaaagacttt
     1201 cagatacaaa tggtggcctc acccttcagc agcaaaaaca aaaaacacta catgggtgtg
     1261 gtatagccca ctagtcgggt atgtatttac atattcattt atgagtaaat ggaataaaaa
     1321 tcagttcttt cttttttttt aacagtaact gagaaaaatt caccattgtc atttagtttg
     1381 tggggcaagt gcaaatacat aactcaatga ataaacatac aatttgagtg aagcgtgttg
     1441 tttcatttat caactctatt tctggaataa atgaatgcag cctagcacag caggtgaatt
     1501 atgcaagtgt agagtctcag gcctttggct aggggcaagt gccctctatt tccctttact
     1561 ttttaaaata ttctcctaag taaactgtgt gg
//
LOCUS       KCVB01000162             756 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7400pri ultra-conserved element locus
            uce-459, sequence.
ACCESSION   KCVB01000162
VERSION     KCVB01000162.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 756)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7400pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>756
                     /note="ultra-conserved element locus uce-459"
BASE COUNT          238 a          142 c          137 g          239 t
ORIGIN      
        1 ttgaatactt tacagacatg ttaattgctg atacatcacc cacaggtcaa cttaaatgca
       61 aagtctgcgt cttgaaacca cattcatttg ttacttcctc tatggagagg gtagataatc
      121 taatcaattt tgagagaaaa aaaatatgca aaaacaaaag caagaggtgg gggatcgaac
      181 ataacattaa ggaacattga gaaatatcca attgtttgag caaatgtaaa atgccaaagc
      241 ctcttagaac attgtaagtc tttattcagt aatacattat catgtatatt tcatagaggg
      301 agattactaa aacggaattg tgtgtctttc tgttgtatgt ctttataatt acaccttgaa
      361 gccatagctg attttttttc cctgcctttt ttccctccat attcatgaaa agcagacttg
      421 taatttgata aagagaagct tgtgttctca acttcattca aacggcatca tggcgatgta
      481 ttaaagtggg catctgtgtt ctcgcggcat taatctcttc aagcacattc cagctgctgt
      541 gatgtgcgag agggagataa gtcttaaaca gtctaaagct aagcaaacca gacaactcta
      601 ttattggctt cataattgct taattaaact tttgtcacta atcattatga gggcaataga
      661 gctgttatac aggctttctg tcgagcagga aaaaaaaatc aataaactgt gttggggagc
      721 tgatcccccc acgtctctct ctctctctct ctctct
//
LOCUS       KCVB01000163            1369 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7478pri ultra-conserved element locus
            uce-374, sequence.
ACCESSION   KCVB01000163
VERSION     KCVB01000163.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7478pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1369
                     /note="ultra-conserved element locus uce-374"
BASE COUNT          448 a          307 c          290 g          324 t
ORIGIN      
        1 ctaaaacttc taaaaaatca tcaacatata cagaagccag tcaaactggg ctatctgtaa
       61 atccaaatct gtaggccaga agcacctaat tttatcaagg atatctacac acttcacaca
      121 ctctttggaa actttctggc tcacggtctg tgaatcctcc aatgtaataa aacgacaaca
      181 actagaagtt tgagggggtg gaaatggaca cagagataat tatcgaacat gtaaaaacgg
      241 attatgagtt acaccacaga tggagggaaa caagttacac ggggaaggaa gcaattaaat
      301 agccatgagc cggacccaac cctgcgcaac atctgccgag accagtattc agcactgcac
      361 aactgacaaa ttagtgtaat ggtctaatag ggacggatag agggagcgaa tacaataata
      421 agactgttta acacaggctg gctgttctcg ggctaccgga tagtctgcag aaacttaccc
      481 agagacgcgg tcctcgtcct ggagagcgtc cggtccatac tgaggaggac tgagcactag
      541 agacggtctg taccggtcaa gtaacactta cagcctgcaa agagagagtc agacgaagcc
      601 tcgccgtcag tgcgattata tgttatgtgc aatatactgt caactcccca aaaccataaa
      661 acttacttta atggctacca cgtgaccgtt tatggccagt gagcctatct ggccgcgctc
      721 cggatgattt ttacgatcta attcatagac aaaacccccc attcatttag cacccaaatg
      781 tcagagagcc agaatcggcc ctaataaact ttacagcttg aaaagtcgag ctgattctcc
      841 gggaatagcc caggagtctg tgcgcaaggg gacgcacggc ccctgaagcc ggttagtgcc
      901 caaggacccc caaaagcccc ccagctccac agcaaggtga gtaagacata aagctttcat
      961 tcagggggac ctgttgaatt caatgagatt taacctgagg aaacttgaac aggaggcttg
     1021 cagattttag gacagcaaac tttgactctt gtctttcatg cagcagtcgt ggcaaaagcg
     1081 caaatgaatc tattgaatat ttgacaaaaa cgtacactca ctgtaatgtt gccgttccag
     1141 tagaaagttt attataatgg ccatcatcat catcgtcatt attattatta ttatagtata
     1201 ttcttgactt ccagtctttg aaaaacattt acaaaaagct aatcacgatg gagttttaca
     1261 cataggcaat ggaatttact gttgggacct ccaaataaaa acagctagag aaaacaaaga
     1321 caaaaaaaaa cacgttttta ctaagatgtt tcgctcaggt gaacaacgc
//
LOCUS       KCVB01000164            1056 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7524pri ultra-conserved element locus
            uce-798, sequence.
ACCESSION   KCVB01000164
VERSION     KCVB01000164.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7524pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1056
                     /note="ultra-conserved element locus uce-798"
BASE COUNT          328 a          179 c          209 g          340 t
ORIGIN      
        1 tgaagacgct ttagtttccc cttttttgac atgcaaggtg acattgttta catattgtta
       61 gtgatttaag tctttctgtc tctttataat attcaacatt tgggagaacc catcataccg
      121 ttcttaaatt gttaaattat aataatgtcc tattttttgc aaatataatt aaacaagtta
      181 aaatcaaaac caagcatatt taaactgata tctttgcaaa aacagttttt aatttcattc
      241 tgacttaaat aaaatactgt gatcacctca ctctcctctt gcatctcttc cttttttttt
      301 attccgctgt cgtctttttt ctttccacct cgcctctcat ggcagtcagc agtggaagga
      361 tacttagcac acaacagtta atacctgtct aatttattag tttcctgccc cagccctgaa
      421 gggccattag caacacacac tgacacagca cactggcctg tgtcgtaatt tattgtctta
      481 ttacagtgct gcagcggcat caattaatta tccattaaag cgctgctgtc ccaggattta
      541 tggaggggac tgtgggggct gtgggagata cagtaggaga ggaatgaggg agaaagggag
      601 taaatgtgag gaataggagg agggtgagca gatatatggg ggaggatgaa agatggattg
      661 ttgtttttgg gctgcgcttt ggttcgaaag ttgggaaaaa ataaagtttg ctgacagtgc
      721 tttaaataaa agcaacacag agtctgcaaa tcaaatattg ttcttcctgt aaacaaatat
      781 tgtttaattg tgagattata aatcaagatg aaagctgaaa tataatcttt gctaacaatt
      841 taatttccaa gaggtggtgc atcatttatt gtgcatatcc aggctagtca gcaaactcaa
      901 tgcttgaggt caagaggagc aaagaagtgc agagtttgga cataaacatc attacagtta
      961 ttgcttgaat taaattaata tattcacaga tatcaattaa cacagtctag gggctgttta
     1021 atagagtcaa agttaacttc actacctact agaaag
//
LOCUS       KCVB01000165            1504 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7567pri ultra-conserved element locus
            uce-349, sequence.
ACCESSION   KCVB01000165
VERSION     KCVB01000165.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7567pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1504
                     /note="ultra-conserved element locus uce-349"
BASE COUNT          426 a          313 c          310 g          455 t
ORIGIN      
        1 atatctatga aagatttcgg actgatgtgt cagacagctg gaggaaggtc tggctgaact
       61 cctcatcgtt cttaaggatg cagtgatggt gcatttgcaa aataaagtat gaggggatac
      121 aaacaggact cttacagcac gatccaaacc tgcatcataa cttacctttt ttaacatgta
      181 ggctgttatt gttgttacag acaacaggaa gtgggaggac agtggtcagc caatggcagg
      241 ctacttcctg tgactgcacc tcatatgaag ttggggcagt agaggaaaga aaactgttgt
      301 tctccctttt agtttaagcg tagtcaaaaa agattttgga tggagatata tggcaggtct
      361 tcgcctgtga aacacatctg taacatccct tcaacaattg ctaatatttt ctaatagttg
      421 aatttatgtg cacattgtta taaataaatt accttgtatt tatcctgctg ttccaggatt
      481 atgtgtatca cttttatata agctgtatat ttgttgtgct cggggattaa agtaatttta
      541 attctcctca aaacaaaaca gaaattcata tttcggtgca tttgctcacc atcaattaaa
      601 tgaaaaaaga catcctgata cactccttca ccatcctcga cacctttgct taatccactt
      661 acaatatttc agctgcttcg caaacaaatt gatatcatca attgtgatgt tggcaaatac
      721 cccagccaat ctgctcatga atgtttatct cctctaacac tcacccacct gctaagaggt
      781 gccacgcttt aatgaaattg tttggactac cggcgcccgt tgctaaggaa ctgtgtgtct
      841 cctggttttt tgcctgcaga tttctgtcat caaataatga ttagagatga gcacacctca
      901 aggtgctcaa gccagtgaag ggtgtattca gagctagatt ggcaaacagc tccggggcga
      961 gaagagacac tgaaaaacga gcctttgtat gagaagcctt tgctggcatc gactccagtt
     1021 gtggcctttt tcaaaagaat caccgatggc ttcacaactt aatgcgattg gagacgcgtc
     1081 gatgtttgtg ttttgaaggt aggatgaccg ggaattcggc gagctgtgga attacacggc
     1141 tgagtattct gctcctctgt acctctggca aggtgtcagt ataaacctgt ttccacaaca
     1201 acaacaacac ctcttcccag cgattaagga ctgtcatagt caactttcga cgctgctcct
     1261 ttcatccctt ttttgaagtg tgatttcttc tcctttcata tttttcaaaa cacacatcag
     1321 cacagagatg ctctcagaca taggtgacat gaaaagagtc taagatcagg tgtttcatga
     1381 atatgtttac acgtagcagc ttttcactga gaaattattc cactcctgca gaaaaaaaag
     1441 gtttttgctg aagacatctg gagggtcgat gtttgacttg atttgtgcta cttcacgcta
     1501 aaca
//
LOCUS       KCVB01000166            1783 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7612pri ultra-conserved element locus
            uce-1087, sequence.
ACCESSION   KCVB01000166
VERSION     KCVB01000166.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7612pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1783
                     /note="ultra-conserved element locus uce-1087"
BASE COUNT          504 a          422 c          388 g          469 t
ORIGIN      
        1 ctaaagttca aattcctggt gtgaagcgtc ataatctaaa gatttctgta tttctaaaac
       61 aaatttcaga ttctatactt cacagtaatt catcaattcg agttttttag ggtaaacaat
      121 tttataaatg aggtctttat taactagagg aagcaagttg ccaaagtaac aataacatat
      181 gtaacacatt tgcatgcata acaatgatat tcaaaattct cagccatgta ttttgacaaa
      241 tgcagctgga atgaatgaat gtttggataa atggctctgc gggggacgac ggagccgcaa
      301 aaagcaccag aaaaagcaca aacatggtcc ctgttagttc tattgaaata agaggtctag
      361 aggtcctatg tgtctttgaa gcgaagctct gtgattgtgc agacgagcag acagaagcta
      421 atgatcgcta cacagcacac agggaccttg gccagcagcc cgtagttcac tagagctgat
      481 gcaccaacca aacacaacac actgtgcaaa tggactcggg gagcccaaaa cagcggcaca
      541 tttaagaggc atagtgggaa aaattccacc aatctgtttt gcattctcag caccaacact
      601 tagaccaaag gttttcacag cttttgttgg ttttgtattt ttttctctct ctctctctct
      661 ctcctttgga agcagaaatc ctttgattct caactctctc tgtaaaaaca ttaaaaaaga
      721 taatccattg gatctaattg acacaaagcc ctccgccccc cctcctcccc tgcctctatc
      781 ctccgcctat ggccctcatt tcttctgaga aaggccaagt gtttttgtcc tgtaaatacc
      841 acggagcaga gacaaaaaag acgtctttaa aatgcagtct acaatccatt acacaaattc
      901 cccctgcata tccattcagt aattattcat tagaaaacat ttaacgtcac agtgaatttc
      961 acactaggca gtcgaaacac aatggaggag atttggccta ttttggggtg aggagttggg
     1021 gaggggttgg ctgtacagag gaagggtgaa ggcgaaggag gggggagggg gggagcagta
     1081 tttgtctgat ctgagaacct ctgaaacggg gccgtcttga gacaacggga cagactaaat
     1141 agtgcagtaa cggtctgtgc cccgctgctt gctgtgacac tcatttagtg tgccccgagg
     1201 gcgaacaata gaggcttgtt cttagcaatc ctgtgctgta atttggactt cattactttg
     1261 ctcacacggg gccccttgga aggatagagg aggctagtca gatgagagtg tttttttcca
     1321 cctgtcagcc ccgcaccccc aatgctctca cccctctcct caacaatacc cctcaccctg
     1381 tgtccccacc aataaccctc acccgacccc ctaaccatgg ccgcctcccc tcccctccct
     1441 cccagccacc cactttaagc caatgagaca ggtgaacaat atcattgaag gaaccctttt
     1501 ttggacgtca gaatcaatct ggcgatgtga tggtggtggt gctggtgtgg ggtatctgtg
     1561 acaccccctt tcttcatggg aacaaacttc tctcagggca gcacaagcag acaatatgct
     1621 gggacagcgt gacttaggga tctgccatac agtattacaa gtaataacat gaacagataa
     1681 gctcctgtca ggaggaggat taatgaattc agggctgatg ataatgctaa tgtcttaggc
     1741 tttcattcag tgcgctgaca acatctaaag gtttgttgtt tcc
//
LOCUS       KCVB01000167             878 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7649pri ultra-conserved element locus
            uce-720, sequence.
ACCESSION   KCVB01000167
VERSION     KCVB01000167.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7649pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>878
                     /note="ultra-conserved element locus uce-720"
BASE COUNT          288 a          142 c          179 g          269 t
ORIGIN      
        1 tattcactga aaagaataca gatcgattta tattgatgtg ccttatgaaa tgttgaggtg
       61 agtctgagtg ttgatcgtaa tcggatttgc ttattcagat tgtgatagca aataggccga
      121 tgaatatttg cctataagaa gcagcaaatg cctgaggtct ttttttacat caactagtgc
      181 aaactacagt aagcagtgga attcaggacg tgattttaac agattttgca atctatttga
      241 aatggtgagg gcctttgtca tagaagaatg ctattacttc caaagggaat gaagtttttt
      301 ttttcatttc ccctttgtta gcactgagtg aaaagaaagt aaagccaaag tcaattacat
      361 ttgataagca atgtttagtt tgactcccat acttgtctct ctgtagggct cttttaggga
      421 cagcatgagg gaaaggaaag aagaaaagaa aacacaataa gcataacagt gacaggtaga
      481 agagaaatgt gcacatacaa tatgctgatg gtgcatcaag aaaaggtttc tgggatgcaa
      541 ttgttttctg tcaccatgca aagttggcgt acccagagaa agaaccacac aaatctatta
      601 acagatataa aggaaaagac tttttgctcc aactttttgc aagatcattg gggtaaaata
      661 tggcaatggt acttgtgtgt caaaagccgc agtaaatctc ctgaagcatt agcattttca
      721 tcctttcaga tatttctttt acatctatgg caatgttagt gaattatagc ctcttgtcat
      781 gagctccaat gcagtacttg tattgcatac ataaattatc cactgaacaa agctcagttt
      841 tacacgagtt ttacagactt aggaagttac agaaacct
//
LOCUS       KCVB01000168            1809 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7691pri ultra-conserved element locus
            uce-499, sequence.
ACCESSION   KCVB01000168
VERSION     KCVB01000168.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7691pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1809
                     /note="ultra-conserved element locus uce-499"
BASE COUNT          518 a          420 c          333 g          538 t
ORIGIN      
        1 gacagtcaag acagcaaggg gaaggtaaaa ggtgaagtga gccaattttc catatcagag
       61 gtggtttgtt tgccccaagc ctgagttgtg cctaaagtgc cataacatta cagtactcct
      121 acagatgaga tcaaaggctc tagcaaggga tcctccgcgg ttttactggc aagcggccgt
      181 cctttcgctg aaatatcttt tcagggcttt ccacacattg actgatcaca caaaatgcac
      241 tcttaataga gacaggaaat gccacataca aaaacttcct ttctttctaa gtatgggcta
      301 atattcagag attaagcttt ctcgttaatt attttcattt taattagtaa atcttaatta
      361 acaatgaaca tttaccctta aaattttttg gcttcggaat gaaggagggg ggtgaagaaa
      421 ggagagaata aatatgacaa tctttttggg acctccgggc aggattactg atgcaggctg
      481 ccttagttat taaacacgtt ctaaattaaa tgcctccatg aattttgcac agaaatatat
      541 tttcatagtt ttgcatgcca aatgtgacat tttatatttc agattcatct cgctgcagta
      601 attttaggga aatgtcatat aaacaaagat tttgtcattt accaaagaaa attactatgc
      661 gtggcaggtt tggccattgc tttctctccc tctctttctt tctttccctc tccctctttt
      721 gccccctatt ctgcctgtgc cggttgtttc aagtcagcaa gccataaaaa atctttatct
      781 cagtgaaaca cagcaatttg ggagataatc gaatggtgac tggggggcac aaacgccagt
      841 ctgaggaatt ataccacaat ctcccctgca tttacaacct tcctcaaagc cagttgtcac
      901 cctggaagga aagcccccga ctgaagattc attacatctt taagccagca cggtggcatg
      961 gcagcctcat ccccagatgc aacacaacag cacactcctc ccacagctgg tgatgctacc
     1021 gctaaagctg cagcttctct ctttcccact tggttctctt ttctctcctt ttcctctgtc
     1081 cccccttttc atctccaaag actccttctc cctcctactt agattcctaa aaccacaaac
     1141 atcatccaca caaagcagga aaaaaatctt agattgtata ctgtaaaaaa ataatatgac
     1201 tggatagtta gcattctgta tatggaatcc ttttgctcag cacagtgtag tgcctgtgtg
     1261 tgaatgtctt tgcctttgtg taggcatgtg cacacacaca gtactgattg gtgtaatgaa
     1321 aggagtgaag gaatttggaa aactagaaaa taagggatat gattttccca tcacaaacag
     1381 gtcccattca tcacaggatt tcatttcatt acatcatttg actgtttttg aacaaacaat
     1441 cctgcctttt tttgatacat tgtaaacact tcaggataaa tatctccttc tgtcaaacaa
     1501 ccatcatgta gattagcgag tatgtcattt cctgtttgtg tgctttctgt ttcctataaa
     1561 tgtatgttac tcagtcaaca acagtcacag cgacaaacag atctcacctg ccacatgggc
     1621 agtgatgctg aagaggagga ttctgggagc tctgtcgtct gaccgtattt ggccatggct
     1681 tcctcttggc cttcccccag tgctctgagt gacgggcttt caatggttgt gctgctacat
     1741 gcacaaacac acacacaaat acgtatacaa cacacacaaa cacaaaaagc taaccctgtt
     1801 aacagccac
//
LOCUS       KCVB01000169            1991 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7733pri ultra-conserved element locus
            uce-678, sequence.
ACCESSION   KCVB01000169
VERSION     KCVB01000169.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7733pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1991
                     /note="ultra-conserved element locus uce-678"
BASE COUNT          614 a          378 c          371 g          628 t
ORIGIN      
        1 aacactgaat aaaacgactg ctttcttaga gtttaaacat tttgcaaaca ttttggataa
       61 tgtaagaaca caactcaact atatacaaaa aaaggttaga gtcatttgtg gacgttaatg
      121 ttgaactgtt acatattgta agtttgtgtg tctgggaaaa ctgtcgttag aaaccccctc
      181 ccctaatcat cactgaaaaa ctttgatcag acaacttgaa actcctccag tttgtctcag
      241 gttattttta taagtcataa aacattctga gagcctaaag ataccgactt cctttctgat
      301 tagtgacttg ctgccctacc aattcaaatt atcttagcat atttgcacta tagtgcacct
      361 caggattatt acaaatgctt tttgcattac gttcctgccg cacttgaaaa aaaattatac
      421 atgtagtgtt gtggttcatt tgtgatatgg gatcaatatt ctgtgcatca gacattttac
      481 tcctccagtt gacctaagtg acagaccatt taaaacagag agagaccggc tcagctgctc
      541 tgcacggcaa caaagagaga ccacattatt aatttatctc aaaagcacag gtcgatatga
      601 gaggcctatt gcttacacgt tttgtttttg ttggcagttt ttgtgtcata tatagtgtct
      661 ttaaatatct ggtctctttc tgtgatttga tacctgaact cggggtgtca aacgggatag
      721 aaattagttt tgtcatgtga aaattgttgt tctgtaatat attttactaa cagaaaaata
      781 gatactttca ccagcatcaa agaaagaaag ctgcttctgt ctctttgttt ctctgtggag
      841 atgtggagaa gatatttacc aagacgatgg ggaggggtgg gggggagcag agaggaggag
      901 aggcattaga ggcgtccaca catgagcaat tagtattcca gatcctgatg gttctaaaca
      961 caaaaaggcc tcaccaaatc cccacggcgg aggtgtcaga cggaaaatga cgaaaatctg
     1021 ctccttgaca gggagtcatt tatattttag cttcgttcca ttatcttaac gaatccatca
     1081 tcagccaatg caatattaac agcagaaaat caactcatta gatccagggg gatttaatca
     1141 agattgcaat catgtatgcg attcctggag agttggcttc ttcttttttt tttctttttg
     1201 gtcctttcca cgtaattgcc gcatctcaga tcttaataaa ttattctcca tctgagattg
     1261 atgccttttt ttccttgcct ctcacgccat gcactcactg tctccaacta taatatttac
     1321 cataaacatt attatgtcat gggcacacag tttaaaatgg cctttagcta actcgcagaa
     1381 gtaattggct tctccatttc tctctcgttt ccttgctaat tgcccagtga tattgctcac
     1441 agttccctca gaaagaaaag aaaaggcctc ttttgcacaa gctgggatat gttgctagga
     1501 tttttcaacc tttatatgtg aatgtagaaa gaatttcctg tgtttgatac tagttaacaa
     1561 gacaagcaag cagatgaaag accattccac ttttgatatg taaaacttca tgtgaaaccc
     1621 cggcatcaga gagaaaataa aaagaactca aatttcaggt ggggatattt tagtcgtttc
     1681 tcagaaaaat gcttagcaga aaaggagctg cagcagatct aagagaagtc tggatgtttt
     1741 accagacaaa agaacaaagg ctctgctctg ccctgtaact gtgtccattt acttgtgtcg
     1801 acattactgt agacacttaa catttattat taacacttat ttgctgcaac taaataaata
     1861 aatttcaaca aatgtatttg tataaaacca agactttaag acacacatga cagacgagtt
     1921 acaatgtatc agtttgaagg tgataagatg tagctacaca taagatttct ttgtatacat
     1981 cttagtgtaa c
//
LOCUS       KCVB01000170            1586 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7780pri ultra-conserved element locus
            uce-1122, sequence.
ACCESSION   KCVB01000170
VERSION     KCVB01000170.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7780pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1586
                     /note="ultra-conserved element locus uce-1122"
BASE COUNT          457 a          370 c          318 g          441 t
ORIGIN      
        1 ccctgctttc tgagcctttt gggtcactga ggtgtcaggg gctgaggggg tcatagaccc
       61 tgaaatccag ccacacacac ctcaacacta tccctaaccc ggtgtaagca caactagacc
      121 ctaggctctg aggtgggttc cctcccacca ggtgttcggg atggcataca tgtcttctgg
      181 gtatggagtg ccgctggccg cccaggacta cacatggtgc tgagggtcaa acatggagcc
      241 atgctggacc agctgtcgct ttgctgttct ccccagctct aagccaggcc cgctggagag
      301 gaccagatgc gcacactaag tcactcttct ccagcttaca gcaacatggg tctcactgtc
      361 tgacctcatc gctttggcat tattttgcca agatcattgc ggtaaagcaa gcaacactgt
      421 gatggaaaaa aacccaactt tggttctctg cacaacagca cagaaagtga gcaaacagtt
      481 ccttagtatt ccccaataat tggcagggtc actgttctgt cttcccattc agtatacaag
      541 ggcatccatt aatttgaaaa ggctaagcta ccatgctcac acatgtacgc acccagatca
      601 cagaccgcgc tgctttggaa gcagcagcac tccatcttct gtctaactgc agctaaaagc
      661 atgaaagcag cagtgagatt tcatttggct aactgactga aggcactgag ggagtaaagc
      721 tcgctggaga gatttataca cccagcctga tgtctcctgc tccctataat agaacaaaag
      781 cttgttgtct cttccaatta cagatgtcaa aacgcttatt ttgagggttg tgatcttttc
      841 ccttttaatc tgcaaagata aaaggtctca gcttcattag catgtgttct ctccccccag
      901 actcagattc cctgcaagca gccagctcag ccttacagcc tattacccac tcttggggcc
      961 attaaggtct aacattaatg atgtgtaatt agcatatgat gacaaataat aacaatgcac
     1021 tcccattcga tgtaatgggg ggtaggataa taacatggaa tcccactgtt tcaaccctac
     1081 ataaagctca caatatctat gttttattgc ccactctgtt gttcagaggt acaatggagt
     1141 gggatgtagt actctcgcat aatttattca aattaatatg tttatcaaga aatatcacaa
     1201 ttacaataca tcagtaagtc aaagtgctta atctgcacaa agcccgaatg tagtttaatc
     1261 atgctcaata acgactgatg taaattctag tatattttta agggctagag cttttttatg
     1321 tttgtgttaa aaacatatta gaatattgta aactaccact attatattat aaccttagag
     1381 tccaacagtc tgctcagaaa tctctatatc tcctctatta gccaagacgg ccaaacgggc
     1441 gagtgatggg gctttttgag ctcttgccac atattaccta tatccctaat ttataaattc
     1501 tataaataaa tctattaaag ttaatgacag caagaactca agttaaatgg aggggtatta
     1561 gtagacaagt gatatgctta gaacag
//
LOCUS       KCVB01000171            1274 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7822pri ultra-conserved element locus
            uce-1167, sequence.
ACCESSION   KCVB01000171
VERSION     KCVB01000171.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7822pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1274
                     /note="ultra-conserved element locus uce-1167"
BASE COUNT          380 a          224 c          279 g          391 t
ORIGIN      
        1 gtggtcgacc tctgcaaaaa acatctttcc ccaacttgca ttgatgagac aaatgtaggt
       61 ataaaacctt aatcccttat ttattctcct catgaagcct caatctctga tcacaaagca
      121 ggtaaaaaga agctgatgat gttcaaatgt agattgtaga tttgtttgag gaagtgatga
      181 ccaatatttc ttcgtttgag tgtatatgag gtcagggtga gagagtgcgg atggataatt
      241 taatgttgac cggattataa ggagtttaac tgtgtcatac taaatgtaac agtcctgtgt
      301 ggtgtgattg attagtgctc tgactgattt cactccattg actatcataa attataaatg
      361 gcttggatat cacattactg accaattaca gatcacagac tgagaatgtg atcgattccc
      421 gtcccattat acactgacac acttacagat gcattttagg tagagacgtt tggaagaaga
      481 gagaggacta aaagaaatat atgttgtgtt tgatgctaag aaggcaggca tctcctggct
      541 cacacagtgg agctgttatt gtagaaaggc agttgtttag gtctgcagga acataaggat
      601 atgtgtgact tttttctctt aagtccaggc ttgtgtctaa tatctgtatg cgcatgcagg
      661 attatccatg aagatggctt ttctggggat gacgtgaagc agtataagcc tgtggtctac
      721 agcaacacca tccagagctt ggcagccatc ctccgagcca tggactccct gggtatcgag
      781 tttggagaca aggatagaaa agtaagtctt actttcaccc acaggcagaa atgttcacca
      841 acagactgcc taggaactga gattttatta atacatcttt gaaatttagg gtttgttcct
      901 tagaacagaa gaatttaaga aagatcagca gattgttttt ccaatggtga taaaaatcac
      961 aatatctgtt tgggtaaaga cgccccgatg tccctcatat ggactgcatg cttgacaatg
     1021 ttggactttc attttaattt ttgtgaactg gtgactttgt aattgccttt ttgtgttaat
     1081 ttaaaaatgt cccacaataa cattacaaca ggtaaaaaaa aaaaaaatgt acagatttaa
     1141 attttgttcc attgtccccg atcttattaa atgtatccgt ccaggcggat gctaagctgg
     1201 tttgcgatgt ggtcagtcgt atggaagaca cagagccata ctctgcagag cttcttactg
     1261 ctatgaagcg tgta
//
LOCUS       KCVB01000172             760 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7858pri ultra-conserved element locus
            uce-584, sequence.
ACCESSION   KCVB01000172
VERSION     KCVB01000172.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 760)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7858pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>760
                     /note="ultra-conserved element locus uce-584"
BASE COUNT          174 a          181 c          198 g          207 t
ORIGIN      
        1 tctaccgagt ctacctggtc tccagaagtc agatggagag ctgcaccatc gacaagaagg
       61 acacacccct gctgaactgt gacaaacctc accaggacgt taagttcaca tttaagttcc
      121 aggagttcag ccccaacctg tggggtttgg agttcctcaa ggggagggac tattatatca
      181 cctgtgagtc actgtgtgtc acctcacagc tcatgttcac ctgagtgaaa ccatgtctgc
      241 tacctgtcag tgctgtatga gcatgtgcaa caccttaaaa tcagtggaac agagacacaa
      301 gctgctggtt cctacaggtg gaacagtgtt tgaatcaatg ctagcgactg ttttcttttc
      361 ttctcctggg aggaagagga cgccatcgtc ctcggctccc tctcattttg tcggtttgaa
      421 tgagaatcaa aaaatgtgac ctcgttattg ggccgctgtc tgttaaagca gcatcttagt
      481 ggcaagtgat gcatgagatg gagagcaagt ctgaccacgc agcctcatcc acctcgccgc
      541 gctctgacag cacacttcgc tctggcaggc ctgtgtgatt ccaagggctt acatgcccca
      601 aatcacacaa ggcttttaca gcatcaggca gatttgatgg gagctttcat ggcaacgtgg
      661 cttgtgggct gtgagatgtt gttatctgac ctaaatgctg ctaacaagtc tgtgtgtgtg
      721 tgtgtgtgtg tctgcatgtg tgtgtgtgtg tgtgtgtgtc
//
LOCUS       KCVB01000173            1425 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7897pri ultra-conserved element locus
            uce-916, sequence.
ACCESSION   KCVB01000173
VERSION     KCVB01000173.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7897pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1425
                     /note="ultra-conserved element locus uce-916"
BASE COUNT          419 a          270 c          308 g          428 t
ORIGIN      
        1 cagagaacag aaagctattc agtgggagag acaattgact ttggctgctt tttgtctcac
       61 agagccccag tgatgcggct gcagtgacta gatagtgttg tgccgaatag ctcgcgcaca
      121 gctgggttcc tgactggctc aatggcaggg ccactagtgc ttcctcctct aaagcacgag
      181 ggggaccggg aaagatgtgg ctattatcta agtagctccc aaataccctc catttcccca
      241 tcagcaaaga tgaaaagaag gagagccaga catggaggaa agggaaaaaa aacaagagta
      301 tacatatcag tttcatttgg aagggaaaat gcttttgtgc tgtggagata gatgcctctg
      361 caccggtgct gcctggtatc tgcacacacg gctcctcaaa tctgtttttt tgtacgatga
      421 ttcagtgctg tccctccatc tgcacacacg agcacccatc ttgcaaacgt ctccctcgct
      481 ccccctccgt cgctccaagc gttttctgcg ggctcctgcg cgatcaacag ctcataattg
      541 ttttttgcac atacgttatg caaatgagtc tttatggcaa ctggcataac aattagcatc
      601 ctccaacaat attttagtag gttaattgcg aaatttctga attgtacatc tgagttgtta
      661 attaggcatg acagaggtgg tgaaatagtt atcttcaggc ggtggcagcc agcagaggct
      721 gcttgggatg caaaaaggaa ggattgattg aaattagttt tcagcagtag aagcgtggct
      781 gttggagact ttttgtattc ccctttggct ttttgagact tctgtctgtc tgctgaatgt
      841 aaaaatataa cctgaagcag agagggaaaa gctgtgaccc ctgtagaaat agactgcacc
      901 tttgtgtcca tttgaaagaa attatgtaaa tagagacgtg aatgatacaa cttgctggaa
      961 gcataagtta gcaagccttt ttttaatgca tcatggggct gcacacttgt caaaacattt
     1021 aaagaccttt gacctgccag acatagtaaa accgaatttt ccttttttta caagtagaaa
     1081 tatggagagt ttttttttta ctcctttaaa taaagtttat tccatacatt tttgattatc
     1141 tttatagaaa atgaaaactt gggcctctct agtaaatttt ggcaccagaa aaaacaataa
     1201 aaatccatta acctgtgtta ctgaaacaca cagttgtaaa aattgaaatt aaaactttta
     1261 ctgcaccttc ggttatgaga aacaaatgtg ttataatgta tttttgtttg atagggctat
     1321 ggttaggatg agggcagtgt gcatatactt tcaggaacaa attatttgtt aaaatagtat
     1381 ttatttattt acagctgtga aaaaaaacgc tctgagattt tttta
//
LOCUS       KCVB01000174             979 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7953pri ultra-conserved element locus
            uce-953, sequence.
ACCESSION   KCVB01000174
VERSION     KCVB01000174.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 979)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7953pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>979
                     /note="ultra-conserved element locus uce-953"
BASE COUNT          307 a          231 c          171 g          270 t
ORIGIN      
        1 aaaaaagaaa aaaaaaaaaa tcaaattata ataaaaatgg gagtcagttg cctggtttca
       61 cctacagggc tgagggcttg ctcattattc acattattca ttctttccct gaacatgttt
      121 ttatgcaagg agaaattaat tgtctgctga actgtagttt aaagtaaaca agcggtgctt
      181 tcaatccatt tctaagtcgg agatggcaag cccggcaatg gccactttag cctgtggttg
      241 ataagtggtt attgtaggaa gcggatgatg cagtgatggc gaatgaatgg ctgcagacaa
      301 catcccatcc tcccagcccc atcataaaaa caacacttca tcttcttaac gtccctcttg
      361 gtgaatatga acaaattaac cactgactgg ttcactctaa ctttgctcaa catttttatc
      421 gtctttcatg tgcagatgac atcttagaca atctgcaatt taacccttaa gatgcagtag
      481 gcacagcaca gacaggatgg aaacaggctg taaactctta ttaatttgga tgtcaaaaac
      541 ttggagtgtc acttattggc tttgttgtat tttttttttt taatccctcc gctagtgttt
      601 tttcaatttt agaatctttg atccaaagtt tctatgggcc tcactaatta aaagatcaac
      661 atctaacagg tatgcctggt ggagacagca gcgcagacac tgtagtagtt aatttcccat
      721 actactcctg tgtggcttca aattgcagca attggatttt aatctgtgta ccactgatta
      781 gtcatttggc tccccagccg ctgtgtaatt tgttccacaa cagagagcgg aacaggcacg
      841 tgtgcacaca catacagtat acacagttga cagactgtct ctcacacaca cacacacaca
      901 cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca
      961 cacacacaca cacacacac
//
LOCUS       KCVB01000175            1687 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 7989pri ultra-conserved element locus
            uce-110, sequence.
ACCESSION   KCVB01000175
VERSION     KCVB01000175.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="7989pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1687
                     /note="ultra-conserved element locus uce-110"
BASE COUNT          579 a          292 c          302 g          514 t
ORIGIN      
        1 agagcaaatt aaaaaacata acttgtgtac tgtgtcactg actgtttatt atacactcaa
       61 tttcccttca ctgaaaaaga cagtcagttc attttcactg tcatattcat attcatatca
      121 aagttgaatc acatcaaatc aaagttagaa atgacagcag cttcttctct gtctgtggtt
      181 gttggaggac cacaggacag tctaagctgc tgcatcacat cctgagtgtc aaaacttatt
      241 gaagtggagg gaatgacttt ttgcctctat ctgcattatg gacagagcaa agagaatgct
      301 tacctgcaca taatttcatg agtaagaaga actctgcaca tttctggatt cttgtcctgg
      361 ccctcataaa ctatggcctt tgaaaggaaa tgagatggag agaaagtgaa aaacacagtc
      421 atctcttaaa cacatattag tttacataaa tttacaaata attagtttca catatctgct
      481 atttttgcac ttaataaaat taatcagggg atgaatacac atgattctag gacaacagaa
      541 tttattacat gggtgcaaag gctaagtaag aaacatggtt gaaataatca cagcaatctc
      601 taaattatat taattaatat aaacatcaat gacacttttc taaataaaca attacagaaa
      661 taactaagac taacaataat aactccaaca acaataattt tagtggcagt tatcataaaa
      721 aaatataatt aaaaaatata actgtaatga taataataaa ttgtattttt tttattaatc
      781 tagataatat cctgtggctc agaccgacag acagagaaag agagagagac agagagattt
      841 tcataaggag agatccaata gaagtgtcat caatatgctt ataatctggg gatatttggg
      901 ggaaagtgtt aatgggcaat ctgttgttta ttttgagccg ctaacaatga tctttctgtg
      961 gataaaaagc ggtgtctggc ggtacggttg agtaattttt gagcattggc tgaaacagat
     1021 ggcgtggagc tatcagttct gccgctcttc tctccctccc tcatccctcc ctccttccat
     1081 ttctaaggat agacacttca ctcgctcgca ggctatactg aaccggcacc aagtataaac
     1141 gcaggcaaag acacaacact attgacaaaa taactgagaa ataacacaaa caaaaataca
     1201 ttaaaataaa caacgtgtta ttacattatg ttttggtgcc attcatatat ttttttaatt
     1261 tatttctggc acgtttgagg ccaatatgaa agagtgacaa aaaaaaaagt aaagtgaaag
     1321 tgagaatgaa aaagtgacat actgaaccag gtcgcatcac tctctcgttt ttaaagctgt
     1381 actaatcgac ctaatgtgtg cattttattt tttttatttt ttctgggaac cgggcgatta
     1441 tctatattgg tttgatttat gtcgcaattt gcagctgtag aaacacaaat ccataaattg
     1501 cttgttctgc gtcattgact cggatcattc gcacaggaat gttgtctgtg accagtggtc
     1561 caggtttcac ggctctattc agaaggctct acctaaaccc tcggtttaag accagaaaga
     1621 gtggtggaga gctgaaataa tcatgtgtga ttttagaacc ataattataa taatgattac
     1681 aaatctt
//
LOCUS       KCVB01000176            1706 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8032pri ultra-conserved element locus
            uce-579, sequence.
ACCESSION   KCVB01000176
VERSION     KCVB01000176.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8032pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1706
                     /note="ultra-conserved element locus uce-579"
BASE COUNT          472 a          371 c          358 g          505 t
ORIGIN      
        1 accgggaggt gagacaaggc cacaaagctc agctagacag agtggctgag ggacagcctt
       61 gtccttctcc actctgccgt ggcagaatgg cttccagcca ctgctgacaa ttatcccacc
      121 atgccagagg gcagcttggg aagtcaggaa gaagtcacac actgttatgc cagagcagac
      181 aggaaaggct tgttgctttt ccagtatttc actccagcac aggacaggcg gggatgaagg
      241 gggtacaagg gaggatcgga gagaaggaag cgagagagga tgggtaacaa gtttgttttt
      301 catttggttt tcaattggcc attgtctgca tttaccagag tcaagtgctc cttgtgcggt
      361 ggacaccaag tgcagtggac actgagtacc gtctccatga agtagttgct aaattaaatt
      421 ttgagaggac aaacctttct atttagtgct gagttaattc caaagctatt tgccgttgcc
      481 cagttacaaa gatgaacagt cctcaacaac actgttaata aaccttatat aaaacatcta
      541 tcatccgtcc gtctttatac aaatatgctc taagttattc taaaatgtaa tgattaaact
      601 catgacatat gcgttcacat ggctcgggct ctctttagag atatttggct ttactcgtta
      661 ttctcagctt ggtcacacag aagacagttt tttttgaagc aaagaccttt tgatgatagg
      721 aggcaggcag cacaatgact tccagcggca cctcaatgca acagtaccgt atcacaatta
      781 gcagcgctaa acaggctgca cagacaagtc cactccatct cgtatggagc ctcatccagt
      841 cctctacgtt ttgtctgaca aatcccccca ttgttcctct gatgatgctt ttctttcgtg
      901 ggaaccctgg cttgtcttgt tgattagatc ccctttgtct tcctcccagg cagtgggcta
      961 gtctgcagaa tgtgaaacct cacactgtag tggtggctta atgcctcagc atactttgtt
     1021 tattaaacga tgattcagtc ttatatggta atgaatataa cgaagtttat ttaggtcacc
     1081 agccatagcg tgttgtggtt ttaatagaga attaatctat gcgtttattt tgaataactg
     1141 aattgatata tggttaactg ttttgcatta ttgtttataa cgacaagcag ctgttaaaag
     1201 atttaataaa aaacaaacat acttcagtca gttccttatt ttcctctttt gcccccaaag
     1261 gaggaacctg ctccaccacc agcaccatca ccaccaccca aaggcttcta tatctatgga
     1321 gatgttggta agatcacata tagaaaaggt gtttttatca tagaaataaa taacattatg
     1381 gtggttccta tggtgctaaa tctcaacaca atgtttgaga taaggacgga agttagaata
     1441 attgcttttc ctttaatccc tttaactttc aggcacaggg aagaccatgc tcatggatat
     1501 gttttactcc cgtgtggaaa acactcgtaa aaagagagtc cacttcaatg gcttcatgtt
     1561 ggacatccac aaaagttagt attatcctcc cactccacgg cactaacagc tgaaggttgg
     1621 cttgcatgac tttctggaag tttttgactc ctcatgtgct ggagatccta tatttatctc
     1681 tctgtttgtc ctgaagccct cctgct
//
LOCUS       KCVB01000177            1521 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8128pri ultra-conserved element locus
            uce-254, sequence.
ACCESSION   KCVB01000177
VERSION     KCVB01000177.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8128pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1521
                     /note="ultra-conserved element locus uce-254"
BASE COUNT          496 a          283 c          319 g          423 t
ORIGIN      
        1 gtcagcagct gcattgcaga acactatctt tataagtcca gtcaatgttg atttgagcaa
       61 cacaatgaca gtgttgtgtg tgtgccgatt ttcaaatgta ataagagtaa agaaacaata
      121 aaaataggta accttgaata catgaattac ctttacagtt gacacagtaa gaaggtttaa
      181 gtattctttt tatattcata tttgacttgt taatcaaata atttatttgt caatttgaaa
      241 actacaagtt gtcttttttt tttcttgaag ttggtctggt gcaaaaatcc cctgtgcttg
      301 catgccactc aatcactaca tcttttatga ttaatatttt tgtgtgggct taaacaagca
      361 taattataaa tgctaaacac tgaaaatgag attctaggca gatgaaattg ttggaggaag
      421 ttctgggcac agggttagag atgccatggg tgttttactg aaaagcagca ggagacggag
      481 ttaatgtggt gtatcactat ccgaaagacc tgctcctatt acaagccact catctccaac
      541 aaactctccc atgactcttt ctacctccat acttctattt ctatctgcct ctcttggtct
      601 cacctctctc catttcatgg ctgttttctt cctctatttg cttgacaaga caatggataa
      661 taaccctgta atagcacaga gactggcaaa tgagagtaat caagctgtct tgtcctggtg
      721 gtgttcaggt tcactcatca aaccctaatt atcaccctgg aaaccaatgt tccgctttaa
      781 ttgttctgat gaatgaaata ttgcagagag gagtcgggct gcagccgagc ggaataacag
      841 cttttagcca taaaacacag cagagtagga ggaggaggag gaggaggggc gggagtgaag
      901 gtgactcagg agagaacgag acattgtgca agagagctgg caaaagtaat ggataaatag
      961 agttcggtat gacagatgtg agaaaaagaa gaagagggaa atcggtggga aaaaagaagg
     1021 gttgagacaa atagaaaaat gacagcagca acgttcagag aggaaggcaa atgactcaga
     1081 ctgagaatgt ttaactgcag tctaattgct tcaagatagc atacaaggag gtgtctggcc
     1141 agtaatcgag acagattgca ctcagaggtt ttttacatgg ctcccagctg agttgttcca
     1201 gtataaaaaa gcactgaatt attgggcctc cattcccgta aaccgctaca cccctgcatg
     1261 gctccctatt gacaaagaac atcagtaatc aaatctcaaa tatcatatat caggggaaga
     1321 catcataaat tttaacaaaa cacatcactc attgactatt tggcttagga ttagatgttg
     1381 catccaaagt ttctttctct gaattgattt aaagatgtca catgcaaaac aaacctccaa
     1441 ataaaaaaca aaactagagg gcatgaaatg agacggttgc acacaacatc tgcctaaccc
     1501 actgctttgt gcattagaaa t
//
LOCUS       KCVB01000178            1740 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8174pri ultra-conserved element locus
            uce-68, sequence.
ACCESSION   KCVB01000178
VERSION     KCVB01000178.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8174pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1740
                     /note="ultra-conserved element locus uce-68"
BASE COUNT          509 a          309 c          408 g          514 t
ORIGIN      
        1 ctgtctgaac agttgatcta gataatcttg tgcagtctcc tttgcgtttg catgagagcg
       61 agagaaatta tttcagggcc tggttatgca gaatctgtaa tgagtatttt tgtttttttt
      121 taaaggaaat accggtaata actatgtgca aaaccattag aatgaatatt tatgctgctc
      181 gttatgaaat attcattaag tcggtataaa aaaagaacaa cgagggagac tggggagaga
      241 gattgtgtgt ttacttgtgt gtatttgtgt gtaaaatggg ggcggtgggg cccatcactt
      301 attgcacagg agtactgaca aaacccctgg attgaatatc tgacatctct taacaaaagc
      361 agcagcagta gcatcttcct ccccttgttt ttttgtaatt gtttcctcta ccatgtctcc
      421 ctctttctct ttctctatgc ctgaggtgat atgagttcct ctgttcatct gatcgccaca
      481 tccatccctc tttctccacc tttcattccc tcacttcccc ttcgttccat ctacccaaat
      541 taagaaatta atgagggata ggttctttac caactgcccc cgcaatgttt accactgatg
      601 aaattataat gtaggccctg ctgtattatt tataagccaa ttaggggaag tgttacttga
      661 tttgatgggg agccgacaca cagagtacat ttaactaatg gagaccaaga gagtaaaggg
      721 aaggagggga gggaggacag ctgaggcgga ctatttcgta cgatgctcta ggattacgtg
      781 gctaattgcc ctgctttatc tgtctgtcac ggatagccat gcatatttta gcgttcccaa
      841 agactttgat tgtttgcact cgtgttgggg ctgtaacaag tagagcagag gccagagtgg
      901 agtgtagtta aaaactggca gcaggggagg cagggagggg gcgacggaga ggaggggaga
      961 aggatggggg cggataggga gttggttggg gggttgcaca aaactcagac gctgtgacag
     1021 gtggagggag agttttgaca aggagcaaag gggtgtcgag gtgggaaaac gtagcaggaa
     1081 aaagaagtgg aagccgttca ttgagaagct gcaattagat ggcagcacaa acagtaatta
     1141 atttcactta ttgtagcctg tagccaagcg aggcttgctg acctcgtctg catattgtta
     1201 agaaacaatt tcataattct gtttgctcat ttgcatggct catttacaac acaccatggc
     1261 aaagtagtga catccaagga ggcagcatgg gacaaaaaac ccagaaaaag ccagtgcaca
     1321 ccagcagctg gagactggga aagaaaaact catgtacaca tagggcagct gaatgaaccg
     1381 attacttgat ttatcgatta gctaattcaa aattaatcta gattcatcac acatttatta
     1441 ttattattta gaaagaaatg acaagacatc agtcttttat ttaataaaaa gtgaaaaaat
     1501 tgacatgttc ctgccaatct aatgagaaca cttgcagctt ttctatgttt catctcagtt
     1561 gaacttagtg ctgaaatgat tagttgatta attgattact tgattgacag ctaaattaat
     1621 aaaaaaacaa ctggtttcaa tttctcaaag tgaagatttc ttattttttc tgctgtacag
     1681 tatatctttg tatttgaatg tctttaggtt gggaaatact ggttgaacat tctgctctca
//
LOCUS       KCVB01000179            1524 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8214pri ultra-conserved element locus
            uce-2, sequence.
ACCESSION   KCVB01000179
VERSION     KCVB01000179.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8214pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1524
                     /note="ultra-conserved element locus uce-2"
BASE COUNT          470 a          324 c          299 g          431 t
ORIGIN      
        1 ttgcagaaat aagcaaggaa ttatatatag gattgcaaag gagctatgca gaaaaaatta
       61 tgcacaaaaa gaaacaatat taaatattgg tagaataatt aaaatcaaat ttaaccattt
      121 tttttatcat caaaatagat attttatcag atttttaagt tatattcaat attgtcagta
      181 cttttcactc aactactttt taccaaccgt gatctgcaat tgatctgcaa taacatcaac
      241 gtattacctt ggaggtcagg ataaagacag aaagatgatc ttgtcattct tacatgtagg
      301 ccccgccctc ttaggatcaa cgataattta ataagaggac agtggctttt gttttgttat
      361 cttctccgca cctgtctgct tataggtatg caaaggcttt ttctctttta ctgtatcaga
      421 acgaaaatag gtctatttaa tagatctaat aacatcaaac tgatgtgaac taaattgtaa
      481 atgtaaaata ttataaaaat aatattaaat ggcaagtaga tctgcccccg ttaactaagt
      541 ttttaacact tcaccctatc tgctcggcac atttagtcac ttgcgccgct tagcgtcctg
      601 tcagggaaca cgcaaggcgt gacagatgac gtcgcctgtt tacaactgcc aatgaacacg
      661 tggtgctccg aatcttgaac tattattggt caatatggcg gtaacatggg cggggactgt
      721 tcgcagaaag accaatagcg tttggcatgt aaattccgtt tgaaactttc ccgggaaatc
      781 tgagtttcgc gggagggctc ttgagcgcgt aaaccgtatc ccttcattca tcaggccgtt
      841 tcctgaagcc atttctagtc gggggaaacg agagttgcgc tactttttaa tccgttgcat
      901 ctgcctaaat gcacattctt aaggaaaaat actttacaaa agtcgatgaa tcagactcta
      961 atctttactt tggtctgaat atttttgtta ctattgcggc ggattagcag ccatacgggc
     1021 tagaaagtac agagacaaga aaggggttac cccgaatacg caagatgaga agagggatct
     1081 cctcagctcc ggacaaagtg attttagcgg gggttggggg ctctcctctg gacaataata
     1141 taattttaac aactctatcg gatcgtttaa accctggtca atccaacgct acgtttattc
     1201 aaataataac caccccaccg ccttgcaacg ttacacagac atcaaatgtg tgtttatctg
     1261 aacctcagat aaacaacatt tacacaactc cacaacaagc tgcagcaaac ggagcaggac
     1321 aacgacccgc tctgggaaga ccgccggtaa tcatctatac agctcgtttg aacattttga
     1381 catgctacaa ctagccacaa acacgacaaa atgttagctt gctaggctag ccgagcttgt
     1441 tgttcgggca gctaacgtta gccacttagc cagggcattt ccacggctca acctagcgag
     1501 gaggctaact aactaccgct agct
//
LOCUS       KCVB01000180            1593 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8257pri ultra-conserved element locus
            uce-704, sequence.
ACCESSION   KCVB01000180
VERSION     KCVB01000180.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8257pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1593
                     /note="ultra-conserved element locus uce-704"
BASE COUNT          439 a          303 c          347 g          504 t
ORIGIN      
        1 ggaaaaccat gaattcctca atccaattgt gggtttttgg gcagtgcagg ctgcagaagt
       61 agcagctggc ttcagtaatg cagttataga agcgacctgc agggttgggg tcaggccatg
      121 tagactgcgt gcgtgtgtgt gtctgttaaa gagagagaga gaaatggacg ggtgccttgg
      181 cgtttacatt cacccccagg cactgcagct taactgggct gctgcaggtt gtttttataa
      241 ggagcctctt gtgttctggg gtcctctggt ctgctgtctg catgtgtgtg aagctctttt
      301 actgggtggg aggtcaaaga gggctaattg gacaagagcc tgtgacttga aacacatttt
      361 attaaagaag tctcaaacat ttggatgtga ttaacaaggt agtaaataaa tcggtgcaga
      421 agatgattga ctagagcata agcatcctca ttaaacttgt gattaagctc atccaaatgc
      481 taatatctta acccaaatta acatcccagt gttgattgtc tgcagcggat gcatattcaa
      541 cattttctct gaaattgtcc cagtgactga cctggttggt acctgctgat ccttggacag
      601 acagactgaa ctggcgagct ccagtaatca gcacgccgtg gatttgttta agattaccgt
      661 attaccataa ttaagggctt ataatagaat gtgctaattg catttttggt ggcaagggca
      721 tgtttcttaa tggctcatta gacccacagt acattggcag atgttttctt ctgagtagag
      781 gctcactttt cagtgtccat gataccccag aaaacatcac tggactacca gctaataagt
      841 gatctacact tgtcagagac tataatgtag ctgtgtccat ctgttttact ttggaaaggc
      901 gaaagctgtt tacctatttc aaactaacct cagtcaattt cctgtcattt aagtgtatct
      961 tcttgccttg cccagtttca tttgtgtttg tgaatagagt ggaaatggga ggcaatttaa
     1021 tctctgatgg gctaagttaa ttggatttga attatttggt tttgaaatgc tcaattttgt
     1081 gcattttgtg tttgcaattt gaatttgtaa tgaaaagcta taaaaagagt caaaaccgga
     1141 gcccagaccc ccatttaaac aattgttttc aaaaaaagtt tgtgacagag ctgtaagaag
     1201 tcaccttcct cttgcacctg ctgcatttat gttttttatc tctaggatac accaaccatg
     1261 cctgtagcag aataaaagat caataaatat tgctgtatgc attgataaaa aacaattatg
     1321 gccctttatt tatggaacct ctattgatct gtcagcataa aatattattt ttgttattag
     1381 tcacccactt ctgcatacac tcttcaggtc actccacagc tttgagaggt gatgtgagcc
     1441 ttaggtacag acttgataaa cccagagaca tgccctaatc tcactaaatt tgtttttctg
     1501 tcatcatcac catagggata aaaattacct gaactcgcca ggaacttgat tagactgatt
     1561 ttgtttgttt ggcagctgcg tcacctggga aat
//
LOCUS       KCVB01000181            1173 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8300pri ultra-conserved element locus
            uce-390, sequence.
ACCESSION   KCVB01000181
VERSION     KCVB01000181.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8300pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1173
                     /note="ultra-conserved element locus uce-390"
BASE COUNT          305 a          257 c          270 g          341 t
ORIGIN      
        1 gtgctactat aaaaccctta ctcagtagcc ttgctggtat gatctgtgct ataggttggg
       61 cggccaagta acatcggtca ggcgcaaccc atcattgacc agctggcaga ggaggcgcgc
      121 gcctttaacc ggatctatgt ggcgtctgtc caccctgacc tgtcagacga tgacatcaag
      181 agtgtgtttg aggccttcgg aaggatcaag tcttgcacgt tagccagaga ccccacgtca
      241 gggcgacaca gaggctttgg cttcattggt gagctggagg gccctcattc gctcgtgctc
      301 tttgtgttac ccttgctctg ctattaacta ctattttatt cacttgcaga gtatgaaaag
      361 cctcagtcag ccctggacgc tgtgtcttct atgaacctgt ttgacctggg gggtcagtac
      421 ctacgggtgg gcaaagcagt gactccgccc atgcccttac tgacccccac gactcctggt
      481 ggcctgccgc ctgctgcagc tgtggctgca gcggcagcca ccgctaagat aacggcccag
      541 gcaagtatga atcccttcca aagggattta atggccttcc aggtaaatat aacccagatc
      601 tagctagact tgacaccaaa agcacagata ttatgggcaa agattttttt taataaggtg
      661 actgggagaa ggccagtttt gtctgaatca cataaaacag tagttgccct gacatggtca
      721 atgtaacagc tttaaaatat gagacattct gggcttaatt tttttcccca tttcaatgtg
      781 ttcgaaacac aaacagattc cgaagatttg attcatttta tatagcattt atagtatagt
      841 aaagtaagtg ataaaagctc ctcccattca ctgattgggt gtgtaagcta agagtcttct
      901 ctcttagtta gcacaaacac actgacagct aatttcagtg tgaacagtta caatgattaa
      961 ccaggcagat gtgttccttg tagaatgtat catgattgaa aagtttctga acataactgc
     1021 tgtatattta tcacttatta agaagtgtca atgatttttt tagtgaatat aaactgaaat
     1081 gatgttggtt actttgaatc ttactaaaga taggcgaagt tgatcttact ctgtgcaatg
     1141 ctgtgtgatc gttgactgtg ctgccttgtg ctg
//
LOCUS       KCVB01000182            1437 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8364pri ultra-conserved element locus
            uce-405, sequence.
ACCESSION   KCVB01000182
VERSION     KCVB01000182.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8364pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1437
                     /note="ultra-conserved element locus uce-405"
BASE COUNT          398 a          338 c          298 g          403 t
ORIGIN      
        1 ttcactcctg cagaattggt tgtggccttt attgtttcgc ttaacacact tcagtgtgca
       61 gtgaaacagc cgcgacatgt aaaagcctca aaaacagctt tttttctccc cccttctgac
      121 ttcacccctc gcccttgacg aatgtgctcg gctcgcaggg tgcaactgtt ctgctgaatg
      181 gccctttcct gttgcttacc cttaaagtgc aggcagttgc cgaggtaggg ctccctcccg
      241 ccatattatg ggattgaatt gcgtttgacc tttgtggaga acaatggctg aaggcctttc
      301 atttttctgg ccttctcagc gcctagaaga aggacctctt tactctgtgt tttcactgtg
      361 gtctgccagt aattctcaag caggatgaca gggtgatcta tgctcagtaa gtgtgtgtgc
      421 gcgtgggttt atgtacacaa ggctacacac aagtgcattg tttgccaatt ctgacttgta
      481 tctactgaaa ttactgttca taggtcaaac aaaatataaa accttaagat agaaccgttt
      541 tgatttgact atattttggg ggccacccaa tttggctacc caactgcact gtcctggaat
      601 aaaaagaccc tgtgaagaga agagtagttc atagaagttc agacacagct ttgctaaaga
      661 ggccacagca gtgagcggtc ttgccctgaa tacacgataa aaggtttact gtaaattcta
      721 aatggcggtt tctgcaaccc atacatcact catgaccatt aggttccacc ctcactgcac
      781 agtgcaccct tccagccaca gctttccaga tgatacagta cagaggctgc agttcaggca
      841 gatctgcctt tgaatagcct gcagccaatg gattttctct ggataataca atgtttggga
      901 cagagaggca gggattagtc cagacggata catccaccac cccccacatg cccaatctaa
      961 ttagactgga atcagtttaa gatgaaagca agccctagca tcaccgcact aaatactgga
     1021 attagtattc cccgcatggg aatttaatga cggagactga aaatccaatc acattcactc
     1081 ccagtttagc ccctcatgca ttgggcctgg acaatgacga aaccaaagaa caacaacaat
     1141 gatttgtttg tcaatagata ttgaccaact tcacatctga ggccacttgg gtattggcgt
     1201 tactatgttg tcatcattta acgaggataa acagctcata atcagatatt tagacagtct
     1261 gtttctgaca taaaaccttt gtcacattct gactcttcca ggagccataa cagaaattaa
     1321 tcacaacaga aatagcaagg aagatggagc attcaggaca attcaggaca agcacagcca
     1381 ttgtcatgat gtgtagtttt gagtttagtt acctcctgtt ttattttgtt aaccctt
//
LOCUS       KCVB01000183            1357 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8404pri ultra-conserved element locus
            uce-235, sequence.
ACCESSION   KCVB01000183
VERSION     KCVB01000183.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8404pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1357
                     /note="ultra-conserved element locus uce-235"
BASE COUNT          396 a          255 c          254 g          452 t
ORIGIN      
        1 gcaattattt tcttttgtaa tttttgataa tgtttttttt tgtttgtttg tttttgtttt
       61 ttttgtcttt tagactagtt gatcagtcta agtttaaact gtcatttaaa tgtcagggaa
      121 attagttttt tgaagcagct ctacttccat gttttctaaa gtacagtatg catgtatagc
      181 aaggtatgaa aaaaaacaga ttttctagat tatcaattta aaactaggga ctgtatttta
      241 tttttccaca aattacacca attcctcttt gatttatttg accaacatgc atctaaatgg
      301 gaggggaacc tgggagattg ccccacgctt gttctttgaa ttacataatt tcttccggct
      361 cagacctcag tctgacatta aagaagaact cagtataaat ggataaaaaa aagtattagt
      421 attcactgca tgtatgtgtg gcacatgtgt gctattttta catagggatg ctgtgaaagt
      481 gtggaattgg ggacaaggga gccttgcacc gggccgtgac acagacttat gttaaaccta
      541 attagatcat gataatagct ttagggcccc agtgatcaat gctagaggtc tgtggagatg
      601 aactttcctt aagtttcatg ctgcacgctg gttgtactca ttttttttca tacacagaca
      661 cacatacaca ccacacacac acacacacac acaaaagtgg ttttagggcg ctgtgttata
      721 tgatagcatt atcttaaata tggttttgca tattggtttg ccattcttgt gttgtgaatg
      781 agtataacat agctagaccg atatacatat ggtcaataac ggactgacta caattttaaa
      841 tagcctgcat cagagctaca tcctccgcca ataagatagt tacgattcag aattctttaa
      901 tatttattca taaagcctgt ttgacacagc aaacctccaa gcctctccca taatggaatg
      961 cacccttagt tctttacatc actgaaagac acttgctgat gttaagctca aggaccttac
     1021 agcgaagtct agtttacagg ttacaggctc atttgatcac tctcgctgag gaccaagagg
     1081 attccttatg cttccattac tatataatgt aatttgtgta gtataatatt tcaacatgtc
     1141 tgcgtctgct gacatgctag ctagattgtg ataagtcttg atactggatg ctttgggaat
     1201 ccctgcacat atcggctgta attgtacagg ttgttgacaa agcttccttg ttctgcctgt
     1261 ttctcccaac cccaactcag caagaggtgt ggaggagcac attcaagaga caaaactgta
     1321 aaaacaacga gttttgaaaa gtcacccatc catttaa
//
LOCUS       KCVB01000184            1282 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8491pri ultra-conserved element locus
            uce-1242, sequence.
ACCESSION   KCVB01000184
VERSION     KCVB01000184.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8491pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1282
                     /note="ultra-conserved element locus uce-1242"
BASE COUNT          311 a          276 c          384 g          311 t
ORIGIN      
        1 cggactgagc ccctgcgctt aattgggcac tcaaatgtta ccaggtccct tcccaaacga
       61 cttggagggc acacatggcc aagtgaggga gtggagagaa gcttgtggag agaggaaatg
      121 gagcagaggg tttagcacct cctgcggcca agttttttct ctccttctgg gtttttcggt
      181 caggacggcg gaccgcgtga gtgatagtgc atttggcgtc tcgctgtgcc aaggatgaca
      241 gagcaaaatt aactcctctg tggtggagac agatggaaat gaggctagtt tcactttcag
      301 ttttgcttct gttgcagagt gaacacattt atctgttgca gaggcgagtg caatagtgga
      361 aagaactaca tcatctcata aaaaatttaa tctctaaaat aataaatctg tgctttaaga
      421 aaggctgtaa aatgaaattt aacaccaact cgattatttg atcacatgga aaagcaggaa
      481 ataaactgca gtttttactt aaaaagacag gacttcaaac gtgtactgga gcaagtaggg
      541 agcaaattca taattttttc cagttcatca aagcctccac cggctaatcc ctttcttata
      601 taaatagctc ttttggtgga gagggtagct gccgcataga gctctaaccc ttctgtccct
      661 gaagctacag ccaggataag ctgtgtgttg gattggcaca tcgggctgcc tggggcctgc
      721 tgctgcgctg gccgccagta cagatggagg gcagggctgg gcaatgccag cttgtcagtc
      781 ggattaaaac gactcattta ttcactgaag tatcgtgcaa agcgaccggg ccaaagcggg
      841 gtgaggcgaa agggggagag gagggtcgaa cagcggcgag atgggggcag cttggatatg
      901 agagagggtc ctctgctctc tggccctcag ccctcccttc ctcttcatcc cgctctctgc
      961 ctttttgcct gttacttatc tttctagggc tgccagaagc cattaccgca gtgtacggaa
     1021 ggcggccggc agggtggcat ctcctagtgc ccggtgacag attgccgctc ggcattggcg
     1081 gcggaggagg tgaggggtgg aaagtgattg gctctgatgg tcaggaagtg ggcactgagg
     1141 gctgtagaag gaggtggagg agggcagaga gtcagggaga ggatgacaag acctggctgt
     1201 gtgacctttg gtatcgctcg agttacttaa aggggcacag gccctgcttt taagagcatg
     1261 cttggcagtg gtggccgccg tg
//
LOCUS       KCVB01000185            1721 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8532pri ultra-conserved element locus
            uce-932, sequence.
ACCESSION   KCVB01000185
VERSION     KCVB01000185.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8532pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1721
                     /note="ultra-conserved element locus uce-932"
BASE COUNT          584 a          308 c          334 g          495 t
ORIGIN      
        1 ttatcttttt ttatccacct ccctacattt gtagtttgag tgtaattcat taactaaaaa
       61 agagacaaaa cacacaataa acaaaattaa agataaattt gactttaaaa aaaattctaa
      121 gtacattgcg cttaggcaaa ttgtttaggc attaaggcaa atttattctt taattttgaa
      181 aaaatatact ggatgtttcc caaaaattta gttgtcattt attcagacac atttgcacca
      241 caagataata aattatatta ctgaatcacc atttggccca aattgaacca actaacatta
      301 ataagtaaac gccaaaaata tccattatgt ctactgtctc atgtcaacta atgctcttac
      361 taacaattcg gtttctgaat caaaaaatta cgaaaaattc tcagaaaatt gatgtttttt
      421 gattcagaaa gtgatcaaac ctgaattttt tgaaatggat gaatgtgaag tcgacacagc
      481 catgcctttt taaacctgaa atgagcatca gtggggatta atatgcatta attaaattga
      541 ctctgtttcc ctttaatgag agggaaagga tttgatgtta atgctgttga tctcacgaag
      601 gcttcaatcc acctaattaa ccagtattcc tgcaacacac taaaataatc aataaaaaga
      661 gatatatttc atcactgcta tggtttgcat gctcagatat tcagataatt aaaacacatc
      721 acatgctagc tcaccttagc aatgacagaa agaggccttt cagtttacaa acatgtttct
      781 tttttggtgc aataattaaa aatgcctgtt tttatctgag cttttagtca tagtgtggca
      841 gcactcactg ttgcatctgt gtgttgtgca gagatagagc gtctgtggag ggccagggag
      901 cagactccag agatccatcc atctcaatct gaggctacag ctgacacctt acaaagaggt
      961 acaattaata agggcattat ccagcagtag gcaacaatag agtcctaacg cagagacacc
     1021 aacctcggga ggcccggaga ggagagaaag ggagcgtgtg tgtttatggg tgggggaagg
     1081 gtgagccaaa gtgcttcaaa atgggatgca ttacaaccaa tgtcttcaga acatgaatta
     1141 caatctatta taactggttc ttatgataga tggtaccagc tgctttgtgt gctaaatggg
     1201 tcacttccag cagctcatcc tgcacatcct gcatttaggt aatatttttt aaaaattctg
     1261 catctaaaat ctaatatttc caaaataaaa tgcaaatgat taaaataatt cacattttct
     1321 gattgtataa agccccttaa ggtgggtact accaaattta gggaataaag agctgaaaaa
     1381 agtaaaatat aaaaaggcat aaaagacaga ccctcattgc tctttgtttg atgacatgtc
     1441 gttcacttta tagaggaatc ataaggagga aatctgagat atcaaataca gcataactga
     1501 taatggagct ctcacgaaag tgtcccatta gtgcaggttg agtgcagccc tatggttcac
     1561 aggggagagg gcggagaagc cgctgcactg acatttacag caatccagga gttgtttgtg
     1621 gtcactggac tggagacaaa cgctgaaccc aaaggtcaga ggtcggggct aaatttcatc
     1681 cgcagtggag gtccctctgt tggagaagac aaacagagaa a
//
LOCUS       KCVB01000186            1225 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8572pri ultra-conserved element locus
            uce-171, sequence.
ACCESSION   KCVB01000186
VERSION     KCVB01000186.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8572pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1225
                     /note="ultra-conserved element locus uce-171"
BASE COUNT          370 a          253 c          262 g          340 t
ORIGIN      
        1 taaccatgtt acattaacaa acctgctaaa aatgctttac ttttatacaa atgtcacaaa
       61 gtagtttaac ataggctttt tacaaacaag ctatttttgt tacagcctgt attacctgtc
      121 agaggagcgt atattcatgt gtcacataaa gagaggctta tgggtatcta tgaaagacaa
      181 tgaagaacac agaactgtag ccttatttag acaaaaaggg tttgctggga tatcagacat
      241 catcgcgtga cacttcagtt acagaccgag ctgttgttta tacataattc ctttgtagtt
      301 tcaggagctc attctgtggt tgatcatatt ctcttctgtc tgttcagaag ctgacctgtt
      361 gtgcagagat gtgcatattt actctattca taggctccac acagcacaca tgctacaggc
      421 acatattcat tactaagtag cctgtggcga ctgtcatgtg cagacaatgg tggaagacaa
      481 aaacagtgta tctaccacac agggaatctt ccacacttgt agccacacga cgcagacagc
      541 gtgttttttt aatcatattc acataatctt gtgagagtcg tatgtggaat cacagataag
      601 cctccttatg ttgctcagat attactgcaa atgagcatgt ctgagaaatg taacaccttg
      661 cacagactct tgcattttaa tttctgtggc atctgtgacc agtccgtcat gtccctaacc
      721 ccctcggggg acccccaacc tatctgctgc agacccccaa aacccaggag gaggggaggt
      781 tgggttggag gggggggcag ggacacattt gattgatgat ggtgtgaatt aaacagaaag
      841 agatggagag aatcctttta tcaggccaga aacacgagtg gattaaaatg tagttgtaca
      901 acccctctgt ataatgcagc agcctggccg catatcacga ggcaccagaa gccaagatga
      961 gagctgcttg ttattattgc cccctcctca gcccaccaca tgaaactttg ctgaagcagg
     1021 aaaaacaggg ctgaaacagg tgaaacaggc taaaaataga gccgaaacga tcagcggatc
     1081 aacaggaaat tcatgtaaaa atcttttaac aatcatgttg gtcaatctta aagccaaatg
     1141 tgagaatttg ctgcttttct ctttgttatg tttaagaagc tgaatatcct tgtgttttgt
     1201 actgttgatc agtcaaacaa gacaa
//
LOCUS       KCVB01000187            1902 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8614pri ultra-conserved element locus
            uce-977, sequence.
ACCESSION   KCVB01000187
VERSION     KCVB01000187.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8614pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1902
                     /note="ultra-conserved element locus uce-977"
BASE COUNT          539 a          485 c          397 g          481 t
ORIGIN      
        1 gaaacgactg taaaacatgg aagctttaac taaaagctta cccatatgca cagcgggaat
       61 agagaagtga ttgcagtaaa taatcttatg tagcaagccc agagtgtgct ctggtcatgt
      121 ccgtaactct tgcaaaaccc tgcaaccttt cacaagcacc cctctctgca agcctccctc
      181 agtcactggg aacctgtgtg ggtctgttgg ggtgagctga cgggctcata gttacgacat
      241 ggagacaatg ggagggtcta atgaggaaca tatgcttttg tgtttcaaca gagccgctca
      301 ttgtgcaagg aaagggatac tctactccta ttcagactcg tcacaatgaa aatccgagcc
      361 ctccctgagc cagttgagct tacaatatcc atccgcttct tttttccctg ttcttttttt
      421 cccacccctc tttcttgact ttaattctcc atgcattcat tttgcttatt gtacttatgt
      481 tacatgtttt gtcctacaga ttgaagaaca atagtgtgtg aaactctaag cagtaaaagg
      541 cagcagagca acacaaagca aacataaaag cagcggaaat taggcaaagt agacgaccca
      601 agttatatgt taatgtttgc gccttcgtcc cttcactgac aaatgcactt gactgataaa
      661 ttaagcaatt cacttggtgc cagatatagc agaaaaagct cttgacctcg tcctaaacac
      721 ctcagtactt gcacggtggc tgtcaagcat aaaagcctaa acaacatgca aacagcatgc
      781 gtgcatatga ggacgtgcac gctcgtgtgt gggtgtctgt ctgtcagtct gtctgcgggt
      841 tttgtgttct cgtctgtgcg tgtatcttcg tgtgtctgtg tgagcctgag ttcagacagc
      901 agtagctctg gtaatccatc caggctccca gggcagccag ctcctacttc ctgatgcatg
      961 agaagccgct ggagtggagt ggcagcaaca actgctcagc cctatttgat tactaataag
     1021 agagttgtcc cctctgtcag ataccaggca ttctccacta agcaaacagt gattccggct
     1081 agtcaaacat tagcattgct gtctctccct actacgtggt gagagttaca ttgagatcga
     1141 cgctcagact aaacccccta cctcacccct atatccaaca cacacacata cacacacaca
     1201 cgcaactacc taaacatatt caaactgacc gggacatata gtttcacagt gtgagaaaaa
     1261 tgtcaatgcc tgagaggagg ccatttattc tccctcttta gaaagtgatg aaggtaatat
     1321 aaatgtggca gatgtagctt ccctgaaggt gcagaacaaa acagcagaca taaaatgttt
     1381 atcccttcta aaacgaaact ttaatggcat ctagctcaca gctaacttga gagaaggaaa
     1441 cagtcaatga aaaacaacta ttgacagtgt tagcatcact gtcgtaaaca aagaagggaa
     1501 gccagccaaa taaatgtgaa gtgcctaagc taattaggtg tattcccagc atgatcctct
     1561 agtgattgaa ccacctgtga ttgaacaaac cctatccagg tggtgaccgc ccctcggtgc
     1621 acaggtacct gggaaagacg cccctcagcc actcacacag aatcacccac agttcccaca
     1681 ctgtgggcta taagatgcaa gtcagcccaa ctgggccgtc tggcacacca ggagggaact
     1741 catttgttca ctagctcgtc tatgtgcccg ctcatccact cagtcactca gacactcact
     1801 cattcactta ctcgttcccc cccgttcaca aacacagaaa gaagccgcgc acagcctacc
     1861 tgtcaatgat cacagggtct gatggagtct cgttcctgtt tc
//
LOCUS       KCVB01000188            1333 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8655pri ultra-conserved element locus
            uce-134, sequence.
ACCESSION   KCVB01000188
VERSION     KCVB01000188.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8655pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1333
                     /note="ultra-conserved element locus uce-134"
BASE COUNT          391 a          282 c          253 g          407 t
ORIGIN      
        1 cctgttttcc acccttgagc tgaggccaag ggagtggacg cgccggccct gtgaccagtc
       61 actcggaaat gtcattatga acacagtatc attattatca aaacaaggga ctggcaaggc
      121 tgaaagtgga gccagttgtt tgcacaggtg tgagtgtgtg tgtattcatg catggctctc
      181 atttgtgtgc atgcattggt gtgttttccc cgtgtcttaa aaatgccttt cttgcagaag
      241 attacaatat aaaaccaaat ttcaagaggg attttgcaat tagtgagact cactatttct
      301 gattaaatct cacatgctca ttatctacct cttttttatc ttcctcttcc aggtaaaact
      361 acaatctctg attgtatcca cgagaaaatg aatcaaatgg cttttctggt gcagttctat
      421 acagaggaaa cacaccagct cctaattcca caaatttgta caacgcaccg tgacgcaaac
      481 acataaaaca aggatgctcg ccatcgtctc tgccttgaat taaccttctg catatgttaa
      541 actgcaataa caggtttggc tgtcgtgtct cgcctccctt tctcactgat ttaactccac
      601 attacgccgt tccacggggg cctatatccc tgcgtgttaa tcactgctct tcctggcgca
      661 cagagaaata tatggcgggt gcttgcagag gaacagcgga ggacaaaaac gtgctcgctc
      721 ttggacacta cttgattaca gactaattgg tctagagtga ttaaatatct catgcttctc
      781 gctaattgca gctatttaaa gcagctatta gcttaaaatg cctgccacca acagccgttt
      841 actcataaac aaaatgtgag ctgcagcaat tcacctgtac tgctcagagt ggaaaacaag
      901 agttagagac tcgcaggaag cttcagctac agggagacat caaagcacat cttaaatatt
      961 taaactctta tttttcagaa agcaagtgaa tactttatgg aatactctac attgcttttt
     1021 taaaaataca tttcgaaaaa tgagtagcta tccatctttt ttcccctcct gctattccaa
     1081 ataagaaaca gatgtacatt tttactgatg accaattaat cacaattata ttttccacat
     1141 ttatattact tgtgataatt gatgtgtaat tatttcttca tttgggctgt gtgtgtgtgt
     1201 caatgatttg acacttaatg tttttatata tgagaggaaa atatgaagaa acaataatcc
     1261 cacaaaggat cattcgtttt aatttaggag cctcagccga cgctttgcac tcagagatac
     1321 ttgaggaaga agg
//
LOCUS       KCVB01000189            1460 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8697pri ultra-conserved element locus
            uce-619, sequence.
ACCESSION   KCVB01000189
VERSION     KCVB01000189.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8697pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1460
                     /note="ultra-conserved element locus uce-619"
BASE COUNT          486 a          277 c          307 g          390 t
ORIGIN      
        1 tttgcattat tcagtcaatc actcacagct cagcctgaat tcagtttcag gcttttcatg
       61 atagaataat gaataaagaa cagtttcttt tgacacagag ccaagtggca aattactgca
      121 attgctggct acagtggaaa tttaaaacaa agccaacatg attatgattg atgttgcagc
      181 atgaaaagag gtaagtaaag tgggccacca cttctaattg ctagaatgaa ggcaaactaa
      241 taacgtgtct gctacaagaa gcaataaaaa gcttttaatt gaatctagtg accatcagta
      301 aacaatgata atataaattg ttaattcaat tgagggttat attgattgct ttattaaaat
      361 aagtcagtaa gataaagctc ttgggaactg atacaacacc atcttgcagt gtgtgcactt
      421 tcagaaaagc gacatgggcc ctgacttcaa gtcagtgtaa aacattttaa aaagggtaat
      481 tagttaacag ttaaaggcag taatcaccta gtgatgaaga taaaaggact caaaggaaaa
      541 caacctggaa atgggctggt accattcaca tcacagtcgg attctagagg tcatgtgcta
      601 agtcctgtta agagcctcaa agggcaaacg ctgagcactg agcacttttg tgtgagtgtt
      661 tagaaacaca ggcagatttt aagcacacaa acatgaaaaa tatcaatttc acatttggtt
      721 taacgctttc acactttgtg tcactgacac caagagagaa gggctgtgga agacaaaaca
      781 cagctcctgg ataaggatca tgttaatgcc tatcaattag gatcaaaaag ggattaactg
      841 gcatggttgc agcggtagga tgggggccca tgcaaagtca ctgaatacta ggggtcacac
      901 ccctgacttc attaggcctg acctttccac gccattgtgt gacaggaaag agaccctgca
      961 gtctgtgttt ctccttcacc catcttagat ggcttatgga gagtcaaata tgagacatgc
     1021 agaaaagctg ttagagatca gtggaatatg aaatattcac attcaaagtc cagggagact
     1081 tattaggtta ctgcaacttg gtgcaggtgg gtatttactc atcaaccccc aacctccctg
     1141 caaaggaaat catttttttc accttagaga ggagtgagat ttactaatta gtcatggagc
     1201 agcctcccca cgctatgatg taccgcaggg ttgacgagct ctaactcttt cttgccatct
     1261 taattcacaa aaggtcaaat ggcaaagtga aagcaggtat tccaggtgtg ttaaaataag
     1321 ccccaattag ggaaaaatgc aagcacaccc tcaagtctca catatccaac ttggaaggaa
     1381 actttcagta aagctcctaa agtggggaaa tttaatgctg tggcatgtta gagtatttct
     1441 tcacatattc attacataaa
//
LOCUS       KCVB01000190            2137 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8740pri ultra-conserved element locus
            uce-1106, sequence.
ACCESSION   KCVB01000190
VERSION     KCVB01000190.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8740pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2137
                     /note="ultra-conserved element locus uce-1106"
BASE COUNT          440 a          559 c          488 g          650 t
ORIGIN      
        1 ctgataggcc caagctcggg ggtatgtgtg ttaacagaga aacagctcca cagttaggct
       61 tattattttt ttaatttttt aatgcagttg gctgatatgt tgttgaattc acagctctgc
      121 acacagaata tgacataaaa ggcagaatat acggtctggt taagcttact gctgtttcta
      181 tctttccatg atcgatgtca ttgctgtcat tgttctcaat cccactaatc ccgccgctga
      241 gcctggctgt ccaaattggg tgtgctgggt gctgcggagg ctgaggaggg ctgaggagag
      301 tggggacaga agcaaagtgc acagaagctg atgaaggttg ttgccatgct ctgaagtcga
      361 gcatcatctt ccccacagtg ttgggagtgg atggggtagc tatcagtctt taattgggca
      421 ctgtgggcca taataaagag atctcagagt atggagaaat agaatgaggg agaataagag
      481 aaagatactg agtgatatgg aaggccaaag agtgggggtg aacctggtgt gctttaatgc
      541 aatgcgtatt ggcaggtgtc gctctgccta atttcacgcc tctgtgagcg aatgtgcacc
      601 cagacgatcg ccacttccaa gtcccctctc cctctctcta tttcagtctc tctgctttaa
      661 tgtatagtcg gtagcagtga tgtgtttgtc cttccacatg actatgtgat ttggcctgta
      721 atgtggactt tggttcccct gaaacctggg agtgcatacc tgtgagctcc atgacaggct
      781 aaaagtgctg cacaagcact aaatagagga atggtgacgg ttaaggaagc acgatggccc
      841 gcctcttcca tctgtcacat ccaggccgta cgatgaggga tgcttgttct cctgcttctg
      901 tcagtaatct ggcctattgg actttaggcc agagagcaga gacaaagatg ggggaatggg
      961 tcagccaatg gtgtgtgtgt gtgcttgtgt gtgtttgtac gtcacgcagt gggactgaga
     1021 gtttgacaca ggagtattgc agggcagcag agacggaaac aagttctgat ccttgatgcc
     1081 ctgctcggct tcttgtatga cccaatgttg ctccacaaac ctcctctccc aacctcaccc
     1141 tcttaccctt cctcaccctt cttccctcct tctcactcgt cctttatcct cctctccctt
     1201 catctctgtt cttccgtcct cacccctcct caccctttct cgcccctcct tcctgtagtc
     1261 tgtgtttgtg ttgggaagat taagctgctc tgacaaagct gtgtttacaa atatgcaggc
     1321 agagaaacag gccaagcgac gctgaatagg aatagaatac aaattgcttt tttatctctc
     1381 ttagggccag ggcgagagaa aatgtgtttg cgattgttgc tcctccgcct gtgaaggata
     1441 aagcctcagt actgggtttg ggctccctgt tagcattcat tctatcctct cctttctctg
     1501 tctctctctc tctgtctctc tgtcctccca ctccctttct tccccccatg ctctttctcc
     1561 acgttggctg ctccacactc tctgtctctt tctccccggc gggaattttt ttcttgctgt
     1621 ttccttggta atcatatcta ctttcctctc ttctcttctt tcctccccaa ctctctcatg
     1681 ctcctctgtc ctctatacgc gcgctagtcc ctctcccatc accctgttcc ctcaataatt
     1741 ttcttcattc ctctccccct tcatgtttcc acacaagcta aacgtctctc tgtgttgctc
     1801 actctctctc tccgattcca tccatccatc ctttttttct ctcctccgct cagtaggact
     1861 tgtcatgatt tacagtctct ctcagctctt ccttgctcac accggagata ttggaaattt
     1921 ggagtgccta tagatcacgg cagggggaga agccatgagc ctccgctaat cagccctaac
     1981 agaactgtgg agcattacaa atctgggctc tctcttacaa ttaggcagca tgtctgcctg
     2041 gcataaccca cggcagggag gccaagcctg tgatgtcatc ttctccgccg tgtgtgtgtg
     2101 tgtgtgtgtg tgtgtgtttt aaggggaaac aatgggt
//
LOCUS       KCVB01000191            1278 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8784pri ultra-conserved element locus
            uce-1143, sequence.
ACCESSION   KCVB01000191
VERSION     KCVB01000191.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8784pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1278
                     /note="ultra-conserved element locus uce-1143"
BASE COUNT          291 a          402 c          360 g          225 t
ORIGIN      
        1 acttttcggc tccgactgta gcagagcata aaagtaagcc aatgaatggt acatctgtgc
       61 ttcaccagca gcatcacaca gtggagaatt tcacgtctgg gaataaagtg tacaacgcgg
      121 ctccggtgcg ccccgtgaat tgcaaccgga ccgttcctgt ggatttttgt gcccctcaaa
      181 gagacgcagt ttatagtgaa gagggttgtt gcaccaaatc cgaagtggct ctgccgtgct
      241 acacgggcgc ttccgagagg cacaggagat actctctgga ggtgcagggc cacaggtaca
      301 gcaccggctc caccttcgat ggcgtgcctc tcaacaaacc ggtggctctg cccggcaaca
      361 ggtgcaacag tgtctgcatt accagcagcc acgacgggag gtataacgcc acgagtcccc
      421 ggtccagcct cgcgtcctcc ctgtcctctc aggagcagag caagcatgcc agcccgaggt
      481 caagcatcag cagcccgcgt actagtttgg tggtaccggg tcaggacagg tacacaagcc
      541 ccaggtctag tttagttcag tgtgagggaa acagtgttct cagtcccagg tccagctatg
      601 caagcaccgc cagcgacacg agtaagcagt ccagcccccg ggcaagcctc aacagctgtg
      661 actgttgcag taagcccagc agcaaccgca ccagcggcat cagcatgggc tacgaccaga
      721 ggcacaccag ccccagatcg agcacggcga gccagtactc cttcaccacc agcccgagat
      781 ccagttactc tgactcgagg tacgggcccg gggtcaacca tgatctcgat ggggcgattc
      841 tacacgcagc gccgctggcg agccctcggt cgagcatctg cagccaggac gggggcgcaa
      901 gaccagggac caccgcaaac tgcgtggtga gtccccggtc cagcatctcc agtcacagct
      961 ccaggagttc ccgcagctcc agggggtcta tgagcatcta cccggaccta cagctgccct
     1021 cacccaggtc atcgatgctg ggcagcagtt tacacgaaga cacgctgctg caggagttcg
     1081 gagactccaa cgggatccaa aatcgcatcc atctccaggg actctcggcc gtgccagagc
     1141 cccagcagca gtccgcccag acgggaggga ctgcggagat gaccgcgggg tcgccttcat
     1201 cctttagtta tgggatgtcg tcaaaaacag cttcctcagg ccagaggttt aagctgcctt
     1261 accaggtgac ccccagcc
//
LOCUS       KCVB01000192            1410 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8828pri ultra-conserved element locus
            uce-1282, sequence.
ACCESSION   KCVB01000192
VERSION     KCVB01000192.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8828pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1410
                     /note="ultra-conserved element locus uce-1282"
BASE COUNT          357 a          326 c          301 g          426 t
ORIGIN      
        1 gttatacagt atatctcaat atttatgctt ttgcaattaa agaaaaaaca ctgccagcaa
       61 tagatattaa tattcacctt ttgattctaa agtatgagga tgaagcaaaa agttaaattc
      121 ttttggcaat gactccaatc tgatttgatt tccaattgat gggattcagt agtgaagtgg
      181 aagcatttca ttaaaatagg ttccaggggc aagccagtta ttttccaagt gtctctggaa
      241 gcatatcttg aagttagtct gcattattac agtggcagta gctaataagc acactgtaat
      301 cataagatgc taatattcaa gattaacatc cttagtagat cgtggaggca agttgagcct
      361 cagcactctg acgagccaaa ctgtgacagt aaacactggt cgtaaagata aagagaatac
      421 ttgagtcttc atgttattca gactcccgtc tttatgcctc ctctgcctca gtgtttttcg
      481 tcacaacctt ccccctgcct gtctctctat ccttctctct ctctgacatt cactcagtcc
      541 ctgcagtctt attgaccttg gttccttctc atttgcatcc accatctttc attcttcgga
      601 atatggcgtt gggggacttt tccatcatta agtgaattta cagcgatttc cttatgtgtt
      661 ttctccaagg gattgggtta tgagatgcac attaaatatc cattaatacc tcattatccc
      721 actgataaga ttaccatgat aatgaggcag aaattctaat tgtttcactt cttgttgtgc
      781 cactcattta atcatccacc tgaaagacaa gtactctgcg cccctacctt gaagactgta
      841 aacacggaca gcgggataga gctctcccac agtcagtgag agccctgccc ccgccaacat
      901 cagtacagac tgttgcctgg gtgctggcat gcatgcgtgc tcatggcatg cgtgtgccat
      961 gaagtgggag tgtgacatac cagggggtgc ggctaccaca ggtcgacttt ttggtcctga
     1021 gccttggcca ctgactcctg ctcacacctg ctagctcttt tccccctcct acgcattctt
     1081 ccgccttgtt aagccacggt ttcccaaatg gagatggagg gacactgtgc tgaggagttt
     1141 gtgggagggt gggcgaaaca gtaatttggt tgtgagtaat gctaggctta atcaacagct
     1201 caaagtcatg gccctggagg caagggaagg gtgagatatt accattaacc tctgaccttt
     1261 ggcacagtgg tatatgcgcc agtgggtccc agcatgaaat gcatgtgacc tcccactctc
     1321 tctgtctctg tatgcaaagc tcgctgtctc tgaagcccaa ttcacactag attcacagag
     1381 tgtttatgta gtgtttttgc ttatctcagg
//
LOCUS       KCVB01000193             963 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8873pri ultra-conserved element locus
            uce-109, sequence.
ACCESSION   KCVB01000193
VERSION     KCVB01000193.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 963)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8873pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>963
                     /note="ultra-conserved element locus uce-109"
BASE COUNT          257 a          203 c          294 g          209 t
ORIGIN      
        1 gtttttgatg tctagacaaa aactcaatgt gtagttaata gcttttcctc tattttccct
       61 tctgtcctcc aaagaagtga atatgaaaca aacatgcaag tcaggatgtc tttttgtggt
      121 atctgtgtca ctctcctatc tctacctccc tttccctatc gctaaccccg cctctctcct
      181 cctcattgct ctgaagtgac agtatatgtg tgtgcacttc tgagtgtgtg tgtgtgtgtg
      241 tgtgcgcgtg cacaacggca gctgactgtg aaggtcacgc tgcatcacac aacttcctca
      301 gggcacagaa taaatcaact ccttctccct ccttcccctc cgctctgaaa gagaaagaga
      361 gagtgtatgt atgtgtatgt gtgtgtgtga aaaggggaga gtgagaacac aaaagagagg
      421 gagagagagt gagggaaaga gatagctagg gagagagggg aggggagtgg aggaagcaga
      481 agaaaggcag agcaaaggat ggcaagaaag acgagagagg aaaaaaaaca aagccagaga
      541 aaatgtgatt tccagagcgt gagagactct aagaaaactc tgagtgacaa tgattgacgg
      601 ccgggctata tcagtccggt attggcacgc atacccctca ctcccaccct ttcggacgac
      661 ggcccccgct tgccgttcgc ttccgtctga tgcccaaatt aaccttctgg gcgaagtgaa
      721 ggaggcccaa agcagcctaa tcagaggctg attacggcgg cggcggctgg ctaaagggtc
      781 agggtaattg tgccatcaga ggcctgattg ttttcaatca gacgggcggc cgagtgacag
      841 ctgggggatt ggggccctga tggagccgcg gtgggcctaa tcagagaaga gaaggggaga
      901 aagacggaga gggagagaga tcggagtggg agggcgggag gggggggagg ctgattggtg
      961 caa
//
LOCUS       KCVB01000194            1710 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8918pri ultra-conserved element locus
            uce-661, sequence.
ACCESSION   KCVB01000194
VERSION     KCVB01000194.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8918pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1710
                     /note="ultra-conserved element locus uce-661"
BASE COUNT          449 a          363 c          346 g          552 t
ORIGIN      
        1 gctcagctca acaggatttg tgtctgtgta tgttagagta tgtctcttag cttgaatgcg
       61 gatataaccc tttcattgca caagctgctg atttttcatt tttgccgata ggctgatcct
      121 gtgcatccgt agctttttgt ggaagacatt gaaagttttt gtttaggaag cttccatatt
      181 tatttttttt gccttaaata ttttatattt gttaagagtt aatgcagata acctctttct
      241 agacctcttt ctgtggatca accgtatttg aaaattaagc tcatgagagc aggtcttgtc
      301 aatagtgtat ggtcagattg agaatatatc tcaggtcaaa actacgtttg tcccatgtgc
      361 ttcttgaatt attgctttgc atttatctat gcaaatccag gataagatcc tatctgcctg
      421 cagcgacata tccccctgga tttggtacat gtgagttagg caggttatgg ttgacagtta
      481 aaaaggaatt gcaacaacac atatgtgaag attattgctg tagtcattaa ttgttgcttg
      541 aaaagggatt aggattacca gtcctataga acactctaac acctgtcctt cctcctatct
      601 aacaatgtga cttcagtttt ccttatttca gtatcagttc aagttggcaa aataaaaaaa
      661 ggctgattta ttcaaatgaa tcatcaacgc tggaaatgat tcaatatgtg ccacattcag
      721 atgggtgata tattattccc ttggggagag gcaaagatgg agctgtggag ctgtttcatt
      781 tgtgctgtct ttatcagtgt gtcaggcaga gaggctgagc ttgttctcaa tgaatggacg
      841 agcccttgaa ccataaacct gtacctcctc tgataacagt ttttgcattg tgatataaag
      901 tgccattctc tgtgacgtcc tctctgacag cgctaaatat atcaacatac acaaaatgat
      961 ctgtcagatg aattatttta tttcaaccag ttttccaaaa ctaaggattt tcagtgctgc
     1021 atgcgcagtg tgtatttttg aggacacatt gtggattttg ttggggattc aaagcaaagc
     1081 gcagtattgt ggttgcattt caccaggctt tgctattcat gctcttaatg tttggccctt
     1141 aggtgatcat ttttactgta ctttttgtct accatatttc gtttattgct atttctgctg
     1201 ctgggacctt tagactgctc tgacgtctga gaatgccaca tcttctaagg tgataagaat
     1261 gctccctgaa ttactcagag cccgtgtgat gacatggccc tgattgtcag acgggtgggc
     1321 tggtgacact ggaacacaga cggtgcttgt ctgtttgaaa tattctgttt caagcttcca
     1381 agccctgggc agtcctctct cccagtctcc caacacccag catcgcccac tgaaacaaga
     1441 ctcttccata ctggaaaatc tggccctgtc aactggcagt cttgactagg ttcagcccac
     1501 acgccaagca ccccccacca ccacttccac cccttaatag gcactagagt ttgccacccc
     1561 atgtagatgc cttttggtcg ccatccatga atggcgtcaa tagcatcttc ctacacaaca
     1621 gtgactgaac ggagggcaca agaaaatgtg ggaataattt acactttctc actgtactac
     1681 catcagaaac cataattaat catgatagaa
//
LOCUS       KCVB01000195            1567 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 8964pri ultra-conserved element locus
            uce-624, sequence.
ACCESSION   KCVB01000195
VERSION     KCVB01000195.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="8964pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1567
                     /note="ultra-conserved element locus uce-624"
BASE COUNT          502 a          276 c          350 g          439 t
ORIGIN      
        1 atgacatgag gagctgttgg acgacaggtc ttcaagctcc tccactgtct gagtttaatg
       61 acagaggagg agagcaactc tgaagttaag ctctcccggg gagaggttta aaaagacgac
      121 acgtgtgtcc gtgtgtggag gggaagcagg tggggggagg gaggaggttc tgaccagttg
      181 gcaaaggtaa aaaaaaactg aggctgatca gtgattacag catcgatgtg atggaaacca
      241 cctagcacat ttatatcaat cagataagat gtatgatcaa gacaatgtcc attttaatct
      301 tcacaaaata aaaaaaacac atctaaattt ccatacgcta acatttagtg tggtaaaaaa
      361 tgagggcagc gccttcctgt gtttttttct ccgctgactt tttaatcaaa taatttacaa
      421 actctataaa taaatgcaga gcgaggcgag ggaagaaaaa gtataaagga ggagaaaaaa
      481 tggacgtcga gttcaaaaca gctcatcaaa aaacaaacat ctctatatat ctggcatggc
      541 acccagctac tgcttcatta tattaatttg tgagaggtgc agtgaaaatt acactgtatg
      601 aaaaatggct ggaaaattta aatgatacaa actcatctta ttagcggtga aacattaaaa
      661 aacaaacagg cccctccgtg tcccgctaat ttgtcattgg atgaaaggcg tttgatggat
      721 gcatcttacc ttattcataa gcgaggataa aagagatgta ttccctggta cagggtgtcc
      781 gtttgattca ttactggaac aaggaaaatg aacaatgttt agtcaagatt tgcatgagaa
      841 cagtaaatat tgctatttat cacacaaggt gtgcctgtat aatgaagaag aaaaatgctg
      901 tcttcttaag aaataagatc tactgtgagt tggagggaaa attggagttg aaggtttata
      961 taaacatcct tctctcacaa aaatggagaa aaatgtagtg gattttataa ttgtatgcac
     1021 atgtttataa atgagatatt tttgataaat aattgtgaat ataaccctgg ctgccaaaca
     1081 tgtagaaatc gtttatcaat cattgaaacc ttatttcctt cttgatttat tttgtgaata
     1141 tattcagtga attaaccaca agattcaaca ttttccatac agtcgtgacc ctttacagta
     1201 tttaccacgc agctgtttac ctgtggtctt tcttgctcag gtccaggctg tgctcctgtg
     1261 tggagtcttg gctggcccca gagtttgcat ccactggctc ctgcttcact tgaaccagat
     1321 tgaactggct ggctgaagtc ggctgcccgt tccctgagga tcaagccaga gaggcagagg
     1381 agagaagaga caacaaagtt attgttggtc agcattatta ccctatgacg agatccaatc
     1441 tgactcctgt cctggcccga aaagaatact ccgagtgata attattgcga aattatgtca
     1501 ggccgtcaag ccttttaaat ggtgattgag aggcgggaga tatgagggaa aaagactcct
     1561 ttttctg
//
LOCUS       KCVB01000196            1672 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9007pri ultra-conserved element locus
            uce-1183, sequence.
ACCESSION   KCVB01000196
VERSION     KCVB01000196.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9007pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1672
                     /note="ultra-conserved element locus uce-1183"
BASE COUNT          490 a          351 c          342 g          489 t
ORIGIN      
        1 ttttaaacca gtttctaaat ccatgctgta ataaaataag tgttagacct gcaaaagtac
       61 agtgataaag ttcaagagaa aaataagtga tttactctca cgaaaaatgt gcatatactg
      121 caaacactaa tataataaac ccaatctaca attaggcaga tgtggtcagc tgttggttaa
      181 aaatatagct gctattacaa agtaaatatt tgacttgtgt gtgacatgac acaactaaaa
      241 tacaaagctg tagtcatttt tctttacact cgaggtaaga ggttttgcca cgacattggc
      301 atattttgtg tgagtacatt atgcagtcta tcagtatttt ggttgtacag tgtctgtaca
      361 aatatcgtca aatattttat tgtctgtaaa tgtcttttcc acttttcatc aaacaaaagc
      421 tggatggaag cagttaatga gccaaaagcc tgtttgaata agaagctgct gtaacaatct
      481 ctgtcccaat tagcacgctt tggtttttaa atattccttt tgtgcaaagc ctaaacacac
      541 atgaagactc acaaaacggt cacgttgaaa atttaatagg cttccacagc gatttccttc
      601 atttatttaa aatgtttttt tccctctctt tttaaaaagc tagaaatctg agaggcatgg
      661 caaggcattc tgctgtgagg ggagcagcat tcacaaatac tagacctaat tcctttggca
      721 gcagctcaaa ctgcaacaga cagacacata atcagctggg gagtgttagc gcctcagccc
      781 actagcaaac acaggaccga gcccttgttg tgcatcgctg cttttcagaa taaatatcac
      841 gattgaaaat acagtgggag gaaagaggta ttcatgtgta tatagctctt tgttcagtca
      901 aagatacaga aggagagacg aacactgcct ttcttagcta ctttaccgct cattctttct
      961 caatcttctc tctccttcat ttttctctgt ctctcaaccc cttccttcct tcctccctct
     1021 ctttctctca tcctccactg taccctgtgc gttttggtat tcacacagat gtttggattt
     1081 gagtggcagt tcctcctgag actgtgcaca agcggctgac ttgtctcatc tgcattttgc
     1141 atcccagtaa aaaaaaaagg gaacagagag cagcggaaag catcagcagg cagacaggct
     1201 ctgtgcgagt gtgtgtgtgt gtgtgtgtga atgagggaga gagaaagtgt gtgtgcacgt
     1261 tgcctgttgt ttggcttcgg cagcttaact ggccccagcg ctgttgctgt gtgatgttta
     1321 aaggacgttt caaggtcatg ctttcgacag gcctcaccgc ctctgacata ttaaacattt
     1381 atatttttct gtagccagat gaattataga ttgaacatgc aactgaacat gctacaaaat
     1441 agcaccattg tggttggtaa tagacacatt agggtctaca aacacagcat tgcacacgac
     1501 agcctctagc ctgccttatc tcatcaatag caacaccgac acagggacaa aaaaagctga
     1561 tcttgctcta tgcgatatac ccagacactg gagaatccca gagcagcagg agaggcgggt
     1621 atgaaaggtc catctgcaag tcagttgtgg tcagtgactc aaagatcagg gt
//
LOCUS       KCVB01000197            1004 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9052pri ultra-conserved element locus
            uce-560, sequence.
ACCESSION   KCVB01000197
VERSION     KCVB01000197.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9052pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1004
                     /note="ultra-conserved element locus uce-560"
BASE COUNT          288 a          180 c          268 g          268 t
ORIGIN      
        1 gtaaaatggc agatagaccg tgcaaattgg gtgccgacat catgaggcaa tgttattacc
       61 aaattagctt gtgctttttt cccgcccatc ctgatcacaa ccttgggagg aatggcagaa
      121 gaaaagacac tattgtgtgg cgttcacaga cattattctt tgaacaaata cttgagatta
      181 tacaggaggc actgatgctg cctttaccta agatgtattt gcttttaatt catacatttg
      241 attgtgacat tttaaactat ttctctattg agacccaggg cattacggcc caaatgacag
      301 tgctgagtaa atagaaaatg ggagtggatg gggtcttgat aagagaatac agtgtttgtt
      361 caagttgagt ctgcggccat tgttggctaa ggaatgtctt ttgttctcag gtggagcttg
      421 ttctctatct aatgtttagt catggcctta caacttaata cttctaacat ttggtcgaat
      481 aatttagtcc aaaaagagat tttcagagga gaatctttta gctaaacttg gaaaagagaa
      541 tgaggacaat aacctaattc agtgcagagg acaattatca tgtaaaaaag caaccgagtg
      601 acacatcgca ggccgaggta gcatcactct tttctgaaga agacaaactg gtagattgaa
      661 ggtctcttga atccgggctg tcgctggggg taggggagct cttgtaattt gcggaactgc
      721 atccatggag cgggcataag ggaataagtt ggaaggggaa gaagacagag aacaaacctg
      781 gcaggcacgt tgaggccatg ccagtcctgc ctgcgccacg cggtgtggca ggggggcagt
      841 gccaggaaat gtggcaagag gaggaaagat caaacaacaa gggctgcaga tctccaccaa
      901 tctgctctgt attaatgtca gagccaagga gcagctgaac cagacgtgat ccacaatgtg
      961 tgacagcgat gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgt
//
LOCUS       KCVB01000198            1418 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9093pri ultra-conserved element locus
            uce-525, sequence.
ACCESSION   KCVB01000198
VERSION     KCVB01000198.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9093pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1418
                     /note="ultra-conserved element locus uce-525"
BASE COUNT          426 a          274 c          253 g          465 t
ORIGIN      
        1 agctacaaac taataccagt taagcccaag caaaactgaa ttattaaatc tgtcagccaa
       61 tatcctatta cggcctactt atgaatagtt gattttattt tgtataattg acaaggctta
      121 tcgtacagtg acgcttttcg tggcagcttg tgttttgaga tgcagcgata tgtgtaggcc
      181 tgtggtacaa aaggtcaact cccgtcaggt tcatttaatg tggttggttg gtttccttac
      241 aggtgggatc ttatgacctg tcataagtgt gtcacagagc ggtggaatat caggcagcat
      301 gagggtataa catgctcata cgctctctgg caaaggttga atgcataaaa gaccgtttta
      361 tcatcatcta caaaagcaga gcttggatgt taaaaatata tataagttta ttcttgaatt
      421 gtagactcac aaatttactt ttcgcagaaa atactttaac tgtaaaggta aaaaaataaa
      481 gattcaagtt aataagtaca cacaaatatg tagccctatt ttcttcccac cctctctcat
      541 atgcaaatcc ctctcctgca cacacacgct cacacataca cacacttaag acattaagtc
      601 ctcaacctgc accgggcttg tgagggatat tggcatgtac acgagattga tgaaaattac
      661 ttctagagca tttatttctt ctaataccac tgaaagaaga gtgtgcactc aggagatatt
      721 tgatctgaat tagggaaact gatgaagcaa ttgattaatt ggaatttaag ccatcagtca
      781 ccggagccat cagcagcatc atttacattc caaacaatca gctgatggcc caataggaca
      841 ctgagtatta atacgcagga gtcaatgcga tactgccacg tcgccaccaa cccttattga
      901 caagcacaat gacactttta atccccactt gatcaaattg catatatttt gggatttcca
      961 caccaattca atatatctta tgaattttta atccggtggg aggagcaggg gggttaagca
     1021 gtagagattt atcttttcct ttccttacgg tgaaacacac tggtgacagg aggtgaagac
     1081 gagatccatt attaattatt aattgttttc cttcatcttt tgtttcattg ttgccagata
     1141 tattgtgaca tgtttttatt catatgttgt atgccatgca cacaatgcct tgtggttttt
     1201 aaatgcccca cagctctgct agatgaactc aagctacact tttatcaact ccactcaaac
     1261 actccctgtt tatttgaatt gattttaaac tggatgttat tattcatata aagcaataat
     1321 gttttcttac aactgttaat tagattgtgt tgatcatcaa ttttggctat tttgttcaac
     1381 tctttatagt ctgtattgtt tttaactatc tattttga
//
LOCUS       KCVB01000199            1705 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9136pri ultra-conserved element locus
            uce-315, sequence.
ACCESSION   KCVB01000199
VERSION     KCVB01000199.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9136pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1705
                     /note="ultra-conserved element locus uce-315"
BASE COUNT          512 a          387 c          360 g          446 t
ORIGIN      
        1 gttttatccc cctcctgctc ttcagccaga tgttgtggaa gtagtcttct gagtgcaatg
       61 ccgagaggca agtcaggcag atatgcatct tgggaaggct tcaacagctt tctccatggc
      121 aggcaatagc aagttgttat ttgaatgcat attgagttat ttggatgtct agagtctttg
      181 aatggtttta tattttcagc aaagtctcta ttgactttat tattttactg ttttaatagt
      241 ttgaatttac ttcctgaatt ctacatttgt tagataatta atataaaaaa caaactgtaa
      301 caaacaaccc agcaagtaat aaaaaatgca gtttacacag gtttgtcaat ggacactcca
      361 cttcatgttc ttaagaaatg aaaaatatat aaaaacagct acaatactgc aactgttaac
      421 tgattgtcaa cacaataata ttttaacact ggctgtttct tgttacccag agtgaacgtt
      481 ttgttgccat gacaaagtac aggccaaagg gctagctgtc aacaaaagag aggctaaaga
      541 ggagaaaaaa gagctgattt caaacagccc agatcaaaaa atttgtcaca acaacaaagc
      601 tttggtctaa accaacacgc aggggcccct gtagacttgg agatttgaca aagcccagcc
      661 ctcatttttg gaggcccagc tgtaaaaaat gccatttaat gaacctgtct gctgaagctg
      721 tcccctgcct gtcagaggtg tcagccagtg acaagcactg gggaccgccg cctccagtca
      781 cagacaggcc tgccagctgg agacttcggc cccaatctaa tcacgccacc aggaagggac
      841 cggcgctctg ctcttggtct gtcacgctca gggctggggg agaagcgagg ggacgggtgg
      901 aggggtctgg tatggccgac aagcacagcc ttttgtttcc acacaggttc ctccacgcag
      961 gctcattagg agccacaggc gcatgcgctg ctctgcaggg agatgtatct ctacagcata
     1021 atagccataa tgcaatttaa cgtctccttc ctctctctcc tttattccct tcttctttcc
     1081 cttatttgac agagggcagg ccgcattctg cctgtagaac atcacaggtg gaaaaccgta
     1141 aaatactgtg acagttgaga tgaggaaagg cacaggtgac accagtgttt aggtgaaact
     1201 catatataat aatgtgcaga tgctcaaatg gaacacatca tacttaaaag caataggaag
     1261 acacttcaag tacggcaata gattataatc atatctgact tcccaatatc tttaacccca
     1321 cattgtctga gacactatga cagacgttat taataaatct aacgaagcat ttttttttat
     1381 aaaacctgat gcaattttct cccatatgga acaacacatc cccctccact gcattcacgc
     1441 caaacaggaa aataatataa ttcagtgaga cctccaagcc agcatgactg atatatttat
     1501 atgagactgt ggagcactct gacaacctgg tatcacagca cctccactgc attcttctaa
     1561 gatttgtcta ataggcttcc accataaagc cccacaactg catggtccca ggcggccaga
     1621 gaacagcgaa gtggaagaat aaggggagga tacgagaggc agtttcattt tgagtgatat
     1681 gactactccc ccaggagcga gtggg
//
LOCUS       KCVB01000200             885 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9173pri ultra-conserved element locus
            uce-350, sequence.
ACCESSION   KCVB01000200
VERSION     KCVB01000200.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9173pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>885
                     /note="ultra-conserved element locus uce-350"
BASE COUNT          222 a          184 c          234 g          245 t
ORIGIN      
        1 gtagccctga gactgacgct ccaggtgttt gctcttccct gcctcctctc agtacacaca
       61 ctcatccctc tggatccacc ccgctctctc tctcctttag aacacagctc cttttatctg
      121 agcggggagc tcagtaagag tttgtgaaga ttctgatctg aatacacccc tctaagtgta
      181 tgtgtgtgcg ccgggcctac ggcatgtctg aggtgtatgt gtgtgtgtat gtctctctat
      241 gcgtgtgtgt gtgcattagt gcaaatgtta tggcaacagt gcttgctagt ggtgaccact
      301 ggtactgcgg agcagagcac aagcagcttt ttgtctcatg aagttgcatg tctttctggt
      361 gaaatgagat ggttataagt gccttacata ttcacaccgg atgtttccct gggctgtgag
      421 attatcagaa tagaacgcac tgaccttagt aattcagagt cattcaaatg cataaagagt
      481 agttgtcctt cttcaaccct tcatatacat tagcaagcac cagcgagaga ggcctgcaag
      541 gcacagcaag ctggctttat tgtaattttg cagctgagta cctacagtca ggaggagagc
      601 gaaggagtgc cgtgtgcgtt tgtggcagtc tgttggaaaa taacgacgta taaaagctga
      661 tggaaaatga aagcactctg ggcgggacga gattatgcac aagtgattaa taagagcagt
      721 aataaacaaa ggcccaggag atgtttgagg tgcttaataa tgcattatgt tcataaaaga
      781 gctgggcctc ttcaactgat ccctgtggag taggagaccc cagagggacc aggagagagt
      841 gtgctcttaa aagacagcta ctgtgtgtgt gtgtgtgtgt gtgtg
//
LOCUS       KCVB01000201            1659 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9282pri ultra-conserved element locus
            uce-857, sequence.
ACCESSION   KCVB01000201
VERSION     KCVB01000201.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9282pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1659
                     /note="ultra-conserved element locus uce-857"
BASE COUNT          392 a          416 c          349 g          502 t
ORIGIN      
        1 gcacgtttcc aaagccatca atactgtgga gtgattggct gtgaactact ctaatttaga
       61 tccagatcgt ccgtgtggtt aatcctaatt tgggaaactt ctgtgattaa aaaatgtgga
      121 tacagagcag attattttat ctttgcaggg cagataaact tcactttttt aatctcaaat
      181 gatgccactt tatgttgctg gcaaaattca gtgtgagaca gcagtcagag atttggtctc
      241 tgggtaaaaa tacctgttgc ggctccggag tgatgttgaa gctgcccatc cctgccacac
      301 aagtggctct ctgataagtg attacaattg gggagggtgg ggggtaaaac aagggccttg
      361 tcagcacaga ccctccagat ttgaggtcaa tttcgctccc tgattgcacg tattttcgag
      421 tgtgtgtctg agggaggagg catggacaga gtcagagttt actgttgcct cattgaagcc
      481 atcatcatca catgacgtgc agaggctgaa gcctgcgtct gtcagtttca tccttttgtt
      541 tttccctcca acctgcctgt ttttcattcc tccctgcctc cccctctttc cctccttttc
      601 ttctcctcat ccctatctct gctcctcttc caccccttgg ccctggccct ctgcctgctc
      661 tttcgctcac cccccatgtt cgcaaatcac ttcttcccgt gtcagcaggt aaattgtttt
      721 gtcctcccat tccctgcttt cattaccttg tgtaaattaa gcgatatgtc gtccaccggt
      781 gcgcgccaga gcactcactg cgccaacgcc aaaatcatga gatttggcat aataaatgaa
      841 gacaaactgg cagtagcagt gctcctctcc gcgcgggctc gccttcatta ttcgcttaac
      901 ttgccggaga aaatgactta accctgacct gtgtatttcg tcccatattc actccagata
      961 aaagcctttt tacagcgcac aacaatttat attccagcaa agaggctgct tttctctgtc
     1021 agttttccaa tctcagctcc tgaacacacg tcattaaact tgtcttgcct tcctgtattt
     1081 gtccatcagt ccttcttttt acataaaatc tggcagcatt attctgggat ttgatggcac
     1141 ccactcattt tttccccttg gaatgtttga ttggaagaaa tatagccggg ctggggctgt
     1201 ctcctctcct ccgtggaatc atgcacttga tgaaatagtg ttcccccagc aagaataata
     1261 aaaacagact tgtgcattac agggggctct caatctgcca gctgggctga gctgagcgaa
     1321 aaaagccacc ctgctcctga cagattttta tggttatccc agcagataat ggaaaccatg
     1381 ggtgaccagg ccagggaagg ccacagtgct ggagccgagc gttggctgca gggctggaca
     1441 gtaacatgaa gtgtggtatg aacattaaag ggtctcaggc tgtacccagc tcacaatatg
     1501 tcagttgaat tgggattttg cttttaaata taaaggttgg actattcgtt cctttttttt
     1561 ccagttaaac tccaaactaa actcctacat gagcctcatg ttcaatgtta agactgactt
     1621 gtgcctttct tcgtcatcag ttatttgaaa cagacttca
//
LOCUS       KCVB01000202            1405 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9327pri ultra-conserved element locus
            uce-1063, sequence.
ACCESSION   KCVB01000202
VERSION     KCVB01000202.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9327pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1405
                     /note="ultra-conserved element locus uce-1063"
BASE COUNT          460 a          281 c          320 g          344 t
ORIGIN      
        1 gagttcactt tctacttctt tcataaatca aaaggccatg gtatataaag acggcaacga
       61 gacgactaaa aatcaagagt gcagaggtgt catcactgag tccagttcca gaagagaagc
      121 tatggatggg tggaggacga gcgcaggaaa cactgtgcca gtgtagaact gggatgggct
      181 aaacctctaa gaaattgaaa tagcttttgg cctttgattg aattgtgcta tgtgagctcg
      241 ctacactgta taagccctct ctgtgtctct gtctgtctaa ctctctttca gctccacatc
      301 aaaaaggcca tctgcagtaa aaaggccagc caacacacca tgcgctccca tctcatccgc
      361 aagtttcctc tgcccagcat ccagacacgg cactgcccac cgtctcatca attttactct
      421 ttgatttaat cacccagtga aactaaagtg gctttacaaa gttttatttt gcaataaagt
      481 ttaaaaaaca gaaacctttg ttatcatcga atatatataa aaaaaagaat attattaaaa
      541 aacgaggtgc caaacgaata cacaaggatt tattttacag tctcaataat gcaaactgct
      601 ttcaatccca cagagcatta ggatttaaag tgagcatgaa acacaaaccc ttcaagcaga
      661 ttaaatttca aatcaagcag gccatgtaaa tgatgtggtg acagggggcc cagtgcaatt
      721 agcatgccag gctagtgtta aagcaattag gcaaaacaga cggctagagc accagggcta
      781 agcacaggaa ggcaggaagg acgaatattt ccatattgtt cttcaggaca tgagcgcgtg
      841 tgtgtgcgtg cgcgatgtgc ccgcgcgtct acggttttgt ttgaggtcgt ccggcactcg
      901 acttgtttgt ggatttctca gagggaaata gaggttaatg ggaactagac tttgtttact
      961 gacagcctat ccagccattt tggagagtga gggggtgagg aaactaaata acaaatgctg
     1021 cgtttcttgt tacaaaaagg gaaaaaaaaa gaaaaaagga aacagaaaac actgttttat
     1081 gttgcttagg ggaatcacaa aaacatttac tggtaaacaa gcccagagcg gaaacttctg
     1141 ggaaccactt tggggaaaaa agcagacaca tttgggcaaa tctctcaggt ggagccatca
     1201 aaagcagtgt cggtttgtac acggagacgt ggcagaaaag accacctgga gacgagcgat
     1261 gacattcgtt tgagcgtaaa ttactcaaac aagcatgccg gggaatggca tttaatactt
     1321 gatgtttccc agagggaaca gtggtggatg cctctaattc aaacaaggag ttttgatata
     1381 aaaacactgc taaaaaacaa acatg
//
LOCUS       KCVB01000203            1580 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9368pri ultra-conserved element locus
            uce-739, sequence.
ACCESSION   KCVB01000203
VERSION     KCVB01000203.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9368pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1580
                     /note="ultra-conserved element locus uce-739"
BASE COUNT          412 a          377 c          308 g          483 t
ORIGIN      
        1 actctccctg gtcaacttta gtgaattagt tgtgtctcat tctagttagc atggtagatt
       61 aaaaccctaa attaaaataa gcatgacata gacaagtaga acaatggcta tggtgtctgt
      121 aatttcctct ccttctcttt gacttggagc atgctcatgc atgactactg gatggatctg
      181 aactacctgc ctccatatga tctttttaca atgctcttga cttgatggtt gcaatataag
      241 ttgtatggtt tcaaagtagg aagatacaat agcctcaatt tgaaggtaac aataaaatgg
      301 ccagattttg ggagacattt tgtaaaaata acctaatgat tatattgcaa tggcagcaaa
      361 gcctccatta ttgcatgaca ggactgaaaa tgcattcatg cagaagcagt tctccttgca
      421 caatatctca caatccattt attattcaat cgggatgagc taattacatt caaacacaat
      481 taggtcaagt agccctagac cactgctccc actttggctg agacctgcat gcatttacag
      541 catacacaca tcttccttga aaccagcatt gagatagaaa agaatgaaaa aatgccctgc
      601 ccccacccct aaaaatctga ccctcaacta ctttttcctg ccaatgctgt tgtgcacttc
      661 cctctgccgg ccaccggtgg tgatgtggga tggtgttcat gtcctctcca tgggtgcttg
      721 attcttcctc tcctcctgac agccgactac tgaggttgag gtgaagaaga agctgtcggg
      781 ctccctgatg gtgtctgtgg accagctcaa tacctccttt gggcggaaag agcgggccaa
      841 cagtgtcatg agcgtcatta ccaacacact agtggagggt actgccactt taacccagct
      901 atttaacctg ctctcattat tctttaccta gggctgctct cattggttga taagtttgac
      961 atgaatttcc tgcttatctg ctttgcatat ccaacagtac cagatacata caatgcatac
     1021 aattttgtct atcatttgat accattgcat gcggtatgct caagcacgca agtatgtttg
     1081 attttgatgt gtgatccgcg ctatgtcttc agaactggag gagtctcagc gcaagtgtcc
     1141 accatgctgg tataagtttt ctaatacatt cctgatctgg gagtgttccc ccacgtggat
     1201 taagatcaag gagattgtga acctgattgt catggatccc ttcgtggact tagccatcac
     1261 catctgtatc gtcctcaaca ccctcttcat ggccatggag cactacccca tgacccccga
     1321 ctttgaacac atgctgtctg taggcaatct ggtgagtggc aacctttttg ttctgttatt
     1381 tttatctgac tcaccggtta aaaacctcct ctgcttcttc tttgcagctc atagagaggt
     1441 gttatttttg ggaccgtaat tggcagccct tttattgccg attcagagtt gaaaggaaaa
     1501 aaaaaacatt ctcaaccacc ccttccctgc tgttttcttg tatccatgca tttctcattt
     1561 cttcacacat acacatgcac
//
LOCUS       KCVB01000204            1559 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9412pri ultra-conserved element locus
            uce-994, sequence.
ACCESSION   KCVB01000204
VERSION     KCVB01000204.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9412pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1559
                     /note="ultra-conserved element locus uce-994"
BASE COUNT          332 a          420 c          309 g          498 t
ORIGIN      
        1 agaatactcc tgtctgttac atttgtctct cctggtatca ttgtattatc tctgtctctc
       61 tcacagtttg aatttatttt taaaagttag ctatttctct gtacatgttt gcttgcagct
      121 agtctatctg tctctccctg ctttccccac agtgtttatt tgctgtagta cccactgtgt
      181 ttttaaagta agccagcact aagcaaacac tatcgatatc tcctattccc atctctcctt
      241 tcctcattcc ttcaatcctt tacatctctc ctctcatctc tgtcacctct ctccacaaac
      301 atcatttcta ccgcactcct tcacctctcc tgccatctgc ccctccaccc tccccccttt
      361 tcctctcact ctccaacatc gcacctctat cttcgtggcc ccctctttca ctccgctgac
      421 ctgcccttaa ttggtctgct gcagttgcac ctacgggatc cagccagctg tcaaacagat
      481 ccctgcctgt tgaggttaac cttaagtggg cctgagtcgg ggccagggcc cgggttttga
      541 gtagttctca gggtctaggg cctcgggcac aaaaggaggg ccaaaagcat ttctttgaga
      601 gcaccaaatc tcctgtctcc tcttctccct ttccccgggc ctctccttcc tccttcctcc
      661 atccctacct ccctttctcc ctccatccct ccttccctcc cctctgcccc ctccagactc
      721 tctgtctgcg ctgacaccgc ctttaataag ggggagtggg gtgcactgtt tatttgtgtg
      781 gtgcgaggtt aaaaacttcc cccttaattg acattccggt aacactttcc aataatgggg
      841 cgagcaattt ccttgaaatt gatgtgtgtc caccgcaagg aaaaacaccg ctcctagctt
      901 tcactgaccc atggatgatc tacacttgca ctgtggcttt gtctgcgtgt gtatgtgtgt
      961 gcatgcgtgt ttgtgtcagc gtgagaaatt gcaggaatgg tcttttgttg gcaacagcag
     1021 catgtttgtg tatgtgtgtg tatatataag aaggcaagca cgcatcatgc ttgtgtctat
     1081 gctgctaatt cgtgtctgtc aatgtgaatg tgtgcatacg tatgtacctg tgcatgcttt
     1141 gcacatgttt acgtgtctgt aattatcttg ttttcggagc ttttgttagc ctatttactg
     1201 cttgtatgcc atttatttct gtgcccagcg aggcggacat aaataaacaa ttcaaatatt
     1261 ttcattttca tttatcccac acaccaatga ctgacagatg caccacgtca aaatgctaaa
     1321 acaaatccac agtagaaagc ctattttgcc cgcagctcag tcaatgtctg tctgtgagaa
     1381 agtatgcaaa cacaaacatc tctctctact gcagactgca tccttggcct aaacagtgac
     1441 cactggttag gacttgtggt gtgatgacgg ttagtgttaa acatgtgtgt gtatctgtgt
     1501 gtgtgtgtgt ctatgtgagg ggaaagagag agagtgaaag agggagagtg aaagagaca
//
LOCUS       KCVB01000205            1550 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9486pri ultra-conserved element locus
            uce-1219, sequence.
ACCESSION   KCVB01000205
VERSION     KCVB01000205.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9486pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1550
                     /note="ultra-conserved element locus uce-1219"
BASE COUNT          446 a          308 c          365 g          431 t
ORIGIN      
        1 ccatccacgc ggccttctaa tggagaccat taggccagag ctgatgtctg ataaagagaa
       61 cgatatcact tcaaaaaacc caaggaacaa catataatcc aaacaaggtt gctgtacata
      121 gtagaaccgg agatttccgt gcatgttatc catgggcaat gggagggggg gtgggtgggg
      181 gttgtaggga ggtgggtcgg gtgagcgcag cgggggggaa ctggggggtg ggggtgtcaa
      241 ggatctcact cagctgatgc ctgaacagac gagccaccac tgatttcatg agctgcagaa
      301 gaggattttt tgattacaat aagtatcaat aaaaagtcaa tgggactctg agacatcttt
      361 tctctctcaa gagctttcac tttctctttt ttcttgtttt ttttttagca aaaatatcat
      421 gcagtcttta gttccaaaag cccctggtgg gatctcgtgt ttgtttgttt gtttgtttgt
      481 ttgttccttt caggaggaaa aagaaactca aagccttgaa actcttctct cacaattatt
      541 cttaaacatt caagtcatcc tattaatact ttgtacagca gaaaggtcct tcagatttca
      601 catgtgtata tataaatata atatatagaa atatgcatat acatatgttc aacttgtata
      661 tgtgtataca tattttggaa ctgtatcaaa gccaatttaa tgtgctctca aaatatggcc
      721 catgattcct agtgggatgc acatcaattc acatagtaca accagtagat taggttgaaa
      781 aatccaaagg tgatagggaa tatgacccgg gaccacttgt caatggtgct aacatctgac
      841 aggtttggga ttttcaactt gagcttggag gagcgtcgcc gtagccggca gttggcccga
      901 ctccgcatgg cgctccggtc cagcgagtgg tggctgaagc catctcgggg tgccatgggc
      961 ttcctgaatt ggaccccgga gctgtcgaag gacatgacag aattccgaga gtcactgacg
     1021 ctgctcccca cgtcagaagg catcacttcg ttgttcatct ccagcgtggt gaggaggatg
     1081 tttccgtaag catccacctg cgaggagagg gggaagacac agagagtcag ggagagctga
     1141 aatgttgagg agtggcccca aattccctta ataatgaaac ttatattaac ccccctccca
     1201 tccataagct gtcaagctaa agcaaaatgt agggatgata tggaacgttt tttggtgcat
     1261 gcaacagaaa tagttaacaa gagtttcttt atacaaacat ctattagtgc tttttcactt
     1321 gcaaaaaagt cacaagtaat ttaatcagcc ttaatctact gactttctag cgaaggctgg
     1381 cctacagctg atatgatttc taaatacaag taccccaact tttctttgtt cctcgagata
     1441 cagatttctt cgctgtgtaa atgacctgaa ggtgttggtt tgaaacggca cggaaatgga
     1501 gtccttgcaa tgtgaaagaa aaatgaagag aacattaaac cgactataaa
//
LOCUS       KCVB01000206            1355 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9563pri ultra-conserved element locus
            uce-642, sequence.
ACCESSION   KCVB01000206
VERSION     KCVB01000206.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9563pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1355
                     /note="ultra-conserved element locus uce-642"
BASE COUNT          424 a          259 c          264 g          408 t
ORIGIN      
        1 tgttaatgaa aacgaagtgc atttgattat ttgacggacg gtgatgttta atgtgtcgtc
       61 gagaggaggt cacatgttgc agcgttttta cttctaatag atcagatcat ttagaatcag
      121 tgaggaggtc tgacaagcag atctcgccga ttagaatatg agcgcaaatg tagaaacatc
      181 tgcttaacat acgcgtcgag aaaaattggt gacgtcctga cacattgttc tatgaaaaac
      241 tgcctcatta aaatatgtta aaacttcagt atttttatat tcaaatcaag atgaaaagtt
      301 cattaaagag aataaatatt tcattagatg ctaaataaaa caaagggaat ttttggcttc
      361 tctcagcata aaattccttt tgaaagatta attaatacat gtaaattatg caaattatgc
      421 ccatgcaaat attttatgaa gacaattaag ggatccatta attacagtct tttttgcttc
      481 agtgacattc attcatttaa ttttaatttc actttaactg ttttgatgtt taatcttgca
      541 acaaagaact ttatctctgt gcacagggtc gtgtaaactt gccgaacaca tcgagtgggg
      601 aagtggcagg gaaccaggga agatactggg ggaagatatt cacatgtgca ccgttgccag
      661 gcagtactgg cgaggtacag tcccagtagc ttgcctgcct gtgaaaagag ccacgttccc
      721 tcttggaccc gattcaaaac actgtaaaag caattaataa acacagactg tgttaatatc
      781 gcagaattag agaagtgtga caaaagacaa atagaaataa ttagttctct aaaattatgc
      841 cctcattatt tttttccccc tctctctctc ccgctctctc cattcgcctc ccttagtttg
      901 gagtcgagaa tttacgcgca cattcctctc agctttcatg cgagtgatag gcttgttttt
      961 tccccgtctg cttgtgtaat ttatcaacat cattttttgc catttgaggt taattataca
     1021 gcgagcctcg cagagtggag cagaggagac atgggtggca gacaaatgct tctctaattg
     1081 aacaaaaaca caaaatgcag cgtgttattc agcttcctcc ttcactccac tcttcaaggc
     1141 aatgatcaga gtgattgaga gaccgcagac actgcatgct ccatggctgc agcatactct
     1201 tcctaacggg gattagataa aaaaaagcag taaaatgttt aatttctaac tagactgtag
     1261 atataagcag gacaatggct gctgtcactg tgcaagtgca aagttttagc acatttaatt
     1321 tgatacaatt agactacaaa aagagccagc tcttc
//
LOCUS       KCVB01000207            1237 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9605pri ultra-conserved element locus
            uce-1118, sequence.
ACCESSION   KCVB01000207
VERSION     KCVB01000207.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9605pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1237
                     /note="ultra-conserved element locus uce-1118"
BASE COUNT          395 a          245 c          248 g          349 t
ORIGIN      
        1 ttttttaaaa aaaaagtact tacagagtaa tttacttcta ggtatttttt ttgcactaat
       61 ttactttttt tcctgtactt ctcgtttgta cataccagaa actccctcgt aataatttca
      121 ttagttcccc tatctataaa ttatttacac atggttgcag tcttaagtat tttcctagtt
      181 tgaatttcaa acatttagat taaagaatct aatggtggtt tggtgaacca atatttcgaa
      241 acaactttca agtccacaaa cactgtctct ccaaagacct cactgctcgt gaccacttca
      301 ttcacacgtg tttcagctgc agaaaatgtg ccaacgagct ccttgtcacc tgaaactgca
      361 atttgtgaca acattctgcc aagtggcaag aggactgtcg tttattaaag gggtaatata
      421 cacagaatga caccccgttt caaggatgat gttactaatt gtctgctcag agagctgtgt
      481 tgcagtccat tcctttctaa tagcagcaag ccaacagaac aagtgtgagg gcaggcgagg
      541 aagtgaatta gggcggtgat tatgcagtga ttcagacagc agctcattag aacccagcta
      601 agcaagcaat caacgaatcg atgtgacagt ggacccaaaa gccacagaaa tttgtaataa
      661 atggcaaagc gataaattgg attatccaca gccccgtggc caagctcttt ctgttgcaaa
      721 tgacaaatca atccaatcac gccaggaaaa agccaaaaga accaggaaag gggccttgtg
      781 ttgcactggc tggggaggtt atcttactga gatgtgcctt gttaaggttg tggtgtttcc
      841 ggaggtcatt tctgtatgat atttagggct ctaaaatatt catgacacac cattaggtgt
      901 ttatgaccac cttttataat gagagattta ctgatatttg aactcctgcc agaatagtaa
      961 atgtgtcaga ggggtaccat gccacatgcc tgcagattca ccgagaagaa agcagctctg
     1021 caccgagatc gtgatgatgt aaatgagcag ccaaggagca ataataaata aataaaatgc
     1081 agagacagca gcttctcttc ctacctcagg atattcaaag tacaagtgat ggatcgactc
     1141 tatatacttg tcatatcata atcactgaat gtcacaatca tgactgaagc ttgaagaaaa
     1201 accgatatga aactgatgaa caaagataga tagatag
//
LOCUS       KCVB01000208            1381 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9720pri ultra-conserved element locus
            uce-874, sequence.
ACCESSION   KCVB01000208
VERSION     KCVB01000208.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9720pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1381
                     /note="ultra-conserved element locus uce-874"
BASE COUNT          413 a          240 c          316 g          412 t
ORIGIN      
        1 aattgactat ttaggataaa caaataccag gctgtcactc agtctggcag ccatccgacc
       61 acctcagtca cagacagcca gtgatgccag tgagatggtg ggatgccaaa acagtagatg
      121 gatgtcacgg atctttttga ctagagctcc atgctgcagt gacagaccta gagtaactat
      181 ttgagtatcc ttaaactgta catcaaagtg ctacatcaat atagagtttt tgaaaccata
      241 tgttgtcaag tttaattctg tgagggaaat atattctatc ataaagttta gttttaagct
      301 atttttactt gccaacagca gaatgagaca cctagttttc cctttgccaa ttctgtgttc
      361 tcttttctaa agggaaattt gaagggatga tctgcgcata ttcgaagtat aaactcccca
      421 taaacccagt tctgctgtat gtgaggaagt gtctgtttgt gtttgtgtgt tccttctgat
      481 acctaaatcc cattgtgtga agaggaagta gcagggtaga gtagctcggg cccctgacca
      541 agcctgtggg tgggtggcat ggattcataa atcagggagc agtgttaatc tctctcaggt
      601 attggaaagc cagccctcag taaatatgtt gccccaggat agaatggggt ttaaaggatt
      661 tctgtctcca aagagaaatt aggcaggagc ttcagaagtg agctaaatgg aaatgcttgc
      721 tggagcatac agagagagca gggtgctgct gtcagctagt aaacatcgtc tcagagtagc
      781 ctcagtcatt atcgggacag ttggtgccaa cagtcagggt gagcagacgg gtgggctgat
      841 gaagagcatc ttctatgctg atgattagtc acaataggga ggagaaaaat ctgaggactg
      901 ttggattaca ctgttgggag tgagaattat ttgtgctata agtggctaat tacagaaact
      961 tacaatcaca tcccacccac tttgtgctat gggattgcgt ttaaaaaaaa actgacagag
     1021 aaattaactc tatcttattc agattctact aggcttacat gctttccgct tgtactttac
     1081 agtatatgat ttcaaattgt gacatatagt ggatattatt ttaaaaatca cagtgtgaat
     1141 tttgaggcac actgtgcatc gacagtgagt caaatgagtt aatgttcata tgtgtaaata
     1201 tgtttaaatg gcagcattct ataggtgatc gtaaaagccc attagagaac cgttcccttg
     1261 gtgaaactaa caatcttgta gttggtttta taagtaatat aaaactgaac agattgtaag
     1321 aaggatgcat agaaaaagtg tgtttgtttt tttctctaca atattcactc ctgacaatga
     1381 a
//
LOCUS       KCVB01000209            1227 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9763pri ultra-conserved element locus
            uce-1005, sequence.
ACCESSION   KCVB01000209
VERSION     KCVB01000209.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9763pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1227
                     /note="ultra-conserved element locus uce-1005"
BASE COUNT          359 a          247 c          288 g          333 t
ORIGIN      
        1 aggagtgagg tggcaacaga taataacaca aataaaacgc atggcagtta aagcgaatat
       61 accagctgtg ctgtttgaaa tcgctcgtcg tcactcagcc ggttgtgtag gcgtgtgatt
      121 gctggaatgg ggcagagcgg agtgtgataa acttacaggc tcacttatgg aaatgtttgc
      181 atgggctctt gacagatgag cgacacttgt gtttgctaca gtctaaaaga cttccctatc
      241 tgtgtctacc aagaccacag tagcaggtgt ctaatgtgac cattttggtt attcaacaca
      301 tgagtccatt atgaccgctg agccgtgaga agaatggggg tagggggtga gagggggggc
      361 agcctgctcc tttaaatgga atctttaatt tcacaggcct cttgatatcc cgccgcagtt
      421 gcgattgtgt aacagaaaag aggaatcaag gcttctgaaa gcacattaag cagaaagcaa
      481 ttaaccaaca cacagtagtt aatttttgta ttaatacttc aacagctggt gcagattgct
      541 tcctcatttt tatctccgct ccagctgttc ttcctccgac aagaacatta aagaggattt
      601 gtccgtaata aagtggctcc atatatagat acctcactga gaacagaacc cgtagggaac
      661 gtggatttac atatagaaaa agttggggaa acggacatct tttcaatacc ccgctggtct
      721 tcctttgagc gagcacctcc aatgggagct gtttcaaagc ccccttttct tgtctacaga
      781 gtgttggcat tggtgtacat ttcaaagtcc tcttttgaca tcctctacag atgtgctgct
      841 tggaggaaca tttgttatat tgtgaattgg ggactaaagg caaaggccca ggaacatttg
      901 attaatgctc atacatatgg tacactaggc agatcggctt gtgtgggagt atctctgctc
      961 tattctaaaa agccagaagg cagagggata aacaaaaaaa acccgacaac aacaaaaaaa
     1021 acaaattaaa ttactttttt tatacgtctt tgggcaacag ctttccatat ttgaaggata
     1081 atgtggcttc tatttctgac tccgaaaacc aaatataata tgtttttgga tcaggcttgg
     1141 cgagtggggc ctggccatgg ggtcttagct gactgtcaaa acaaacatac caggaacaag
     1201 ttgagacagg ctgccactag tgccaca
//
LOCUS       KCVB01000210            1606 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9806pri ultra-conserved element locus
            uce-1040, sequence.
ACCESSION   KCVB01000210
VERSION     KCVB01000210.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9806pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1606
                     /note="ultra-conserved element locus uce-1040"
BASE COUNT          494 a          359 c          304 g          449 t
ORIGIN      
        1 gcatgaatca acactgtcac ccctgtttta gatgtgtttt taaaataagc ttagttttta
       61 aagctgttta tatacctaag ctataaatac taattccaat aaaatattgc ttaaaaaatc
      121 ttaagaatta tctcaaatca aaaggaacta aaaggaactt cttatacaga aaacatgctc
      181 cagtcttgtc acatagaatc cactttaagt ccagtgtcat gttgctgtag tcgctgggaa
      241 gtcttaagtt tcccctgaaa tttagtgaaa gaacacttag tcccccacat ggtaaaattt
      301 cccaaggtct gataatataa catttgtcct tattcgtacc actatcacaa gacgtcagag
      361 tattgtgaag gatcgtgtaa ggaggaaaaa gcaacaccta tgtgagagga actcgatgta
      421 tggagcatgt tgtgaacagg tgtggaaggg agctggtcag cagacagtgt gagagcagac
      481 agtcaggagg gctagagaga ggagtgagtc atggaggcat ctctacagtg taaagcttgg
      541 tattctccaa cagaggaaac aggcttagtg gttggaggat agatcttttt tttttccttg
      601 ccacagttaa ttttcaaatc tccaacatcc tatggctgtc tgtttttctt cttcttcttt
      661 tctgtttttt ctgcttttat tcttttcttt taaaatgaaa ctggagctgg ggcggtgata
      721 gtgatagatg gctcagcgct ctaacacttt atccaatccg gcgcagattt catccaataa
      781 agaatcactt gtgcactctc aaactaaatg gaagtcaggc agcggagaga aaggggctgc
      841 cgtgtgtttc tgagctgcga aaatcaaatt ttcttaaagc tgccgcagca gtggctggcg
      901 gaaaaggcag caacagccat gactggccag ggccacgtct gcacacacaa tttaatccat
      961 cacagagcgc ctgacaccat atcaatcaac ctttcatcag catgcccccc aaccccccaa
     1021 aaaaaggaaa aaaaacaata tttctattca atcacctctg actgaaccag gcctgcaacc
     1081 cactgaccaa tggggaggac cagaaaactc cagctggttc agaaatcctt acacatgaac
     1141 ataagcagaa agtagtacac gtgcactctt acttctcatg tgtgtgcgcc cctccacaaa
     1201 aatggtggag aaaatattaa taaatccaag tccttcctat tgctgccacg aggcacaaca
     1261 aacatgtgaa aaatatttct acatgtagaa atgaatagat ttaaataaag aaagcataaa
     1321 caaacagcat ggactccata aagtgctgct tgtttaaaat tttaatatca ttaccacaga
     1381 gatattactt atagcatgtt tgaccatgac agcaatgaca taccatcaaa ttttctgtcc
     1441 ttctttaact tttaccgtat gacacttacc caccacccct ccacctaacc ccctcccatc
     1501 actgtccttt gttccgggaa tatagtccct cttaccccct tctgtatccc acaggctgtg
     1561 gctccagtgg cctatgattt ataattcata ggggccgctt ttatat
//
LOCUS       KCVB01000211            1626 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9852pri ultra-conserved element locus
            uce-373, sequence.
ACCESSION   KCVB01000211
VERSION     KCVB01000211.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9852pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1626
                     /note="ultra-conserved element locus uce-373"
BASE COUNT          450 a          351 c          381 g          444 t
ORIGIN      
        1 taaaaagagg ggctatagta tcagtgcttt gcttttagta tacaccaaga aatgagatct
       61 gtactgatgt gtagggattg ttgccagagt agttgcagta gtggtgtcgg tttcataaaa
      121 tatccaacca aggaggcata attgtgagca gatcatttct ctcagcgtat gcagaattaa
      181 cttgtgcatt gaaagccaga cttatggaga agggggagga ggagttggta gggtgagctt
      241 gttggcttaa atcccgactt gctattgata aacttgatga atgtaatttg tgcctgcaac
      301 cccgggttat tgtcccccct ctatacctgc aatgccagaa cgctctgttc ccgcgtgcag
      361 gaaagcacaa tggcccggtc cgcgttggct cccatctgaa tacctgtatc tgcctgtgcc
      421 tctgctgagg agtctgacac acaaagagaa aaataatctt ctgaactaag cttgtctggc
      481 atatccactg ctgagtctat aagagcagag aaaatctcct caaactgatg cactgaaata
      541 aatacgctta atttaccttt gcagaagccg cattggagac cgcaaaggcc ggcacataaa
      601 agaggcatgg atggagttgt ttggagattg agatacaaga cagtgcaggt tagtggagca
      661 tgctaggcat atagcgaaat gcaaattcac tgagaggggg agaggatgca acaaaaacac
      721 gtttgataca ttgagtaaaa tttattccat gcatgcgaat gtgccacccc aacccgagca
      781 catcatttgc tcgacattca gtacatctgt tgtgttaagt gactgtaatt taggtgacat
      841 tggcacaaag cctcttaatg tagccccggt ggaccccctg taagaggggc acgcgggacc
      901 aaacagaggc atctcgcagc tggagccgcg tcaaggatca tttccctctt cacatgatta
      961 attacagttt gatcacttga atctaaatct gattaatttt ggtcacatgt cagggaggca
     1021 tcagtggtct agaatgtata ctgtgctaat ctgtaccagc ctctccttga gaatcatcgt
     1081 atgaggttac aacatgtgaa gctaatgtcg tgcatgtgtg aagtgctgaa acgatcactt
     1141 tgtcatttac atgtgaatct ttggcattgg tgtgtaaatt tccagaattg gcatcatgaa
     1201 agggagacac gctgtttaat gggcaactcg tacaatgagg agatttaagc tatacattta
     1261 cggtctccat tcttcgcata ccgcttgctc tgctaatgtg taggagaaag tagcctgcaa
     1321 gctgcgtgta caacattaac cttctcctgg aaatcgcctg atgaggtgta ttgaaggagc
     1381 tgcactcata ccaactcctc catacttcat gtcattcaga gctgttttct tttactctga
     1441 cactcatgag acctaacaca aaaaaagcct caaacactca cacacatata cattccctca
     1501 ggactgcagg ccggattcct gtcctaagtg gttgttgtgg aacttctgag ctgtcaaagc
     1561 ctcaccataa tccctctcat gggtcagggc acagtgtcag atctcagttt actgtacggc
     1621 tttctg
//
LOCUS       KCVB01000212            1352 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 9997pri ultra-conserved element locus
            uce-463, sequence.
ACCESSION   KCVB01000212
VERSION     KCVB01000212.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="9997pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1352
                     /note="ultra-conserved element locus uce-463"
BASE COUNT          414 a          299 c          275 g          364 t
ORIGIN      
        1 aattttcctt caaaagttgg gatcctttct ttaactttgt gggaccataa caggcccaca
       61 ctcccattat tccatatcta ataatatata cagtaccagt caaaagtttg gacacacttt
      121 ctcattcaag ttaatgggaa ctggcactgt acacaaatgt ctacttgtat acagtatgga
      181 catgctgcta tctagtatta ctctcaatta ttttggcaca taatgagcac tcactcactg
      241 gcataagcat ggtgaacgta cactgactca tagtgacaca cagtccttac tcgttcaccc
      301 ctatcctcct ccgcaacccc atcccaagag agattcagac acacacccgt gtaaaacacc
      361 taaacaagtt atctgtcatt cttattcaac tcgtgttatg aatcttttgc ttttctttta
      421 tttctttttc ttacttctac atctatgtat gtttgtttag atgtaactgt atgtgtgcta
      481 tcacaggaaa ataccaataa aacatgaatt taaataatca attacatttc ctctctcgaa
      541 aaaaaattag aaatacgcct aggataaata gagggacaaa aagaaaggag ggatagtggc
      601 ttattatctg ccacatgggt gagagtggag agagaaagag ggctgggagg aggaggacga
      661 gggagaagag agggggcgga cgcacaggaa gcacatttgt atgcatttcc tctcataaca
      721 ggaagaggcc agtggcttcc ccttcacaat ggcttcgctt ctgcttccca cctcctgcct
      781 gcttccctgg gggaggagga ggtggaggaa gaggaggagt ggagagacag gcaccaaaaa
      841 ctgctggtta gcaaaccaaa catggccatg cgaggacaca catacgcaag cacaaacaca
      901 cacacacaca caaataaatg tcagtcagct atggagcagc acgattggtc ttgagacaaa
      961 cacttatccc attgtcaggg gccaagcagg aagacacggg cttacgcaaa aggaataaga
     1021 ggagcccaag acctgggcct gaaccctgcc tcacagcaaa agaagctgtt aagaggggca
     1081 ttacaacaaa agaggctttg tttcacctct aatacatatg ctctgctctc tcctcaaacc
     1141 ctacttatca tcctttttta ttatacagta gactccaaac tttcttttat tccagcgtct
     1201 gaatcgaaag tgtccacttt gagtggttaa aagctaatct gtagaactga ttgagtggtg
     1261 ttttaattat gccaatttta gttataggtg tcttaaaata catgccagtt cagactgttc
     1321 ttaccagagc ttgtaattac atttcaggac ag
//
LOCUS       KCVB01000213            1165 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10041pri ultra-conserved element locus
            uce-426, sequence.
ACCESSION   KCVB01000213
VERSION     KCVB01000213.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10041pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1165
                     /note="ultra-conserved element locus uce-426"
BASE COUNT          302 a          259 c          236 g          368 t
ORIGIN      
        1 atttgaagca gatggaaggg ctttgccaaa agcttttgtc tacaacaaac agatttaaat
       61 ctaaaaactg tacatggctg aatcaacatg aaacctttca tcgtactgtg gaactgcctc
      121 tctcgttttc tcttccacct gccacttgtt ttttttagtc tcagtggagg tgggggcata
      181 gaagatgcac tgaagtgaag aggctgagtc aattgtgctt atttgcgtag tttactgcac
      241 cgttagtgca agtagggaaa acagcaaact caatagatct ttcctttctc acagaaggtc
      301 agcgtgtcgc ttctctgctt tttgcgtaac ccggcttgat tcccagtctc aaagcgattg
      361 aaatacagga ggtgtaaaag gggtttgcat gcgcattgat cctctctgaa acaaagtaag
      421 taatggtccc ccctggaact ggcaaggact tttcccttca atgtcacccc tcatgctcaa
      481 tcaccgccgc cctcctccca ctaatcccag cgcagaaatc ccgggatttg tcgatctatc
      541 acttctaatt gacactaaat gatctgtcag tttattgatg aacctttgaa accaggttgt
      601 cacaatatat tttatcccta acaacttttt ccagacagtt ttaccatgtc tatcgctttg
      661 ccttacatga gtcttaatca aggggcctgt aatgtgtctt aagtgtcgcc acccccgccc
      721 cccagacagg acacagtcca agggcaggca ggtaggctcc ctcagctcct ctctgccctg
      781 cctggttgaa ctgtagtatc ttcaaggctg ttggtctgca ctcagacagc atggatacga
      841 cagacaaaac aggagtaaat tggctagcta attctgtttt atgtcatact ttccctttat
      901 ttttggtgtc tctttaaaaa aaaaaagatt cctaataagg taatgacagg tggtgtaaaa
      961 tgggaaggat taagatttcc ctgtttactg aaaaggacat taatgtgtct ctggattttt
     1021 gtgtaagttt tttttttttt tttggagttg gtgagtttgt ttgcttgaac tgaactgctc
     1081 tgtgtcaggt caattttatc tttatagccc aaattcccac atcaaatttg ccttaaaggc
     1141 tttacagttc acatggcaac atatt
//
LOCUS       KCVB01000214            1325 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10083pri ultra-conserved element locus
            uce-849, sequence.
ACCESSION   KCVB01000214
VERSION     KCVB01000214.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10083pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1325
                     /note="ultra-conserved element locus uce-849"
BASE COUNT          346 a          300 c          263 g          416 t
ORIGIN      
        1 ttaatcagga tggagtctga gaaattcctc tctgatactg atcaggttcg agtgtcaatg
       61 ttgattttct ctgacattcc cttacttcaa cttgtgtgct gagcttcatt tgttagattt
      121 tctttgtata tatgagcctc tttttttatt ttcggtgtca tcaactgtac gccagaacag
      181 ccaacacatt gagtcccatt ttctgggaca cagtctaaac cagacttgag catgccctca
      241 ttatgaaggt ttgcaacccc ccgagagtgc ccgacctgac cccaaaaatt gctcaattga
      301 ctttttccat ctgcagttat gtgtggtgat ggggaaccat gacatttaca ttcgcaccac
      361 cccaatatga tcccttgctc ggcgcgagtt tttcgacaca gactatagct caaaacaggt
      421 cacaggatca aaagtgtctc tcgcccggag gagccactga gtcgatcgtt tccatttctg
      481 caccaaatgg tgttttctct ctctctctct ctcaccagta acttgctgga acacatttag
      541 atcctgagta gaactcctca gtgcccactc atcactctgc gtggtgtttg aagattacta
      601 cgctcagaga catcctcact gggcatccag gcagcttaga ggccaaaatc cataatccct
      661 tacccttcat gggaattctc catgcacttt aacatgctga aatacagccc cgctgcagca
      721 gccactcacg gtcgaggaac tactgcagcc ggaggccgag atagatggat gacatgtctc
      781 aatgaatgtg ggagaagtgg aggaagttag atttaaaaat aagttatata ctgcagatag
      841 tttctgccca gaaatccaat cttaaaggat gtttcttgag aggtattttt atttctgatg
      901 tatgcacact tcttcagtcg gtccacatgc actggtaact attttatgta tccaataaca
      961 gcccagagga cattatttac tggaaatata gatactttgg tgccgcacag ctactctaca
     1021 agttgtttca gtaaagcatt tcctttgagg ctttagtgcc ttattaaaaa gcataaataa
     1081 gtttagttcc ttgttttggt agtctgcatg aacaagtaat atgtaaagtt cagctgttgc
     1141 agctgaagga cctgtggggt ctaatttttc ctcctctagg ctacactctg ctgctttttc
     1201 agtctactct gctaaaacca tgtaatagct ttgggagttt gtctcgcagc aaacaagaat
     1261 aataaaagta atctcatatt cacatttggt attttctcat tatgaaagcg tgtttttttt
     1321 agcgt
//
LOCUS       KCVB01000215            2037 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10124pri ultra-conserved element locus
            uce-1038, sequence.
ACCESSION   KCVB01000215
VERSION     KCVB01000215.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10124pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2037
                     /note="ultra-conserved element locus uce-1038"
BASE COUNT          618 a          385 c          502 g          532 t
ORIGIN      
        1 tataatgatg atcgctgata ataacaacga cagtgtcaaa ctatgtcggc catgagtttg
       61 ctgacgttat aaaaataatc acggacatta acaatgcagt tctcaggaat atttactctg
      121 ctcaccagct ggacggcaga gaggcatctc caggttattt ttgcgcagaa ggagaaatat
      181 ggtgctttga ttactggtca gcactggagc tcggggcctt gaggaaatac cagtcagcac
      241 ctccctgcac tagtgagatg gaagataagc atatgttgga gtagctggcc tgggtgaata
      301 gtagtattta gggtgttgga cgtggggttc tggaaggccc cagagccggt ctacaatgag
      361 agccatacag cgccgtctct ctcctgtggc accttggagt gatcagaccc aggcaggacg
      421 cagaggaacg cagctgtaaa ggcaaggcca gcaggggggc tttgaccgtt tactgacagc
      481 catgctggat ccagcaggcc agtgggggct tatttgcatg tgtgtctgtg tgtgtgtgtg
      541 tttgctttat atatatgtaa gtgggtgagt cagtagtttt gctgtctgtc catgcatgct
      601 caaatgtatg cgagtgtatt tctacatcat attattacta atgtataata catacatcta
      661 aagtggctgt caggactggt cagtcatcat aaaacgcgag gcagccactc tgacatcgcc
      721 gccacattaa cgtgttctca gaagaagaaa aaaaaacatc cactgtggac tgtcatgtag
      781 gcagggggga tagagacaga aagacagaga gcaggtcggt gcttaaagct aacaggtaac
      841 ctttctgtgc acctaggaca aaggcagggg gaaaaagaaa atgtcaaatt aagagaggcc
      901 aggcagagag acgtggctgc catatttgtg tgaaagccca gtgctgttgg cagcagagat
      961 ggctgctttc tgattcatct ggggccgtat ggcaaagctt tgtcagtctg tgcgaggtat
     1021 aaataattca gggtgagaga tggcaattac agggccctgc acaaccaaaa tgaatatttt
     1081 ataaggacta aaactgccct ggaatgaatc acacacatgg agtcacacac acatacacac
     1141 acacgcgcac acacaagcat aagtgggtgt gtgcacgagc acacacgtaa gcacacatgc
     1201 acccaaacac acacacaaag gtagcacata tgaaccctta gcatggctgt tgtattgttg
     1261 acaatataac tattatatta tataaagctc tgcattgagg gttaataatt tttaatatgc
     1321 tttcatgagt gatttctcac aatatttggt taagggggaa ggagcctgtt agatttgttg
     1381 tttcgttatt ttgttgttta aaaaaaaaaa aaaaaacaca aacaaatata ccagttaaga
     1441 ttttaatatt cagtagcccc atgtgaaatg catttgcaga aaacaaacac aacaagagaa
     1501 aaaaagtgtt gtcaatatat agtaaagagc tacacaaaaa gcagtctcca gagactcaca
     1561 gcctttactt ttgaccctgt aaatatggtc tttgtatgtg agcaagaggg aggggggaca
     1621 aaatgctaat gggaaaagat taaacccaaa agccgcctgg agcatttacc tctaaagggt
     1681 tttgtctgtc aggtttcctg caaaactctg tctttataat tcttagacaa aaaataacat
     1741 tgttccctag tcctgaggag cgcagtggct tggttgaatg gccattgtgt gggtctagct
     1801 aatgatacct tagcttttgt gagagggagg ccaggtaggt ctgtgacatc agcgccacac
     1861 aagtgcttct ctgtccggat gtttagggaa ggatttcgtc catggtggga aaagggtata
     1921 gagaataaac aggtcctgtt tttgtaccag cgttttgagc cccactgcca aagaaggggt
     1981 tattctgaga aaaaaaatgc attcaaaata acttttttta ttttaaatca ttaccat
//
LOCUS       KCVB01000216            1446 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10160pri ultra-conserved element locus
            uce-762, sequence.
ACCESSION   KCVB01000216
VERSION     KCVB01000216.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10160pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1446
                     /note="ultra-conserved element locus uce-762"
BASE COUNT          406 a          300 c          285 g          455 t
ORIGIN      
        1 atgttgatat ataatgattt atcaaagctt attctgtctc tgtagagaag acgatttaat
       61 tattattgtc atatcacaaa acattctgaa actgactgtc caacattact gctctcacca
      121 gtaagcatgt acactcatgt ataaacataa ttacttaagg tctttgccta actggtttgt
      181 cccgctggta aattcagcat ttgaataatc cacaaagcag cttagcgcta tacaacttta
      241 tcataggagg tttcttcatg tattacatgt gcagcaaatt taacaagcca ataaaacaac
      301 ttgaagaagg ttgtaattga tgttgcagga aaaatttatg ttgctgacga ttatcattac
      361 attggtagat ttcacaatgt gttttaaata acagcacaca aatgggcttc agtgcaggtt
      421 ggaatctgtg ttgctctgat aatcttaaga ttcttcttct catacagaag tatggagctt
      481 atacctggtg tgttcttatc agcaacataa ggacagaagt gtattccccg gcagagagaa
      541 gttatttgac tgaattccag accctaacat gccaatggat tacgcttctg gcaagcagct
      601 ttagtagagg caagcgtgta cgaacaattt cagcgctcgt ctcctgttgc gacacggtac
      661 ctgctcatac ttctccagct cagtgtggta gatactgcga atctggctca acttgctttt
      721 gtagtcggag tgctcgagtg agctgtcagg tgacatccct ccagagctgg tggcagcaga
      781 cacagcagca gcggccccgc cgcccttctc cggccctgcc acaccctccg ccagcagcat
      841 gttgtccagt cgcacaagct gagggtcctg aggctcgtcc tcctgggtgt ttctcattga
      901 caggcctgtc aaaaagataa atgtcactgt ctattagttt tcggtgtgta atgtcaacag
      961 aggactgtaa atgtgttaac gtacagttac atttcatttc tatcctgtga acattcctgc
     1021 catatcctgt ttgtttcaac tgaagcctgt acactcattc atgcctttct tgttctggtg
     1081 ccactctggg gtaagagtta atttttgttt aaatttgtcc tgtaaataca cagggtcagc
     1141 caattgaaac actaatggat cgtgtttgga aatggacaaa tcatctcgtg cattagtctc
     1201 atatactgtt tacagtttgc attaactctg cacaacactt atttattacc catgtcaaaa
     1261 catccttttt cggggcagga cagggcaaat tcatttgact aaaaataaat gtgatcgtaa
     1321 cttttcagtg tctttgaggg aatctgtttt gaaatgtttg cctttaagaa gcagcttgct
     1381 ttgtacaaca aaggatcaaa ttaaatgaac ttttataaca taaataaaaa tttattacag
     1441 cattac
//
LOCUS       KCVB01000217             689 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10198pri ultra-conserved element locus
            uce-727, sequence.
ACCESSION   KCVB01000217
VERSION     KCVB01000217.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 689)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10198pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>689
                     /note="ultra-conserved element locus uce-727"
BASE COUNT          211 a          129 c          123 g          226 t
ORIGIN      
        1 agtctaactt ctcccacact tcaactcccg tctcctgatt gtggtactct ttccattgtc
       61 tttgacacaa aaaaagtttg tttatatatg catgtatata tgtatataca tatacatatg
      121 tatatatata tactgtatat aaatacattt ttatagctac agcacctttt cccatggggc
      181 aacagtgtca atacctcgca catggtacat ggccgtgcca aagaaaaaaa gaaaaataaa
      241 aaaacaatat attggtcagg atatttatct ttttttccct aaaattggca tttgacatga
      301 tgatattcta ttgccgtctg ggggagggaa gggacaaagg ttcttctttt gagtttatat
      361 tcaagttttc tcctttttct ggtgtataat ttcttctttt cctgtttaaa aagagttcac
      421 acttttgtga tttgcgtgtg ctgctcttca tcaaaaggtt cattttccgg atcagagtca
      481 gtggtgtcag agtatcgata atggtctggc tcattgtcgc taacgtccgg ggtgaccgat
      541 gtcgaagtgc ttgcctcaga attcgaaggc tcctccacgg ttttcgtaaa atacaacttc
      601 acctggaaat gcaaattaaa actaacttca gtctgtgcaa taaaaaagaa aaaaaaaaaa
      661 gaaaaaaaaa gagcactgag cgctatctc
//
LOCUS       KCVB01000218            1638 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10281pri ultra-conserved element locus
            uce-954, sequence.
ACCESSION   KCVB01000218
VERSION     KCVB01000218.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10281pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1638
                     /note="ultra-conserved element locus uce-954"
BASE COUNT          511 a          347 c          318 g          462 t
ORIGIN      
        1 tatattctgt tacgatttca tgctagccat ttacctgttt ctagtcttta tgctaagcta
       61 agcttaccac ctgtagcttc atatttacca tacagacatg agaatggtat cgatcacgca
      121 aaaaagcaaa taagagtatt tcccaaaatg ttaaagtatt cctcaaggtt tttgtcagat
      181 tgcatcattg acaccgtcca cccatcccta aatgcactga tgatgtggtg ctgcgtttga
      241 agtttcacat tcaaatatat ttgatcattt cacatctctg catttctcca tttttataat
      301 tccaaaggag cattaaggta aggtttactc ttgccgagcg caatctttta acttggagag
      361 tcctgtcctt catctcttgg ctctctgacc tttctgtggt gtgcaccact ctggaaagtt
      421 agcaaccatt ttcacatcat gtttctaacg ttagacaaac agacagatga ataaataatc
      481 atagttgacc tcgcacaacc ggggacagcg gtctaacacc tccactggca aaggttcacc
      541 aaaatgccac tctgctgcca ttatagaaga aaggggggaa aaaaatggca ggaagaaggg
      601 atgagaaccc acaagaggtg gacagtgaca aatggtgtgg aaaaaacaac acaagcagcc
      661 atctgaagga aagacaagag taatactcac aaataattaa ctgggtaaat attggagagc
      721 agactgacta attacttcac acgtctcact gcgctctcat tattgttgct gtcacagtta
      781 accctttccc acaaggcgtg gctgtgcaat gagctcttta caggagagtg gatgaatcac
      841 ttacctctca gtatttttat ttattcatgc agttggaaaa cagagaagaa cacacactgg
      901 agggacattt gtgtgaggat ttaagccaag gccagtcaag agaaccatga gagaaaatat
      961 tttagagaac aatacaggtg gctttgtttt agcttataaa ttacaaaata tgtaaaggtt
     1021 acactgcaca ggttactgcc tatcattttt ttttatcatc aaacctttta aatctaaaac
     1081 actacatgct tttcaaggca gcaaatccgc aaacaaaaca aataaaagga tagagaactt
     1141 atctggagcc tgacaagtgc ttcagcataa aactttggtt tataatatcg tgttgcaaca
     1201 catcgagccc agctgatgca tacccagcca ggatgactca gctactcaga agttgctcga
     1261 tctacaacaa agaagagctt tcagaaagtc atatcacaca aaatttgttt gaatcaattc
     1321 gaaagcaaag caaagtgtaa tgcacagcca cagttctgct gttatcttga aaacattttc
     1381 ctgaaacaag gtcaattggc agcatatcct cgcctaaatc ttgccatctt attagtgagg
     1441 gcctttttac aagcctctgg tagggatcct ggggatcctt aatgtggcaa agagtaggca
     1501 gtcagaatag ttgtgcaact gatgttgctc cacttgtttc tggtgcctcc gctgcctcac
     1561 ttgggtaaaa cactagcaga tttgtctaag gcagcagctt cttacaaaac agccttatct
     1621 ttgcctctat tgtcgagc
//
LOCUS       KCVB01000219            1430 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10321pri ultra-conserved element locus
            uce-583, sequence.
ACCESSION   KCVB01000219
VERSION     KCVB01000219.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10321pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1430
                     /note="ultra-conserved element locus uce-583"
BASE COUNT          332 a          384 c          308 g          406 t
ORIGIN      
        1 tcctggctta ttgcatcatt tgtggactgt ttggcttgcc cctgcaatgc ctcgatgttt
       61 gcagcctgtg tgtgtgtgtg tgtgtgtgca ctcatgtgtc cgttcatgtg tgcatgcatg
      121 caaacatatg tctgcgtgtg tatcacccct gtgtaaccac ctccatgatg ggctaattgg
      181 tttagcagcc aaagggccaa aggccagtgc agcatagcaa aactcaacat gaacctgatg
      241 ttgaagggaa ggcttccccg ctataggagg gaagcatgtg tgggacaatt ccctttttac
      301 caccccctcc cacacgcaca cactactttc ctttttcact taaagaaact cagagcataa
      361 gaaaacaaaa ttcttcacag tgttctgggc cgccgtgctt cagactggag tagcactaga
      421 ggtaatctct tccattttaa catattttca gaccttcttc tgacgaatgt cacatgctat
      481 tacattaatc tagaatattg aattggaagc ttttatgcac tatgaataat atacacactg
      541 tgatattcag ccgagcctca acctgcagta tcagcgggcc cctcagtaag gtttgtggag
      601 cctactttgt gtggggatct gaggggatca tttaccagtt ccaactccat tactattcat
      661 gagatgggta ggctaggggc gagggattag ggactccctt ggtgcttgtt gtgctgtagg
      721 ctcgggctca ggcctcaggc tccaggacag gggatgtctg gcacggatgg tgggtctatt
      781 atcaccctgg gacccttctg tcccacagca gaacacttac tggcagtgcc agctaatgga
      841 aacagtctct cttgttccct tactcttttt ttaacctcat caacctcctt gtatccttcc
      901 tcctgctctt taaaaaaaaa aagatgggga agtttttccc tttccctatt tcaaagttga
      961 aatggtgtat ttctattggc atctttaccg tgaagtgcta ggatggctct atatttgcag
     1021 gaaaaaagta ctttccctca actctgtcaa tgctttgatc ctcaattttt caaatttcta
     1081 acatccttcg ttcccccttc ccccccaggt gatgagtatc ctgagcaacc ccagcgccgt
     1141 accctcccag ccccagcacg acttttccat ttcccccctg cacagcagcc tggagagctc
     1201 caaccccatc tcagccagct gcagccagcg ctctgacacc atcaagagtg tggacagcct
     1261 ggcttcctcc cagtcatact gcccccccac ctacagcgcc accagctaca gcgtggaccc
     1321 cgtcactgca ggctaccaat atagccagta tggccagagt gagtaaacct gcattaacat
     1381 ttatattctg tatgtttgcg tttagcttga gttgttgcag aaacagctgc
//
LOCUS       KCVB01000220            1260 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10362pri ultra-conserved element locus
            uce-152, sequence.
ACCESSION   KCVB01000220
VERSION     KCVB01000220.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10362pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1260
                     /note="ultra-conserved element locus uce-152"
BASE COUNT          385 a          278 c          237 g          360 t
ORIGIN      
        1 cacacacaca ccgagctaca tgacatcaac ttctgagagt taagagactg ttattcacct
       61 tgcaaagtta agtttttggg tgaagcgaca aaagacatat ggtcctcccc taaagtactg
      121 tgacaataac agacgcatct cgaaacacat ttcagggcga cacacctgtt aacataaaaa
      181 caaacacaca cacacacaca cacatgcaca atcactttcc aaattaacta aagactgatc
      241 ggggggagct ttatttttgg ctattttaag tgtgaagaag tttttggaat aaaagcttta
      301 aaaagtacaa ataagctttt ttcctctcat gtaaatgtga agaaaaatat aaaaagaagg
      361 acttaaactt acacaaaaca acagatgcag tgggattttt tttcattcta atattaggga
      421 ttgtatggac gtatagcaac atgtttccat taaatgttct aagaataggc cacagcacat
      481 aaagcgaaaa tttacaaggg tgtcagtcaa caatttaata aattgaaaat ccatttatct
      541 gaatacagag ttctgccctt cactccactg cctttcccta acagatggtg ttgtgggtgc
      601 ttatgtccaa tttgatttga accaaaccct cagccaattt ttacactggc atgaaatctc
      661 agcgagccag atccacacac tggcagtcag aaatagcagc ggagagggcg cgacattgtg
      721 aaagaacgat gaagggcaaa atctggagcg acattcgaga aagtcgcaga aaaactttcc
      781 ttgttgtttc attttcgctt gtgtgccgtt tttttttctt cctttctctc tctctccctc
      841 tctcccctcc tctgcatgtt gtttttgtgc cgagctggag aaattagcga tgctatctta
      901 atgctgtgtt tttgcgggag acagagaaat gttcctttca cacacacaga catatacacc
      961 attttcccaa ttgcctctgt gacctccatt atcattaaat ggtgtttgat agcatctaga
     1021 catttgaatc cgctgtggac taattcagct cggcccctga tagccgctgc cactgcacct
     1081 tattagggac aagctatggg accgaacaca gatcccattt tcattttact gcctcgtact
     1141 ttacaggctg aagagaccaa gtgtcaactg tggtactgat aaccaccaaa tgctgcctgc
     1201 taattgcctg gttaacaaac agagcctcat gaggcacttt tgaatagata atagaggttc
//
LOCUS       KCVB01000221            1607 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10406pri ultra-conserved element locus
            uce-911, sequence.
ACCESSION   KCVB01000221
VERSION     KCVB01000221.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10406pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1607
                     /note="ultra-conserved element locus uce-911"
BASE COUNT          476 a          365 c          286 g          480 t
ORIGIN      
        1 catataaatt aaattacctt taaagcacag atttccctaa aataaaaaat tccattttaa
       61 gtagcattaa atatactgta tctttatatg aaaatattac tcttttatat gttaatttta
      121 cggtaatatt ttgttcctat caacaaaatg acaccaaaga aaagtttgtt cgctccagat
      181 ttaagtctag ttttacttct gtgaacaaac aatacaaact attttcaaat atacccagta
      241 tgctgccatc agctgtatgt acttctcaaa taatccactg gtgaggcaca gttattaata
      301 aacgaggcag gccacagaat aacatcctcc aggttgcata gtaaactgta tgaaaaagct
      361 ccttttgtga aacaggctct gaacaaggct gcaggacatc accgtgttgg ttggattact
      421 gtgtctagat agtgtggaaa aaattgcatt acattgttgt gcctgttggt gttttcactc
      481 agaaatactt ccatttccaa ttcttaatga caaacaagaa agcttttctg attgaaaaca
      541 attccagcac ttcaagccat aacaaaaatc atgacaagag cattttcacc aaaaggaaga
      601 catttcacgc actgggagcc ctttgtaata ttttctctcc ctccctcttc cctccctctc
      661 tctccctgtc tctttccttt gtgtgcgtaa atcatctctt ccctgcggcc tgtctgtgat
      721 catgttgatg ggaaacaaag aaatcatctt gaatggcaca ttaatttcct ccgttctccc
      781 cctgccctct gcctaatgaa atcccctctg ccacggggcc tgaggagcca cactggcaag
      841 ccgtcaccag ccagtaaata tgtttacctt ggagcactac gctaatggac tgtttactag
      901 ggggaggcag catggggtgt tacagagacc aggcagcact gggtctgggg tgtgtgttcc
      961 tcagactaaa tgacagctcc tttgccccca cctcatctcc caaaaactaa caaaccccca
     1021 accacctgat ctagagtggc tgtcaccaaa aaggagttca gacagagaac tataatgcaa
     1081 agaaacatga cgggattact ccaaggtaaa ataaatctcc agtctgattt ttgtactgct
     1141 gtacacagtg gttggcaggg ttgtgttgtg gactggatga cagtctccta acaaagttca
     1201 ggtccttttg tcagtgagcc tttaacatat aaggaagctg ctggtgtgac ttgtgaatca
     1261 tttaccagat ttagttttac atttcatcca aattatttta gaacacacat aacacataaa
     1321 accgcatcca agttgtctcc ttacctattt ttgctccaaa atgcttgcca aaacttaaaa
     1381 atctagttca ttttaaacct agcttgcagc cctgactaat actgtaggct cttaataaac
     1441 tgtcttctct ccaagtcacc aaaccttcac ctcagcaagt ctgtccagtt tcagtatctg
     1501 tggtgtaacg atgcaattta ttcaggggtt agaagataaa ccttctctct ggtgtattat
     1561 ccaaatacat acaggaaatt gatcatgggc aagctctggc tgaaggg
//
LOCUS       KCVB01000222             923 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10449pri ultra-conserved element locus
            uce-1160, sequence.
ACCESSION   KCVB01000222
VERSION     KCVB01000222.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10449pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>923
                     /note="ultra-conserved element locus uce-1160"
BASE COUNT          273 a          190 c          245 g          215 t
ORIGIN      
        1 tagatagata gatagataga tagacgtgca aaatgtctca agcctatggt caaatagggg
       61 tcaataaggt gcagaggagt ctcactaatg tcctgtctct gccgggccaa catgaccact
      121 gcaggtcttc aggtgattaa catgttggca ggggttccta catgaaaaga cccctgtttc
      181 tttgtcttct ggtgtgtaaa tattgtgcta gcggaatatg aaagtagtgc aactataagg
      241 tacaaaaaca tagggaaaaa agatcaagag tgggtctcac atcaaggagg accctgagga
      301 tgtctttgtt gtcaacttca gaagggggaa gagaggagca gaggaggaaa ctggcagaag
      361 ggggaaagat gccagagaga gctcaaagga ttcggtctgg gcaaggtgat cggtattcct
      421 cggcagtctg ccttctggct cagatcagtg ggagtaagct ggcagccaag ccagagggca
      481 taacagcaca aacacacctt cagcactaca ctgctagagg cttggcttca caatctggag
      541 gggcgggagt tggagggaga gacagaggga agagcaggaa aaaataaata caaataaata
      601 cactgtaatt atcttcaaat catcaaccct tgacatccat tgtaaatatg tgcctgtgtg
      661 tttgtgcaca tattgcagta aaagccataa cgcatcacct actgttgcat caaaacaata
      721 gtttattgga tgagattcgg ccgtgggtta tgtgtggtga aggcactgag gtcatcagtc
      781 aggcagactc atcctcaggc cagcgatgtc gtgtctactc acatcaccac ctctctcttg
      841 gatgaaatcc aaaaactctc catgtgtgac gcgtggagag acttcatccc tcttcaagga
      901 cttcctgccg ccatgctggg aca
//
LOCUS       KCVB01000223            1726 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10495pri ultra-conserved element locus
            uce-1125, sequence.
ACCESSION   KCVB01000223
VERSION     KCVB01000223.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10495pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1726
                     /note="ultra-conserved element locus uce-1125"
BASE COUNT          437 a          464 c          361 g          464 t
ORIGIN      
        1 ttatcagtct ccccggtatt cagtattggt gggatcggag gcccggctgc cgagcttcac
       61 tgtaaagtgc tcggctgatt ggaagggaca tttttccaat ggcgctgtgc tgcctctaat
      121 attctgtttg tcgttgggtg tgaggctgat ttatcacgcc tcctcgcctg ctgacattca
      181 gattcacttt gattggcggc tggacggaat gacacccacc accaccaacc ctgctaacat
      241 cactgcggct ctcacacata tgcacaaaca cacacgcaca cagtcaaaag tgatgttctg
      301 catactcaaa gtcaggttta cagtggatat gaatatgccc actacgccaa cacataccca
      361 cttacatttc gacgtgaaga catgcacttg catataaata ctagctcaaa aactcacaca
      421 cagactaagt ggataactgt tgttctgacc tctgtcttct gcattgctag tttgtaagtg
      481 ttgtattgag tttattgaac aacgtgggca gactgctgct cttctgttgt tctctcacct
      541 cacgaactct ataatatgat agcctttctc acttcaatac ttactgtacc cagcctaagg
      601 cccaagctac cacactcctg tgttgaataa catgtctctc ctttttctca ccctctgtct
      661 ctcccctcat cccccccatc ccttcttaat aactctataa ctctactcag ccttagattt
      721 aattgcttat ctgtgtgtaa acgtattaac cctctgctgt atctgttttg ctgccgtccc
      781 gggggcccga caagcctctc tgactgccgt gccttacatc caatgaatag tccttctctg
      841 agtggtaata gggaatcagc atcgtagcag caagcattgg cagcgtgtaa gagataaatc
      901 agattggggg tcttgctttg ccagtttatt gaatctaaat tgcgtgcgtg cgtggccccc
      961 gccggcccta cacggcccat ccatctcccg gctgataatg gagcgggccc gattccacct
     1021 ggtgggagca ttattacatt tcccctctaa cgagacccag ctaatcctgc caagggagga
     1081 ggacaggaga ggaggagaga aagagaatag aagaggggga cgagagaagt aaaggaggat
     1141 gttgggggag gaggagaaga gaagagaaaa aaaaggagga gaaaagagga gaggagattc
     1201 tgtaacacaa aaaaatttga aacatgctgg cagactgcct ctccctccca ctctccgtct
     1261 ttctctcttt ctcactttct ttctctctgt tgccgtgcat aaaacatctt ctagcgccat
     1321 cagagctaca tggcccctgg gcccctgagc tgcaattaca ctcataatca caatgcgatt
     1381 tcaccgacgc tctccgcccc ttcctgctgc catccctcct ctttccctcc gcccctgcag
     1441 ccaagactga acgaagtcta aatgaagtga aaggcctctc ctctccccaa cacccggccg
     1501 tttattaccc actctaataa aagtccatca cagaattatc ctacaatgaa ctggagccac
     1561 taagttgcca agaaatatac ttccaataag cagcctctgc acacagacat gcctttattt
     1621 ctggatgggt gcagctgtga ggcttaaatc ttttacatgc tttttaccac tataatgtta
     1681 acaggctttt catgatagtg cagcaaagtg cgttgctgta atgtat
//
LOCUS       KCVB01000224            1755 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10535pri ultra-conserved element locus
            uce-216, sequence.
ACCESSION   KCVB01000224
VERSION     KCVB01000224.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10535pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1755
                     /note="ultra-conserved element locus uce-216"
BASE COUNT          534 a          353 c          330 g          538 t
ORIGIN      
        1 ttctttagaa aataacgttc aagatcattt acatgaaagc ggctgaactc ggtgacggtg
       61 aactcgacaa agcagcaaca cagtctgtgg agcattttga cactaaagac ttcggcaaac
      121 agtacagtat aatggagagt tctttgtctc ctctgtattc actgtatgct gaatatctac
      181 cctgaagagt actggaggag gcagtgaaat gagggttaga gtgagcttga ctcccttatt
      241 gctaagttca accaaaagag ttgagtgtag aggctaaata agccaaataa ataatgctct
      301 gtaagggctg aacttgaact gtctgagaaa acacacacac actgtaatga ttttagaata
      361 caaaaaataa ggtatttttt ttgaggacag aatttaagtg cagcaagaaa ggaaaactta
      421 aataatgcgc ttggattgca tttgatgttg tgatatttca cattgtgttg gctaacaaca
      481 gttttcagaa atgtgtatga caaacaatat tagcaaatac atggggttgt gattcccttc
      541 aaaccacagt agtataggga gatgcctgct tattggcaga gggaataact tttaaaccgt
      601 gtcccagttt ctacagactc cagcacagca ttttatgtca ctatgtccaa ccctatttca
      661 catcctagtt aagtacactt acaaatctgc tgtgccatgg tttctaacct ctcatacaca
      721 tgtgaatcca cactagcact cacacacaca cacacataga gagagagaga gagagtcaca
      781 tcactaggcc tgtcactgcc ctttatccat cgtcaacctt cagctgtatg gtttatccgt
      841 agtgacacta tagcaaagtg cttgactgct ttttgccaac agactgcaac actttaactg
      901 tacttagtca tgcatcatta tggcagactc aaagcggtgt gtctgggaac gcctgcagat
      961 ggtggaggct gaaccatttg gaaaagaagc ctgtgtgaca atgatcctta ttgcatgagt
     1021 gaatgtgtat ggacacagga gtgtatctgt gctaaataga aagcaaaata taaaccaggc
     1081 acttgtgcta attcaacagg cttgagaaaa cgttatctgt ttatgagata tgatatatga
     1141 aatctacatg cgagtctttg ttagaaagat tcttttatgc gtgtattgat cagtcattgt
     1201 ttacaccaat cgatgctgac ttggtgaaat gcaccctcct ctctgttctt tttagagatt
     1261 taaccctctt cgcattgcgt tctgctgctt tgttcttctc ttcaaacctt ccttcagtct
     1321 gaaaatgtga tagcattact gtcacttgtc actttctctt tatttcaacg gatgacaaag
     1381 cttttacatt cagtattctg ctcctcacac gggtattttc aacagatctc gtctgctatt
     1441 gaaagataaa tgttaactcc attcaataga aacctcattc aaggttcatt caaagctgac
     1501 ttctttattc tttaaggaaa tgaagaaacc attagcaaaa tattcactca cggagaaaga
     1561 gaatcttatc tttattccgc atgaccatgc agagccatat tccttttgac atcattatgt
     1621 aaaaccaatt tctctttcat atcatttccg ctgtgctgca ttaccacatc tatacactca
     1681 ctattcttta ttacaaagta ggtggcctag aggaaaatat atgcattcgt tccctatggc
     1741 ataaaatatg catat
//
LOCUS       KCVB01000225            1793 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10577pri ultra-conserved element locus
            uce-253, sequence.
ACCESSION   KCVB01000225
VERSION     KCVB01000225.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10577pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1793
                     /note="ultra-conserved element locus uce-253"
BASE COUNT          499 a          427 c          381 g          486 t
ORIGIN      
        1 ggttttctga atccactcta taacagactg gaaatgaccc atttgtatga aggaaataag
       61 catatttgat ttgtcagtac tttgaatgga gtcattaagt actgaagtca tggaatttat
      121 gctaattgtt attgatgctc ccccatgatt gccaaataat aaccgatgat atgctcctca
      181 catcatcaca tttacatgtt gctctccaca tcggctcgtt agcgacagcg ccgtgacgcc
      241 gggcctatcg ggatccactc ccaccacctg cacacttgga gtacgggagc gaggggaccc
      301 agtgtctgca gcgtgccatc acagctttgg caaaaagagt gccgtcgcag ccttgtcacg
      361 ttgagtcagt cccgcctcgt ctctttcctc tctccatctt cctttttcgg tcaggtgatg
      421 cacacacaga aaaagctcgg ccgtctgcat ccggagccct aacgctctct gtcagctggc
      481 tgtccctgcg gtcacagtgg acattcgtct ttctgctgac gtccctcctg tgtggcaggg
      541 agttgccagc cccagcattc ttaaaggcct gccatggcct caaggcagag gcagcacgta
      601 atggtaggta cgtcaaggca acgctgatct gttagtaaat gatcagaagg ccaaataacc
      661 aaatgattgc cttctggatc tgattcctat gctaatcaat caacaaaatc tatatcaaca
      721 cacacacaca cacacagcat tacagatact aaaacactgg aattttttag gtttctcttt
      781 tctgttgttc taattaatac gccacgtctt gggcacactt gaggtaatta acattgtttt
      841 ttgccagcaa cacacagcag atggtgattg aaaatagagg gatgagtcgc tcaatcaacc
      901 accatttgtc tgtagccatt aattatctag cgaagaatac attcagtccc caaagttgat
      961 agtttggggg gaagaagaat actgtccttc tgttcctcat tactctttaa cagcagaaac
     1021 tgctaaaact tgaatgccga atagggagat tgcgatttta atagcacagt cattatcttc
     1081 ccccgtaagg tggcatacga tggaaggcat ggagctaaca aagacgcgtt ccaacaaatg
     1141 gaagccaagc aacgtctagt gggtcattct gttctgattc atcaaaaacc actttcagag
     1201 atgatgggga caaaatgcac agtttcattt aagacgatgg gtccagaagc ctcatgaagt
     1261 gcttatttcc cttatgatca aatgagagcc aatgtgtcac cgctggaaga gaactgcatg
     1321 tgacttaaca agtcatccca agtttgtaca tgcaatcgga tcagaataac agcctctcgc
     1381 ctgcagctct tgcagtcatg atatcatgat ttctcctctt cctcacttct tttttcttgt
     1441 tggttttgcc tcacttgagc aaaccaggag cgctgaattc tgcctggcta cagattagta
     1501 ttacacactc agcttatata aaattctcta aaaataaaaa ggctacaaga gggacagaga
     1561 gaaagagggc aggagggaga cagaaagtaa agaggagttt ggggattgag caaagaaaaa
     1621 ccttgaaggg acactggagg caagaactca gcgacatagc ttcccatcct ggcatcactt
     1681 ctttgatatt attttgtcat cgccgacagc tcctgaatcc tacatctttg gcacactata
     1741 atcttgtttt tctcctctct gccagccttg tagtcagaga taatcagcat cac
//
LOCUS       KCVB01000226            1540 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10623pri ultra-conserved element locus
            uce-682, sequence.
ACCESSION   KCVB01000226
VERSION     KCVB01000226.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10623pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1540
                     /note="ultra-conserved element locus uce-682"
BASE COUNT          373 a          328 c          336 g          503 t
ORIGIN      
        1 gatgagcggt agcctatacc tggtgtgatg agacgcgata atgggtttat tagtctgtac
       61 acataggaac ttgaacttaa cgtggaggaa aatgtagaaa ttcaaacgac atggtattat
      121 tgtttttttt tcacgtgggt gaccctgtgt acatgtgcag agaccaaata tttcttctca
      181 tgctatttta tttagaattt ttgctaaata ttgtcgatag tcttgattta aagtagccta
      241 tcacactaaa tgtgtgttta ttttttttcc tcctttttta ctttgtgttt tgtttaaacg
      301 tgtagtctgt ctttatatcc acatgcctaa aggattaaga taatgacaca ataggtagac
      361 agttgtattg taatgagcac acatcatgtc cagaaggtgc acctgtgtgt cgaggacaaa
      421 aatctctttg gatatttcag cttgttgtac ccagctactg caacgcgata tggcgcaaag
      481 aactgagatt ttgctttttc ttcatgaaat ggctaaggct tttaccgtgc gtgcatgatg
      541 agaatagcct atagtctggg caaatagagt gactagaaat gattcctctt tcccgaaaat
      601 cgtcttcttt taggaccaag cggccctctc gacctccatg ctggctctgg caaggtacca
      661 agtagcacaa gtgccgacta gccaatgggg ttcaggggga ggtccctgag ctctgaccag
      721 tcaaacacac tctccttcct tatatggcag ctgatctcca tggtaacgtg tgctaagttg
      781 cagcagtcgt gtcaaagttc actatataga gagctcagtg agctgatcag agagaaagca
      841 ttctgccagc ccggctccta ccaatcgcaa atcacttcct aacctccgcc tttttaacat
      901 atttgatcac tttgattctc tactcctttt tcctattatt tttcagtcca cggaggaggt
      961 ttgtaggttg tgatctttgt tttttgtttt tttgttttag tgctttcgct tttttggggg
     1021 gagagttgtc ttcatattcc aggctctcaa aacattggct tcagctgcgc gcttgctggc
     1081 gctacctcat aggaagaaaa ggaagaagac gcaatttttt tacccagttg gtgattttct
     1141 tcaagacttg tgctggaact tgatagtagc tgctacagtg tgctcaccaa ttgttttatg
     1201 ctgctgcagg cacggtctta ccaaagctgc tccatcattc actgaacagt ccatgctacc
     1261 tcggttgatt attttaatat ttagactacc tttcccattg atgggtgtac tggatggctg
     1321 actgtaagcg cccctggact tggcctttaa gcgcaaggca gcgactcagc tcgccatcgc
     1381 atcagttttg aaaacgtgct tcgcaacgtc tccaacagca ataaatcgac agtttttccg
     1441 gagatccaga gtggattcaa cgtaggctag atttaacatt ttgtgtgtcc ggctgttagt
     1501 cactgtttac cgcgtttcct ttgagacatt atccaccgcg
//
LOCUS       KCVB01000227            1440 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10670pri ultra-conserved element locus
            uce-1224, sequence.
ACCESSION   KCVB01000227
VERSION     KCVB01000227.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10670pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1440
                     /note="ultra-conserved element locus uce-1224"
BASE COUNT          400 a          276 c          340 g          424 t
ORIGIN      
        1 atgcaaacag cagcaaaggt catcaggttt cacgcatgct tggttatgca gaaatgactt
       61 tgagcaacac acagaacttc aaaaacactg aaaatatgat ttccccattg tttgtatatt
      121 taaagttatg aagatgattt gtttcttgtc tagaaagtat tccaaagaaa agggcattaa
      181 aagttctgta ggtggatagc tgtggagaaa ataaattgaa tttcaatatg catttatttt
      241 agttatacac tttctttaag ttgggaaatc attttcaaaa aaaacttata tgttgtgtag
      301 catgcgctcc ataaccaata tcaataagcc tttcatgtag agctaacaaa gaggcatcac
      361 atctcctggc acggacacgg tcggtgttgt tgagatgtaa ttgaagctgc aagggtcaca
      421 tttttggaat agaaattaca gtcttgtctt tgacttgtgt ggcgtgaacc agagatgcat
      481 cccacagccc ttgtgtcctt cacaagcctt tattatttat aacgctcgct ctttgagcaa
      541 tgaccagtac taatgcataa ccttctgtcc ttctggctga ttcgcttcct ttgaaaccaa
      601 agattgaggc aggagaagtg ggtgtatggt ggatgaggca cgggtgtggg cgggtagagg
      661 aggggggaag ctagtatagg gcagtagaga tccaggctat cgagaagtta ttatgttttt
      721 tccctttata tctgaggatg tggaataacg ctgtgatgat tcttttctcc agaatagagt
      781 tatgtgcttc aaagcaatac tatttcctga cgtccctcct tccactattt gtctgctcca
      841 caagaataat gtgctgggag gggtggggtg ggttgagtgt cttttaccta catgattatt
      901 tgcttgtttc cacctttacc cactcagttc tgtaaatatc actgcaacag gggaacacag
      961 taccaacaca tacaggactt tgtccaggta aactcatttc cggataaact ctgtttagct
     1021 caggttcagt ggaggtaaag atatactctc gtgcagcttg tatcacctga tgttatgcta
     1081 tagtgattga gcctgtgctg cagcgctggt gtgatccata gttattatac ctctatgaca
     1141 gaaggaacgg gtgaggaggg ggggatgaca ggaggctttg agggtcaagg ggtagaaact
     1201 gggacatagg cagcattagt gccgtatagc ctggggggcc ctggctgatg tgtcaggcat
     1261 gacatgtggg tgtctagccc cccacgcact cgcaaacaca cacgctgact gctctcacac
     1321 cccactggct acttctaaat gtcctgcagt tgacgatgca acgcagcatg gacaataatc
     1381 aaaaaaaaaa atgctatatt ttaaatgatt acatttttaa ggttacctgc aacttgttat
//
LOCUS       KCVB01000228            1154 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10705pri ultra-conserved element locus
            uce-1059, sequence.
ACCESSION   KCVB01000228
VERSION     KCVB01000228.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10705pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1154
                     /note="ultra-conserved element locus uce-1059"
BASE COUNT          365 a          216 c          299 g          274 t
ORIGIN      
        1 cctcatgagc tacagccacc cctacacccc tgcagtcata tactacggct tgtagcacat
       61 attgtatagc attaaaatct agtatgtcac accaagtgtc tcctgtaaga aagagaacag
      121 tggagaagga cactgacgat gatgaggggg atgatggtgg ccgctgctcc tggattgatg
      181 agatatctgt cagcagcatt aagcagcatt tgggtctccc caggcctcat tagtactgga
      241 ggaactagaa gtagagagtg tacaactggc atcatgtgca ggattacctt ttatactacg
      301 taactaaaag gagacagagg cagaggggtg gaagtagaga gcacggaagg aagatgaaag
      361 aggagagaaa gaacgacaga caaagcagga gagcgtagcg tggcgctcat tagtaaggtt
      421 gcacagcgtt ttgaatttgt gaggaggctg acgtgtttaa ttcttctgag tgagagggtc
      481 tcagtggaaa gttttacaga catgcaagtg aggctggaag agatgtgatg gatggctgag
      541 atgagtattt agactgaagt tagacaaaat tgacgaaaaa tacaggataa tgagaaaggg
      601 aggaggagag gagtgaaatg agacaagaaa gaagggaaag gtgaaagagg agcgagaaaa
      661 cagtagagga caaaggggaa agagttctgt gactgaaaag tataaacaca cattaggtaa
      721 ttgaactcag ctgtgtgctc acaaaagcag aaaacacaca cggcagccac tgtaaaaact
      781 taaaagaaca tttcttttct ttcacaccga ttcattgtct tcctttgtct gcttgtctgg
      841 gtcatgctct cctcaggtgc agaacttagc cactgtgaac aaagaggaaa tcaatcactg
      901 ttcaccaatc agctaagctc tgtgcctggc tggagagagg atacaaaacc tgtctggagg
      961 actgacgtgt acacacactc tcacacaaaa cacttcccat caagacccct tggctccctc
     1021 atcatccctt ttttcttctg agactcatca actactcaaa cacaatcaaa cgcactctta
     1081 gataatcatg tcttctttgg ttttaagttt ctggggggac aaatgaaagt ttgtgtgtgt
     1141 gtgtgtgtgt gtgt
//
LOCUS       KCVB01000229            1458 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10744pri ultra-conserved element locus
            uce-746, sequence.
ACCESSION   KCVB01000229
VERSION     KCVB01000229.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10744pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1458
                     /note="ultra-conserved element locus uce-746"
BASE COUNT          490 a          332 c          280 g          356 t
ORIGIN      
        1 ctgatggaat acaaacactt gttttgaaag aagctttaga tcttaatgct agttcacagt
       61 atccttattc cccagttaga aaataaaaag tagtcatgct ctatttttcc tgaaaggact
      121 ataagacgag taaagaacca aggaaattca gcttctgtcg gaaacaaata tgcataataa
      181 tgtgcaacaa agttcaaagg cggaacaaaa atgtctccac aaataaatta attgtttgag
      241 gatatcatgt gtaagcgtag attatatcat tgtaacccgt gcgcttattc tgggctgatg
      301 ctaaatgagt tatctaacct tgaatacaaa gagtcatttc acatgggtgc tttcagctga
      361 gattcaaacc atctgcactt gaaatgcaat gcaaaacttt tacaaaggta gcggagggga
      421 aaaaggttgg ggacaaaaag tagagaaaaa aaagaaaaca gggaaagaag gagaaaaaaa
      481 gtaaactgtg tatctaatac acacacaaac ttagacacgt agacaagacg acacataaat
      541 gtacacgcac acatgcacac accctgtgtc cattagccgg agccactgat acacagcata
      601 atggacatgg ctgatgtgct ggtgaatatg ggatgatgac acagatattc ctctttgtag
      661 cgctgacctg ctgcagtgaa aggctgccaa gcgtgatcat gtcaggccgc acacgctccc
      721 tcagctgctg ggcgtagtgt gggatcactt ccttatctgt cctcactgca aaaaataata
      781 cagtggcatc aaaccttaga ctatttatca aaaataaaat gttaccgtgg catcatgtat
      841 aactcttttg ctccaccgat aaaatctgtg ggagtaaaat attgtccgga cagaaagaat
      901 ttcacaaaaa tatattcaac ttcctaaaat gagtccttgc tagcattaat gattgatttc
      961 atattatgtt cttgcaaaaa aaaaaatcat tttacatgat cattctatac ttaatagtgt
     1021 aaaaatataa tatactaaca taatgactat ctgcataaga aatcaaatac aaaaacaaat
     1081 aaaagcatag cattactttt tacagtgctc tctctgccca ttaccatcct ccctgcttcg
     1141 agtccagtct ctcctccctg cccagcacta gccgcagcct gggttttggc aatggtatgg
     1201 gcctgggagg gcccggagcc cgaggcatca ccaccaccgt ggagcagcag tcgccgctga
     1261 agcaccggat cacccgcctt cacctccaac acctcctcat ccctggaggc tagcagcgta
     1321 cctggagagt tacgcatcag taggcaagca cagacgcaca cacacccaga cacacacaca
     1381 cacacacaca cacacacaca cacacacacc tacctgtctg tctgtggcta gaaacaaatt
     1441 ttggtggcgt cagacaat
//
LOCUS       KCVB01000230            1569 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10790pri ultra-conserved element locus
            uce-447, sequence.
ACCESSION   KCVB01000230
VERSION     KCVB01000230.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10790pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1569
                     /note="ultra-conserved element locus uce-447"
BASE COUNT          450 a          342 c          339 g          438 t
ORIGIN      
        1 ccgtcaaagg ggagctgagc gctgagcata gctgtgctga tatagtaata aagagcacga
       61 ccgcccgtgt gtcatccccg cactccccac tcatctgtca accatgctcc gacggccgcc
      121 tctgcaaata tatgcaaaac agatggatac ggccccttca cacacttccc cccaatttcc
      181 gtctctttca cacactgcag gactccccac cctgaggtgc cctagacata cacacaaaca
      241 tatgcattta cacacatccg aacagcttca catctcaggt ttcccacttt ccccgtttca
      301 gagatgatct aaaaccaatt acactgattg aggcacaaag ccgtttaaaa tgattacagc
      361 aaaaagcatt gttctgcgtt taacaggaca agcaaagcaa actacaaaac aaacctggca
      421 ttgcacagcg cgagaaggat gaggcgagtg atgtagaggg aaaggaggtt tgggggggag
      481 gatgagaaaa gttttgtccc ctgatttatg cctctttccc ggctgggata tgaatctggg
      541 atgatgctcc agagagcagc actccagata atgttagccc atttaaaaag agcagatttg
      601 tgtagtctat cagggtcgca gagtattttt tttttcccct cctccccctt ctacggcccc
      661 tccgtagtga gaaattggtt gttatcatag atcacagata ctgctcaaac ctcttatgaa
      721 agatgaatta atttcactaa tgctttaatg caaagctctc atgagggagg cagactggtg
      781 ataacccctg cctcgtcccc gcttcctccg ctagtgattt atgtcctctt ttagtagatg
      841 aaaccgccta gatttgccat ccatctacag ctttgaggaa tcctattaca cggggatctt
      901 ggatcggaca gaacaccgca cagagtccca aaccagttgc ttgagtccca gctctcagtt
      961 ttgggtcatg tagccttaga tgtgcggact aattgtttaa acaaatgcgg aaagatacca
     1021 gaactgacta acttaaagtt aacaaaaaag gcacaggagg taggtatctc atttataatt
     1081 aaaaaattaa aaaaaaaatg tttatttgaa attcatccag tttctcagac agaatcctct
     1141 gaggaaggtt agagagtgat agcagtcagc tcaggtggct gatgggagac agatgtgtgt
     1201 ttctcttctt gtgtcttacc tttatggtcc cccattaata aagtgtcact gagtgagctg
     1261 ctgatgacat catggtggga gggctcggtg tttgtgtatg ttagtttgtg tgtgtaaaag
     1321 agaaacataa tgtcacacag tacattttac tgctcttgct tggtggttta gacgcatgaa
     1381 gtttgtaaat aatgcagagt gtgtaaagct tcatagggat ctgtatgcag agtggggctg
     1441 cagttaacaa ttactttaat tattgattaa tctgcaattt attttcgaga taaagtgatt
     1501 aatcattttg tctacagaaa cttaaaaaaa ctcacggtga tatggtcaaa ttgttccttt
     1561 tttccacaa
//
LOCUS       KCVB01000231             986 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10829pri ultra-conserved element locus
            uce-92, sequence.
ACCESSION   KCVB01000231
VERSION     KCVB01000231.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 986)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10829pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>986
                     /note="ultra-conserved element locus uce-92"
BASE COUNT          221 a          165 c          315 g          285 t
ORIGIN      
        1 agcgagggtg tgatatagac ctcagcctaa gagaaaagag cagcttgttt gtcaattaaa
       61 agacgtgata aagcatgaaa tgggctgcca acgccactcg ggacgaggga gtgagagagt
      121 gagggaagaa gagtgtgtgt ttgcatatat cttggcgtac actttgtgtg tgtgttgggg
      181 gaggtctgtc actctcctct atggagatta attagcaggg gctgtctaat gaaaaggcag
      241 catcgttagt gctagggtag cacggcggga gaggcggagg gaggaggagg acgacagaga
      301 agttcctggg gagacgctgg cagagaggat gggagttgag tttaattgtt gctcacatgg
      361 tggcgtggga tgctatatgt tttgctgtag agtttcactt tgagccactg aggaggaaaa
      421 gatgctgatg aaagagaaag atttcttggt tgtctcacaa gatgatgaaa ccaccttttt
      481 tttgtgtatt ccagcgtgtg tgtgttattg tgtgtgtgtg acggtgtgct ttaacgggtg
      541 gaggattgtg acccaggtct aattgaagta tttaagccat ttaagcacta ggaacagaag
      601 gtcgttacag atccaaactg gcatgggggt taggggcccg cacacacaca cacgcacaaa
      661 cacacctcca cacatgcata cacagggctc cttctgttta agacagcctc attttattca
      721 tgatgctgct tatgaccctg aactccccaa tgtgagaagg tttgcgtgtg tattagtgcg
      781 tgtgtgtgtg tgtatgagtg agtgtgtgtg tgtttgcttt gggagatgcg actctgtcac
      841 cttccgctgg gctcagccag gtgtgaaaag gcattctgtc agacctccct gctactgggt
      901 ccatttatcc ttcgtgtttg tgtgcgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      961 tgtgtgtgtg tgtgtgtgtg tgtgtg
//
LOCUS       KCVB01000232            1356 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10871pri ultra-conserved element locus
            uce-1200, sequence.
ACCESSION   KCVB01000232
VERSION     KCVB01000232.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10871pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1356
                     /note="ultra-conserved element locus uce-1200"
BASE COUNT          360 a          290 c          303 g          403 t
ORIGIN      
        1 attattgtgt ttttccatat gttccataca tgcaaggcca agattcattc agtctggaaa
       61 cctggaatac aaatcccaat aaagcagcgt tttatttaag gtcatttgtc gcagtctcct
      121 tggccattta tttttctaca gcaacagcat attttgcaga attacttttg gaagaattac
      181 atacttttct attcttctat aatgcacttt cggcacttgc acttctgtaa aagctcagta
      241 gacataaaat ggttctactt atttagtttt cagtctagct ttgcacctct ggacagatca
      301 ataaagtagt tttaagtagc tttagcgtgc taactctgag agaaactggg tgtgactact
      361 ccggtaaagg gtctacaggg ggagcagcac cggctgaaaa agtggagatc atagcagacc
      421 ttagccagaa cacgggggaa aagtcattgc tcagagcagg ttgatttcat gccccaatgc
      481 agcagcagta attgcaccca gacctgtttt attgcgagtt gattttaagg agggatgaat
      541 ggagaaacat tctgtaaagt gtttattttg ggggatgacc tttattaacc cattttccag
      601 gcaggttccc ctgaggtggc ctggctcagt gctagattaa ccctcgctgg cccatggtag
      661 ccgggctgct ccccatcata gagtttcagg aagcagagaa tgacagtaag attgggcctt
      721 gcaacatgcg tgcaaaaccc ccacacacca cttttgagac ataagctact accaaccctt
      781 gacacacaca tgcagggagt ttgttgctgg cttgcctact aggaatatcg aagcagagtg
      841 gatgctgaag gttaatttct aagtagaaca gacagagaat gacattaaaa tgaaatatag
      901 caagtccttt tcaacttgtt taattttttt tctctgctga aaacagttgt agtcaataag
      961 tggactgtct ttagcctttt atcatctgaa taaatgattg tacgagattg atctacctta
     1021 agttagtgtt catgcttgca cagttcacgc tgttcaattc agtctctgca cctgtgcaat
     1081 acattcacca ctcttcttgc tgacacgcta acatctatgc aacagaaaca gtgttggtga
     1141 atgcttcttt catcacgcca tatggacctt ctgagcctct ctgctctctg tcctgcatgt
     1201 tgttcctcaa actgatgctg gaacaatgtg cgtatgtggg gcctttgatg tacagaggtg
     1261 aaattgtggt ttggattaag agtgggcgca gctcggccag gtattgtgtg agcacacgag
     1321 ggccatcatt gccgcggagc cccctgtcag cacgcg
//
LOCUS       KCVB01000233            1331 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10916pri ultra-conserved element locus
            uce-232, sequence.
ACCESSION   KCVB01000233
VERSION     KCVB01000233.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10916pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1331
                     /note="ultra-conserved element locus uce-232"
BASE COUNT          375 a          294 c          274 g          388 t
ORIGIN      
        1 tactagaagc acggccagtt tgaagggtta gtcatttatt tgagcaacgt ctgaagaaag
       61 aggggcttgc atttgctgta aactacgcat taaaacttgc ctgtaagact aaaacatttg
      121 tgtatttgga gacagagcag atggagtttg gttaggttca tcagagcagt ttgcttcgag
      181 catatcaaat atagtttaca cacagaggaa tttcagagtc tagcaatgcc tgcaatgcat
      241 attcatctgc tgcgcacaga cgcacataat caaacagagg ggaagacaaa cagagagcat
      301 ggtctgtaaa tcacctgtgg gttacctctg ccttttgaat gactgtgaaa tcagctcctt
      361 ttctctctcc ttcccttgct tccttcctct tcaaatccac tttttctcct caaccatgct
      421 atcccccagt tcccttacag agatcacttt ctacactttc cttctttttt cctcaacctc
      481 cctgacgaga gcacagcacc tcctttcttt tggcggcgtg gagtcctctg ttaattttcc
      541 ttcctccttc cttctctttc ctccctcctg tgagattgct gttgtgtctt atggcttttt
      601 attcctacgt gatggtagat gggttcatgt aggagtagaa gccactaaac acgcggtgag
      661 tgctcaacgc gctggccaac acacgcagcg agcttcctgt ggatatgaca gctgccatgt
      721 tcacatcagt gtactgccca ttatcaagaa tgtgcacaca gtaaaaatag aacaggctta
      781 atcactgcag agagggggta atcttttagg aacagatctg taatgtgaaa aatcttttta
      841 aatgacttaa ttaaatggtt attatactta attgaacaat tggtttttag gataatagag
      901 cagaatgtgc cataagtttg ggctgacaaa tcaatctgag caaagttggg agcagaccta
      961 cttttctcaa agacttccca cctgcatgct ggctgcacgt gccctgaatt tcaaactcat
     1021 tcctgcattc ctctgccccc ggggtcggcg tacaaaagtg gagctgtaaa aatctcaaaa
     1081 tagagatcaa gaggagtggg tgcgcaattg aaattaaaca acatgccgag gtaggtcatc
     1141 agtggtgttt aataatgtag agaatctgga aacctgttac agaatactat acacatctta
     1201 acatgcagaa gtgtgaaaat aaagccactc ttagggggaa aagcacatgt gaatttggac
     1261 tgttcatatt tcagatattg cacagcacaa acatcagttt tacctgctat tgctgtccac
     1321 taaaaacaac a
//
LOCUS       KCVB01000234            1342 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 10959pri ultra-conserved element locus
            uce-277, sequence.
ACCESSION   KCVB01000234
VERSION     KCVB01000234.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="10959pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1342
                     /note="ultra-conserved element locus uce-277"
BASE COUNT          411 a          218 c          287 g          426 t
ORIGIN      
        1 ttttgtaagg cctcacaggc agaaaacagc ggcagtgttt tatagtaagt atatagaatc
       61 aggcatggtt gatgtgtgac aaataggaca ttagtttaga tctgtttaca tgaaatgcac
      121 aataacattg cagcagagca ttggggtgga acgacagaga cagcttggcc ttccatttat
      181 gagggataga aagatggatt ttaagggaga ttgtgagtaa aggcagtttg aatggaagca
      241 acaaaattga atcctctttt cttttatttg tttttgtgga atacactcgt ccaatctgtc
      301 caagttgtgt caaaatattt tttaacgtct ttttcttaag tctggagcct tattatatat
      361 gggcataaat tacatgcttt aaatttctgt gctgtatatg tttatatgtt tagggttggc
      421 atgtttaccc ttgtgcacgt tagcatgacg agaggaaaga gattcgtgac tgcattttga
      481 tgtcctgcta ccccgttctc cattttaaaa gctggtacat gtgagaatga gagcgggcga
      541 aacaggcgtg cagccctcct agcagacagc aaggaggatc acagggagga aagcaaagaa
      601 tttttatcat acagagaaat gcaacggaaa atacaagagc aagcagttat acaaagagac
      661 aaccgggaga gacagacagg caggaagaca gagagacagg ccgagacaaa tacacagtgc
      721 tcgtccctag tttggtaatc cgatggagag attagcaagc aggcagcatt tgggtcggat
      781 tatcgagtca ttagaaaaga ctggtgctcc agagcctctc tcatgtgtta atgtggcttt
      841 acagatcctc agtgcagcct gcgcagattg tctagattgt gttgagagta tatatagtgt
      901 atagtttttc aatatgtaaa tagggccaca tcaacctaat gcccccatgg attgattcct
      961 ttgtatatat gtgtattatt ttggtgccat atgaaataaa ccaattaata cagttttcct
     1021 tttttttatt gttttaattt ttatagttat tttcattatt ccccatttaa ccaacatgaa
     1081 ggaaaataat cgcagcaatt gtattttttt ttttccttct tcgggtgagg aagtctccca
     1141 gtgcaatgct gcagaagtgt ttattccaga attaaagtgt gtcacacata aatgtcccct
     1201 tcatgttggt gaaactaatt aatataaata aaatttttat ttttgcagaa gttaaatttc
     1261 caatttgtgt ttattccagg ggaaaaataa aaatttgaac tcaaaatttg caacttacca
     1321 cctatcatca aaatgtattt tt
//
LOCUS       KCVB01000235            1388 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11002pri ultra-conserved element locus
            uce-1144, sequence.
ACCESSION   KCVB01000235
VERSION     KCVB01000235.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11002pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1388
                     /note="ultra-conserved element locus uce-1144"
BASE COUNT          400 a          313 c          253 g          422 t
ORIGIN      
        1 attattatta ttaataataa taataataat ttttgttatt attggaatta tattgttttt
       61 gttatggccc tcattatcag tatcacatta ggatgggcct aatgtcttaa atacttgctt
      121 agcgttttca aacaaacaga gtcctgcttc agtttcaaca cagcctcttg tccttaagcc
      181 ctggcaaaaa tattgacaca aaaggtttac ataaattatc cccgaatatt tgaacaatag
      241 ccgctcttcc tcgtggttcc catcgctaaa aataaagaat attttgtcta cggaacccca
      301 aaatgtgggt ttttaaaaaa aatgtacgaa tatttttgtg aggaaagtga aaaaagacga
      361 aagcttcagt gggagcaatt tgttaaaacg ggcttttaaa gtattgtgag tataaagcta
      421 cttggaggtt ctttgtatgt aaatagggtg taaaaaaggc cctccccgtc tttgtaatta
      481 attgacacgt tataccactc atcatgctct gaagcaccaa tggtagaggc tcggatctcc
      541 ccaaaaccca caatttcaca tctgcaaaca ctgtcttcat ccacttgact cctaagaccc
      601 gcccacacgt ggccaacctt tcgcgttttt taatgctttt tcactgcagg ttcatgtgtt
      661 gagaccaacc ttatatggac tgagcggaca aggtaatggc ttatttccct ctagcaccca
      721 gcagtagcac agtatccatg agccacatat agcacttcag ccactttggc attcttcctg
      781 gacttacaga caccgaatcc actgccactt gcttaggcaa tggttatcca cagtggctgc
      841 tttgactgta cttcagcgta gagcgactct tactcatcct atttttcctg aaacgcctgt
      901 gcatcttctt cttcttcttt tcttcttctt tttgtttcaa aacagcggat tgtccgaaaa
      961 agctggtgca gcaacttttc cctgcatatt cccccccacc agaatatgtc gttgaccaac
     1021 acaaagacgg gcttttctgt aaaggacatt ttggaccttc ctgacacaaa tgacgaagaa
     1081 ggatctatca ccggagcgga ggaagacaca gagggatcgg agaccacgtc cacgacaaaa
     1141 aacacgggag ttttggtgca aagtcctcta gaaaacgttc aaaatctgcc tttaaagaac
     1201 cccttttatg acagtagtga caatccttac acacgatggc ttgctactac ggacagtatt
     1261 caatattcat gtaagtagag tctaagaatc cttgttgatc ttgttgataa tgacttctgt
     1321 gataatttta agctatgcgg ttacattaag ccataattga tggcatgcag cctcctattc
     1381 caccctat
//
LOCUS       KCVB01000236            1612 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11044pri ultra-conserved element locus
            uce-1101, sequence.
ACCESSION   KCVB01000236
VERSION     KCVB01000236.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11044pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1612
                     /note="ultra-conserved element locus uce-1101"
BASE COUNT          448 a          382 c          323 g          459 t
ORIGIN      
        1 gatgcattcc tttgaatatg caagaaataa tcatcactca ttccaacagc ctgcatagca
       61 ggaccctgaa ggtttttacc tcaaagcgat ggaaataatt tcctagctct gaatggcaga
      121 cattttttct ctttgcactg tgaaactaaa tgtaaggctc atttgcctgc attgaacagc
      181 tcattttatg catgctgaaa agcagaggca aatggacaag gacaaaaatt gcatggtcat
      241 tgaaatctct tttcaaaaca atggctagtg tcctttaaat gaagttgttc tgtctagcca
      301 ataacacgaa gcagattttc acccgtggct taaactccac gaggtcttca agcatgggcg
      361 aggaagcaaa ggttaaatca tgcaccacaa ctgtcctccg acgtctatat gctctaaatt
      421 taactccttt aggctttata aaaattcatg ctgtcggtgc aaaaacaaca gctcctacaa
      481 gacgcaactc ccccggcttt ctcaatcttc tccaagcctc tgtaaggcaa gaaggacgat
      541 ccccatttgg agctcattgg agcaaatcag aattgtcagc actgtgggtt tttacctttt
      601 taaacgggga agatggatag ataatcacca catgccattc tgggggccaa ttacacagct
      661 ttgtgtgaaa ttggaaaacc tgttctctcg gagctctaat ggatgttctg gtcattagtt
      721 atttgagagt cttctctggc tgtaatcaaa cgtgaaacat tgcgttaagc gcccataaaa
      781 gaacagctgc ctctctaagt gaccttctgt ttaagagaac ccaactgtca gccgtgtggc
      841 gttttgcgac aattattatg tgtgtcgcca taaaagcagc tcttattttc catctttttg
      901 cagtggttgc atctcatttt aatgtcttgc tcttggttgt atttgctttt ctgggtctat
      961 taggtggctg tcatgtctta catacaaggc agatgcataa tattatccat ctgctgtctc
     1021 cttactctga attttaatcg gcaaaacaaa ctacttcagc tcatgatatt acaactgttg
     1081 ctactataac acacaaacta atattttgat gtctttttcc ctctgcttcg cttcaggtga
     1141 ccatctatct agtcagcaag gcccacctcc tctaccacca gccccacccc cacacaagca
     1201 gcatccgtcc atcacgtccc tgagccgcag ctcactggcc aatcagagga gcccaagccc
     1261 gccgcccaca gccggcctgg cggccgactt gcagagtacg gcggaatgcg tccagctgca
     1321 ggacagctgg gtcctgggca gcaatgtggc cctggagagc aggtgagtga cccgcaatga
     1381 cctaaaccag agctggaaaa ttcaggtaca ggtgttatta taaagccaat tccactggct
     1441 cagaaaacaa aatgatgtga aacaaaatca acaggggcca agctgctgtt ttaaaatgtt
     1501 tatttttaat ttctgttcca gtacaatgtg gcagctcttt aaatttgttc caagacctac
     1561 cctatggtta aattttatac attttacatt tgagaatcat gctaagaaag ag
//
LOCUS       KCVB01000237            1509 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11085pri ultra-conserved element locus
            uce-133, sequence.
ACCESSION   KCVB01000237
VERSION     KCVB01000237.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11085pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1509
                     /note="ultra-conserved element locus uce-133"
BASE COUNT          406 a          356 c          303 g          444 t
ORIGIN      
        1 cagaactaaa tgacagtttg attacataga ttatcttgag attttgaact tctactttct
       61 ccactgggtc tcttattgat caattataaa tcattgcctt tgagctgaca atgtaaaacc
      121 agtttgatag acaaacaagg aaacacctgt gacatacagc cctcagtcaa acgactcact
      181 cgtgagtaac attttcacac ccaatggtaa gtctgagtga caacatcctg ttcctctctg
      241 cttgtcaaca ggcctgaagc aacgatagta taagcgtgtg tgttcattag aatagtacct
      301 gtctgggact cccttagcca cctggctttt caaacagaag caactaacat tcctaatctt
      361 tttttctata tgccacaggc agacaagatc caaatccgca ttgaaacaac aaaaccaaac
      421 atcacagcgt tcccaaatgt cacattattg tgtatcgtac acggacagaa gctaacgaga
      481 cggggagaca ggaacgtgat tctccttgga gagagacaat ttttcgctgc cgcgtccgag
      541 tccaaaaata acaaagaaca aaggcggata aatgagtgag tttttgagca caggctagtg
      601 ggctttggca gcaccggcca ggcaggaggg gagggagaga cagggagagc tagctagcgg
      661 cccgtccaca catctgtcca cacagcacgg ggtgggttcc agcgacaccc acagaaacag
      721 gcttaacctt ctcttccccc caccacctgt tcattactat gggccccccc accgagccac
      781 cgccactctg ctctcctcct ctcacccgct cccttgttct tgttctctct ctgtctgtcg
      841 ctactcgttc cagttctctc tttgttatcc tgccgttttt tttttttttt tactcaatct
      901 ctccctcttt cttttatttt tttgcttatt tatttctcag cagtgaagcg ttatctctct
      961 ttctcactgt ctcttcccga tgaggctttt agttgaagtt aaaaatctct gcgactaata
     1021 ggaagatagt cttgctgtaa caggaaggga tcatcagaca ggttgtttta accagtgcga
     1081 aggagaaaga agaggccggc ttacgtggag ggggagaaga agaggctttt gatgaattgt
     1141 ctgtgaaagt gcatcagtct acccacatct tcaaaccccc tggtgcctct tgtctgcttt
     1201 cagacccatt tttctccaca ttgttggtaa tttgagcatt cttgcccatg gcatcattca
     1261 tagttggccc atttaaatcc ctttagaaat tcaatttgga gaaaggaaat gacaagattt
     1321 agcttattga ggtatgccag aatatccatg aaaatcagac atgggaatat gtgagattgt
     1381 aaaagcctca tcatcgctcc ccttttgtta ccatgcgaaa aaggcgctga atattctctg
     1441 tacttgttta gggttgttta aatttttgat cattaatatt agcaaaggtg aatcataaaa
     1501 tagccttta
//
LOCUS       KCVB01000238            1264 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11121pri ultra-conserved element locus
            uce-970, sequence.
ACCESSION   KCVB01000238
VERSION     KCVB01000238.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11121pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1264
                     /note="ultra-conserved element locus uce-970"
BASE COUNT          392 a          211 c          279 g          382 t
ORIGIN      
        1 ttcttcctct tgtccttatc ctcctcctcc ataggacaga aatagatggg cagattccca
       61 tgcagggtgg ctaagatgta ctttcactgc attttctcct tgtgctacat ttataatgct
      121 aatcaaaaat ttatagaaaa ctccacatca tttaaatgtg agctgtgcat atttttcttt
      181 tggatcaact aggacatgcg tatgactact tcttaacatc agcctgtgtg tgagtatgtg
      241 tgtgtgtgtg tgtgtgtgtg gggggggggg ggttctaata gtattgggta ttagaaaggc
      301 tgcttattat tgaatagagt ctctgtgagg tttccgaggc aggaggacat ctattaggag
      361 gttagcaaaa cactatcagc taatacgacc tgtaattaca ccagcagtga ggaaacacag
      421 caagctaaca ccagtatgga aacaatctga caatgcaaac actgcaacta agccactacg
      481 cgtgtgtatg tacatttgta agcgtgtata tagttaagtg agtgcacgta tatgtgtgtg
      541 tggggacgga ggcctccttg caaagattgt gggtgtgcga actgtcaaat tggagtgggt
      601 gggtgggagt ggagggagca catctggagg gaatttattg tataattcag tctattattg
      661 ctgcggtcgg tgcttaagga gcacgcacac aattaccgtc cactctgcaa ggctacaagt
      721 ggcctctggc ctaaaccaat aaactgtgga ggagggtgga gagaggagaa ttcatttgag
      781 gttatcatca tcacactcct ctctgctacc tctcacactg acacttagca gagtttacaa
      841 taaatcaatg aggaacacct actgtttagg cccagattat ggtcaaatta gatttgaatc
      901 tggctttaat gtttgaatta gacacgcatt gctagaacag cacatgccac gcgaggtttg
      961 ttcaactaca ttgtttggaa gcacagaaaa taaaaataaa ttttcacaaa aaaaaacttt
     1021 tatgtcattt taattagatt tttctgttta ttttttaatg taaaacgtaa aacatttaaa
     1081 atatttgtat gtaaaatatg cagtggtgaa agaagcaaat aacagtggat aaaaatactc
     1141 ctcttaaaac ataaattctg cattcaagat cctgcttaaa agtattgtag tatttatagc
     1201 aaattgtttt agtgaacaaa tatcaaaaga acttatttaa aaagaaaggc aatatgatag
     1261 tgat
//
LOCUS       KCVB01000239            1445 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11163pri ultra-conserved element locus
            uce-176, sequence.
ACCESSION   KCVB01000239
VERSION     KCVB01000239.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11163pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1445
                     /note="ultra-conserved element locus uce-176"
BASE COUNT          359 a          330 c          335 g          421 t
ORIGIN      
        1 gtgccaaact aatcggatac ttgtggtcag ggcagcatct gtgcttttaa tatgatctat
       61 gaatgtattg tagctgcagc cttgttagct gtgagctgtt agaggcagaa cgtgaaaaat
      121 gttgatttga agccacagca agggaatgca aagataaagc ggggaggaaa gcagaggaga
      181 gagctcagtc acacacaccc tgagagccgg agaactaatc gcggtcatcg ataatgtagc
      241 gtgtcagccg gcttcctgca gtggcttttg cacatgcccc tttctcttta aaacacacac
      301 actaacacac agcaggctgc ggccggtgaa gtcctcctct gcccccgacg ctgcacactg
      361 ctcgtccagc cagccgggct gagggtcgtg gtgtggctgg ccgggcgcca ggacgtgtgc
      421 agggggctct gccatggcgt tggccctcgc aggctggcag cattgaggag cgtaaaggca
      481 ttcagtagcg tgaatgtccc tttgtctcag agcagcgtgc caggctagct ctaatgaggc
      541 aactctttgt ggcaggcctc tcaacatcaa gtgggctggt ggactcatta acctgactac
      601 tgcaaataaa gcaggcaggc acaagagacg cctatgatag cacacttaag gcccagctga
      661 atccccccac cgccgccatc atccctccac ctcctccctc tcctcttttc cctccagtct
      721 ctcaggcttc ccagggctcc tctgatagga ggaaatcagc ctctggccta tttcaggtgt
      781 cccaaataag ggatgggggg tgatttccac tttccttatt ctgtctcctt cataccttga
      841 gctttaatca gtttttggct ctgcactgta acctggtttt tgctgaaaaa tgaatgaact
      901 gggtttgctt gttctctggg acagatgtag tctgttggtg caagtgttgt tatatgttaa
      961 tgcagtccac tatttctttc tgtagattta tctcaatgaa ctgcttgaag ctatctctgt
     1021 cgtggtaaag aacacatacc tcttactgcc tcataaatat aatgtgcgaa ctgtgaatag
     1081 atttaatata atgtaaaaca ctcttggtgt gcagacttta ttggatgttg ggcccaaaaa
     1141 cactgaatct gatgctttag atgtttaagc acatgtgact atatgcatct atattatagt
     1201 cacattgtcc ttccaactat ctatggttct ttgttgttct ggattaacat ttaagcacag
     1261 ttttatcagt aacagcataa cagacacaca gactaactgc ggtgtattct gtgttttgtt
     1321 ttattcaaaa gaggaaaagt tcaaaggaga tgtgagtgat gtgtggtaga aaatatttaa
     1381 atcatcctct cttttaatta ttagacgatt tttaccatgt ccaatttatt tttctttacc
     1441 attta
//
LOCUS       KCVB01000240            1111 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11206pri ultra-conserved element locus
            uce-935, sequence.
ACCESSION   KCVB01000240
VERSION     KCVB01000240.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11206pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1111
                     /note="ultra-conserved element locus uce-935"
BASE COUNT          280 a          231 c          206 g          394 t
ORIGIN      
        1 tttggggata agcaattgtt ttctcattga gcgacatttg aaggtcaact cttcgccgag
       61 cagggcactt gtgagaagcc aaagaatttc atttcagtat gaataatgca ttaaatacag
      121 ttatacaagt gtcttttctc ataataatga ccgaagtttt tgatctcata ttctagttct
      181 atgttatcca ctagcatgct tttgggtaat gtatattcct aagtacagtg tagaatatga
      241 gagctcatta ttcctttttt tttttacatg attccttttc tgctatatat ttaaatgcac
      301 atctcatttt gctgttgctg aaatgtttga tcaaatctgt cacttccttt tttatcatgt
      361 aatgaacatt gttccttaac actggccaga aatactgtgc tctacaatct gcacttttca
      421 acattttgtt ctgtttcttg ttgccatgct attgttgctc atgtatgtat gagagcgagg
      481 aaattctaaa cccaaatgtg gcgttgtctg tttctttttc ttgttctaca gagatgttga
      541 aggagctgaa ccaacagcgc agagcgaaag agtttacaga cctgaaaatt attgttgaag
      601 gcaaagagtt tgaagtccac caaaatgttc tagcttcctg cagcttgtat ttcaaggacc
      661 tggtgaaaag gtttgccttc gcttcccctc ccaccagctg ttgatcgaat ctgttctttt
      721 catagggcgc atttgtgtcg cttctttttt ttttttccct tcccctctct ctcttgcagg
      781 tgtgtgaaga ctttggttcc tagcacaggg ccagcagcca ttccttcctc ttcccgctca
      841 aatgaatgta tctgaaactg gagaaaaaag gtcaaccctc gtcattacct ttcctcccca
      901 tgtagggcaa tttaaccaca tttccatttt aatgagcagt gaaaatgttc catgactggg
      961 gatgttttaa aactcaccaa agggccaaaa caaaagaatc atacattctg cactttgggt
     1021 ggtaattcac caaagccgca ctgtcttctg tgggcagagg gatttttgtc tcccaagcta
     1081 ctatattttt ctgttgctaa ttcttgactt t
//
LOCUS       KCVB01000241            1187 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11243pri ultra-conserved element locus
            uce-623, sequence.
ACCESSION   KCVB01000241
VERSION     KCVB01000241.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11243pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1187
                     /note="ultra-conserved element locus uce-623"
BASE COUNT          458 a          226 c          212 g          291 t
ORIGIN      
        1 taatatgaaa caagctagaa ttaaaacatg catgcaaact gaacgctttc ataacaccgg
       61 ataactcttg atgaccaacc atccttatcc aatctctcct cctaaatatt tggccaagtc
      121 tgatgaagct ctaaaaaccg aggcagcccc catgtatgtc catcgtcact tttttgtcaa
      181 ctgcctatca gagtgttttt aactgtgtag atgtcaagta tacaaagata aggagacata
      241 caggcaaaag aacatgtata agccaatgtc acataagact gttaagctac aaagtataaa
      301 tgaaacccct taagacatac aggcgaagat ggaaataatg acggcatgaa agaaaaggac
      361 gatgacgaat ccaagacaga aagcctccag ctcattaagc acagacagcg tgaatgaaac
      421 agcatgcgca gaaaacaaca aaaagaacag aaagaacaga gagaaatttt ccttataaaa
      481 gtttaaaaaa aaaggctgaa atgaccacat gtgtggcatc aggagaaaga gacacagagt
      541 ggcagattaa gatgaggaga gactgaaagt gaccggccta cctaatacag gcactattgc
      601 atagcatgag tccaaaaact cttgagtagg gataccattg tcgtcgtcca gtcttatatc
      661 actaaatcta aaaaaagtag aacagattca aacaggagaa tgtattagag tcaagtatgc
      721 catttcattt tttatttgat caaatcaaac agaaaaaacg aggcaggtac aaaggtcaaa
      781 acaaagccag agagatgaat cacacaatgt catgcaattc tacgcctgac aatacctgag
      841 accattctgc tactttgtct atgactttac agagttgttt ctttttattt tttatattaa
      901 tgttctaaaa ctgtcccatt aatttaaaga ccaactccaa aatgtgcata ctgtagtcac
      961 catcttgcag ttgaatactg cataagtgag acagtgataa actgcattat aatagtttgt
     1021 cataactgaa taatacagaa acggttataa acactctagt ccattgttca gaaaaactct
     1081 acaacgtctt taaaaaagaa agtaaaacac atttgaaata cagagtgaaa cagaaaaagc
     1141 tttacaaacc caaacatcag aagatggcct ctctgtgtca tgtggtc
//
LOCUS       KCVB01000242            1279 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11282pri ultra-conserved element locus
            uce-666, sequence.
ACCESSION   KCVB01000242
VERSION     KCVB01000242.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11282pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1279
                     /note="ultra-conserved element locus uce-666"
BASE COUNT          380 a          252 c          266 g          381 t
ORIGIN      
        1 ttttattgca ttatactgac aggcgttact attatatcct ccaccttatt tatattaatg
       61 agagtaggtt aaataaaaga aacacctttt tgtataatgt ttttgtgaaa cagcagcaca
      121 aactacatcc tccaaaatga ttataaagtt gaatcaatcc ctcattacaa cagtttcaac
      181 aacatctaca taagggtaca atgtattgca gaattgttat gttagactgc attagtcgta
      241 attctagtaa tttctaaatg aaaaatttgc taaaatatat aaatctccta cacatatttt
      301 ggctgtgtaa atctagtgag atcctatttt actgtcagtg ttgttcaacc tgtactgaaa
      361 ctctgtagct gccccatctc tatctccatc tccagccagt ggcatctgtt ccaacctgcc
      421 aggcaagagg gagggaggga gggagggaga gagagagaga gagagagaga acttcatttc
      481 tagcaaatta atttcccatc cacgccccca gagcttgcta gctcgttcac tagctgctgc
      541 ggccgctgct gcgtgtgtct actgagtcgg agcctcatgc ataaggcatc aggtttaatt
      601 gcaaacccag aaactaatga ctttggactg gttaacagca ggggagccat acatcttgag
      661 caaactgaat gaaaaatcag ctcagaggag agaaaaaaag gagcctagcc cctaattgat
      721 gaataattga agcggcgagc tacagggcat aattgtgaca ttttgtttca atcaatttcc
      781 aagtggtgtg ggcgaaagtg cacttaaagg gaaaaggcaa tgacaaagaa tgaggaagaa
      841 agagagagat agagagagtg cttggtgatg gaaatggaca ttgggtcttc agtcattgca
      901 atgacatttg ctggggcaaa agaccagact cattcaaaca caaaatgact tttctctcta
      961 tcttcatctc actttgtagg ttttctgtct aactcctgtt atgctactgt gaggtcactt
     1021 tttcactccc cgccgatgga tctatacgct tttaaaatga tttctttagt ttcttttcac
     1081 ttgccttatt cgttttttaa attcattgca aagctactgt ccatttacgg cccagaaaaa
     1141 ctaaagtgag gctgccatct gtttaagtgg ttgtgtttgt gttttcagaa aatcctgaat
     1201 tccttttcca gtcagcttag ttactgtcag ggcattgggc caggtcaacc agtgtgttgg
     1261 aactgaacac cacacaagt
//
LOCUS       KCVB01000243            1250 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11327pri ultra-conserved element locus
            uce-1179, sequence.
ACCESSION   KCVB01000243
VERSION     KCVB01000243.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11327pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1250
                     /note="ultra-conserved element locus uce-1179"
BASE COUNT          280 a          288 c          324 g          358 t
ORIGIN      
        1 cagtcagtca gtgggtaatg tgtgttttat gctcctggct gaatgtggtg agttgtttgc
       61 ggaggctgct gagcaacacg gtgcctgaga ggcagggtgg aagaggagca ggtcacaaca
      121 ctgggccaca gcgctgttgc aggcattctc caatgatgtt gttgttattg ttgactttac
      181 ttcaaacagc tgctgaatcc catacatatt tcaaaagagt cttcctttaa tcctgccagt
      241 ccttctgcaa ttagccattg cagtgcctgc agttcatgat ctgatagaag aaggggaaac
      301 aaactagagg tcctttgatt ctcagctact gagcgtctcc tgttctcagc ctgctcattg
      361 ggacgcatct caaggagagg tggagacact tgagtgaatt ccccctcttt tgaagccgcc
      421 gacctaggtc aacctgtaat tctggcagct ggccagctcc tcgccttccc ctcatctccc
      481 tctctctccc ctcacgattt ccaaactgtg cttccctgag tgcaggtcgt gtcttttgtt
      541 acctttatga ggcgtaatta aatgtgcacg gcgtggcaca gtgtttgagc aggaacccca
      601 ggcgtgccat tagccagatg tggctgctca atccttaatg atagaggaac agaggcggag
      661 gagacgagcc caggagtcct tcatcacgcc atcccgaggt gtcgtgtggg cctaattgcc
      721 ccatcgtttt gtgcaccgta cgagccccca tgacacctag cttcatgcat tcattatgta
      781 gtctcccagt gctacttcag gctgtggtgc tcatgtataa ccggaggtgg gtggtgcact
      841 gcagctaggc ttttagccag caagtatgat ttataagggg agttcttaac acctttgttt
      901 tgtacattgc acgcaagaag tggggtgatg tactgtgtaa tgcagtgcat taacagttta
      961 cagtggctga ctggagaggg tgtcatcgtc atgtactgtc atcatgtgag actgtgagag
     1021 ggataaaatt tctatgtgca gctgctgtta aacatcacca aagatgcaca tcattatttg
     1081 tattcagttt tggctggatc attaagtgta gaggctgttg agttctgtca gcccgtgttc
     1141 taatagttga aaattgatgt atatgtccct ggaagagggg ccaacctcaa gcgttaagaa
     1201 gccacttttc tggaaaacaa cctgcttgtc tgtctctgcc tgggggcttt
//
LOCUS       KCVB01000244            1545 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11367pri ultra-conserved element locus
            uce-1285, sequence.
ACCESSION   KCVB01000244
VERSION     KCVB01000244.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11367pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1545
                     /note="ultra-conserved element locus uce-1285"
BASE COUNT          412 a          354 c          261 g          518 t
ORIGIN      
        1 gagatgacag tccaaggacg cttgggagga aacaaggtat accaggcatt cattagcttt
       61 ggcatggcag tcatacatta gcccataacc ctttattgtc ctaattaggc tggagagggg
      121 ctgttagcat taatactacc aatattatac ataaaatata catatatata tgtatgtaca
      181 tatttgtatg atgcctatat gtatgtaaat atatttgcat gcctatatgt atgtatacaa
      241 tatatcttta tgtgtatata tatatatata gagagagaga gatatataga tatagatata
      301 gatatataca cacacacaaa cacacatgaa agagttttta tgtcctgcac tttattatca
      361 ttttattttc tttgcctttt ctgcccaggt agcagtttgt ccatacagtg tattatgtta
      421 gctcacatcc agcccctaag ctgggcagtg tttttgattt acaatcactt gctcgggaca
      481 aacataagac aggcttcccc ttcctccatc attttactca tctcacttca cactgtgagg
      541 tgcaacccat actgtatatt tcatttcttt ttttcccctc cctcccctgt tttaagtctc
      601 cctggatgga gcctggcttg cggtgcacca ttttcccagt tacagtagca gtggtgctgg
      661 gccctgttca accccattat ttctgggagc caaattccca tggtcccagg aacaggcgtg
      721 ccagctctgc cttcttggct ctcttccctg gtattgtttc ctttgttaca tccagagctg
      781 catttcagcc cgttaatgtt tgcgctggga ggtccaaatt cccagtgttc agctttagaa
      841 aaggtaactt caagttcaga atgcaaccac tttttttttt cttctgcaaa ttatgttttt
      901 ttctctttta tttcttccat ttttgttctt tgttgtgata tactggagta tataccctga
      961 taacaacacc ataaacttgt tcaagacaaa atccttgtat gcggagcaat cataaaatcc
     1021 ctccccctac aaatggttta gccgaatgtc ttatctgtat ttttttttat tactttagag
     1081 caattaaaac attctttaac cacgtatgga atttacctct ccaaagaaaa ggtgatttca
     1141 cagtttataa attaatgtac acttaaactt tacacataag gatatgtgaa atatctccac
     1201 ctctaattca cacaacatga cacaatctcc agattatacc agggtctcca ccaaatagcg
     1261 attcactatt taacattttg agtctgactt actattcacg catgatggca tttccccatc
     1321 tgtaatccac tttatcaccc atcattccag ccttagcacc ctaactcctt ttctctgatg
     1381 tcaactcatg tagccatcat ggctgctcac acatgggatt aaatgcaggg tttgttggct
     1441 cagcgcagca tggagggagc atttccgact gatgttggct gttccactcc gagccacagc
     1501 acttgtctcc cagagcgcag acacgacggt gtcaggtaat tctct
//
LOCUS       KCVB01000245            1228 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11414pri ultra-conserved element locus
            uce-908, sequence.
ACCESSION   KCVB01000245
VERSION     KCVB01000245.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11414pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1228
                     /note="ultra-conserved element locus uce-908"
BASE COUNT          393 a          269 c          204 g          362 t
ORIGIN      
        1 acacacacac tcccacacac acttacacac tgccccagcc acccaacctg caaagccccc
       61 caggccactt cccaatgctg tttatggtgc ttcctccatt atctgacaac aaaaggagtc
      121 ctcaacacac aaaaccccag ccagctgtgt gcctgacatg gtggggaata cacagcccct
      181 ctccagcgac aataaaaaga aacttgagtt taaacaaaaa aagttacacc atatttgctc
      241 agactaacta tgatgaaagg caatgaagac aagggctcct catgtaggta tcaatataaa
      301 tattactgga aggtcaatta atatgtaaat taactcctcc atttaattag ctatttaact
      361 attttccaag gcatggacat attaaatcat gcttaaagac ccctctaact agcctcctga
      421 caatagtgcc agggagtcgt gggctcataa actaccggct tgacctataa agagccccga
      481 gtctgctaat tccatctcag cctaatttta tgacttgcat aaaataacca tagtgcagct
      541 ttgtagctgt aaagaggata tgggtaataa aacggagctg gggaaagcat cagaaatctc
      601 ctcttcacta gtgtcctcat ttattattct attattcttt gcaggctacc gggctcatgt
      661 gaaagacaaa tgaccttatt atattaaata atttgtgagg atattaaccg ttaatcacac
      721 ataaacagca tttaaaaggt attgattttc cagagctctc aacagcttgt taaatttacg
      781 tccaataaca tgataataga taatcaggtg aatgatactc atttgtaggg gacaaaggaa
      841 aggtaatggg catcactgac cctggctcca ccatatctct ctatcccact ctgtcatgac
      901 cttcttacag gtctttgacc ttgagcactt tctcagtact tgtaagtcca caatgattga
      961 cagccagaca ttagacatta tgtccagcca gggaatttag tttcaggttg tatcttttaa
     1021 aactacctag caaaactacc ttaacctgca aagcttgttc caaaacttca ttgtgaaagt
     1081 gtctttattt cagtgtgata tttgttattt taactgtgtt atttgttatt ttaattgtgc
     1141 taaaatctaa aaacttagac tgaaaaaact tttctaatgt gaattcaaag cagttgtttt
     1201 agtgcttaaa tgattagcca agtaatct
//
LOCUS       KCVB01000246            1445 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11493pri ultra-conserved element locus
            uce-567, sequence.
ACCESSION   KCVB01000246
VERSION     KCVB01000246.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11493pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1445
                     /note="ultra-conserved element locus uce-567"
BASE COUNT          455 a          281 c          334 g          375 t
ORIGIN      
        1 ctctaatgca cggctcatag gtatcaacat attcaaagct accaaactga taaaaaaaag
       61 acatgatatc tttatttaaa agctgtgcag ctgagggttg aactcccatg ttcctagatt
      121 gagaaacatt gaggagatca ttaaaaaaga gctctcacat gggaattaac aggagaaaaa
      181 gatacttcag aaaccagaga ttcctcttac tttgctcttt actctcctgc agcacctgac
      241 aggtgaggag cacctgcaaa acactcaaag caaccagctc accttgctcg ctaatcgagg
      301 gtctgtgggg gcgtcttcat cgacactgac ggctaactga cgtgagaaat aaaaagcagg
      361 aagtctgctg cagcaaggag actgaacctg actggctgct gctgctgctg ctgcgagggg
      421 acgagaacag aggattgtgg gggttgtatg acagcaacac tggggagcga aacgcagttc
      481 aggggccttt ttccaagagt ggtaatcagg cagaggggga tgaaaaaggc tgattttcag
      541 ggaattagca tgataagcga gaacacaaaa tcaatcctcg tcctcagccg aggcctcctc
      601 tgattacttt gaactgtcaa aaaatggcag aatcaaatcg aaagtgctcg aggttagggg
      661 agtgatttgt tcaagaagag atcgctagag caattgacac agctcttgag acaataattg
      721 ggttatcaaa gagaaaacag ttcaagtacc acgggggtga aagcttattt gcattggatt
      781 ttcatacgga gagacacacc tcttcttcac tgccgctgtt ttttcccccc tctttatact
      841 tgaggggcaa gttgacacgg ggattgcatc attatcatga aaaagtttgt tgccatgtgc
      901 gcgccgcatg attagcaagt ggtttgtgtg tatagagaca cacaaagtgg aattcactac
      961 agtatgtgtg tgtttgtgga agtgtgagtt tattatactt cctgggaata cgaagttctg
     1021 cagaggaata ttcacaaaca gaaagagttt ttcttcaaag ccaaaagttt actctttaaa
     1081 tcaactgtct ttttgctttt gttcagctgc aaaactcaac gtcctgctga gtttatggaa
     1141 acagatgcag cagagaagat ggaatatgat ccgaagattc ccgctttagg caattagatc
     1201 catgagtttg aaggcatatc acacacctaa agaccgcaaa gtggtttaat atgctataag
     1261 gaaaaccttt acaactctgg aaactaacaa agtcaatcat tttatcctaa tgagacaatt
     1321 gtaggataaa cagcaaaact aaccatgtaa tctgcaagaa aggtgcattt cttgcacatg
     1381 tttaattggc caatgcagaa cgcagcatta tgttgcagcc aatgttgagc aaagttcaaa
     1441 tttta
//
LOCUS       KCVB01000247            1396 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11529pri ultra-conserved element locus
            uce-1278, sequence.
ACCESSION   KCVB01000247
VERSION     KCVB01000247.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11529pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1396
                     /note="ultra-conserved element locus uce-1278"
BASE COUNT          435 a          247 c          260 g          454 t
ORIGIN      
        1 taatatgaca agcttgttag caaacagtca tttccagccg aggttgtgtt tgtgtcatgg
       61 tggatgtttg actgatattc agagttcttt tagctttgtt tttattctcc accaaaatct
      121 gaaatatcag aagtatctaa cccttcagat ttttactttg gactggttgt gtacgtacag
      181 aaaatattaa acacagtgat gtgatccatc gttaatatca aaatattgat catatacatt
      241 tttaaggttt gaaggtatga agataatact ctgctcttcc tgtttgtcat ctgcactcct
      301 gaacaaattt ttttttagtt acaatagtta aaaggttgta atgctataga agccttgtac
      361 atttttatac tatagtatgc taatagcatt ttgatgagtg agatctaagt gagaagtgta
      421 ccgaattgaa ggaaaaggct tgaaatgcat tgttaacggg tcaaagagac accacttatg
      481 gccatgatga tgatatgcaa ggtgtggaaa tgcatttcca atctccctgg cttgagccca
      541 tcagtttcca ttcagaagtg tgtgcacttg ttcattaagc ttatgcgtat catcaactgg
      601 tgcactcatt ctatcaggca gatatggcag ggataaaacg atgtgaagga tggaaattca
      661 aacaaccatt ctcatttgca aaggtcatta aagcctggca tacattagaa aatctgctaa
      721 ttatgaaatg gcttaactcc acattcagtg ggattatgtc tgctcattta gaatatgttg
      781 atcccctccc ttactcgtca caagacagtt gatgtatggg acttgtggca cagaactaat
      841 gttcccagtg cttgtcggtc atcttaactg actgtaagaa gtccataaaa gtggccgtct
      901 tgttttgaat gaatatctgc accaacacta caagacacac acaagtttta cagctgctgg
      961 agaaatctgt tgtactgatg atatatggct gagccacttc atcttcttta ctttagagtc
     1021 actgagcaaa tgaactgtgc atcaacaaat aataatcaca gttaaactga agattaagac
     1081 ctgaggatca agttttctac ctgcaacata agtcgagaat aacagtcatt ttctctgaca
     1141 tgtttttcac atgcagttat aatctgctcg caaacattgg tgctgcaagg catataatac
     1201 acagtcaaat aatataacat atatatgact ttaaaaaatt aacgctgtaa cttatgggac
     1261 tagtccttag ttactggctt gacagacttg ttgttcggga gtaaaccaag ctttataata
     1321 taaaaacagt tttcaaatct gacaactttt ggcttcattt attttatgtt aagacaatac
     1381 tacagagaaa ttgtcc
//
LOCUS       KCVB01000248            1504 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11564pri ultra-conserved element locus
            uce-1184, sequence.
ACCESSION   KCVB01000248
VERSION     KCVB01000248.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11564pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1504
                     /note="ultra-conserved element locus uce-1184"
BASE COUNT          491 a          296 c          293 g          424 t
ORIGIN      
        1 gttctttata aattacttat ttcaatttaa aaaatttcat gcccattttt atgcacttcc
       61 taaccataga aggggttagc aattgaaatt ataattaaaa caataattgc ttcatatgtc
      121 taagtcaaat aagcaaacca gtaagtttgt ggccggcaaa aactatactg aattcagaga
      181 taaatgtttc cttgagctga gatgtgatgc cctgtagaaa gcaacttact acagctgtgt
      241 tatagctgca tgcacagagg cagcggcatc tatgaaggca tttcagaaag gaggttacat
      301 ttccaatgaa ttttttagtg ttttacgggg ccaatcttct agccagtctg agagtattgc
      361 ttataaggtg caactcctat caacactaca caagctatgt gtttatcaca gactctgagt
      421 catatgccca attctgatat gagctccagc attgcttcag aggctataaa ccctataagg
      481 gagtgaggag ggaagaagca gtatgataac tcagagcgtc ctgctgtaat tatggtattc
      541 atgaaaagat gaaatagagt gcagactaaa tctgtagctc caggcaaaat caacagcttg
      601 gcattacgcc gtgacccctg cctatctcga ggatacggca aacataccaa aactttgctg
      661 cttttttcat cagtcaagag tgagggaatg aagtgcatcg attgcttgtg attattaact
      721 agactgagat aagccctctc cttattatgc agattactga ggctccccgc tccagcatat
      781 tgtacagatg caagggagca gctgctctta cagatggtgg gtacacggat ctatcattat
      841 gcctccattg tctccacttg aattgtttgg gggacaacag aactgcatca aagaggaatc
      901 cgactgcaat gaattaagaa agagaaacga gaaccatcaa ctaaatttat acaccggagg
      961 ccgtggaatg gagaggagcg taaagctgaa gctatttcat atatacaacc atggagtcgc
     1021 ccagtcatgt atactgcacc caaatctgtt ggatgcagca ctgtgcacca aacatatgtt
     1081 gttttttatt agcaaactgg tattaatcag gatgcagata ttaatttaaa taataagaaa
     1141 caaacaattt acaaccagat aaaaaatgaa taaataagca agtaatctgc tcagaaaaat
     1201 atggcagtct atttaagtgg agcttttgtt ttttaaagaa attacttaaa tttctatttc
     1261 atttagaccc cctcctcgat agtgcaaatt aaagaatcag tcatcatcat gacacttatt
     1321 tgctttcaaa taccacactg ctgatggctc cagtattaaa aacattacat cttctcacaa
     1381 agccaactac agcaagaaaa gaatatgcag aagagtgtca attttgccct taagggaaac
     1441 ctctggttgt ccatttgact cgccacacct cctgctgtgc agtgctgaga tcaggacact
     1501 agta
//
LOCUS       KCVB01000249            1256 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11602pri ultra-conserved element locus
            uce-1320, sequence.
ACCESSION   KCVB01000249
VERSION     KCVB01000249.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11602pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1256
                     /note="ultra-conserved element locus uce-1320"
BASE COUNT          327 a          286 c          269 g          374 t
ORIGIN      
        1 ttcataacca gaaggatagc aggaaataat gacaagtgct ttaacacaag ggaggcgggg
       61 cccttgtgta attagcaggg gcccaggcaa cttgtgtatc agagctgagc ggtctggctc
      121 tgatgttctg attgtgatta tcactcagtg atgaactctg accatttcct gcaaagacag
      181 ttaccagccc cgggccatca gttatgttga accgtggtac taccatacat gtatgcaaat
      241 ccagacactt ttactgatac atgacaacac tgcatgactt acaaaacatc taatctaaag
      301 ccattttaaa tatcacttaa ccaaatacgc ctgctcaact gcattgttcc tgtaggtatt
      361 taaatccaat tttcagctga aaagtgaata tctgatcatt aaatacgata agtatttgtt
      421 tctgatgatg gaagttttga aatcaagtca atataaagaa gggcaatcaa actgataaca
      481 aagagtttga gtgtgctgat cgtctgtgct gctgtttatt taggatcgcg tgatcatcaa
      541 ccgcctggac agcatctgcc aggcagtgct aaagggcaaa tggcctggaa cacgtcgggt
      601 ctatgagcct ggaggtgcag tggcctcctt ctacaccacc aagctgctgg acaacgccaa
      661 cagcctgtcc gaggacccct ctgcatcacc acaggggtca aaggtgacca agcatgttgc
      721 agaaaacaag gagttctcag taaaactcaa cgatgtatgt tcatttctcc cttcttcccc
      781 tcctccctcc gcttcgccct aatcgtagga ccctaccact tctgctgcca tgactggtgc
      841 tagctgccgt gttcagcatg tttctctggt tttcttcctt ctttcctttg ggtatgatta
      901 gtggttttga ctgcctggca ggaggagaga ttaatttaat aacagccttc ctctcttttc
      961 ccacacactc aatttgaatt gggctctgag gctgtgatta catgagcaca aatcactcag
     1021 tcatgagggc ataataaggt tcagtttgaa gctgctctat actgaagggg tcttgcttat
     1081 aatctgattg aaattgagac cttgaatgtg ggtgttctct aatatgcttc taggcaacgt
     1141 ggctattaac ttggaaggct tcatacttgc atccgtttgt tcctggcttt tgtgttgctt
     1201 tttgataggg atctcttttt cccaaatttt aagttcttta ccatcacaaa cacctg
//
LOCUS       KCVB01000250            1794 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11642pri ultra-conserved element locus
            uce-357, sequence.
ACCESSION   KCVB01000250
VERSION     KCVB01000250.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11642pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1794
                     /note="ultra-conserved element locus uce-357"
BASE COUNT          595 a          319 c          391 g          489 t
ORIGIN      
        1 gagctgaact ctcttcttct tttccagaag cttcacataa gattaaggag agaagggagg
       61 taaggggtca tacaaggtgc tcttctctgg tttcatgaag gtatgcagta tttcatttga
      121 ttctgactgt tcttgtgcag tcttacaaaa tccaaagaat gtgaatttaa aacatgcaaa
      181 acaccaccag agagtgtaca tactgttttg atgcacttat gaaggataga ttcgtggatg
      241 aggtcgagtt tgccaagttc cccgatgaat ttaatgttgc caagcatctt gatcttggca
      301 atggcacgct gctcctcctc ctcagaagta agagggttgt catgtttgtc atagactgat
      361 gggaaaagag gaataacagt ggtcaggcta tggttaaatg tgccagaaaa atagtgcacc
      421 tcttacacaa taaacaaaga catgaactta ctttcaacat ttctggtgcg gttttcaaat
      481 tcatcttgaa gtttggaaat cagcagtctt ctgaaggtct acaataagaa aaaaacaaat
      541 actgaataaa gcatgacctc agaagcacag aacacagaaa atgagagtag gactgagctt
      601 ccattgacaa tattgcatgg aggtgttaac aatgacaaca atacaacatt aagaaaaaaa
      661 caacagcatt tacaagggga gattggtact gatttattat ggcctgctcg gcagaacata
      721 gacagccgta gttatatata ggacaagaaa caaatatact caaggcttga aaacacattt
      781 tactgtctag cacagtataa acagtcacac ttactgtgct ctgcttttgg gatgtttgga
      841 tctcagatga agggccatca aagtttggag cgtcctctgc caagcgcaga catagctgag
      901 catagagcga gctatacttc ggctcttcta gggctttgtc tacaatctaa accagagaaa
      961 gggagaagga gaaaagggag aaacccctgt gagaccactc aggtcattaa aacgtgtatg
     1021 agtcactcgg aacagccggc caaaaatgga tgaatacatt tccatttaac ggagtactag
     1081 agaataaaaa aagggtttgc ttaccagcaa gatgattcct ttaaggacga gttttgaatc
     1141 tacgcccacg ttcaggagct caaggcatag cttgtcaaac ttctcaggcg tgagtttgtt
     1201 gagtatgcta gaaaattaaa aatgagggaa agggagaggc agcaagataa ggaagtcaag
     1261 gtgattatgc aaggtcaaga tgttggcatg acataatttt atcatgaagg ttttaacata
     1321 tatttagttg catgtgattg tgtgcctgga gctataccaa cactaaacgt ctctatcatg
     1381 actgaaaaga ccccatcagg aagcaaacat ctttgtctgc atttctgttc ctaccagtcc
     1441 agaatacaag gcaactgctg agttttcaaa ctaaaacggg ttcgacagct tttccaaaag
     1501 tctctatttt agggattcta aaatgccaga gtagtgtaga caccaggcat aagtgtaaca
     1561 aaagttatgt gctttgaaac aaaaacatat cagtgtagat gattttttta cgttttggtt
     1621 ggacccagga taatgagcag aaagatccag acgctgcagc ttggcacaaa ttatagttga
     1681 gttttataaa gatctgtagg acttcacctt tgttttattt catatatagc aaaaagggaa
     1741 atggcacaaa tttaatcaat ctggcttaat tcttgtacat aaagtacata tgca
//
LOCUS       KCVB01000251            1223 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11682pri ultra-conserved element locus
            uce-786, sequence.
ACCESSION   KCVB01000251
VERSION     KCVB01000251.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11682pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1223
                     /note="ultra-conserved element locus uce-786"
BASE COUNT          336 a          271 c          257 g          359 t
ORIGIN      
        1 gacatcttca ctgataacaa ggatcttata aatttaacta cactgacact aaaccttttc
       61 tggggcaagt aggctagtaa acttttgact tttactctgt cccttgtcat ttcaacagca
      121 ctgacaatac tgattattca gcattgtttt ctctgtgtgt catttacagg ccctgtgggc
      181 aggagaatga tgcacttttg tatagtattc ctataaaaag gtacctcagc cttccatctg
      241 tacacaaagt cggactcttt cctggcacat tcactacgcc tcccggtggc agaaaagtct
      301 gacttggacg ttaaagcaga agggtgtgac agtaacagca tgcccagtaa aaagtaagat
      361 attttaagct ccatgtaaac aaatgcatgc ataaatgtgt ttgcttatac gttaatgtat
      421 ggtgactgtg tgtgtccctt tgaatgcatg tgtccttgtc cgtgtgtgtg tgagtgtgtg
      481 tgtgttgtgt tctgtgaagg tagcgtctca gctgagcccc tgcggtgctc tggcccttct
      541 gtcctcccct cataacctca caaagacact aataagcccc ccgatgatgg acgcttatgg
      601 atttctgcgc cgctaatttc cgtaactatc actgtttgaa agatagctaa gggatttagc
      661 agagggggcc aagagaaggc ccccttgtat tctgtgggaa cacaagagct gtattatgtg
      721 atataaaaaa atggaacgtc cccccttttc tctccctcag gttacgtcca tgatatttat
      781 ctgcacacag tcacatgaga ccattatgtt tatggaatat caaatgcagc ccaacactgt
      841 tgtattgagt cgagcctccg taattaccag ggggatgcac aaggtttctg tcacaatcct
      901 ctctttcaga ggtgacacct gaactttggt taatagtttg agatacattt gtgtggcctg
      961 ctgggtacaa cgtgcaaaag catactaatg ttacagtgaa atgtatcagc agtatacatc
     1021 tggatggcaa caacaatctc ccctaaaaaa cacacaccga aaagcatgct aacatgaatc
     1081 caagttcgtt tcacaacaag ctgtgaggtg gctacaaaat ttattaactt tctacaattg
     1141 attagcgaat ggattttcta tcatgcgtcc agctctccaa gcaagaggag tttgtcattc
     1201 tgagaccgta ggccccaaaa atc
//
LOCUS       KCVB01000252            1750 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11716pri ultra-conserved element locus
            uce-312, sequence.
ACCESSION   KCVB01000252
VERSION     KCVB01000252.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11716pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1750
                     /note="ultra-conserved element locus uce-312"
BASE COUNT          489 a          395 c          415 g          451 t
ORIGIN      
        1 ttcatgtcac tgcatgattt ttctatgatt aataatctgg ggttagttgt aatttctgca
       61 ctgtttagaa cccccacaga ccttagccac atcttttgtc agaccagtgt aaatatttac
      121 tgcggtgact tggtgttgct ctctgctgtc attgtaaatc tgccctccct ctgcctcttg
      181 acagatgaga gtgacccctg acctggaggt gcaggaggga ggggtggagg atggcatttg
      241 tatgttgtgt gtggatagtg tgtgcgcgct tgtgagtggc ggcctaagaa tttttttcta
      301 gagccaccag accagttata cttctcactc agtaattatt ccagggtctg tcctgtcata
      361 tccctttaca ccatccatgc cctaaaaaaa cctcaaacaa acccctcaca tgcacacaca
      421 cacgcacaca cacacgacca tcaccacctt gtggccagca actaaaggag gggatggaga
      481 ggacgtagag agctcaagaa aagaatatgg gggttggtta tggatgttaa agttgtcagg
      541 ggacagcagg gggctgccac aatttgtggt tatatatttt gaccgaagcc acttaatgtt
      601 cgactggctc ccggcctaag tgctacattt tggtgcggtt agccctttag agagctgctg
      661 cattacggta gtgaggagca gcattaacat ccactgttcc aatatatctt ctgcgctctg
      721 gatcagggcc tgataatagc atctgttaac atgcactaag taccattatg tagcacatca
      781 cttcataaat aatacgatta ggcgaccttg gggctttgcg acgagccgtg tgtttaaatg
      841 ttctcatcat ttatttaggg tgcgaattaa aggaagcgcc acttcagaga gggaggagga
      901 ggaggaggag gacatggctg gcatacatat ctacagagtg tgtaagagag agatagtgca
      961 aactgaccca gacagcacac actctcacac acacacactg tgtggttctg ctgacatcag
     1021 cttgaggaca ccataggtca agggaaccca ggcctttgat ttggtctgat taagtattca
     1081 tgcagtatag ctatgagaga aaacacttgc ctcttttctg ctgccctgac caatactgaa
     1141 aggctacata ccctaaaaac ctgaccagca cccttcaaca gagctgctgc atttatctga
     1201 caagaatctt gcaacattag cttgcgaata gaaggctcct ggtttaccag agatacagta
     1261 taaaagatat caagactgga agactatcaa agataatata gtgcaggtaa ttcagcacac
     1321 acctttacac aatctacctc tgttcacaga gtaccatctc ttgactgcta aaatggaacc
     1381 aaacaaaaag tcaatatcaa gcgaggtgcc ttaagctctc cagggctctc tgcagaaatt
     1441 cagtgtaagc cacagtggcg tacaaacaag gctcgccaaa agactttccc atggcagaac
     1501 agcccacctt aaagttaagg tcaaagacaa agaggtgtgt gtgtggggtg gtgggcttgg
     1561 ggtgttgagg agggagagaa tgtgtttgtg agtctaggct gttccaatgg attaacccca
     1621 gacagatatg ggcctaacta tcccatctgg cgctcggtca agacagattg gcaacacaat
     1681 cactcatttc aatttgccct gaaaccttat agcctggcag gcccccgctc aaatggtatg
     1741 ctaatgtgaa
//
LOCUS       KCVB01000253            1287 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11755pri ultra-conserved element locus
            uce-1099, sequence.
ACCESSION   KCVB01000253
VERSION     KCVB01000253.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11755pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1287
                     /note="ultra-conserved element locus uce-1099"
BASE COUNT          353 a          265 c          276 g          393 t
ORIGIN      
        1 ttctaatgct ctcttctgtc tcctgcttgg gttgcgtttt gtttgcttac tttgcattcc
       61 tctatgtgtg ctcataagaa agccttttta atcagctttg cttgctgcct tcctatctgg
      121 actcgcccgt cgtacgcctg tcttctgcac cttgttctct ctggttggcg tttgtaacag
      181 agcactacag ccccatttga ggtatgataa tcagctgatt agccagcgaa gtccttttcc
      241 cagaggctcc tttgtgtgca aaatcctcac actcatttga catgtggaca tcatcaagct
      301 gatgaaatgc aattgtaatg aacaagtgag gcatagcatc tgcagtggga ccctgaacga
      361 aaaggaccct tccctcctga catgctaatg ctgaaagctg ctctctgcct gtactcccgg
      421 tgggtccctc atggaaatgt gaagagatgc atattcaaac cagcgccttc tgagagggct
      481 agatgtacgg aggagagaaa tgggagaaaa aaccctgtgt ggacttggcc aaatgttaat
      541 tctgagacag attatattgc atcacgcgca gtaatgatct gacagttcat ttgtgaggag
      601 agttcaggat ctgaggttta atgcttgggt ggacgcaggt gctgagcccc agctcctcag
      661 agctgtcaga ggaaggcagg gggctgctgt caaattatcc cagttaaaaa ggctgaggcg
      721 cctgcaaata tgaaagacat ttaagaaatt aatgcattca tattcaacac atattgcaac
      781 agtgtttcat tagaggtgct ctagtcatat taaaactgac atctgacctt tcacatttct
      841 ccgaggcact aaggaacatt cttattacat acatgctaaa gtcaaagaga aggtaattgt
      901 gataacttgc aataattaaa caaactgaca ttttgcacca tcattttccc tgttatttcc
      961 gttctttggc actgcagagc agaatgatta gaaaagctgt atttgtgtga gtgagtttat
     1021 tttagaggaa aagatatgat ttgtgaaaag cctggaggct atgtgactat cacatactgc
     1081 ctaagtatac catcatttca tctcttattt gttttgcgtt atcaaggaga cttattgtat
     1141 taccattgct gctttgttac tgcataatct tgccgtgatt atgataagta tgcataatgg
     1201 agacaaattc catacaattt ccctgatcaa cattttctgc attcatgcat aaaacattca
     1261 tcataaacag atagaaggag ggaagtc
//
LOCUS       KCVB01000254            1572 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11797pri ultra-conserved element locus
            uce-815, sequence.
ACCESSION   KCVB01000254
VERSION     KCVB01000254.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11797pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1572
                     /note="ultra-conserved element locus uce-815"
BASE COUNT          472 a          288 c          332 g          480 t
ORIGIN      
        1 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgcgtgt ttgtgtgtca gagaaatata
       61 gaaagagaga gagagaggga gaggggagat atacccaagg tggcaaacgg cagctctgct
      121 ctggtgccaa aaaaattcat cacaagtgat tgttattcca gagtcaagca tattccctaa
      181 ccataaacat gcacacatac ccgctgcact ttttatttgg ttctttcata tattaaaaaa
      241 aggaggatga ctgcttttgg gttttagatt gactgtcggg tgggttaatt gctcgtcagg
      301 gtgtgttagg gggtccactt tacaaggggt tgcgcatgta gaacactcac aaggccaaat
      361 ttttgatttt gttcacagca aggggtcctg cgatttattt cgcttcctca gataaatgat
      421 ctgctgcggg accctttcaa ttagttttaa agcgcatcag ggacggcaga gcgcagacgc
      481 catgcgtctc tgaggtggag gcaaaatgag gcttcgagaa gcagctgaaa tttagaagag
      541 gagtgcggga ttggactaaa ccacataaag tgcagaaaat cccttctaat ggcgcgtaat
      601 tggttctgta atatcattac aggcggataa tggccagtta cagggccccg cgctattaag
      661 ggtttccctg gcgtgcagcg tttatgttat taaaggggga atataatgat attttagtta
      721 ttaaatgcgc gtaattaatt tatgcaccac tgaaaataaa gttgttatta tgacctcggc
      781 gaggtgcgtg gagaaaattg aaatcaaata acggttgata actttatccc aatatttggt
      841 ctagacaact ctgacaagag tgcaggcctg tagtgtgtat ctgcttcctg tcagcgcctt
      901 tcctctcccc cctcggatgg ttgcttaaat gactaattga aattacactt tcatccgcgt
      961 tggtgagaaa taattttaca agtaaacttt aacaaggttt tcatatttgg ggtcccatgt
     1021 ttatttctcc agttattatc agaccacata atcaggaccg tggtcagcac cataatttta
     1081 acatatcaaa tactatcaaa atgtccatgt ttaccatgtt cactttaaaa tgcaattatt
     1141 ttttaatttt ttccagccta attctataag tcttcacata atctaatcat aagtaggcta
     1201 catttcaaat aatcaaatgg ggaaaaacag atttattgca tacagtttat gcaaaacaat
     1261 gcattccaag ctttttattt taaaaatgtg gcagaaacaa gacttttttt ttgctacttg
     1321 ttcacaaata agaggtaggc catttgaata taattttaaa gtccccctgc cctccatcca
     1381 tactataatt gcacatactt caagaaagtc agcattacaa aatctaccaa aaccaacggt
     1441 gatttggttg tatttacaaa atgacaaact actccgctgt tacattttat aaaaaacatg
     1501 acacatgtga gaaggttaga gtgcacattt ttgaaagcag acaacattac tctgagcgaa
     1561 ccattactcg ag
//
LOCUS       KCVB01000255            1693 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11838pri ultra-conserved element locus
            uce-850, sequence.
ACCESSION   KCVB01000255
VERSION     KCVB01000255.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11838pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1693
                     /note="ultra-conserved element locus uce-850"
BASE COUNT          615 a          287 c          304 g          487 t
ORIGIN      
        1 tcttgtgata ctcaatatat acggtaaaaa cataacacaa caagaaacag agctacaggt
       61 aaagaaagta tgttgcagtg gccacaaggg ttctccaggt ataaatgctt tcttttaaaa
      121 atataatatt ttaacctgct ggacatgtaa aagcaaaagt actagataga gagcggaatt
      181 taaaattgac tgagcaactc caagggcaac cctcatatct aatgtggagt tttagcagtc
      241 gctctagaaa cccacagcga ggaattttta aaattgagaa aattgttaat ggccgcacgt
      301 ttaaaggggt aaatctgttt attacaagtg gtagttttgc ttttgtaagt ggtgacgcaa
      361 aagcaaagca gcttatttta aagacagagg cagaaaggca gaaaatgtga aatatacttt
      421 actgaacata aaacttgctg ccataaaata aaagaatatc tgaaatagga aatatatcaa
      481 tgaaaatgaa agaaaacaga tatatgcata accctcatac ttaacgcaaa aagtaacctc
      541 tctcagtgct gtatcactat cgctctctca cacacacacg cacacatgca tacacacaac
      601 gaccactaaa aacactgggt ttttgttttc ttgaagtgct tttctttctc ttttatctaa
      661 ggtttacaga ggtcgagttt ggcttttgtg taaaactgaa cctaaatcgt cacagattct
      721 tttctttgtt tttttacatt tctttctttc tctctctctt tgtgtgtctt tggttctctc
      781 gtcatcttgt cttacctggg gttgtagagg gcatgtgcgt ctctcatgtg gttggcttct
      841 agtggatcgt agctctggtg catcctccag gcgccagcgc cgcatttact ataggttgac
      901 ttggagttgt cttcactgct gctgttcacc aatggaccct tcgataagtt cattctgcag
      961 acgagggcaa agtgagacga ggagtgaaga tacaaccagt gtgatagtaa aaaaacagag
     1021 aaacagcagg aacatggcct ctctaattaa atatcaaaat atttacaaat gatacaaaat
     1081 tggaaataaa cttttgaaaa tgtaaacatt agcaaaattg tagaaatcat ttactcaggg
     1141 acattaaaat agtgcaaata atgtaacaca acttcaaaaa agaaagagga aagatattta
     1201 tagaaaaagt gaaaaaaggc aaataaagct tcatatttaa agttttgttt atttgaacta
     1261 tttctaagtg tctagttttc tgttttagca ttaaaacaaa actgaaacat taaaatatct
     1321 aaaaactgtt ctgaagatgt ttctatattc aataagactt tttcaatttt tttacaacag
     1381 gttatgacac caacatggat tcaaaaaaag aaagaggaag aaaaaacctc cagaaaggta
     1441 aagaaaaaca aagtaagaca aaaaagtttg ttctacatca aatgcagtag gaggcaaata
     1501 agttggagat aatgtcatgt ataaatgtag ttgatgcaca cacaacacac acatattcaa
     1561 acacacactt tttactggtc tttatgtctg cagttgaaac aaacaaacaa ctacattgta
     1621 tgcaaaactg ttactttcta gcacagagag gaaagagaca gcaaggtttg cagacatcca
     1681 caaaaaaaca cag
//
LOCUS       KCVB01000256            1447 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11880pri ultra-conserved element locus
            uce-1186, sequence.
ACCESSION   KCVB01000256
VERSION     KCVB01000256.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11880pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1447
                     /note="ultra-conserved element locus uce-1186"
BASE COUNT          429 a          270 c          338 g          410 t
ORIGIN      
        1 acccctgggc taggtttaaa ccttctcttg tattctatgt aacaaaatga atagtttata
       61 ttttaaaaat ccgtctgaaa ttaatcaaat ttaattcctc tttgcgccgt gcctaagtac
      121 atgcacacgg acacacagag acaaacacac accacggtgt gtctgtgtgt gtgcacacaa
      181 ggcaacaaag caatccgtac acactgaaac aaaccctatt aaaagaattt caggggtcga
      241 ttcaaatctg gctctgtgaa atagctcacg cctgaaccag cggctgattt catgtgccta
      301 tttgattgtt tagtgtccct ttatcggcgg tgatgtcatc aaagcgagtc cccacactac
      361 agcaggacct cactggcttt acagttaagc aatccccaca atgctggctg aatgctggag
      421 aggataacga taatgatgat ggaggttatc gtggagtaat tactgtgcat ggaggattcc
      481 gcgcagtgtg aatgagtaaa acacccgagc gagagagatt gaaagaggga atgaggaaga
      541 aacattgaag gggaggggga gtgggggagt tgaatctcct aatgtgtcgc ttgattgaag
      601 tgcgtaaggt tctgagctaa ttacatccag aaccgcagcc taggaacaga tgccacactt
      661 gtgcatcaat aaatgcttat aagtaactta tttagggctg cctgccatgc aaggaaatgg
      721 catgacttaa agcacaagcg ccccggggct attccccggc tctttgtctt gtcatcctac
      781 atcttttaaa tctattcacc gcttcaaaac aacgcaggca gacagcaggg caggcataaa
      841 aaaagagaag aggaagaaaa aaaaagatac aaatattcac actttgcaga cagtattatg
      901 tttgccgttt gggttgtctg gcatcaaaga aatgctgtct tctcttagat ttgtgagcgg
      961 taaaggagtg gtgagagcgc tgtttttaag gccaagttcc tgtttgatga aatgcagctg
     1021 ttctttaaag ttttaagata aatatgtttt gacttgagtg tagagagata cattagcttg
     1081 acaagtcggt gctatgtttt atttagctgt tggtgtaata aacagtatta ggcctaaatg
     1141 gctgaaagtt gtgtgttgta gaaaattgaa cgttccttcc tcttttatga ttatgtttgg
     1201 tgtgaacaca agagaatttt cttgttgttg tttttgtggc ggagcatatg ttgacatgct
     1261 gtcatcttta gtgaccgctg tgagtgacag attcaaacag acaggttgac tgccttgaca
     1321 attccacagt agagcacata tagaacagag tatgtaatca aagccagacc ttagctttct
     1381 gcaaagataa caggcaatta gtgttgccac agccatagat cattgttatt cagtgcatac
     1441 atttgat
//
LOCUS       KCVB01000257            1397 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11925pri ultra-conserved element locus
            uce-699, sequence.
ACCESSION   KCVB01000257
VERSION     KCVB01000257.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11925pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1397
                     /note="ultra-conserved element locus uce-699"
BASE COUNT          417 a          273 c          290 g          417 t
ORIGIN      
        1 ctggtaggag taaaggcggg agagggggtg gggtgagtgg ggtgagtcgg gaggcctaaa
       61 tattcctgaa agatgtgagt ggccaagaaa ttcataaaac actgtcgttt tccctgacac
      121 atacgctcgc tcgcttctcc ctgcggatct cccctctagt tgagggaaat gtgcccttga
      181 cagtcctaac gtcagctgtg attttgtcac tcaatgggca aaatacattg tggtgctgca
      241 gacctcttgg atgctaataa ctagaggggg cagtctaccc tcgtgataac tccacagtct
      301 agtgagagtt accagctctt taacaacttc aacgaagaat cccccacagt gttttggggt
      361 gaaaaattgc tacagtgcgc tcctgcacat ctgacttcat tatattatct cttataggcc
      421 ataaagaatg ttacacagaa tcttaactag ctcattattt ttgcaggaac agtgagtttc
      481 ctctctttag ctgcaaacag ctgcgtacag aagaaaatta aagcaacagc tcttgagtgc
      541 atacacaaat gaggttgctg acactgttcg aaggggatgt ctacttcagt gagcggcaga
      601 ggtgaatcaa cagattcggc tgctaatcga agactgaaaa aagacggacg tatacagacg
      661 caccaacctg tgagaggaca tcagtaatgg gctcacattt tcctaatctc ctttgatgga
      721 ttcaggccat gattttcatt tagtccaaaa gagataacca aactgtcagc tgtgctgctg
      781 ctttgcttta atctccgcct gcagccaata atcaccctgc aacccgcaac cctttgtcaa
      841 ttgttaatct tgtattgaag acatttagat gccaggatgg tattctgcat cctatgataa
      901 tccaggagcc cactgaggag aagcaaattg gattcattat taaattttgc attattaaaa
      961 agacaaatga attgagctct caaaagaact tgtggaatca atttaaaaat aatcagtaac
     1021 atgcggatac ataaaacatc tgcagactga tagaaagatt agcaatgctt gatttttcta
     1081 atactatgaa gatcctcact gtggtagatt agagccattt aacttaaaga catttaacac
     1141 atgattatgt gagctaattg tgacaacaca cgtatatgtg ttccttcaat gccagtgtat
     1201 ttcaatggca gaatatactg tgtgagtgtt atttctaaag agatgtggat ccctttagtt
     1261 tttgttgtct gggtttaaac caaacacatc actgacttta caacatttgt atatttcata
     1321 ttggttttaa atttcaatta ttatttggaa tgtgttgtac acttttctat tttcatgtgg
     1381 taatttcaca aaaaaca
//
LOCUS       KCVB01000258            1356 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 11981pri ultra-conserved element locus
            uce-520, sequence.
ACCESSION   KCVB01000258
VERSION     KCVB01000258.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="11981pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1356
                     /note="ultra-conserved element locus uce-520"
BASE COUNT          407 a          341 c          283 g          325 t
ORIGIN      
        1 aaaactgttt tgctctctct cccctgccag agaaggatga agaggagcac aaaaggactg
       61 aaaggtttaa aagcaaaaca gtgctaaaat ggttgctttg ttaaaaacag cacacatact
      121 gtagaaagtc ttcactctct ccacctgtcc aggaagctct gtagggtaaa gcaaggagta
      181 ccgaggccag taaaaggtct gctgctcgct tgctgcatga gccattaagt tacaacactg
      241 caggcttcct gttttcatgc ttgaacacct tctacatgct ttcaacgagg acaagactaa
      301 acaatctcga gtgaaacacc agggaagaga aaccagagaa gacaaagagt atttcaacca
      361 cagaatgctt taaaccacaa caaatcacac gccactttga atcaagtttg tgaaagttaa
      421 aacaggaaat ctaaagtcag tttcatttca ctagagaaag gagagtttcc tccgaggagc
      481 ccaaccagac tcctcctgtt tgtgatcaca gtggccagcg agctctgcct cagctctgag
      541 ggactcctgc tctatggtaa tcatatccta tttgaataat gaggcaatac caccactcct
      601 gctgcccatt ccctctgcat ccttgactgg actctttgat agacactaat tctagctcca
      661 tctataaagc tcctctcatg catttttaat agaatctatt actttaggat taaattataa
      721 aagactgtcc tccttaaatt tttaatagtt tgcataattg gtatcagggc ctgaatgcga
      781 accataaaaa cccagtttgt acaggactgc gctgcggaac gagcgagaca aaaccacaca
      841 accccgctgc acaaaaatga cacacatggc atgccactat cacaggcgag cacacatctg
      901 cacacgctcg tgcacgccca tgccagcaag gccattatag ctttgggttc attgccgtgg
      961 agaataaggc ggcgtgagct cagcacggtg aatgaagggg acaataaggt cagggaccat
     1021 gagagctgct tctggctgct gagctcttgc agaagcctga ggcaggagga ggaggagtca
     1081 cctgatggaa acaggagaca gaaaccaggt gtccacagac acctgctgcc actgatgtca
     1141 aatgcaaccg caacactcac tggggcaaac agcaaaaact acttagcagg tttacagcct
     1201 ccctttgtcc ccacagtcac tgataactgt gttcagtcag cttaaatgga aatgtatttc
     1261 ccttctctgt tttccaggca gatcagccat gagttaaatg tcaactgcac tctttcactc
     1321 tcactctctg catctccagc tcagctcttt ccaata
//
LOCUS       KCVB01000259            1781 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12025pri ultra-conserved element locus
            uce-565, sequence.
ACCESSION   KCVB01000259
VERSION     KCVB01000259.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12025pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1781
                     /note="ultra-conserved element locus uce-565"
BASE COUNT          432 a          507 c          351 g          491 t
ORIGIN      
        1 caattatagc cctcacgaca gactatttac ctgctgtatt tatggcctaa ttaatgggct
       61 cttacgtaaa ccaccttaca tatccaactg caggtaagga agtatttttg ctaagatgga
      121 tacatccacc tccacaaaca ctgaagtaaa ggttgggtgt tgctaactcg cctgtcttgt
      181 ctttctgcca ctcattttct tccttcctct acccatttca ccctcctttt tcttgctcct
      241 ctgtccaaat gcccgtttct ttcattctct gtacctgccc acaggagaca gacatcctgg
      301 aggaggagaa ggcagagctg gaagctgaga tctctgagct gcagaaggag aaggagcagc
      361 tggagtttgt cctggtggcc catcagccga actgtaagat cccgtaccag gaccagcctc
      421 agcagagctc agcgcagctc cctccagtcc agccccagct cccaccgcag accttacaac
      481 ctcctgtctc cattgtgggc ttggctgtga aggaggactc tttctatctg cctcctgcct
      541 acacagccca tccggcctcc acacagtccc agcctccagt tcagcagcag caagtccagc
      601 agcagcctca gccagggata atgcaggagg tagagttttc tagttctttc tatggctcaa
      661 gtgagccagc accaggcggg ccatgcctta tggccagcga cagtggtggt ggtggtggtg
      721 gtaaccatga cgatgcggcc attggcagct acaacacctc atacacatct tcatttgtgt
      781 tcacctaccc agagggagcc tgcggggtca gtgccaacca gcggaacagc agtagcgagc
      841 agtcatctga ttccctgaac tcaccctcac tgctggcact ctgactgacc gacagacgca
      901 cgcgcacaca catcaacaca tgcacacatt atgctcgctg gaagtaaagg ctgaggcaag
      961 tacatgcaca gtacctatca gtctcaaaga ctgagaaaaa aaaaaaacaa caaccaatca
     1021 tttgtttctg attggattaa agtttacaca ttctgaaatg cattccaata aagagccata
     1081 caaatagttg cacatgactt acctttaaca ttgagcttaa acaataaatc atttctcgtt
     1141 ttgtattatc ggaatacctg caactggaca acatatgtgc ataaaaaact gagtcacaaa
     1201 atgaagtcca agttgccagt ctgaagtgct ctgaattacc agattcattt ataatgaata
     1261 ttctcaatct tcatgtacaa tacattgagt gagataccac aagctcactt tacactccag
     1321 caagcaccag tgcccctacc ttgttccctc tgtcggttgc cccttttcca gcgctcttgc
     1381 tctcatcctt ttccatcctt ttctcccttc ttcctcttgc tgaatgtatt tgtgcaaatc
     1441 tgaaataggg acaaaggacg ctttgttaag ggtcttttct tgtgggagac agctgatttc
     1501 tcgctcacct ctaacccttc atctgctcga cttccctctg cttcttcatt tgctgcttgg
     1561 acttggcaag ttgggatctg agtatcacag cctccactaa atcttgtgct tttttttatc
     1621 tcgttattaa aaagtcctct gccgtctgcc tacctgccat gcgacccatc tctgcccatc
     1681 tctgtttctc ctcttctgtc tttctgtttc tccctctctc tgaagtcttc gacacctgag
     1741 agcccgaaga ccccctccag cccttgggac cttgagtgaa t
//
LOCUS       KCVB01000260            1515 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12069pri ultra-conserved element locus
            uce-1287, sequence.
ACCESSION   KCVB01000260
VERSION     KCVB01000260.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12069pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1515
                     /note="ultra-conserved element locus uce-1287"
BASE COUNT          483 a          371 c          298 g          363 t
ORIGIN      
        1 gttgttctct ttctttcttt ctgtggctac ctatagtaca cactttactg tatcccgcca
       61 cagctctgtg tctgtacttc aactgcgaag aaatctcaac cgcagctgta acactcatgc
      121 ttcacaaccg ccaacaaagc tcccaaaaga aataccccaa agaagtacac tacttttttt
      181 tttcaacctc caaaaaacca cacatgtaca gagatccaag ctgcgctctt tctgtgttta
      241 tcaagcaccc ttctaagcag ggtcctgctg aaaagcctgg agaaccagag gcagacatgc
      301 tgctctctta gtgcccagcg cctccaggct gcactgaggc agcgcaggaa cacaggcacc
      361 attagatact gtttaccttt cagtttgggt gatattgaat acattagccg attgaacctc
      421 ataagaaaca cagccgagtg acaaggccca gtcgtctttg cgactaccat ggtaactgag
      481 attacaggct aacaaagagc agacaggggg aaaaaatatt taaaacccaa atagtgatac
      541 ataaattaat taaaggacaa aaaaacaagt ggagaattaa agttggaaca tctgtcagcg
      601 acttagcttc ccaacgtaat accagtgtta gaacatttat acccttgaag ttaatgatcc
      661 cctctttaat tgacagtcaa ggaaaactgg ctgcggcagc atcctgccga gacgccgata
      721 agaggccccc ttcccccctc ccaaaaataa aataaaaaaa aaacagttta aagaaccttg
      781 gcactaatcc ctattgtcat cccagtgcag cagaaggact tacaaacaca gataactcca
      841 ctgaagctgt ctatttctca ggagttatgg ggacagccga gccgctgcgc gcctcgaatc
      901 agtcactcag attagcccaa tccctctgtg ctgtcagtca gccgagacag tagcctgtca
      961 ccgcatttac atgagtccat ctcatatgtg acaatgcccc ctatatatga caatggctgc
     1021 gaggaagggg tccactcgct cgcagatagc tggtgtacag tatggcagac gggctgttgt
     1081 tggattaagc tcatggtgtg cgctgcgctc atgtcttcgc tgacactccg acaacaagtt
     1141 taaggagtct taaccatagc tgttttttcg atagtggaga gaatgtggga gatgtatgtc
     1201 ggatgaaaca cacacataca cctacactga caaacatgga tagacacaca cacacacata
     1261 ctgcacacac acagggataa acttcaaaaa acttaaaagt acagaatgag tgttggatga
     1321 aaaccccaag cttacctagt gtatgtcttc cgtaaagttg gcttcaagca tgactttaat
     1381 ccacctacat cacttaacat gatgaagctc ttagaataaa agagaaaaca acataaagct
     1441 aagtacatcc tatacgaaac atctggatca gtctccatct aagaattaac acaagtatga
     1501 gttagattaa aaggc
//
LOCUS       KCVB01000261            1447 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12112pri ultra-conserved element locus
            uce-149, sequence.
ACCESSION   KCVB01000261
VERSION     KCVB01000261.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12112pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1447
                     /note="ultra-conserved element locus uce-149"
BASE COUNT          428 a          293 c          323 g          403 t
ORIGIN      
        1 aatatgcaca aatgtatgcg agatgaaaca tgcctctttc tctcataaag atgaaaaaaa
       61 gcaacaaaag ccttccctag gcagagagag cttaactatt cttctcagtt caaagtcagg
      121 ggtctactct aaatatttgt gtattaaaca taacacatac tgtaggatgg aataaatgga
      181 ggactctaaa agaagtgcag agcctgcagg agaaaacaac gtggaactgc aatgcttctt
      241 aatgtcagtc cgactagcaa aacaaccatg gtgaaaaaca agccaaggct tcaaatcctg
      301 tagaaagtat aaatcagtgc aagtcagagg ctgcgtggct acattttgta aaggttctgg
      361 aaggattaag attaaggatc acctcagttg taggacaaaa aattaggcaa gaatttaatc
      421 actaatgaca gctacacctc tgttgaaaca gttccatgtc aacacttaaa gctttcgagg
      481 tacgcatcaa gggccaagga gtctaaggag gcaccatgca gcatgattaa atctaaagat
      541 gctgactctt acactgtttg tgtgtttatt gaggaaagtc agggggtgtt ctgagcctca
      601 gaactgcatt taaagctgat ctggctcctt cagacagtgc tgcatccgac ttcctgttgt
      661 cgactcgcag ttgccacaga aaaattgctg ctgcagcctt agacaaccta cagcactttg
      721 tatgccgaaa gaaaagagtt aattagaaat acaaattctc ccagattgtt attaatggga
      781 actgtagagt aagcaacaca tattttgtgc ccataagtaa ttctcaggta tctttgatct
      841 gtggcccaca gcggatgctg ctgcgtgccg ctgtctggct tctgggactc tgccagcgcc
      901 agacatctgg ctactttgtt atcattggct gatctgctcc cgggaggcca ctcttgtgac
      961 accaggcaga gagtgtcctg gcaaggaaaa ttatcagcac attttgagtg gattagagtt
     1021 ccaaagtggt cttcaaatct ctacaggggt ctggtgagcc atgctctact gggggttcct
     1081 cagggctctg ttttgacaac actgtggatt tgaaaggcag tgagagtaca gctcccactg
     1141 ttgcatcaca ggtttcaggc aacatcttag gaagtgaaaa aaagcccccc caattatgat
     1201 ctgtcagatg tagaagggta aaaaatgcac caaagacttc ccttgattag tgtggagatt
     1261 cggagtagca gcaaatgttt gtactacaga gctgtatggc gtttatactc cagtgtcata
     1321 tcttgcaaaa atgtattaag tctgcatttg tttttactta ctaatgcagt tttgttgttc
     1381 ttcaaattct gcagctatga acattttcag tcacgttctt tgccttgagc tttttttaac
     1441 tctaaag
//
LOCUS       KCVB01000262            1536 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12153pri ultra-conserved element locus
            uce-598, sequence.
ACCESSION   KCVB01000262
VERSION     KCVB01000262.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12153pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1536
                     /note="ultra-conserved element locus uce-598"
BASE COUNT          514 a          295 c          314 g          413 t
ORIGIN      
        1 tttctaaact tgcctgtgga ggaggaagtg tctatcaagg caaaaacagt gcatactacc
       61 tcaacaatgt cagcagacca ggcaaaatta aaaaaaatat atatatatgc cacagcaacg
      121 cagttaatgc atgagtcacg gtacatagat acctgtcaac gttcttatga caaacatcta
      181 tctactgctg aggtgagagc aatttcaggt aatagaagtg taaaaaaaat gtctatcaac
      241 aaggacgttt gtttgtctga accactcagg atcactgcac gcacataatg tttctaagag
      301 ccttatagac agttggagag agacaggaca gcacaacttt gagtcaacct ttttggaaaa
      361 ttaaacccac acatggcctg cattttaagc ttttttcccc cataaaacaa aaacatacag
      421 aaagtcatct caaccctttt ggtttaaacc accccaactt accaacacag gaggtttcag
      481 tttgttttat tgcaaaaaca caacaggcaa tagcatttta aaactgagat gatttacatg
      541 gtaatgtcta cagtttaata aacatgagtt ctaaccacat ttaatgtaga tacagggctg
      601 gaatgaccat atgtataatt acctcactaa aaacaaagca tttacaagaa aaagaaaaac
      661 aaaaaaatga aagaggacgt gttcgccggg taatctgcca tctatgtgaa acactttgaa
      721 accaaggaaa tttaagatct tcatcaaggc gtctgcatcc aaacatttaa caaaggattt
      781 tgtttcgaca atgtagcatt tactttatgt ccacttcaca ttactggccc tgtgcaaaaa
      841 aatacataga agatacattg catgttaaca gcagaaacag tggactcagc ttcccaaaat
      901 taaaagttta aaacctgtgt aaagaggaaa tagttttggt taagtttctg aagctgctgc
      961 tgtcactgga accctgtaaa acctgaggaa tgaaggcggt tagggcacaa aagaattgag
     1021 tattcagtga atgtggaggg cagattctta tcaggagtgt acctgagggg cttgtggtgc
     1081 tgcacccatt gtctgagggt tggctgtgcc atagtacgct gcctgctggc ggtagtactc
     1141 tgcccagaga gcgctgtagt cgggctggcc tccgggctgg gaggctgcag gtgcagctgc
     1201 ggcctgaggg gccgcttgac ctggagggta aaacaagaga acacatttgt acactggcct
     1261 gtcgaggtta ctacattcat cccatgtaaa tgctggatag atgtacaatt aaccagcaac
     1321 agtaccttca catgcatccc aacatacgag gcagtaacga cagactttca acagctattt
     1381 ccaaaacaga catatctaga taacagttta agtctatgag atgttttttg ctttattttt
     1441 cttttatatg aaagtaaaag gctgtttttc atagagataa aatttaaagg gaaatttttt
     1501 ggaaaaataa atatatcaat gtctaacatt tgagct
//
LOCUS       KCVB01000263            1725 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12230pri ultra-conserved element locus
            uce-664, sequence.
ACCESSION   KCVB01000263
VERSION     KCVB01000263.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12230pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1725
                     /note="ultra-conserved element locus uce-664"
BASE COUNT          470 a          361 c          372 g          522 t
ORIGIN      
        1 ctgtcctctt cctcctgcct tgtatttgca tttgaggaga cggccccctc tgtctcagca
       61 gggggttgaa cacatcaaag acggcaacag gtgtgtggtg tcaaacacaa tgccaggacc
      121 cccgttgcgc tagggcgtct tggataaagc ctgtcagagt cctcaaaaac tcccacaaag
      181 ttacccagga aagggtacat gcacacacac agacgctgac gtatatttga gatctctaat
      241 gaagaatgtg tgattgccag tgggaggtag aagaagacag cgagagggtg agagagaggg
      301 tggtggtggg gttctcaatg aaggagtctc cagcggttgt gttaacccct gatgcctcag
      361 cacactgtga gtcattctgc cctccgaggc ttgtctctgc tctgctattg ttgatgttat
      421 tgttattgag gaggttcagt atgctaaaaa gacagagaca gagagaaaga cagagagaga
      481 gagagagagc aaaagaggga gagaaagaaa tagtgtgtgc cacatctcat tggcataaat
      541 acttgtttac aacaagacat aaattggcac cctgtcagtc agtctctcta tttctctctc
      601 ctttaactcc cttactctgt ccctttctgt ctctatctgt ctctctctct tcctctctct
      661 cttcctctct ccctctgtgc tgctaagcgt attacaacac tccctggttc aggcctctct
      721 cattaccata ttctaattac attcgttaag gggagcattt gggagtttaa tgaataaatt
      781 tgaaagatgg gtctccgtgg cgatctttag gtttgtctcc tcctcaacca actgaagatg
      841 ggagcatatg tgtgtctggg tatgtttgtg tgtgaggtta cgcacctgtt tggtgtatgt
      901 gtgtgtgtat gtgtgtgtgc tctcagtaaa tatcactata gctccttttc agaagaaaca
      961 aacaaatcta gttaactgct ggagctcacg tggagttctc tctctaaaaa aatcaattct
     1021 gaattctgtt tgtctctgcc aaataaagat ttcatgtcat ttgtttgttg ttttggataa
     1081 aacttggagc cacccatgtt gaagtcatgc atatttaact ctcctcaagc ctcaaaaaca
     1141 aactgccaaa gacaagcatc aatttacaga tgaacaaatt gtatgcaacc tcgggtttat
     1201 cttttccacc gacatcagac ctggaggctt catactggga gacgggaggg tggagttaaa
     1261 acaatcataa gcaaaagtaa acattaaagg ttatcaacca tgctccggtg actggcttcc
     1321 actgttgtgc agtttgatct gtctctgcct aggacgaagc cctgggctgt agacccgaat
     1381 agactgttgc tatgggcctt gcactcactc tttcagggta ttgcaaaatg ttatatacct
     1441 ttcacatggt ttaataaagg agtcccaaat gcattcccaa ttctgatgat tgccagcttg
     1501 cgcttcaagg agtcgaggag tgaatgagca aaattcactt cttgctcaga attacacttt
     1561 ttaaaagcta cagaaagatg catatttact tttcatgtgt tattgataat tatacaatat
     1621 caaaaacaac agaaaaatat gcagaatcta atcttttact gcgactgcat ttgattaaaa
     1681 ttatgctatt tttcttcttt ggttaaatac attatttttt aaatt
//
LOCUS       KCVB01000264            1499 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12276pri ultra-conserved element locus
            uce-485, sequence.
ACCESSION   KCVB01000264
VERSION     KCVB01000264.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12276pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1499
                     /note="ultra-conserved element locus uce-485"
BASE COUNT          378 a          393 c          303 g          425 t
ORIGIN      
        1 ggtcttgtgt gtttattttt gtgtaatgac caatttaagg tttgatcttg atttttttta
       61 gaaagagctt tatttatttt aaatcgactg acagtgtttt gttaaaaatt ggatgtaaag
      121 tgttttattt ggatactatt tttggccatc tttaacatca aaaaatataa agagcattaa
      181 tttggttgat gggctccaaa gcatgctgaa gttaaacttg ctaaagtatc acaaattatg
      241 aatgtcagct ccttgtctaa tcaagacaga gtccatcctt tcaacagcag gagcaaagca
      301 gtgcgtgatg ttgcccttgc tcatcccacc ctgtcaaatt aattagtacc catcattaat
      361 ttggtaccaa aactggctgt gacctcctta tccagctcca cattggtatc aaatcagccg
      421 tgtatacctg tcagataaat tctcatatcc tgtcccattt cttattcagg aaatctgtca
      481 gccttacccc ttctccccac aataaaaacg atcaaccagg aaggatttgc gtttgacagt
      541 tatgacaaag cagagggggg cttgttcagt ttcaaggtgg aatgactgca ccccctaaag
      601 gtcttaggcg gaatttcaca gcggcttccc taaacctcct acccccgtcc accccacctc
      661 caccactaag ccaccagaca gtcaaacaga gaggagagag gggagagcga gggtaaggga
      721 gaagacagag gggtgttcgt gccaccaaca tcatcctctc ctctccacca gcctgctctc
      781 gtctgccagg gtgcttatga tccattttcc cccagatatg agaaagttgc tcgctcagag
      841 gaagaaagca gtcaatcaat gctactgaaa tagccccact ccattagcag gcggtaacag
      901 gagctgtgcc cttgcttagg ggactttgga tcaggcggct ccagtcccct agtctctctc
      961 tcctgtcttt gacttactga ctctctccct ctgcccagcc tgcccccgcc tctccacacc
     1021 cttccaaact aaaaccagat ccagatagtt ctagagataa tatggcatat cttcttattt
     1081 ccgcctcttt atttcctctt acctcaacac cagccgcaac agtgcacctt ttgtcatttt
     1141 aaaagcctgt ttgcgcccat ctaaatatcc ttgtcgtttt cttgccatgt cagccaggcg
     1201 gtcagtcagt cagtcagcca gtgccctgtt tgcctgcctg gccctgatgc ctgtctctaa
     1261 agacctgtaa ttgtattaaa agtactcccc tctgcaggcc cttacacccc gatctttgtg
     1321 tcgccgaaag ccgatacctc aaagctcctt ttgttttata aggggaaaat cgtttgggag
     1381 tggaccccgg gatctccacc tggcgcgcat gcctctctca actgcagatt attacaagga
     1441 acatctgctc gggggtcacc agcagtgtca gttggcatag aaagataggc agcatggat
//
LOCUS       KCVB01000265            1351 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12344pri ultra-conserved element locus
            uce-15, sequence.
ACCESSION   KCVB01000265
VERSION     KCVB01000265.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12344pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1351
                     /note="ultra-conserved element locus uce-15"
BASE COUNT          380 a          271 c          366 g          334 t
ORIGIN      
        1 ataaaggaaa tagagcatga aggaggagga aatatggaga ggaaaaaagt aagtgaaaga
       61 agaagagaga caaaaaaagg gagagatgag aagaaagaga gatggcctct gtaagtgcag
      121 caacacacag gcacagagat gtgagttccc aggcaagaca gcatgagctg cctatttgtc
      181 agcattgatc aacccattaa ggggctgtgg tgggaagcat attggttttg gaggccaaag
      241 gggcagagtg agacagagat aaagtaagac gtgggggtgt tgaactcctt tcaggctcgg
      301 cagccacacg tctgaaggat gaaaaagcct gtactctttc tctccaatgc ctatgcagtt
      361 cattttgcaa ggcccaacgg ggaggtagta gatgtcattt ctcttacagc atctctggag
      421 gctccactgt atgtgtgtta tcttagctct ctgccgctac aacgctaatc aggaactagc
      481 taggttttca gacagacaga atgtcacttg tcctcttcct tagtgtgtgt gtatgcatgt
      541 gtgtgcgcaa ggggtgggtg gtggggtggg agtgggtcct ggccctgggg aaaagcaggc
      601 tgcctcttcg cccagcctct gatgaattca tataattaac tctgccttga tctgttggct
      661 aatcctgcac tcagtaataa gggattaaaa tgtcacctaa gccacctgct gttattgagc
      721 ccctagactt tttgtgccct tgtgtgtgta tgtgtgtgac agtaaaagag agggagagat
      781 agagtggaag ttggggacag gggggttgtt gtaaaagtaa agtgaaataa agtgaatgat
      841 ttctgcgcta gaatcatttc gtctgtttgc acaagaaaat gtatggcgac actttaaagt
      901 agctcaaaga agcaaagatc ttaactactg ccatatgcat gtgagtgctc acaactccat
      961 agtttggcat taagctgaac tccacaattt ggcaggaagc tggagcagaa gttgtagcag
     1021 actcgctctg aaaactcatt gtcacactcc ctaaaaaggg acatgaaagt gggacatgct
     1081 ggaaggtggg agttgagaaa tctttttttc ctcccctctc acaatgacct ccttggacca
     1141 agtaaagaaa aaagaagaaa gaaaggagtg ccaattggtt ggctaacagc ccacagacca
     1201 tcggccagct tgccttgcct gcctgccggc cacctccgct cggttgccta gcaaccccaa
     1261 gtgtactttt gggttgtcta atgactcttg ttgctgcagt ggctcagggg ctactaatga
     1321 agcctcctga ccaatcgctg ctgtttaaga c
//
LOCUS       KCVB01000266            1667 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12380pri ultra-conserved element locus
            uce-1023, sequence.
ACCESSION   KCVB01000266
VERSION     KCVB01000266.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12380pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1667
                     /note="ultra-conserved element locus uce-1023"
BASE COUNT          518 a          343 c          325 g          481 t
ORIGIN      
        1 aaaacatggc aggcttgctt ctgacagcac cttgaccctt ctcactgtaa tgccctctgt
       61 ccatgttgca aagtagccat agcaggagtc cagcgcaaat tagtgaccac agagttatgc
      121 aaagatgctg aagcaccaca tccaaaatca cctctcattg gttttttagg cagaaatgtt
      181 caaggatatg aattgaatta taaaaacttg tttatttaca aggcatacgg gggttcttgt
      241 gtgtgtgtaa gtcatgttta gaacatgcaa aagcagggca aatgtattaa aaagaggtca
      301 gcagcatggt ccttgcagta aaggtgtgaa ctgagctgca ggaaacaaag cagctctgga
      361 agacgtccag ctgcaaaagt caagtccagc taaattgagt cttttctcgc tgatcagcac
      421 taagtagccg aacgtatgga ggctgtggcc tggtgaatca cagcttgcct gacagatctg
      481 aaagcagcaa acaaagtcag ctgaatcttc ttgatactgt gtcaaacctc tcattgcaga
      541 aaaacagcat gtcacagtgt taattgatat tcatcccgag attctcctcc ccgtgctgtg
      601 aagccccgct ctgttctctg gctgtggaga tgccgtttag tcttattaaa aagttgtttg
      661 attagccggc cctggaatgg aggctgtgga aaggtctggc agcagcagat aaaaggggaa
      721 tgataaatag gcgaaaagcc agacgattgc cagtcagcca ctagagcatc aaaacacatc
      781 catttctctg caaagttgat ccaattattg tcagccactc acagatagct actatgcttt
      841 actcctttca ttccaggcta aattgctctt cccccttttc gccgatacgg caatcggtca
      901 taaagggcca gccatcatta gtgctgtttg aattggagcc ctgtagtgtt tggttaagag
      961 ggtaaatgca aatttgatga aagaataagg ctgcatttac atatcagcct cagctcacat
     1021 ggggagggta aacattctgt ttatctcaag tgtcatttta tgatttcagt cgaatataaa
     1081 accgaccttg gcccactcgg agcaaaagac caaggaaatt ttctgttgtt taaacacaat
     1141 ttattcaagt agagcttgtc aataaaatcc ccttaaactt gacaaaataa aaaaactcag
     1201 gatgttttta tcaatcagtg aatctaacct tttataattg gttctctact tgatcatttc
     1261 ctgttccaaa tgatgcaaaa aagtggtcat tgtgaagtga tactcttatt taatacctgt
     1321 gaataaaatg gtgtcctcaa gggaaacgta acaatgccac gtataagaac tcaataaatc
     1381 acataattca caaaatcaaa ttgataactc aattataaaa taagaacata aaaagcctct
     1441 aaaataaaaa cctgaatgat tgttgttttt ttgcagaagc agttttcctg ctaaatagct
     1501 gaactgttgt taataaactg gactcaacag tccagctcca ctgttacatt atttttgttt
     1561 aattggtgcc catccctcat gtgatcattg cacctgtcaa tttcacatac aatattccat
     1621 ctaactggac ttaacattca atgttcatta gactctattt ccactgt
//
LOCUS       KCVB01000267            1463 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12420pri ultra-conserved element locus
            uce-779, sequence.
ACCESSION   KCVB01000267
VERSION     KCVB01000267.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12420pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1463
                     /note="ultra-conserved element locus uce-779"
BASE COUNT          480 a          226 c          289 g          468 t
ORIGIN      
        1 attactaatt acagtatgta ttgatgaagc cctgtacgca aaagtgaacc aactatgtat
       61 tgtatagctg ctgtgaatat aggctacatg attgataatt tagatattaa aaatatgttt
      121 ctaagtattg atgtgtctta atgtctttaa atgtttatat tagctgaaga tgaaagtgag
      181 gatgtattat aacaacacca ccaccgccac cagcaaatct tgctttaaaa tgtatttaat
      241 tatatttttg acctggtctt tttttttgct gcctcttttt tgtttgaatt caggcctata
      301 aaacaaccaa tttagactat tatttccact ggtaacctaa gcctgttaca tggataaatc
      361 ctctcatttt ataaatctga atatccacta gcaaaatata aacaaacccc tgatctcaca
      421 cacacacaca cacacagcag aagtatgagc tcatactgta gtttagactt tagctgtgtg
      481 cgtaaacgct ctgcatttaa attgtgtaaa tatctatacc aaaaaaaaga ccctctctta
      541 tattttttcc tgccgacaag tttttacgtt atcatactaa tcgcgtttga acagctgcaa
      601 tttgctttgg ttgaacaaag gcattctaac taggttttaa ttaagtgttc ctaatgacct
      661 taaggagatt agaggttcgc cttctaatca ataccaggca gagaggagca aagggagtgg
      721 ggaggaggag aggggagatg gctgggggct tggatagagg tagggaggaa agaggtggag
      781 agggagaaaa aaggagaaag taaaagaggt aaggagagaa gaggaagtga aaagtggtgg
      841 aaggagaatg aaattgaatc agtggacgag ggaaggagaa aatggaaagt ggaggataaa
      901 aactttgaac tttatattta aatatttgct caaataaaat aattggtctc tgataactaa
      961 aagtcataat ggctggttac ttgattgtct tcagtagtca acaagatgat aatgtgtctt
     1021 taattcttaa aggcaacaac acaaactggc agctggagta gactgtgcat ctctgccaat
     1081 gttggtattg tacagagggg taacatccaa aaaaatattt tcagtaaaag gctattatgt
     1141 agattattaa attcattttg ttgctttcca tctttttgcc cagtagaaat tttattttat
     1201 ttatttattt attttgctta gttctggaaa atgcgattgc tgtgacaaat ttgagtgcaa
     1261 taccatcatg gtagggacag ctgacaagga gctgtctcta catttaaaca ggctccattc
     1321 ataaagcaat atgctctcaa actttgagca ttgttttgat tggttttctt gtctaaatct
     1381 gtacagcctc tcatctgcca ataaaatcat tgcacattgt tctacacaat tttaaccttt
     1441 aacaagttac attactttaa tga
//
LOCUS       KCVB01000268            1469 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12463pri ultra-conserved element locus
            uce-355, sequence.
ACCESSION   KCVB01000268
VERSION     KCVB01000268.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12463pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1469
                     /note="ultra-conserved element locus uce-355"
BASE COUNT          389 a          321 c          332 g          427 t
ORIGIN      
        1 actgagactg taaaaaggtg tgaaattatt ttgctagact cttaaaatgt tactactttt
       61 tgtccccagt gcaggttcat tagggaagac agtggggctg ttgtggaaca gtgtcgccac
      121 cctctggtga cttacaggaa caacagaggg gagaaataca atgcacgggc gttttctctt
      181 cttactgctt ttcgggatta aaaaacaaaa ttaaagcgag agaaatgacg ctaaaaaagt
      241 agtgtcatgt ttcctttttg ggtgggattt taagcaaagt gctggtgtct tttaacagac
      301 tttccggatg tattttaaaa cagcatcata ttatgggcct accactgttt gaaactagct
      361 gctggcttta ttgctccctc tgctttagga cggacccagc aaaggagcag agggcggaag
      421 gttcccaggt agaaacaatg gatcgctcca aatgaccgtg agtcctgcgc gtccccgtcg
      481 ggaagaatcc aataaagctg atcatttccg ctcggccttt tgtccagagt gcagtctcac
      541 agaaagagaa gaaaaaacaa atgggtgcca ttctggaaac ggcctaattg tgtctggact
      601 gccataaagc cattaaaact ggaatgccag acagtatagc agaccaggcg ttttattgca
      661 ctgtctctgg tcgagtcttt gaagtttggg tccatctgcg taccctaacg cgtcacaaca
      721 gcactccagt tggactaaag tccgacttac cctgtagcta tacaataatc tatccgtttc
      781 tgtgcagcct taaatgagcg ctaagtgtct cggtgtatgt gacagaaccg tgtctggccc
      841 tgatccacca tccgacttag cagctctgca gcaagctcac attggatttg ctgcttctcg
      901 tattttctat tctgtcgttg tgccaaatat tttctccaac atgcagagac ttgaaccggc
      961 cttaaaaacg caagaggcca tatattttaa aactagacct cttctctaaa acgtttgtga
     1021 taaaactgtt tgtgataaga ccccgtgcgt tctgcatcca aaactcagtt atatttcaca
     1081 atatttatga acaaaatctc ggttcttgtt gtaaacaggc tggaaatggt tgtgcgcaga
     1141 ctggctctta tcatgtagct ttcggctgag cggccttgtc atatctgagc taagctaaac
     1201 agaaacacat cggctgtatt attttgggct tcagctgtcg aagccctcga tatctttttt
     1261 tgttttgtgt cctctcgcct cgcagcgctt tgcaccccat aaaaggttat agcggtaatt
     1321 gtcttttatg gctgtataca gctaacaaca tcctctgcca agacgcggct ctgtccccaa
     1381 tccagaaaca cacatacaca gatattcacc tataggtgtg tgtgttttat tttaattggt
     1441 gtgtgtgtgt gtgagagaga gagagagag
//
LOCUS       KCVB01000269            1531 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12511pri ultra-conserved element locus
            uce-784, sequence.
ACCESSION   KCVB01000269
VERSION     KCVB01000269.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12511pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1531
                     /note="ultra-conserved element locus uce-784"
BASE COUNT          432 a          336 c          281 g          482 t
ORIGIN      
        1 tattgttaga tttatgggtt atcaattttt cagtgttgca gagagaataa atgaacttta
       61 aggtgtttat tgaaattatt accaaaatag aacaaatatt ccattaaagg acaggatttt
      121 catgtctcaa aaagcgtttt ctaaatgaaa gtcaagacat gcgggtttaa aattgagcta
      181 aattttgtta aaaaaagggc acaaaggtta catgtaacca ttgtttcaac agcattgaaa
      241 acactgtatc tcatttgtaa agcagaacct aagtgcatgt ttgaaaaccg agaagacata
      301 aaaagattca ctgtccaacg ggagcccagt tttattgaga agtctggaca tcttttgccc
      361 tgcagatcca caaatcagtg cttactgggc tgtcactgct gaaccacaga gtaactctgg
      421 ctgggatcac agggagtgca atgtcctcct gcctgcagag gccctgtcca gtacaggggc
      481 atttatctcc tggcctgaac tgtctcactc aacaggatgg ccagaagtta cggtgcatta
      541 caagcactta gcgcagtgat atcaaggttc acctccgatt tccaatctca ggccagaata
      601 gtgggctggg aggccgtcat taaaaagcca ttacatgccc atatttcata taccagtctc
      661 tcctatgttt caaggtgtta aaagctcaat gtatatttcc tccatattta agcggcagga
      721 caacggagca gcacatagca tctcactagc agccattgac cttggcctag ccagccttca
      781 ttagctttta gacacttatc agtcataact gggtttgtcc tctccgcaac gtttcctcct
      841 tcacttcact taggctggaa aataatggct gaaatccatt tatctgccat ttccaacaag
      901 tgcgtgacat taacaaatta tctacttttc actaagtatg gccaaggtac cctctaaagg
      961 attcctccac tctgccggcc aaggtcagac ctttttagaa agtgactttt tttttttttt
     1021 ccactacctg agctattttc tctccttccc atctttaaat attcattctg cttttgcatg
     1081 ccgggctgag attttatgag cactatcagc catatttctc caaacacaca tctttatttg
     1141 tttttgatta tttatgctcc actctgactt tataactcac atccctagga aaacattgat
     1201 ccaagaggca aatgaatcta attaggacct ctttcttctg tgctgaatat tgacttgttt
     1261 tgaaaggtgc ctctccattt cgagcgaatg tctctggaat gctcggtccc aatctcttta
     1321 aggaaaactt ctaaatgctg atgagatatt ttagtgcata aatgcaatcc tgtacagatc
     1381 catagtggcg cctggtcgtt cataatgtat tacattaatt gcaggatgtc atcagtgcac
     1441 taatttccag ctctcaggaa ctcaaaggca ccagttgttt ttgcagttct tgagggctta
     1501 agtcttatat ctcaatctta aatttcttaa c
//
LOCUS       KCVB01000270            1347 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12544pri ultra-conserved element locus
            uce-310, sequence.
ACCESSION   KCVB01000270
VERSION     KCVB01000270.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12544pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1347
                     /note="ultra-conserved element locus uce-310"
BASE COUNT          445 a          250 c          260 g          392 t
ORIGIN      
        1 agacagagac ggaagacaga cattaaaggc ccccatctca aacagcagag ggagaattca
       61 taaaaacagc cttcaaaaag cacacacaca cacgcacaaa cgcacacaca cacacacacg
      121 tgcacataca cactcgtact ccatgtgaaa tgaggttgta aggttgtaca caaaagaagc
      181 aagggacaaa agaagcaagc cttagatgta caataatata tgaaaatatt atacattttt
      241 aaatatttct tctttctagt ttgagccaag gaataaaagt gcataggtat gttaactttg
      301 attggacaga agacatgaat ctacaggcag cttttgagat cagaagaaaa ggtttaaatt
      361 aaaaaaaaga gtctttaaac cgtctgccat tgatggcaaa gtgaactctg ggtaataatt
      421 aggccttaga gaaactcgtc atcagcgtcg ctcggcaact gctgctttct ttcagtttta
      481 ataactgaca ctatgagata aaacatcttt ttttttaaag tgccacttta ttacaaaagt
      541 ataaacttga ctgacatttc atgaatagta atcttgatat aaaacattac aatcttttgc
      601 acttagcact taattacacg cttttgagta caagaccctg tagtctgaat agcaaacaca
      661 caacattagc tgccacattt aacttagctg ggctttccaa gaacacaccg tagcttcatg
      721 gcttcaaaaa acaacaaaca gaatacactg aaatttacat tgcagctgtt ttgcagtttg
      781 tgtcacaaga taagtcattc caaaacacaa ttaaacataa tagaaaaaga tagatttctt
      841 gacactgatg atatatttat tatttctttc tcttttgtaa tgtacataca cattgcatca
      901 acatacagta taatcacatt gtttgctggg gatatttggt cagatgaaat catcgttatt
      961 gtccaggatt tggatgtaat ttggagtgtg ttaacctcag ggcgatgctc tgctgcttag
     1021 cgaaccgttt tgcttcacat ttttgtcatt tctgcaatat ttttaacact gataacagag
     1081 agagtgttca tcaatggagt gacaatgaga catgacgttt gctaatacac atcagttcta
     1141 ctgtagcttt ccaagctgca ctatggtgaa acccaagcag attcatatga ccacaaggca
     1201 gggacgatgt agatgaactg tccacagatg tgagtgagga ctgagaggga ggtgggggtg
     1261 tgttttgggt gggtgttgag tggactgtct ccacctttag gactcaagca gttctcttta
     1321 gtcttgattc atctgtactg tgtgtgt
//
LOCUS       KCVB01000271             973 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12582pri ultra-conserved element locus
            uce-478, sequence.
ACCESSION   KCVB01000271
VERSION     KCVB01000271.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 973)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12582pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>973
                     /note="ultra-conserved element locus uce-478"
BASE COUNT          321 a          190 c          208 g          254 t
ORIGIN      
        1 atgagcaata gcatttgagt accccattgc tgtaaataac aaccagaatt accgtcaatg
       61 gcgcaaaaaa ctcccacagg cacactccct caaacacaca caaacacaca cacaggaaat
      121 aaaggaagga aaagaccaaa gatgtgatgc taattgcact gcatttttaa gtaaggcgca
      181 gaaaaccaga attagcttaa tcttctctgc atctatttta caaacttgtt tacctaagtg
      241 attatataca aataatgaac acaaacatac aacattttct tgggtctgtt ttaagagaaa
      301 ggccaacagc ctcaccttgc tttgtgtcgt aaagtcacca ccctttgtac agtgaataaa
      361 tctcgcataa aagcaatatc ccattatatc taacttgctg acacacagtg ttgcacagag
      421 gcccgtagag gcagctgttt atggctactg cactcatgtg ttaaaactaa tgaggtgatt
      481 tattaatgtg aaatttagtg gacatgcagc caaaaaaaag taagcagctg aacgggagaa
      541 atggcaccac cattaacaaa gaagttagga gaatgcacca aagtaattta agtccagtta
      601 attagaggtc ataccagtgg tgccctttgt atgtgtgcaa ggtactgcag caggctctta
      661 gtgtggtaga tggtggggtg gagctcagga ttggggttct gccactggcg gttcaaatcc
      721 tctccactca gagaacaacg atgactacag cagaaaggag atggcagaag aggggaagga
      781 tacagagtgg aggagaaaaa agcaacagct agagaatcct taaggacaag attttgtgtg
      841 tgttaatata aataaagctg tgaccaccgt gcaggcccag gttcagcaga tgcatacatc
      901 ttcagtgtgt atacttgaaa gcttttatgc gtgttctttt ctttctgaag actttttttt
      961 ttttttactc act
//
LOCUS       KCVB01000272            1436 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12624pri ultra-conserved element locus
            uce-368, sequence.
ACCESSION   KCVB01000272
VERSION     KCVB01000272.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12624pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1436
                     /note="ultra-conserved element locus uce-368"
BASE COUNT          399 a          315 c          301 g          421 t
ORIGIN      
        1 atctcttgac aacttagtgt acttcagtta tgtaatgcac tgcagtcaag gcacactgaa
       61 ggaatgaagc acacgataca tgttttggag aaaaggcgtc accacagggt tggctgaatg
      121 aagagatttt atcctaaggc agtcttgcat gtattaatga gattgtagtt gtgaaaaatg
      181 gaatagaaca gatgagaaga ttttactttt tttaatattt tacttttttt atagcctttg
      241 ctgatctgtc ttcacttaaa ctaccaactc ctgtgggcat caattatctt ttcttgttga
      301 taactaatca ttaattatgt aatacactgt agccgtagct tatagaaggg aagtgaagca
      361 gacttcactt tgggcacaat gatagtcaga catattttgc aatcaaatcc aagttaattt
      421 aatgggatat cgttttggca aagcaacagt gtttttggtc ctaaatctaa aagtgctctt
      481 tcagtgtggg ggacatgcag ctctctatgg gttggcatgc atctctgagc agtgggttgc
      541 gggtgagagg tggggggtcc accctggttg gtatatcaca ctgggcccgt cagagaaagc
      601 aggaggggtg cacatttttc accttcccca gaatggcacc ttccatagtg gatcccctgt
      661 ctacccgttc cccaggcccc agataagaag ctcatccgtc atccactggg gccgcattaa
      721 attttaactc tggttaccga gtgtggccat tcaaactttt atctcaaacc ttctttatct
      781 tcttttcatt aagttctcaa gcactaatag ccctgcgagt ctccgccggg gtaaggatga
      841 tgttctgaat aatgaaagct cattatttct ccacagaatg cttgttaatt caaatgatga
      901 tattaaaact atagtagccc tcagcagtcg ggtgatttct agcatacttt atcatgtatc
      961 aatatatact ctgtttgtca aaactgcccc gagcctgggc tgacattctc ctcccgagga
     1021 gtggagtgct tcagccgggc ctgcaatact ctaaggtgac aactggcaga gtttgtgcct
     1081 gtaggaaagt gggggaagga gggatgaagg cagtgatggg gagaaggatg aaactcaaag
     1141 aggagtcatt gtgctcccat gtctattcga cgtatttcca tcatgcacac atgaattccc
     1201 ccctgtcctc ttcccacaca cacacaccca tgcacgaata cactcacatc tgtcatcctc
     1261 atctgtcttg cctgatatgt gcacgtcagc agacatttgc acatattcac ctaaattcat
     1321 acatacacag acaaaagcat gcatgcacat gcacatgaac atgaacattc acatgaacat
     1381 ccatggccat taacctttaa atataacatt ctagcattta atctttaaga catgcc
//
LOCUS       KCVB01000273            1496 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12667pri ultra-conserved element locus
            uce-90, sequence.
ACCESSION   KCVB01000273
VERSION     KCVB01000273.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12667pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1496
                     /note="ultra-conserved element locus uce-90"
BASE COUNT          440 a          271 c          317 g          468 t
ORIGIN      
        1 gcatttggag ctggccggta atgtcatcgt aatttaagcc acagaataat tgagatatat
       61 agcgtgtcta aaagagttcc attaagctgt ttggattttt tttgtgtttt actggagaca
      121 ggggtgtggc actgttgaag tctccttccc gctcttgtac gcccacacac attgcacatc
      181 acaccacaca cattgagtgt acatgcagac attaaatcaa acacactgta cacactgcag
      241 acacacacat gcacacaaaa acacatatgc tgtacaaaca gggtcgactg tcttcagatc
      301 aaggacggga atttgccatt tcagcagtga tttttcaatt tcagtcggtt cagctctgtt
      361 tgattaatat gtaggtgcac ttttacggtt ttgtacattt gaaatagtga aatgcagccg
      421 tccttttact gccattcagc gggtttttct attaaagttg gaaacaaagt aaagtaacaa
      481 ctacagatca ttagatttag acttacctca gagaattagc tcagggtcag atcttgaggt
      541 gtatgaaatt gatatttctc tggcggggat gaatcactgt agtaactcag atattagtcc
      601 attaaagaag aacaataagg catcaatatg catagaatta aattaatggt ggtgagttta
      661 tctctctatc ccagacgcat ccctaaaaga cacagtataa tgtatgacct gaccttcaaa
      721 tgccaggaga gtgttgggca ataaactgcc aatatttgtg gcttaatggc gtcggatgac
      781 ttttactgta attggaatgg cagcgaaaca gctgggctgt ttaaagacag tgcagggctg
      841 aggtctcaga caatcactaa ctcagaatgg tcacagggag tgtgtttaac cctgaaggga
      901 gtttctgtga tagctataag gactaaactg taggtaaaca gaggtaaaaa ggcgcttaaa
      961 ttaccttcaa ttaaaagtta tggtgagtgg gatctgattt tgtttccaga tatctttcac
     1021 tggctaaaag gagaaaaatt gccctttgtc attctccaca ataatacact ttgtactttt
     1081 aaattatggt tccaatacac catttttcaa gtaagaagca aaaataaact cagctacagt
     1141 acatggagcg gttaatcaga actgctaaca tgttggtctt atttttgact aagaaatcca
     1201 tttactggtt ttgatggaat tgggttcttg tctgcgacat ctgccttgtt ctacttgctt
     1261 aaaatttcac tgttttttat ttctggaaga atcttgtttg gtgttaacat ctttgggtgt
     1321 atttattgca catggtggtg gcatccatac tgtaaaatga tggatcactg gagctgaaag
     1381 ctgattacaa gcttgattta ctgcagggca gcgcttaaat cactcaactc agcctggtgt
     1441 gggattgcaa catttttcat gtttagacaa cggttgttgt tagatacttc cctcag
//
LOCUS       KCVB01000274            1508 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12757pri ultra-conserved element locus
            uce-892, sequence.
ACCESSION   KCVB01000274
VERSION     KCVB01000274.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12757pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1508
                     /note="ultra-conserved element locus uce-892"
BASE COUNT          449 a          317 c          292 g          450 t
ORIGIN      
        1 aacaaattat gtgattgtga aactgtcaca aggcttataa tatgtaagct acatcataaa
       61 cccagaggtt tatatagaac taaaatttga gtgtaggccg tttcttaaat taaaaaaaaa
      121 aaaaggatta ggctagttcg ttatgcatat tactcatata tccctaacct cgtaagctta
      181 ttatccgatg ctgaattgta aaaatccacc aaaaaacggt caactggcct ttattttaac
      241 acgactgtga atttgcagcc agctcaatct cgccaccttg aacagagtta gtcgaggctt
      301 tctgcatttt ccaacctcac aaaagtgata atttattaaa cataataaat tatatcacag
      361 ccttagttat gatgaggcgt gctctttata cgtggggcat gaataaacgc tcctttcgaa
      421 aacaaaggct aattgattgc tgttatattg gcatttgtgc agactgtttg cagagtggag
      481 ctcgtacagt agcttatatt tgcctttatt tacagtatgg ttgtattagt gccgacagca
      541 tcagcgcagg gaggctgcct ccagactgtg atggttgtaa aacatagcag gagagaaacg
      601 ttatatttgt gtacatagga ttatcatggc cctgccaggc tactcggctt tagtatcaga
      661 cacactccgt tttgtgcaca ggctgattta gtaaataaga gggcagatag atagcgcaga
      721 tggtttgaag tgtcgggtca gattatttca tggctggata cagtagtaaa gttcattctg
      781 acggaaaagc ctgatattat tttcacagac ccccccaaca cacacacaca cacacacata
      841 cacactctct ctccgctccg tctctctctc tctctctctc taccctgcac gtgcacgcgc
      901 gcacacaccg cacactcgag ctgaatcccc ctcaggctct ttttgcatga attatagact
      961 cttgatgcga ggttatgaaa tgccttctgc acaggaagct gttcattctc ttgctggttt
     1021 actgaagacc acatcatcat atttatggat ttaaaaaaaa tatatttata aacaagaatt
     1081 tgcatatcat ctcttaatgt tgatgggacc tatctgtttg tcagaaatac gcttgcgata
     1141 tcggcggcat aatctttatg aaatcgctaa tttatagcag atagagtgct tggccagatt
     1201 aagacgattc tatctatcgc cattcattca cccacatcat tacacccgcc gttaaatatt
     1261 ggtaaataac ggataagacc tccatcagat gtagctgtgg aagtaaattg cctgggcgac
     1321 tgacaaggtg ctggtctgaa atatttagat tcccgtaaat atttcatccc tgcaggctga
     1381 acgtcccaac ctatttacaa ggactgcaat actttttttc taggaagcgc gcatcaataa
     1441 aatatagttg actgttggat ctcttaaacg gggagctgga aataacaatt gcagactatg
     1501 ctttgtaa
//
LOCUS       KCVB01000275             852 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12792pri ultra-conserved element locus
            uce-28, sequence.
ACCESSION   KCVB01000275
VERSION     KCVB01000275.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12792pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>852
                     /note="ultra-conserved element locus uce-28"
BASE COUNT          274 a          151 c          172 g          255 t
ORIGIN      
        1 tatctttttt ttttttttga actgttgaaa ttgtgacaaa cttgatgagt aaggagatga
       61 aggaggaatt gaaaaactga atgcgaaact aagccaagat atattcacaa gatcctctaa
      121 gctgcactgg tttacaggtc aacagatgag agctctagcc ctagacttac caatttcacc
      181 ctcatgcata ctgtacggtt aaagtgacgc aacaagcttg cttaaatagt ctacatcaca
      241 aaagcaattt ggttttagtg caaacacaat tggctggctt cagcttctca ataataacaa
      301 gctcaccaag gagattacaa gaaggcacat acttatgctt cctctaacac gcaacatatt
      361 tagacaaacc gcaacatctt tcaacaacac cacaaagcgg tgaaaacagg aaagctacta
      421 catccagata aaatggtgaa gttttgcctt ttgtgaatta ttgaaaaatt tacacttatc
      481 catcaacaaa ataacgacgt ttaggtcaat agttactgct gctgtgaatg acctcgttga
      541 gaaaggatga agtatgatac attttggtgg acacagattc tctacatatg tggccaatga
      601 tgctgtgttt gtatgacgaa aaggtgcatt tgaaaatttt ttaaaatttt attattagtt
      661 cactttaaag catttttttt cttttttgtg acgtcccagc gagaagcact caaagtgacc
      721 aaatatgcag agaagacgag tgtgttttta agggagaagg tgtgcacgac agtgggcagc
      781 tgggggttat cttttggtat ttgtgttcgt ctttaataat gtcgtcggcc gtgtgatgaa
      841 gacaatcgac ac
//
LOCUS       KCVB01000276            1932 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12834pri ultra-conserved element locus
            uce-7, sequence.
ACCESSION   KCVB01000276
VERSION     KCVB01000276.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12834pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1932
                     /note="ultra-conserved element locus uce-7"
BASE COUNT          614 a          433 c          396 g          489 t
ORIGIN      
        1 agatgccaat ccatgagatt aaagggagag gacgtctttc caacatggtc caaagatgtg
       61 aaaagacata aaacacaaca tgaaataaaa taatagtctt aaaacccaaa cttcaccggt
      121 gcaaaatgaa ccaaagtcaa acaaaaacag atgaatagct cctgtcatct tactccatga
      181 ataagctcct acatgactcc tgaacgtaaa atcttaaata tttgttaatt tcaaatattt
      241 aatgatttga ccatccgtac atttctccga cagatcaggt caacatagtc aaaatctggc
      301 actttcccac tcttgcagat tgaatatcaa atgaactgta gatacattga tcacatcgcg
      361 ggctcctcgg ctctcacatg tttgtctttg cagtgtgaac agactaagcc cctctactca
      421 gctgtcataa tttgttaacg agtcttttca gggagggaaa aaagaaaaag agaagacaga
      481 aaaaaagaaa agaaggctct tcacgagtga ctgaaagcag aggtccatcc agagagggaa
      541 cccaggggcg acatcagacc gatttcagac cagcccgtga tggcatctct gtatatttta
      601 atattaatga ccccggccgc aaagccgcct ctaagcctcc aacaggtgcc ccagccccac
      661 tcgagtctga aaacccgaga tcattcaacg tcccaccatc caacaccacc accgggcaat
      721 aaagactgca aatgaggagg atgaaggcaa agaggagaaa aaaagacata aagagtggaa
      781 gaggagttag agagtcaggc atctccggca aataagtttt tgagtcactt tccccagctg
      841 aaacaccggt cgatatttcc ccccctccct tgagaaagag attgactttt gtttggagtt
      901 tgtgagaagt gggaatcaga agcgggacaa tgggactccc cacggtgaga ggctgagagg
      961 agccatctca aatcagtcag ccagaggact gactaatccg tccattgtcc ctccccagcc
     1021 accggccgca catcaacaca ttcgcccccc attgtccccc accctcatgc agcgggattt
     1081 gctctcccca acagccagtt ttgtccaagg cagttcgagc ggaagtttct cactgacaat
     1141 ttgccccaaa tagatttgtc agagcgtcca actgcaccgc accgacttgt gggtttctgg
     1201 gtttgggttc tgggtctggg ttttttgggg ggactcttgc taggcgaggc cttggcgtgt
     1261 gtgtaaaagt gtaagtgtgc acaagtttgc atgtgtgcat gcagaggtgg aggggatggt
     1321 gagactgtat gaggtcagtg aggaatctca ccttaggaaa tcaagtgcta ctggttcact
     1381 gtaactgctg acagacaacc taagactttt cctgtttttt gataattaaa ctcctgtatg
     1441 gaatcaacac tgacagagag aaacatccaa caccctgaag ccaccaacaa aaagtattca
     1501 gatcatttac ttcaataaaa gcaccaacat cccactgtaa aatacttgta ctccattaca
     1561 agtgaaagtc ctgcattcaa aatgtgactt atgtaaaagt gtgtaagtac cattgcactt
     1621 aaaatgcact aaaagtatca aaagttaaag tactgaattc actaaaatat cccctttgac
     1681 tgttatttat catatcatta gattagaatt gctcatacat gaatgtgaga gcaacatatt
     1741 actgttgtag ttggttaaac tgattatttt tattgtagat gaatctactg attgttttct
     1801 acattaactg attgattatt tggtttgtaa aaaaaaatta aaaaacagtg agaaatgcca
     1861 ccactatttt tcagagccca aagcgacacc ttcgctatgc atgttaagtc catccaatag
     1921 tacaaacctc ag
//
LOCUS       KCVB01000277            1086 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12876pri ultra-conserved element locus
            uce-395, sequence.
ACCESSION   KCVB01000277
VERSION     KCVB01000277.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12876pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1086
                     /note="ultra-conserved element locus uce-395"
BASE COUNT          345 a          223 c          218 g          300 t
ORIGIN      
        1 gtattttatg gctccaatta actgaaaaat atggcagtga gcattaaatc caggcttcag
       61 gagtctctta gtgatgtcaa acatgcattc aggtcattta tactatatgg acttgagata
      121 tgttgaattt agactttctt gttaagagtt attgtgtggt tctgacacat ctaaactcat
      181 ttctgatccc aatcagacca accgagactg catttttaat tattttgatc actaggactc
      241 cctgttaagt ggtggctggc atacctgcgg tcaggaagga agtacatttt gacgtagggg
      301 ctcctgggcc gcccatcagg ccgaagagga agctccgcag cctgcagcac gttgactatt
      361 agctgatggc ccactttgtc gtaccacagc ttcaactggg ggggaaacac acacaaacaa
      421 atctgatgct ggaccttcac tctaactaat catcagccca acatacataa agtgcacaca
      481 ctcactgtga gttgccctgg cattaactgt ggagcatctt ggagcatccc gggactggag
      541 ggagacagga catttaaaga tggtctctcc atcttcgggg actcaaaaga actggaacct
      601 gctatgaaga caaaagaagt gacaaaaatt caagtctaga cacgagtcag agggaaagta
      661 tgtgaaaatt aatggggttt gtatagagtg agagcataat caggttaaac tctaaaacag
      721 aagtgtgttc atattacata atatccagca ttaaaagttc ctttctttgg aaatattcgc
      781 ataaaaagga aacacaccat ttcaaagcta accagcgagg atgacaaatt accaagcttc
      841 tgacctactt taaggctttt aattcaagta ttggagcctc atataccctt caaaatggtc
      901 tcatgatcaa gtatcatttt aaccactgac taatgcataa ccatatttcc ttacttttgc
      961 acacatcgta gatgtcagat gtatgaattt tttgtaaagt tagcggttag caaccttgcg
     1021 acctcatgac cttaaaagtc atgaggtatt aataataaat aacccagcaa cttatcaaac
     1081 aaaaac
//
LOCUS       KCVB01000278             996 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12917pri ultra-conserved element locus
            uce-744, sequence.
ACCESSION   KCVB01000278
VERSION     KCVB01000278.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 996)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12917pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>996
                     /note="ultra-conserved element locus uce-744"
BASE COUNT          281 a          231 c          236 g          248 t
ORIGIN      
        1 gtacagccgg tacgggaagt ttcatacggc ttggaccctg ctagtaaatg gcaccacaga
       61 agcaattatg catcgtgcta ctcgggagag gatctggtgc atagggactg tcttccacca
      121 tccaccatga cagaaatgct tatgaagaac gagagtgtgt acagccacca ccatcaccat
      181 cacacccatc cgggctccaa tcacccctcc acaggttttt actccggcgt ggggaaaaac
      241 aacgtcctcc cccaaggttt cgaccgcttt tttgagaccg catactgcaa cagcacggac
      301 aatcagtcag acaattgttt acaaaagggc gagggaggaa agctggaaag cgactcccag
      361 ccgcagcagc aacaacggca gcagcccaca gcctctgtac ccggagcccc ggagcaggaa
      421 aaagacccag aagatgagga ggaacataca aattcaggct catgtacttc ttcctccgca
      481 acaaccaagg acggcaacgc caacaagagc agccactcga gtaggtatac cgaagctgta
      541 aaatggggga aggttaaggg gactagcccg gtgttttgtc ggtcagattt tatgtggagt
      601 tttataagca ttcaaaggat tttattataa ccctacaaag ctctttcttg caacgagaag
      661 aatttttacg atgggaaagc gctttgaaaa aacggggatg ttattcacag acgcctctat
      721 ggtgagagtc tgtgaaattt ttaagagagc agctattgtg acaaatgtta actttgatca
      781 tgaacttgcg ttgggcattt taagggattt tctcgttggg ctgtcgagag taaaaaccaa
      841 ttgggcagaa cattgctttt ggcgtcttgt gaattttctt ttagcaccga gttagcccgc
      901 aaaagacatg ctgcaaagag tattagattc tttccggttt aatgcgtttc tatctgtcta
      961 tctgtctatc tgtctatcta tctatctatc tatcta
//
LOCUS       KCVB01000279            1162 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12956pri ultra-conserved element locus
            uce-701, sequence.
ACCESSION   KCVB01000279
VERSION     KCVB01000279.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12956pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1162
                     /note="ultra-conserved element locus uce-701"
BASE COUNT          261 a          233 c          267 g          401 t
ORIGIN      
        1 ttttttttac aaaagaaggc ctgttgtcta aatgaaagct tgctgcagga ggagttttct
       61 ttctgtggtg tggaggctgg acttattatg gtatgggagc ttttgccttc ttttgtccgc
      121 tctctctaca ctgtgtgttt tgcactgtag ctcagctcct tggtcttgtc tttccgtctc
      181 tgcctgtatt tgcttctggt ggctggggag tgagtgtggg attgcatctg cttcaaggtg
      241 gtccatccca gtggactagg aagtcccgca acctgcaagc agtgaggagt gttgggtgtg
      301 tttggtgaag tttatggaaa gtgtgagaat gtccttcttt tgttttattt ttttttaagc
      361 gtgtgtgagc atatttttat tttggcctga ctaatccgca tgcctctcgc tggttcctgt
      421 cctaacaggg ggtgtcattt gatcaagtca ataatctgct gattgaacct gctcgaattg
      481 aggaggaaga ggtctgtaca tactctttcc cgtcctgtct gtctatctgt gtccttcact
      541 cacccactca tttacccatc tgtgttcaga gaaagtacca atggttaatc caagccaatc
      601 accagggacc cccttaacag agctcattta tgacaaggcc agtgtggcag aactggatgc
      661 aggaatgaag tagccctgtt ttatttattc cgtttattgg gttttgacca gacagcaggg
      721 tttaaacttg cagtgaggtt ttgcaaatgt gctgtgtata attgaatcct gggaaatttg
      781 actagttttg cggacattga aaaattgtta ccattatata tggctaacca tttttttttt
      841 ccggttaaaa ctcgttctga gacaaatctt ttagacagcg gctccgtggc tcaaaagggt
      901 aagatgctga acaggaagta attgtggaac cactaagatt gtattagtgc atctttgggt
      961 tgtccatcct ttctgtgtgg gggttctcag ccataggata acttttccat tcataatgtg
     1021 cgtgccattc ataccaccag caacattgtt taaagccaga aatgcagctc ttcctcttac
     1081 aagttcacca cgccagctat tgcttttatt ggttggtctt tgcaaaatta accatttcta
     1141 aaactgattc accatttttc ta
//
LOCUS       KCVB01000280            1596 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 12994pri ultra-conserved element locus
            uce-131, sequence.
ACCESSION   KCVB01000280
VERSION     KCVB01000280.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="12994pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1596
                     /note="ultra-conserved element locus uce-131"
BASE COUNT          485 a          275 c          327 g          509 t
ORIGIN      
        1 tttttttttt gtgttcagag tgtcaaggaa ttactagcta gacgtgaacg tagtcttaaa
       61 gaaagcaccc agttctctgt gatagtataa gtgtctttgc tcttttctgc aagcgattct
      121 aagagatggc cttgaattga agaggtcaat tcagctctct ttgttagtgc cgagactact
      181 cacggatgca aaaggctcgt acgccacctt tgcgactgcc tgagctttaa ctaaatgaca
      241 cagttagtaa aacctctgaa taaccaactg gaaaatataa tttaataaca gactttcggt
      301 attcatgaat cagtgaaagt gctcagtgac agcatgctct aatgttcaca ggtccgatga
      361 gcataaatct ctctgaggag aaaaggtgtg tattacgtac tttgatatca tcaggtgaac
      421 aagagggatg aaatgagctg ctagcttgag ttgaatagca ttcattttgc tcacaatggc
      481 caccctccct aatacctgca gtgttttact tagattagac ttaagctttt tttaaaggcc
      541 atttcaataa tttgacattt ctccccagaa tgggagagaa tgagcacaga taaaaacttt
      601 tttttttctc cttttcatgc aaaacacttt catctgtcat tttgtgatag cccgtctctc
      661 tttgtcagca taaatacggg agcccctatt tgaatatggt tacacatccc tctatttatt
      721 aaagcttgga ttaacatgac aggaagtttt gtctgggggc tctttaagat ggctcagaga
      781 ggcaatctgg tgacagtaat aggatccagc taatatactt gactgatgct cctttcccag
      841 tgttataaag cagaacaatg ggagaggggt tctgggagtg aatacattgt gttttctaat
      901 agtgtgtgtg tgtgtgtttg tgtgtacgtg tgcatgtcta gggggacgag ggtgcgctaa
      961 tcagccagaa taccatcact tagcaaatat aagggctgaa gaaataaaaa tactaaaggt
     1021 cacagagcaa ttccatcagg acaactatgc tacaattaac tcaatagaca aaggctctga
     1081 cattagtgag agctataata atcttttact ccagtgtgtg agtttgtaga ggtttgttaa
     1141 aggttggctt taagagataa acagaaattc ttttcattat tgttcgtctt ccaccagtga
     1201 aatccttctt gagttatgtt ttactctgat gtaagttgtt cagagattac tgatcccaac
     1261 atgtacatgc gttgttctct acgtattaag gatattttag taaggacatt tttactcttt
     1321 cattttttgt ttgtatagac aagaatctgt ttgtgaaacc tacaacatat ctatcctaaa
     1381 aaaacatttt attataaaaa ctaatagcga aaacatagtt tttcaaaaat tttttacagt
     1441 gacatattgc tttaagtcaa aatgaaaaca aaaaattcag tgtggcagag tcacagagag
     1501 agtaaaagag aaagacgttt agacacgtga gtaatgttag tgttacgtca aaggccactg
     1561 cgccaaagtg ctgcatgtgt gcgctacata gtgtaa
//
LOCUS       KCVB01000281            1309 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13035pri ultra-conserved element locus
            uce-972, sequence.
ACCESSION   KCVB01000281
VERSION     KCVB01000281.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13035pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1309
                     /note="ultra-conserved element locus uce-972"
BASE COUNT          410 a          262 c          293 g          344 t
ORIGIN      
        1 aagtgcattc taccaatgac agtccaacaa acatattcag acatccaaca gagatgagtt
       61 tatagtgttt aatcaaagcc tgaagaggaa ctttaatacc ggcgttttgc aatctctgca
      121 tcccacactg gtaacaagaa atagtcacaa gaataaggac acaaataaaa ctgcaagtaa
      181 tttaacatgg ttcaatgttc catattaaag gacatttcta tacttactac tataaaagac
      241 acaggacaca tgagtgaaca gagaattatg tctcttgata atattgttta tcactgaaat
      301 atcaagagac ataattctct tgagaaaatt gtttcctgct gccaaagaat tgcacctggc
      361 tgtacagtga aagtcttttt gtgcacagtt tacagaagta atgtaaacat ccgcttgccc
      421 ttatgtttcc tcctctttct ttatccagtc gtacacacac aatcacacac acacaccctc
      481 tcccaacaca caaattttta taggaagctt gaaccacagc acaaggttaa tcaatgtggt
      541 agctggccat aaatttggtt caagtgacag gagaatgtcc atacctgtct cagctgctgg
      601 tgatctgtga ttggtgacag caggcccgac tccctggctg gtgctgaatc atcaggagca
      661 gctgatttag ttacggcagc tccgacaggg ccaatctatc aattgatctg gggagtaatg
      721 cattaccagc ttgggtcaag ggagcctggg cagaatatag ccatagtcag aaaccacagc
      781 gggtaaaggg ggcaactcgg caggcctgtg agcagcagag agaaggattt agggagtgtg
      841 atggcgaaca cactaatctc actgttagct gaatgtggca cataaaacta aacaggcgtt
      901 gtgaggtagg atgatcatta gggagagact gatggccttc ttgctcacac agctcctctg
      961 ggagttgcta tgaagaatgc tcaatcaaca atttggctga ctgatcacat gcagtaaaat
     1021 gtgtgtgttt ctctgtgctt gtgccgtcca tatcagattg tatcctcttc tttcttcaac
     1081 tgccattgtc ttaaaagtaa aaatgaaaat gttaggacag agaataaaaa gagtcattgc
     1141 gaggaagaca aagctgctga aaattgagca ttgacaggtg cagggaaggg aaggtgtgag
     1201 cagtgtttaa tagtgagccc tgggctaaag gccattatac tgtatttcta aaaaaagggg
     1261 ctgtttggac agtctaagag caatcagtcc ctctcaaaca gttggataa
//
LOCUS       KCVB01000282            1387 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13072pri ultra-conserved element locus
            uce-174, sequence.
ACCESSION   KCVB01000282
VERSION     KCVB01000282.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13072pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1387
                     /note="ultra-conserved element locus uce-174"
BASE COUNT          399 a          316 c          316 g          356 t
ORIGIN      
        1 gatatccaac ttatctgcat atgagttggg ttgcaaagct gcaaggggta agagacagag
       61 aagttctgcc actatctaga aaagacaaga gccctgtgat cacttctcct ggtataaagg
      121 agctcagccc tgtttggcct ccggtggagg tgatgaccta acaacaagga cgcagcacca
      181 aatgagacaa catggcctga gaaaagatag agagtaaaag ccacacagag atgcatagcc
      241 cctgaaagca cagtgaacag tattgataat gcagtcaaca acagaaacgc agctgacaga
      301 ctgggagtca aagtttgggg tcaaagagtt gggcatgaac ttcatctttt agtgtgtagc
      361 ttatcaaatt agactactct gttctgcaag tttattaaga caattttaga cacaaacttc
      421 aatgggattt tatcagaaaa gtttgttcgt ggctggtaaa ggctgcaggt acagctgagg
      481 cgcctgaatg agtccaggaa gcgatagggt gggagtcttc cattctcatg gggccatgga
      541 ggtccatcca gaccagtagc tggtgcagtc tcctcctttg tcttcccttc catctcagca
      601 gagaatagca aaagagaaga ggggaagaag ggaagcccct tacgttcatg tgttttgatt
      661 agcaggtaat gatctttgat ctaatgcgct gactgggaaa gtgccgctcc cattgaaatc
      721 cggtggatgc ttggccgacc tggagccagt gtcacttctc gttagggcct gcagctccat
      781 gacctcccca ttgccagtcc cagccccctc ctcgcagcat gtgcaccccg cgtaatgata
      841 tcaatagcag caggggtgca atgaatacaa atccgcgtgc agaatgtttg gacaagaatt
      901 cgctcccagc cctccctcct cgtcctgccg ccccgctcac caccgttcaa cctctatccc
      961 ccacacaaag ggctaggtta ggctgtaatc tggatggttg atttcactca aaatagttgg
     1021 ctactgcaaa gaacaaatgc cccctgctgg ctggctataa ctgcatatgt ttcataaagt
     1081 actagttagt ctgttttcat atcccctcca tcaccgtgag ccatactgga ctataccagc
     1141 atgtgaaaat gtgtgcgatt gtattgaatt gtttaaattt attcatgcat gcggctaaaa
     1201 tcattcaagc tgcaaagcat atgataatgg ctgctacatg cataattgaa agaagacaaa
     1261 actgacttgt ctgtttgcat gaaatagggc agttcacaaa aatctttaat taaaaatatt
     1321 gtttgtttca cattatctca aattaggcac actttcccca tgatgtcaag taaaacaaaa
     1381 attatgt
//
LOCUS       KCVB01000283            1656 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13105pri ultra-conserved element locus
            uce-937, sequence.
ACCESSION   KCVB01000283
VERSION     KCVB01000283.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13105pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1656
                     /note="ultra-conserved element locus uce-937"
BASE COUNT          476 a          394 c          349 g          437 t
ORIGIN      
        1 tctctctgga cacacacaca tatacacaca caccgtctgg ggactcaaat ctcagagcaa
       61 ttttcaggcc ggatgattgt cagcttgatg cgtattcttc ccctacaaga ccggctgata
      121 actccagcta tcccattcca tgccaccatc catccctctt ttctccctct ttctcccact
      181 ctcaccatct tttttttttt gtcgctgtga ctctctcaat agaccctctc cttctcccct
      241 ctctcatccc tctttccatc cttctttccc cctctctgtc tctctctctc tgggcctgtt
      301 gtgcagctat tacaatgaca gaggcgataa gggacattta tcacatgtgc ggcgaggtga
      361 gcgagagaaa gccctcaaca ggtctagagg gaggcacaca caaacccaca cacacatgtc
      421 caaacacaca cacattccgg attgagacat acagagaagc acacactctg catgcatgca
      481 aatgtgcagg cacctgtgca catttatgca cataaacaca aataatcagt caaaggttgc
      541 acatgcttgc atacaacgtg cataaacttg catatccaca cacacacgca cacacacaca
      601 cacacacaca cagagtgaaa tggtgaatca tcccattttg tattttgcag ttgactgagg
      661 ccaattcccc ataattcaat acagttgaaa aatgaaaccc cggtaaatcg ctacagcgat
      721 tttacagatc cccgcttatc ctcataattc attatttcca aactgtaaca acattacatt
      781 tcatttgtaa tgctccgcct gcctcagcca tttgccaatt gttatatctt atttttttcc
      841 ctctccaagg gggagaaaag tgagttattg aggggagaat ggcggctgac tgggtggcac
      901 ggtttgctcc ttcgctgtat atctacctgt ctgtctgact cccattgctg tctatttatc
      961 gttcaggctg tctccggatc tgccctgtct tcctctacat ctgtctgtct ggctgactga
     1021 ctgtctctct aactaactgt agtgaaaacg cacaggcagc gccaaagtaa acgaatgcaa
     1081 gaaacagtat aagttgttgt tttcattgtg ggcaattgat aatgaaccta ttgtttgcag
     1141 ccttagacac acacgcacac acactctacc gtagactgac tggactattg tttttcagca
     1201 ccgtggtaca ggcagatcca cagatggatc ggtgcagggc agagacgggg acaagggtgg
     1261 ggtgaggatg aggaggaggt aaggagtaag aatggaaaga tgggacagag ggaggagagc
     1321 gggtgcatgc tggtggacag gaaagaggac ttatgtgaag agagagggat gatggaagga
     1381 gagatggtga gagtggaatg aataaatgaa atatgggaaa acagagacag gaaactatga
     1441 cgacaggcat tagtgaagta ttgagatctt ttacttatgt acaggtacaa atacctcaat
     1501 gcaaaaaaat ctcattgcaa gtaaaagtcc tgctttcaga attttgcttt agcataagta
     1561 cagaactaat aacagctaat tataatttaa gtatcaaaag taaaagaact catgatgctg
     1621 actagttcct ggcagaggcc tttataattg gatgaa
//
LOCUS       KCVB01000284            1555 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13138pri ultra-conserved element locus
            uce-659, sequence.
ACCESSION   KCVB01000284
VERSION     KCVB01000284.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13138pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1555
                     /note="ultra-conserved element locus uce-659"
BASE COUNT          491 a          304 c          385 g          375 t
ORIGIN      
        1 ctcattaggt ccacgaagtt cagcttctca cacgtttagg tcatgcaact gatcggagag
       61 ggtaagaaac aaaacactta tttggagata taacagcttt ttcaaaccta ttctgatgag
      121 ctgtaggtca ttttcattta agctaataaa tagaaactgg caaaaacaac ggtacttgaa
      181 ttaagaatgc cataatagtt ggtgaaggaa atctggagtc tggggcagag agtttaagca
      241 tttgcacaat ttactattgt ttggtgatcc aaaaagacat gaacaacgct taacaaagag
      301 agattaaaag aggaaagtga agacaagatc aaagatcgcg gcctggagaa agagaaataa
      361 acaaaactga gatgtaacag atttactggt aaaatgtaaa gaaaaaaaag gcaaataaga
      421 gaaggctata aggctagtgt gtttgtgtgt gtgtgcatgt gtgtgtgcag gagcgtacat
      481 gctacggaag acacagcaag catcagtaag taggagagac aggaggtggt taggcggaga
      541 acgagtgaat gaaaagaaga tgtgtggggg agaaagaaaa agtgagcagg aggtggtggg
      601 tttggtgggc ggggagagag ggttgtaaga aaaaagggag aaagagagag agggagagag
      661 ggtgaggaag gcagggagac aggaaagacg gccccctagc ttcttccttt atctccttcc
      721 tgcatggtga gatgtgtgtc ctagatgcca gtatcagtct ctcctgatga aacagatgtg
      781 ctgtcagctt caaacggcac gctaacctgt catatcataa tctaagtgga cataatacct
      841 gatatatata cactggcacc atttactgct gcctgagagg agatgggtgg aggaggtggg
      901 ggaggtgagc agctgcacca tcgactgtgg ctgtgagatg atgtgcatgt gtgtgcgtgt
      961 gagtgcctta gtaagtgcct gtttgccaac gcgtttgtcc gcatgctcac tttcctatgc
     1021 ctatgcgtgt actttacgca ctctgcagct tatacatgtt tgctcttaca gtgcaaacct
     1081 gaacccaccg accataatat tgcctataag tctgagttca ctgtgtgcta ctgatacacc
     1141 cttctccaaa tgagcaacat actcccctaa cgcttgacag gatttcagtg aaatgagagc
     1201 actacaggag aacctggctg cgcttcaatc ccactgacag actgagaccg agacagtttc
     1261 taaaactcct gaaaatccct aacacagcag attagcatta tatgctgtac tctctacaac
     1321 acatctgcag agtgtcttct gttacagtgc cttccccctc gctaacctgc ttccagaaca
     1381 ggaggctatc aatgcatatc agccatttca tataaaaaaa cgcatcctgc agaacggcta
     1441 tccattcttt ataggcccac acaaacacac aaaatagtag gaattaccta tgtgagtcac
     1501 tctcatcggc agcaaaaaaa tagctaaacc ccaataattg gcagtgctta ataca
//
LOCUS       KCVB01000285            1739 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13168pri ultra-conserved element locus
            uce-288, sequence.
ACCESSION   KCVB01000285
VERSION     KCVB01000285.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13168pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1739
                     /note="ultra-conserved element locus uce-288"
BASE COUNT          474 a          431 c          296 g          538 t
ORIGIN      
        1 aaagttttat taaaatgaac ataaatgacc tccgtttgtc ttgcatcaat tgataataat
       61 gaaaaaccct ttggctgtac tcaaatactt ctattgccca tcatttttat gacacaggtt
      121 gtttttcaac ctgtaaaaac atcagacata aatctaaaag tcaatccacc ttcacgccac
      181 cggcccttat ttggctcaga acgcgtctcc attgtgttga cagaaatcaa agctggcagt
      241 ggagagagaa atagtgaata tgtatacctt taatccccct tatcctgctg ttagaccagg
      301 gtgtgtcccc cagtcttcta taatagtctg caatgagaag cttgagtaaa cacaatcaat
      361 ggaagacact agcatatgca ccacctagac ttccgaagtc tccccctttt tatccacccc
      421 tccgcgtatt cgattacaca cccctacctt tgtgggttcc aggcaaatta caacctgaga
      481 caaaggtatt gatgcaatat tacatgtggg tagcaataat cactgatgtt atcaccgtca
      541 caggattgcc ttggaagaaa aaacctgatt ctgccagagg ggtgccctct ctccctgtct
      601 ctctttcttc catgctctct tactcctgga ttacaatctc ttcatccatt attaatgaag
      661 ttaaactact agtggcatgc tgaatgtgct gcatgggaga tttgtagcta atccagcgca
      721 aaacagcccc agtcacataa tgaagggaaa aaaagcacaa atgtcaacag tttcacactg
      781 ggactggatt agacaccccc aactcaccct cctcatccac acacggcctc gctctcgttc
      841 tttctcccct ctacgccagc cactcacaca cacacacaca cacacacacc acaagagtta
      901 tgagtataca aacatctacc tccctccatc accttttctg tgatgtttcc ccactaacgg
      961 attagtgaat gtgaagtact gatttgaagg ccaaagggct gttgaggtct atatgcgaag
     1021 ccgctcaatg caatcttcca aagattaggt gcaaggctgt ttcttaaaat cagccacata
     1081 taagggatgc aagacaaaga tatacaaggc atgcttgcag taagagtgga tgttgaccct
     1141 gcatcgtctt taaggttctc atgtgtcact tgtcatctac agattgcatg aggttggtcg
     1201 ttgtgttgtg gtgaaacact gcactcatgt acagttcagc aatgacatgt ggctccatga
     1261 aaagaagaga cgagcatgaa acactggaac taatcagaac acaggacatg ggactagcat
     1321 cctgatttta gattagccac cctccctgcc tccctccatt tctccctctc tcactctctc
     1381 tttctctctc ctcatatttt ttatctttgt catcctttat ctaccttttg gaggtggtct
     1441 ggtttgattt gcatcttgct gtttttaaat ctgttgtgtt tcctccttta ttttaccctt
     1501 taatttttta aatactcatc tctttttcta tcttctttct tctgtctgtt tctctcatcc
     1561 ttctgaaatt ctgctccttt atcaaaactt ggctgttcat gctagtttta ccttgttgtt
     1621 ttctttaggt ttgcagtccc ccactgaaac atcaaaaagg cagaaaaaga ctaaaaaccc
     1681 cattagcttt aaaagcttga taatcagaat taattgcatg ttggtttcac ttcataagt
//
LOCUS       KCVB01000286            1571 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13212pri ultra-conserved element locus
            uce-1146, sequence.
ACCESSION   KCVB01000286
VERSION     KCVB01000286.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13212pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1571
                     /note="ultra-conserved element locus uce-1146"
BASE COUNT          481 a          320 c          311 g          459 t
ORIGIN      
        1 gaccaataaa taccaaggat ttattgcgga gtggggactg ttgaggcaac cacatgacaa
       61 gaatcaaaga gaggaaaaca tcacacagac tgttgtcata acaacatatt gcaatgaaca
      121 caaagtacca tctggcatga gatttttaag acacatcgag aggacaagcc gatagataat
      181 tatttccctg gaataaaaat tagactgaat gagcagcttc catataaaca cttttaaatg
      241 ttgtgcgcat ggaaagaaat tcagcatcaa agcagtcaga aggcacaaag gagaaaatgc
      301 tgcaagtttt gggaaagtac gatttactct ctactttaca agctctactt tataattgga
      361 ctggaaagta ccagttagga gagaagggga aaaagttttg attgcacaat gctctttgag
      421 ccaaatgtcc taattcagag ttgttaccag ctcacacaaa cattaatcac acacattaat
      481 ctgtattcag tccaggtcct gcctgacctt gagcttttca cctgaggtat gcgctttgca
      541 tccattaagt cgacgtggtg taaaaccatc tcacagcatc agcgtgaagt gtctattacc
      601 accgtcttat ttcctgttta tatggcaact gcctcaacat cctgttttta attacttctc
      661 tccagcagca gatgcttctc acagcaacct gctgtggatt actcccctga gaactaatgt
      721 gctgtgacag accccacttt tccaatcaat aactcgccct aaatctgcca tctctctgcc
      781 tgccaaacac actcccactt ctaatggcac atcattttct ttaagggggt gtaattgcag
      841 actgcggaaa gctgtagccc tttgtgtttc cactatgtag atactgtaaa ggaatgtaaa
      901 gtacagaaac cacagcacac ggtcgacaca ggaagtgatg ggggctttac actgtgctca
      961 gtctcgccat tgccatcaca ttagcccttg agaaagcatt cacttcccta ttttggctga
     1021 gtgctcagat aaatcgctgt gctgctttat aaacctgttg gtgcaatctt gtcaggatgt
     1081 ttgtgtgact tctgtggtaa gtgcagtttt tcattgttca ggcaaggcta gctgatctgt
     1141 ttgaccttta agacggaggg ggaagagcaa ttttggcaga catatttacg actcatttgc
     1201 catatctgtg ttttggtggt gagtctgggg acaagctaaa gtcaatcacc caacttattt
     1261 gttttattca tcttcagctt cacgttacat gatttcctga ttttaaatga ttacccacaa
     1321 ttcacaacac acagaacaga cagtacagag gaaaaagtga gtacttaaga aggaaagcag
     1381 gaagtcacat gatttactat caaaacagca ttaatttcac aaagataaaa aaattcactt
     1441 tcacattgtt cttaatatga aagttaactg cattatattc tccattcttt cataaagtgt
     1501 gtaatctgtg tgaatatact gtatatatgg aagacatgag aatgttatgt gtaaagtgtg
     1561 atgttaagtg t
//
LOCUS       KCVB01000287            1601 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13293pri ultra-conserved element locus
            uce-230, sequence.
ACCESSION   KCVB01000287
VERSION     KCVB01000287.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13293pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1601
                     /note="ultra-conserved element locus uce-230"
BASE COUNT          457 a          324 c          345 g          475 t
ORIGIN      
        1 acaaaactgg tgctgcattt gaaagggtca gcacagctaa cctgagctat tgtcttaata
       61 cagatttaaa cgataaaact attggacagc aaattaccga agcaatagaa attattgaat
      121 ggaatttaaa gatggagatg tagatgcttt gtcagacaaa ggaaccagcc aaccaagctg
      181 gcaaagacag taatggttaa tgtggttaat gttaactaac taaccagatc caacatgtta
      241 ataacaggac atgatggtta ccagatcaga tgctcatgtc ggcaagttgc ttttgaagct
      301 ttcacaaaag cgcaacaagc tgttagctaa aggacacaaa gttttatagt aactgggaca
      361 cccaagtgta attttagcca ccttacacag caacggttta gcagcaacag gtgtagaaaa
      421 agccgtggag gcgtaggtat aaatctgaga tatttcacag atggagatca ctgtaatagt
      481 ataggtagga catgaagtca ttcacactac ccggctcagg tgatcttcct gtagagtttt
      541 gtttttcatg tcctagtatg agacaaaaac tcagacaggg atatttgtgg ctcaatcatt
      601 gttgccagag aaatgaggat ctccaatata gctgccaaaa agtgtcacac atcacacgaa
      661 agcatcccac tgttgtacat gttgtctatc tactctgtgc ctgtgtggag attatgatct
      721 ttgtatttca ctttcacttg tctttctttc ttttctttct ttttgtcctc accctctctc
      781 tctagttgtg tgtgagaata gagaagaacc ctggtcttgg tttcagtatc tcgggtggca
      841 tcagcggcca ggggaacccc tttaaaccct ccgacatggt acgacctgac ccggcctgcc
      901 agccaatcac agcctcccca gctttatctc agagttgcca tggaaactgc tgctcgttac
      961 tgcagtgttt cgtctatctg tccgttcgtc tgtctgtctg tggggcagca gtggacttgg
     1021 ttaactttga ggctgttgtc ttatactaca gaatgttggt ttaacagcag tttaatgaaa
     1081 ttaatttatt taaaagggta aaggagcctg aggtgtgata aaatatatca aaactaaacc
     1141 tcttgaaaca tggaaatcct tgttagcatc accaaagagg attgaaaact tcagagaaat
     1201 gatatctgag tttgtcaaag tacataatta ggactgatag tacgcactat gcatgtacat
     1261 atcattaata gtgtgcttaa tgtgtttggt tcattcttac ggtgtcctca tcacatcctc
     1321 aggtctcatg gaaaattaag ttacagcaac aaacttgaat atttcagtat ttagaaatgg
     1381 atttgtgccc acgtgcatac atgatgtgct attacaagat ctgccaaact cttttatctt
     1441 ttctacttct gggtttgact gattctgtca gtgtgtgcct gtactgagag cctgttgggg
     1501 ttgcagatcc cccaggcctc ctgcacatgg cagtctgtcc tagaaacatg ctgctatagt
     1561 cagtctaaag gagcgaggct gctcacggag ctctgacagc t
//
LOCUS       KCVB01000288            2115 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13339pri ultra-conserved element locus
            uce-275, sequence.
ACCESSION   KCVB01000288
VERSION     KCVB01000288.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13339pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2115
                     /note="ultra-conserved element locus uce-275"
BASE COUNT          622 a          440 c          460 g          593 t
ORIGIN      
        1 gcgaccatga ataaaacaac cgctctaggg gaacaatttg ggacgtctga ttttctctgt
       61 gggtgttgag atgaataaaa aaggcccttc ttgagcatgc atgtgtggtt gtctttggga
      121 gagcggccat gccttgtgga cagataaagg agtgatagga caccggggga tagggcaagg
      181 acacgtggcg gcccgcccct ggtcattggt gacaagagca gggggacgtt aaacacacac
      241 actgggctga ctccacactt tgatttagcg cttttgccta atgttacaaa ggggggttac
      301 agaggcccac tggagatggg atcagcagat ctccctgact gtcgtcataa agaagcctgc
      361 ggagggctca aacctcgact cacatctgat gcacgagcca aagctagcgt gcatgtgtgt
      421 gtgagcgaga ggaagcgaga gagagggaga gaggggtggt ccatagacca tagagaacaa
      481 tacacaaagg gcatttaatg attccatatt gatgctctgt aaatggctta atttgtctga
      541 agacacacag caaaggcagt actgtgctga aaactgtatt gactcaaaac acacgattta
      601 atacatatta atattagatg aatgactcat taatggatgc atcagacaaa tcataatact
      661 gtgtcataaa taagggcttt taagaattta tggaatatca gattattttc ccagaacagc
      721 actatgatac ggatccaact tccctccata aaagtcttat actgtagatg caggtaaata
      781 atctctcctt tttttccagc tagttactct cactgtgggc tgggagctga tctctgctcc
      841 agacactttc aagtctactt aatgtagcct acacgggctt cacaggctgt ttcatgctct
      901 tatggaaaac aggcctttga aatccctcag cagcccaatc ttaatctcat ttgtgaatat
      961 gagccgagcc acgacaggac caggaacatc ctcgtcttcc taaagcaccg tttcatttgt
     1021 caggagattt ggggcggttg acactagagg agtgatttaa ttgattttaa tcacacacct
     1081 gagataggga gagcgagggg gctgccctta acgattattc agagggccca gcccatgcag
     1141 tatctccgct ctcagccctc tgtttgtttt gatacatcag cattttgatg gataggactt
     1201 agattatgaa cttacaaagc agtcgggaat tctaagcatg accttggacc taatatgcaa
     1261 ataggactaa taaaaccatt gttcgtcaga gaaaagtgta ccagagtccc acacacttgg
     1321 gtgaaaaagc gcttcccaag aagcagcatg caatatggat tttctatttc caaacgttac
     1381 tgtcatcctc ctgacaacgt tgacttatta tctgcagttg actgtctact caatgtttaa
     1441 cggacacaca ctgtgaaaat gttccacagg caatgctcaa ctttgtaccg cttaaagatg
     1501 aatatttccc tttcgcctcc tcaggaagtt ggagccttct ctcttccctc ggcgtaccac
     1561 agtaatttgt tatgaaaatc tagaccaaaa gcagagggga aaagcaggac cacgttgttg
     1621 caagccatta gttgggaaaa ctgtttggtg aacattataa tgcaaaccag cccagggaaa
     1681 gggggaaaaa aaagtggaag acagcctggg gagagagatt taaatatgta ataaactaat
     1741 atttcattta tttccctcac atgggaacct gaccatttag ctgcagcata ctcagatcag
     1801 aacccctctc ctttaatgag tgaagccagc aattatacac atcttggaaa atatttgaga
     1861 atagatctga aatagccatg ctaaatattt caacatacaa gtcactgtta gtattttatt
     1921 ttgtttgttc ttgttatttt gtgcatgtca attaccctga tttctttacg gctttaatca
     1981 caatttaact tcacaattgc agtaaaatta gaaaagtgaa ataaaagtta taaagctgca
     2041 tttttctctg tattttatga tttcttttgc cctcattcct tcattcacag catttctctg
     2101 agctttggtt ttccc
//
LOCUS       KCVB01000289            1046 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13382pri ultra-conserved element locus
            uce-189, sequence.
ACCESSION   KCVB01000289
VERSION     KCVB01000289.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13382pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1046
                     /note="ultra-conserved element locus uce-189"
BASE COUNT          270 a          213 c          271 g          292 t
ORIGIN      
        1 tatatttaat aatggcacaa gagcactgat cacaactttc aaattcaaga gatcacttca
       61 caaaattgga cttggctaac tacaacgata attagtcact gctaaagtag caattaccat
      121 ctgtatttcc ttttgatcag tttgaattgt atcccttaag atttcgtata aataaatgag
      181 tgagtgcatc aattaacttt gtggaactct taataaatat gctaataaat taataattta
      241 aggaggagat gacaaaaaac aagtgttcat gccagtgatt gaatgagtca cagcctattg
      301 ttaactacaa aatgggtatt ggctttagcg tgtggacagc ctgtttctcc ttcttgaagt
      361 gggtgtatct tttcttcatt tgtgcaccga ttggatgatg atattcctca caggtaaatt
      421 tctctttttg gggtgtgggc agaaggggag gtgggtggga actcagaggc tacgctcagg
      481 ggcatctcct ctaatgggca gtcctgactg gcgtcgtgac cactgttgga ataggcactg
      541 cgggagggcg gaaggcgcga ccggtgctcc tcgccaccca gcctggcgct cccatttgct
      601 aggcgtccca ggctccctct ctcctggtaa ggcacgaagg tggagagttt cggggggttc
      661 ctggtcttgc gtacagggga gggatgcccg ggcatccagc aggcgtctga gtggcccaat
      721 tcactgcact cggttgtaca gtttccggtc attgccacat cagggatgga acggatatct
      781 gcgggcagga gagagaacag caccatcaga gtgataaaga agtgataaac atctgaacaa
      841 cattccaaaa aagaggtcgt aaaatgcatt tgtgaacaga aatggtgtca tctgaaacac
      901 cccatgaaac ccacccccct ccttgatgtc tttgcagctg ttgctaagca gtcaacagca
      961 gttgctatgt agctgttgct atgcactgtg taatgagcac tactgtgtat gtgtgtgtgt
     1021 gtgtgtgtgt gtgtgtgtgt gtgtgt
//
LOCUS       KCVB01000290             984 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13424pri ultra-conserved element locus
            uce-558, sequence.
ACCESSION   KCVB01000290
VERSION     KCVB01000290.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13424pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>984
                     /note="ultra-conserved element locus uce-558"
BASE COUNT          260 a          230 c          225 g          269 t
ORIGIN      
        1 aacattttgt gccttttaca cagcactgtg tcactacatg ccctttgtag tccatgtaat
       61 ttgctattgt aactccaggt catttcaaac ccatttcctc gtgtagtgcc attttttttc
      121 aagtgctgta acacagagag aaaagctatg gtttgaaatg agctgtttca ctcttaaata
      181 catgatccac tctcttgcac tcattcaggt tttaatgtct gagaaaagta gcttacatcc
      241 tttcacctag caattactgt acatatggag tcagtgatac ctcattctag cgttatgcat
      301 tctgctctct ttaacagttt cattctttca agcgtcaacc ctcgtacaat cagggtgagc
      361 cacataggga atagcagagc agaggagccg gacctgtcta ttggtgactt tctcttttaa
      421 ttcagcgcac taatcaattg taccaccgtt gcctccaggg gtaattgtat cttaatacta
      481 actggaagtg agtaggaacc cagggaagac agagaggaag aatagggggg agaaatcaac
      541 aaagatagag agaaggggac acgtttcccc agtaaaacct gatggtaatc atcccaatcc
      601 gcttagcctc tgtgactaat ggggtctgtt cttaagggac ctggcacttt caccagggtg
      661 ctatagaagc cattaatgtc ttaacttata caccttctca gggctgtgca gtaacacatg
      721 tacacacaga cacacaccgg cgcacacaca ttttctccct cccgctcact ctcacactct
      781 cacacaaaca gacacacatg cacttacgca gccacactac actcctgttt cattatcctg
      841 gtgccttgtt ggcctctgct ctgctcctgc tagggaggtt aaatgcagga gagctgggcc
      901 agactgcacg gttggaggag aaggggacgg atggctgggg gtggtagtgg tggtggtggt
      961 ttggggagca agagaaaaga tggg
//
LOCUS       KCVB01000291            1446 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13468pri ultra-conserved element locus
            uce-1247, sequence.
ACCESSION   KCVB01000291
VERSION     KCVB01000291.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13468pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1446
                     /note="ultra-conserved element locus uce-1247"
BASE COUNT          353 a          365 c          338 g          390 t
ORIGIN      
        1 cctcagtatc tctagaatat atgcaagtaa atcctcattg gacacattgt ggccatttac
       61 agaaacacaa agaacaagct gctttacttg cttgtcgaaa aatcgaagtt tctattttat
      121 tggaagttct ctaacaagag tttggctccc cggtgacgtt ttcccagtcc tccgcagtca
      181 ccagacctct ctctggctcc agactctccg ctaagaacct gtcgggtaca gacgctgtgg
      241 agtatgtcgg actggacagc tggtgaagca ataggcaccc cagctaggct tcgtgccaca
      301 gccgctctcg ctcctcaggt tgggggtttg gaggaggagg agtaggaaag gagggagaag
      361 agcggctgaa agccaaagtc ctgctgacag aggaccattg tgtggtgcgc cacggctgac
      421 tttggcaccc tgttggtgat caggccagtg tcagagggca cggagcgacg tccaggacaa
      481 gcaggcacga cagcctcgcg ggtcagctgc aggagcgggc tcgtgtgcac aatgagacag
      541 aaacacagag aaaaaaaacg ctccagtgac tctggaggga gagaaacgga ggggagtggt
      601 ggctcaaaga gtgagggagc cggggacggc ccgctacagc cgccagccgg cccctcctct
      661 gggaacacac agagcctaat gagtttgata gtctgctgag ctccataaat catgccatgc
      721 aaaacatttc tcctctctga ataaaagcat ttatcaccct ctgtttattc actcactctc
      781 acaagattga ttggctccca gcagcctgct gagcaatcag ccgtgccgat tcctctattt
      841 ctccccctcg gtcctcttcc ttgagtttat ttatttatat ttttcctctc ctctgtcatt
      901 cagttgggaa acttcggaag gagtggatga gagacaccgt ctccaatgtc ggcatgggga
      961 tgctcaagtc tgtcaaggac tacgtggacc ccaacaacat cttcggcaac aggaacctcc
     1021 tctaatttcc cgctcctgtc catcttgatc tcaccttttt ctaaaagcat ctcgtgcatt
     1081 ttggattaat aatcactaaa catgatttaa aacgagaacg atctttttca tcaaaacgtg
     1141 tcattcagtg ccattcattt attccccccc caaacctttt cttttacatt ataatgcact
     1201 gaaaatgctg taacaatcat tgtcattcac gatgaagctt gtttcttgtc gtggcctcgg
     1261 ctgcggcgac gatgcaacct gacatgatgc aacaaaacct tgatttttat cacatttgct
     1321 tatgtttaac tagttcaaat ctccttgcca acgtgtttaa ctaatctgtt tgtcttcact
     1381 ttccttgcgt gcgtgtgtgt gtgtgtgtgg cacttcttta gtggtaagtt ctagatgaat
     1441 gcgctt
//
LOCUS       KCVB01000292            1432 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13513pri ultra-conserved element locus
            uce-760, sequence.
ACCESSION   KCVB01000292
VERSION     KCVB01000292.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13513pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1432
                     /note="ultra-conserved element locus uce-760"
BASE COUNT          422 a          325 c          291 g          394 t
ORIGIN      
        1 aaaatgcctt ctggggtgtc tcgcttagag acgcagtttt gagcatcaat attccatcgg
       61 ggctgtttaa tgaaaaggat agaaagagga gacaaaattg gaaagagcag ggaactaatt
      121 ggaagttggt tagttaggtg taaattattg gtccttcctc aggcactctg cagaatgtag
      181 tcatactgaa tgagcactag tgaggtacag aactaattat tcggtgtttc ttctggagaa
      241 aatattgagc atttccagct atgtatgcca cattcattac tgaggtactt gtgggttgag
      301 accacaggca agactgggtc ttgacaggat ctggaagcgg aaaagggtgg gaaaatggga
      361 gggaggtagt tataaaattt gtaataaaga aattgagcct attattgttg atccagcccc
      421 acagaaatta gcggaacaca ttctttttgt tcctgtcaac ttgacctccc ccttcatata
      481 aaaactcctc caccccctgc aacactcccc ctacctcctt ttccctcccc aaacccttgc
      541 agcacttcac aatcatactc ccttttgaaa gaactgagga aaaaatcctg ccagagcctc
      601 aattacacaa tggcagaaaa agctccacac actgaccccc agccagctgc attcactctc
      661 tgcacatctc tctccacagg aattctaaag gccttttagt caggcagcca gagtgcagcc
      721 agcgggagag agcgcaggaa gagctgcata cactccgccc gcgccgtccc aagtcttcac
      781 agagagatta atcatctgtg aaatggaaac agcaaacggc cagtgacaca ggctgaaccc
      841 gctccaggtt tccctcatca gctcgcagcc gccgtgcagc gctcctctaa caggcaggtg
      901 ttaataaaag gcatataggt cccacaaggg tcaaacacat atgcctgcca ccatgaagcg
      961 ccgctggtgg gccggtttct tattattgtt ttttaaggat agggattttg aggattcata
     1021 cagacaaaag caatgaaaca ttgttcttgt aaacacagca ccaacaaaaa ggatttaaat
     1081 ctttacaaca atgcatacat atgagcgctg tgctgcaggc gcataaattt agtttagact
     1141 cactatgaaa tatttagtga cctttttcat tgtgacagca atttttaacc ataataaact
     1201 attttggaaa taagtccaaa tttatgattt ggttgtttta tccttgcaaa tcccattttc
     1261 ttaagcagta aaacgtgagc agagctctca tgctgcagca ggacgtgtat ggaaaatcaa
     1321 atcaatttaa accttcactg ttttttcgct tattttgtct ccatacctat cagatattgt
     1381 tttacagtat taatcatcca tttatgtgtt tttgtctcac ccacaatgtc tt
//
LOCUS       KCVB01000293             994 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13567pri ultra-conserved element locus
            uce-461, sequence.
ACCESSION   KCVB01000293
VERSION     KCVB01000293.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13567pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>994
                     /note="ultra-conserved element locus uce-461"
BASE COUNT          279 a          188 c          291 g          236 t
ORIGIN      
        1 ctatctatct atctatctat ctacttgtga atttcgacga tgtgtatgtg tttgtccatg
       61 ttcgaagagt ctaggcagta tgagtgtgtg tgtgaaagga tgagtgtgct cctgtgagta
      121 tctgtatgtc tgagctggtc acacacacac acacacacac acacacacac acacacacac
      181 acatgcaggt gaagctcagg ggcccaggac gacaggccgg acgtcgagac gcggggagtc
      241 gtgttggagc ggggggtgga ttttattttt attttgagtg tttaatgtat taaaaggggc
      301 cggggtaatg aattataatc tttaatgcag aaaccgatcg ataccaccaa gagaataaat
      361 cagcttttac tgagagcgtc aaagcccctt aatacactgg gagagagaaa gggagtgcat
      421 gagggaggag gaggaggaag agaaggaggg attgagggga gcagcaggga caaactgtta
      481 ggagaggagg aaggatcctg agcagggaga gagaggagga aggggatgaa gtagaggaga
      541 gaggggagag tgggggtaag gaggaaggga tggagagatg gatggaggcc gagggaggaa
      601 agttatagat gaacagactc acctttgggc taacctcatt gagtaaaagc ccgctgaaag
      661 catgcagatt ttgacagttt tgtgaggagg cgatgttgca gggtcagccg tgtgtgtgcg
      721 cacttgttat tctcccacct aggtgtattg tgttccctct caatgatccc ctccccaact
      781 tctgttgcct gaagcaaaat ataattaatt gaattacact gccacttctc gatcaaagat
      841 cacctcaccg ctctcttgtc tcagtgcctg cccaaccggg ggcctctgct gggagaaatt
      901 gcctaacgac aatccatgaa ttacaacttt ttattaaccc aaagtgaagt ttctgtggtg
      961 tgtgctgatt ctaaatcagc cagactgcag aaac
//
LOCUS       KCVB01000294            1850 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13605pri ultra-conserved element locus
            uce-424, sequence.
ACCESSION   KCVB01000294
VERSION     KCVB01000294.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13605pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1850
                     /note="ultra-conserved element locus uce-424"
BASE COUNT          554 a          384 c          374 g          538 t
ORIGIN      
        1 tcaatcaata acagcgttca tctgacatag ttgctgaatt tcacgtcagc aagagaatat
       61 gagtgctact tgaggcacac caatattcta catgaagcag ctgcaaattg gtcccttgtg
      121 ctttaatcac acaggcagtt caaatcattg actttgcaaa tcattacatt tcacaaggca
      181 ttgttttcaa gactactgat gtattgaaag gatgctacaa agagaagata ctacatggat
      241 ggaagaataa ttacagtccg tttgcagggt tcttacatta atccaaaaca aaagattaaa
      301 ttaaactcaa cggttatagc agcgtaagat gttggaagca gagacgaaca atagagcttg
      361 actcattcta tacagcacaa cattgtctta atttaattta ttgggtgttt atttaatagt
      421 tattatgcag gaaacaacaa taagtgagga ctaattcaat tgcacaaaaa gccatcctta
      481 ggccactttt cttgtgtgtg aacactattt taagctccat ttatggtgtt caaattaaac
      541 atctcggagt tgctggaagc ttctcttgtt aatggaatcc cacatagcgg attgtgtggg
      601 gaggagagca gatgaatctg tattccctga atgtgtctgg gcaaaaatca ataaatgcaa
      661 gcacagctcc tcgcttctga cggctgtccc tgtctttgca tggtggacta atagaaaaat
      721 aataattaaa tccaaatcac cagattaatt tgttttccat cagtctcagg agagatcttt
      781 ttttttcctg tttctttagt ggctcattat actttctaat caagacaaaa aaagtccctc
      841 ttttagacag acatgctgtc tcccttcccc cttctcttcc ctgtggtgtg ctttctcgca
      901 ttgcagtcat tgtgctggca cttattatgt tacaaatgac cgaggtacgg caggcattct
      961 cagtgtgcat gcaaagaggg cccacccttc tatgctgcta gtgccttctc ctaatgacga
     1021 tgctcagagt gccccctgac gccgccccat gcagccctct cccccattcc cgcccccatg
     1081 tgctctcttt aatgaagatt ctccaatgca attaaaaatc cttgcttctt gctgctactg
     1141 tatgacaaac agagccactg tcattcttca tggcctgtca ttcctgcaac aagaggcgga
     1201 ggagatggag aaatacacca gggtgccagt ggctagagag acagatagag gggattgtgt
     1261 gtgtctgtgt gtctgtgtgt gcttggttga agaagagcaa aagcccccat ctcaacaggt
     1321 aatggaaagg ggcagagaaa aaaatcaatg accagagaac atggagcgtg agcttataaa
     1381 atggctttaa ctgcacgctc caagctgaat aaggggttct gtgtccatcc tcaaaaactc
     1441 gggtgatcat gaatgtttta tcacacaccc accaccacca ccaccttctc ctttttttct
     1501 ttctttttat acatgaaaat gactgatttc tgagtaacat ctttgcaact cttgcaataa
     1561 catcaaaaaa cattcgggtg acacttttcg gatgctgtga cataacagag gagagataga
     1621 gagagggaaa caaggacaaa aaaaccagga tgcatttatg gtcatttata atgtttatga
     1681 agcattaata tgaatgtgcc tgtaaaccaa cactccatca agctgctaac aatgagttca
     1741 ttgtttgtgt gtgatttatg agttgctcat aaactaacag taaacagttt gttaatccag
     1801 tataatacct gttaaaatgg atacaaccag gggtgatgtg acatgtagag
//
LOCUS       KCVB01000295            1695 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13693pri ultra-conserved element locus
            uce-1082, sequence.
ACCESSION   KCVB01000295
VERSION     KCVB01000295.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13693pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1695
                     /note="ultra-conserved element locus uce-1082"
BASE COUNT          425 a          399 c          426 g          445 t
ORIGIN      
        1 ctttgaaaag taggtctggt tcccagccta tggctatagg tgcaggtttg tcttatgctg
       61 caattactgt ccacgtcaag atggctgaca aactgggagc gcccatcact tctgaaaagt
      121 tgcgctggct gagagccagt atttgttggc atggaaagct gttcttcatg cgttggccaa
      181 tctttgacag aacaatcgct ggtacaagtt ctctatgtgc tacggacagc tagtccggat
      241 tcactctaaa tattaccaca ggtcagatga aaggcagtgt gtggtattta aataggccgt
      301 gcatcaaagc gtagcaattt gagagtgaca tgtgagtctg gaaagtgcta atagccttcc
      361 caagctgatt ccatccgacc ccctgccaat gtcagaatcc agctgaattt gctctccaag
      421 atgttcttct gaggtgaaag ccagcagtca gttgtagctc cactggtagc cgtttccgat
      481 gctttggtag cgtagcatca aagtcacggg gcacacatat attccgcagg attagagtag
      541 cgtaaaagga cagccccagc ccattcactg ttggcacggc cgccactgcc tcggcacaag
      601 tgagttttta atgcattcca tttaacttta attactccaa tctctgcgag ggcacacagg
      661 aagaaaaggc ccgggcgttt gtgccgagca gattacccca ctgccctgcc aagtccgcgg
      721 cgcgctgcat cctaagatag aattcaaatt caaatgtggg ctaaagaaag tgcagctgtc
      781 acccctctgg cctacgaagc cccaaccgga tcaaagatga gctgtcctcc tcccctgccc
      841 gtgtaaaaat gagctgtcct ggccgtgcca gtgtctcgtc cccctggggc acctaatcct
      901 cctctcctga ctccacactg acagtctgaa taaaggcatt aaaccccgca aaataattac
      961 caattaaaac ggatggagaa gtcatcaggt agtgactggg gggagagcag gcagaggcgg
     1021 cagagaggga tgaagcttcg gtgggtttct ctcttccctg ccaagaacaa cctgtatttt
     1081 ctcctttatg ggttatggtt ttgcagttct ggatgagtga gtgaccttac agagtggtat
     1141 cagaccagga acagacttct gaaagcttct aggatgtcga agaaagtttg tgtgaggcat
     1201 cccaactgtg cggaagccag aaatcagtac agaagtttta taagccactg gaattaagag
     1261 tacttgtttt atctggtgct attccacctg ggatgtggct gcatagacct gagatgttgt
     1321 gaatctatag aggtcactgg gcattttgtt gtgtgtaacg tatagatgtg tgtttggcat
     1381 gtttgcaggt acatgagaat gcatttaaaa gcattggtgt gttcggacaa aagaccaaag
     1441 cacatgtgtg cttaagcata atgaaccgca aattcatgtg tttgtgtgtt tactggccac
     1501 tgtgctgtaa atgagcatgt gtgtgcatgt gtgatcatca aactgtacaa tagtgttatg
     1561 ggggtgtttg cagactagcg tgccatgagg cccggggcac taatccacca gcgccacact
     1621 gccgaatcca ggtaaagtga tcctaaagcc ttcttacccc tctcccgcct ctgttctccc
     1681 tgctcccctt tgagc
//
LOCUS       KCVB01000296            1782 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13736pri ultra-conserved element locus
            uce-1263, sequence.
ACCESSION   KCVB01000296
VERSION     KCVB01000296.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13736pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1782
                     /note="ultra-conserved element locus uce-1263"
BASE COUNT          525 a          348 c          343 g          566 t
ORIGIN      
        1 gtgcattctg ggaagtggca cataaaagaa tgatcaggca aagctttcag acagaagtcg
       61 ttcgcacagg caggctcttt gttgaacaaa atggaaatgc agcaacgaga ggtgcacctg
      121 ttgagtgtcc ttgtggagac acaagcactc acacttggca ttatgtgcct tattatttat
      181 atggggctgt cagctaataa tgcatgggtc tctcactgga gcactttctg ccatctttga
      241 aagtcatggc aagaggcacc catggttcct gagacatcag gggtcctctc accagggtgt
      301 tgtctgaaaa agtcattccc acttatactg atataatgca tcgtgctgcg gtgagtatag
      361 agcagccttc cttatctctg ctcacttatt tacttcttct ctgtcttctc ctttattctg
      421 cttagtgcat cagcctatat gttcctgctc agttgataat tttaggattt gggaaatgat
      481 atgagtaaaa cccatttttt ttactttacc ttgaaagaaa tcagtataag ctcacgactt
      541 tagatcgggc actctcttat aagcactggt tagtaaccac atcaagtatg actcatccta
      601 tcctaagtca catctatgta ccgctgtttc cgtacgcttc actgtaagca ctgagattgg
      661 tctttaaggt ttgagggcat tgttcactta tcatgtgata ggaacatttt ttatgcaaga
      721 gcgagccctg attagagggt gagttttaat gacaactaat tagccaacct ctttagcctt
      781 ggcgtaatga tcaaacgagc tgtcatcggg tacctgatac aatgatcctc tcaatcttga
      841 tatgatattt tattcctctg caattatgca gcccccactg caccacatgt gtttattgcc
      901 tcaagcacac atgtcaaatt aaagagcagc ttatgcacct gatattcaat tattgcctgc
      961 tgcaccacca tgcacaatgg gaaaatgtca aagccagcaa tctacagtac tttaagcagc
     1021 aatcggtacg ctgcgatcac cgagcactca tgcatataca catacacaca gataggaatt
     1081 tgaatattga atatgaatgg gaacattttc cagcttgtct atgctcagtt gactttttat
     1141 acttcccttt gttactgtaa agggaatttg gcggatggca gtgttgcttg ccagcaggaa
     1201 aagcttaaag cgaatgtttc cttacaatct gaaaagataa atcacaacaa aacatcttaa
     1261 aatatattgt agaaatagat aattattata tgtgatttaa ctaagtaaat ccatttattt
     1321 cacacagctg ctacattttc actgactttt atttcatttt cagagtattt ttccaaaagc
     1381 atttgtggca cgttcataaa gcaacaagaa tatgattttc catttcaggg aaattagacg
     1441 gtgtagacat ttttgaagga ctctgcttaa aatgatccaa catccagggg ttcttgatag
     1501 tatgagtata tttgtactaa agtagctaaa atgtgcagaa gcacttaatt tatgtatata
     1561 agctgaattg agagcagaga cacatgaaca catattgagg agaatgaaaa tgtctgagag
     1621 tgacttcacg tgtttataat gaatactgtt gtgtggtaaa aatccagcta taaagactgt
     1681 atttctatgg ataattcaat tatttcacaa accatgaaag tttaattatt cttgaactag
     1741 tctgtgagat catttctagg tctttgtctt ctctgcagtc cc
//
LOCUS       KCVB01000297            1142 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13778pri ultra-conserved element locus
            uce-214, sequence.
ACCESSION   KCVB01000297
VERSION     KCVB01000297.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13778pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1142
                     /note="ultra-conserved element locus uce-214"
BASE COUNT          300 a          247 c          265 g          330 t
ORIGIN      
        1 gtaatttgct cgatacacac taattgctct aatttgtctg tcttgtttct gtttgccttt
       61 gtgtgtgtgt atgtgtgtgt gcgcacatgt gtgtatgatg tgtgcttttt ttatgtgcac
      121 agtctctgga tggctttgta ttcgtggtca gtcatgaggg acggttcctc tacatctcag
      181 agacagtttc tatctacctg ggactctcac aggtaagacg accttatagc atgcactcac
      241 acacacacac acacacttac acacacacac aaactggaat aatcctttgt atacaagtgc
      301 atttgcacac atgtgcctat cttctgctta cgctttcata tcccctccca ggagtgtctg
      361 tgtgtgtgtt tgtgagtgtg aatatctttg actgcactct ttgcgctcat ctctggagtg
      421 caactctcac gggggcaatg ctactctctc tctacctctc tctaccgccc tctctccctc
      481 tccaggtgct cacctctgtt gattgcctgt ggcctcttgt actgtgaatg ttaagtcctg
      541 ccattttgcc cagatgcata aataataaca gtaattactg gcccagcaat caaagtgtcg
      601 ccccagcgcg ctgggcctgt ctaggggaac aaatcgatag gcatcttaaa agccacattg
      661 attgtaggga gggggaaaaa caccaagggg gatgggaaga tagatagaga cagccggaga
      721 gagaaagaga gggggaggaa aggaggagag gtgtgataag agagggatgt ttcgaagtaa
      781 gaagactcag tacagcatga gagagccaga actgagtggg tttttttaaa gtggaaattt
      841 gctgtgattg aggtctaccc ttgtgacatt aatggataag tttggatttt ttctactctg
      901 tcttagtaca atactcacat gctttatgtg cattgaaaga gtcattggcc actgtaaata
      961 ctggctgtga agcggcatcc tcctaatacg acttcactat aagggggaca aaatccacag
     1021 tcctcgttct gtgtaaaaat gcactgtaaa gcacagcaga caagacaaga cacaatcaat
     1081 cacccgccaa agtcccagtc attttagtat aaaatgtctg caatgtatga atataatatg
     1141 gc
//
LOCUS       KCVB01000298            1520 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13822pri ultra-conserved element locus
            uce-251, sequence.
ACCESSION   KCVB01000298
VERSION     KCVB01000298.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13822pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1520
                     /note="ultra-conserved element locus uce-251"
BASE COUNT          479 a          270 c          256 g          515 t
ORIGIN      
        1 ataatcacct cattaggacc acactgccaa aagtaatgca actttatatt taaaacagga
       61 gttcaaaaat gatttgcatg agctaattaa gtaagttaaa taaacatttt acagtgtgtc
      121 agatttactt aaaagagctg atgcaaattc ttaactaaat gcaatatcgc tagcatcgct
      181 tttctcagtg tggtgtcaga acattgtact cattgtctct ttgtcccgtt atgggaaaca
      241 aagagggcat gcattgtact gaatacagct cagaatacat ttgtctaatt ttacaaaggc
      301 tcacaatatt ttggactggg taaaccaaaa aagaaaactc actaagatta caaactttga
      361 ggaaactgtg gatcaatagc atgttatgtc catgaccccc tcctctgtgg ctttccgatg
      421 gcttacacat ttttactttc attttgtttt cttttctttt ctgggtgtgc aggagggtgg
      481 gagggaggga gctggtttga tgacgtgtta gagtcttaaa aaaaacaatg tgagtttatt
      541 tgcatgtttg ctatgtgtct acgctgtgct ttcaagctca attaatactt ccaactcacc
      601 ctcttaaatt cgttgcattg ttctatgccc tagagtaaaa gagataataa atacaaactt
      661 gtgtggtctt tgtttctagc catgtactgc agttgcttca aacatcctga aaaacagtat
      721 gtaataagct gttgttctca gaaaaccatg tttaagccta gctttcatat gaacctctat
      781 tttgtcccat agaatatgga atgatcggag agcatacagt aaaaagtcag cggccaagat
      841 cagttcatga cacaaaggcc ctgcaggagc aagctgaatc tgctaaattt atggcacaaa
      901 ctggtaatac aatagttatg tttctttact ttattttgtt aactaaactt aatttcatca
      961 tgcatgctct ctcttgtact gcacctttta agtggaaatg ttttgcatta ttattttttc
     1021 attctctatt gctacatatg aactaggaag ataaatttcc catttctcta tgagtattta
     1081 ctttgaattt agaacataat tgggttttgt gggtctgttt gttttattgt cacatagtta
     1141 gttggcaagt ggattactca tacagtatat tcatagaaca ttaattaaac agcagtttaa
     1201 aactaaggcc ccaatgtagt taatcatttc ctccaaggca tgataaatta aatgtcattt
     1261 gatgaaaaac ccaccccttc agccaaatat gaaacacata cttggcaaaa gttaaactga
     1321 tagatgctgc cctcagagat aaaatgaagc tatgcggctt aatcaagtgt agcatccaat
     1381 aaattaaaaa ctgaaattac ctacattcat aatgactttt acaacatacc accctagcac
     1441 tttaatgaaa tcagaaagta ctgaaataag caaaacctta attttgcttt tatttttttg
     1501 ttcttttttt gctttattct
//
LOCUS       KCVB01000299            1293 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13865pri ultra-conserved element locus
            uce-638, sequence.
ACCESSION   KCVB01000299
VERSION     KCVB01000299.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13865pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1293
                     /note="ultra-conserved element locus uce-638"
BASE COUNT          390 a          239 c          315 g          349 t
ORIGIN      
        1 acaatgttct tgaactgtga gttgtgaaca gctgcacagg tagcattcag atccttcact
       61 taagcagaaa gagcaacatg aggtcgaggt catttcaact gcagtctgga agtttaatct
      121 acagaaatgc atcaacaaat ttaagctcat catatgtttt gtctttaaaa tcctgtaaag
      181 taccagtaac tagtaactaa agcagtgaga caaatgtagg agaattaaaa atagtatttc
      241 ccactagtag agtggaaata tgaagttgta ttaattgaaa taaataaaaa gtacctcaaa
      301 tttgtactta agcaaaatac ttataaaact ttttgcccat atatctttac tttatgtttg
      361 ataacaactt taaagaaaag aaaactggag gtgaggttgc agcgtctgta agacgtgcat
      421 acattgttaa agcaaaagac aacagtgaag aagtggagga atgagagttg gtgggggggt
      481 cggaaaacat cttgatgggt tgtgtccggg aggaaggttg aggtgggggg ttgggtggag
      541 aggcttgatc tcggggtgcc ttgggggggt cctgagtatg atttgatcct ggaaggctca
      601 attaagtggt tcgtttaccc ctcgtgtgga gccggtgaat tggagccagg tcagctccgg
      661 gttgaggtgt tgttcattat ttttccgcct gtcggatccc atgagcggcc gctggagaca
      721 gtggctgctc ggcggccgag gagcaaaggg tctgccggtg gggtacgtca aacacactca
      781 aagcctctcc cctttgcctg ggggattatg atcaacaaag catgtcccac acctgcaccg
      841 gccagcacaa tggaaactct gcgggccaat ggagacgaag ggagagacca gttattagag
      901 tcccccacca atgaaatgga ttacattaac cccttctgtt gttgttcctc ccagagctta
      961 aacatcgaac tgacagactc cacatcccat tgagaaaaaa tcccagtaac actttgttac
     1021 aacagcttta ttccggttta tccaccaatg gggaagtatt gtgtctcctc tgctaaactg
     1081 tgtgatgact tggtgaagtg ttgtaaagct gtgctgaagc tttggctgac ggatttaact
     1141 aacagaactt tctgttgtta ctgtacaaat taatggagaa agtcattaag aaaatgagaa
     1201 agtaagcagt aatggacatc tttagttgtt ttttacaagt aataaatgga aaataaggaa
     1261 ataaaacaac cttttctcac tcaaaacccc tca
//
LOCUS       KCVB01000300            1858 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13905pri ultra-conserved element locus
            uce-115, sequence.
ACCESSION   KCVB01000300
VERSION     KCVB01000300.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13905pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1858
                     /note="ultra-conserved element locus uce-115"
BASE COUNT          531 a          371 c          467 g          489 t
ORIGIN      
        1 ctatatgttt taagtcttga gttctgtttg catcactgga tcattttgag aatgcaaaca
       61 ggaatgatga tgtaatgata ccagtgagtt tgctggaggc attacaggcc aggttttgta
      121 gcctaaagta gaatatataa tagaaggtct gcactggctt ccagtcgtac ctgtttgttc
      181 tgaataaaag tcgcaactta attattgtaa ggcaagatct gaatttaaaa agtacatatt
      241 ttttacatga tttctgcaga gatgtatctt tatcttgagt tgaaactgga gatatgctgc
      301 tatcactgga aagtcacacc catgagatgt gtatcacatc tgtacattct ggcttgacta
      361 agatatgatt tagagaggaa gggaggcctt tttttttaat gcaaaatgcg ccaagtccta
      421 aaaaggcacc agggaaggct ccttctgatg aaccaatcat taaagcaagc tctgatgaaa
      481 tgccatccgt gcagatgaaa acacagatga agtacgtatg tgagggaatg tgataatgac
      541 aatgggcagg gtgctttgtg atggtgtaaa aaaatgcgtg ccatacttca tcctgccctc
      601 aggatctctc acacacacac acacacatag gaatgcatag ccaaagcttt ctcgctctgg
      661 ggtacttgtc tcataatggc tctgcaaaaa tagcttaaaa attttgttgg ggctgagggg
      721 gaaaaaaaat gatgcatcac aacccattag cacagcatgc cacgagatga tgggtgcacg
      781 tgtgtgtgct tgtgacgact ccacagacgt ggaggcctgt gagtgacaga aaggctaatt
      841 gacaagcgca gactcacagg agcggagagt caatggggcc cgcgagagcg taccttctcc
      901 cgatgatgaa gccgaacacc atgaacacaa ggatgatggt ccccgccacg gccaccactg
      961 cgatgatgat gacggggttc tgctcgctgg agacaatcgc agctgatgag atggacacac
     1021 ataaacacac acggacagac attcagaaag acagaaatgc ttagtgtgaa cttcagcaca
     1081 tcaaagacta caatctgcag gacataattg agcccaatga gaggcgtgga gattgataac
     1141 ccccctcctc tccttccctc tgagagagtg ggggagggac gcttctattt ttgagccgtc
     1201 cttatacaca cggctctact gtttcagaaa gtgggcttaa tagtcccaga cggcgacggc
     1261 aatgcccttc atctcaaaca aagacacatt caggttggat tgtttcccat tcagagctct
     1321 cacagtgctc aaactgtttg cgttgacaaa ctgccgattg ctctgaagaa tggcttgcct
     1381 tagcgtcgta taaagtcaac gtgagtggtg tatgaggggg aaaattcatg cattttaaat
     1441 caagtggatc tgcgctgtat aaatgaaaga gaatgatttt ttggtggcct gtctgaggag
     1501 gaggaggggg gctggaaaat gtagcagggt ttgatcagct gttacttaat caagcgtcaa
     1561 tatctcacca attgtctgtt tttgggagga tttaagagca ctcaactgtc atattacaca
     1621 cttgcaggtt tccctctcga ggctgtcaca gaggcagcta gtggttcact gtaatggtca
     1681 aggacacatt gactggactg caaactttta accttgttac aggacgcact ctgcatgtgg
     1741 tatgagtaat taatgtgtcc ttttatgcag aaatgctgga tctgaggtag gtgggcgcat
     1801 gtttggctcg gaaggtcgga tatgggggtt ttcacaactc agaaaagggc tttaaaca
//
LOCUS       KCVB01000301            1835 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13941pri ultra-conserved element locus
            uce-956, sequence.
ACCESSION   KCVB01000301
VERSION     KCVB01000301.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13941pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1835
                     /note="ultra-conserved element locus uce-956"
BASE COUNT          503 a          407 c          438 g          487 t
ORIGIN      
        1 gacacattaa taggctgaaa aaactgtaac atttctgggt ttggtgggtt atcacagccg
       61 ccctgtttgt tttactctct gtcttgtttc agtttacgga catgacctta atgtggagat
      121 gccgagtgta caagcactcg catccaggga cagatcaagc atagaaatga ttaacaaaac
      181 agcggggttg gatattacag tttggctggg ctccagtggt ggaggtagtt tgggttaaaa
      241 agcaacaaag gcacatggcc ccgacagacg aacacaggca ataaaattta cgagggtcct
      301 taattaccga ccctgctgct cgatagtcgt aaattgctgt tgtaagggct gctactagtg
      361 ctgcctcaat aggcttgcgt gtgttccagt ggttgtttct cagtggttct gctatttaac
      421 cgtgacgata actcaccggt gctaacgtgg acctttttca tccaaggata gaccaccggc
      481 tccttgccct tcgctacgcc gggataaacc tcaccggcga ggctgcagtc tttgctggca
      541 tttgcccctg cgccaccgtc tggggccgcg gtgaggcgag gaccgtggct ctgtggaacc
      601 ggttgcggct gtgcgtggtg tccatcgccg aaatcgtcga gtccggtggc gggaacgtta
      661 ccataatcgt agcctgagtc tgtgtagttt gacctcggat aggaaggctc gtcgtgatgg
      721 gggaagcccg tatcttttgg ccgctcgtag taatcaccag ggttggggat gtatccgctc
      781 tgctgatatt cgtcgcatgg aggaaaggaa ggctcgatat agttggagtt tatcaagtag
      841 gaactcatgg tcattaattt gtgaagtgca acaatactaa tttttatcgc gctgtcgttt
      901 ttctgatctc cacaaaaccc tcctactccc tgtcaaatgt acaaagttgt ctggtcacgt
      961 gtgaagcgcc accaatcacc gcgtgtcctg tgggcatcat gtaaacagga accaatggaa
     1021 agcatggcac tggtaccacc aacactgctc gcgctggctt acgttggtgt tttccttatt
     1081 aaatcacagt atataaagca aaaccaatca actaacttgt ttactgcacg gggccaaagt
     1141 tatgtgggtg aggctttcct gcatgtgaaa aaaaaaattc catctcctct ggaacagaaa
     1201 gtggatatga aattcaagcc ctgagaaaaa aaaggatacc agagacaaaa gagtatttcc
     1261 aatagagacg tgaatgtctg ctgttttgag tttacaatgt gtggaatctt cataaagaga
     1321 agagcaggga tacaaaaaaa gacaccaaac aacattgcaa acctaatgtt gtgtgtaagc
     1381 gtcttaaaat aatcgaaaaa ggaaacagtg taagtgcacc agcttcatgc aagcactcac
     1441 gagccgctgc tctgcggaca tacaagcctg catataacgc atttaacttg tgtatcagta
     1501 aaacctggtt tatacccgct tatgacacac acataaagaa gagaagcgcc catttctcac
     1561 gttgcacagc tgtgtaaatg cattttttct gcagtattcc caggacatag caacaatttc
     1621 tagctatttt tgctctctgt ttgtcctgta atacaacatg gaccttatct ggcactgatc
     1681 gcacgtatta tcagtgctgt ttaactgtct taaccacaca tacacttgca ggacacgcac
     1741 cttgcattac agccaaaaca aagcgtggga tatgtaacca ggatggaaca tttagagtaa
     1801 gagatgccag aatttctttg caatgcatag ataat
//
LOCUS       KCVB01000302            1471 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 13975pri ultra-conserved element locus
            uce-581, sequence.
ACCESSION   KCVB01000302
VERSION     KCVB01000302.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="13975pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1471
                     /note="ultra-conserved element locus uce-581"
BASE COUNT          428 a          303 c          356 g          384 t
ORIGIN      
        1 cagggcaaac agacttgtaa atattgagac aagcgctaag gcatgaggaa aagttgacac
       61 tttattttat ggagctggta caattgataa attgccactt ctctgctgat aaaaaagcat
      121 ttcatgtagc attatgaatg catcccctga aatctgcgct agtttaatct aatgtactaa
      181 aaatggttga tgtcctgtgg ataaggtcct aaacagtggg agattacact gctacaaggg
      241 cagagcatgt ttagcagaca gccactttag ggggtaatgg atgtgtgctg catcgcattg
      301 tacctttgta agtataccat cctctctgtg gttcttctgc aggacgtgct ggagggccag
      361 ttggatcttc tgctggagtt tctccacctt caccttctcc tggagccaag atcggtctga
      421 aaggtaaatt atcacaacat atttattggt atttaaatac attgctggct cagtagcagc
      481 caacttggct cattaaaaaa gtaataatgc agtgccacta atataagata ttgattaata
      541 gagaaaagct gaaggggcag cagcgtttct tgttgtggtg aatgtgtgtc tatctttttt
      601 ttcgctgcac tgtgagtgac acctaccagc agacatcaac acaaaggcgg agaacagggc
      661 gatctcatcc tcagacaggt gcatagaaca caagtttttc ccaaactcga agacggagct
      721 gatcaagtcg tcacagcctg ccacagcaaa ggacacagag agagggctta ggatgaggaa
      781 gaccaaagaa caacattcac ttttgatctt tattgggaga gaggcacaca gggaggggag
      841 aggaagggga aggaatgaaa aagagtggca tgctgacagc acatgggagc gtcttaagca
      901 gaacaggcaa tgcttttact gagttttgct tcccagacga gtcacttttg gcagttgggt
      961 ggtgttatgc aagccaaaat gaggtcacta ttgaggcttt tagctaaatc aattatcgag
     1021 gatagaaaga gacaggcgga tgccaccgcg gctcgggaag cccatcactc tgcctccccc
     1081 actgcagacc ccgctgcata gaacacacag gacactggga ctctcgaagc tgggacagat
     1141 acacatgctc attcatactc acatgcaaac agggagttac aaggaatggt aagcttgcac
     1201 cacaaaacag aatccagcat ttttactgcc actatgggac gcatttacat ttttttttgt
     1261 gtgtgtgtgt caaaggcagt cggaaatgcg agaaaatcca ttgtggagag tcttactaat
     1321 gtcatagcag tcatacacga atgctatttg cttttgcctg agatgtttta tgtcagacta
     1381 tcaatgatgg ttttgatagc gctctcccta aagacactcc gtctggcttt atttattcca
     1441 cagttacagg agccatatgc agcagagcct c
//
LOCUS       KCVB01000303            1360 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14013pri ultra-conserved element locus
            uce-150, sequence.
ACCESSION   KCVB01000303
VERSION     KCVB01000303.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14013pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1360
                     /note="ultra-conserved element locus uce-150"
BASE COUNT          443 a          265 c          327 g          325 t
ORIGIN      
        1 gctctctgtc ccgaagaggg tgagagaaag actcattcca ccgcggattc aggtttttat
       61 aaaccacttt gcttttatag agctgtttcc cttcaagttt gaacttcaca taaggatcac
      121 tggtacctaa aattaaataa aataaaaaaa ttaatacaaa aaagaacaaa tcatcaagac
      181 aatgttgtgt gccacatgca gcaattaccc caagtgttaa gttaatatac aatcggagta
      241 aaaacactga tttcaaagca aactgcggtc aaaatgtaat gcacactatt cctacttcgt
      301 gtggagcata ttgtcacttt aaaaatgtac actatgtatg aaaacacaca catacacacc
      361 cttcccccat gctggttgtg tgagagactg aaatatgtga ggaggaggtg caccccacac
      421 atggcaaaaa cagagggatg tagctgttta attgtgaaag agtagaaaca ctcgccagca
      481 tgaggcaacc agaaaccaaa atctagaagc aaccagtgtt aatctcccaa gcctgttaag
      541 aaagtctgtc tgcgagtggg gtgggggtac gggacttctt tgtttctgca attgcatgca
      601 gattggccaa aaaatgaaag ccagtcccat gccttcattt gtatagggga ataaaggggt
      661 gcttgagtga gcctgcaggg aggcttgaat cacatgacaa aggccctgtg gggatgggct
      721 gtcaggcctg tcagctcccc actccaaatg aacatctcgc aaaggcaggc aggacccaca
      781 gcagctccac cctgccagcc tgtgacaggg aaacctccag gcaacttgac aggaggagaa
      841 tgtggcagct agcaaggaga aaagctctgg catgactgag aacggtgacg atggaaatta
      901 atgggaagaa aggggagaac aaaaactaag gagtgggggc tggtggcgtg ggtcctttca
      961 gaatatgtaa tgacaatgta tgccattggt gagcctttgg cacggtgttt attgttgggc
     1021 aaacagtgga gagacagggg gcgtgtgggg attgcatcat catggtgcca tcttgtcagg
     1081 gaaagtaagg tctcaactag cagggagtag ggcacggagc ttggagagtg tcctgccacc
     1141 aggcaggaaa aaaaaattgt taagcatcat tccaagaaaa attttccttt caccattttg
     1201 atgtaaagtg atttcccaaa gttacaggcg ctacagcaaa gtataataca gattaaaatc
     1261 acagcatagc ctaagggttt aaattttaaa ctaaataaga ttgatgtagt ttaaaaaaaa
     1321 tgttatatca caaatttcta caattacatg catctattac
//
LOCUS       KCVB01000304             964 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14095pri ultra-conserved element locus
            uce-605, sequence.
ACCESSION   KCVB01000304
VERSION     KCVB01000304.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 964)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14095pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>964
                     /note="ultra-conserved element locus uce-605"
BASE COUNT          306 a          166 c          234 g          258 t
ORIGIN      
        1 cacttggaga aggaagggaa gggtgggggc atcatgagga agaaactttg tgacacttag
       61 gaaccaggat ggtgttcagg caactgttta atttgaggaa aaaaaaatcc tgctttataa
      121 ttattactgc agttgtgaat ataaagtgtg ctgcgctgac tatggaggtt ggaggagtta
      181 agtattatta ttggactagt aaatccatga ccagtactgt tccaacacca atagaggaca
      241 agccattcca tacagatggc ctcaaggaca attagcagta atgattggtt gccacataaa
      301 tgagggaaat tctatttgct actgcacttg ctgatgatca ccgacaaaat gcctgtgaca
      361 ataatataga tggctgcttt atacttgaat gttacttgtg aacgctgctt aaatatacaa
      421 tgaacaggaa atgaacattt cttaagtatc tgtgttattt tgaagggaaa acaaaataag
      481 gaaaggtaga gctttaaatc ttatacaaac accacattag catgaagaat aaagagatga
      541 gacaaacagc atagtgaact aaaatattat ctcttcaatc atatcctttt ccccagatct
      601 cttcatttcc ccctcccttc tctgtcagtt agtctggcaa acaatcccct tgttagcaaa
      661 ctgttaagac agccgcttgg tacacaaatt gcgctcgacc aagcgaattt gttttgcaag
      721 ggtgacctgt ttaatcatct cattttcttt ggcggacatt tattcatctg gtttccacgg
      781 ttacagagaa cattaagggg ctacagtaat aaggctggag agtcaaacag gtaataaaaa
      841 aattaacgcc attttccagc agccgaggac agtgggtgaa atgaatcaag ggagagggga
      901 gagaagcagc ggggggagca agggggtggg gtgtgtggag tggggggggg gggctccaat
      961 attg
//
LOCUS       KCVB01000305            1158 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14139pri ultra-conserved element locus
            uce-291, sequence.
ACCESSION   KCVB01000305
VERSION     KCVB01000305.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14139pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1158
                     /note="ultra-conserved element locus uce-291"
BASE COUNT          354 a          216 c          229 g          359 t
ORIGIN      
        1 tgcatgtata tatatataca tgcatacact catgtttttt tgctgtgtat gcacacattt
       61 tttccccctt ttttctcttc tatcagggtg gtttgtattc atgttttgtg acatcttttc
      121 agattgatat ttttcataaa aagcactgaa attctacaga acaatttata caagtcaaag
      181 gaatcatttc agatgaaagt tgaaatgcta ttgtaaaatg gcctttcact cctcgcgctt
      241 gaagataaac aatttcccat gaatatatgg ctgggccccc tatctatcac aaaaatcaga
      301 atgtgtccca tactgagccc attgtagtct attaaaagaa atccttccct gtggcaacca
      361 ttttgcaggt ttcaagtaga taaatgcctt ccaagaggga taagaatgca tttctacaaa
      421 actgcattaa agagtaatgt ttgtcaacta ctccgataaa aacttcacag tcacaattct
      481 ttttagaggc ggcaaatgca ttggcaatgt gtaaagctga ttggtctggg gaggctgcct
      541 acaggttagc aggcctcatt tccaatctct gacagcaata tgtgtcgccg cgcgcttcat
      601 cccgattgct catgttcaac acaaaccatg gtcactctat atatggaaat ctctgtcttc
      661 agggcgaaaa aaaattaaaa aggtgagatt ggcactttaa cctccatgcc cccctgaaaa
      721 tatgttttag tctgatacat tatactactg cgaattattt atcaagaaga tacaagcttg
      781 atatttcaaa aaaataatga gatgttgagg tggcagctgg aatgttctgg tccagcgagc
      841 agcatctatg aatcggggga aatgaagtaa atatggctgg ctgggacaca gcatgtgcag
      901 aatgtgaaag agtggagtgc agtgtttagg tggccctgac caaaaacttc aaacctgtcc
      961 tttgtggctg tcttttaggc tcttttggaa tagtaacacc aagtttatac gtgcaatgca
     1021 gcattagtta ttacaacaat tagatattta tgatttcaag tttcaaaaag cagcgtttgc
     1081 ttaaatgttt aaagtgcttt gagtgcagcc gtatgctgca gataaaaatc tgacgtgttt
     1141 ggaaaaagaa gtcaaata
//
LOCUS       KCVB01000306            1065 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14180pri ultra-conserved element locus
            uce-640, sequence.
ACCESSION   KCVB01000306
VERSION     KCVB01000306.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14180pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1065
                     /note="ultra-conserved element locus uce-640"
BASE COUNT          293 a          266 c          184 g          322 t
ORIGIN      
        1 ctctctctct ctctctctct ctctctctct ctctctctct ctctctccct ctctctctct
       61 ttctctctcc ccctctctct ctctctgcct cctctgtctg ctcctcacgg ctgggcacag
      121 ttggggagca gctccttatt cattcatgag agaagcaccg gataaataaa aaggttcatt
      181 actgtgctaa agtgttacta atgacatgta cctgccactg agcacctccg aaaatctaat
      241 gtgacacact agaatggaaa atctattctg ccgcaccaag gcctccaatg gtgctagcac
      301 atactctatg gaaatttctc tccgcttgcc ctccaattgt gtctctctaa aaccaatatt
      361 tacaatccca tagctattat gtcctactac gccctctgcg cctgctgttg cgacctccat
      421 attagctgaa aattggactt tgcaaactta gcaggggaga agagactctt ctcatagatg
      481 agagggggca gtaccagggg actcagcgtg aggcttgcgg tgcctctcaa ctctctgctg
      541 ccccaacccc aaactgacaa cacccaccaa aaccaaacag aagtacagaa atactcacta
      601 tgctgcattg catcgttcag tctcttcagt gcagtcaaat tcaaagtgtg taacgataaa
      661 atgccaatga aacatttact gaaagacacc ttatgtctga gtttttattg tgagaacaat
      721 aaaaaaagac tttgtgttgt tttccatgag gtctgagttc tcttttacag cttaaatgag
      781 ccacttgatc acgtacattc agccactggc ttataggtta tgcaaataag cacttaagca
      841 gtaagaatgc ttttgtattt gtctggttat tagccaaatg cagcctcaaa gtttacttcg
      901 caggagagga cctggatgat aatttatcac ccacagtaac aacaattcat gcctttaaag
      961 taatacttgt agatgtttta ccacttaaat cagatgaata tattatgtgg ttctcaataa
     1021 taataaaata taatgagagt gttattattc tgtcgcaact gtaat
//
LOCUS       KCVB01000307            1236 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14224pri ultra-conserved element locus
            uce-128, sequence.
ACCESSION   KCVB01000307
VERSION     KCVB01000307.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14224pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1236
                     /note="ultra-conserved element locus uce-128"
BASE COUNT          349 a          246 c          256 g          385 t
ORIGIN      
        1 attattgaag ctgcgtagtg tcaattcatg gaaatatgga taggatgtag tttttataat
       61 ggcatggcat tagattgcta cacatgagtg gttgtaattg ggaagacacg gtagatttaa
      121 agctattttc ttgatagtgt gaggctgaga agtgtcattt tgcacttaca gcttttcttc
      181 atacccgcaa attgagcata attttgtcat tacgttgtca acaatacaaa tgttcctcgc
      241 acacttgttt attttcactc atggtgtctt agtttgtgtt ttgggctgta cagattattc
      301 tgtgtgttgt gttacgttta tgatttatag gtatttcaga cctgctgcta tttgtttagc
      361 tgtggtcact gaggatatag gtgtcactag gactgactta ggaaaatgca gagcagccaa
      421 aacataaagt ggaaatgtta acccaataac gttagataaa cagtcagcgg agaggagcca
      481 aagacaacag agagcgaagg gaaagtgtcc tgctggcatc aggtttccca gagtaagtgt
      541 tatttgaaaa gcagctgaag ggtgacactc actctcctcc cacccaatct ccctctctcc
      601 ctccctcctc cctcacttac tcgaccatct gttgttccct ctcttctcag gctgctctaa
      661 gtgccttgaa acagctgtcg gagcagggac tggacccggt ggatggcccc atcaaggtac
      721 ggtaggacac ggatcacacc aaactcccag acagatgctc catgctcgct cagtgttgga
      781 tgtttaacca atcagccagt cagagttggc agatgctgga ctagtgacga gccagatgtg
      841 cgagtgccag tctggcttat tgctaatcct ctgcgctgca ggctggcaca tcatcagctc
      901 cctccccacc caacatggtt gggcagtaac tcatgacaag taatgtagat gagtaaaaaa
      961 gtattttttt ttaagaaaca agaacttttt tatggtcttt ttttaaagct gtacttttgc
     1021 tcttacacaa gtatattttt taaacagtaa tctaaacact aagatctctt attctcctct
     1081 cacctctgaa gcacagtaaa gtaggctgca cattcaatta atcgatcttt gaggttatga
     1141 tattgacatt aatataacta gttgctgtag tgatctttta gtttttaaaa taaaataaaa
     1201 attaaattat aaatatcata aattaatatt aaaaag
//
LOCUS       KCVB01000308            1382 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14265pri ultra-conserved element locus
            uce-504, sequence.
ACCESSION   KCVB01000308
VERSION     KCVB01000308.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14265pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1382
                     /note="ultra-conserved element locus uce-504"
BASE COUNT          331 a          327 c          328 g          396 t
ORIGIN      
        1 atacacccac ccacccatcc agccagcctt acactgacat gcatcttagc aggaccctgc
       61 accttttgtc tctctttgtt agcagctggt ggacagagca gcagcctgca gcagcagcag
      121 cagcatcatg tctactttat gtacagtaac agtgtaatga caacacaaac tccaatacat
      181 gagccattat ttctgaatga aggacagaat taattagcgc tcttatgtaa aattgtttac
      241 tgtatggatt ttatttatat acagataaaa aaaacctttg gggggctgaa tgggactgat
      301 tatttttatt aataatattt ataaattatg tatttttatt tatttatgtc ataaaatatg
      361 ggatgtttca actttgtata aacaaatctt tctgtctttc tatctatctt gtctggttga
      421 aagtgtgaag cagcatcatc accaccaaag tgaactgcag ataaccccat gacgtggcac
      481 acacagtatg gaaagcctat ataaaattca cggagcccgc atgtggtggt ggtctgagcc
      541 gctgataagg ctttgcaggc tttctgactt cagtctagat gaaggatgag cttctcagac
      601 atagacgttg tccatcctgc tgatgctccc tcgtctcatc gtaaactctt gtgcaccctc
      661 tggtgcttgc tagtctccca aacttctttg tgtagcctgc tttgcagatt gtcttccttg
      721 tctgtgtggt tgctcattat tcagagacag acggatctca ggtcatctgc agctgcagca
      781 aaggagaagc aggaaagatg gcgatcatcc attagaccga ccgggcgcac gcatgaaata
      841 cgaggacaac ttttgggagg atcgcttgag gagaagattg taccagtgag tttttccccc
      901 ctcttgcact atatttccag tttcgccaag atttcggctg gtctttcaag gtctgcgaga
      961 acgtcgctat ggtagatcat gggatataat ttggagagcg cctcgtgaat gacctggaaa
     1021 gagctgaccc cccctcctcc gttcctcctt ttttagtcca ggtccagccg acttgggaga
     1081 gaaagccggg gcgagatttt ggtcgggtgg gcgctccggc tggcacccat gcagccaaag
     1141 tcaagtgggt cttctcgctt cccagttaat atcctaacca acctcacctc gcttcctcgc
     1201 tatgtcgcct gtcggtttgt tgtcaaagtg acacaaggaa atggacgcgc ggcggtgtat
     1261 aaaagcgggg tcgcctgcgt gtttcagcag caccggagcg gagtgttttg ttattctgac
     1321 atgtctggtc aggggaatta ctggccccgg ctgtgcgtcc cctctccggc cgtgcgtgtt
     1381 gt
//
LOCUS       KCVB01000309            1227 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14301pri ultra-conserved element locus
            uce-996, sequence.
ACCESSION   KCVB01000309
VERSION     KCVB01000309.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14301pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1227
                     /note="ultra-conserved element locus uce-996"
BASE COUNT          380 a          270 c          224 g          353 t
ORIGIN      
        1 gctacatcac atctttcaag ctagtcacca aaacctacta aaacatggac cagttctgac
       61 aactaacttc ctgctttatt aaaaaattaa tcctcatcat ggtttcttac tatattcagt
      121 caaaatcatc cccctattca tgtatagaac cagcatttaa aacatggagc aaatgctctg
      181 cagagcccag ctggctaata tcaaaccata caaatacagt atgtgtttaa ccattcagca
      241 gcaggtttaa aaaagtttct aatcagagca gctgtttgag gctgtggctg aaacagtcca
      301 aaacctagct ggctcttacc tacacaacat ttttaggaca atgaagattc ccaggcaatg
      361 agcatttctg gctggatatg agaagagaaa aactgagtga atgtttctgt ataaagaaac
      421 agactacctg tattaaaaca atctgagaac atgctttttt gccacaatgc ctgatttggt
      481 ctgaatgagg ccaaatgcgc atgaccaact gtacccatcc ccactgaaga caaaagccag
      541 atgttagtgg gtgtgtgttt tttgtttagt gcaaaaaggg tgtgagtgag tgaattactt
      601 gtgtatcctg tctacatagt gtaagcagtt gtccttagca gtacatactg tgtgcataag
      661 aaggtgccca tcagcccgac ttggaccaat gctgagtggg atggaagttg actggctcat
      721 taacagtgga taaacagcct acggccatat gggcaaactg ggaggactgg gaatatccct
      781 tcaatccacc agccacttaa aacactctaa ttggcctctc tgtctgtaga tttaataatg
      841 aaactaggat agccagtctg tgtgcacaag aaacagccat cagttttgtt tatttttttg
      901 gtaactgtta ttttctgtta ttgtgtgttt actgtatgag tcactatatg agcacctaaa
      961 ctaattaaat tcctctgtgc atcggtaata ggaagctaag agatataatt gttgtcattt
     1021 tcatgccatc cttttaatta ctttctggtt ccatttcatt ccatttgaat tgacagcctg
     1081 atcaactttt aattgccagt ttttgcaaac agcttatcag cctgctccaa gcctgcatgc
     1141 agagtactaa atgacctggc caaaacaccc ccgcaaacac attcttaaac acacacacac
     1201 acacacacac acacacacac acacaca
//
LOCUS       KCVB01000310            1496 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14344pri ultra-conserved element locus
            uce-541, sequence.
ACCESSION   KCVB01000310
VERSION     KCVB01000310.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14344pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1496
                     /note="ultra-conserved element locus uce-541"
BASE COUNT          418 a          327 c          287 g          464 t
ORIGIN      
        1 tatatggtca tcagtaaata ataggatgaa gtgcaatgct gtataatcta ttccaatcaa
       61 acattatgaa ataaaaatga tcataagctg ttgccgtcac ctctttcacc atttctttta
      121 taatatgatt tagtcctgac tgtagttatt ctgatggtga gccacacaca gacattagac
      181 aacatctaca ttatttgaga ctttttttaa atatttgaac agccagtgcc caaattccct
      241 tttcaaactt ggcaagataa aaaacagaac tagaaatgca ataatataaa tcattacagt
      301 tcatctgtcc aggtccagac ttcactctct cttgactcat ctgtgccacc agtcattctt
      361 tgtatctttg tctctttttt atcccctctc tctccatatc tctgtgtcta tgctcagccc
      421 attaattgtg cccgaacagt gccacatgcc tcaatgaaaa taaattgctt ttaatttagg
      481 ctttcctttt tgccacggcc ggcatcactt aatttcatta gctctgaacc tgtagagcgc
      541 tttttcaaaa gcatcatctc atggacgcac ttgaaaatga aaagaagccc cgcttttttt
      601 tcccctcctc gctccctctc tcctctctct gcgctcctca attaacagaa tcgtttcctt
      661 tcaactaaaa tgaatgttat tgaaaccaat tagcatggac tggaaaatct ctcgttaatg
      721 aggggagtgt gggtgagtaa acaggaatga aaagatgaaa ttagagaaga ggctcttctt
      781 gttggcctgg aataatgaca tgcttagtcg cctaattgtc tggtgaaaag caccgctgcg
      841 atagtaacag attgcggtgt tcattaggca gcaatgggcc acctgctgct ggggcggcga
      901 gcccgcagtc cccgtacacg tgtttatccc agggggcact ttttttttgg acacaacgct
      961 cgctatgaaa agcaaatgag cttgccaata tgctgcaatg ttgtgtcctt attgccaagg
     1021 cacagaagca tgatatgtca aatcaaatga aatgaatgaa acccaatagt gggaagctgg
     1081 agggtttcct cttttttttg atgatgttta gcgggaaatg acctataaaa atgcacgggt
     1141 tagcaaaaca atgtaatttc attataaatc ctaaagataa gtattctatt cattatttct
     1201 gtgatcatat ctcatcacag ggctctttat tgctgctttt gaggcccagt ttgtacagag
     1261 cgtcagggtt gagagcggga taatgggcct ttaattgcaa tgtggatcag gttaggaggg
     1321 gtggcattct cccagtattt atccctctcc atgaaatatt taatggtgtg ccgggagaaa
     1381 caaactctac acacattctc taattcataa cctggagata gccttagaca taagaagaca
     1441 atgcatccat atctcgatcc ctcctctttg tctctcgccg gctcgggctc ctatta
//
LOCUS       KCVB01000311             797 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14374pri ultra-conserved element locus
            uce-190, sequence.
ACCESSION   KCVB01000311
VERSION     KCVB01000311.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 797)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14374pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>797
                     /note="ultra-conserved element locus uce-190"
BASE COUNT          227 a          144 c          184 g          242 t
ORIGIN      
        1 tttctttacg cgacagcatc tcaactggaa tcctgggagg tgaaatgact ttatatcgtg
       61 attatatttt acaagccaca gtaaaatatt cccaactaac attattacaa gcaatgaatg
      121 ggaagtagtg taaagggtca acacctctgc aaccactgcc gagtcaatag atttaataaa
      181 tgttcctcgt tccccgtaaa atgctcctat aggagtagcc ggtgtgttta ctttacaaag
      241 caatacacgt gagtaaatgt cctttccaca ctcataaact catctccaga gaagcactta
      301 ctgggcattg tttggccgca agacttaata tacaaatgag aaaaagtgta tatgtgtaag
      361 ggtgggagat acacagagac agacacacac acacacacac attctccaca gctggaataa
      421 ctcttcgcag actaatgtaa ttaatagctt tttatccatg tagaatttga aatgaagatg
      481 taaattgttc gtttaacata gccataggac atggaggctt ctgctgtcaa agtcaattac
      541 agactcagca tagtcccaga ctttatgaaa acctaatcaa gatgtggtct gtgtgtgtgt
      601 gtgtgtgtgt gtgagtgtgt ctggacacaa acttgagaca tggagtgtga gcctgtacgc
      661 atgcattttt atgtgtctgt gtctgtgtgt gtgtgtgtgt gtgtgtgagt gtgtctggac
      721 acaaacttga gacatggagt gtgagcctgt acgcatgcat ttttatgtgt ctgtgtctgt
      781 gtgtgtgtgt gtgtgtg
//
LOCUS       KCVB01000312            1643 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14412pri ultra-conserved element locus
            uce-229, sequence.
ACCESSION   KCVB01000312
VERSION     KCVB01000312.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14412pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1643
                     /note="ultra-conserved element locus uce-229"
BASE COUNT          547 a          306 c          273 g          517 t
ORIGIN      
        1 aaaggtggcc aataatctca ttcacacact gtttccatca cagaccccca tgtaccctac
       61 ataagaccat tgaagtaata ttgacattgc tcagctgcat aaaaacttgc atgtcataca
      121 caaatattat gaaaagttaa cactttcctc caagatatat gcacttatag ctgccactac
      181 aatggaaatc tccatttaaa cccttctgcc agacagtggg aatatgtgtg tttatacagt
      241 acttgcatag aaatatcaat acaatataca gaccaaaatc taattttact attacactgc
      301 tgatggcttt gagatattga gaaagtatgc tgagcataat cagtcaatag acctggtaga
      361 gggctgcatc acacatggta ataaagccac aacttcagca tggtgagctc attcccatag
      421 taccccccat ccaaccccca tctccctgca ccaatgcctg cctcatcact ccacacaaaa
      481 tatcccaccc ttaattggtc tgtgctcctc caacatgttt gtaatgaaaa agcagcacat
      541 aattcttagt ttgttggctg gcagagccca atttacaaaa gtggaggttg gtgataagca
      601 gagacagtac ggctgtggag tgctcagaac aatgaatcac cccactgcca gggttgagca
      661 gaatataatt gagtccaaca tctctgagca ctttcaccat aattaatgat aatctgtaac
      721 agaggaatct gagattccag ttcacagaac tgtttaatta ggcccagtaa tgaaagcaca
      781 ggcactacaa gaatatggcc ggcctcatga ttaatgattt gtgctactga acacaaaagt
      841 gctgcaccgt taactccaac ggatgctgtt gtgtctccct ggcatttaca gaacaccaaa
      901 atacatatgg agctgtttgc ttcccctata tgaaacagga taaatattct cctgttttca
      961 ttatttaata ctctggattg gccaaaacat ctttaacctc cagggaaatt actacattta
     1021 aaaaaataac tgataaatat atatttttta catgaaaatt aagatattta tgattgtctg
     1081 tccatatatg tatattaata tattgctctg gtcagtgttg acagttcagt ttagtctgat
     1141 ataaatgtcc aacagtgccc ctttaatgaa gttataaata gaacatgcta tcataggaag
     1201 tagatttgtt aattagatgc agatttgatg atatgtcgct tatggaagtt caataattgg
     1261 tgttttatta gattatgata aggaaacatt taagtatgat aaaatattac ataaaatcaa
     1321 atgtatttaa gcattaaaaa cgatgattag tcaataactt aattagtcga tcaagagaaa
     1381 attagttagc aactattttt gtaatagatt aattgtatta accttttttc aagctaaaat
     1441 gtcaaacatt ttctagcttt tcttttcttt gtcatttatt atagtaaatc aagggtcttt
     1501 aggtttcaga ctgttggttg aacaaaagaa gcaatttaaa gacgtcactt tgggctttgg
     1561 gaaattgtgc caagcatttt tcacgttttt tgacgttttg taaactaaat cattaatcag
     1621 ttattgatag tgaaattaat cat
//
LOCUS       KCVB01000313             667 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14458pri ultra-conserved element locus
            uce-89, sequence.
ACCESSION   KCVB01000313
VERSION     KCVB01000313.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 667)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14458pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>667
                     /note="ultra-conserved element locus uce-89"
BASE COUNT          184 a          107 c          234 g          142 t
ORIGIN      
        1 cacacacaca cgcatacaca cacacacata taatgagcca gtgcgttgct ggcagttttg
       61 tttttccagt ctgtacctct tctcccatta ggcccactgt gaaatcttta gtgatgtttt
      121 tgtgtgttag ccgcacagtg gaaccgctgc acagggtcat ggtacaagtt gtataagcag
      181 tagagggatg gagatggggt gcgggtgggg gaggcgaggg agaagactgg gtataaggac
      241 atgattatag agaggagatg tggggtaggt cagaaagggg ggaagaaaaa tcaaaggtag
      301 tttggatgag ggatcgagtg aaaaggggag tgaaagaacg caagaaacac agagagaaag
      361 gagaaaagaa aagggaaaga tggcagtacg gctgtgttgg caggatgggg gtgggtgcct
      421 atcggcggct gctccttcct gaaacgtggc cacccgagcc tgcctgggcc ctggaagcac
      481 atgtggaaca gtttactgta atggaactct aaattagtgg tggtttgaag ctgcctgcag
      541 cagtagggtg taattaactg gctctgttga aagagaaaga gccagaaaga gaggcaggga
      601 gggagccaga gccctggaaa aggcaagggg tgagaggtgt gtgtgtgtgt gtgtgtgtgc
      661 gtgtgtg
//
LOCUS       KCVB01000314            1680 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14502pri ultra-conserved element locus
            uce-371, sequence.
ACCESSION   KCVB01000314
VERSION     KCVB01000314.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14502pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1680
                     /note="ultra-conserved element locus uce-371"
BASE COUNT          459 a          339 c          331 g          551 t
ORIGIN      
        1 actaaatcta actaatcgtt agaggatcga gataaattca cagtgcttat gactgaattt
       61 ttattatttt acaattttgt tccatttaaa tacaacaaat tggacttgac ctgtaataat
      121 agtttattat tttctcaaat gtaaggattt gttgcatttt ttttgtcata tatgataata
      181 aattgaatat aatatgggtt tcagacagtt ggcctgacaa aacaagcaat tgaaaaacat
      241 agtttgaggc tttggataaa tataatgagc atttcttgat attttttatg gggtaaaaaa
      301 tgataattgc acaaaaaatc ggaaaatcaa tccattatca aaataattat tagttgcagc
      361 cctaatggga gctgcagtat ttttactttt gactgtgagt tttaaaacaa gagatcatta
      421 tatattaaaa attctgccct aattatcact acatattttc gtcctcacca ccactcatag
      481 cctttcatga aaagtttgca tcgcttttaa cttcctgtca tatgacatat agtagctata
      541 tatatacagc tctcttccgc tatgtgtcat ttatatagaa gtgttttttc tcttctaagt
      601 ttacatatca ggacacaggt agttaatgca cagatagcct tttcctgtgg cactgtgttt
      661 tacaccttgg cctcccgatg cgagggtgat agcatgtggg gtgtttgatt tctgcgccag
      721 gccgtaatga agtttgacat ttagtttgga gagagcagca gagcgcgatc aggccttgat
      781 tagtacgctc taattgagga taataagggg gaggcggtga ttgtttctaa tttttgccat
      841 taattgcagc cgttggcttt gattgaggac aggcagggct gccatgtcaa ggggcctggc
      901 ggaatcatag tgctcctcac ccggagcctc ttgaataaca atgaaatgac tccccccacc
      961 tcctcctctt catcccaccc tctgcctctc tctctctctc tctcaccctt ttttcattct
     1021 cattcattct cagtgtggca ttggtaaagg caggaagctg ttagcagccc cctctttgtc
     1081 tcacccacag tcttcatcct gaagcatgtt taaactgtca tctccgtggt gggagtgtaa
     1141 tacactgtaa tcacaggtta ggctgatgcc tgttctgctg cccaggtgat ctttgttgag
     1201 tgggaaagag actcctcatc cacagtgtgc ttcattgccc agtttggtcc ctatggccct
     1261 cgtacacgct aacagctagc taataacgcc actttgaatg cagattgagt ccaattctaa
     1321 cttcagaatt cccaacttaa gaaatataat ttatcagtcg tgcatagttt agacctgaga
     1381 accacttaaa ggcaggagac tttatattat acagaatcat tagttttcag gtgctttgat
     1441 attattactc agtgatgtga actgtaattt tatccatttc ctgcttctat accttcacgt
     1501 taaaagcttc ttaaagtctg attggtgcaa acaaaaggag gtatatagtc ctgtataagt
     1561 ttcaggtgca ggatgagcag ggccagggtc attttccaca acattagggg cagaaattga
     1621 taattagagg tgtttatgac acggttgggg cccccgaggg gcagccctcc cctttaacct
//
LOCUS       KCVB01000315            1631 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14546pri ultra-conserved element locus
            uce-334, sequence.
ACCESSION   KCVB01000315
VERSION     KCVB01000315.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14546pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1631
                     /note="ultra-conserved element locus uce-334"
BASE COUNT          527 a          255 c          324 g          525 t
ORIGIN      
        1 atcagtggaa aactgattca catcttagca ttgtccaaga gtgaaatttt cttaaagcct
       61 atacttcttg tactgtttaa tatcttataa agggagtaga tgaaccagtg gggagaagat
      121 gggaattcag gccacttggt ggactataca ttgaaaggaa tcaacaactg gctgccttat
      181 tcccaaagtc caaggcctta tgccatctgt agagtgactg tatatagtat aatgttaaga
      241 atcttcggaa ccatccagag cttcatcata tcaaggcagc tgttactctg atattttcag
      301 tactatttct aattgttttt gtttagcagt gttacaatat agatgcgtat tgtataggct
      361 aacaactaca ctgtgtgtgc ccttatcaat aatggattta gaggaatttg atatgctgtc
      421 atttcatgaa aaccatggca ggcacacttt cagtcatgag attttttttg gcatgctata
      481 ctcccagctt ggataagaat atcaatggta tggagaaggc ttagttacat tgaaggctag
      541 aggctgacat gcatgttttg acgtcatctt gaaatcaaga ggaaatgaaa ttattctgtt
      601 tgtggagaat ccagccttat ttccatacca aagatgatct gtttgaataa cataagagat
      661 tattacacat agagacactc ctagtaaaca ttaacaacaa cgggaaacgg gcagcctctg
      721 agatataaaa gagttttatt gcatttgagg aaaaggatta agttgcataa atcttatgta
      781 cagtatgtaa atttgtgaaa tggtcactgt cctatgtttt atggacctga aagcatgttg
      841 aaaccatcac tgctagccag gtaggctctg tgatacagta ggatgcttgc ttgagttttt
      901 ggctattgtc aatgttgtaa aagtcatgtt taagctccat ctgtaaatag gatagataca
      961 tagagatttg atgtatcaag acactatatt atggccatta ttctagctaa gtacgtgcca
     1021 ttttaaaaga aaaaaaagct taacagatct atagtcggca agatactgta tgttgtgtgt
     1081 tacatttctg gctcaatgtg tcgtaaaagt aatgctgtct ggtcttgctt gctgatttta
     1141 ttttactttt ttcttttcaa agataatagg tctctgcggt tgcagaagtg atgtgtgctt
     1201 tgagcaggat caatagcatg gtatattgag aaaaaaagtt gtgatactgt tagctggttg
     1261 caaacatctc tgtgacagac attgtgctca aagcctttca gcagacactg gaaaaaaaaa
     1321 aaacgattac tcatgtttca tcaaactttg gaggttcaca ttattgcact aaatttttat
     1381 gacacatggc tttgccacat ctttatggac aaatgtaacg atggatgccc acaaaaaaaa
     1441 gaaaaaaaat attttttatg acaagatgag tagggaaatt aactgtcaga aaatgactaa
     1501 ttgtcttgta gcaaaatgtg cattaatctc agtgagatac acatttctta ctcattctca
     1561 tacatgagaa tataaagcat tgagggaaaa aagttaaata atcttttaaa acctacatgc
     1621 tcattatgtt g
//
LOCUS       KCVB01000316            1400 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14626pri ultra-conserved element locus
            uce-1042, sequence.
ACCESSION   KCVB01000316
VERSION     KCVB01000316.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14626pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1400
                     /note="ultra-conserved element locus uce-1042"
BASE COUNT          378 a          297 c          340 g          385 t
ORIGIN      
        1 cgtaggaacg taacagggac tgcaactgaa tgctgagtgg ctgacgtgaa tatctggctc
       61 ttgattgaca gatgttttat tttggtacaa tgttctcatt gaggggtccg gtgtgaagca
      121 aggctggggg gttggacagc agggatttca acatgattaa atgccccctc tcagagagat
      181 ggtttcacaa aggcaaggac agacaaactc catcagctag aagcagggag acaaaaaatg
      241 agacttaaat tagaaaccaa agcaatctga gctaaaatag agattataag agcgtaaacc
      301 accataaaag aacatatatc ttctaaagtg ctgaattaaa ggaatccaca ttatgtcgag
      361 agctggcaaa gagtaaaaca ggttgaaatt ttgaaatgta tgtagaaatc aaatggttga
      421 ataacacatg atggaatgaa agctgcaagt gtttttttta ccttcttttt tcagcagatg
      481 acttaatttg cacaggaaaa gctgggctcc ctctgaaggg ctacctccct ccctctctct
      541 ttctttttct ccctccctcc cttcttctct ccttctctcc tctttggact gtaccgtgac
      601 cttcattggc gttccacctg caggaagcca tgtttgattc tctgtccgcc gagacgcctg
      661 cttgacagca gaatggtgtc ataggacaga gtggaggaat ttctctttct ctgtcatcat
      721 taaggggctt ccccctatgg tgagggggat gaaaagtacg atttaatgtt ctctggagag
      781 tggcagcaga agaaaagctc aaagccctgc atgctggttt ctgagtgggt cactccagcc
      841 cttgcagcga atgggatgtc ccttaggaat ttgccataga ccagcctgaa aggctaggga
      901 ttaagaccct tttaaggatg gaaatgtaac aaaaggagca cttttatgat gtagcagcat
      961 aaaatgaggg ggtccattgt gtaactgatt gcaaaagatc tttgaaacag gcggctctgc
     1021 actgcatgct cgcctggccc agtgggcttt aacggcttgt acaaaatgac ttgtctacat
     1081 tagggacaga gtggctctcg tttaggaggc aatgtagaag attattggtt tgtttctatt
     1141 ttcacccttt gctcctccct ccctttccct ctctctcact cactcactcc ctcctctccg
     1201 tctgctatca atgctggatt gaaagtggcg acagagtccc agtgaaagtt ggttaattta
     1261 gagcaagaga gaaggtaatt agcatgccct cgggcgtggc gagactagaa taggaggcac
     1321 tagtatagct tagatgtgaa tgggaaccac cgtgcttggt tttcaggcaa tagtgaccag
     1381 atgctgtgac tgtgctcact
//
LOCUS       KCVB01000317            1482 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14660pri ultra-conserved element locus
            uce-718, sequence.
ACCESSION   KCVB01000317
VERSION     KCVB01000317.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14660pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1482
                     /note="ultra-conserved element locus uce-718"
BASE COUNT          418 a          311 c          299 g          454 t
ORIGIN      
        1 ttagctggtt actcgtaaaa ctgagcatgt cttgtcctct ggatccgcta catgcttgga
       61 gttcatccct gaaactttga tgtggactga tttaagggat tacaagtaat tcctaaaagg
      121 atttagcatt aatttcgaca tttatttatt atttataaaa agtctggtcg cttgattatt
      181 tatccgttca tccagcattg aaagtggaaa atctttagaa caagaaagtc gatttcggtt
      241 ttcttttagc gcttatatta ttgttttttc cccatttgcc tttttttctc tgcagtgcta
      301 aagtgagaca gtgaggctct attcaaaggc aaaaaaaggg ctctggcaag gacaacagtt
      361 ttatccctgt cttcataaca aaacacaaat tctacataaa ttccctctct aagggctctt
      421 taaccctttt ctccttcccc atgctcaatt aggtttgctt ttattattta tttattgtat
      481 atttaaaggc aactaacact cggaagtgta ataaaatgta cagtgggggg attaggagca
      541 gctctttaat ctcacgcttc tgataatact gcatgggctg attgattagt ttttcaggaa
      601 gcttatgtca gaaaaaccta atccgtcttt ggattgtgcc agccttgtaa ggttttccgc
      661 gctgccactt tatcatggcg gcgaattaca gagctgctgg gctgtgataa ggtcctcgtc
      721 tcttttacag accccaccgc ccaaccccct gtacccccac ccctttccct ctggacaata
      781 aaaaaaaaat tgtgtttata ttcttcacat ccctgctctg cctggtcttc acatcaacct
      841 ctcccaggat ttgccttgta agaaagagag agagaaaaaa gagtggaact taagtcttca
      901 aaaacccacc agagaaattg ggtgttgaga gagaaaaata tgagagtaaa tggcagacag
      961 gcaggccagt agaaaattct gtggaagtga ttaaacatgg attgacagag tgaaggggcc
     1021 tcgtatgcat gtgctgcctt ccaggaatca ttttggagct tacatgtttc cgatgtgtat
     1081 ctgcgcttat attggggtca cagtgttttc agccagaggg cttgactaga taatccccca
     1141 taaacaatac tgtctgtggc atttgcagcc ctcagcctgt atgttgtctc agacctctga
     1201 aggagacctt gtcatttctg cgggctaaat ccctcaaatg tagctcgcag acaccacagg
     1261 ttgtattgac cgtaatgagc cctgcaacct attccatttc caatcatcaa tcataattga
     1321 gtgtgtgctg gcaataaatc tatggtaaag gtcctgaaat gaagagaaac aagctccaaa
     1381 agatagatag atgtgacaca tttgcccttt tgcatttaca agaatcataa tctggctact
     1441 ggatggactt aagtcggaca attatgagca gcacaattac aa
//
LOCUS       KCVB01000318            1074 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14741pri ultra-conserved element locus
            uce-74, sequence.
ACCESSION   KCVB01000318
VERSION     KCVB01000318.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14741pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1074
                     /note="ultra-conserved element locus uce-74"
BASE COUNT          395 a          182 c          183 g          314 t
ORIGIN      
        1 aaaaatgatg ctaagtgaat actaatgttg ctctgtatct attagatggg ttgggcagct
       61 gtttgttcag acattcacca tatcaaccgc atatgatttc aaacgtgtta tgaataagct
      121 atgaaactga ctaatatttt gccatgtttt ttggaaaaac atccacaaaa cacaaaacat
      181 gatatgcaca tttctttatt tcataaagag aaatggtgtc tagacaaaaa agtaatttat
      241 agaacactag caattgaaat gaatgaaaat ggggtttgca caatactgta tttacaatgg
      301 ttattaagtc attttaatta attagggaca tgtagcattt aaaaattata aaggtaagaa
      361 ctcttagtag agaataagca aataacagct atttcaaatt gacaatatta acacaaaaca
      421 atcatctata aaaatgaaag aaaaaaacaa caacttgaac tacaagctta agagctatta
      481 cattaaaaat gatgcaccaa aatctactaa tacaataatc catataatct gtgtaaaaag
      541 catgtagaaa aaacacctga tgcagtttgc tgatacagtc attgaccagt tatgagaggt
      601 atgaaggatt tcacaggtag ataagtgtga gaaagtccgc actggtgact cagggatggg
      661 ggcccaggcc cctgcataca ctcacttcac ttgaatgtga atgatggcct ctgcaagttc
      721 taaggcaaat atcggaggtg cacttgtatc catgtaatgc tgacccatcc caaaaattgg
      781 attaaagaca aggtgaggaa actcattctt tgtctctttt cacaacacca agaaccagtc
      841 caattgaacc gagcatcaat tttacatggc aaaaactgat caaacatgac atgacaccta
      901 tttgtaaaca ttttccattt ttatatttta ctcaatcaac tcagcctgcg ctttagacca
      961 ggataccaga aaaatttttt tttttcaata aagttgagga atctgtgaga aaaaggcatg
     1021 cttttgttca tattccttta tagaacaacc tttggagttg acaagccagt gttt
//
LOCUS       KCVB01000319            1630 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14787pri ultra-conserved element locus
            uce-31, sequence.
ACCESSION   KCVB01000319
VERSION     KCVB01000319.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14787pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1630
                     /note="ultra-conserved element locus uce-31"
BASE COUNT          487 a          299 c          400 g          444 t
ORIGIN      
        1 gacttcagtt cagcatttca gctcatcaac atttaactaa tagaactaaa aaaaaacacc
       61 cataaatggt tcccagtgtg taatattcat tgttaagcat gttgctacag tacattcatg
      121 tatttgtgca agttgtgtgg gtagctatta taccttatca ggatttctgc aaaacagaac
      181 agtttggtaa cactgaaaat gaaaagatgt caataatttg gtatctgaga aaaactgaag
      241 ccaaggctgg ttgtgcttcc attgttcagt atcagctctt ggctcattat tgtgcggaaa
      301 tgtatttcat aacatagttt tctcccaaac cttcctagct ttcacaccaa tagtcctctg
      361 atatgcaaga gaaaaaaaaa catattattt caatactgta ttcattttgc attaaacaag
      421 gcacattcat tcaagacata ttttcagtct gtgtctctcg cacaaatatc cacagacaca
      481 cacacaaaca cacatgtgaa catatccata gagcggggct gaaaaaacaa ttacagtttc
      541 tcattaaggc actcctaatc cctttatgca aaacacagtt acacaagtac acacaatgag
      601 gaaaaagaga gaatgaggga ggggaggaaa gagggaggga gagtgaagaa aaaaaaattc
      661 aggcaaatgc gaggagaata attacatata tcatgaagcc gtccctttta tttcggatgt
      721 cacttctgat gtcactggcc ggctttggcg ctaatttgaa ttcagacctt tatcagtcgc
      781 cggcagcaga ttttgagtga cagcgccacc aaacgcctct cgcccaacgt cctattgatt
      841 tctctaaatc cccaacaata gcatttgcat gcttgtttct gttataatac agatatgccg
      901 tttcggaaat taaagagcgg caggcagacc agaggtaact aattgtgtgc ctgtgtaatg
      961 agtgcagttt caatttgata taacccacag cttttcccct cgcaggcccg cgctaagtac
     1021 tttcaagtct ttgtggggaa ataattgatt tttgtgcttt gggcacaggg aaaactatta
     1081 tgatcttttt gaataatggc agcgggagga aaggcacgtt gaacgattta aattggtatt
     1141 agaggagagg aaggaaggaa aggagggctt ggtgacgggc ggaggactga tagcgtagcg
     1201 gaggcctgga tttgactgac agtcggcaac tggctcctgg tttgttgatt actcattctc
     1261 ttaaggtgga agtggggcaa tgtgaggtgt tgttacggca gtaagtaaag ccacattgag
     1321 cctgactggt tgaattgctg gctcagggaa aggagaagac ggagggatgc tataaggagg
     1381 gcggtcaggg tggtctggct ggctagcgat gccaggggcc tgttagctgg ccaaaacagt
     1441 gggtgaatga acaagcacac agggtggggt tggcacgtcg tcgtatagct tcagggtgtt
     1501 gcgtgtgtgt gtgcgtgtgt ttgtgtgttg acgagtgcat ctttaaacag gatggtgaaa
     1561 ataaaaagtc agtgtgcaaa gtcatagaaa atgcaacact ctcctgtgga tggttataaa
     1621 cctaaatgtg
//
LOCUS       KCVB01000320             307 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14820pri ultra-conserved element locus
            uce-679, sequence.
ACCESSION   KCVB01000320
VERSION     KCVB01000320.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 307)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14820pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>307
                     /note="ultra-conserved element locus uce-679"
BASE COUNT           56 a           42 c          108 g          101 t
ORIGIN      
        1 ctcagtttga gatgcgtgca tgagtgtgta catgtgtgtg tgtgtgtgtg tgtgtgtgtg
       61 tgtgttgggg gggatgcgtc catatgaaat gtacacgagg gtgaatctca gtggtgttaa
      121 tggcttttta aatgtcaggg aaagtcaggg aatgataaag gagagatctg ttatccactg
      181 tcctcatgat caccgcggag cacgccactg ctctcactcg aggagcctca gtttgagatg
      241 cgtgcatgag tgtgtacatg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      301 tgtgtgt
//
LOCUS       KCVB01000321            1508 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14861pri ultra-conserved element locus
            uce-1166, sequence.
ACCESSION   KCVB01000321
VERSION     KCVB01000321.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14861pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1508
                     /note="ultra-conserved element locus uce-1166"
BASE COUNT          440 a          373 c          304 g          391 t
ORIGIN      
        1 taattcaatc caggagtcca cttgaatgtg gaatctgagc caaagtaagt tacttcactt
       61 tctcttctgg tccagagaaa aatcataaat aataaagaca tgcaagcgcc catatcgctg
      121 ccttatctta ccaaacaaaa cctgcagaca agctgaggag gctgtaaacc actaaggaga
      181 cataaaataa ataaacaaca ataataacac ttaggtatca cagcctgtac ttaatgttta
      241 tcaggtgtcg tggcggccat gacatcgtgc tgagtaagat tctctacagg aggcatcctc
      301 ttggcctcgt cgccagcatg tgggctccga cgggagctga aaaggcgaga caagagatta
      361 aggaggcaag aagctttgtc tgtgcaccct ccctgcctca aacacaacaa cctctccata
      421 caccctcccc ccgcaacaca cacacataga ctctgctctc attttttctt attaccgtaa
      481 gcatggaggg aaaaaaggac aaaatagtac ttactaatgg actaaaaatg tcccaactcc
      541 ctaccctttc actccccaac agccagaaaa accactcacc aaatctgttc tcagtgtaac
      601 tgtcgaggat tacatttagt caatcataca gtgccctata aggaatggat tttgggacat
      661 cttgtgagac tgggtgaaat gtgagttaat gggaagagag tctgtggttg agtgaagaag
      721 ccaggcagag taggtagtta aagagcagaa cagtgcctta ggggtactca gtgtatccgg
      781 actcacactg ggaaaagtgt atttatcaac ttaatagcag gtgtctgatt gaacatgact
      841 atgattacag taagttgttc cttcttttgg ctcctctcct cctcctcctc atcatactcc
      901 ccctcaccgc tccctcctgt tttccaccta cctacccttc ttttcaaagc tacaggaaaa
      961 tggtatggta ggtattatca ttgtcaacac taagtgattt aattatatta caacagcata
     1021 atgtattaaa ggcacagatc atttataatt cagtttacga ggcctgtcaa caacctgcgt
     1081 gtgcggcagt gttctcagaa ctttctcgcc gttgctgtct tctttgtttt taatgatttt
     1141 ttcaaagaac ttttactatt tgctttattg caaagtgtaa catccaaata gacagacagt
     1201 tcaagtaaac cttatctgga aagaaatctg gaatatcaga agcaggagtg atattctacg
     1261 agagaaacaa gcgaataaac aagatgagaa atctggcttg tggaagatgg ggagcaggag
     1321 ccatttagct gataaggcag ggaaggccct tcaggtgtct cctgctccac tgctgtgtaa
     1381 atgagctcca attaaacagg cggcccctgg ctcagggtct ccccgcagcc ctccccagcc
     1441 tcctcagcct cctccatccc ctaagcctcc cccagcccct tgctgctcgc cgtaaggccc
     1501 aacccacc
//
LOCUS       KCVB01000322            1416 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14907pri ultra-conserved element locus
            uce-1123, sequence.
ACCESSION   KCVB01000322
VERSION     KCVB01000322.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14907pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1416
                     /note="ultra-conserved element locus uce-1123"
BASE COUNT          396 a          280 c          325 g          415 t
ORIGIN      
        1 tccaacccca ttccctgttg gccccatgca gcgtgtgcca ttttccattt tcccttactc
       61 attacaggca gggctgttga ccttgtgctt ctggttcatg tacaagagca gatgtgttgg
      121 ctctgtgggg cttaatagag agcagcggta caagctattg tctgttgagg tttatcagtc
      181 gttagcctgt tagcatgcta aatgtgacaa agatttccac agcggtttac ttgcaaaata
      241 aagtctgtga aagcgcctcg aacaaagggg aggaaaagaa aataatttga ggaggagaag
      301 actttagtgt ctttctcaga gcccctcgag tatgtgtcta gcctggcccc cacgtctccc
      361 tgtaggacac agaggtgact ttggaaagtg atctgcacac tgctgggctg gtctcaagtg
      421 tcgtcatggc gagttaccgg gcaaatggat attcgcatcc tcatgaaaac caacaaaggg
      481 atatgtggtg tatgtacata ttcaactttg tatgttcaag ggtgttctgc agcggagtga
      541 gtctgcgctt caagaatcaa tctttattaa aaaggataag actgaagaat tgatttaatg
      601 aatgcggcct catgtcctgc agccacatgt aatccgcaga gaatcattga gcatgagctc
      661 agggggacaa aaaatgttcg tagaagactc atagagtggc ctgtaatgat agtctccata
      721 taatcccata attccttgtt taattaaact ctggagctgg ccttgagact acgccagctc
      781 tgttgctctt ttgttctcac agttgttatt tggaggctag gtttgagtat tcttttgtcc
      841 acttatgttg aaagatttga ctggagtgag ctataaacag agaccatggc tgtgaaacaa
      901 taaattagac ggtaggaact cactcaggcg agacattcag gtgagcatta tagttttcat
      961 ttgagctgaa tagcagaggg cctctgtcag aggcagaaat ctcttcatac accctcttct
     1021 tctatatctc cctttatttt ccttctccca cactttctgg actgagcatg atgcaatcca
     1081 actaggcctg gagcaacact ggcacatcac gccgccttgt caatgtctca atgaggaaat
     1141 tatgaaaata taaattttat gacatgaaat gaaaagggcc atgtagtttg aatagacatg
     1201 cagaggaggt gaaaatgatg caattttcta aattcagtta taatggtaat gtgcaatggt
     1261 aatgcgtatt tgctgtcaaa aatttccctg tcggcccgga ttgtacagct ttgtgctgca
     1321 tgtatattcc tcgagcaaaa attcagctta gttgaatgcc taaaaagtaa tgtaagatat
     1381 gttctaattc atttaatgac agcagatgga aacaca
//
LOCUS       KCVB01000323             456 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14950pri ultra-conserved element locus
            uce-111, sequence.
ACCESSION   KCVB01000323
VERSION     KCVB01000323.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 456)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14950pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>456
                     /note="ultra-conserved element locus uce-111"
BASE COUNT          128 a          109 c           98 g          121 t
ORIGIN      
        1 acacacacac acacacacac acacacacac acacacagca ggagagaagg acacggactg
       61 ctcagtatgc agccctgaca ttgtccacat ctgatacggg acattagccc ctaactccct
      121 ccatacaccg ctgcccttca ctttatatta ctttatgtta atagaaaaat attacataca
      181 acatcatttg tctttgttac cacggcaccc cacagctgat ttaaatgcat gtgtttacct
      241 ctacagacag gtgaagacag ggggaagggt gggtgacgga ctgtgtgtgt gtctatgcat
      301 gcgtgtgtct caatgtgtgt gtctctgtaa aaggggaggg tacaaacaca cacatcttgt
      361 tcagatttat cgatcttacc cacctctcac caggatcatg ctgtaacata tgtactgtat
      421 ataaaggcat agagtgtgtg tgtgtgtgtg tgtgtg
//
LOCUS       KCVB01000324            2023 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 14994pri ultra-conserved element locus
            uce-952, sequence.
ACCESSION   KCVB01000324
VERSION     KCVB01000324.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="14994pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2023
                     /note="ultra-conserved element locus uce-952"
BASE COUNT          455 a          454 c          392 g          722 t
ORIGIN      
        1 gaacatagtt agtatttacc ctgcctatca tcagcctaac ctgcctgttg gtaggttaat
       61 gtgagcatgt tagcacactg agcttagcat ttagtttgaa gtacagcctc acagagcgtg
      121 gctgcatgcc tgtagactct tgttacaatg acatttgtgc tgtgggaagc caaggaacta
      181 aaaaagcttt cctgtggtca tgagggtgag ggtcatgctt tgattacaaa gatcacattg
      241 tgctacactt ttgtggaaag tgccaaaccg tggatcagcg taataaccaa gaaaggaaat
      301 caagtcacat aagtgtcaaa gtgagggaaa ttttagaatt tcatactttt aaatattgct
      361 gtggacagga aagaccaaaa ggagtgtcag ctccaaaacg atgactcatg aacagcttcg
      421 gcttacattt atgagttcct tttcctgtgc cagcgtcctg atggaccctg ggtaaaagaa
      481 caccagtcgg aggcttggcc tcttgatatt tggggctcct gtcgaagcca acggcagcaa
      541 gttttgtgcc tccagatttg ggagttttta atgaagccga gcagctgcag ttttcccttc
      601 agtctggaaa cgagaggctt tgtgtccctt gtcaccctgt gttaatttgg gccctaatgt
      661 attttataaa caaagggctg ctctctagcc tgacagtcgt gacaccttga ttccagagca
      721 ggatgcatgc agtacggggt tgcaccatta ataatcaact ggcagccatc acaccatttt
      781 aaccacgatc attatgataa agggcataca cttaattaca ggcctgctta attatagctc
      841 tgttgaagct cgtttgtttg gtcaaatgct aattatacca ccacgctctt ggcaagtcct
      901 catttcaatc cactttttgc cggctgaaca gtgtggctta ttttcatgca aatttgatat
      961 ctggcgaatg ttttgtctct cttttttccg cctttctgtg catagtgtga gtgtgtcttt
     1021 gtttcggagg tgaacatctc tcagacaaga cggaatcggg aataatcaag agtaattaag
     1081 ctggaagagg gttgggaaag ggctgggttg gaggagagag tggaggtgct caaatacagc
     1141 agagaataaa aaagcctttc atcttctctc catagacgga aggaggggag gtgtcagggt
     1201 ggttggggat gttgatagct ctgctgtttt ataaacccac gtttagtctt gtggaaatac
     1261 acaggattaa gaataggaag gaatatatat atttatatat tttgtatgaa agtcgaattt
     1321 aaacttggtt ttctcttaac ttttttgcag ctctggatga tgtgcccttt gtgatctctg
     1381 agaagttttg tcaaaacccc aaaaaggtaa aatttattta aggagcttga aacaaaatgc
     1441 aaaattattg cttttttaca aataatgtga gagaggtcgt ggttactgtt cagtcacttg
     1501 tcttgtaact tgttttgtag aatgagcccc gtaaaggtta tgatatctgg tcctttttta
     1561 gacccttctt cctctctttt gctattcttt ccttgctctt ctttgttttt ctgtcctttc
     1621 ctctccctgt ctttctatta tggccctttt ctttgctttt ctcttcttgt ctcctcttct
     1681 ctcttccttt tttttgtctc acccttgcct cttattttat tttatttact ctgcatttct
     1741 tctcttcttc taaactgtct tttcctataa tctcatgtcc tcttttctct ttttaccgtc
     1801 tcctctcatt acccctttct tatcgttttc tctgtctact catttcctct cattctttcc
     1861 tctcctcatc accactcttc tttctgtttt ttctctcctc atttccttcc ctctctttat
     1921 atcctccact ctcctcattc cttctcctct cctctccttg tttccttgtc tcctctcctc
     1981 tccttgtctc ttctccttgt ctcctctcct tgtctcctct cct
//
LOCUS       KCVB01000325            1571 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15038pri ultra-conserved element locus
            uce-585, sequence.
ACCESSION   KCVB01000325
VERSION     KCVB01000325.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15038pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1571
                     /note="ultra-conserved element locus uce-585"
BASE COUNT          424 a          348 c          324 g          475 t
ORIGIN      
        1 agaatattcc tgctgtgaaa cagagaacga aaatttaaga aacgctttag aaaatgtcac
       61 gtggatcaac atttagtaca cagggttggt gtgcccatta aagtgagact gaaacacctg
      121 agtagcagaa aaccagctca gccctttctt ttaaaattat gagtatgttc ccccacacat
      181 atactaagac gtgtcatgac tgtttgtcct gcaatacatc atgtttcaca gagctccctg
      241 ttttaggata atattatagt aaacattttc tacagtgttc ttctctaaag ctgaaattta
      301 ctttctgcgc cctcaggggt ttgccagggt ccacgtgacc taaattcatc agagggtgta
      361 cgtgtagact ctgtgcagac atccatgtac ctctgactgc gcagacttgt ccctttgaac
      421 agtacatgtc aatcctgtag acattaatct actgtgtgaa atgcgtcctc cctgtggagt
      481 gcagaaagtg gcataaacag aggtactatg tgtctcaggg tgtcgaagct gtctgtgtag
      541 gtgtatggga gtgggagtat attcagtcct taagttactc tgcttgctta attgtatcac
      601 acacatctag cagtcaccgc tctactgcca attttttatt atctgtcagg ccacagcaca
      661 tatgttcagg atgttttgat ttgcgaaatg tttctgcctt gctagttgtt ttaaatgcat
      721 gcttgtacag tcaacacttt tactttcata ctgtatggta tactgtgtaa acctggtgtc
      781 aaatttctct ctctccctct ccctctccat tgcctcccac agtctcaggg ctcaccatac
      841 tcaatgagcg gacagcgcaa tgtcagagcg ctctgggcaa actgcaaagg ggaaggaagg
      901 caaaaccaag tatgcgtctc tcaacctgtt tgatacatac aaaggaaaga gccttgaaac
      961 acaaaagcct gttggtgagt atcatgggtc tgtcatgtcc aagctgtcat tgtcacaaca
     1021 tactgtatat ccagtgaaat gtattggtct ttcaaccaag ggtattcatt ttcaaattct
     1081 gctagatggt agtactgcaa ctctaatgca ttagcctact gtcccccgta atgctgtaat
     1141 gctgatttcc aatacaggaa atggataata tgcagcatct cttaactgcc tgccaaattt
     1201 aatttttgct gatactgcta atatataata tatattatag agggaccagg gatatttact
     1261 gttcttatct ataggttgtt gcacctgtct ctgagagcaa aagacatttc gattagaaat
     1321 tgtcacaact gtagccatat agagagacat tatgcaaggc ctggagggtg aatagctctc
     1381 gagccttggc atgtcagctg agccacagag ccaatgagcc tgacctgaga cagaggcttg
     1441 cttgtggacg ctgtttgact tctatgctga gtccccagtc tatgttttat tcagaagctc
     1501 ccctctagat ctaagcagct gtccctgttt ccaaactaca gacatcctcc tcctacatag
     1561 tttagccatg c
//
LOCUS       KCVB01000326             408 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15085pri ultra-conserved element locus
            uce-154, sequence.
ACCESSION   KCVB01000326
VERSION     KCVB01000326.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 408)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15085pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>408
                     /note="ultra-conserved element locus uce-154"
BASE COUNT          115 a           81 c           86 g          126 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtggctatc ctccagagcc ccttcccctc ctcagccttc
       61 gtctttcatt caacgccaag tcctttcaaa acaaggtgaa gttcagcaaa gtttctctgc
      121 agagatgatc aaacagtcac atcactaatg gcgatgggtg acagggccag tgaatacaga
      181 tcagctttaa ttgtggtggg tcttactgcc actgtctgtc aatctgagcc tttgaattgg
      241 tgaaaatttg ctctgttcat tctgttaaat gacgtatcca attaaatgct ttagaattca
      301 tgatcttcag tcaaggttaa atgaaagcag ttactcctgt cagtgaggtg attaaaagct
      361 gactacatca ggcacatttt gtgcaaaaaa aaaaaaaaat taaatttg
//
LOCUS       KCVB01000327            1975 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15126pri ultra-conserved element locus
            uce-917, sequence.
ACCESSION   KCVB01000327
VERSION     KCVB01000327.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15126pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1975
                     /note="ultra-conserved element locus uce-917"
BASE COUNT          683 a          321 c          400 g          571 t
ORIGIN      
        1 atattcagta tattagtatt tcttttacat ttaatacatt ttgaggcctt aaaacatact
       61 gtaaagtata ttttctgatt ggagactctt ctaaatggta aactattatc tgaaataaga
      121 ttttcttcta gaggataact taaaaaacta ggcttttttt ttgtttttta ttttatttta
      181 tcatgcaacc tctagtatcc aaactacaaa tgcttcattc agtacaatta agatgcaaag
      241 atggccaccc aacaaaacta tgatttttct ccaaaaaaca ctcaagcagc tgggttatgt
      301 gactgtatcc tccaatttgg cttctattga aacagtacaa actcattgca gagcaccaca
      361 acacaaacac cagtcaattt acccattttg catccaattc tctgggatga ttatgagccg
      421 cattgctgct tttgatggag agattaatct tgctttgata gaaatatgtc agtttctgca
      481 ggctgtgatg gatgtgattt atcattctgg acaaatctgt gcctgaatgt ccctcctcct
      541 gacagctcta tcgtggcaag aatgtcccaa aatgtcacct gttgtacgcc gagtgacctt
      601 ctaattttct aattcaattt cgagaactcc cctccccacg actcagccca tatcatgaac
      661 cagtaataac aattacacac cgtgactggc tccaaaacct gcaattcccc cactttaaat
      721 caaagcccaa gtagtctttt attaggctgc ccggttaaat caatctaaat ctaagcataa
      781 taactctcta atcaggtgct ggggagaata ataggttgtc acgctcgagg aaaaataggc
      841 aatcatggaa tgttttcttt aattaggccg gctaaagtag aagggcaagg cgtgagcgcg
      901 ggacacattc atcatagcca aggtgggaaa ttacttcaat tactcaggaa ggggagagtg
      961 aacagaatta cagaagtcag agaagcagaa gagaggagac cgtgatctgc aaggaagtcc
     1021 aaatgacacc gggggcagag gaggcaagga cagaggaggg aaggagggag gaaacaaggg
     1081 tgggaggaga gaggaaaggt agagtgaaag gaaaagtgga gacaatgcca acgtttcaca
     1141 attatgttca taaacatgca tatttgcatt aaaagaagag ggaaacaaaa tacgttaaaa
     1201 cttttttact gttattgtaa gcttatcatt ttttcacttt gtatacacat tttttaatat
     1261 gtctaagctt tcctttattt ttgtttttat tcaataaaca ttattgttat ttattatgtc
     1321 tttttgtaat ctttaattta gggggggttc gttgtataag gataccacat ctggacttct
     1381 aacaaaaatc taaagtcaag gtctatttac aagctttttc taaagtgagt gaaaactttt
     1441 tcacttttcg aaaactaatt tccatgctgt ttacataaac ataaaataat aacattttaa
     1501 aacctgagag aaaagatata aaagaaacgc agcttttggt tgcaaaatat attttaagaa
     1561 tttgctgcat tatttgagga atgttaattt ttcttttgac tgattaatta aaacattttg
     1621 gtgtgaaagt tacgacaagt ttaaaaaaaa caattcactg ccgacagagg aacatgaaag
     1681 atgaaataaa agtgaaagat taataaatga tcaaaagaaa tgaaataaag gatgaaaaag
     1741 atgatgaaaa aaattttaaa aaagagggag gacagatgaa agagggggag gacaaaaagg
     1801 agatcaggga gaaggtaaag gactgaggga gtgaggaagg cagaaagaaa gactggagcg
     1861 attgtgatga aatgaaacaa gcttaaatga cagcttggac aacagagggg aacctcggta
     1921 gccgaatgct tgcagcactt acagcatggt cgtttcttta attcaagccg gcgat
//
LOCUS       KCVB01000328            1398 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15166pri ultra-conserved element locus
            uce-578, sequence.
ACCESSION   KCVB01000328
VERSION     KCVB01000328.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15166pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1398
                     /note="ultra-conserved element locus uce-578"
BASE COUNT          429 a          280 c          339 g          350 t
ORIGIN      
        1 attttgatga agtaggcgag tgcttgctgg tgtgcttcca acacctagca gccagcacca
       61 caccacagcc actaccgcac cgccaccacc accaccacca ctgcccctta ctctatttaa
      121 acaattctgc tgagaagcag tttgaattgg aggggaattc agaatcactt ttagcatttg
      181 gcttaggaat agaaaaatct gacacttaga tttacactcc tctctggggc atcgtgaatg
      241 gttctctgaa gctcactcgt acagggaccc atgctgctcg tgtcgccaca gcagcacact
      301 ctaacaaatg atattaagga catcaatcaa ggcacttctt tgctcaaagt aatgagtaaa
      361 caagtgggag aacaaattga gaatatttag taggcaggaa tgggttgttt tggagaagca
      421 atggacagaa ctacatagta atcaaagctg gtccagtcag actaacaggt cagggcaata
      481 ccaagtttct aaccagccaa accttgccat aaaagcaggg atatctgctc ggagcgatgt
      541 tgtttatgag gaatttcatc ttttggaaga gcttgctatc cgttctacca tctgtttcct
      601 aatattcagt cagatgttgg agttgtatgt taataaacca agaagatttg gttttcagcg
      661 gactcactgc tgtatataat acaacccact gcgggattgt ggatggtagc agagtagaca
      721 gcttcaggcc tgtaaacagt aggtgggttt acttctctct tctctccgcg ggaactatcc
      781 attaacagat aatgggggga aaatcagagt gattaaggcc cagggaatta tgggtgaata
      841 caaattgact ccacctccgt gtggtgtgct ctcttgaggt agaagcactg ctcataagaa
      901 acaaactcat ttcagaagta ctacaaggcc cattaaggcc tggagaatgc agtggatggg
      961 ggtaggaagg tggtaggtgg gagcgcaaag ggatggactc cccctggacc aaagctgatt
     1021 ggagaactgg cgcctggggg gaacattact aatatctatt tcctgtctcc acagtttgag
     1081 aaggacagaa tgaatcaagt gaaaaatgta aaaatgaaag atttaatcat gcaccttgaa
     1141 aattcacccc attctatcaa agagttaatg tattacacga acagtgatgg aaatgtggtc
     1201 gagtgctagc taataaaatc aaagggattg gaaaactaca cagcatgaaa ggagaagctt
     1261 tacttgagtt tgcctgtagg gaagtgggca tgtaaaaggc atattttgtg tctgcaagag
     1321 aaaagtgaaa agtgaaagat aagctaagcg aggtaccaaa gccctcatgt cagggtccca
     1381 agttcagtgc gtgcctga
//
LOCUS       KCVB01000329            1933 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15210pri ultra-conserved element locus
            uce-1267, sequence.
ACCESSION   KCVB01000329
VERSION     KCVB01000329.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15210pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1933
                     /note="ultra-conserved element locus uce-1267"
BASE COUNT          620 a          322 c          531 g          460 t
ORIGIN      
        1 gtatgttaag tttgtttttc tttacacctt gccatattac tttagatttt ctccatttca
       61 tgaggagtgg agagctttaa gagctcaagg ttaaggggga aagataggga ttttaccaca
      121 ggacctgtcc tgtgtaagtg gtgaaagggg gtttaggggg gctggaagat gagaattaag
      181 ctggactggg tgaagtttta tgggggtgac aaggtgtaag gttgcagtta aaggtctgga
      241 ggaatttgag gagttgcccc cagactgcaa ggaaatgaca ttagaggtta agagctgagg
      301 ttaggatcag ggttagaggc tggtctccaa actgtgaaga gggctggcgg ggctggagga
      361 agcggctgat ttactggtgt cgatggtgag attgatgcca ctgtcgatag cgttgttatt
      421 cttgttgggg gacgagggct ggctggagta cttccccctc ctccgagcag agtcatcgtc
      481 tgtgtcgtcg atgaggggga tgttgggcgt ggagtcttct atcctaaact cggggtgtgt
      541 catgaagtta tggatggatg atctggagtc tggtttttcc agtccttcat agagggagct
      601 acggaatgca ttcacaacgc ggatctgtcg taaaaaggga ggaaagagag ggaacatcat
      661 gagaggtgat gccagagacc aaatggaagg gttggaagga ccctcgggat gagaggaaag
      721 agggaagtgg gagagagaag ggacagcggg ggaaggagag aacctctcaa ttgaggggag
      781 gaggaggaga aggtgaagga gcaacaagag aaaaaggcaa ctgcagtctg tgttctgctt
      841 tcacacaaga tgactacagt tcacatgtca ctcaattttg gcgtgcacta agaaaagcag
      901 tgaggaacac aatcataaaa aaaccaaatc atacattttt ttaagaaaaa caaatatcaa
      961 agcaacacaa acgctcgttg taagtatacg ctttagtcca atgtgcggta tacctagaaa
     1021 caagccacgt taataaatgg aatgctcaca gataaaatta aaggagaata tcacatgcaa
     1081 gtttgaagaa atctggtgga cgaggtcagt gagaaggaag gaggttagat ctgagagatc
     1141 caatcaggtg gtttaaaaac ttctggaggt accagagcac tgtctgggaa aatatccaca
     1201 cagactctga tggaacacac ggatatagca cagggtgggt aagcatggtt ccaaacgggg
     1261 caatggagac agtcaaactc aatgcatgag gaagctggtt caacacaagc attgcagcgt
     1321 aaaaacaaca catccaaagg aaccaaaaac agtggcattt agacaacgag ccaacacagc
     1381 cgattaggat tttatgaaca caggaagtaa acagaaacta catgcaacaa gacaacagga
     1441 tggtgaagga gtggacaccg gcacacagac tgcagacaca aaacatgtgg aggtgaataa
     1501 tgaaaataat aagtttactg tcagagccct gacggtcaga gctgttctca actccatgca
     1561 tgtctaagta aacactcaag tataggcacc aaacaaagta cctcagtgag gatagaagta
     1621 tctgttggtc agttaggtca gtttgtggat gataggaaag tgttgcttta atccaaaccc
     1681 tgtttgcatg aacaacatct gctgaaataa aaggatctac atgcataatt ttctagataa
     1741 taataatttg atgcttttac agaacatcga ctcctatcag cacactagag tatattgaaa
     1801 agggaatcgc ttcatcatgg ctgcatcgat aaagttcctg atggtatcaa aaaactactt
     1861 ctgcaggatt attgtgtgta tatggtgact catctttaaa gatttctgtg agctgaagcc
     1921 ataaaaatgt aat
//
LOCUS       KCVB01000330            1407 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15282pri ultra-conserved element locus
            uce-465, sequence.
ACCESSION   KCVB01000330
VERSION     KCVB01000330.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15282pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1407
                     /note="ultra-conserved element locus uce-465"
BASE COUNT          384 a          300 c          286 g          437 t
ORIGIN      
        1 gcttttcccc tttcctaata aagagggttt tttctcagta agaaacatct tattgactaa
       61 attaaggtaa aataacaaat ttattaccca ctaaaggcaa ctgggaatat gtccagttat
      121 agaaatagag atttgaatgt tttatatacg tgagtgtttt taggatggat gggaacctag
      181 catatcagat cagtagcaat gtgtgcaata acaacattgt aagaacttat attaaaatta
      241 aacagctcca aaccatcaga ccagcgtgca gtacgtgcaa gtatccagtc agggtttgcc
      301 ccgaggaaag atgtgctttc tgtgggaacg gggcttgaga agctccgatc ctcatcctcc
      361 caactcacac ttcctgctgt tctgtctgat tgtgtcttct ttgtcttagc ctgatgcgct
      421 tgcctcctcc tctggagagg ctaatgcaga cccttttttt ctcctgcaat ctcagcaaat
      481 gccaagtgta actggtgccc ctattgctcc taattaatgc gtgattaaag agtgaaataa
      541 gggattctac cagtgatttg ttttttcaag gagtaagaaa ttaatcccct ttggatcttg
      601 cagaatcatt taaggattct ccttgttctg tttatcaaat aaaccaagta attagagact
      661 ttagataagt agactaaatt gatgcccgcc ctatgtcttc ccaagctagt gttcgctgat
      721 agaaacagct gtgttgctct ggtaatgtgc agcattatac ttgtccaata acaatgccct
      781 ttgtgtttgg tagctgcata attacacagg ggagcctcct tgaccttggt aagcacatct
      841 atcttacttg tgtatcagtg tatttcattt cttaacccac aaagatacat cacaagggag
      901 tttactgaca acgtaattca gagtacagac tacattactg gcaatgcacg attgtgtggt
      961 atgcaatagt gcagttatcc gggctgatga tacacgcaca gcctctgggt atttaacagc
     1021 gtaatcctca gctaacttat tgtagctcta ctgtatctgg agaagcagca gtgtgatttt
     1081 tattactttc acctgagcac ccaggggagg aatcttatct ccccacttgg ttttatgcag
     1141 tttggttttg aaaggtcttc aaatgttggg aaatttgaat ttaggctgca tgtctgacca
     1201 ggtagacaca ttagctgact tcacttcgct cctttgccat catcccccca cttctcccca
     1261 accatttctg gtgttgcatc agtgtttcca acactggacc tgcagcaagt gtccagtctg
     1321 agacctcaaa cagacacttt gaagccaagt agggccgggc tgtttaaatg acttcatctt
     1381 gtccagatga aagagaaaat gttagat
//
LOCUS       KCVB01000331            1579 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15329pri ultra-conserved element locus
            uce-420, sequence.
ACCESSION   KCVB01000331
VERSION     KCVB01000331.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15329pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1579
                     /note="ultra-conserved element locus uce-420"
BASE COUNT          474 a          357 c          342 g          406 t
ORIGIN      
        1 atcacacatt gtcgcgagtg agcaaacaag gtgagcctcg atggcaggaa aaggcaagaa
       61 ccaacctcag ctgagggctc catgaaacat cggagaagac ataacgcaag agctggcagc
      121 ccttctgcct cgcactgact gtcagagatt aaagaagatg tcaacagcag tttaacacga
      181 gcttaagtga ggaaggcagt ctcagtctga atattctcgc agtatcttag atctgtcaga
      241 aaaccaagca ggtgattctc atccagccca tctgctcgag gaagctcgag gattacctac
      301 ccagagtttc aaatgtgcaa tccctaagaa tagacgaaca caaaacactc cttattaaat
      361 gctgaatgat gctaatttag attcacgcaa ccaataatcc tctcttgtgt taattttgga
      421 gcgcttgaat cgcacaacta tcaagagcat tgttcctctc ttttgatgat gcagactcaa
      481 ttctaatcag actccatggg aagaaaaatg aaatattatt aagtatgatt attctaagca
      541 aggctctgag aaaacgtctg tgttataatc cagggatttg aataatcagg ttgaagataa
      601 tgggctgggc cggcctgggc gtaaatagag agcatccact catcctccca gcgctgaact
      661 taatgaactt ttaattgatg cggttttgct agtccttttg tcatgcaaca gaaacaagcc
      721 tctctgctga tgggcctgtt ttcccgactg gcttcagatt ggtttacagc aggccctaag
      781 taccatgtag ttagtttcat gtgtcatata aaatctgccc aggcacaacg acgcctgcaa
      841 ctaaaacctt aagtgcatcc acaggaatca ctcaccggct ttgcacttcc gtcttaagga
      901 aggaggagaa attcaatcac tgccggcttg acacaccgaa gacgaggcac gtgttcctgc
      961 ttttacttgt ctcaaagaac actctggcaa ggcaggctgg gatttctgtt ggtctgaata
     1021 acatccctga tatgagcagg gcctgagtca ctcaatctgt cagcccgtgt tgtttcccag
     1081 cccacgaact gtgatgcata gatagagata tacccctgtg agccaggtca tctttattca
     1141 atgatttgct tatttaaatg aaatgtgcct gatctgtatc tgacacaaat tcccaataca
     1201 gtgctgcatc agctttgtaa gggggagcat cctcacaaag cacttggccc actttacttc
     1261 ccaacaacca tcagtaacca cagctcaggg actagaacac agcaatctgt taaggagata
     1321 aattatcaga tttggcacaa cactcccatc tctcttgcct ttcgtttgtc tcacaccaga
     1381 aaaaaatgat aattaaatca tttagggtaa acccagcagt tgcacagaga aaatgtaaga
     1441 aagtgtaggg cttagagaga ataggaatag gctaacatta gtaccagtgg taagggaggg
     1501 tgttatgact ggcagtgtca gcacatgatg ccacagatca tgacttgttt atggcccgag
     1561 catcttatca aaagaagtg
//
LOCUS       KCVB01000332            1435 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15370pri ultra-conserved element locus
            uce-799, sequence.
ACCESSION   KCVB01000332
VERSION     KCVB01000332.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15370pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1435
                     /note="ultra-conserved element locus uce-799"
BASE COUNT          392 a          314 c          319 g          410 t
ORIGIN      
        1 tggggtgtaa tcaagtgtta acatcctata caatgttgaa agcagagatg cgtgacccca
       61 gagttatggg agcaagctgg ctggaggggg tgggcttatt tagcaaatat gtagaaatgc
      121 ttttgacctc atagtgaccg cttcataagc ccaccgacac acacaaacct gtcagtcatt
      181 aatcatcatt tgtgatgaga gcaggccttg tttgaacaga ataatgctat gtgggtgcaa
      241 cacgccatca tttttcaaca tggttttcac ttcctacgtg gattgtttac ttggtggttt
      301 ttgtcaactc attaccgaga ctttagagca gcttccatgt tcttggtttc ctgttgctct
      361 ttttgaggct ggttcatggg aggctgtgag aggttgttgt gtcggtggtg gtggagggca
      421 gaagaagccg tgtggatctg gttacccccc gacttttccc acaaaagtga tcagagactg
      481 caggttgaga tcatgtgtac cttgcagcag gtttactcac atttccagtt taacctttta
      541 ttaaactgtc cattgtgctc cctgtctgcc cacagaccct ccgctacatt ctcatgccca
      601 tcaggtcagt gaggttaaca gccaaaggga ggtagcaggg aggaggccaa cactatcttg
      661 tctctgcccc tctgtcgctt gggagcctgc gcaacacatt ggaagaaagg gagagaagta
      721 tatactacaa cggtttgatg agcagcaggg caataaattc attaatcatc attatatgcc
      781 tcatcaaact ctttgccagc ctctccatgg gagttacaca aattagccag tgaaaaaagc
      841 agtaatttta gaggagctag gtaagtgctt tttgattgct ttcttgaaca atgcttcccc
      901 tgacaagcta ataaatcaga tctttctata cttaaagtgc agctactgtg aacccaatct
      961 gagctcccca aacaaaaaaa gggaggccgt gtgggacaag aggttttact gcagccaaag
     1021 ctctttcaca gatttgtttt ggattcctcc tctttattct gcaacaaaga ggcccgggta
     1081 caaagctttt gataactgaa tgaactcctg ttatgtctcc agatgcaacc agggattttc
     1141 tcatattgaa tcaaaataac ctaatgtttg agttgagatg aaaaaaactg agccaatagt
     1201 gaccatatgg tgcgaaattc aattaagtct tctttttcct ttctatttgt taagctagtc
     1261 tgggtttaaa cacattaact caagtgagat gcatagacag acgcatagct gcagcttgca
     1321 agccttagcc tatacagaga atcctcgctg gcaggtgatg catacctact acagcgatca
     1381 tatccttcca tccagatggc cattccatgc aagaccaaca aatcatccat cagac
//
LOCUS       KCVB01000333            1330 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15414pri ultra-conserved element locus
            uce-348, sequence.
ACCESSION   KCVB01000333
VERSION     KCVB01000333.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15414pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1330
                     /note="ultra-conserved element locus uce-348"
BASE COUNT          365 a          275 c          288 g          402 t
ORIGIN      
        1 aatagtaact gttatttgtg ttttttgcag taatgtatat tattttttgc gcctcatgta
       61 ccactgagca gagattttgg tatttatagc cgttattttc tgaataggcg ttactaagga
      121 cactcgtctg tggcctcggc ttacacagtg gcgggtgttt tcagtggcga aacatgtgga
      181 tctatgcagc aaacattaag tgttagtagt ccgcctcatg ggagattccc gggacccgga
      241 tcattgtgaa ttactgtgac cttggttatc aatcattgta tcagggtatc taatgtaaac
      301 gcaaccgtgg cgtggataac atgccacatc tgctctttgg acaacgctgc ttcttggtta
      361 ccaagcgatt tattagagcc ccgtgtgagg aataataggt cgcagtataa agttcaacca
      421 gcagctcttc cataggcgca caattatctc ttaaacaggc tgttgtaaca ttatcattca
      481 cctacattat acagcagata gtttcgtgtc ccaaaagcat ctcaaaaaga aaaaactagt
      541 gcgggttaaa agcagcgtct gcagggaact gtgtttgcgc tcaaggccaa tttattgccg
      601 atgaacatat ggccaatatt ttgtctcacg tcatagcaaa gggaaaacaa acaccatgca
      661 atgtgagcga gtcagcaaga gcagagccac ttggccagct atgcaaacgc tcttggacaa
      721 tatgagaaga ggagcacttg ctttctgggt atataaacca catcaggctt atctctgcac
      781 gcactcctga ctcccatttg gagatatttc attaactctg cgcgcggtct taacgctggc
      841 aacacattca tcccccctcc agtctaggta agcgcactgg atgctccagt cagtttgcat
      901 acagtatttt ctgtttttac agcctaatag tcactgctta ttttcttttg ctgctttttt
      961 atgcgcatca gcgcgcatct taatccttga gcgtgaatga cgctgatctt tttaactctg
     1021 ttcagaatgg gaaacggtta ttcagagtat aggcctgtgt gtgtgtgtgt tttagcccag
     1081 agtaggccta gttggtgatc attacacact cacgtgggca gtctgtgctt ggttgaacat
     1141 tatcgctgtg acggtgcact gtgagagtgg cctgcacctt catgtccgag caaaatcagt
     1201 aaaatgacat tatttggcat gaaaaaagca gatttatgta tgtaaaagca gaattctaaa
     1261 gtattattaa ttttataata tgtttttaga aaaaatcacc agcttctcca aaagcaaata
     1321 ataataataa
//
LOCUS       KCVB01000334            1676 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15458pri ultra-conserved element locus
            uce-1086, sequence.
ACCESSION   KCVB01000334
VERSION     KCVB01000334.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15458pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1676
                     /note="ultra-conserved element locus uce-1086"
BASE COUNT          489 a          377 c          359 g          451 t
ORIGIN      
        1 aacggtgacc aacccactca accacctgga caaaaatggc cctttaacac cctccaccca
       61 caaacacaca catcactcac aagcacctga ctgatgatga tcttcccaat cacgcaaaag
      121 cgaccagtaa tagtattttc agttgcctgg atgcagtttt gaagtgcata aacactcttt
      181 tcatcatttg tacagaagag gatgtgcaca aagaaagaca cagaccacat cacatccctc
      241 cacaacagca gtctgcccct tggagaggac aagaacactt gcaacatctt cttgatcagt
      301 ttctctgcct ttattcctga cagatatccg agttactcta tgacggctgc agtgtgtaaa
      361 caccacctca gaaacagcat tgcaaaacca ccacactaat gacttcatgg agtataaaac
      421 cacaccacaa catgtccaat tgctgttaaa agtactgcca cagcactctc ctgggctgtt
      481 ttttttcata gaaaatgcag ggaatccacc aattttaata acattcagat taaaattaat
      541 tagttcaacc actggcctta aaacatccac attcctgctt cttattggca gtgtgagttt
      601 cacacctaga tacccaatta gcaattaagc ttgcctttga gtgtaggcct ccaatgcggt
      661 gtgtgatcct gctgatttgc atgccgcttg tgcacgcgct gataagcgca ggcagcagcc
      721 atacatagtt ttaaaattca actccagaat ccctcgcaga aggcaaaggg gcctgcaggg
      781 caaaagagag cgaaatcaca tggctgctct tcaaaagggg acctgtgtaa ggcagccggc
      841 aaagtgcgtg ggatcacact cagaatgcag gtaatttgtt ccctgactaa tttgtggaga
      901 tggtggtgtg tgtgtgtgtc tgtgtgagtg agtgtgagag cgactgtgtg cctgtggaga
      961 gtaagttctg aaaacagctt ggtggaattg gatttcctca aagccttgtc atcgcaaagg
     1021 tggattgagt gtaaaatctg taaggcatta tggaaaattg cagaggagag agacgagaga
     1081 gtgaaagaga acgggtggtt gggtgggtgg gaggtggaag ggggtagcgg gttgtgggaa
     1141 agagctctta tatcacaaaa tcactctaaa ctgttggaag caaagataca tacatgtgga
     1201 ccttttctcg gtctgatcac atttgtctct ttctgatttt actgcaagga cagttcaaag
     1261 gtcacctcat cctcacagtc catcagtaag tgtgtgatgc aggactgatc attgatgctc
     1321 atgtgcacaa cagctcttaa aacaccagca cttttcctac ctgttgccat ttagaatttg
     1381 gcaaattgca acaaaagtct cccttgtaag ggttttatag acgcgttgcc aaagtttcca
     1441 ggctttgtaa aacaagtcga aacttttaag tgcttatgga atttgctcat ttacccagct
     1501 tgtgtaattt tgttgcacca cagtatgcac aaacaaatct aaaacaggca cagtgcatca
     1561 aagcctggag tactttggct gctccagtca ctcttgtttt tttccaccac tggtctgaaa
     1621 gtcttttaag ctttttatgc aaaaatcata attttaacat ctttacaaca cacact
//
LOCUS       KCVB01000335            1559 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15479pri ultra-conserved element locus
            uce-764, sequence.
ACCESSION   KCVB01000335
VERSION     KCVB01000335.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15479pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1559
                     /note="ultra-conserved element locus uce-764"
BASE COUNT          462 a          322 c          381 g          394 t
ORIGIN      
        1 attgtggacg ggctgcttgt atagtgtaca gtatgtcaag gcgggagtgt aagaggaagc
       61 cgggggcacc atctccgaga aagaatggta aataaaatgc actcatagga tgcaagcagg
      121 ggagtagaaa agcataagga tgagaggaag aaaaggagga ggtgagtgtc ttcagtgcta
      181 attagccacc tagcacaggc ccttccttga agaataccat gatggtgagg actaacacac
      241 tcacatacac atatgcactg aagcactggg ttgtgttgtg gggggtatac tgtagcatcc
      301 tctgagtgtt aggtaattgg agcgttgtac ctctagagaa aactgctgat ttcataggaa
      361 gtgctaacag gacctttgtg tgccatgttt cccttttcca gggagccagc ggggccagac
      421 agagagagct atgggcctat agtgggaaca gacccagcca tacctgagta gggccagcca
      481 cagcggtaat gaacctcttc aaatggccgt tcacccaaaa ggaaaagagg tgggtaaaga
      541 aaggagggct gagaaagagg agggggcgca gcctaatttc acagccggag aatagcaggt
      601 gggacaaaag gtctagcttg aggagcagag gtgggggggg gctttattag tggtattctc
      661 ctgccccatt tacaccatct ctctcctcgg caatggagca cttatcacaa gtgtacccat
      721 ccagctcacc aatagaaaca agcaaacgct atccgtcaat gtttaccatt aaggatctgt
      781 catttgcaga tccaccatat gtggcagatt aatgagatgc aggcctctct ctctccttct
      841 ctctctctct ctctctctcc agcgtacacc caacacaccg tgtggccaaa ctcaatgttg
      901 ttcggagaaa cggagaatga aaacaagcca cagagaatgg aaacaagtga taaagaggag
      961 tgagatcaag gtgtcagctg gaaggtaaca caggcatatt ttgggcagat ttacacgaga
     1021 gagttaattt tgcactgttc ataaggccac ttaaggtaaa actgtataga actgatttaa
     1081 tgtgagctca gcgtttgtgc ttcctggaaa aatggcggtt ctaaggtggg gcaagagggg
     1141 cccatgcctt cgtaatattt tgcctggacc cccctctggt acccccacat gtgacaaatg
     1201 ttttcctaag gcagggcaaa agtaaaacaa atgtggaacc aatgtattgg ttcatgtttt
     1261 aggtgcaccc atgctatgca cactctgaga aacggcgctg ctgagactaa gttagcatgt
     1321 atgaagttat accaaaaatg tgtgtcatgc tcatgatttt caaacctact caattataca
     1381 aacttataag tacataatat aagtaatgtt tgccttggct aagctgacct aatgtaacat
     1441 ttatccatat tcacttgtag accagcactt ttacattgtc taattccata gtataaccta
     1501 gataccatga tattaatttc tattttttaa ctttttcacc atagatatac tctgataca
//
LOCUS       KCVB01000336            1597 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15552pri ultra-conserved element locus
            uce-759, sequence.
ACCESSION   KCVB01000336
VERSION     KCVB01000336.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15552pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1597
                     /note="ultra-conserved element locus uce-759"
BASE COUNT          493 a          311 c          339 g          454 t
ORIGIN      
        1 aaaagagcat gtttctcact ggaaactgca atcatacatg tcaataaggt gataacttcc
       61 ttttctccaa aaaagtccac ttaacacctt ttattaaacc ccttcaaagt ctttactaca
      121 gataatatga gtctggacag acatgaatgt aaactgcaac ttgaatgttt cacagaggct
      181 ttcaaccacc aggcagtaat tgtagtttct attaaaaaag aggataaatg ccgatcatcc
      241 tcagccctgc attactgtgt agtaaccaga tacaatgtgt gttgttctgt gacagttcgt
      301 gttcgtttca gtgaaaaacg cttcttactt tcacaggtct gacagcccaa agaacaaaga
      361 ggcacataca gatcacatac gtagaggaaa tgtaagtaac agaatatcaa tgagcatttt
      421 ttcttaacgc ctgactttag ccgggtatgt aaaaatgctg ctcctctaca aagcagcttt
      481 aacccagatt attctttggg aattttttga gtccttgaga caaaagagtt ctcttgctcc
      541 aacctccatc tcagcggaga agacagggtg gtacgggaac gagagaatca aaaggctgga
      601 aattcacggc tgagaataaa tgctcgctct tccctgaggt gatttgtctt tgcgctcctc
      661 tttctacaga tgttagaccc cttttttagt aaagacctga ggttctttgg tagcactttg
      721 atatcctgct cggattcaat atgtgaaata gaagagccac tccgtgactt ggagcacatc
      781 acagtctttg aagtctgatc caatttttgc tgctcaccat ttgatgttga aaggatgaaa
      841 tgaggaaaat gtacagtttt tgccagtaac tctgctgagc atatgtaaga ttaaaaaagc
      901 aaaatccttt ggaggatgta cttagaaatg ttaagcagat taatactttc ctatagctct
      961 tttattcaaa gataaaatta aatatgagag gaagtctgta tgagctgttt ttcaccctca
     1021 caattaagtc agtgtggtgc tgtttgctag gaggtagggc tagtaaaggg ggatatgcaa
     1081 ttgcattagc atttgattaa cattgcaaag cacattaatg ggtttgagtt atatgagtgc
     1141 caaattgtaa attgtaaata aatgaataca gcgtctacat gactaaattg aaaaaaaaaa
     1201 gcacattacg aggcttgctt taacttgtgt ctatttatcc gctgctttca ttgacattgc
     1261 tcttctgggt gtgtttttta cgcctcctct ttcaaggttg gcctcgacag ccgagacaga
     1321 caagtgaaag acatcaagag agacaaaggc agagcagaag acagtgtaac gaagccttgc
     1381 agggagacag ccaggctgcg agacatgcag tgtaaacaag gtaagcccga gcacagagct
     1441 ggcgggtgca ggtaattaca caaagagaca gcagaaatcc cgctttaatg gataaagtgc
     1501 catttttaat acggggcccc tttcaacctt tcatcccagc caatcctccg ctgccattct
     1561 ttgttgacag caaaaagaga gaggcattat gggtgat
//
LOCUS       KCVB01000337            1392 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15618pri ultra-conserved element locus
            uce-837, sequence.
ACCESSION   KCVB01000337
VERSION     KCVB01000337.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15618pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1392
                     /note="ultra-conserved element locus uce-837"
BASE COUNT          389 a          333 c          238 g          432 t
ORIGIN      
        1 cgactcggcg tctctgagga gtcttgcctg cctaacagca gtggcacaca agttgtgatt
       61 gcccccactc acaccccaaa accattcact ctctcttttt gttgtctttt ttaaagccag
      121 tgctagcaca ccccggccaa cagtccagcc ctccttgccc tggcagtgct acccctgtga
      181 ggcctgctgg gaacagcggt gagatgttag attagacctt caagtctggg caggacctga
      241 gaggggatgc aacgcaaagg gactgcccac tctgtctatg gctcactctc cattatggtg
      301 taatctgggg gtgtgaaagt atcattccca aatgctgtct cctttaagat cctctcacaa
      361 ttggctgcag aagagaactg gcttgactgg ctgtgttcag tatccctgct gatctatgtt
      421 atcatgtctc attagaccca caatgtttga cagatagttg actatcagat gcttactttc
      481 aaatgtattc atcactgaca agcagctttc atgttcggga taatattgat tttaaaaacg
      541 ttattgttcc tcattgtgca tgtgtgtccc aatctgagaa atgaatgata acctcttcat
      601 cactctactc cctgagtgtt cttactggaa gccatattga ttttgttagt ctgagagtcc
      661 ttgcattgcc tggcctttac ctcagggaca aatctaattg tttatctctg gacagacttc
      721 tgcctttact cccttctgtc tcattatgtg ccaagatttg tcaagtcaag tctctccaca
      781 ctctcaaatc tcgctttaca acatgtcgtt cggcagaaca ctacagatgg cttacttatc
      841 aacagaccct cccacatgga atgttacaaa catgacagca caacacactt gcaaaaatat
      901 acacaaactg acatacaatg taatcacact ccggagacac gattacacat accgacacac
      961 aaaacatgag tcactttttt taagaaacac tgattttttt tatatctttg tgtgtgcttt
     1021 ctcggaaaaa aatgattgtt tgagatctac aatacactgc acaacaatag tattataata
     1081 atggtcttct ttacaacttc attaaggacc atgctgctat tttttcctat tatttctatt
     1141 caatcttttc acagcttagc tcatagcaga ataaaacagt gatcacaatg aaaatctgac
     1201 caaattcaaa tgcaactggt attttaacca gatggttcca gtcaatggac aaattcaatt
     1261 tataatattc tacctttttg caaaattagg agtgtaacgt ttttgtcaga ctaacttcac
     1321 agcaattaca cacaaatgaa tgaccaaggc tactttatac atcactgcca tccctatttt
     1381 gctcatcatt ta
//
LOCUS       KCVB01000338             634 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15656pri ultra-conserved element locus
            uce-872, sequence.
ACCESSION   KCVB01000338
VERSION     KCVB01000338.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 634)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15656pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>634
                     /note="ultra-conserved element locus uce-872"
BASE COUNT          151 a          111 c          149 g          223 t
ORIGIN      
        1 aacagagtat tttttttttt ttttatgtct gtgtgcattg tgtctgtgtg agcgtggcgt
       61 ggtgtaagca caacattagg agagaatgtt tattgtaatg tgagtgtctg gtgccagctg
      121 agctgagctg caagctgttt ggagaagcgg atgactgttt gattgtgtga tgacaatgtg
      181 agccttgcat ctctcttcct ttcgtctgtg tctctgaaga atgctgacat taaatgtttg
      241 atgttggcat gcacaactga tgtacaaata aaaaacctca ccggtgagac atgtgttttt
      301 tttcttctcg cattttgaca atacatttgt tgttcctgtt gtttccacat tcgattcaag
      361 tttacctttt cccccctttt taaaagttta gattgtttta tctctttttt ttttttcctc
      421 ttgaaccaga aagtcgccag aattgaggag caaaaacaga aaattcactc agggtcgcat
      481 attactttag gaataaatgt tttccagcca aacacctctt ggctttcaat aggaggtgtg
      541 tacagcatga cgctatgagg gtggaggtgg tgctggtgga ggagaggcag gtcaatatga
      601 gtcctaataa agcgtgagtt catttccccg ctgc
//
LOCUS       KCVB01000339            1579 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15699pri ultra-conserved element locus
            uce-1302, sequence.
ACCESSION   KCVB01000339
VERSION     KCVB01000339.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15699pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1579
                     /note="ultra-conserved element locus uce-1302"
BASE COUNT          451 a          326 c          325 g          477 t
ORIGIN      
        1 aaacgaaaca aaacatgtgt cagccatgat tttgtatttt ttattttttt ttacccctgt
       61 tccctgctct gacacgtctg cactgagtga gtggcattgg cacactgtca atcgctcact
      121 accgctgcca tcaaacaaca taaaacacac acaggcagag gctggagctc ataaatttca
      181 gcagggcatg atattttttg cgcatcgcct ttatacagcg ctatatcctc attaatttct
      241 tctcgtggtt gctttttttc taaacagaaa atatcccaac tttttgtaca tttcatattt
      301 ttttatagtc acatttggaa accttgtgcc gtagattgtg tatttttttt aatactgtca
      361 gttgtcgcgg gaatcatttc tgcacattta tagttggcaa caccttcaag attttttatt
      421 tgtttacacg tttctacatt ggatattttt gttgccggtg cttcacgagc aggatttgga
      481 catttttttg gagaggaaat gcgccgggaa tcccacgacc agcgctaata aatgagatca
      541 ccaaggagcg tttctgtctc taccacccgt caaatcccag agaaaagaca gattggcatg
      601 aggattcgtg cgggatacga tggaccgtca attttgacaa agaagtgtta aaaagaagaa
      661 aaaaagattt atgcattttt ctacaaacat agtcacgcgt tttggcactt tggaccgcgc
      721 gtaaaaaagc gagaggacag cgagaacttg gacacaagat ttacgctaaa aacatcacgg
      781 ctgtaggtat tttccttttt taaagaacgt gtgtgctccg tatcggtgaa gaatcacaca
      841 gccgtgcatc accccccttg tctgcggcac atgttgtagc cgatttcgta tatgagtttt
      901 tcaatttgtg tggctcagtg cacagactct ttagcggcta ctatggacga tcaagcccgg
      961 atcatgcagt ccatcggcgg tgtcagtctg gctggccact cggtacaagg aggcatggca
     1021 ctgccgcctc cacatggaca cgatgggaca gacggagagg gaagaaaaca agacattggt
     1081 gacattctcc atcaaataat gaccataact gatcaaagtc tggacgaggc gcaagcaaag
     1141 ttggttgatt tatatgtttt attaacttcg cgcccccaaa acgctgaacg catcattact
     1201 gagatgtggc ccgcttaact cccccatgta taacaatagg ctggagtggc tttaatgata
     1261 atcttacact aaggtgtatt tacagcctcg caaagcttta acacatgaga aagttttcac
     1321 tgagggaaca acagcttttt atttaaactg cctgtgtgcc aacagtcagc ctcaatgagc
     1381 ttgttcaaaa aagtttgtgt gcacacatgt cttatagtga actttcatat atctgtaaaa
     1441 ctaactggta gtgaaaaaat gaaattcatg tcagtaggtt aaacatataa attagttgtc
     1501 actgtacgtt atatttataa tggagcattg tattactcta aagccaatac tttggttttt
     1561 attttattag agcaaatac
//
LOCUS       KCVB01000340            1977 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15744pri ultra-conserved element locus
            uce-458, sequence.
ACCESSION   KCVB01000340
VERSION     KCVB01000340.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15744pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1977
                     /note="ultra-conserved element locus uce-458"
BASE COUNT          464 a          477 c          416 g          620 t
ORIGIN      
        1 gaactttaca attaatattt tgggttcaga acttgttttc ttgcagtcac ctattggtca
       61 aaaaatcagt ttatttcatt ttttattgac aaatcaatat agtatattta ggctatatta
      121 tagtttttaa ggtgaaaaaa aaacgtgtct tttactacct cctgattgct tctgttacat
      181 aaataagctt aatatcaaaa gttgcgttga gaggaaacac atctttgtca cctcattaga
      241 gcaattttaa ctgttagctt gtcaatatgg cttcctactt tgcaaccccc tttctctttt
      301 tcttttcctt ctgttttcct aatctcagtt ttgctcaatg ccctttcttg ctgttgtgtt
      361 ctccttgtct gcctcttttt ttctcccttc tgaatggtac taatcgtcta gctcgttgaa
      421 ggtagaatgg cacaaggcct cctccttcct tcctttcctc cctccacccc tcctcgctct
      481 ccactcctgc actgtcagta atttttcatc gcccttgttg gggccgtttt atgcattgtt
      541 tccctgatgc agtgattgat gtcaggggct ggtaatctgg gagaatgaga ggctggatgg
      601 tggccgccga gtcccctctg tctctgacac gtgtcggctg agcggcggtg cgctgtgccg
      661 cgctggggcc cccgcttggc cgcggccact gctgaggctg ctcacttcgc tcactgagcc
      721 agccctcatt tccatttcaa tctcaacctc atgaatgcaa aaactctggc cgaaaattaa
      781 taacaccaca caggggaaaa gtgggcgcaa aggagaagga tggaaagaca gagagagatg
      841 gagaggagag tgagaaggag aatgtggagc tggggtcttt ttcacccctt ttgttccttc
      901 aaaagtgccc cttattttat tcccctctct ctttcaccag gatcctccaa tgaatggcat
      961 tacagacaat gccatatcca gagtgacaca aatttatttc ggttgcagcg ccatagtgta
     1021 tttccacaga accaggggcg atttgtctgg ggacaatttg gtttccataa aggatttaaa
     1081 tggtttctgt ggagatagtg gggtgaaatt taatttgttt ctcatttact acagcaggta
     1141 tgaaaaggag aaaaattgga cggctgatgg gctaaaaatt ggtgctgtcc ctctgctgta
     1201 tccccaaagg accaaggccc tgcatcatat agattgagtt cgaccccccc agccctccca
     1261 accacaagcc aagccaacac gcacgcacaa acacacacac acacccagcc tgcctccagc
     1321 ctggagagag ctgtaacctt aaaccaactt aaacagtaac ctttggggag gctgccaatc
     1381 actcctaaaa caacgtttcc atcgatcggg ctgtctgcgt gatttatggc ctcattttac
     1441 actaattgag aattagaggg cggattagtg ggcctcaaca cttcggcctg atatgcactc
     1501 tgagcagtgt aatctgtcac accgggatgt ccgacactct cttactcaca aaggaggaga
     1561 ggagggagga gagcgggggg ggggggtcct ttctaattga atttccagat aacacggaaa
     1621 ttaatttgac accagtgaaa tttgactaca gctctggtgc tgtgctctct tgctccccca
     1681 ttgtgaaatg tatgtgtttc ttctctttct gtctctcttt ctttcccttc atttctctct
     1741 ccctggctct ttctctcttt gtttctgctg tgcctagcaa acactattga tccaaatcac
     1801 tttgagtaga gggccgtctg aagcaaggaa tctctaaatc atatttgtgc tcatattaag
     1861 ccagttcatc tgctgctatc ttcggccaga taaagaatat tacctttcat tttggttgac
     1921 tgtgttcaga ttatcttttt tttttctttc tttttgtgga ggggaatctc ctttccc
//
LOCUS       KCVB01000341            1760 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15785pri ultra-conserved element locus
            uce-375, sequence.
ACCESSION   KCVB01000341
VERSION     KCVB01000341.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15785pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1760
                     /note="ultra-conserved element locus uce-375"
BASE COUNT          612 a          268 c          294 g          586 t
ORIGIN      
        1 tataaaaagt atagataaat aagttaaaac ttgtgcattt ttcgttgatc acatggttta
       61 tgtttcctga gctagtttta gaattaaagg ttaacctttt ctctctcaca ctgtgtgtgc
      121 tccttcctgg aggagaaact gtctcgcagc acagcactaa aaagaaaaaa aaaagaaaaa
      181 caataagaag atggaacatc tcaaaaaggc tccaaaccac agagggcaat caggtcctgt
      241 cccccataag aacagggcat gatgggaaaa ggctgctaaa tgacaccgga tctctccaga
      301 agattcagtt tggacatttg tcatgcccct ctttttttgg ttcatgaatg aatgttgccc
      361 tgtaggtttt aaaggaaaaa aaaagttctt tgtttgaatt gcccaagcac tggattgtgt
      421 tggttttata catttttatt ttaatattat tttatttttt tgtaccctgg actctcattg
      481 gtaagagccg attgaaggaa gcttaatggt ggaaaagcaa catcctacgg cgcttcttta
      541 tctgcggatc aacattcttg ccataaggac aatactgaca caaacgttga ttctgtggca
      601 ctggagaagc atttgatggt tgatgcacca aaaaacctaa aacttaaaaa aaaaaaaaag
      661 aaaaaaaaac ttaaattatg aactcaatgc ttttgggggg gaaaaagctg agagaatatt
      721 gtaacaaact tcgtacagag ttcagtctat taattgtttc atgttagata ttctatgtgt
      781 ttacctcaat tgaaaaagaa tgtttttgct agtttcagat ctgctgtggc attgatattg
      841 tatgtccatg aattccttcc tttttcagca cgtgttcctc actagatgat aagatgccgt
      901 ctcccttaag ctttgtcaaa aatacattaa atacttgtat gaggactgtg acgtaatgtt
      961 taaaaggtgt tcagtcacaa atgctgtaat aaatatttca tttttgattt ttgttagatt
     1021 tttgtgtgat taactgtgtt acaacagagt ctttatccac attcccatat tattagcatt
     1081 attattatta taattattat tattagtagt agtattagta ttagtattag tattattttt
     1141 attgagaaat aacatgtttt gggtatactg ttgcctgtca acaaggtctt aatttttaaa
     1201 tttattttgc aaatggtcaa aaaaaaaaaa aatcaacagt caagttccca cttttttcag
     1261 cactttattt gcagtggttt gtttttaact agcctgccta ttttaatatt taatatcaat
     1321 attgcaaaaa aagaaaaaaa tagttgaaat aaatcttgcc aatgcagttt atttaggcac
     1381 acaaaaatgt gccctcaaaa ttctaaaatt actgtcagat gcaaataaaa ggtgcaaatt
     1441 acacagtaaa tttgccacca actcatcgta gttttggact ttaaattaac tgtcagcagt
     1501 gatgtgacgg taaataaaaa aaggccaacg tggcctccag tgctgacatg taaggacgac
     1561 aatgacagaa taaacaaaat aaaccaaata catgaagaac atttaattta tacttaactg
     1621 tctagaaaaa aaatcccaat ttgctcttca caaaaataaa tactaattaa ctaaatgtag
     1681 attaaataat attttagata tttctgagta tccagaaagg ttttaatttg taaataaaaa
     1741 agttaattta gtttatggat
//
LOCUS       KCVB01000342             984 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15818pri ultra-conserved element locus
            uce-330, sequence.
ACCESSION   KCVB01000342
VERSION     KCVB01000342.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15818pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>984
                     /note="ultra-conserved element locus uce-330"
BASE COUNT          253 a          194 c          187 g          350 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgctgccc tccttttttt taaacatttc agcagttttc
       61 tctcctcttg ccataaggtg tttctatgac tacgttatgt aggcctgtgt gagcgggtca
      121 gaggcggggg tccctgggag aaaagggccg catttggctg atcatttatc aatgtcaacc
      181 gcataatgat tctccctgca gtccccctat tgatgaggat aattacatta gctcagagtg
      241 actgatcaat aaagcccagg gaaaatggtt tattatagta cgaccgaaaa ggctttcttt
      301 aggaggggac tggctgcctc ttcttcttct tcttctattt tttttaataa aaggcagggt
      361 tgagctgcac aagcaaactc cggaaagata atcgagtgag aggcaaaact tcatctggac
      421 gcagattgtt gtgattttga ctctgaattt tttttttttt ttcaggactg tgtttctagt
      481 ttctcatttt ctttctcttt tgctttttat tgcttcaagt taatcttact ttattttttc
      541 ttcaaataat tcatcttctc aggatagaaa aagtacggct ttacacccat ctactttaat
      601 agatttaaag ccacataaat gcacatcttg tttttttttt ctttcaagtc aaactctgct
      661 gctaaccact tcacagctaa gataagctgt aatttaatat actaatatta ttttctaaat
      721 tgtttttgtt gcatcttgtg ggaaaattcg acgttcaaat ggcagcaaca cctgatgcag
      781 tgtgataaaa tcgagccggc cttgaaggga acaccttgaa atgaagccta aacaaaaata
      841 agtcttgttt tgttggtcaa ctagcaaaaa tactacgcag aaataaacaa aaatatcctc
      901 aaaagacggt cagtaaatac atttctgtcc tctctcttcc tctctctccc tctctctccg
      961 cgcgtgtgtg tgtgtgtgtt cttt
//
LOCUS       KCVB01000343            1533 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15921pri ultra-conserved element locus
            uce-601, sequence.
ACCESSION   KCVB01000343
VERSION     KCVB01000343.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15921pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1533
                     /note="ultra-conserved element locus uce-601"
BASE COUNT          481 a          260 c          264 g          528 t
ORIGIN      
        1 cccacagtcc aaacacttgg gagtgaagtg gactgcactc ttgaatagcc tgtacagtag
       61 gtgtgaaggg aggtgtgact gtgattgttt attaatcact gttagatttg taatggactg
      121 gcagcttgtc cggggtgttc cctgtcttcc gcccagtgca ttctgggata caatccagcc
      181 tgttgttcag gatatcgtca cgtccaactg tatttggaat caacacaggc taatattatt
      241 tatgggcttc atttcagcta atacaggtca tttatctgat tatatttact ccaagaatac
      301 catctgtcta tctatctatc tatctatcca tctatctatc tatctatctg gttttatgca
      361 ttcactggaa gtcagtttgt agtttttggc agtgcattcg tgtgagaggt catgagtgga
      421 atctttattg cataaacttt acccttttaa agataaaatg tgaccttttt aatctatatt
      481 tctggccagt atgttgatga gaaaatccac gaaaacccac cgagagaaag cagagaaaaa
      541 taaaacggca acaagacttt ctgtgaagta caggaaagta ctagttttta ttccttcata
      601 atcaaacttc acaaacagct tgaacttgta caatacctca gagagtgaaa attacaaaaa
      661 aagtgctggt aacatttaca aaaatcatcc ttacttagca acaatcagtt tattcttcca
      721 cacttttttt agtcttttgt attaaggctt aatacttctg tataaagtat atgcaagata
      781 agtcttcaca gtttttcgaa aaaagtcaca atattatgta acataatgaa gcttttacgg
      841 ctattttctt tataataaca aatacacatc actggtaaat atatgtgaaa tacagggctt
      901 attgtaaaat ggcaagtaat gatactgttt gctgagataa attgattcca cacactgtta
      961 taggaaagtt ttgacatacc attgctataa ctgaaggagg aggaaaaaga aaatgataag
     1021 cataaacact tgggagatcg cctccaaact ttggcatgtc ccatgaaaca attttcacaa
     1081 aatatgagat ttatttttga aatcatacat atggaagaat agctaaactg gcataaagtg
     1141 aaaacatgct gtgtagtaag gtgctaaatc agagatatta catagccagc cttttttttc
     1201 cccacatggg ctgacatgaa taaggtgcat ttgtaagact caaagcatgt aggcctttct
     1261 tagatgtcaa caaaatatgt aataaataca agttttcaaa aaacaaccta aattaatttg
     1321 accaatagtc ttgaggcgac ctcgtatact catgcattcc catggattca gagaaatgaa
     1381 tatctctact gcagttgtgt aactctctaa ctgtggcatg tgaaagtaaa agctgtattg
     1441 tattcatggc atgtgttatt tgcttttttt ttttcctatt tttatttttc attttatctt
     1501 tttttttttt tttttttttt tttctatttt ttt
//
LOCUS       KCVB01000344            1507 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 15961pri ultra-conserved element locus
            uce-295, sequence.
ACCESSION   KCVB01000344
VERSION     KCVB01000344.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="15961pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1507
                     /note="ultra-conserved element locus uce-295"
BASE COUNT          416 a          360 c          335 g          396 t
ORIGIN      
        1 gcctgacgca caaacacata gtgatggaga gagagagaag ttcttggtgt aaacaaagca
       61 agaatcacaa aatgtctctt gtagaaattg tacctgaact gaaatgcctg tgattttcga
      121 tttgtttgtc ctttgcgtta tttttggttt ccaagggaga cataaacgga caaaacggca
      181 agtgctccat tcattgaatg gctgtgcgtc atcgcagcag atccaatagc aagcgcgcat
      241 cccgtcgtgc caaatataaa agcgactctc ctccgacttc cagagactaa atccgtttta
      301 atccggttga tgaacaagta aagaacaaca tatgtctact gtgtttaaac ggtttaaact
      361 ccatatttcc atgtttttac acagagacgc ataatggatt taaacttaac gctctcacag
      421 cgcgacttgt ccgccttgag cgcgaaggcg cacagagtgt ttgggaatca ctcactgttt
      481 ctatttgata gcaacagcac cgcttctaaa acggaggagg aagacctgga agtcaacacg
      541 gacgtttact ccagggtgat agtcactgtc atctacgtgg ctctgtttgc tgtgggctct
      601 ctggggaact ccatcaccct ttacaccctg ttcaccaaaa agaccctgca gaacctccag
      661 agcaccgtgc attatcacct ggccagcctc gccgtctccg acctgttgat ccttgtgctc
      721 tccatgccca ttgagctcta caacttcatc tggttccacc acccgtgggt gttcggggac
      781 gccgtgtgca agggttacta cttcctccgg gacagctgct cctacgctac cgcgctcaac
      841 atcaccagcc tgagtgtgga gcgctacatg gccatatgcc acccgttcaa agccaagagc
      901 atcatgtccc gcagccgcac caagaagctc atcagcgcca tgtggttcgc gtccttcgcc
      961 ctcgccacgc cgatgctctt catcatgggc caagtgatgc gtaaaggcga gaagatatgc
     1021 accgatgtcg tctcccctgt cactattaaa gctgtgctgc aggtaggtgt cggagaagtg
     1081 ggctgcttgt tagttttaag ggaaaatgtg tccagaattc cccgtagtgg gtgtcttgtt
     1141 tatttagatc agaaatagaa agaaaacaat tgccgacgca gagaggatgc agaatgttga
     1201 gctaatttgt ctttaattca ttagactgta ttaaaataaa tttagccata gggttgttag
     1261 taacttcact ttccaatttt aatttctgcc aggaaatgaa caggacagac tcttactggc
     1321 atcaacaaca tggtgcatgt ttctgtgaaa ttaatgaaac atgatgctga acgcacggta
     1381 aagatcagat cctcagttct ttatttctga aaaaacggat ttgttaaccc gcattatcac
     1441 tgaaatctaa agcagaaaga tcatgaaaag cttttaaaaa gatgagcaaa cttataacag
     1501 cacgatg
//
LOCUS       KCVB01000345            1208 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16001pri ultra-conserved element locus
            uce-644, sequence.
ACCESSION   KCVB01000345
VERSION     KCVB01000345.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16001pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1208
                     /note="ultra-conserved element locus uce-644"
BASE COUNT          344 a          206 c          234 g          424 t
ORIGIN      
        1 gtagaaacaa agctcactga tttatttatt tatttttttt tttactttga agtaggaaaa
       61 tgtaatactt ttgatttgct gagaatacta cataccatat agggagaatt cagccgctgt
      121 gtgtctacat gttcctacat ctatcatggg ggatttttct cctgctcaca tgtaaacaca
      181 cagacatgct ttcagctagc gtcaaaggtg tatttgcaaa accaagccat ttacttgact
      241 cataactcat aactcaaatg ccatggatgt gttgtccagc ttactgtgcc tgatgatgcc
      301 atgcagtgtg tgtttggggg ttgttatagc accggggagc cttacgtaac taactaactg
      361 gagggtaggg tttatcgaat gaattgcagc tgacttccat acctcacaca gcgttggtgt
      421 tgtgagcgag accacatgag aaaagggcaa attaaaaatc atggatactc tctgactgtc
      481 aaactgtgca ggtactcacc ccaggtactc ctttctctac ccacatccat ttctgaatgc
      541 tgttgcctgt cagaaaacaa ttctgtactt cttcagaata tgaacttgaa agcttgactg
      601 tgtatatttt tattgtgttt ttttttttgt tcttttctat ttattaactt ccaagtatgt
      661 ttggggataa aagtcattta atagaaaatg tcagagacac acattttttg agcgctgaga
      721 tgcacaaaca gcctgcttga tatacctacg ccatcagcta acagtgtgta tttcagcgta
      781 gtgtttttaa taggatactt ttaatgtcac actttctcag ttggtaggat gccgtgttcg
      841 tctctatcct tattggtcat cgaatcagat aaaaatatgt caagtgcaat tttgcattgg
      901 tgtaaggaat atcaaacagg tttgtcacag tttttttttt tttttttttt ttttaagtta
      961 gggtttaagg tagtgaccaa accatgtaga cttaagtttg ataataaatg caaactttat
     1021 cgaagcaata aaaaaaaatt taacagaaaa atatgtacga aatatcaaga tattccggcc
     1081 aaattatagt attgtgcaag tatatggcta gtatttgtgc attattagcc gtgtgtcttg
     1141 tttatgatct gcactttcct ttgtggttaa attgagggac aaatgagact gatagtcttt
     1201 ttgtagac
//
LOCUS       KCVB01000346            1675 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16034pri ultra-conserved element locus
            uce-351, sequence.
ACCESSION   KCVB01000346
VERSION     KCVB01000346.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16034pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1675
                     /note="ultra-conserved element locus uce-351"
BASE COUNT          502 a          309 c          325 g          539 t
ORIGIN      
        1 atattaaatg ctatttgaga accatcaaaa cacgcaaaat tgcaaattgc ccagcttgta
       61 tctgaattaa tacctcattc atcatcatta tgagttgata agttaattta accatttcat
      121 tctgtcttaa tgagctatag ttgaattaat gtcatgtaat atatgaaagt aacatttgaa
      181 cagaggcaat gatttgagac aaacagagca gagactactt tagaagcata atggtggcat
      241 aaatctcgtc aggctgcagt gcatgttgct cattttcctc acattttgga aaaaaaaata
      301 ttccggctga atagataaga aaattcccaa cctgaaaatg atttttaggt gtgaagtgct
      361 ggcaagcttt ttggcagtgt ttgatgggat ttgattgatt tgaatatgtt gatgtaacag
      421 ctggtatgtc agttgtaaat acggtgctgc accacattaa aatgtgagga cattatgaag
      481 aacaaggctg agctatgttg gagtgtcatc tcacagttgt gggtatattt ccctgctttt
      541 aaactatcaa taggagcaac aacaactaca tgtacaataa tgttatattt ttagtcctta
      601 cttggctcac tgaaggccct actgagctgc cagtccttca tctggcgatg agtgactgca
      661 tttgaccaat aggggtctgt gctgtcttct agttccacct taaatgcgtt ttggccccgc
      721 ttgctccctg tagtttaggt tttttgtcct cccgcagcag ctgtcaccct gcattactcg
      781 cagtcagcta aatgaaacat tattctaaac atatgcatcg taatcagtcc ggtcacactt
      841 acaagaacac gcgttaataa ggcaatcaat cactctggaa caagcaagtt gttctgtaac
      901 agctcataaa cagtgtgtaa tgaagaattg gaggttagca tgacacggcg ctgatcagat
      961 aatcaatgtc aaagatgcga tgaatgtcag taaatgtagt tttcatgtcg tttctataaa
     1021 atcctcaatt tacaacaagc agttcaatta ccctgaaaat acagtcaatt aaataggggt
     1081 gattggacag taggggcagg atcatcgatc tttgcatttc tatctcgctg gtggacattt
     1141 aatttggttt ttctattagc accgaaataa agaaaatata aaatatatta aacaacccaa
     1201 agatttattt tcttgtcaaa aaccattcag caaaggtgac agacagtctt ctgcattatg
     1261 atgaattctt ttttcagtct tgcacattgg atacaaaact aatcgtgtta ttgtgggcag
     1321 tgtttttctg gcctctgtct tttcgctttt acacctccat ctatctcaat gcttttgttg
     1381 tagggttgca accctcgctt tagtaaagag caaggaaaca tggtgatata tcacgcccat
     1441 atttgctgcc ctaatcctat ttctttaatg gggacgttca aaaaagcaac ttatcttaaa
     1501 gtcccttggg ggcattctgg tgtaggctat attttaacca agtgcaatta acgacagtat
     1561 cagcttgtat catttagagg taatgtaaaa ataatggtaa attatagggc cggaatgacc
     1621 cgtgatggca ggcctcatat tccctgtagc tttccatgtc tttttaatta atatt
//
LOCUS       KCVB01000347            1151 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16078pri ultra-conserved element locus
            uce-314, sequence.
ACCESSION   KCVB01000347
VERSION     KCVB01000347.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16078pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1151
                     /note="ultra-conserved element locus uce-314"
BASE COUNT          422 a          215 c          239 g          275 t
ORIGIN      
        1 tggtgaactt gaacacacct cgcaaaatgt ctgacaagct gtctctagct tctgaggctt
       61 tgaaaaaaaa aaagaaagcc acagaagtat ataaaaaaac tagatcaatg gaactagctg
      121 taagagtaca aaaacaaata aactgcttca taagtgtcat gcaaattgca agaaaaaaac
      181 atacaaaact atatcctcca aatgccaaac tttcataaat ctttggaata aactaaggga
      241 atcaaaaatt aaacaacaca tgtggaaatg ttgtttctat tcttttcatg taggggagta
      301 catctgttaa tgccctgaat ttataaatgc tacaatgcaa gccacagcat ctggcatatg
      361 tggttattgt ttgattgttt tctactttct ctcagtctct ctatgtctct ctttcccttt
      421 tctcttttca tccctccatc gggagcccgt agaagcaaag gattatggat ctctggaaag
      481 cgttcaccct tgggtcagaa gacaagacaa ggcgaggtaa tgaaagataa acagctcgct
      541 gcaaacacct gttaacgtcc gcttcaaaga aaagacaggg aggagggggg gtttgcaggg
      601 aggggaaaac aaaaaaagaa gtatttggct gccaagcaac agccgaaggg ccccccgggc
      661 tgttgacaga tctttatgaa atgaaaaaaa gagaaaagaa acgacaatga ttgtgggctt
      721 tttacaacag cgatggatga taagaggaac acaagagaaa acacaagcaa tcataaatat
      781 acatctttac tgagtcctca ggcgaacctt ttgtgacaag cacacacaca aaaagacaca
      841 cacacagaca cacagtcgga aaaggctagg cactcaggta cgcacacaac agcgaataaa
      901 acaaggcatc caaagtgtta aatatacaca ttcacttaac taataaagcc caaacaggta
      961 aaaagctgtc ggattaattt cttaagtgtg taatgaatgt taatgacttg aaggaaaagc
     1021 tttctaagga cagccagtgt gacgcaaatc aaactttcat aagggttaag gggtgttgaa
     1081 tacttaggga ctaaggaggt atagaggaaa cagagaaggg actgaatgta tctgtttaag
     1141 ctattagtcc t
//
LOCUS       KCVB01000348            1479 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16137pri ultra-conserved element locus
            uce-54, sequence.
ACCESSION   KCVB01000348
VERSION     KCVB01000348.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16137pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1479
                     /note="ultra-conserved element locus uce-54"
BASE COUNT          480 a          261 c          247 g          491 t
ORIGIN      
        1 tactcatgag aaaaaacagt gtcaatgcac aaacaagttg tttgtgatgc aatacatact
       61 tcaagttatt ccttatattg catttctaag cttttgcttt ttattattga ttagatattt
      121 tatcacattc taaagatgtt aattttcatt ttgtttagat tgggagtcag caataaagtc
      181 aacaaataga aacttcactt tatggtttac atgatagtga ttcttttatt aaatttttct
      241 ataaaatgtt gcaaaaattc aggcagtaat gctaaaaatt tgcaatgaaa tattgcaata
      301 aattgttagt aatgctggaa aggacatcag tcatattcag tgtaaaatca gacctaatgg
      361 atgcataaca ttgtccttca actgtttagt cacacatatg atttaatgac attttaagta
      421 aaaaaaaaaa tatagtaagc ttgcatcata accaataaaa aggtttaaaa acttctgtcc
      481 caaacttcac cttatcccca ccagtttaaa aagtgttttt ctgctacatg aagcgcaacg
      541 atgctcgaaa atatgattct tcataaagag cgtgaatatt tgcgatgtgg ccattggtaa
      601 aaatccttta ggggatttcg atatctaaaa gagagaggtt tatacattag cgctgtagtt
      661 gcaaaacact gttgatattc aaatgattat tgtgcaaaat ctgggataag cttctgtgtg
      721 agagctcttc taagtgccta caactattgc taattcccct cgccattgtg ggccgaacaa
      781 aggccttaat cccatttcac ttttgcagaa ggctggggct attttccctc tcataaacca
      841 acaacactcc ccctagccag agctgagagg ttctgagaaa ggggccagtg tgtgtatact
      901 tcaaggcttt tttgtccaac ttggctagtg ctctgcataa acttttaagt ggtcccagta
      961 ggcctcaaac accttttggc agaaaaaata agctaccaca aactgtaaaa aggaaaccaa
     1021 acactctctt aacctggaca atacaaaaga aaatatatga agtcttttat aactcggatt
     1081 gggtctggac tgcaaagagt taacatgttt ttttcatgca ctagtaaagg aactttcatt
     1141 cttctgaaag cagctctctt tctctgcatt gtaaatcctt taatacatta tttataacat
     1201 ggtggtccac tatcaaagca agtgaaggag gacaggattc agttcttgta ctttgtagtt
     1261 tgcgttagct tgcagcggaa aataaaacca cttctctgac tttgacataa tattttcctt
     1321 tgttattcaa cccacttcat ttctgccttt caaatttatg gtttaattaa aacaagtaac
     1381 agggattttg tccattcaag tataattaca tcacagagtt ctcagtgcaa atgtgtgtat
     1441 tgttactttt actttttaat cgttaaaaaa aacaggtat
//
LOCUS       KCVB01000349            1572 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16219pri ultra-conserved element locus
            uce-856, sequence.
ACCESSION   KCVB01000349
VERSION     KCVB01000349.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16219pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1572
                     /note="ultra-conserved element locus uce-856"
BASE COUNT          468 a          327 c          358 g          419 t
ORIGIN      
        1 aaaaaaaaaa aaacacaaag cccataaaac aatggtcaac tgtttttgac gttagcagac
       61 tattaattgg agagccgggg tatgctgtct gcaagttaaa gagatatggc caattaaacg
      121 cgctggagac gcgcggggaa attaagtcct atgaaatcaa agcgaggact cattttctgt
      181 aagcgtgtgt gggggggtta gagtgtttat attattcttt aaatgcaatc aagggcttaa
      241 atagagctat gactaaatgt ctgaaagcta aaagcagctt gtgtagtatt tccactgagc
      301 gcatcttgtg tgcgtttaag tgatttgtgt ttttgcgggc agagttggat ttgacacttg
      361 ccgcccggtt attgtgacag aaccactgag gggatttggt aagcagagga gacgaacggc
      421 agcatgccag aggaagagga ggaggaggag aagggggaag aggaggagga ggaagaggag
      481 aaggagcagg aggggggctg ctttccttac tgtcagcttc tttcttgatg atgtctcggg
      541 gtttttagaa actgcgtctc tgactcgacg gcgccactca gccgccccct ctcgactgcg
      601 cagaatgagg cactttggga aacagtaacc ctttcaggct ctgcccggag agtattttag
      661 aagcgtgtga aaggcaatac gccgccctgg ctccagccag ttaggtgcgc accttttaga
      721 aacagaccct gccagccagt cagatcagtg gttctcaaag tggggtccgg ggacctccag
      781 gtggtcctta aggggattct atggggtcct tagcaaaatg atgaatagtt aattttcaga
      841 aaagttctta attcacaaga atgaccccat tttgactgtg tatactcatt ataactttaa
      901 tcttgtgcca ctgtaatcat tatcatcacc tgctaaataa tcaatcctct ttaaaacaaa
      961 ttaaaatgat agatatgtat aatcaggaca catatcttaa aagatatatt ctggagttgc
     1021 tattttagat ctcagcacta tcctgaaagt gttgtctgat tagataagct cacatttctt
     1081 ctaaccctgg ctgttttgct ccaaatccag ccccggcttc aaatccactg cacatccact
     1141 aaattaatcc catcctagaa atagaaacct tttgaataca cagttaatat agaagcatct
     1201 aacacggtcg caggttaaat acggaccgtg tggtttttct tcttacaccc agggaaaaat
     1261 gccaatcctc aaatgagtaa ctgtcagaga ctcctgtctt cacgttcatg ttggctatat
     1321 cacagcttca caccattcat aaaatctaaa ccttcattca tcagcaaatc gcaaaatgtc
     1381 atcataaaat ttgacttgat gcaaatgtac agtcctcatg gctacagaca gacctttgat
     1441 tgacacgtta cgctgagaaa aattcgaaaa tgtctcccca tcaacacagt cagagcgcca
     1501 catgtgaagc gttgacacat cagagcggcc tgcattcaca tactgttcat aaaaatgtca
     1561 agacagtggt gg
//
LOCUS       KCVB01000350            1632 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16308pri ultra-conserved element locus
            uce-1027, sequence.
ACCESSION   KCVB01000350
VERSION     KCVB01000350.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16308pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1632
                     /note="ultra-conserved element locus uce-1027"
BASE COUNT          431 a          376 c          403 g          422 t
ORIGIN      
        1 actgttaata tcctacaggc caaactcaga gcatttactc ctccagtttg gttcagacta
       61 atgcaaagtg taatttgggt atattatcta agcttttact ggtaggagag agaggaggaa
      121 ggacactggg aaacaatagc aaaagagaaa aaaaataaca ctgatgcaga cctttcacct
      181 gaggtcgaaa cacaggtcct ctgctgctaa tctgacatat gttttcatga gccaagacaa
      241 gcaaccctgc catggtggcc gctctccaaa actaatgatg tcaagtgatt ccctctcaca
      301 gaatcccttt taaaacagaa tgaaagaggc agagagggag ggaagtgcgg gggcctgtgg
      361 gggtccagcg aaggggtgca gttgaagggg ggcggggggc aaagaagaag actgagacga
      421 cccccgcatt gtttaaaaac ttaacttggt gcagctcaga gaccctaccg gctacctcct
      481 gtgccaaaac ttacccctcg ccactatcat atcattgtac aaagagaaaa gggctcactt
      541 tttatttacg ccgctgttat tgccagaata ttgcttttaa ttgctgtcct ggattcccca
      601 cggccgtata ggatgagtct gagacgatta ctcaaagtaa tggagcataa tgatgtctcg
      661 gaatggcccc tgaagctcag gtcaggggtt ggacaatgtg aacattcagc tacttagctt
      721 cctttgcaac tttgtgacaa aatctgtttg cccaacacca attaagatgc ctctctgtac
      781 gctctggtca atgggtttga cggagggggt cgtccaacga gttgacatgt ccctgagcac
      841 gaacaagctg gcctgtgtcc tcgcctcaat aattggtgtc ccttgcaacg tgccgcttcc
      901 cagggctgtc ctttgtgtcc ccatggcagt cctaaatgag gggagtcagg gctttaatgg
      961 gactcctgtt agttttaaac aaacacaggg cgacccccta ctgttcagca tgttgcattg
     1021 aacagctggg agtccacagg cctctcactt gggagaggcc cccaaggggg cccgctgccc
     1081 actgctcact ccccattgtg cctctgatga cgtcgtctca gaagctccac actaattctg
     1141 tgttcacacc gctagcagct acatcggtgt tctactttca gtggtctcat tgcaaggtgt
     1201 ggtgccaaca gagacggaga ggtggaatgt catgcagttc tggtttgtga ttggctgctt
     1261 gagagggatg actgccgaaa gtttactgtc agacaggata aaggagcatg agctgtggct
     1321 ccaactgcga taacaggatg aatttcaact tgcaagacac aaaagtgaag ttacagggag
     1381 ccaatttctg gcaggtttct ctgttctccc tctgccacca tcacaaattt gctgaattca
     1441 tggatggatt catgaatggg ctaacaaggc ccaggcccag ggtcacaaag agtctgagct
     1501 cccaaaaaat gtgcctgttc ttatatttct tatttacaag agctcagtct tttaatactg
     1561 agtttctact aacagttaag ttagttattt ggctttaatt ttgaaagaga gactgacgtt
     1621 caaatgcagt tt
//
LOCUS       KCVB01000351            1526 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16347pri ultra-conserved element locus
            uce-1062, sequence.
ACCESSION   KCVB01000351
VERSION     KCVB01000351.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16347pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1526
                     /note="ultra-conserved element locus uce-1062"
BASE COUNT          429 a          262 c          304 g          531 t
ORIGIN      
        1 agtacgtcct attgaaaaca aaatcttatg tgctgaccac ctcatgctga acagaaaacc
       61 ttcatcaaca gatataatac atactatcat tcacggaagc aatcacagta aagctgttgt
      121 ccctggacaa gatggcagaa ctgcctcatc gggctcattg gcccgtcttt tttgtttgtt
      181 ttcattctga catttggccc aatagaaatt aaagcaattg atttaacttg tgctcttgtt
      241 ttagcaatat gtattatcat aatgctttta atttttgaca tattacctta aggtcctttc
      301 caaaggtcct cttccttcag ctaagtctgt gtgtgcagtg tgtgactgga gtataggtga
      361 ataggaccct ccggccctgg ggcccagggc tcgatgtggt tggatgtcag ttaaaggata
      421 tctatctgat ccaaataacc gatgaatttg ctgtatctgt ctataaagtt gaggaagcac
      481 tagagaagtg ttttcctcta gttagaaaaa ttgtttgaat caagtcgatg gcagtcggtt
      541 acattgtgta tggaatgctc tacctggctt gtaactgact agaactgtga tgtacagatg
      601 atgtattatt aaaatctgaa gcaagtaatg caatgatatt aggatacagt ttttgtattt
      661 ttattcttgt ataaggttat ttgatggcta ttgtttagcc tctagttcat tctgtgttat
      721 ttaaattcta atatatgaat catatttgga ttgaagtcat gttcaggggc catgttgtgt
      781 atgtattgag atgtaaagcc tttgaatgtg aataatattg taaactataa tattttacag
      841 cttttttctt actatatcat acattttcta tttgctcagt gatttaatca tattctgata
      901 atttaatgaa tctgttcatt ctctctctga ttatttgtgc gctaggtcag tagatgttga
      961 atgatgaatt ttccacttaa atgcttggta gtccagtaat taaagcatgt agggatttag
     1021 gcatacaaat aaagccatgt gtcctgtctt ctgttaacaa ctacagtcat gacacagtga
     1081 aagcagcagc attcagtggc aatggcatct cacattttgc agcatgtaaa taaagtaccg
     1141 gcccattatc tgtttttgtg tcgtaaaaat tatttataat aatacacact tttattgcaa
     1201 acatcacatg agaaaacgta cagctggttg gtcttgggaa ctttaaaatc gcattttcta
     1261 tgtatcaatt attaagtact ctacacggac acaaattgta tgaacatact gtttgtcacc
     1321 ataactcttg atatgttgca ttcgcatccg ctaggacatt tccattctcc agtatcaaga
     1381 aatcattgct ccatattgga gcgtcagtag atcaagctgg gaaggacctc ctgtctctgg
     1441 aataatttat gcaagtgtct gtcagtgctg gtggagtggg ttagtaggag tggctgaggt
     1501 actggcagcc tgttttgcat ggactc
//
LOCUS       KCVB01000352            1488 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16393pri ultra-conserved element locus
            uce-738, sequence.
ACCESSION   KCVB01000352
VERSION     KCVB01000352.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16393pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1488
                     /note="ultra-conserved element locus uce-738"
BASE COUNT          486 a          305 c          260 g          437 t
ORIGIN      
        1 aacatttaaa gtattgtaca cagaactttt gttaattatc taaaatttcc acaaagcaaa
       61 gaaaaaaaaa cacatgaaaa agtatatcat attaattcta caatttaaaa ctgtaatatt
      121 ggagtatcat ggttaaccta tgactcccac cacatattat ggacaaacag atttgtgtat
      181 ctatgattaa aatttggttc aactgctgca ttcaaggcct aacatgtttt cagaccgacg
      241 gtatcagata caaaggcaat gtttaaaatg aatatttcta cagtttttca gtacctgact
      301 tgaaacgtat agtctaacct gtaggttggt acacgaaccc attatagtaa ctaaaatcag
      361 gtcatattct accggctgta ccgagcctca gacagacaac atctttaagc cttctgctct
      421 cacccacaca gtattcctgc tgtatagcac agcgtctacc ttcaccctct ctgccttctc
      481 tttctgtctt tctgggaagc tccaggccga gaatagagct cagagctcta tggtttacac
      541 aaaccacaat gccttctcag cttggagggg agggagaaag acacgagcat ctgtttttgt
      601 ttgcatttta ttccaaaaca taaaattgaa tcaatctttt aatactctgg attgtataaa
      661 catgggagcg aactgcttga ataaatatca agaatgaaaa tggatttctc gttcgacgac
      721 tattatctat gtggtttatt tatgaatacc acggctggtg ccaaaatagg cagaacataa
      781 aaatacagag ataatgtgtt tagccttttt tcattttctt ctttttaagt gaaagaataa
      841 atattaggat ttacatcaca tttacatttt tatcaaaatc aagttgggcg ccactaaacc
      901 tgtatgcacc attttcatcc ctggttgcca ggacaactgt gaaaataaca acagcaacat
      961 gacactgaca tctttaacag gaaagtcatt aaaagcaaca tgaggggcca gataatgctg
     1021 tctgtgccac agtcagtctg cgatctggat ccataaatag actaacggct ctttcactgc
     1081 tcccactaca acacactcat ttaggaaggt ttgtgggtgt tattagtaaa aacgatagat
     1141 aaaatcctct caatctcaga gactcgtaat agtggtgtga aatatcagac acagtgtgag
     1201 acgcataaaa tacacacgaa aaaacacagc acacacacct aaacatatcc ctgggccatc
     1261 tatcgctggc cacacaacac ccaccgacgc ttaactagtt ctcatacaca catatattca
     1321 taaggtatta tgcattataa cagttttatg gattttaaat ggcattcatt tacgaacact
     1381 gtggctagct atcatatgga agggctattt tatcccctct taatgtagat cagtcttctt
     1441 cggtgagatg cagagggagg tccaggacat tagggaagtt ggatctgt
//
LOCUS       KCVB01000353            1371 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16434pri ultra-conserved element locus
            uce-1283, sequence.
ACCESSION   KCVB01000353
VERSION     KCVB01000353.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16434pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1371
                     /note="ultra-conserved element locus uce-1283"
BASE COUNT          384 a          298 c          282 g          407 t
ORIGIN      
        1 attaagaagt tattaggctg caactaatat attagaatca accatattga ctattaacat
       61 gttagcatcc agaaaaaaaa tccttgtatg tatgaggcaa atttaaatta aatttgcgga
      121 caaataaaac atgcattgct actgaaaaag tggtaactcc tagtcatcgt gagccaagcc
      181 atattttctg aacgaaaagg atttaataca atgtaggctg tagaacttgc tgcagtgttt
      241 caggaacaga agcatgctgg ggggaggggg ttgctttcag tcacaagatg gcagcagagg
      301 atcataaaag tataacatat agggaagaag tttgatgctc tgctccattt gtaaatattg
      361 tagaggcttt agagaaaagg ccatgctctc agctgattta ttttcagtta attctgtttt
      421 aggtcctcag ctcttgttaa tttctacagt gtcagcacac atttgtaaaa acttacagta
      481 gcagctggat ccatgtttgc tgcagtgctg tttttgtgtt tttctacctg taccggctgt
      541 gatcctaggg cctcttattg cgaagaattt ctgcggcagt atacttgctg attgcttcgt
      601 tctcactcct cacaacaacc agctaagaca ctgccagaga aacacccaca ggagccacgt
      661 ccaactgtcg gtccctccgt ctgcctgtcc tcagcaagat ccccaatagt ctttgccgtg
      721 gcacttccat cggtgagtct ggcactgcag atgctcacac ttgcccagct ttgttgtgat
      781 gatggtttaa agacaacagg ctcacactaa aatcccccgg ggggtggcgt ggtcctttgc
      841 caacagctgg aagctctata agagaagtag gaaataatga ttggacatca gttagctcat
      901 gaaaattacc ctaatttgtc ctgatgcatt tgaatgtaaa atgaaaataa atatccgtgt
      961 ggattcactt tctttttgga atctcaagtt aagatttaaa gaggcactct cccctgccca
     1021 acgtagcttt atataactgc gatgcattga tgagtctacg ctccactgcc caaagtgacc
     1081 caaagcttgt tcccaaacag attcttgata ttcctctagc ccttttttgc agcatgactt
     1141 taagtgcaca ctacttggac aaacacaaac acccccgaac taagaataga cttgccatga
     1201 ctcatgattt tgcaacaaaa ggctcagttt gatttagatc tcatctgttt gcgcgtgtgt
     1261 gtctgcgtgt ttctttctgt tcacatgagt accatataat gcaagtgcct gtgtgtttat
     1321 gtacctaagg gacaacaact aaagccccct cgctatcagt tgcgtttgct c
//
LOCUS       KCVB01000354            1529 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16478pri ultra-conserved element locus
            uce-108, sequence.
ACCESSION   KCVB01000354
VERSION     KCVB01000354.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16478pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1529
                     /note="ultra-conserved element locus uce-108"
BASE COUNT          519 a          328 c          319 g          363 t
ORIGIN      
        1 cagaaaataa aacatgtttg attgtttatt gattacaagt gaaacaaaga ggagacagca
       61 gcatgaaaag gtaattttaa tacaatagta ggctgaagaa gaggtaagga gagacagaga
      121 gcataaacag aaaaagagga tgtaataaca agggaaggca gagataggaa acagggtgtc
      181 ttattccaga gagtggtgga ctgaagaagt ctacagagac agagcatcag taatgctgat
      241 atgatacaga ggtttccagt gtgactcccc acggctcgtc aggtactgaa gataccgaat
      301 acagctggct ggaatccaag agcaacaact cattgaaaag caatctcgaa ccgtttcaac
      361 acgcagggac ggtggaagta atggctgttg atagaggctc tgcgtaacta ctctgagtaa
      421 ccaaatccac caacacatcg actacaaaag cgctgtgcag aagagtgcag atcaaagcag
      481 acacacacac acacagttac gatagaaact gacaattcag gggccagtta aagaaagctt
      541 taacacacac agacaggcaa caaatttcca tcttgcagcc tttgtgtggg cgagtaacac
      601 tccctgctcg catgccagtg aatggagatt gctggattgg tcagggcagc gagagatcct
      661 tgtcggtgac agcatcgctc cagcgagctc gaaaccacag taacacagcc gaacaatcca
      721 aacacacccc accaattttt aatttcttcc attagaatta attatgaggc cgaaggcaat
      781 ttgtgtaccc tgtgtttaag actgcctgct ggattaatta aaaactggat gctggaaaat
      841 taatgtgtga attgttatgc ctttctataa tgagccatcc catgctatta cataggagtg
      901 tactgtacga tcaccttcag gcctccgaac acacaaggtt cattaaagag cacaaatcta
      961 gtcacaagtc aacttaaaag atgccgacct cagcacagta tcaacagagc acacacttgc
     1021 agcaagatat tcatattttc acccaaggta caaaaaaaag gtaagaaagt ctcgctttat
     1081 gacttctaaa taaatgaagt ttgtgacctg cgctcggatc tgataaaaca agacagagga
     1141 ctgaaaccac acagatgtca agttctgctc tgaaactgct ctcagcttaa aaccaggcaa
     1201 aacacgctgg ttgtttttgg ccgtgttccc ctgtctaata gagtgtgaag tacagtaaca
     1261 ttgtgcaata caaggcaaaa ctcttgggtc tgtcaccggc gtactgacca gctgcacaat
     1321 aacaccacag acacgaacac ttgacctaac ttttcaacct tccactgtag ctgtactcct
     1381 tatgtcagtt aaacctctct agatttacat ttggtttgtg tctttttttc agactttccg
     1441 ctgtaaaaga tagaagcaaa aagctcagta tgtcaatcaa agtacatcac aaatttgaga
     1501 tcaaaaacta caataaacaa cttttctct
//
LOCUS       KCVB01000355            1530 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16524pri ultra-conserved element locus
            uce-625, sequence.
ACCESSION   KCVB01000355
VERSION     KCVB01000355.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16524pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1530
                     /note="ultra-conserved element locus uce-625"
BASE COUNT          433 a          238 c          263 g          596 t
ORIGIN      
        1 taagtggaaa aatctaacaa tctaaaatca ttgttaagat aggtagatac aggatataaa
       61 tcttcttcta atgaagttta actttttatt tgatgccaaa tctcaaagtg attaaaatct
      121 gtttgacata attgtttttg ggtctcagct gaacaagcag gaaaaaagac atgcgaggca
      181 ccatgacaaa tcagtctttt ggtgctttaa atttactatg tacacaaaga ttcaaacaaa
      241 aattctgaaa atgaggatta tctaattaag ccaaattact tttacttaaa cagtatagtt
      301 gggtccatcc tagcatcatg agattttcta aaaaatagat acatattcta aaaaataatt
      361 ctgtttttaa tatatatata tctatccata tttatctctc tctctctctt tctctctgta
      421 tatttatata tatagatata tataaaaaga catatatata tattagtttc ttaaatgcag
      481 cctttgcctg tgctttatca tcttacagtt cctcttattt ctgtcagtgt atgtgtattt
      541 tttccttttc caaagctggg tcagctttct aatgctctca gatggagtgc aggttttggg
      601 ctgtgcagct attttgctgt caggctcttg tgttgaaagc agcgtctaag tgtgccaaag
      661 ttcaatttta tttcttgttt atttcttgtt ttctcattgg aaaatgggag cagctctgcc
      721 ctggcaacaa aagtaggcta tttgcatact gcgttgtgat tggtctgtgc attgatgggc
      781 ttgccaaatc acgctgtatc tctgtggtga taggctatgc taattaggtg ctgttgctgg
      841 gattctgaat agtggttact gtgtagcact ggtgaattgt gttgtctggt gagagatgct
      901 gatagctgaa catgctattt gataattttt tgattccttc tggcttaaaa tgaaggatga
      961 ttcactgtat agtgtggaga tgctgtattt tcaccccttt tcactgttaa acctgctgtt
     1021 tttttccttt ttcttcacaa aggttgatat cttactgtta tattttattt tgtggttatc
     1081 atttgatgca tttttatgac ttctcatgtt taacaaaaga tctgacattt attttaacaa
     1141 tcaaaaaatc cttgttattg tcctcaacat cttgtgataa tgtttgtctg ttttaaatgg
     1201 ctagtgtgtc atttcaacat acatgtaaat attacatata cataatttgc attttgcata
     1261 tatttagctt tcccaaaggt aaattcagac atttttgtgc taaggaaatt gtcactggtt
     1321 agattaagca ggaagtaatg cttatttaca ttagatctgt acaataagta gcaaatatct
     1381 ttacctcttt tgtttttggg ccaaaaatct cttgtgtgaa caaggaaaca cagctttttt
     1441 acatcaagaa tgttactctt tttcctcttt tgcgtgtgtt gttacattta atgctgaatt
     1501 aaggtagtat tcaactcaca gcagtcttaa
//
LOCUS       KCVB01000356            1431 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16559pri ultra-conserved element locus
            uce-1182, sequence.
ACCESSION   KCVB01000356
VERSION     KCVB01000356.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16559pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1431
                     /note="ultra-conserved element locus uce-1182"
BASE COUNT          431 a          284 c          338 g          378 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtacgtgag tccactggga caaaaaaaat tatagaaact
       61 gggggctttt ggcattagat taagtgtgtg ttcttccacc ccagcccccg ctccgtgccc
      121 tccacctaat ccccctccct tcaagaccag catgtggatg tgcggcgagc ccaccatgct
      181 tcagtgcagt ctataatgaa atgcttatat ttacaggcac agctgagcat gtcagacaga
      241 gcccctgcaa tctccccaaa taaaataacc aaaaataaat aaataaaggg ttcaaccttg
      301 tgaggcggag cgacttttaa agggatttat cagaatggaa ttgttcaaat gaaattccga
      361 ttcagacact taaaatatga ggcaacataa ggcaatatgt gctgtcggtg gctttgaggt
      421 tgcaggagtt tgccacggcg gagggagatg gtgtgaccac acaggtagct ggatggcaga
      481 aggtacacaa gcacagaaat cccaggaaga aagacagtca gatcaaattt agaatttcac
      541 caaagcagtg tctctctctg tggcacggtt gctattttga ggttaaaaaa caaaagaggg
      601 gggaccaaat cctcaaagta aaaataaata aatgaaaatc aatagtggtt aataaaacat
      661 caggtgagga gacataactg gtcgtgactt ttgctctgct caggcgtgca tgtgtgctgc
      721 tggtgccaca acgattaacc atctcgctga gagtggccct cttaaaaaat atcaacagag
      781 gagaggagcg aaaatatgta agagtgggaa taaaaagagg cgaacacaat ggcgcctggt
      841 gtcttttgat gtgcctccct gagaggaaaa agcaggagaa ggtgagaaaa gaatgggctg
      901 cctttgtaga ttgaacagag gggatgaaaa ccaactttga agaggacatc tctcaaatgc
      961 gtacataaac aatgtgtatt ctgtgcttga gataggtgaa cttgagacga aaatgccctt
     1021 gtggctggtg gattataacc tttaccttgt catggacggt catgcagctg gcggcatcct
     1081 tctggaggat ttcggtcacc ccattggaga gcctctcata cttcagcttg ggctcctcct
     1141 cgctgtcctc ctcctggttc agaaagcaga ctttaagatg tgctcttact gtagcgtttt
     1201 cttagaggta tttattacaa cctctctcaa acgttattgt ccacaatgtt ctttgaaagt
     1261 tattgacaaa aactcttcat gcagtaaact gaaatataca aaatgttcac tacaagtcat
     1321 gtaaatcctt tgttttgtct taaaatacaa tcttaaattt ttttttgaaa caagtgagaa
     1381 gctgcaacgt aactttagaa gcaccctgat gagtttttca gccactggtg g
//
LOCUS       KCVB01000357            1480 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16602pri ultra-conserved element locus
            uce-209, sequence.
ACCESSION   KCVB01000357
VERSION     KCVB01000357.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16602pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1480
                     /note="ultra-conserved element locus uce-209"
BASE COUNT          400 a          351 c          287 g          442 t
ORIGIN      
        1 tttttttttc cttctatccc atcctcaccc cctcccccaa cctcgccagg caccttgtcc
       61 gcatctggag aaggcttgta agtttcgggt gcatacattt cttttgatga tatattgtcc
      121 cggtgcggaa tttcatttac tgccgctgcc ctttgctgaa cctgttgcta gtcagtgagc
      181 acgataagcg tataagtagc atgctacttc attcatcata catcgttctg ggctctcaaa
      241 tatctcatat gcgattcagg aagaaactac taaaccggca caaaggatcc caccaggatc
      301 agctgaagtt ccattaaaca ctgtgtacaa aatagagcac acttggtagt tattatcaaa
      361 tacacatttt cagcgttgca tctacaaaac attagccctc atttcatggt gtacacgttg
      421 tttatgtagc agggggaaaa tgtatctcaa gtgcagtgct cttgtagtac ataattccat
      481 tcacattgca ttttgaatgc atgcattttg aaatctgttt tttccctcaa tcatagtggc
      541 tgctctgtgt tgtattaaac agcattaata atcacaagag gctgagtgtc actcagaagt
      601 ctccacatca ctaaggcaga cagtgttgtg ctgctgggca tgttgtgctg tgtgaaagtg
      661 cttacacgct ccctcagaca accaaaggga ataattaaag acagaagatg gaggcagaag
      721 aatggaaagc aaatcccatt gtttggggtt atttcatccc actgaatttc tttttctcac
      781 tttctttcgt tctcttctcg cgttcctccc caaatgcact gtgggtatgt ggcacgctat
      841 caaaagcata tgtaagcgaa gcagcgcaga acaaaagccc tgtgatgtcc tcctcatacc
      901 cacattgtct tttgattatt ctacaatcat tttatatctg tgtaagttgg aaaggaaggg
      961 aaggcttaat aaccccataa tatacaatac tttagctgct cagacaccgc aatgcaagat
     1021 gtgttttaga cgatttgaaa acaaacttgc tcattcttta ttcgttctct ttttctctaa
     1081 tggcgtgata tcctcaagcc ctcattaccc aagttgttac caaaataagc cacaataaaa
     1141 ttcaaattta aggtgaacta gactattagg ccaactctac tccactccat ttctagagca
     1201 gtaaatgtag cccctcaatc tctctcagct tttactgtca gtcagagagc actcagtcat
     1261 ctccgtgcat caggcccgca atattgattt ttccacccac tgaaacagcc gcatgaacat
     1321 aaattttgca tgaggctttt actcgcggcc tccgctttgg tctgacgaca catgatggga
     1381 ccatgaacac gctgtaataa cccagtctgt ccagacaagc tctttttcct taaggaaggg
     1441 ggtgggggtg gagggggtcc agcagcatct ggactctgaa
//
LOCUS       KCVB01000358             967 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16650pri ultra-conserved element locus
            uce-524, sequence.
ACCESSION   KCVB01000358
VERSION     KCVB01000358.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 967)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16650pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>967
                     /note="ultra-conserved element locus uce-524"
BASE COUNT          246 a          228 c          227 g          266 t
ORIGIN      
        1 cttattcgcc cctctggtgc cagcctgcac tacaacacag agaagccaag cataacacat
       61 acagcattcc agaggcatca atgctgagaa taaatgatat gacctctggc tatagcatgg
      121 ccttcaaatc taaatcccta cctgaccagc aagaatcatc ctcctttgtt cattcttcct
      181 gcgtaccgaa tacattctgc actgaatcac atctccatga atcttttgat tcctttcaat
      241 cagtttcaag gactgtaaac aatgggcctg cgaatgtgat ggggaaggaa atctacctca
      301 gctcaattaa ggagctgccc tgcagcctcc tgcggccctg agagatagga gctataatta
      361 gaagagggca gttaacttgc agacattatt gatgatccac tcctgacacc tccctcatgt
      421 tagcattagc attttccctg agctggggct catgtcaaga gaggctggtc tctcagagtc
      481 catgtcagca ggggggctat atatgagcta cacagcgagg cctctttatg gctgcagaaa
      541 gggagagaga gggagagaga gagacaccgg ggctctcctt caggcccctc ttggtgctcg
      601 tccacctgat gggaacacgg acacttggct gttctccccc tcatcaatac agcagggctc
      661 tttgtcagta atttgcaata agtgtccgtg ccacatctta agggacctgg aaatcattac
      721 tggctgaggc cctactctgc tactaattgg atgcgttggg ccttatcaac tgaggatttt
      781 ccgagacctt tgacagatac caattcgaaa atcattgttc agttgtcaat ttaagagact
      841 gttaaaagag cttattttaa ttttaattgg aacttcctcc tgccttcaca gtgtgctgca
      901 aggattaaaa atgagctcag ggcatgtgtg tatgtgtgtg tgtgtgtgtg tgtgtgtgtg
      961 tgtgtgt
//
LOCUS       KCVB01000359            1925 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16697pri ultra-conserved element locus
            uce-561, sequence.
ACCESSION   KCVB01000359
VERSION     KCVB01000359.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16697pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1925
                     /note="ultra-conserved element locus uce-561"
BASE COUNT          515 a          431 c          435 g          544 t
ORIGIN      
        1 ctttgaacca ttttttcttt gctattctgt agttactgct ctccgacttc tttgacttta
       61 agcagtgtta aaagaggaaa aaactggtca ccataaatca acacaacatc agttcattta
      121 acgtatagcc taataaggcc tgtttagtgt tgtcctctca aacacggcta tgcaatcttg
      181 tatcactaaa tttagaaatt tacccattaa ttagtgtgag tctctgtatc tgtgttcccg
      241 catccctctc tgtgcgtgtc attaaaccat ggacaggaca gcgcaggagc cattgtcagg
      301 tcaggctcta caaagctacg gctgatgtag gtggcctacg ttgcctcatt agtaacaaca
      361 tgagcggtgg tctaatgaac ttaagacaat gctaattaat ggaagagaac aaaggcttaa
      421 catttttaga aagtgtgaag cactgagtct gcttgtatga cagcgtttaa gccaccttaa
      481 tccgcttgtg atgcggcgca tggtgcctcg acccctcagt gctggggcag gtcgcaggga
      541 gcagctccat acctcagctg atatagtcac ctgtgacaat cctttatggg ctgataaagt
      601 aagggaagtc tctcaaggaa cagtgcactg acttggcgga gggtctcccc agggagcagg
      661 tgtcagggtt gtctatcaga aggctgaaag gagaaaaggg acgtgttacc gggctctatt
      721 gtatcccggt tgtcattgac tgatcgggtc catttagctg gctttcacag tgaatgaaac
      781 cacctgaata gatgaaagcg agacagcctt cccgacaaac aaggagccct ggaatattca
      841 tggaggatct gtgaggaaaa gattaatgta ttagaaatta aaatggggcc cggacgtatg
      901 atcttgctct aacacttggc cagacctgtg tcacccgccg ctagcgcctg aagtcggcac
      961 atgccgctgc caaggtggag aggaatcatt aagcaccctg ccattgtgtg agcagaataa
     1021 tcaaacttca cccccccatt ctttcccgca gcactgacaa tgaataattc tggagccatt
     1081 tgcaatgtta acggcaccta tctgggccat ccatgccttt atgataagag ctccaaatat
     1141 gcatttcaac agtctgaaca gaagctgctt caccggcatt tcaaaggaca cctgtcagtc
     1201 atctgaaaga gctgtcagtc tctgtcacgc aacggtgccg gtgaaatggc ccaaaagata
     1261 atctttactc tctctctttc tctctctcca cctctctctt tgagatcaga gcagttacat
     1321 ttgcaataga gtgacaagtg atttacactg gaaaatggat tgtgtttaaa ctgagaaaaa
     1381 aaaggggggt ttattctttt aggctgcgtt actctttgct ggtgtacagc tgtatcaaat
     1441 atgctatcat aaatgtgatc tgggctacac tgaatgtcat tatgtgtgat accacgagca
     1501 ctagacgggc tactgagctc aaaggtgact ttgataattc ctatctctct aagcagagct
     1561 ctgtgtctat gaggagcagg agatggggtg ggatcaaaca ggtttgtgtt tccaaaaagt
     1621 ttgaccttct gttgttttta actggaaggt gtcttcaaat agcgatcact gctgtgtggt
     1681 tttatctgcc atgctggaca tgaaccgcca agctgcagta acctgctata gattttttct
     1741 gacttaaaaa aatctctctt ttcccagcat tacctcacag aaaagacttt ttctttactt
     1801 tactccacaa accagtttgt tgcagattat acttctactg ctgcttgttt ggcaaggagg
     1861 caagtgaagc cccctgcccc tccacatgta ggccagtaat ttgttaagca cgactaactt
     1921 gtgcg
//
LOCUS       KCVB01000360            1401 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16740pri ultra-conserved element locus
            uce-271, sequence.
ACCESSION   KCVB01000360
VERSION     KCVB01000360.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16740pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1401
                     /note="ultra-conserved element locus uce-271"
BASE COUNT          404 a          277 c          294 g          426 t
ORIGIN      
        1 aaaagatttc acacaggcaa ctagtgtgat aatatgttta ccctggctgt gtgtgtgtgc
       61 tgctcatagc tgtgggtcag ttaagagcag tgtttaaatc ttcctcccca ccaaggtaac
      121 ttgtttgttt tagcaggaaa aagatgcatt tgacatcttc tgttgctgcc attacttctt
      181 tcagtgtttt tgtttcgttt tgtttttatt ttcatttcct ttcatggcta gtgcaaattg
      241 gccttcccag catcagctta gcggcatggg aacccaaaca agcttgtata atgcattggc
      301 caccgctggc accgtggcta gcttgagcgg ctaaaaggaa aaaaggctgg ctgttgtctc
      361 ttctattctg ctctgcaccg ccattactat tgtacaagca agcaggctca ttacagaacc
      421 atatgcttgg taacgatgaa agctgtgggt gcatcccttg tatgagctgg gatgcttaaa
      481 ttattaaata caccgcagag gcttgattca gatgtggcaa gcagcccagt tctctctctc
      541 acacacacac atacacacaa agaaatgaat aaaggcgtgg ggaaaaactt caaacacctt
      601 tcctgtgcat ttagtgtaat gaacaatgcc ttaattgaga ttggctaatt acccaatttc
      661 aacataatta atatgatcct aattagcata tgtaaatggg acgttgcata attgagaatc
      721 cctgttttga attagatggc tctaaagtga acccgcacgt gaaaaggtac aaaacagcta
      781 ttaagagttt gatgccacat taaaacgggc catcattcaa tgtgcgctga tgaaacgttc
      841 agcatcacgc agatgttcag gagcattatg gaatgtagct gattattgtt gatatgtttt
      901 tgggacatcg ctaattagaa gtcattacct acttatcact tttgtgccac tacttaagca
      961 ggaatgaaaa caatcttggc gctcgagttg agttcacgca caaatggcaa gcgttggtga
     1021 tgtgcacatt cagcattcag ctcatctgtg tccttggagc tttgtcagca agtgttcctg
     1081 cagccaagat gtttgcaatg aggtggtaca gttattataa tggaaaacgc atcttcggat
     1141 gtgtgcattg ctgtaatgca aatgttgctg ttaacagcat acagtatatg cacctgtcta
     1201 aagcactggt tggccatata atgccagcta ataaagaatc aaaacagaga aaaagagaac
     1261 atttgttact ttaaaacaag cattccttat atatgttcag ttcacatttg agtccgaagt
     1321 ttttcctttt tgatctaaaa ctgtcttaac acacaagcct cgtgcagcaa agagatgctg
     1381 aacattgtga tacagtattg t
//
LOCUS       KCVB01000361            1696 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16779pri ultra-conserved element locus
            uce-1243, sequence.
ACCESSION   KCVB01000361
VERSION     KCVB01000361.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16779pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1696
                     /note="ultra-conserved element locus uce-1243"
BASE COUNT          421 a          420 c          271 g          584 t
ORIGIN      
        1 gtttgtctgc aaggtgagac aacatttagt gttgctctca cagcagcaca acaagtaagt
       61 gccgattccc tgaccctctc tgacagtgag ctttcaccct acaggttagt aaatagttgt
      121 agctgaaaat cccaacagga taacaaacag tttagggggt tagacgctaa gagccaaggg
      181 ctgcttgccg tctttctttc tttctttctt tttacagatc agtctttcac agtcatttcc
      241 tttcctcttt gaaaggcaca ctttgttaga ctaggtgtga aaaaacacta caaattttgt
      301 tggggtttaa gccatcctac acaaatttta tcaaaaagtc cctcgtctta cagtacttgt
      361 tttctctgtt ttctttctcg caggactctg atgtagaaaa tttggctagg atggcaagac
      421 ggctctttat tactgcagtc gatctctgac aaatcatgta gtgtttttga acagcaacac
      481 taactcatcc atgataaaag tcattttttt actaaccaat atctaaccaa ctaatgtact
      541 aactaacatc ctaattcttc atcgttgctt ttgttttttg ttttctgtcc atccttatta
      601 acaacctttt ttgttcacta ttcttttgat ttcctttctt cccctttttc tggaaagcaa
      661 tcaggcattg gacacgctat ggtaaacaaa ctacattgtc tggtagatat cattcttatt
      721 gtcttttttt tgggtttgcc gtgtgtcccc tccccctctc ttcttttatt cgttctcttt
      781 gagttccatc ctagacactc ttatcaaaaa atgaggagat gggtttgtat taccaggcaa
      841 ttctttcttc gcttcttctc aatttcctcc ctgacctgcc aacaacctcc ttctttaccc
      901 cacaatcccc catcctgttt tttttttcac tttggctttt atttttttct ctttgtttct
      961 tccacattac tgcagatttc caacaagtaa aaaaaaaatt gacttctttg cctttgtcag
     1021 ggcaacttac ttttttattt ttattttttt tcacacaaaa agtccccatt ttttgtgagg
     1081 actgtcgtcc agaactgaca gaagcttacc acaatcccaa agatggtgac ctcgacaccg
     1141 agccctaaaa gagaaactac caaaaaagtg gcaaccctta acctttattg cgggagctct
     1201 gacagttgtt tccaactcac tatggcgcat ggcatgccca cacttcattg acctctcacc
     1261 atccaaactc ataaaacctc aaccctgtca ccaataataa ccactaacac tctaattcac
     1321 tcactcacct tccacctatc accgtatcac ttactctgcc ctccctgtgt gtgttttttg
     1381 tggcatgcta gtgaaagtgg tgttggtgtg ttctggctaa ccttgatcac ccttacctct
     1441 gggcccatat gcataacatt tctaagaata agcatactga tcaaggatta tcggtctggt
     1501 ccagtgacat tgaaaatata accatgacac ctgagctaaa cctgatcaca gatcagcatc
     1561 agctattctg agagtgtctt tgaatatggg ctctggtctt gatcgtgcat gagccttcgc
     1621 actctgtgtg gcctgctctg gctttgtatg gcaccattga tccacctact cccttacatg
     1681 tccaattaag tgtgtt
//
LOCUS       KCVB01000362            1399 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16817pri ultra-conserved element locus
            uce-1206, sequence.
ACCESSION   KCVB01000362
VERSION     KCVB01000362.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16817pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1399
                     /note="ultra-conserved element locus uce-1206"
BASE COUNT          438 a          264 c          320 g          377 t
ORIGIN      
        1 gtgctcttcc gatctaatat tctgctaaaa aaacaattag agggtaaagg aagttaaagt
       61 ggatgctaag tcttaaagat atatggtatt aaaacaacaa ggcaaaaagt atgtgaatga
      121 aaacaccatc agttgtaagt gtctctttga ggctcctgtg tttgctaatt tgtccatata
      181 gatttcagct cattccatcc ctctaatgct ccattctgtc aatagttggt cacagtgttg
      241 tctgttcctc actgtggtgc atttaatatt taccagagtc tgagccttat taaagcaaga
      301 tgaattagcc tggttgggat agattttctt tcagggaaga gtgaaagaaa gaaaagtaga
      361 aaagaaagat aaaggcaggg aggagggagg cagaggaaga aagtttgtgt gctcagatga
      421 tcagcatgcg ggggccccag ctcgggagcg gcccccagcg ttggaggagt tgtgagctcg
      481 ctgatgatgg ggttgcacag atgggctcga gcctggctcc cagctcgctc cttttgtatg
      541 gaccctgctc tctaattaca gctgatgagt gtggggagag ggggaggaaa aggggaacca
      601 gagtagggaa gggtgggggg ggcatacaag caggtgacag tacgaggaga ggaacctgtg
      661 gcagatcctc ggcaccatca ggcacaacgt agccgcagcc tgacagctaa ttagaaacga
      721 cacggatttc tcccaatgta acagatgttg cctaatcagt ccagctcggg agaaccctgc
      781 gccagcaaca tttcatgcac aatttaacca tctaaaaggg atgaaatact taatgcattg
      841 taaggccagc acagctgttt ctctcgtcag aaaatttccc agtcaaaaat atgcatgttt
      901 tatgtaactc taaccgagtg tttataatgc tataatagct gagaaaaaat tgctgccaca
      961 catctgccaa agatttcagc tctattggtt accataacat cgatggagca aaagaccaaa
     1021 tgcagatttg acttttttga gacttgaata gcttgtctca ccctgacaac aaatcaataa
     1081 actttcatac aaagatcctt gtacaaaatc agcaactgcc attttaaatc aaattgaaaa
     1141 ggtttagttg gataaaggaa acacattaat acattgttac atatttctta tcaaaatact
     1201 ttaatataac atcttataaa tgaatttatt gttaaagttc cagactgcag aagtgcttta
     1261 agcaatacgg ttacacagta ttttgtattt tggtgtcagg agcctgaaga caaaccataa
     1321 aggccaattc aggcagcgac atgttttttt ccctgcaaac tgctaaatta catgtgtatg
     1381 tagcgtgtgc accatttaa
//
LOCUS       KCVB01000363            1476 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16856pri ultra-conserved element locus
            uce-135, sequence.
ACCESSION   KCVB01000363
VERSION     KCVB01000363.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16856pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1476
                     /note="ultra-conserved element locus uce-135"
BASE COUNT          400 a          356 c          292 g          428 t
ORIGIN      
        1 aacattatta tatgtaagct accaggatgt tccggtgtta tcggcgaagt gttctgcggc
       61 taagtcagtg gtctttgtgg tttaatgcac gtgtattcag tgggatatat ttaagggaga
      121 aaggcagaga tcataagcta cccctacagc tcccacaccc caccccaccc cttcatccaa
      181 aaccatacct atcaccagca ctggtgcccc cacaaaaggc aaaactgcct tccaccccct
      241 tcccgtttta cacaagctat atggtggatt aaggttttgg tggcctggcc agcagggcct
      301 ttgtagatgg cttagcacat aactgggggt ggtggattaa ataaaatgat cacagaagca
      361 aagaaatgat acaggaatta cttatcaaaa tactagccca ggttttatct gcagcttaat
      421 tttgttcagt acattctcag ggtgatgttc agattaattg aactgacagc ttcctttcaa
      481 atttccggca aggtatttgc acggatttca ggtcttacac aaacttaatt atatggaacc
      541 ccattttatc attctaattc catgtagcgg gggttctatt tacaaatgac aagtctgtgc
      601 cttttattca cagctttcct taatatattt atcaaagcgg gttcatttac aaagtgctct
      661 atctgttggg tacaggcagg ccgacattaa cgaagctttt aggtttatca gactcgcagc
      721 ctgacaagcc acccgagggt caattgattt tttggttctg tgatacattt tttttctcat
      781 tttttctgga tcacaatgag aaacatgcct ggagaattag cctggtttgt cggccttatc
      841 tatcagcccc ctccctcgtc ctcccccctc cgttctgaca tgctctctct ctctttctgt
      901 ctatctctct ctctctctct ctctctcacc cttttcttta ttctcttcta gccaaaggaa
      961 gtcaagctac ttaaattggc cacaggaacc gctgttatca gactttaatg caccctacag
     1021 tgtggatagc atttcaatta caccagtaac caaagattag cccccgcttg cctgtttcat
     1081 gcctcgtgcc accgtttcgg gacgccacgc accttaccta caggagcaga aaattgagtt
     1141 gccttgcctg tgtcatggtg attaatgcag aaatagtctg caaacctgca aggagaggga
     1201 gagagagaaa tagacagaga cagagataga tataggaaga gttcctgcct tctcagaggc
     1261 catgaaatcc aagcctggcg ctgaaatgcc tcagatccat ttataaagag gtctgaaaga
     1321 gtttgtgctg ggtaacatcc tcatgaacct tatttccgca cacacgcaga cacacacata
     1381 catgtaggtg atcatgaagc tgtgtattaa tgtttcacca gccgtcctaa caaaaacaca
     1441 ccaggaatca attttcacaa ataaagtcat tagaga
//
LOCUS       KCVB01000364            1631 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16900pri ultra-conserved element locus
            uce-976, sequence.
ACCESSION   KCVB01000364
VERSION     KCVB01000364.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16900pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1631
                     /note="ultra-conserved element locus uce-976"
BASE COUNT          481 a          303 c          342 g          505 t
ORIGIN      
        1 tgctgctctt tttttccatt tttgaggaaa cacaagtggg agtgtgcaga aaatcctaga
       61 tcacttcctg tcttgtggtt taggcttatt tatgatacag taagaaatca caaacattta
      121 aaatcacaaa tatgatagca aacaaaacaa acaacaacaa aaactaagta ggaaaacatc
      181 ctttcccgct gtcctggtca taagaaatgt gcctgtgacc tggttaaggc cccagtccag
      241 gattttacaa accaagctgt acagtggtga tagaaacacc atggattcag cctctgtact
      301 ctaagtcact ttggttaaac acacctgctt aaatttccct gtgtgctgtg catgtgtatg
      361 tattttggct tgcatgaaaa tagatttttt tgatgttagc catgccacct tcctacgcta
      421 attagcacgt gaaatccagt tgacaagact agacaaaagc ttgaaaaggt gtttgtgtgt
      481 tcggtttggg tgatgccatt tcatgcaatg caccatggct ctcaggatgt atcattgctg
      541 agacacaaaa aggaaagagc attagagggc ctgtctgctg tacactagtc tgttctggtc
      601 tgttgtgttg cgttatggtg ctagcctggc ttctagccct agcttgagtg ctctctccac
      661 tctaccattg ttccatttga tagcactaag tgactgattt aggttcaatg cctgcatgga
      721 gcagacaggc cagctgtggg ttaagcagat agcattcaag tgagctaacc caattcaaac
      781 ccaatcaagc tgggatttat tgttggggtc tgtatttaga aatctgatag agtctgcatc
      841 agtcccttga cttgcatcag gagagatggg ccttatttct tttagcggag gactataaac
      901 ggcaaaccat gttttccagc agggacataa atttcaggat ttgtagacat taccgcaaat
      961 gcacacaaag aatgggaggg agggacaggg gcacaggaag tggggatggc acagctacag
     1021 acatggctcc atggttcaca aatatagtct gtctgtcttt ctgtgagaag atgcgatttc
     1081 acacctaccg attcatacca tgaccttttc tgaatgcaat ataaaagcaa tcagtactta
     1141 aatacaggcg cctgtggaaa gcttttcttt ttaaaaatct actgtgactt gtaataaaat
     1201 ttgacttacc tgtcaatcat ttttattcaa gtgtgtgcaa atataaagtc tgtcaagaga
     1261 gggatttgga atttgtttgc tgttgtgtta gtttaaggcc aaaaaatagg catcattgcc
     1321 ttagcgataa aaggataaat gcatcacatt ttaagagata ttcttttgtg ttatatttaa
     1381 aaagtcaacc atattcatac ttaaattagg atttaaaatc acatctaaca gctaaaaaga
     1441 tggtagacag tttctgggaa ttaaatagta attttctatt gggtgacagg ctggtctagt
     1501 aatagctatg tctgagattc ttcagtgtat tctatgtatt tatctcacat gtccttgact
     1561 gtcacaaata caagcgtctg acattttcct tcattttgtg acatgtcgga tataaacaca
     1621 gtgtctaaag t
//
LOCUS       KCVB01000365            1524 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16945pri ultra-conserved element locus
            uce-933, sequence.
ACCESSION   KCVB01000365
VERSION     KCVB01000365.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16945pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1524
                     /note="ultra-conserved element locus uce-933"
BASE COUNT          371 a          355 c          353 g          445 t
ORIGIN      
        1 gtcttgtttt caccttttga gctatgaatg acggcgtgct gatgaagtta gtggccgctg
       61 ggctgctttt tagtgttttt tctttgtcac ctacatttat attttctctc ttgtctagac
      121 tgaactgatg ctatatgaat gttttttagg tcttgtaatt ttaaaagtta aaatcccttt
      181 gagacatatg atcaggataa gagcagggtg cttaagtcag agtgaacaaa gaagaactct
      241 acattgttat tatctctctg tttctgccac aaagactttc agacagaaat ctgctggtgc
      301 actcagcagt ggagtggaaa aggcccttca agcgcgtaga caggcctctc tgatttggtc
      361 aatattgtgt catgtttggt caaaccccgc caaggcagtg ggctggaaat gaagatgtaa
      421 atattgatat ttcattagag gaaatcaccc actccgacgg cacttaaccg ggccaccttc
      481 agagtgcggc ggccggagcg cagtgtgacc tgcggagtcg ggaagtcact tcattactcg
      541 attaaattga gtggaaaaag gcgaggcaac gacacgagat cccatttgga gcgataattt
      601 aggccctgaa gagaggggat gaagcagggg aaaaacacca cctctccatc tcacaacact
      661 ggattaaatt caaggacttt tagcgtcaaa ggtaattgta ggctgctggt ctgcatgtgt
      721 gcatatggct gagaggtgtc tgctgtatca gaacaactct aaagcctctc caacaagccc
      781 tgaacaaggc cttcacacgg gctgtcacat tgggtgcagc gctttagttc cgcttcagtc
      841 ctccttggca ttaaaaggct agacaaagcc tcgcctgtga ttgtgatgcc aacctgatgc
      901 ggcaatcaag actgcgtcca cgcttgcatg acaatttcgc tccggccagt ccacagtctg
      961 tccaacttaa tggccacgta tgcagacaag gaaataacac aacaaaagat tttaagatgc
     1021 agcctggata tgtgtaacgg cagaggcctt tacaaaatat gccttattgc ttccgctgat
     1081 aatagtcaaa tgttttgaga gctttcggca gtttgcttgg ttgggtctta tttgggctct
     1141 ttagacctct gaccagtcca gcagggcacc agcacctttc tgtcagcact ctattctact
     1201 gactattgat tccccttcaa aatgtgaagg ccctcacatt ctgatcacaa tctgttaatt
     1261 aatgtatcct ccagctcttc ccctgcagtg gtcatcacca cgataaggga aattgaacac
     1321 tcaggtacta ctctccccgg ctcttctttt ctctccgcct gtctctctct ctccgtcctg
     1381 ttttcttttt cactttcttt cacgcttcct catcgtgtct cttgttcttc taacaatgtg
     1441 tgtatgggtg tgtgggtgtg tgtgtgttca tggtgtcctg gcaggaggag ataaacatca
     1501 ggctggtggc tgcaccccag tgag
//
LOCUS       KCVB01000366            1572 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 16989pri ultra-conserved element locus
            uce-618, sequence.
ACCESSION   KCVB01000366
VERSION     KCVB01000366.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="16989pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1572
                     /note="ultra-conserved element locus uce-618"
BASE COUNT          447 a          295 c          347 g          483 t
ORIGIN      
        1 atcctctgct gtctctctaa caaggagggt cagtgtgaca gacatacagt atctctgtct
       61 gagactacga cacgacaaaa aggtgacatg gagcagtggc agacagtggt ggcatgtttg
      121 tcacccatcc atccacctca tccagggtta aaagggcagc ttgctccctg ccggaggtac
      181 gtgtatatta tgggcaccct atgcaggcta agccgcagtt gtgtgtcgtg tagactgtta
      241 tgggtgctgt gtgatactct cagcgtaact caaataaatc acctcattag cctgttctct
      301 aatgggttat tgtaacacca agctgacacc cttctcttgt aaaagctcat gagatttgaa
      361 aattcaaaaa tgcttttggg tgggatcgtt ttcctatcct ctggcacctg gtcgaggtca
      421 ttctattagg tggattgatt tacatataaa ttgaacatgc agtattgcgc acctccttaa
      481 actcatctgt ttcactactg tatcccatta tctgccttgc atactccaac aagcatttaa
      541 tttcgtcttt catattgtct tatgtttcat tatgtttcag tgttatttat taaaattcat
      601 ttagctaata acgcccacac gcactgcccc ttttcctgca acacacctat tctaatgggt
      661 tctgagcgac cgtgtgtcct gaggtaaaat gggaaatgtt gaaaattcac tggaatatga
      721 aaagcaatca tattttctgt atcagtaagc aggtctgtgg tagctggggg tcattcttgc
      781 tctctactgt tgcttgggga ggcatttagt aagagccccc aaggctcacc tagttaatta
      841 agtttttctg ctattagaac gatgtaactt tgattttact gtgccacaaa gctaattctt
      901 tgctacagct gaggatttaa tagacgttac ttctcctctg cacgatgcct tgtttatttg
      961 gagtgcattg tgtcgtgaca tgatacatat caatgatatt tattatagga ctcatacttg
     1021 aggtgaaaat gatgagatga tttgtggata catgcattga acagtacctg gccctaccaa
     1081 aaatgagcct gtcagtaaag taaaaaaaaa gaaaaagtgt tgaaagaaag tgaaaataaa
     1141 tttgctgaac acgtgaactg tgcagcggag ttgtcttggt gatatggagt ttgaacgcta
     1201 tgaagaggtg gaagggaaga tccagatata gaaacatgtg ggtgtgtgta tgtgacttta
     1261 tccactgcaa agaaataaat taagcagtaa cactggcagg gtgagacaca tatgaggact
     1321 gaaaagacag actgattcaa agctggatgg aaaaaatgta attgcatggc tttctgctgc
     1381 aacagtgctg caccattctt gccctctatg agcagactca tttgaggatt tttttaaaaa
     1441 ttgttcagta ttttgaagtg agaaagatgg cctcctaaac ctggtgtttt tcaagtcatt
     1501 aagggttcat gaaccagcag tagcctgtgc tcttcttaaa ggaaatacat tttttgaatg
     1561 aggaggaaat aa
//
LOCUS       KCVB01000367            1145 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17032pri ultra-conserved element locus
            uce-1142, sequence.
ACCESSION   KCVB01000367
VERSION     KCVB01000367.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17032pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1145
                     /note="ultra-conserved element locus uce-1142"
BASE COUNT          320 a          248 c          227 g          350 t
ORIGIN      
        1 gtttggaggg tggggttgag ctagtggttc ttaaactttt tctggcatgt tcccttcgga
       61 tgctgaagac atttacagcc cctccccaca atgtttatct gtcattaaca atgacagagg
      121 aagcaactaa caaaaacagt aagttaaatt taaatgacat aaaagtaaac aagttagtat
      181 cagcaaattt ttgtttattt tctcttcata aatcatattt tttcatctta gcttcactcc
      241 acaactttct cctggtcctc cacactccac ctgcaggggc tccgtgcccc accagtgtgg
      301 cctgccccac agtttgagaa ccactggttt aaggtgttcg atggacactg gtagtactgg
      361 ctgtttatca taaacacagc ctagcccaga gaggtccatc tgctttgatt ttatttagtg
      421 attcccccca gggcccctga gctggagcga gaataacaat acttgagccc atctgttcgt
      481 gtagaaccag ccccggctaa attaccacac gctacgagcg gctggcatat gctgtggcat
      541 aaagctacaa gagggcattg tgtgccagct gttctgttga caaatactta tctggcactt
      601 ctgaactcag cttgctaagc tggtttcaag tttgactaaa aactaaacgg ggtaaggtgt
      661 ggaatagggt tcctgtgggg gggatgcatg tataatacat gctgatttat ccctgggtat
      721 cattttatca gacattgtct gggaaattgg gtcactggat gtagacctaa tctgaacata
      781 taggccagat agaagactaa agccaaccac acgggtcaaa acagactata cttactgtaa
      841 tctttctcat cctcccgtaa gcacgctgtg atcatattgt tgttgtatgt ttacaagttg
      901 ctggatgatc actgtgtcag aggcctaaat tgggctcgat catagtaatc ctatcagtaa
      961 agactcagat cttcatagga gtgactaata cacatcttaa acactgaaaa atacaccaca
     1021 aagcctgttt ttacaaggat ttactgctat ggccttcaga tactcacagg aactttatga
     1081 ttctcataat actcattctc attcttcttc ttattattat tattattatt attattaaat
     1141 acata
//
LOCUS       KCVB01000368            1032 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17071pri ultra-conserved element locus
            uce-1107, sequence.
ACCESSION   KCVB01000368
VERSION     KCVB01000368.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17071pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1032
                     /note="ultra-conserved element locus uce-1107"
BASE COUNT          281 a          184 c          343 g          224 t
ORIGIN      
        1 cacgcttgag tctctcacct gaaggtgtag tgcagtctag tgttggtgct taaacaccag
       61 tgccagtgcg cactggagca gaaaagacac acacacagag aactcctgac agcacggtgt
      121 tgtcagaaca caaagccgtc ttttccctgg gccccttggg aacctgagag aaatcacagc
      181 catctccagc cgtcaaaccc ctccactggc ctctctctgt cttctgtctc tctctcagtg
      241 tctccatctt tctttgattc tgtcctcctg tcactcaata tttttgtttt gctcatagat
      301 ttatggatgg ttaaatggat acattgatgg atagatttat acacagatgg atggatacat
      361 ggattgataa aagagggtca caggtagatg ctggagatgg tcagcagcca aatgaggaag
      421 tgggtgaatg tagaggagga attggagagg tgggacgtat agccagagag aagtgaggta
      481 aaaggagggg caaagaggca gcacattggg gttgggggtg ggtggggggc attggtgggg
      541 tgaagaggag gaatagaggg atgagggagg ggtgtaacgc tgagacgtgt cagagaaggg
      601 cgcctgggcc tccctgtgga atgtggcctg tctgcctccc cagaaagaac agagcaggag
      661 agagggaaga gagagggaag aaagggaaga gggggcgaag accagttctg ccccatcatc
      721 gaggggagag agagagagag aaggaaggga gggaggagag agagggaaaa caaagagcag
      781 agttacaggg cgagaggaag gagaggtgga aaagtgagag tgcagtagag agtgtatggc
      841 actttgttga ggtgccgggc acgggtgtag acatgaaatg atgcatagca gaggagaaat
      901 gtaagcagga taaatcctaa caaacctcaa agtttgtgtg tgcgcgtgtg tgtgtgtggc
      961 tgagtggagg gtgctctgac gagaaccagg tgtcttcaat tctttcgttt tctttacgtc
     1021 tctcatctcc tc
//
LOCUS       KCVB01000369            1756 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17138pri ultra-conserved element locus
            uce-3, sequence.
ACCESSION   KCVB01000369
VERSION     KCVB01000369.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17138pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1756
                     /note="ultra-conserved element locus uce-3"
BASE COUNT          592 a          276 c          315 g          573 t
ORIGIN      
        1 ctttgtttta caatttaagc aaatttggtc atcagagtgt cttaaaggtg tggtggatga
       61 ataagattcc aacaattcaa taagataaaa taaaagttta atgaatatca atgaatttga
      121 tgcaacacaa cattgcactt tttttaaaca actaaaatgc tttgacacag ttaaaaaaca
      181 aaagattcca catatttgct ctgctctaaa tttagctgtt ggtatcgatt tcttcagttt
      241 ttgtgataac ttagctgtca tagtggcctt cttcgacaga actgtgtatg tgtgtgtgtg
      301 tgtgtgtgtg gacgaggagc tgagccccgc cctctgtcaa acaccaacac agatagccaa
      361 ggacagaagc agagtgacac aatgacacta aaatatatag aagcatattt aattctgttc
      421 atttggctta gacatggtaa aatatgctta taattgtagg gaaaaccatg attttcataa
      481 attgcatcct tattttggca aattctgcaa taatcctata tacagttgat ttagttttat
      541 tatcagattc tgtaattccg tccgtgtatt tcacattgta gaaatcatag gaccgaaatt
      601 acggatgttt tgttgtacta aaatcatttt tttttcttga gttacaattt tctatacaaa
      661 gcttgttaat gtgaatataa cataatatgc acactaaaac atgagtgcaa tatattttat
      721 gccataacac aaactttgtt ttctcctgct tgattgctga agccgaagcc ttgacacttt
      781 tgatgtactc tgccaagaac attaagtatc caaaagtgat aaagcttcaa tttgctagac
      841 cattagacta attactgcaa taattaagta actacttgat tagaactaat gggtacctaa
      901 ttatccagga agaaaggatc aggtggtaat taatagagtc aagctgatgt cttctgactt
      961 accaatgccg attaccaaac atacgggact gaaactggtg aggacagaaa tgaaaaaaaa
     1021 ggaaatgcca tttgagtgtt tacctaaccc cctgtagcaa aggcaaacag tttgctggta
     1081 tcacaccaca tgagcgcatt gttaatgatt gctaatgtaa gaggaacatt acacatcaat
     1141 acattataca ggatgattag cctgcatcta gacaatgatc tctatagtat tgatgctgat
     1201 tacagagata aagaagtatt attctgtact tgacacaggt tattttgatg gatcggtgac
     1261 atttgtttcc atttatgctc aagctttgat tttttttctt tttttttttt gtgtatggat
     1321 aacgctatgc acaggaagct gttcctcact ccaagaaaat gaatgatgta aacttgattt
     1381 taatcacatt tcacaatgaa catccctcac agacacagag agagatgaat agagacaaat
     1441 ctaaaattta tagaattcta caatttgcat aaagtgtatt atctaaacat aagtgaaaag
     1501 taagggggtt gaaaaggtca ttaaaattta actatattga atacagaata tggacattat
     1561 ctatcaatcc atcaatctgc tagttactta atgtataatt gttgtaagac gggcagcagt
     1621 caagcctggt ctacatataa tatgcatgac tatgaatgga agtaaaggtg aagactgaca
     1681 cctttagatg tatagttctt ttttatatta atgtttgaat aatacgtgcc tggagggtgg
     1741 gccataaatt aaaatg
//
LOCUS       KCVB01000370            1629 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17194pri ultra-conserved element locus
            uce-740, sequence.
ACCESSION   KCVB01000370
VERSION     KCVB01000370.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17194pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1629
                     /note="ultra-conserved element locus uce-740"
BASE COUNT          427 a          350 c          391 g          461 t
ORIGIN      
        1 gatgccacat ttatgataat cagagtataa ttcatttatt gggtctacct ttgccatgtg
       61 ttgaccccac ggccatgttg ccagctcaca tctggctgca tggtgcggcg ctgagtcctg
      121 gatgggattt gcccctggtg atggggcatc tgggaggcca gatgaagctg aggcttgatg
      181 agtgctttgt ctattatgga tatggggctt tatggagaca ttgacagctg cacattgaca
      241 gtctcctgag caggaggcct acttcagcag ttgtgctctt tctgttgtct agaacacagg
      301 gaacaaacac agagctctga atattagctg gattctccgt tagctactgc aggttttaag
      361 tgaaaccttg atttggaagg aagagcaggg atgatatagt ttttaattta ttcagtctag
      421 tcatgaacct tttaacacag attattgaat tgataaaaat gttaaatctt cagtcaggag
      481 aagcctccag accacacaca tgtgaaagaa atattttcag taattgcagc ctacctaaca
      541 tcaggcattt tgcacatatt taccgctgct ccccaaagtc tgcatggata agatgcaccc
      601 accagtatca atacatgttt ctacaaatta taccaaaacg aggctgtaac tgaatgaacc
      661 tatcagcaca tcccttgtat gccccggcac tcttaagtga taggttctgg gtcggagcgg
      721 tggatggaga gagcttgacg acgaacgcaa atgaaagatc atccaccgac aagagacatg
      781 tagctacaga ccaggcaagc cgagcctgac gttctccaac cttccctcct gcccgccatt
      841 tgtgaaggat ttggggattt cataatttcc tgttctttct gaaaggggga tggcagcacc
      901 tggtccacac agctgtactt catttagaga gcaatttgaa aagagcgagg gggagaggag
      961 aggagcggag cagccttgtc attaagggct ccagaatggc ctccgtttgt tagacacatg
     1021 gcacagagtt aatgctgctg aatggggaat aggcgctgtt gatgctgtca caaatttctc
     1081 ataagtgcta cgacatctga aaaagccact ccaagtgcag ggagggggga aattcaaatt
     1141 ttccttatca aagaggggtt tttttgtagg taattgacag ctgcgactgg gatgtaagca
     1201 tgtgtttctg tctgttaaga tggatattct atttgtgtac tatttcatct ttcacacacc
     1261 ttgaagtttg ttgtgcatgc aggtgatggc agtgctagag tttgtttcaa tgagttgaca
     1321 atgagaataa taaccagatc attggtaaac taaggtgatg caaaggtttg ttgtgcggat
     1381 gcgcagctgc ctcgcctcct cagagttgat ctaattgatt ttgacgtgca catgtgcagg
     1441 ggaaaaaaaa ttgtgaggcg ggatcaaagg caaacgcttt attgccacat cacaggcccc
     1501 tgctgcattt tgaattctac caatctgtcc aaacagtcag ataaaatcct gcgatgaaga
     1561 gatgcgctcc tctctctctt ctctctcttg ctcactctct tcccctgcct tcctctctct
     1621 ctctctctc
//
LOCUS       KCVB01000371            1501 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17234pri ultra-conserved element locus
            uce-705, sequence.
ACCESSION   KCVB01000371
VERSION     KCVB01000371.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17234pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1501
                     /note="ultra-conserved element locus uce-705"
BASE COUNT          437 a          290 c          322 g          452 t
ORIGIN      
        1 tgtttatcat gtaatattct aggaaattac gaaacattaa ctgtgagaaa gggttttatc
       61 cattagtata attatgatct gactttatac acgtgagatt cgaggtgagt tctcagagat
      121 ttcattgaca taacattagc aaatctctca cttaggcaca tctctcctgg cttgtaaaca
      181 ggcagagtca cagtgataag catatttccc aaaaataaca tgtacaggac tattatattt
      241 aataacatat tgacttgtac ttttatttag aatgctgtat tactaattga aaaaaataag
      301 ctaaaaaggg atttgcatta gaagaagaga gcaaataata attttattat tgaccagcaa
      361 aatcatatat acataaatta attaagtcct ttttatgctc aacaattcat taacacaaaa
      421 taacattgta ttctggtcac agtgtatgaa tgtgattatc tcagaacatt ttttatagca
      481 tgaacaagag actatttcca agtagctata ttcatgaaaa aaaaaaaaaa atcttaaaga
      541 tttcaagcat gggaggaatt tttacatttg ggaggaggat gcaatcagta gcatgggact
      601 tgcagaggcc tcctcgggtg acaaattgaa agtgtctcgg tctagtttat agccctgatc
      661 aggtcaaacg aaaaatattg gaggtctggg tgaggagaca ggcccgcctg gtgcagataa
      721 atggactgat catttcttat tgagaaacaa ttcctaaaag gtagtttact gtgtgactgg
      781 ggaagcggcg agcggagagc agaacacgca cccactgctc cctccctgac ccttcctcca
      841 ctgctggaat taaatggata gagagaacgc gtgtttctga tggcactcct ctgccagaga
      901 gacactccat ttgttatgaa gtaggggttc tgatgaagtg tagggagctc ctctttcttt
      961 tttcccccct tttttcatcc ttcctttctc aggcactaca aggccggcct tcagtctgct
     1021 tcacatgaaa tggatggaag gagccgattg aattttacac tcccctggtc atgattaatg
     1081 tgtttggtgt ggggagtctt ggcagtgaaa ctccactacc actagctggt gctcaagctc
     1141 tccaatcgtt gtagcctaat aactttgtag aggagtgggg ggtggggggc ggctgttaaa
     1201 tcggggcagt tttggtcaag aaaccggtcc tttcttagcc cacaacccct tcaactcctc
     1261 tactcttgtg ttaatagaaa atctcagctc ttgcccactc gctcattatt acttcagagt
     1321 cagtttctca gtgtgctctg tacccagtgt tacttagcag gccgagctgt aaatgtcagt
     1381 ttatcactta gcacattgct aaagggtgag aggtctaata aaagatgcca ggtcatgtta
     1441 tcggattccc taacagatgc tcatggtgtc ggttcatact ttgtgcgtgg acctgaaatt
     1501 g
//
LOCUS       KCVB01000372             953 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17276pri ultra-conserved element locus
            uce-441, sequence.
ACCESSION   KCVB01000372
VERSION     KCVB01000372.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 953)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17276pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>953
                     /note="ultra-conserved element locus uce-441"
BASE COUNT          212 a          271 c          210 g          260 t
ORIGIN      
        1 cgcaaagaag ggcagctccg accccaacaa atagaactga gttaaagatg attgatgtgt
       61 ctcagaaaaa tccatcccat aatttcacct tttcaccttg gaaaggcttc ctcctaagac
      121 ccatattaaa gcttaattct gccaacgagg agcaggcggt gttttaaaga gaagaacaca
      181 tgtctgtgag gcctgttcct ccttatctag cagcttcagc cacagccaaa gatagatgtc
      241 tgccatgcca gcatgtttat tttccttgcc gattagcttc gattaaaagc agaccatgtt
      301 gtctctctcc tcctatttgt gcattcgtgt gtctgcctct gtctgtgtgt gtgtgtgtgc
      361 atggagtcct ccgatcctcc ctgtgcgggt gtgggggtgt ttgtgtgaga gtctccgtta
      421 tttcatctcc actaggctgc acatgataat taagtcatgg ggggctggga gagggaaggt
      481 gggttacagc ttacagcttt gagtaattct gtgccctacg tcaccctccc tcccaaccca
      541 cccttcctct gtctccttac agcctacctt aaccctcccc tcccgctcca catccccctg
      601 cacctcggct ccagtcccac ctcaggctgt gggaattttc tttagtcaca ctcggcttac
      661 caggtaaaca agcctgaatg ggggagacgc gagcagagtc catgggagca aataaaacag
      721 gggaggatgt aaatggattt atcactgagc cccctcaccc gcctgcaact ccctcacccc
      781 ccacttcctc cacccgcact ctcttttctc ccgagcgagg tgaggtagca ctctgctaac
      841 ttggcagtct ctttaacgag tcaatcccag acctctggaa ctcaggtgct cggagagctc
      901 agaatcggcg aagtggtgac tttttcttta aatatttttt ccattcactc ttg
//
LOCUS       KCVB01000373            1184 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17296pri ultra-conserved element locus
            uce-404, sequence.
ACCESSION   KCVB01000373
VERSION     KCVB01000373.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17296pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1184
                     /note="ultra-conserved element locus uce-404"
BASE COUNT          326 a          217 c          313 g          328 t
ORIGIN      
        1 tttgttaatg catcactgaa acctaaaaca catacagatg tgtttggggc aaaaaaccac
       61 aaactcaact gagaggattg ccattgtttt ctagtgcatg cttgtgctta atttttatcc
      121 gcttacttat agaaattttg caacaaatgt gcgactttta cacacacagc ccccgtatta
      181 aataaattta ctttcctccc cctcacagtg ttaagggtca agctgtgtta gtgtggtgat
      241 ggcggttttg gccctgggaa ggcgggacag ctgtatgaga gaggtagctt cagtagatgg
      301 ggtaaaagtt gggtgggaga gggatacagc atcatttttc acctcatgta ttgcatatgt
      361 atttgtgtga gtgtctgtgt gtgtactgtg tgaacgtgtg aggctgattg ctgatatgtt
      421 tgtgtttatt gactcaaaga gacgggccgt ggctgcgtac acagcggtgt acaagtggca
      481 ggggtccagg cttgggggaa acgtgtgtgt ttaaagtaca cacaggccta attatgctga
      541 tttaagcatt cttgcggtcc tccctctggc ttgtggtgag ttaactggca caggggcgtc
      601 cagcagagag agagagagag agagagtgag gtacagagag caagggtaaa gatgaagata
      661 acagacagac accaaaggct caataaagac aatgattaag aaaaggaaga agaaagagaa
      721 aaggtggtat taggctgtaa cacagtgaga gtgactatga ggagaaaaaa gctgtcccag
      781 acactggtga aacacaggat aaacatacag ccgccgtcaa atttcccggc aggttttttc
      841 cctcctcact ttggggttaa gagcgcgccc gctcttcgta tttattttta tattcagatg
      901 ttatggattg ctccgggaag aggccgtccc ttttcccagc aagaggaatt aatactgttt
      961 gaatgttctc tctggagtct aaaaggaagg atttttgcaa tagagtttgg gaggtttata
     1021 agtgaaaacc tgaccactgc taaataaaca aactagggat gattaaaaac tgacccctgt
     1081 ttgttgactc cctgcttttt ggcctgtgtg tgtgcacctg tctcctgtcg ctttgtgcgc
     1141 atgcatatac cgcacagtta acactgtgtg tacgcagaaa gtgg
//
LOCUS       KCVB01000374            1502 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17380pri ultra-conserved element locus
            uce-1140, sequence.
ACCESSION   KCVB01000374
VERSION     KCVB01000374.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17380pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1502
                     /note="ultra-conserved element locus uce-1140"
BASE COUNT          482 a          286 c          316 g          418 t
ORIGIN      
        1 ggcactacgg gatcaaaggg aaagcgtgga accctaaaga agccataact ctggggttta
       61 attgtgtttc gagtaaatgt cagagcaccc cagacgtctg ggaatcatct gcctcccttt
      121 aataccagtt attgtcattc tatcaaacaa aatcacattt ggaccaaaat atttggagaa
      181 gtagcatgtg ggggattcac tccacgcaga gcagtaaatt agagaacaaa tgtaaacggg
      241 gagctgtttt atgacacgca aagtaaacat gctgtaaatt ggattgtcat tggctcattt
      301 ctttaaaaag cagcagagaa atgtgtgctg gatgttaagg ggagaagttg tgttgatcat
      361 cggcagtaag attgcttcat aactagagtg aaacagagtg tctcgtgtgt ctgtgataat
      421 tagaactata ttccgttttg cataatcgcc cagaaagata aaaaaagttg agatgaagca
      481 ttatcaaaac aatgataact gcacaagggg acgttatcaa tatatctgtt tcacatgcgg
      541 agtgctctct catattttga caacctatat agcacattta accttcggtg tctgtttctc
      601 aaaatgaaat tgcactgtga aaaaaaaaag aagaagagaa agactcatat taccgtgaaa
      661 aagatggctt ttctctggaa caataagcag tccttgaact ccacgtcatt aaagtgaatg
      721 ccatcatttc ctttcattaa gatgactgtg tgcagacaca tattgcagtt ggaaggcaaa
      781 gctgcggcag gagactcatt taaaaaccgc cctaatctgg aaaatgattc taatgtttct
      841 caaatgttat atgctcccaa cctagcatgt gaaacgctta agagagctgt aacattttga
      901 ggcattttaa acgagtctct gcttaaaaaa tgtaccctga gagctgcttt ccctcctttc
      961 acatatattg ccaatatttt tgacaataaa ttttgaaaaa ctgaataaaa tccacatctc
     1021 tccccctcgt agattatgat tatcacacgc gaaaaggcct ctcataaatt gcgaatgctt
     1081 ttttgttgct gtcagagcgt agaaatagtc atgccctctg ctgttttccc tcatttgact
     1141 caacgggtgt gaaacagtcc ctcatatcct tgtcttgtct ataagagatg aaatgacttt
     1201 aagactctgc agtgttgcca caggcaggcc ggctgaaagg ctatttcagg agaaatggac
     1261 atggacataa atatcctcac ggggtgcttt gtaataaaaa aaacacaatg ttatgaaacg
     1321 cagtgaaaca taaagaaaga aagaaggagg ggtgtgtgtg tgtggggcgg tggaggggaa
     1381 atatccatca aattaacaca tcagtgactt tgattgtaat ctctcctccc tggctcttgc
     1441 tctgccacgc tagacaagca gccaaacgtg atttatcaat gcaaagctaa tggcatcaat
     1501 at
//
LOCUS       KCVB01000375             826 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17419pri ultra-conserved element locus
            uce-1105, sequence.
ACCESSION   KCVB01000375
VERSION     KCVB01000375.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17419pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>826
                     /note="ultra-conserved element locus uce-1105"
BASE COUNT          274 a          183 c          214 g          155 t
ORIGIN      
        1 ttcactgtct attggaataa caatggaagt gattggagct aacaatccga atcactaggc
       61 gcggggattt gaaacatatg tcatgttgtt gcgcatcagt gataaaaact aataacaata
      121 ttccctgact gcactgtatg agtgagagtc agacacaaag cgctaccaga gcgcagcccc
      181 cccaccgcac cccatcccgc aatgtcagat acatcagcaa tgctaatgcc caggcagcat
      241 cctgcgccgc tcaccctgaa ccactgaaaa gacaaccatt taggctttaa aagacacaca
      301 taaacaaaaa aggaaggagt agatgaaaag agaggaaaaa aagaaagaaa acgcaagagg
      361 gggagaggaa gaaagaatga agggaggcca gggtgggggg gtgggaggaa gtcaatttcg
      421 tattctgaac ctggccgatg tagtcctcgg ggatctgcct ccgcacctca ggatctgtaa
      481 ataattgaca gataatatta attggctttg gattagtgag caagctcagt atcacacatt
      541 aatatttgat cagaggatga tagggaccct ggcaggggat ccgaaggggc agctgaggct
      601 gttttgttga ttaggccaga gacgggagga gatggaggca agtggtagca gcagcaacag
      661 caacaacagc agctccagac acacgcacac acagatggaa acacacagcc gtggtttgat
      721 gctcccgtta gctccaggct gctaaacctc ctactgccag caaactgcct gctccttgtt
      781 tcgccacaaa gactaaggaa agaaaaaaaa aaaaccagag gaggac
//
LOCUS       KCVB01000376            1198 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17461pri ultra-conserved element locus
            uce-974, sequence.
ACCESSION   KCVB01000376
VERSION     KCVB01000376.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17461pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1198
                     /note="ultra-conserved element locus uce-974"
BASE COUNT          338 a          231 c          285 g          344 t
ORIGIN      
        1 ggcagctctc ctgggaggga ggtggaaagg agggggagga ctggagtaga atggaagctg
       61 gcaccacatt atctccaaca gttggtgcat tccttaatga tcaagtgctg gagcagcatc
      121 tgtgatgaaa gtgtgtgtgt gtgtgtgtga gagagaggga gcatatgtgt acatattagc
      181 atatgttcac tgtgtttgtt taattcccct ttacaccact ttaaaaagaa gtattttctg
      241 tgctttaggg gagaaaatgt cacgcactga tgttgttctg tttgtcagag gattatgatt
      301 tgaggggagt ttgttgcccc gggagaggaa actgtctcac ccaggggtga gatgcaggga
      361 agaagagctg agctctgcta ctcccccttg cccttaaccc ccgttttaag atctgtgatc
      421 tttgggccat agagggagtt aacacacaca aacacacaca acacacggag tcttttctca
      481 ctccctctct tagggtcaag ggcacaaact gtgagttggg aattggaaag tcaataaagc
      541 agaagtaatg ttataatgac cctgagctga agatgtcctg tcgaccccca tcacatcgga
      601 ggaagtggac aatcaaacaa aatggtcact ctgcttgatc tctctgaaag ccatccttaa
      661 acaaggccac tgactccctg ttacttccag ttcctctgaa gtcagtgtgt ttgtcaaagg
      721 gggaaatggt gtttggtttg tggaagcaga gggcaggaga tgttacagat ggataaaagg
      781 acacaacact tgtatacatt ctcaactgta taaatctcct ttgttagatg acgaggatga
      841 taacggtaaa actggaagag tccaaaacac tacatgtgac aggtccttct caattttccc
      901 tgtttctctg tttcacattt tgaggaatca aaaacacatg acagccacat caaactgaga
      961 ataaatgtct ccatgacatc actgacacat ttacataatc ataaattgtg tccttttgtt
     1021 gccagaacta tgatcataaa ttattaagtg ttagatatgg tggttggact attcattatg
     1081 gtgccctggg gctttgtggt cataattagt gggtctgttt tggttttgca ttttgcttca
     1141 aatatagaca cacagtttta caaactgtat catcctaagg cactatatga aacctaac
//
LOCUS       KCVB01000377             988 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17498pri ultra-conserved element locus
            uce-172, sequence.
ACCESSION   KCVB01000377
VERSION     KCVB01000377.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17498pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>988
                     /note="ultra-conserved element locus uce-172"
BASE COUNT          328 a          185 c          170 g          305 t
ORIGIN      
        1 gggggaaatg tgtgaaaaga ctagaagggt tttaaattgt tccacaagtg tgcctacaag
       61 tgtacatgtt gcaaatatga ggctaattta ttaaataaaa acccctttca aattgatgtg
      121 gacttaataa tatttagcat tgtctcttgc atattctggt atggtctcac tttaatccat
      181 aaagtatcaa aaaatttttt gaaaaatgcc cattaaaaca tttgagagtc caagcttatg
      241 tcatcaaatg gattgttgtg ttttcaccca aaattccaaa ataataaatt aagaattatg
      301 caaaacagaa aagcagtaac acttcacatt ttagacactg gagcatgctg ttgtaaacaa
      361 cattgacgag tcactgtagt ctcaccattg ttttctgaag taaaacaacc tctagaaaag
      421 aatattttac accatgtcaa gagtttattt gacagtattt cagaatatct ttcagtaaac
      481 aagttttttg ttttttagca aaatgtaata tatcagtact gtcgcatttc ataaaaatag
      541 ttgttcacgt ctttgaaact acagtatcaa tgatacagta aaccatattt gtaggaatac
      601 agctgtacaa ggtgaatagt acacaattaa cactacacag ttatacttga tctcaatggg
      661 gatttatgag cgtgccgtcc ttcacaggtt ccaataaagc aggttttttt tgttctgtat
      721 acaaaatcac tcacagcagg caggggcacg gcctggacgg agacggaggg ctggatgaca
      781 acttctttca ccttttctga actctgacat ttgtacataa agtaaaacac gtttctacaa
      841 cctagccgca tgttctcatg ctcctcacta acatgttaac cagcttatca catgaaccac
      901 agttgtattc aacagtgagc tcagaaacat ccagaccgca tgttttactc agaaaccaca
      961 gcgggtcaag atccgtcttg atgcttta
//
LOCUS       KCVB01000378            1243 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17526pri ultra-conserved element locus
            uce-1241, sequence.
ACCESSION   KCVB01000378
VERSION     KCVB01000378.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17526pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1243
                     /note="ultra-conserved element locus uce-1241"
BASE COUNT          334 a          256 c          271 g          382 t
ORIGIN      
        1 ataagcctcc tttgtaaatg gttaatgttt tgactcagtg cagaccagtg gactgaagaa
       61 aatgtttctt gtcgagacct aataacacac ttatgaagat cttgtttgtc tctttctgca
      121 agtccctgcc tatggcacag cctctattag gcccctgtta cgtttcttct cacaaatgac
      181 cgtttaaccg tcttttccgc accctgacgc ctcgcctgtt attaccgcca ttgtctctct
      241 ttctgctgta tacatgtgtg aggctgggcc ctttaatatg cggagagctt ttcctaggct
      301 ttcctacggg cacactggtc tgtgtttact cctcagtcag aatgcctcct ttccacaagt
      361 ttgacatgcc tttttgttgc catggtaagc cctcccttaa aatatatatt tctgcttttg
      421 gcctggatgg tttttttatc tcagtggttt agagcctgtt gcccccaggg ccagatttaa
      481 atgtgtgtgg gtcttcaagg atttctccgc agaaaagccg tttggattgc agcctcaacg
      541 ccatacaagt gcgaccgaat tgtgtcattt caggtttttg agccatcgtt ggaaccaaag
      601 gaaagtccaa aagagaagca aaaaacacaa gacaaaagct gatgaaaatg gaccagattg
      661 gattatgtca gacttggcat gtgtctcgtt atgagggaag tcttcgttct gtcttatcac
      721 actctgcctt acagctaaat gaatttaaaa aaaaaaggca aagcacagct ctgctccttg
      781 gaaaacaaca gcagatcgta actgtaaata ctggcttaaa gtggtgtggg ctccgcacaa
      841 ttttcagtca caaaacagga agtgagagga gggaaatgat tgcactaact ttagggaaat
      901 ggaccacttt taagcagtta tggaaggggc aatctttaca acacttgagc tgaaatgtac
      961 ccagaaaaca gtgttttttt tataagcaca tgtcgtcaca gacttggatt gatatgggtc
     1021 ctcatgtcta gagagccact acagtatgtt caagtgggtt caactctctg ggtgtatgta
     1081 tgactttctg tgagataaaa catctcatgt tcttcggtgg tttggatctt aaagacagaa
     1141 actgtgcaga tgcttcccat tgcttgccat gatgattttt tttaaaaagg aaaacattca
     1201 ctagaaatag ttaaatagtg aatgagtggg actattaggt tgt
//
LOCUS       KCVB01000379            1497 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17567pri ultra-conserved element locus
            uce-1204, sequence.
ACCESSION   KCVB01000379
VERSION     KCVB01000379.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17567pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1497
                     /note="ultra-conserved element locus uce-1204"
BASE COUNT          428 a          334 c          284 g          451 t
ORIGIN      
        1 catctcaacg cttctttgag ttcaggaaag tggtgataat tcaacatggt gtccatagga
       61 aaaacattcg tatttgatga ttttgcacct cttgatttac atccattgcc aacgtttggt
      121 gccaatgcag gaagtagtgt gcccgtttta gtgaaagacg agtgacgggt gtggtcgtgg
      181 tggattttta ttaactgtag ccataaactt tcgctaacct tagcctagta ctctaccaaa
      241 cctttatcaa agtttatatt tcataatcac aattgttccg tatttcaaac acaacgtagt
      301 tgctgtacac agatattgta aaaagcatta aaaaaacatt tgcactgaac taaaaacgaa
      361 caaacaaacc acattgtcgt tgattttaat atcaggtttt tgctgacagt ccaatactga
      421 catttctttt caacaattgc gttggataat ttaacgcaac atcatctagt ttaaattaga
      481 aaaacactag tttactatgt gaagtgtgga tatacacaaa cagaaggtaa acacatgtaa
      541 gtgagcatgt atgcacacat gcagaagtac gcactcgtca cacacaggtg tgtgtgtctg
      601 tgttgcagtg ttggtataac agggtattaa cagtctatat tgtatgtcac tttggcttta
      661 gcatagtcag tttcctctga ccataaatgt atgcctatgc acgctaactg tgtggaaaat
      721 gcatgcagcc tccacctagc ataaccaccc ctgctgcact cccctgtgga taacaaatgg
      781 gccttttctc tgggaattga ggctaaacga tggtttcact tagagagcaa agtgactgtc
      841 catcttcata acagtgctct ccatcaaaga gcaatgaatc ccatcccgtc ccactggctc
      901 tgattaaacg caggcaatgg atatcccctt tatgaaggcc tgcaaagctg gccctcatgt
      961 cagccttgca cctgcaacta tcagcttctc cttctcttgt aataaaaaag ccccacatct
     1021 ggtcagggca gcagcgctct gcaccggcag gggtcttcag ccaagtctgt ttgaaagtga
     1081 cacacctcca attgatttaa attacctaag gcatttatta ttggtggggt cacatggaaa
     1141 acccacaggt cacaaagttc actggttgag ggactttcac ccttggttgt gctttgaacc
     1201 ttccccctgg tcttttctcc cggttgtcat atgataatat catgttaaag cagtgtgctc
     1261 tctcattcac ataaggttat aatgtgtaat tgcattatga aactatcacc gtttcatttc
     1321 atccattccc attgaaccaa attattggtg gacatgagat gtctccactt ctcaaagaga
     1381 tccgaagctg tttaaacatt ctttgctgtt atcgagctga acaaatccaa ctgtctgtta
     1441 aacaacttga actcaatcaa gtccccatgc tgtccaaaac ggccttaaga tgtttct
//
LOCUS       KCVB01000380            1008 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17606pri ultra-conserved element locus
            uce-236, sequence.
ACCESSION   KCVB01000380
VERSION     KCVB01000380.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17606pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1008
                     /note="ultra-conserved element locus uce-236"
BASE COUNT          238 a          183 c          254 g          333 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
       61 tgtgtgtgtg tgttttaaag agagtgacaa ccccccttct ctctcattag cacggtatct
      121 tggtatcaga actgccagga agtgacagac cactgtaaaa gaacaggtgc tgttgggttt
      181 gagatgtgtt acagagcaaa cactccctca tcaaaccggg tgtctgttct gcactctgcc
      241 tctgtcttct gtcacatact atccaaatca cacagagtcg ccatcttgtc atatggtcat
      301 ctgaatgaag ctcattgtca gggaaggtat taatggcgtg tacgtgtatg tgtatgtgtg
      361 tgttttccag ggtggagaca ggctggctgc tcatcctata tgctcttgtc ttttttcctc
      421 cttcctctcc tctcctctcc tccttccttt cttctccggg agtgtgatta gcaggaggac
      481 acatggtgag ctgagctggg gaagggaagg gaaggggcat gagaaatcat taattacagc
      541 cgcgctggag ctgatgaagt aaatgtgaga tagtgtaaca cactggattt agtgaagtgg
      601 acgacagcac aagattaatg gtggggccag gctgatgagg atgaagtcac agcttagctt
      661 gataattagt ttccaagtgg aaacatccct caccattgtg ttatttttac ttctctcctc
      721 ttattattat accacgatga tatctgcaga acgggggggg gtacagttag tgatgttcta
      781 gcaaacatgt cgggcagcag aggtgtttgt tttagaaagt gatgcatgtc tgtagcattt
      841 tattttatct tttctttgtc tggtttttct ggtttttctc atatttatgt tacactttaa
      901 atgtaacatc cactgtgttt tctggaatca aggcttctgc agaagtggca gctcattctt
      961 ggtaataata aacaaaaaaa agcctcaaaa tttaaattaa attgaaat
//
LOCUS       KCVB01000381            1456 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17635pri ultra-conserved element locus
            uce-273, sequence.
ACCESSION   KCVB01000381
VERSION     KCVB01000381.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17635pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1456
                     /note="ultra-conserved element locus uce-273"
BASE COUNT          409 a          321 c          230 g          496 t
ORIGIN      
        1 cttacatttt ttttataaaa tgcagcaaat ttccagttgg ctttctgtca tatttggctc
       61 tcaattatca agtaagcatt tcttacactt taacagaatg cattgtgaat atctttgtga
      121 gtttatttga acaaatagtc tttcttcctc tgaggctaaa aacaatttcg gctactcacc
      181 tgatagttgg gaaaaatatt cagcgactgg agctgccttt tgacatatga cagatacgga
      241 gcctgggaca gaaaagacaa tgtaattctg agggtatccg tcctgataaa aatgcctttg
      301 gcaagatgaa gcgcagtgta ttgttacaca gcaatgtctt aagatgtgta atactccaga
      361 tgattttata ctggctgaag gggaactgag agtgctgccc ttcctctatc ctctgtcgat
      421 ataaacatca catttctatg tttttctctc tttcctctct cgctgtcttt cacgtacttt
      481 taaacacaaa cagactaaca ccactctctt ccctctaagc ctcccattcc tttgactcag
      541 tgccactgca gctgcctgct ctcatctcat ctcatcttat ctctctctct ctctcacaca
      601 cacatacact tgcatgcaca caaacacaca catacacaca cacacacaca accactgcca
      661 cccgcatgca tttatttatt cgttatattt cctaattcag tttgacgaat gagtgacagc
      721 ttgacacttc attcggcaag attaatagaa cgacacaata tttacgtggc agagacagcc
      781 aattatccca ccatctatca tccagattat ctttctgatt gaaaactact cgtccatgta
      841 atcggaatta tttggaatta gaagggaatt tatggcttct tgtgtcactt ttcctggacg
      901 gtggtaaatt agaaatatta gatacataaa gagagtatag gaaatgggat aaatagaggc
      961 ctggaattaa tgtcacgcgt ttccgtttta ttttttttgc cagtctgggt gcccctgaat
     1021 gaaccccatt tttgcctcaa tgttttatag gttatcttca caaattatta acataaatat
     1081 cctgttaata acactgtata attcatatct ataaagaaga gttgtcacaa tatttgattg
     1141 gtccgttttc tttgccaaag gatatagata tgtttgattg agccgtgagt catttttaat
     1201 cagaaagcag aattaaaatt tcaactttgg agacagtaat ggatagatct tatcaccaga
     1261 gatagctttg tgtgtattga tctctggcta tgtttttaaa tgtccatctc actcaagcca
     1321 gaaccgacat tagtttactc cttcaacctc tgtccttcag cctctgttga ttaattctct
     1381 cgactctttc tatctcaccc tacaatactt tgtattctgt tttcatacat cacctttttg
     1441 taccattctc ttctca
//
LOCUS       KCVB01000382            1058 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17703pri ultra-conserved element locus
            uce-96, sequence.
ACCESSION   KCVB01000382
VERSION     KCVB01000382.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17703pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1058
                     /note="ultra-conserved element locus uce-96"
BASE COUNT          295 a          213 c          213 g          337 t
ORIGIN      
        1 ccgcccccct cctccttccc tctgaagagc agcaaaaacc ccctgaagca tttccttaat
       61 cctccggaag atcatgctct cccactaaat atggtacctg tggacaaaca atggattttt
      121 ccataataat aagttgaaga tttcattatg agtgactaat ggatggacgg gtctaggtct
      181 gagcagatac acggagaagc ttccttttga tcagcagcat agggttaatc catggtaaga
      241 aataatggag gtttagagag aggtggggga gcagtattga gctggggtga atttacagcc
      301 gagagggctc agtaaaagga tgatttttag agcctctcgc cgcgtattga agggaagact
      361 gtctgacaag tcatcaatga gccccggcgc tgtactaaac taatgaaagc tgcatttgat
      421 cttcttcgaa aaggttcaat accactcctg ctattattta agtcctcagc ttcaccaaaa
      481 cctgttttca gatccacaaa aatcattaaa ttgcacagtg aagtaaactg tgtttgtaag
      541 tattagatga ggagggaata tctaaactgc ttataatgta tccctgttga ttgcatccaa
      601 ttgacacttc ctgactttgt tagtgtctgt ctaagtctct ctgctggttg cggttcagtt
      661 aacggcaaca tctatgcact gtggtgtaag atttatgtta tttattagca ctcacaaatg
      721 ttctagatgt tcacagacag atttttcatg cacttcgact ttttctgtct tttgttattg
      781 ctttgtttgg ttatcttgtg tggtctgatc attttagtcc tcacagtgac acttcatctc
      841 taatgggctt gaatgtgagc tagtccgtag aacagcactt gaagcatttt atgtgttttg
      901 accttgtcta tatttaacaa ttctggtccc aaaggaggcc atttgaaagt caaactgttg
      961 taaccaaata ccaaatctta tctcctgctc atagatttaa ctaaaacact gaggtctgct
     1021 ttcagctaca ttattttctc attgaaccaa aataaaaa
//
LOCUS       KCVB01000383            1595 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17735pri ultra-conserved element locus
            uce-406, sequence.
ACCESSION   KCVB01000383
VERSION     KCVB01000383.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17735pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1595
                     /note="ultra-conserved element locus uce-406"
BASE COUNT          446 a          343 c          315 g          491 t
ORIGIN      
        1 acctgcttgt ctctcagctc ggtctgatgt tgttgtcttg gcttctgttt cttgtcttct
       61 tcatctctgt atttaccttt tccgtcgtgc tgtattaccg gacatggaag ataattgcag
      121 agcatataat tatagagcat tataggagca atgggctttg tcttccacat taagtgaaac
      181 ttaataattc tcacctaaat cctggcagtg attaatggct gtgaagggaa ctgggcaaaa
      241 ataaaatgta catacaggtc aaatgctttt ttcagtaggt aaacaaagct ttgctgttct
      301 gaacaatatt ggatttaaaa gcgaactttt accaacatta catgggagaa agctataatg
      361 aggaaaggaa gtaatataga ttagatttat tatatttatt gtaattccac tgtcacttaa
      421 aagtttccat caaagaacat attagagaca aataggcagc gtacaccata tataagacca
      481 aagcagctcg caccacccca atggaccatc cactttttca tacatttagg gaaaatataa
      541 tcattctttt taattactgc acctaggcac tctattaaaa ttctaattca cctcatccac
      601 ctcgattgat ctgttcacag tgggtggctg tggtgaaatg cactgtataa tattcatgct
      661 ggtacgcctt ggttgtgctg tgtgtcagaa gccatcttgc agcgaatccc tcacccacct
      721 tggagcgcag cctgtggaac tgatgaggtg aagagcattg ctctgcagct ccatgctagt
      781 aatcacggga ctgaagaaac acatacatca tctgccacac aggttggcat ggcgagtgaa
      841 aagccttcac atcacgtaag atgttggaca aatgatggcc atacgtgtgt ttgtctcctc
      901 acagatggct gtgcatcttc agtcaggaaa aagctgagcc tggcacttat tcccctcccc
      961 caatccactc ttcctgctcc gtcttcctct ctccctccat cagtcttttg tagcacgtcc
     1021 cttttttttg cctctcctct gcaggaggtt tgatattggc aacacagtta gtaacctcca
     1081 cggaaataac agtggaaaag atgacataaa ttatttgcca aatgtttgac acatgcaggg
     1141 cattgtcctt gctataatca tcagcactct gtcacctgaa ggtatgaacg gtgccactgc
     1201 tgctttgtgt ctctctgcta aacctcttgg gctgttcggg gctcatgctc tcttcagtaa
     1261 aaagtattcg aggcagcttt cctccaagca gcaaggagga atgaatcaca atcatctgtt
     1321 aactgtaggg cttctgcact ggcatttgca tttgtgcaaa aaacatgagt ttagtccagt
     1381 aattaaacaa taacagtaac tagaaaagct acccagttct catagttgta aagatgcaga
     1441 ctcacggcaa atgtgaggtg cttttgaagt taaactagtc agtaaaaaat gtttcctttc
     1501 gtgattttgt tacttaatat gtcatcacta atgaagggtt ttttaattac atccttggct
     1561 cagtgctagt gtgccttttt ttgaaaaata gtaac
//
LOCUS       KCVB01000384            1419 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17776pri ultra-conserved element locus
            uce-894, sequence.
ACCESSION   KCVB01000384
VERSION     KCVB01000384.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17776pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1419
                     /note="ultra-conserved element locus uce-894"
BASE COUNT          441 a          264 c          260 g          454 t
ORIGIN      
        1 gaatcgctgt aatgccatgt caacagtacc atacatttca ttacagaggt caccagtgct
       61 gcggtgttaa tgccatgcga aaaattttca cagcacaagt cttagtccct ccaacaagct
      121 gaaaacagga gataaacatt ttacttgttg gctgatatta tactgctgat gtgattacac
      181 ggacccattt gcagtggtta aatgcacatt tgctcagcca aaggtatcaa aaaaaaaggg
      241 tgttgaaaat ggaaaagttc acagcatcac tacagtgacc gcagaaaaat atattcaacg
      301 tgtcagtata attcagtgtg tcagtaaaac ctgatgctct aaacaggagc aaacctcccc
      361 ttttaaataa ttgatatttt ctgattaaat gtggcagttt ggagataatg aattctttat
      421 agtgtgcctt gagcaactca acagctttgc cctgcatgac agctttatgg ggcgtctttt
      481 tgcccaaatt ttgaaagttt aacctatgta gatcaatgtg cttccttaaa agagtttaat
      541 aaattaaagt gcagggggct ttttttcctc agcgagcaga aaaagctgat gagacaggtt
      601 tagtttaatc tcaggcaata gaagtcatta accattctcc caggaattaa aatatagctt
      661 gcccttttct tctctcactt atcattagca tcactttaat ctccccgcgt ttcacactgc
      721 tattttaatc attacttact gccttttgac aactgaagaa catggcttct ccccgctcag
      781 gcaatatcct gattttagct gacaaaatgt aggacatcat ttaaaaatat ctaaatttcc
      841 tcttcccttt ttattctcct aacttccact cttcagaccc ctgaatgcct ctctatttat
      901 atcaaagaaa gcacaaaaga cgagaggaga ttgagtggac gacattcaaa atgtcatttg
      961 agttagctgc aagggtaacg aaatgcataa tacagtttac ttgtgtctat catgttattt
     1021 taatggatgg tgaattgcac atattgctct atgtgggtca tgttgggtgt tgggggtata
     1081 tttgtctgcc cttgaatcaa tgaacattta caacagaaac cacatatctc cagagcagaa
     1141 tataaataaa gtattatggc cagtctctca atgacaatga cagatgtagg gcaagcaatg
     1201 tcttcaactt ttgtttcttt attgttactg gtaacagtaa aattgaacaa taggactgga
     1261 tctggagcat gattaattta gaaatgatga caaaaaaaag atggcctata gctgtttgta
     1321 tcatattttg aaatttgtta ttgtgtgaaa ttcgtagttt ggggatctca tttaattttt
     1381 ggacaaaata ataaacccct cttgacaact gttttgcat
//
LOCUS       KCVB01000385            1171 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17819pri ultra-conserved element locus
            uce-1018, sequence.
ACCESSION   KCVB01000385
VERSION     KCVB01000385.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17819pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1171
                     /note="ultra-conserved element locus uce-1018"
BASE COUNT          308 a          277 c          259 g          327 t
ORIGIN      
        1 gacacatgca aatacacaga cgctcaaaca tttatgcaca tgtatattca ttcagcagac
       61 aaatctactc taaaggtgtg tttgtgtgta tgtgtttctt tcgtaggcct ttcggtagtg
      121 ttcaacacat aaatcagtat tttaggaaag catacattta gaggcctgta gttaggatcc
      181 atccagtaga tcaatatcaa actactaagc cttaaggcca ccatcttttt tttcttttag
      241 tataacagaa aaaaaaatct tttttttttt gggaaactta atgtgccagt gtctatactc
      301 attactcttc atacctcaaa acaagaaaag gaaaatctag tgccatttta tttgtcattc
      361 tttttgaaag aagtttatct attagtactt atccatcgga gacatgcatt cgcatgtggt
      421 cattgaagtg ctccatttgg tcgaacttgg cagggcagac ggagcacacg taggtggtcc
      481 cttcttggca gctcgcttcc gcggctacac ccactcctgt tccaacccca gcccctactc
      541 cagcaccacc gctcactggc atggcgaggg ccaccactcc agctccaggc tcagggacag
      601 ccatagggat ggggatggat accgggcccg gggtacctgc gctccccgac aggcctgtga
      661 tggcattgcc tgtgctgtgc agggccatgt gcctctccaa cagggtcttg tgtgagaact
      721 tcttcttgca gatgtagcac tcataggact tctctccacg gtgcagccgc atgtggacat
      781 tgagagagct cttctgggtg aagcgcttgt tgcagatgct gcactggtag gcccgcaccc
      841 ctgtgtgtgt caccatgtgt ttgattaagt aatccttcag ggagaacgag cgccagcaaa
      901 tgctgcactg atgaggcttc tcacctggat gtacatgcaa gacaaaagca ttaggggaaa
      961 aaagaagaga cacattttga ggatttggtt tgcaaaatat acaaaaactt gtgcagcaac
     1021 attggccgcc tgaagccagc agtgttactt ttccagtaca ggaacacacc tcaggctaga
     1081 ataattcaaa cctgttttgg cattgttaag atgctgattt tactatgcgc tatattctcc
     1141 aactctgtga gaaatattac ctccccataa t
//
LOCUS       KCVB01000386            1460 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17858pri ultra-conserved element locus
            uce-393, sequence.
ACCESSION   KCVB01000386
VERSION     KCVB01000386.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17858pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1460
                     /note="ultra-conserved element locus uce-393"
BASE COUNT          414 a          320 c          292 g          434 t
ORIGIN      
        1 gctagacttg tcatcacctt aaaataaaat gttctgagag ccatccaaaa ataggcttat
       61 cacttacctt tcagtgatct tggtttagct tgcttgcggc gcgacatcct atagaaaacg
      121 ctgaagttgc tcagtttctg tctgataatt tgtgtaagac gggatagtct aaaggtatag
      181 cctctctaaa tttcactgac cccaatcgcg gctgcctttt taccattaca gaaaggtgca
      241 acttagccca aattgccgcg cggtctaaac actagtttac tgttattttg tgatgagtag
      301 gtggatggaa taaaatccaa ttagcccgat cggtataggc gacactattt agttgagctg
      361 ggtatcggga aaagtgtgag aatacagtaa tcccgtcatg cgcttccatt ctccgggaaa
      421 acacacacgc gtgcaggcat gatgttcgtt taggaataaa agcggaatat ttgcttttcg
      481 cctctcatct caaatgcatc cgcgtgagaa agacatcagc aaaacgcctc ctgtgagagt
      541 gcaaaaaaat gcaacaaaac caaaaaaagg aggaaaaatg ataataacac ttttcacttc
      601 atgtccacct aacagcatca aaactattat agactcttcc ttttctcact ccagtcagct
      661 aatcatgaat taggttcgga atctgatcta agtctacgtc taaattgccc ttaaaagagg
      721 atgaagaagc agagagaaga tggaagctcc ctcctcatca caaacctgca aggtcttgga
      781 ctgcgctgtc gctccggtag tccacataat aatggaaaat ggaagcaagg cccccaaagc
      841 catcaggatg gctccagagg gggcccctac cccgcaggga ctcgctctgt acgtaatcac
      901 tgaggaaatc attgtcagcc tgcctgcact cacacacaga cagagaggca tgattaaaat
      961 cctagggatc atggcaagat gcggaatgct ggatctgctg gtccccggat ccggagtcga
     1021 actctaaacc caaagtcggc ttgctcaccc tggcgtctcc tctgcctcag ctcgccgtcg
     1081 gccactctcc gtctacggca gacggactgg tcccccttct ggtagaaatg gttaagcaga
     1141 gagacatttt ctgtgtggct gttgacatat atgacatgta atggcacgca tagaatttgg
     1201 gcatgatcgg tttatttata gatggagatt ttttttctaa tttttattgt tcactgtaaa
     1261 taacattatt ttaccatcac cccgaattat ttgaatatta ataataatat aaagtaatat
     1321 ctcttttttc aagtttaata taatatattg atttttttct gagttaaaca atttagcctc
     1381 ctccttttaa tgttatttta ggatttgttt tcttgtcttt ctctcttttt ttattgttta
     1441 atttctatac taattatttc
//
LOCUS       KCVB01000387            1229 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17894pri ultra-conserved element locus
            uce-707, sequence.
ACCESSION   KCVB01000387
VERSION     KCVB01000387.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17894pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1229
                     /note="ultra-conserved element locus uce-707"
BASE COUNT          362 a          234 c          283 g          350 t
ORIGIN      
        1 atttcctcca ggactacagc ttgtcactga cattaatgga ttcacaaacg cagcctcaag
       61 ctaatttgac taaaaagtca tattaaaact aattggactt aaaggtcaaa atagctaaat
      121 agactaggtg gatttattta attgtctacc aaaacatttt ccatttgcag tttaatctga
      181 aaaacacaac aaaaaaacga tttcctaaag atttttttaa ccaataaact aatattcatc
      241 tcttcatatt tcctctaaag gttatttatt cttttaaata tactaaaatg tctgttgctc
      301 ttggctgagg ctcacataaa aaaacatgtc aaaacttcca ctcaaaggaa agtaaataaa
      361 aaaaaacacc ttctgggggg aatttggaag ttcctttcaa atattccata acgtcccagg
      421 tttgtttgcc ctgctgtata tctgctttta caagccgtgc gtaaaagttg ccccgtacct
      481 gcaatgtccc acagctgtag acgcacagtc tcctggtccc agttgaggac tttaagggcg
      541 aagtccaccc cgatggtggc tcggtagttt gggctgaagt tgtgatggac ataccgcttg
      601 atgatactgg tcttgccgac gccgaggtcc cctatcacaa ggatcttata gagatgctcc
      661 ttttggtggt tgttctgcat ggcgagcgag gagggcgaga gctgagcatg aatccaccgt
      721 aatggagagg accgagggat gaaaggggag aagcacagga cagcgcagag ggaactgagg
      781 agagctggga ctgagggaag gggaggtgag gtccgccctg gcccacagag ggaagcagag
      841 cggagacgtc tggaccttgt gacttacgca ggggagccgc ctgggagtgt gtgccgggat
      901 gtgtttaaaa cttcaaaaat ggagaagaaa cgctgcttta atttaaagtt tgtattttgc
      961 tatttatgta tctctctttt tctttctctc cagagagtag agtagaagta gcattacatg
     1021 gaaatactca ggtacttcac aacagtactt aatggtttct attagtaggc taaagcagaa
     1081 atagaataaa atattcatag gcgtgttttc attggtgtat aatctaatga aatatgaata
     1141 attgtgtatt cagtttctta gaatgaacct tttatttcta cagagggtgc gggtcctctt
     1201 ccacggagtc tgccatgttg ctacagtag
//
LOCUS       KCVB01000388            1173 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17931pri ultra-conserved element locus
            uce-869, sequence.
ACCESSION   KCVB01000388
VERSION     KCVB01000388.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17931pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1173
                     /note="ultra-conserved element locus uce-869"
BASE COUNT          318 a          222 c          272 g          361 t
ORIGIN      
        1 aagtctttac gtttctcagg cagactagag gggtatgtgt gtggtgtaat ccacagttaa
       61 atgtctttat aaaccaagta tgggaattgt ctgtaaaatg ttcaattgct gttttaggct
      121 agggatgctc cctgcctgac tcaccctcca ccacaacccc cctattttat atgtgaactg
      181 tctggtgcgg ggagggcata tatccgtgtt gggaagtgag tgaccacctc ttcaggaaca
      241 gtccggactc cttcaggccc ttggacggtg aggggatacg aggggagaga gctgaaacac
      301 aagtgtcacc tgaccatact gtcttttcac tttaggttat gtgatatgta gctggcacag
      361 tgcaagagta caaaaacagt tttgttttgg agtgactggc tttctgtaaa gtctgagtct
      421 tgtggtgagt acagcatttg tcagccttcc cccccaccat tgttcctgca tgcctgcagt
      481 gagagtgagg gtggggggca tgaggaagtc ttgacttttt tttgatggga aggggggtta
      541 ttttgattgt ctttacctat caggtgtaaa tttccaggca ctcagttcat tttgggcctg
      601 ctccagtttg tctttagtct gttctagttc agccttcatt ttgaggaaaa acaagttgat
      661 ggctgggtcc accatggatg accgcagttg ggccgcactc ggctgctgga cttgcttgag
      721 gtactggatc tgggtctgca ggataaaagg aacaggtgtt aatgtctggt aaagtttagt
      781 atatttctac taatagaaga acttttgatt gctgtgagta ccagtttttt tagcttgacc
      841 aatctccagt cgatgaagtc agattagcca ttttctccaa aaatcaagta aattaaaata
      901 acgtggttgc aaaatccaac aagtttatca gatgactttt cagtcctgag cctaaggaga
      961 ataaactggt ccaaaataga agactaaaga tgacaacttg agtaatttct gcttgttgcc
     1021 tttcaactag aacatttaag cagcttagaa aagaaactag atttcaaacc aacaaacaaa
     1081 ccaagtactt ttattgtctg caaaataaaa gtacttgatc agaagattag ccataactat
     1141 taccattaat catatatact tgtatttgta ttc
//
LOCUS       KCVB01000389            1355 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 17976pri ultra-conserved element locus
            uce-1025, sequence.
ACCESSION   KCVB01000389
VERSION     KCVB01000389.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="17976pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1355
                     /note="ultra-conserved element locus uce-1025"
BASE COUNT          462 a          283 c          254 g          356 t
ORIGIN      
        1 caacaactat taaatttcag atccaaaact agtatttcaa aatcttatga cagaaattta
       61 aatgtgaata ctgtcatgct acaaaccaca tgcacagaaa aactctgagg atcagtgtaa
      121 cgtctcttac ttaacatgac acttaacagc ccaaatccac ctctaaaata acagacttgc
      181 cacagatgca ctatgataac tgatacattg actaatgttg tttcaattta atcacacgtt
      241 acgccaaact attaaagctg aagtgcatgc actgatcctg aacaaaaaaa taatctgcac
      301 aggcaactat ctaattaatt attccaattc caacgaacta ggtgatattt ctataaaagc
      361 catgggtaac atttcggtga attttttaac cctttatttt ttaatggtag aaacatatga
      421 tatcatatac catatgatgt accatgtgaa tggggttttg ctcttgaagc acaaacaaaa
      481 tgcatggggg ggagcagtca gagaagaaaa ctacattacc cacgggtccg cagattgata
      541 gacagatacc caaagcttaa gattaaacca caggagtcct caaggcgccc caggggccaa
      601 ggaccttcgg ccaacctgtc atccccgatc ccagtgcccc agggtggctc agaccacggg
      661 cgtctcccac aggagggggt ggggtggccc tgggggagct gagaccccct tgcatcatgg
      721 ttgtcatgcc gagtgcttca tcttacgtac cattccacat cacccaggct ttggtaaatg
      781 tagctggtcg ctgttagttg gttgcaaggg aaaaaagaag ccaagttaat accaaggcct
      841 agagtactga cattacagat ttctatgaag acaaaggcac acacacatta agcacttgtg
      901 ccttgtctta ctatatttaa aagctatcca gttttacatg gagtttgttg agatatcaat
      961 ttcagtgtaa tatcaaactc agatgttata tgtagactat tacacttctg aaactaccag
     1021 taatgcggaa actgaaaagt ttaaaaatgg tttagagtac atggataaac agaagggcac
     1081 tttgacagca aaaacaggtc aacatcatac aacataacat catattgaat aaaaccaaac
     1141 acaaatgcaa tgtgacatgt tgcatgtaac aaaaacaatg tttataattt ccaaaaagag
     1201 aaaatgatta aacgtctaca cttacttttt gcctctgatc tgcctcagat ggtggaaaac
     1261 attgatgaca tctcgtattc gtcgcggcgc ttcttcaatc ttggaggcca agttgataca
     1321 agccatagca acaatctgga aaaaatataa ttgaa
//
LOCUS       KCVB01000390            1678 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18009pri ultra-conserved element locus
            uce-1060, sequence.
ACCESSION   KCVB01000390
VERSION     KCVB01000390.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18009pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1678
                     /note="ultra-conserved element locus uce-1060"
BASE COUNT          477 a          383 c          349 g          469 t
ORIGIN      
        1 ctggtgcggt cgatcaggca gttcttctga cgggggcagg agtaggctgc attgctctgc
       61 tgactcctcc tgaagaagcc ctgcacagga aaaggggcac ggaaacactt caggttactg
      121 gaagaaccac tgtgggtgga caaaatattc tgggattgaa ggatcaatcc gtgccttcat
      181 agtgttttag tgctgacaga attcactgat tgactgtgat ttattggatt tctggtgatg
      241 atattttcat tttacttctg ccaaacagat tagataataa cactactgtg atggtatttt
      301 cctggctgtt gcgctgtaat atttggcatt caaagagcgc tgtattttca ccagtgtgtg
      361 cagcaggagg gtggcatgtt tgggtaggac tgataataac agtggatggt atttttcttc
      421 agcggggtta ttagtgttga ttaacgccgc actggcatgt gcagtgctaa aggattgcaa
      481 acgcacaccg actggattcc tcatcctttg acttaatgaa ttgattccag cacatcatta
      541 acatgcccca ctctactgct ccatacacat atgtcttaat tagctgctca ttggctgttt
      601 aattacaaga aaacagctga cagctgccaa gttgctttat aatggaagct attacggaaa
      661 caggctataa ccgccagtga caagcagcac tctttaataa tgctatgatt tatggtccaa
      721 acttttgtac tcgcacatat cattaaaggc ctctctgcct tggactaatt agcaatcatt
      781 aaagatagat ttcagcagtt tattctttag tgttttgttc cttgcagctc ctgtgtgagg
      841 gagaggagca ctgggaagaa cagctgaaag gagcctctct ctccctcctt ctctctcttt
      901 ctctctctct cttccacaca cacacacaca cacacacaca caaacacaca cacgcacagg
      961 agcaaacagc aaaggtgcag gctggctgag gaatgctaat gatgtgctgg tgacaagcac
     1021 agggacaaga atacactcta agcacaaaat tcccctcact gtgaatggct ggtgcatttg
     1081 aaatagtaca aaaggcggct tcaatggaag caacactgta gcaaacagcc aacaatctgc
     1141 tataattcaa agcactcttc ttttgatcat tttatttcaa gaagagcagg ctacagcaga
     1201 agcaaaaaat aaaagaaaaa aagaaaaaga aacagtgaaa agacatgtcc ctgggaggga
     1261 aaggcagagc aaggcagagg aatgactttg tcctactaat tgtggttatc tgtgatgtcc
     1321 tctgtagtgc ttgaccactt taatgatcat gttgtgtgtg tttactcttc gcactccctc
     1381 tccaaatcat cgtaaaactt cagcaacatt ttcaaacgct ttttcctagc aagcaacttt
     1441 tctttacgtc ctcaattttc cccgtcttcc cctcctcagc tctcctgtcc ctcacacaca
     1501 cacacacacg ctcacactca aacttcttcc catgacacaa acagattcgg tctcttgatg
     1561 cttaccttac agccttcaca tgttatcacg ccgtaatgga tgcctgatga tttgtctcca
     1621 cagatcttgc agggaattat ttcgatttga gctgcagaga agaaaaggaa atgaaaga
//
LOCUS       KCVB01000391            1021 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18051pri ultra-conserved element locus
            uce-483, sequence.
ACCESSION   KCVB01000391
VERSION     KCVB01000391.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18051pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1021
                     /note="ultra-conserved element locus uce-483"
BASE COUNT          316 a          215 c          222 g          268 t
ORIGIN      
        1 aaaaaaaaac tccatgcaag agacatgaca aataaagtca agagaaaata taaagagacg
       61 tactttttat aaggttgtgt cagtatagaa ccagtaggct tatatgagta aacttaactg
      121 aatatggact gaatctattc tcgatgttct ttaatattag gcctacattt aaatttacta
      181 cagttaatca taccatgctt agaagacaga tgctggggaa gtatttcggt tttttcagta
      241 caacccacat cattgcgcgg acaatgaaga tagactagtt tcataatctg ttaccaaaac
      301 atcacaatcg tttcaatctg ataaaaatga ccgcaggtct gccgtgtggc acacagagtg
      361 actcatctct gccgggactc gaacccggag cctctggatt agaagtccag cgcgctattc
      421 cattgcgcca cagagactgc gccaggatgt agtgggttta gcacggttta catacttcat
      481 attctcccgt atcgttgctc tctgcatccc tgcagcatca ggaccagtct gagccgacat
      541 gttggttcta ccgctgtcga ccaccagagg gcgctgttgc ttcacgctga ggcgggcagt
      601 cgctcggatg tccgtcaagc tagctagcgt aaaacaggaa gtgtgagtat aagtacaaaa
      661 aaataaaagt cactgttact gtagacggcc tgtcaatatg tggtagtatc gaggctttta
      721 ttttgaaagt gcaccaccag gaatctttcc tcatttaaac taactacacg gacgtcgcac
      781 cagagccaaa atggtgcctg tcgcgtctgt cggagctttg tataccatgt ttaaagacaa
      841 cataatagcg aaatgaggca ggttccactt agccagcgac gccaaaatac gacggctcac
      901 caactacaac caatttaaaa agggggaaaa agagtgtttt tgatttacag cggaaaaaca
      961 gagaatgggt ctagatattc cttttagaaa tagactagtg aaaacatcag gttttattta
     1021 t
//
LOCUS       KCVB01000392            1475 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18096pri ultra-conserved element locus
            uce-56, sequence.
ACCESSION   KCVB01000392
VERSION     KCVB01000392.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18096pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1475
                     /note="ultra-conserved element locus uce-56"
BASE COUNT          418 a          389 c          270 g          398 t
ORIGIN      
        1 gcacttaatg acacacagct catactggaa ggtggtgaac tcttaatgaa aactatgtgg
       61 tgtgtaaaga aagtgaacta aagatcggcc caataaaagg tgccgctaat aagtttttct
      121 tacgtctgat acgtggcatt ctctagatgt actatatgat tgaaaggaca atttggcaca
      181 tgaccacttt cctcaacaca gaacaagtga ccttgtgagc cgccaaatgg tgcctgtttc
      241 aggttttcaa tgtggcaggt gccttgatca tgattcaacc aacttgtcaa caagcgctgt
      301 tttcctgaaa cttggcaggg actccacaac agattccaag ttgcaacttg gatgatcaca
      361 ttccaaacct gcaggagttt tatctgtgat ttaccccttt attgttatgc tgacctgcac
      421 tttaaaaaac acactttacc aatgcaggag ggaaaacatc agagcagact tcaggtatcc
      481 tgttagacag taatttgatc cagagcgata agatctgcaa atgatagaga ccatgtattc
      541 atcctgtctt ttttgatgga caggctctgc ccaaccctcc tacacactga caagtaggaa
      601 atatgtttca gggaatgaca ctagcatacc ctcttacact taatcattgt caatcactct
      661 gcgcacttgg ctcgttgggt aataggaaca gatccagcat cgcatcccct ccaatctgtt
      721 tggctctcac acgggaggtg catgctgttt tcatgcctgt gtgggatggc ttcaagctgt
      781 tttcttatca aaaggcccat tcagacattc tctttgagag gcatcgcaaa cctcagatct
      841 cacagcctcc gttcaaccac atgtcttgat ttaagaggcc agaatcagat ttgctgccac
      901 gaagagctgt cactgctaaa gaatgggatg tgatttatca tcctgaacca cacacaagac
      961 tgataaccag agcgctagca tttgtacgca tatgtgcaca cacatgcatg tgagggcgca
     1021 catatacaca cacacacgcc aactccccac cccttcactc ttccccctca cataaacacc
     1081 caaaaaaaca gatcaacaca tgttctcaag ctcactcatc ttctgactca atcacttcta
     1141 tatgccttca cacgaaaacc gcaatttcac atagaagctg tcttgcccct cgctctcttg
     1201 cagtgtggag ttggatcaca tgctttttac aaactgcaac tacatcacct tccacccagg
     1261 gatcctctct aaacgctccc ttttaaacct ccactaacag tctcatcatt gattttatgt
     1321 ctctgttttt ttttctcctc gggttgtaag caagcaaaca cagctgaacc ctaacaccct
     1381 gtgtcccgct gtgtctcctt cagggtgttc tcatcacaaa acagctacac aacatctcag
     1441 agtgaaacac cctcagcaac acaggccata gaaag
//
LOCUS       KCVB01000393            1294 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18134pri ultra-conserved element locus
            uce-811, sequence.
ACCESSION   KCVB01000393
VERSION     KCVB01000393.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18134pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1294
                     /note="ultra-conserved element locus uce-811"
BASE COUNT          394 a          224 c          301 g          375 t
ORIGIN      
        1 aggctttaaa ttgggaacaa gaatgtgttc ggtcagcctg aacagcgcag gaggattttg
       61 gggaggactc ctcctttgtg acacatgcag catagacata atgaattata ttataattac
      121 aaagcacagt ctgtagaagt ggacaatata gatatttggt caggggggcg gagtgtgctg
      181 ctccatagag acagggccac tccagactgc atttctattc agatcacttt aacttagaca
      241 aatctcaata tgaatgaatg gggctttgtt cagaaaataa aaacatttga acaaggaggc
      301 atcatcgtct catacgctgg ggcaacataa gcaagtgtac acgtctggaa aagacactga
      361 gctgccaaag tttccccctt tttttttttt ggactttgtc aaaaagtagt catccaatat
      421 aacagacaaa atatatgtta ctcatttaat aaataaattt gtgtaggcag ggatatgtca
      481 gagaacattg gtttgtctgg aggagtgtgc tgtgtgtgga ggtatactgt ggagagattg
      541 ggcaaagcag agcgagcaga gcgagcagag gggagtgaag ctggcagtga ctgagaagat
      601 gaggatttga cggcaaggtg acaaagagtt caaacaaaat gaggcttgta gattagcagc
      661 actcccctca gtgacaggtg tcatctcttg gggcagtctg tcactctgcc tgctgccact
      721 ctttatataa tatgaggaga gagagagacg gagagagagg aggagaggct gcaccacttg
      781 cacctcacga ggcacggcct agcctgctct acctgccagt aactgagatt aaagtctgca
      841 gtaagtgcag agggagagct ggacggacaa aagaggagca aatgtttatg ttccttacaa
      901 gcgttttttt ttttttttat gatttcatca taaccagcaa cttctcaatc agagtcaatg
      961 taaaatcact tagtcaggga cattatcttc taatagagaa acactaatgt ccataattac
     1021 tctttgacat tgcagtttca gggtttgctg ttcaggcaat ttaaactgct ttacatcttt
     1081 tatctgtgtc atattagtca ttcactttct tcttccagtt cctggatgta ttaggccatt
     1141 gtaggccttt ggaaatcaaa gggactgttt tgtggcagta ttggattttt atataatttt
     1201 tgcattaatg tgacaaaatg cattcatttc aattagctaa aaaacaaaat aacatgaata
     1261 ttttatccat aaacagtttt tcagtgaaaa ttaa
//
LOCUS       KCVB01000394            1573 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18238pri ultra-conserved element locus
            uce-1324, sequence.
ACCESSION   KCVB01000394
VERSION     KCVB01000394.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18238pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1573
                     /note="ultra-conserved element locus uce-1324"
BASE COUNT          460 a          288 c          297 g          528 t
ORIGIN      
        1 actaggccta ataggtggat ggtagaaaat aaatatcatt ttgtgattat gttatcattt
       61 ttattaaaat attcagattg tttactatac atattattac tccaaaagat acagatgact
      121 ttccatttta atgactttcc tgtcagcaag acttgtcctg aaagacattt catgctccct
      181 agatgctaac tgatatgtgc tggtctacta taaaaagctc aacaagtcag ccacactaag
      241 ctctcactgg ctgctggctg taaacatata gactcttaat ttgatggcca tggaaaatag
      301 tttcttctaa atattgcttt taggccttta cattgtgtct cgtgtttcga ggcatggaaa
      361 tcatgaaata aagacagagc aaatgcaaat gtataatgga aatatttatt tctaaaatac
      421 aatttaacat actttaaact tatgactgtt tttggacaac tttatcattt caacatcaga
      481 gagaaaaata aaaaaacaac attatgtaca tggtcttgat atttacaggt ggtgtgaaag
      541 ctggacttgg ccatggctcc gctgctgtcc cgcttgaggt gcaaccaaat cccactctaa
      601 aatgtgctgc agtaaacgtg tcctgatttg gagattgtct ttgaacatgt aaaagtcgct
      661 gacttgtcct cctgcgtttt gtcctttgtt attttgttag tctgtccagg ctagtagtcc
      721 tgatgtgggc tgtgcgtcaa actgtgccgt cgcaagtcac agttgcgccg aaacaccttt
      781 ccgcagcggc cacagctgta gggcttgatg tctgtatggg tgagaaggtg agttttcagg
      841 ttacttcttt gattaaaggt tcttccgcat gtggggcatt tgtggggaga ctcctgttta
      901 gatttaaagc aagcaaagga ttattccctt taattgtata gaatgtctat caattgtatc
      961 tttcagtcat ctaaactcca ctattgtgtc agttataact cacaccggtt tacacaatat
     1021 tttttatgtg tatctctcac ttacattttt gttttaattt ggtaattggc caaacgcaat
     1081 cttttttgtg agactcagta ttatccccat gcatgcagca gaccgtgcat aataggtgaa
     1141 ttatgatcat aaagtgccac atagttaagt gagaatgaaa ctgattctat tttcagctta
     1201 cctgcatatg taaggtttta tggacggcta gagttcgaga ctgacagaat cccttcccac
     1261 attcttgaca tttgaatggt ttttccttgg agtggatgta tctgtaaaac agaaataaca
     1321 cttaatcaaa agtccgctca tgcatgaatt tgcttgtagc tgtgacaaac cccagagtca
     1381 tagggtgaaa aagctccact gtgcaagtta taagcgtcca attttatgca aaaaagtgct
     1441 ttagcgccct catggagaaa actctaggaa caggtctgcc tatatgatac agcaaaattt
     1501 aaaaattata tattttttat tgcaattcct caagaaatct ttcagcgaca tatatacata
     1561 tttattttta ttt
//
LOCUS       KCVB01000395            1706 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18281pri ultra-conserved element locus
            uce-353, sequence.
ACCESSION   KCVB01000395
VERSION     KCVB01000395.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18281pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1706
                     /note="ultra-conserved element locus uce-353"
BASE COUNT          435 a          365 c          416 g          490 t
ORIGIN      
        1 gtttgagaat aattggtatt aaattgctgt tactggccag gacagataag ccatcatgcc
       61 tgtaaaagat gagaaagaca actcatttag aattataaac ccttttctct gtttgtcact
      121 tctattgatt ttcttctgcc cccgtctctc atagtttata tctagctata agatatactc
      181 ctgaagtgga tttattgcag ctataggcct tgcccgccag gtctgccatt accaacagat
      241 gaaaggcggg ggatgggggt ggtaggggca ctacagcagt gcagtatttg atattgttta
      301 aatactgtaa aagagccttc tcatttgcgc agcgagagcc ttgagaagca gaccgatgtg
      361 ataatcctgt gagtagcagg tggtgctgct ttaatgattg tgtaaaacaa aattgcactt
      421 ttccattttc agtccgctct ttataatctc taaatgagac gattgatcgc ctgttggcaa
      481 gaaaatggtt tcaccggttc ttattcaaag tggacctgag gagaggatga tggtacactg
      541 acaagaaagg aaaaaaagag gcacattgtc tttttttact ctctatctcc tatctttttt
      601 ttttccctcc ctctcagact gggctgtcag gtgtgaggaa gaggaggagg aggaagcagg
      661 ccttttttct tcttccccca ccacttccga attacctgct gggtccgagc tcttttatgc
      721 tcttgttgtg acaggtagat ttataaggct tgaaatttgt acaaatcagt tgtcattcag
      781 cggcagctgt caatgtgtca agctgtcagg gcctgctgtg agggagatgc cttagggggc
      841 tgagcgaggg ggcaaccgcc cagaaaagaa aatgagttca aacagacctg agaagctcct
      901 ctattccaca ggcctccctg cccctgtgga gacgccatcg atttctcttg atgttgagca
      961 acagcgcgcc caaaaatgac acgccacacc gcgctcaacc cagtcaggct gcgacacgcc
     1021 aacgcgtgct gtgccaatcc cctcaatctg cagatgcctc tcattctcta ctaaatggct
     1081 ggaaatctgt aatctctcca aggagggaga acaccagagt tgtgtttagg gattgttggc
     1141 gatggctgtc gtgtttttat gtgccgctta agtagctgaa atggaaatct ctcagaggag
     1201 aatgacaagc gtgtttaaag attgttgtcg attgttgtct tgtttgcttg ttttctctgg
     1261 ccttgtctca gcgtgggcct gcctgtgtgt gattggcggg cctgctgggg cagtgatggg
     1321 catgacagcc tctctcagtc agaggacccc aggtgacaca caccttctct atcctctatc
     1381 cttctacaca gtcttcacag aggaatggga aagacaggtg ctgcaaccag tgtgaggtgt
     1441 gtggaaacaa aagccaacag ctgacagaga agctgtgcca ttcgacagtg aacatgccaa
     1501 ggtgtgggat gacacaaata tgctcatatt tctctcatac acacgtcttt gtggtattat
     1561 tggtgttgtt gtcacattca gtctgcggga gcagatggag atgaacacgt actatagata
     1621 acacataatt aacacactgt cgcaagtctg tgatttgaag tcaataatca ttattgtgga
     1681 tttttagtag ctgtataaaa aatatt
//
LOCUS       KCVB01000396            1740 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18323pri ultra-conserved element locus
            uce-316, sequence.
ACCESSION   KCVB01000396
VERSION     KCVB01000396.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18323pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1740
                     /note="ultra-conserved element locus uce-316"
BASE COUNT          474 a          351 c          353 g          562 t
ORIGIN      
        1 ttttcattaa gccaacttaa taaactcagc aagagtttca ccaattacat taatctctaa
       61 ttcctaataa aaatattacc tatggaggag gatcaacata gccttaaggc aacacagaac
      121 caataggtta agattttaat tgcctatttt gtttacctat atcagaattg agtaactaat
      181 tatttgtggc gctttttccc tgttactctg atgtatttat ggttctgtta tctactattg
      241 ctgttatttg tttgtttgtt tgtttttttc ttgtcattac tattgtcaat gacgacgttc
      301 tccctctcgt tgataaaatg caatgatgaa aaaaaaattt aaaaataatt acctatggcc
      361 cgtgtctatc acactctttg catgctgttt aggaagacga acgcacagga tgttataacc
      421 tcacatcatt aagcttataa ttaagcttta gtctcctctt tgttcattcg ctgtatccgc
      481 tctcccagta tttatcctct atcagattgc tctgccctgc tggagagcag agagacagag
      541 ggctcataga aaacctcttc acctgctccc ttcacccctc ctcctcctct cctccatgtg
      601 tacctgcaac actgggctgc agtgcatcat gggtgcagct gaggccatgc caaaacccaa
      661 tttggatcgt gtccagcact tcattggaaa gtaatggttt tcactgaact gagttatgac
      721 cagctgtttg cagacagtct gtgacatact gatggagcag aacaccgtat gttgctttcc
      781 aaatcaaatg atacaaggag tgtgacagtg tggagaaaca tctgtcatta gcaattttca
      841 gcacgctcca ctaattctgg acaaaatacg cctaatgaag ttactgcagg aaacttcaaa
      901 tattgtcagt gcaacaaggc cataagaagc cggtgctcac caaggcctgt aatctgattc
      961 attggttgtg ggaaaaatac agaaggagga gggtaattgt ttttgatcat gatgttttaa
     1021 aatgcaaatc gcaaacgttc gtctttaatc caagaacaaa aactcgagaa atcaagggcg
     1081 agagggtggc tggcttttct ttattgatgt cccctccctt gttgtggggt ttcatagatg
     1141 gagtttccat atggagggac gcaaaagctt tgatgggcct ggttatcagg ttcagttgtg
     1201 gatgtatgct acgtgacact gacaaagcga tctcatctga aacagcgaac cctcagagga
     1261 tcaggtggct ttcagattat ctcacaggtt gtctcttcag tgaattcaaa atttctaaat
     1321 aaaacttgtt gaaatgggaa ctagttttat atatatttat ttataaatat ttatttatat
     1381 ttgttttata ttttttttcc cccgatatgc tttggcgctg tgacgaaaca ctagatagga
     1441 ttgcctcctc attgccttta tgtttcaata ttgagttgaa atgaagccta taagtctagc
     1501 actaacaata ttcatttggc tactctgtgt ttgttatttg ccagtttcca agaactgtcc
     1561 tcaatttatt cagactctgc tgtgaggatg agagggtttg tgacaggcag ctctgctgaa
     1621 agtggtcctc tcctccagaa cagttgttgt agtgagaaat gttagagacg ggtgggcgtc
     1681 tggacccaag tatgctgatc aaacacgagg cctgtgtcta tctggcagcc atctcctggc
//
LOCUS       KCVB01000397            1629 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18366pri ultra-conserved element locus
            uce-526, sequence.
ACCESSION   KCVB01000397
VERSION     KCVB01000397.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18366pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1629
                     /note="ultra-conserved element locus uce-526"
BASE COUNT          505 a          333 c          330 g          461 t
ORIGIN      
        1 aacaacaata tcataatctg gaaaaattat gtttcatgtg cactaattat tgtaccataa
       61 ggacacaaac ctgttattgc atcatttaac atgaagtcca gcctgagaca gtgaatagct
      121 tgaaaattac ttgtagctgt agcaaccaag aacaaggtca tttccttaaa ggtccaccag
      181 agggtgcaat atggtctaat gtgttcatag cctttgttac tggggtcatg ttgactgcac
      241 aaaagagagg agaaacagag accttgcaca acaagattcg ctaacagatg aaaaggatat
      301 tttctcttct tgatatagag ctaaataaat ataccataga taaaatactt gctgtgatac
      361 tgcacctgct tttggtctaa acctttcaat ctgcagaaag agctttgaca gcaaaactat
      421 caaataaggg atctttaatc tcacactgct gatgttgtat ttctgttaaa gcaattgtaa
      481 aaattaagaa tgtattgtag gcccaagtgt aacatagtca cttacaggtc aaagctaacc
      541 ctatgataaa ataataagat ttacaatata ttaattgcaa gacggcacat atcaaagtgg
      601 ccgttaacta cctgaggtca atgtcatact gtctaattaa ccaggcagcg gctgtgtggt
      661 cctacatggc tgtggaaggt gcagagagca ggcgatctca cgcacccccc accaccctgt
      721 acccaccttt tacttcctcc cattcagagg cacgcacgca cttgctgtct gagttgcata
      781 gaacactgag acattccatt tgcatgcccg ccccatcaag ctggatgcac tcagactggt
      841 aataaaaggc attgaccccc ttaaccttta tgaattacaa tttgtgcctg cagcacgtcc
      901 tgtcagaggc ctcagaaaag caggcacctc agccagggtg tgtgcgtgtt gttttgcagt
      961 tgttaaggag aggggaaaag gtttttacag ctcctcgtcg gagctcctga caagcaggga
     1021 ggaggcggtc tgacaggaga agcagggtag ggagggactg cagcgctgct gatggctcca
     1081 tgctaatctc catttctgga tgctgcacga aactactgag gctcctttct ttgtcaagac
     1141 caaggataca cagcctgtgg ggctgcagtt catctgcgtt ctacatctga tttcttactc
     1201 tgccgtacat cacttaaggc agttccctct ccctaaagta ttaactgaag ccgctaatac
     1261 ataaatcaga ttagtctttt atgaaggttt tctgtattga atctaatttg tggggacaga
     1321 aagaattcag gaaacaagtc aaactacaac acttgttgac tgttttggta aaataaacga
     1381 gaagaactga actacaaaaa aaatctatca tttatactat gttactatat ttttgttata
     1441 ttttttgtgg agttgtgtac taacattatc caaaatgttt ccaacaatgt tcaatctcag
     1501 agaaatctat aattttattc aaggtacggt gcatttcatt ttgtctcccg gtaacaccag
     1561 tcagagagtg aggaaggaag tctgcaaatg ttattaaaag caacatgaat aaaactttaa
     1621 tacattaac
//
LOCUS       KCVB01000398            1329 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18438pri ultra-conserved element locus
            uce-1239, sequence.
ACCESSION   KCVB01000398
VERSION     KCVB01000398.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18438pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1329
                     /note="ultra-conserved element locus uce-1239"
BASE COUNT          391 a          246 c          253 g          439 t
ORIGIN      
        1 tttgctccct ctgtaaatat gcatggctca ttttgagtta ataaaaacac attgattcta
       61 attttcaggt gtttatacaa taatgaaaac atacttatgt tataatagtc cattactgcc
      121 aatagatccc actgaatctt acatactggg cctttaatta cagcagtttt caaacgaaaa
      181 tgagtttttt ctttaaaatt agttttaaaa tcataaaaaa ttgtgattct aaaatttcta
      241 attactctaa gcatatgtgt aatatgatgt aacttctaaa atatttgctg tagtgtgtga
      301 ttccatatca tccaatattt taataaatat ctgatatttt atttaaaggg tattaacaaa
      361 ataatatact agtaatttca tgctctcatt atgaggcatc tactgtatca attgttacag
      421 cgcagccttg tctgacctta ggataggaaa tgtacagtca aacggtgccg agcatgacca
      481 atcatgtacg tgtgtgtgtg tgtgtctgtg tctgtgtctg tgtgcacttg tacgtttagc
      541 aagacagtgc cttggggtgt aacggcatcc tcctgccccg actgtcagtt ggttagctgt
      601 ctacacaatg tcccgtttga ccttgtgttg gctacaacac tggctgaatg cacatctgac
      661 acactccctt cctttgtggt gcaatcctgt ggaagtcatt tcatttactg ggctttcaac
      721 ttcagccagt gtgggggttg ggggagtaag tctgtgttta atcagcctgc cacaaaccat
      781 tcccctaaac aaaatcagct atgatgtcca gcgaaaagat ctctcaacag aagattgaat
      841 taaacatata aatgggcttg tcacgttttc atgttttatt tactctcttg gcacagcttc
      901 gcccgccact ccatattatt gctggaaata acaactgtgt gttaatgtgt gattgttcgg
      961 cacaatatgc cagcattacc agtgtgttag tgtagtgaat gtataagtgt tatccattta
     1021 cacttaagaa attacagctg agaaataact gacattaaga aaacaaactg caaggaaagt
     1081 aaaagaagaa gaaaacataa ttaattgtct cgtgagcttc tcatcactct tctttcgtag
     1141 gaatgtgagc atttctggct cagcctctgt gtcaacctct aaaaataagt ctatccatga
     1201 ctaagtaact gagcagttga gtgataaagt tgagccactg gttggatggc cgagtgttgg
     1261 cctgttttct attacatcat caacaggggg aaaacacaag cttatgaaac ttatggtcat
     1321 tgtttagtt
//
LOCUS       KCVB01000399            1562 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18563pri ultra-conserved element locus
            uce-1138, sequence.
ACCESSION   KCVB01000399
VERSION     KCVB01000399.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18563pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1562
                     /note="ultra-conserved element locus uce-1138"
BASE COUNT          481 a          311 c          301 g          469 t
ORIGIN      
        1 cctgcagcgg ctgcagctct gaactagagc acacctagga tttcactacc ttggaggaat
       61 tttggtgttt tatgtttggc atgtctttta ttgaatagat gaaatatgag agatacttgt
      121 aactcctatt gacatattgg acatgccaca agaaaaacaa aatataggtg tcaaaaatcc
      181 tctgaggtgt caacaaataa atggtgcatt tttgttttat cttttcttgg catgaaaata
      241 atgtcattcg cttttgaaag tggtgtcagc tgttggaaat atctcaccat tacaagaggg
      301 atgtaattcc cttacctgtt gaaaagtggg taaaagctta caccatgaga aaaaggatcc
      361 gagatggaat tttgttgaag ttaaaaaaag aacaggtgtt tttactcttt aatttcatta
      421 gtgatgcata gggctttctg agtccttcta gccatttcaa atgaagaggt aaaagccaag
      481 cataagctta aatgataaca ttctttctca gagaaaaata tgcatgtacg aatcagagct
      541 attgtactat ccttattatt atctgcaagc caagggctct gttccaatta aagaatacca
      601 cagatggacc attcaacaag catgcacaca aacgaaccta caataggtac agtaaagtac
      661 ccagtattac agagcccaga gaagagcttt tactttaaaa actatttaat caggaaaatc
      721 aacgcctaac atcaaagaaa tggagatgag ggacaggcgg ataaagaggc tcatcaaaga
      781 aggagatcta gttagggatt caacgcccta attccctttc tcttaaaacc ttcgtcagag
      841 ggggcacagg gccaatttgc tccctaaatg gctgcgtgtg aatgtgcaga ttgctttgca
      901 tagccgctgg gaatctgggc ttctttctct ttttcttctc cacgcggagc ctgtgaatta
      961 gaaaagatcc aaagacagat aggcttaaag gagccctagc taccttcctt aagtccagag
     1021 ccctgttcca acttccaact aattcaatcc atggcctaac tcaattaata gactttaatg
     1081 cactgtttct tctttatctt tttctctttc ctgaggattg gagactgaag tagagttgta
     1141 tagtaatttc tgcaaaaagg tttggactgt aaggctgtct gaatgataag acaatgacat
     1201 gtccctgtgg cattacggtt gatcatatat gcatccttaa tctcaaacat ctccttaatc
     1261 tcgctttctg ggacttgcag tacattaatg taatgtactg cataaaatat tgacaaacat
     1321 tctctcctgt ctcagactca gggattcatt ctcagattca ttagtcagaa tgaccttgat
     1381 gtgagagtgg accctcaagt gtgtcaaaac caatcatcat ctgcgattgc aacccccagc
     1441 tcaaggtgta cattttcttt taatccaacg aggggctatt tcagtacttt tacatgagct
     1501 ttaaaccata caactcatta attttggcct tgtgttacca acaaggacat acactctagc
     1561 at
//
LOCUS       KCVB01000400            1761 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18605pri ultra-conserved element locus
            uce-1281, sequence.
ACCESSION   KCVB01000400
VERSION     KCVB01000400.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18605pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1761
                     /note="ultra-conserved element locus uce-1281"
BASE COUNT          521 a          342 c          347 g          551 t
ORIGIN      
        1 agggaaaatt ccagcgttta gtcttatcac agaaattcct cacttagttg acaatttcac
       61 caaactgtca gcgcaggaat ggagttattg ttattcgcca aatgaaggaa aacagccccc
      121 gtgtacagtg cgatattcca ctctcaaaat ccaaatgagg ttttctatcc tttcaatcaa
      181 tagccatccc ctgcagtgcc tgcatgtctc ttcacttcct aatcaacact tccactcata
      241 gggaggtcag atgagcaggc gcacagtgcc ccgagcgctg ctttgaactt aattaagcag
      301 ctttaaacac agccgaaaag attcattatt acacaggcaa gatgagcagc tagatgagaa
      361 catggccctt caacagagga gactcggctt cggatggctg ttgaacccac ttgggtcaac
      421 atcgagggcg cagcgataga ggtcccacga ggaccgccaa atcctgaaaa aatctgaaaa
      481 acctcttttt ttttttacag ctaaaacctc actataactt atgtaaatga tgtgcatgag
      541 acaatatgca cataaagatg taaacagtga tattatctgt taaagccatg caaaatcagc
      601 aacgagtgaa agagaaagag aggtaaagat ggaggtgctc atgcgcctgg aaggctgcag
      661 tgtgccacca gggtgctgtg aggcagggcc tgttaaagga gagctccaga ccaaggtgtt
      721 ctatagcttg ggggctaacg gtgataaatg agtcccttaa tgtcggcaaa cactttatct
      781 ctgcttcatc tgttttcatt aggcagggcc aacctttggc tgggcgagaa cgtgctgtaa
      841 ctttcattac tacaacacag aaaggagagg gagaaagacc tcccatcggt ctaataaaaa
      901 cactaggtag acaccatgac agatactatt tcttattatt atgttggcac gccaccgtta
      961 taagttagag aatgctgtgt ccctcaaaga tgatcagtgc ttttccttca ctcatgtccc
     1021 tgtctccccg ctgaatgatg tctgctcttt tctgctgctc ttattagtct gaaacgttct
     1081 tttgatctct tcccggcctt tatttttttg ttttttttac atgagaggga tgttgaatgg
     1141 gcaccaaggc ttttgcagaa gttgagttta tgtttgcagt ccttgtaagt tgatttgaat
     1201 gagaggaatc tagttatggc cgggcgataa tcagtagttt ttattcacct ccaatgaaat
     1261 catattctga tatgatcatt ttttgtagat aattttcaca aaaattaaat tcaatataaa
     1321 tgaatgttat aaattgaagt tttttttact taattttgtt tgcagcagtc aaaacgctcc
     1381 aggtccttgc agtgattttg ttttttattt ttaaattttc atgatataaa cacagtttta
     1441 aaagtgaatc tcttctgtta aaatttatat ggactgttaa gtttatttga cttttaagac
     1501 ttaaatatgg ctttccaaat agtcagtgga gaacaacagt gaatgttaaa tgtattaata
     1561 aacctacttt tttttaagtt cttacactat aaccagtggc accagtatta tatccatgct
     1621 agcagctgcg acaccagcat gacactcgtc tgaattgtct gtgtagctta tctttcaact
     1681 tttaaacatg caaataaact ttagagtaca gataaataga aattagtcta atttgtaaac
     1741 tgtatttaat ttgttacata t
//
LOCUS       KCVB01000401            1119 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18647pri ultra-conserved element locus
            uce-949, sequence.
ACCESSION   KCVB01000401
VERSION     KCVB01000401.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18647pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1119
                     /note="ultra-conserved element locus uce-949"
BASE COUNT          294 a          293 c          251 g          281 t
ORIGIN      
        1 ataatggcat gttggatgtc taaacacaca aagcatgtgc aggcccacac agatgcatgg
       61 atacatgcac acaattggag atactgtagt aaccccatta tggacactaa caacatacac
      121 cagcaacaag taattaagca ggcatctgaa aggcagaata atccctccac cttcagccat
      181 taacagacag aaagcgtagc agaagaaaag caggaaaagg agacccctgc actaaaggag
      241 acgccctttg aacttttgca acaagccctt ttccctaaac aaaaacacca aatcttcctc
      301 ttatctcctt tctctctctc ctggtctttt ttctccctcc tgctctcttg tttctttcct
      361 ctgtaaagtc agtggtccga ctccctcaaa ggaccagagc ccccagcctg ctcctattgg
      421 ttcctccttg ctctcccaca ctgagtgccc atggctcctg aagtgatgct ctttgtgtgg
      481 ggttcagggg tcgtgcctat tagcgtgaag aaagccctgc tgggtcttat cccgacccgg
      541 aggaggacgc agggctcttc accccaacgt cggcccggcc aacccccccc ccccggacgg
      601 gacactttca tcctttgtca ggaggtcaat gagcggaccc ccagagggga aggagggccc
      661 ccaccaataa tgatatgcat gacagtcatt gtatgacccc catggggggg tcatggttgg
      721 ttgaggtggg tgggacgagg gaggggagga ctggctgcgt aaccaaggat ggttatctta
      781 tccagaagta actgagttac agaatgatgg tgatgagggt gagagagctt cactggatcc
      841 tgatggatct ggttcatgat catatttgac atgaaaaatg tgggacagta ggaagttata
      901 acagcatcat attcaaatag gtttgtgctt tcatcaacaa tttaaatatc agagtaaatt
      961 attactggtt tgcatgttta tctctattaa gaaggcattc ctcgaccccc ccacacactc
     1021 acactcatct taacccctaa cctcaacccc agccttcgct tgagagatgg atttgatttg
     1081 gttcgcttca ctccctgcaa aacatttcag ctatttgtc
//
LOCUS       KCVB01000402            1767 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18688pri ultra-conserved element locus
            uce-377, sequence.
ACCESSION   KCVB01000402
VERSION     KCVB01000402.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18688pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1767
                     /note="ultra-conserved element locus uce-377"
BASE COUNT          572 a          370 c          392 g          433 t
ORIGIN      
        1 tccacaaacc acagacagaa ctggaaccag accagagcat cctgcatcag ggcatcggat
       61 agtagttaaa gttctggagt ttgaaaaccg attataattt tcccacaaga gaaattttcc
      121 ttgttgaaaa ttaaaggagc gtttggaaca actttactgc agagacatga acccagattg
      181 actgttcatg cacagcatga accaatccca cagtgggcct tgtgcaagaa ccactagatt
      241 tgttcttaag tggcatgtag agttgattta agataacttt tcttgtgctt agattgtttc
      301 cttgggtacg aacaaaattt acaggtcttc cagatctccc acaaaagtca tgtactacat
      361 atactctgtc taattccaca ggaaaaaaac acttgcttga ctttcagaat caaaatgaga
      421 atcataaagc cctcatctca actaatctaa attaaaaaac tgaggtttgg gataagaata
      481 caaaactttt cttgcatgag gcccactgtt atctgttaat gtgtcgggga acttggctga
      541 ggttactttg cgccactctg cattaaaatt aactgcgtgt ttagccagtt aattaatttt
      601 gcacctcaag gctttgaaat gaaagatgcg tacaaacggg aaactaaatc agaacgggag
      661 caggcacaac acaaacacag accagtggga caaaggagaa agagaaaaac tgtgcagtta
      721 cctttcccaa cattcttttt ggtagctgac gaaggagagg gaatcatagg taatttcaac
      781 tcagcacgat ttgactcagt cagaaggtag tgggacttat aggcatatga acttaatatg
      841 gtgtcagtaa ggtcttgcac taacagccct acacaagaca cacaggaaga gacgggggta
      901 agccgcagtc aaactcgaaa aattacccaa aaattcacaa aaagaagaga aggaacaaac
      961 atacacgcac aatctctctc acacacacgc acacacacag aagaaacagg taaaacaaag
     1021 tttgtcccaa cacaaagagg ccaacagtgc actcaacttg ttgtttcaca ggcaaaacat
     1081 cggatcttaa ctggttagaa gccattaaaa aagggtaaat tcctgtgttt tctcactcat
     1141 caacatccag gacagatggg actctttgaa gtggagtgca gtgtaacagc ctggatcatt
     1201 catactttta gtattgtgat ggaaaacaaa cattacattt cacagcactg ctattcatga
     1261 acaactatgc caaacatgaa acatagaatt tgaggaggta aaatatcaaa acttaaaaca
     1321 tctaaacaaa gtatgtgagg tttcagtttt cactcatgag atgttactcc aaaaaatgat
     1381 gcaaaaacta cagtcatgga ggctgtgtct cctgctggtg ctccatatga agtgcaagta
     1441 aggcgagtcg atcttgcggt ttgtcacagg actgaaatca gccagccagt catcaagtgt
     1501 gatgaaacta agaacttccg gatcagtctg ctcagtctgt tcagggtcac tggtgcatca
     1561 cggctgtggg agaggaaggc cgttagtgtg ttcaggtgtg agagcttacc tctggcacat
     1621 aggaagcacc agcccacgtt gacgggagag gtgaggaggc cgttcctctt ggcgctgcca
     1681 tggctgctgc agatcatgat gtgatgggtg agggcgacgc tgcctgcagc cacgcagctg
     1741 tctcctgtgt gataggccac cggacag
//
LOCUS       KCVB01000403            1406 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18730pri ultra-conserved element locus
            uce-332, sequence.
ACCESSION   KCVB01000403
VERSION     KCVB01000403.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18730pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1406
                     /note="ultra-conserved element locus uce-332"
BASE COUNT          378 a          358 c          281 g          389 t
ORIGIN      
        1 agccaacagt gcaaagctca catccatatc aaagcatgcg ggtcacctta gtccatgtac
       61 acatcggcct gctcagtgca caacggtaat aaaaagtaga actgtaactc acaaaacaca
      121 ttcaaatgca gccctgcagg aatcaaaact taatatcatt cctcttcatc tcctccaggc
      181 agcctcatag tctacctccc gagtgggaag aaaaaaacct tctgtgtccc taaagtatct
      241 tattgttatt cctaatcact cctcagaatc ataaacaatc tcacaacttt ttttttttta
      301 ttattcagcc ctctctgttt atgcagttcc cataaggcat tgccttttag tgtctccctc
      361 tccggcggca aagatgtcag gaaaacaaat aacggcggaa aacaaaacaa aacaaagtct
      421 cctgaaatac cttgtggatg cccagaggaa ggcgctggac agacagatga gcagataggt
      481 ttcctctgtc ctcctctctc aggctgcctc ctctctcggc gtgctgtgga gagtgactgc
      541 tcacagcccg cctggcatat ctaattagag ccaggctgtt gccactatcg ggcagaggat
      601 ctacctctcc aatctctcct gactcaatct tccccatctc tgattttctt ttatatttat
      661 gctggaatat ttaattgtag acaaaaggtt actctgggct ctattcatcg cacacaggca
      721 gaataatctc ctttaaaagg cttgacattt caggcttttc aaaaggggct gaaaaaatta
      781 tatccccgct tttgttaatc aatgaagtag aactcccccg aacaaaagaa ttaaattgct
      841 tggttgggtt aataaaacac acagaaatag ctgcttcctg tgggcttttg tgcctgccac
      901 tggctccccc catatgcaac cagcactgta attccatatg atttgggggg caaaactctc
      961 atttctccac acagttggct ttttaaatcc tattgaatga gcagtctgtg aggctggctg
     1021 attgggacct cttgcctgag cctggctgcc tggctgcctg gctagcagaa ctggctcttt
     1081 ccattcaaat atgacttcca ctaaggagag acagcgggtc cactacaatt tacaatttct
     1141 atactgcccc tgcctcagaa aaacattgta attatgcaaa tgataggggc aataattagg
     1201 cccttcactg tcccattgtg ttagcttctg tcgtgtttcg gtccaatggc aggagagacg
     1261 cagcagggca cgttggggtt taaattgctc cttacagaca gcctgcagat gttcacagca
     1321 tcttgtgtcc tataactagc catcacccca agccagggtg cctccatgta cgacactgta
     1381 gaaggagact ctggtttatc agtggg
//
LOCUS       KCVB01000404            1321 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18773pri ultra-conserved element locus
            uce-72, sequence.
ACCESSION   KCVB01000404
VERSION     KCVB01000404.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18773pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1321
                     /note="ultra-conserved element locus uce-72"
BASE COUNT          325 a          294 c          243 g          459 t
ORIGIN      
        1 aaaaatagac ttctagaaga gtgtggcatt gcgtctttta taagatggtg gcaaactgtc
       61 cagcaggggt tgtgattgct gttgttgcca tggttgtcct tacagtatgt cctctggtgt
      121 cagtcaatag tttgcttcct gtaggtttga ctcctctcct ctaacacatg acaagagacc
      181 caccgtgtgc tctgttgggc tgactcccgt ctcctctcct ctctgttctc tcctctcact
      241 tttttctctt atcctggggt tatccacata tgacacctgc cagggcggca ctgtagaagg
      301 aatctgacat ttcattctca cctgcagata cgacctcttc tcctttctca ccttgagtgt
      361 ttacactcat ttgcgacctc caagtgtcca cagtccagcc ccccgaaaca ctgtccaaat
      421 gtcagggaga gctatgaaac tggacctgtg tgagaccacc aagcggaggc tcatttttat
      481 tagtttagtg tgaactacta gcttaaaccc actttttgtc atcctgtctt gcccatgttt
      541 ctgaaatatc cgggatcagg ccacagggca caggtgaatt tgttgcaact gacattttct
      601 gcagtatagt gttcttcttc ctctcctctt cttcttcttt tttttttcag gctcagtgaa
      661 ctgagggaaa atgacattta attacaggag agcagtcacg cagttcggct aattacgatc
      721 ccgggagttc agtcatcata tctaattaac tttcactttc atgaaagctt aatgcagcac
      781 catagctagg cgctgcatgt gccacacttc aggaatattg acaactcttt ggaaattaat
      841 ttcaaaataa tttttttttc tttctctcgc ccttcacttt caaaacaaaa aaaaaccagt
      901 agagactcag tgttttttaa gccttaattt tacactccat gttttattta tgggactgta
      961 ggagatattt ataattgatc tcaccctgca ttatgcttaa atagaaatga atataatcta
     1021 tcacttggac ggtggtggca actcatagcc ttattgattg ttaaaagcac actgcatagt
     1081 ctgattcaca ttcattatga cactctcaga ggacaatcaa ttcattatta tgcttgccct
     1141 tgaccacatt gttagtgtgc ttactggggc ataatcctcg aagtgcatgt gtgcactcat
     1201 tggtgttgtc atttttcata gactcagact tgaagattta cttctggctt gttttatagt
     1261 cctttgttat tagcatttta atatggctat tattattatt cttattatta ttattattaa
     1321 g
//
LOCUS       KCVB01000405            1369 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18814pri ultra-conserved element locus
            uce-835, sequence.
ACCESSION   KCVB01000405
VERSION     KCVB01000405.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18814pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1369
                     /note="ultra-conserved element locus uce-835"
BASE COUNT          489 a          259 c          287 g          334 t
ORIGIN      
        1 cacacattga cagagtctca atgtgaggtg tactgaaaag ttcacatgga tggaagaaaa
       61 ggacagaaga gaagaaacaa tgaggctttc agtagatcag cgtttagata attgacaaca
      121 ataagaagag cagggcaagc atattgctat tagaaaatct tgaatctctg caactgacaa
      181 gttttccttc ttaagaaaaa gaaaatctaa ataaaaaggt aaccttgtcc caaggcgcct
      241 tgtacgaagt cccatgaaga tggagcggat gagtttgcca aatgaggctg catttacagg
      301 gtccagtttc tgctcctggc agtggcgcag ataatgattg tagagagtgg atcgtggtag
      361 acttacgccc tctgctgtct cgtaattgtc caacagccac tggagctaca agggaataca
      421 tgctttttta ctcaagagat tcacgctctg gacaacacaa actgtctgga aagacctcag
      481 actggcagtc tgtaatcttg atgatgcagc atgggtcaca cctctgcaaa agccttccta
      541 tagcagccag tgaatttaaa gcaatagtaa caattaaaac ttgcaaaaac aacagcaagc
      601 accaaatcaa acttaaattc atacagagtt aagaggagca gaagagcaag agagtgattc
      661 tagcaaaagt caaggtaagg cgagggcaag cattcgtaaa agcaacttac atggctgttg
      721 agcagcgagc ttctctgact ggataaaccc tcagattttt ggagcgtctc aatcgccatt
      781 tcaatctgat aaaccatgaa gcacaggaca acaaaatgta agaggtggaa ataaaaacaa
      841 aagcaagaga aataaagagt tgatgtagac acattagtgg ccactagcaa aaataaagag
      901 aatacagaaa ggatcccaaa gcaagcacca ctaaacccaa gcagagcagg actatctggc
      961 cttataggaa aatctttgcc tagtacatca atcgtggata attggcagac acataactcc
     1021 tcaagattcc accagtacag atccactatc atgtgcagag aaaaagccag actgctttac
     1081 aaaacaaaag actacggcag tgtgccatga tgattcagta acacctgcat gtctatctca
     1141 agagttaagg caaaaagctg gttaatgctg cactgggaga agtctaatta ggtattttcc
     1201 ataactagca aatcaaaaaa cctttttttt ttgtacattt ctgtcaaatt agcttttgtt
     1261 tcaatttaat ttaattggtg ttgtttatta ataaaaacct gtatcaaaac tgaactgatt
     1321 aaaaagtgaa gttattgagc agataactgt ggtcgaatac catggtcat
//
LOCUS       KCVB01000406            1654 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18852pri ultra-conserved element locus
            uce-870, sequence.
ACCESSION   KCVB01000406
VERSION     KCVB01000406.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18852pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1654
                     /note="ultra-conserved element locus uce-870"
BASE COUNT          474 a          331 c          395 g          454 t
ORIGIN      
        1 gcacttaaag caccaaaagt taacatgcgc attccctatt ttaaaatgat ttaaagctgt
       61 ttttattact tacaaataca cgtaacaaca ttttaatgtt ctagccctca acatgaagcc
      121 agttttagat actttgtata ctactgggca gattagtagt atagtactgc atcatatcat
      181 gttagcagat catgtgtttt gagtgtaaaa atgaatagta actataagtg tgaaataaat
      241 gtagtggagt aaaaaataca cattttccca ctgaaatgtg acaaagtagc acagaatgga
      301 aataatcaag ttaagtacaa gtatgtactt ttacttaagt tctgtacttc agtaaatgaa
      361 gtttcattcc atcactgagt acatgtattt tgttactttt cacgtctgga tatctgctct
      421 aatgttgcag gcaactgctg catttatcca tatttaagaa aaactgtggc ctactggtca
      481 gtgcaagggc gcagagaaag tggcttaagt aatgctgaac acatggtggc tgaatgtgtt
      541 agctcaaagc agtgtccggc tcgacttatg tcagttcccc accatgggga acttgacgtg
      601 tgggaataga ggctccatgc atggctgcca tgcattggcc cttacgtttt gcacatgtag
      661 gtaaacaatc gcgccttgtc tactgagagg cctgcgttta tacatgagtg caaaacatgt
      721 gttgcggcca aagtcgcatc aaaggtcact aagctatgct ttgaacactt caaagctggg
      781 ggatgagact gtagctttta agttttatgg caggtgtaag caggggttgc tgaccagccc
      841 agggtggtct gctccctggt ctgcaagagg ctgctggtgg ttagcgtggt agaagtgatg
      901 gtggtggtgg tgtgtggagg gccaaggagt cgggggcagg gacaaaaacc gaccgaccag
      961 gctgtctcca aaatgtcttc atgcagcagg ccacatttgg agtccttgga tctgaaaggg
     1021 tctctgggag cagactggac acaggcagac gtccacttca agggtctctg gtttcagagg
     1081 acctcagcag ggtacgtcta aggcctgtgt cagtcccatt tggaaacaga tacattggca
     1141 gagaggagcg gaggggccaa agcctgatga ccataccaca gagtgaccac aacaccgcac
     1201 cctccccgga cagaccagaa acagacccta tagcaagttc aaaggatcca aaggggtctt
     1261 gccaaagttc atttgctgga gttcattcat ctcagcacct gatgtggttg actgcttctt
     1321 gtttcgtgag gcatttcagt ttaaagtgtg agacttcaaa ggtggcaaga gttcttaatc
     1381 ggaatgagcc attctgtctg catcacccct ataaagttta caccccttaa ggaccatcca
     1441 atttcattac taaagaacaa aaatcgattt tcagatactc ttctgagctc attctaataa
     1501 cctcatcaat agatttatgt gatgaggaga agaatccgaa tatagtgaca agagggggat
     1561 ggcagcatgg gaaggttttt agaggttttg ttgattcttg gcttccactc acacaatatc
     1621 cacaacaaaa tggatattgc ttaaaatgac tcta
//
LOCUS       KCVB01000407            1233 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18891pri ultra-conserved element locus
            uce-37, sequence.
ACCESSION   KCVB01000407
VERSION     KCVB01000407.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18891pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1233
                     /note="ultra-conserved element locus uce-37"
BASE COUNT          405 a          234 c          290 g          304 t
ORIGIN      
        1 ctcaattaga agtgggccac aagtaattgt ttataacctg ccatacagcg tttacttcca
       61 gtctaacctg ccttcaacga atgacctgac ttgcactttg caggtcggtg tgaaaaggtt
      121 atgccaacgc tgctatatat actacatggc cgttggattc tgattgttgc gtgggtgaga
      181 ctggtccttg gtggcaaaac ctgagaactg gatcagcctg taaaacagac acatgtctga
      241 acaccatgtc agttggggac aggacacagt acactactgc aaatataaaa aaaataggca
      301 ggtaaactgg tcaaaaaagc tattctgttc tggctaagtt tacttcattg atagatgcaa
      361 gtaaactaga aaaaaactca aagtataaat gtatttgtag ctacttggca aaaaatagtt
      421 attacagttg aaacctttgt agcaagacca aaaatacaaa tccttttgga cacccaagag
      481 aaaacaagac gatgttggct gttgggaaag gcccgcccgc ccccttcagt gaatcttggc
      541 attgggattg gctgtctgga gaggggcagc caaccaatgg ggggagcttc ctgaggtcat
      601 gtggttggag gaggcagagt tcagaggttc tttatgtgac ttaccagcat ggactaccct
      661 ttaatcaaag agtagagaga aaggacaaag ttaaaccaag ttccctgggc aacgcaaatc
      721 aaaaagactt tacagcaaag ccatgtaagg gcagacaaga gagtgagaca cgtaacagga
      781 ttagacaata agacaatttc ttcatcagaa aaaaaaaaag aagaaaaaaa agagaggtgg
      841 aacgggttaa aatggagtga gggaatgtcg cagtcagacc tgggcaaaat ggttgttgaa
      901 tgcttttcaa atgtgcttca acacaagggg cacttgagta tcacttatct gacagacgat
      961 gtttccacct aagagtgtgt cccaagcaga taaatcaagt cctcttcaag taattcaacc
     1021 atttgaaact cgttcaactt aattttaagc ccaggtctgg tggaacttct tgcagacatg
     1081 acaggttgct gctgtggggg atggtcagtg gagaactagt aaggaactta ccaggcggtc
     1141 tggaccagcg tctgattctt ttcacattcc aggacttgaa ggtacataac tataatctga
     1201 aattacaaaa agattgcatt aaaaacacag ttc
//
LOCUS       KCVB01000408            1342 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18940pri ultra-conserved element locus
            uce-1044, sequence.
ACCESSION   KCVB01000408
VERSION     KCVB01000408.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18940pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1342
                     /note="ultra-conserved element locus uce-1044"
BASE COUNT          364 a          309 c          321 g          348 t
ORIGIN      
        1 aaaaaaataa cataagagtg tcaactgtcg cgtttagtca tttagaaaac atataggaca
       61 cgaagagctc aaaggcggca catgacaaca gagagagata agcataagat ttgtactaaa
      121 gtgagagtag tttggctgaa cacttgctag tctgttcatt agcaagtagt ttcctacaca
      181 tgtagctaac cgagccacgt gtataagcta gcgttagaga aaatatactc atgaatgaac
      241 atacaaggca taaaagtgac cctcattaca aaagcattta tcgctggctt aactaaatgg
      301 ggaaaaagta aaattctgtt agcattagga tgctatgggg cctataagtg caacaagagg
      361 cgctaagtgt tgatttagca cacctcactg ttatcaataa caggcgctaa actaattaac
      421 atgcattgac tggtataatt atggcttata aacacagcta attgcgttag tctttattct
      481 tgttgtcata gcccgtagtg tttctcaaaa caatgtaaaa ataatatgta gccaggccta
      541 agctaaaaca catatgctcc ggtgtaataa tcaagagtga agggggggtt catcggctca
      601 attagcggct aatcaaccgc cggggccgtc gtgtttcaaa gactcaccta agacgaaaaa
      661 ggggagctcg gtgttttcca ggtcgtccac caccatgacg tctccgggaa aatcgtttct
      721 aacggagaac agcagcttgg gctcggaggc cgacggactg tgcatgatgc tcaagtcgtt
      781 ttccctcaga aacggcagcg ccgacacggt gacgctcttc atcttgcact cggtgtcctg
      841 gctctggtcc tcgtcgccgt taagacactg gacattgggt gttaagacgg ccagaaggag
      901 ccacgctagc cccggcagca gcagcagcag cagcggtgtc ggccccatgt tgatgctcgc
      961 catctccgtc gctccgtttc ctctcccctc ctcgctgttt ctctctcagc tctcgggctg
     1021 cttcgatctc tcagcccggc ggatgagaga gcggcagtgt gagtggaggg ggtgtgttac
     1081 accggcgggc tttcacagtg tgggaaacaa acatctcttg tttctcacat gcgatcaatc
     1141 acggggtttt tgtatatgac ctactggaac attgactgtg tatttgtttt gcgtttttct
     1201 gctgttgacc cctttaattg atcgataaac tgtccaaatg gctctgtaat ggctaagcta
     1261 ccaacaggac agtcacctag aatgccccgt gggtcccatc accaaaagca gcatcatttc
     1321 tcatttgatt gtagactaca tg
//
LOCUS       KCVB01000409            1423 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 18970pri ultra-conserved element locus
            uce-603, sequence.
ACCESSION   KCVB01000409
VERSION     KCVB01000409.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="18970pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1423
                     /note="ultra-conserved element locus uce-603"
BASE COUNT          410 a          326 c          353 g          334 t
ORIGIN      
        1 aaaccaaaca caaaaacagt aacatctgca gccgcttcca gccagacagt ttgatgtgaa
       61 taactcagca ctttgtgccc tgaccttggc gaacaaagag ggggggagat gcctcaggag
      121 cgccgtggga cgccccggga ggcgagcgcc cagagagcac ccctgctcgt ttcgctgagt
      181 tggagttatt gtcccccggc agggcaaaga ggtgccacca gggcacccgg cactgcatgc
      241 cacttggact gtcatagttt cagcacaaag atggctgctt gacaaatgaa gcagagcggt
      301 gcgggaataa aatgtcattt tcagagtggt gcattgttgg gatcaccatg ccatatgaat
      361 gcagatcagg gtcaggcaaa taactatgaa gggtgccttg taaagatatt ggtaaatgta
      421 gagttaaatc agtgcaaggg ggagaagatg aatatttatc aatcctgcca gaggatgcta
      481 atatatatac agattaattt cacctcagtc caaagttaga aaaggcaaac tcctccccat
      541 caaatcaaag ggagacacaa caaagcaact gtgtacttca agacttaaat cttacaagtt
      601 cctaatcctt ctgaagtcca cacgggacct ttggtctcca tggcaacagc agggcattca
      661 accaagtgat tgcctccaat gaatttcaga taagacaaaa tggaagtgaa ctgatgtgat
      721 tttaatgcag aagtgagcgg tggaggggag gtccactcgg ttcatatttg aataaagcaa
      781 acagagcaga caaaaggctg acagccgtaa atgacattgt cagtacaaaa attatcagcc
      841 agtttacaat ctgtttgctc atgtagaaca aacagaatgt gtgtggcatc cccgggcttt
      901 gtgaacggca gggccggcag ccggcggcga gagaaaagca gatatcagct ccccctgcag
      961 ccctgcaaca attagatagc aatttgacag gcacactcaa aatgtgagtt tgttgttccc
     1021 ttccaagggc acagcatctt tttttttctt tttatcaaaa aggaaataca taattgaggc
     1081 attgtgaaca aagacaaatt gtgtgaccca tggccagagg actcgcaggg tgggatatca
     1141 caggcgtagg gagacacatt ccaaacataa gaccctcagt ttcctagcaa ggactcgaat
     1201 caaggtcccc ttttctgcct tgccctcctt tggtggtagg ggaccaacag ggagatcctc
     1261 cttaaagaca gccccggccc cgttcactcc ctgctgccct ttgttgcgtt ttgtcacttg
     1321 ttttgtttag agcttgttaa cttctctata gccgcatgga caagcagcag ctggtgagct
     1381 tgcggtgggc tgcaaggcat caattttatc cttcgacaag gag
//
LOCUS       KCVB01000410            1566 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19013pri ultra-conserved element locus
            uce-297, sequence.
ACCESSION   KCVB01000410
VERSION     KCVB01000410.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19013pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1566
                     /note="ultra-conserved element locus uce-297"
BASE COUNT          458 a          303 c          335 g          470 t
ORIGIN      
        1 taaagatgtc tgtgtttgtt tatttaatgt tttttgtgag aggagggtcc aagtctacat
       61 tacagtttgt tcctctttct aagagtgaag caggaagtga gtttaatttt ggatttgagt
      121 gctgtatatc actatttata taactgattt ttcttagttt agtctgggta aacgtctatt
      181 gtacacgcat gtaaaggcat agaaattaag atatttttta acattcataa agtaagaaat
      241 tgcatttgaa gcttaaatta aaacagacct tgcttctcca ctatgaacat cttcctttcc
      301 atcccttaca gggctgacat ttcttgatct ccaccagtat tgagtgaagc acacatgacc
      361 cctttgtcct tatgttgatg aatgactgcc tcatacccat tttgtcagat tttggtgcag
      421 tggagtcgat agcgtcacct tgacaaggaa agtttgtgat ccatcagccc tcaaggctgt
      481 ctggcctcaa ctaaggcctg gcaagagcag ctcactgtat gggggcatct actccatcca
      541 aaatccttca cccagtgtgc tgcgatcttg tgaggtcgaa cgaatggctc ttattactgc
      601 cgctttacag tgggggaaaa aaaagaaaaa aagaaaaaac acttgacttg tactaatagc
      661 tgagaagcca tgctggtcac caaaggtctg aagacgaagc accctgtcag tgcccagctt
      721 gggaaattag caaataatgt tcccttcccc ctgacaaatg tgtgacagct cgccttcgcc
      781 aatcattctc tcctaacgtt atgatgcatg tcctggatcc ccactggagg atacaggcga
      841 agtttactga ccctcaattt acccatgaca aatggggtcc cggtaaatgt gcgttttctg
      901 gggcgcggaa ggggttcatt ttaagcaatc ctggttatgt ggatgtcact gattaggata
      961 aatagtggct gagtgtgtta ggactgcttg cagaaccata ttaccaaggg cctgcatcgt
     1021 tgcccctgga ggagaactct cgtggaaact tggccaagat agaaaaccaa aagctctgat
     1081 gcaattagga gcttaaattg gataaattgt acagtcaaag tttttattta tgatttactc
     1141 tttcaaacag tgctgtaact atgcagtaaa aaggttaagg ttctgcaaat ggggttgaaa
     1201 attgagactt ttttgtttca gaaatatccg atcaaaccgg catgtactgt ttgcggtgct
     1261 taataaagtg gaaagtataa taaagtattg acctcaggta cagccaggac tttctccttt
     1321 tgattttctg caaccttgct gacagttaga ccaatggata accaatacat ggattagaca
     1381 gaggacaagc tagcaagctc taagggacaa cattttgttc caaggtaaaa ggtataggct
     1441 gtaaaacagc aaatatagcc aacatgtcat tcctttaaaa atgtgccaat taaaattcag
     1501 ataaagctcc ttgaagcagt ctgtaaagta cagcctacta aagttttata tagtatgtct
     1561 gtttgt
//
LOCUS       KCVB01000411            1012 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19059pri ultra-conserved element locus
            uce-646, sequence.
ACCESSION   KCVB01000411
VERSION     KCVB01000411.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19059pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1012
                     /note="ultra-conserved element locus uce-646"
BASE COUNT          256 a          312 c          233 g          211 t
ORIGIN      
        1 aatgttgctt cgatgtgaca gtttgtgccc tcaaccctga agccaaatcc agagctaaac
       61 cagtcaagtg tgccgcaaaa aacatttgtt gaaagaatac accagtaagc tttgacatgc
      121 agcaaaacaa caaaagacaa aaatactcac tagggatcat ttgtaaacct gggtgttctc
      181 ggaggttggt atccgtacag atggcaataa agcacgtcaa aacagaagca gcacatctcc
      241 gctgagacga ccatttttct gcctccactg cccgaaccgg gtcccgagct gagattcggg
      301 gagaggccgg atgaagtgta accggccggg gctggggaca gagcgtttgt gctgctgcta
      361 ccactactac tactgctgcc gctaacgtta cccccgggtc cccccaggcc gttggctctg
      421 ctcacgttcg ccgctgctgc aacggtcgcg gaggagccaa tccccaactc cgagccacaa
      481 tgtccggttc ctgccacccc gcttcccgcc cccaccccgc cgggaccccc cgacccgggc
      541 gatcccgaca gtttctgctt cttcaccccg cagcacccgg ccgccatctt ggaacaatct
      601 gcaccgacac accgcttccg acccataacc gtcaccgcgg gaagggtggg ggggaacgcc
      661 gaccagacgt agaaatccca ctgcgttgat acgtacgacg gacaatactc cactttcact
      721 cttttatttt tttgtcggac gacgaaataa ccgatgtgga gagaagactg caatgtcacc
      781 ctaacgttgc ctttaaaaat acgtgacttc gctacgcaaa atccatagct ggataagcgt
      841 tttttccatg agtagtcagc cagtccagct ttggaccacg gtgttccagt ggatcaccga
      901 caacgtcaag tcccagcaaa atattcaccc tttctcccta atacgacacc atccgaggct
      961 atctcagtca gttccccgta aacatacaag ttcagggagt gcacagtcca aa
//
LOCUS       KCVB01000412            1478 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19105pri ultra-conserved element locus
            uce-1159, sequence.
ACCESSION   KCVB01000412
VERSION     KCVB01000412.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19105pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1478
                     /note="ultra-conserved element locus uce-1159"
BASE COUNT          422 a          354 c          326 g          376 t
ORIGIN      
        1 ctgatgaaca cgttatatct gattacacaa acaagatgta acaagttaag gcaagagtta
       61 ccgtctccct tcatacacaa cctcctgtta tcatgtgact gaaacactca acgttacaac
      121 acctgagaaa ctttcattca ctgataagca gagttcagaa aaagagtgag cacatgtttg
      181 agttaagtta tgttcgatta gtttaaaaga gttgacatgt gagaacaggt cagagcgaga
      241 caacattttt tcaaattttc atgaactcaa aacgtcaaaa ggcagttttt tgtcttaatg
      301 agccatacgt ccctcattta aactccatgt taacaaacca cagaacacct tgaacagaca
      361 ctcggatgat ctgtctgtaa ctcaccctga ttggccatca cctcgttcat tccacttcct
      421 gtgaccgtct ggatgaaggt gaagtagctc ctcctcagca tctgcttctc cagagcggcc
      481 gcctggtcgt tgtcctccgc cggccgggcc aacacctcga agatggcgag caccagcggc
      541 atgaagacct gctgcaggaa tggagacacc tgccgctgcc gtggcaacag gagaaggagc
      601 acgttaatca ccttgtggtc tgtttgggac caactccaca tcaatatcac tcttgtccag
      661 tttaagataa cttctttgcc cgtcctgagt ttcctctgcg taccttgaac ttggcggtca
      721 tctggctgat gagcgggatg aactcctgca gatctttggc ctcgcagtcc ttcagcatgt
      781 gttcagaggc ggcggggatg aagggcagca cctcctcctc caggcagatg atcatccggt
      841 gcaggaagga gcggactgag ctgcgcagca ccccccgctg gacgggacag ctcagtgcag
      901 gcaggaaggt ctgcatacag tctctgtaga cctctgtaca gccgcactgc ttcaccgtct
      961 gcttgttgct gaacgccttg ctggtccgac tacaaacaga agacaaagga cgatacgttc
     1021 agctgcacag ttaagcctct gatttcagca gattgactgt aaatactgga aaccttcagg
     1081 tgttgtagag ctttaaacat ggaaacagtg gaaagaaaaa ggcagaattc tgacctgctg
     1141 caggtaaatc ccgacaaatg gtgcatttta acttcaacat attaaatgtt tttaacccga
     1201 gcaaacctac tgacatgaca tgaacctctg gaatttcaca ccaaactgat tttcttgcaa
     1261 caagttgtta ttccgtttga atgtatccaa gatggctttt tttccggttg atacagccca
     1321 aactagtttg tcaaaataac aaatgtgaca ttgaaaaaga accaaaccct gcaaaaggct
     1381 ctgcttatta ttacgtctga tagtttaagt aaagattgtt cgaacagaga gataaatgag
     1441 acagaactct tcagatccag tatggagaag aaaatgat
//
LOCUS       KCVB01000413            1510 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19146pri ultra-conserved element locus
            uce-502, sequence.
ACCESSION   KCVB01000413
VERSION     KCVB01000413.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19146pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1510
                     /note="ultra-conserved element locus uce-502"
BASE COUNT          314 a          429 c          248 g          519 t
ORIGIN      
        1 aattcaatac ttgaactgtt acttaatttc ctgccatcac tgtactatta ctctgccctc
       61 attgctccat gtcagagatt ctccatcagt gtggtactaa aggaaggaac gcattcctcc
      121 aaaacatatt tcctcatctg gtgtctcaat ggtggtggag agtgctgctt gacaccaaca
      181 cacccacagg tgttcagctg ggttgagatc tggtgaatgt gaaggacata gttcactttt
      241 ctctactcac tttgtttagg gttttatttc aattttcctt tcttttatca ctgtctgttc
      301 ttaataaagt tgtacagaat cacttcatgt cccacagtag attgttcgga ttgtcctgcc
      361 tccccttcct ttgctcactg acttgcagtc tcacctcctc attactcctc atgttgaact
      421 gctgaacctc caggtgtgct gctgaaccac ttcactcact acacagtcct cgcctcacct
      481 ctttctcctg ctcctcctct ctcagtttac ccacctcctt ctctccatga ctcgcttcac
      541 tcttcctcct ctctcccagc tctttttcct ttccacttcc ctgtctttac atttgtctcg
      601 cctccctgtc cactcttccc cacattcctc cttctcctgc ctttccatat cacctccctc
      661 ctcctccttt ccccctccct cgctcctcat ctctgtcgct gttggcagcc tatttaaatg
      721 ctccattgga gaaggattaa ggtttatgca aggtcagtgt gggatgtgat aattataatc
      781 acagcttttt gcctggccaa tgacggccat ttgttattct cagctcagtg gcttcagaag
      841 cccctggggg ggagctggtg ggaggtgttg acaagaggct ggctgattta ctgggcctcc
      901 ttctcccccc cccccccctt ccaacacaga gctggctttg ggctttgcat gtttccctcc
      961 actgtctcct tctcctcctc tctttagccc tcccttctct cccaattcgt cctctttgta
     1021 cagtatgtcc atccttctgt ctcctcttcc tcatctacac ctttattctt aacccacctt
     1081 ttccttttgc tctcctcctt ttcattttct ttgttcaagg gcatttctga cgtatatggc
     1141 agcagttgtc cctctgagga aagaaaatga gaaaaaataa atgtctggat tgttattgac
     1201 acacagctta tgtgggacaa ttaaggaact ttaaaacact aagtgcctga ttttgcttta
     1261 aaatcccact cgtcagtgtc atagctcagt caaattttga cacaaaggta tgatacacac
     1321 ggagtgactt cctctttccc aagatttaat tatctctgat gtttatcata aataaattag
     1381 ctaaaaaatt gtgaaaaagg tgctcatcct gttttaattt tttttcagta ccagagttat
     1441 cgagtgatag ccgtctataa gtttaatgta ggctgacagt acacagtctc atacctttga
     1501 cccttagtag
//
LOCUS       KCVB01000414            1325 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19188pri ultra-conserved element locus
            uce-990, sequence.
ACCESSION   KCVB01000414
VERSION     KCVB01000414.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19188pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1325
                     /note="ultra-conserved element locus uce-990"
BASE COUNT          394 a          232 c          269 g          430 t
ORIGIN      
        1 aatgcagtta agttaaaaaa aaaacaaaca ttgtttttta tacattattt aaaatcacgt
       61 atataaacac tgatcccccg agactcatct atattttttt tagcattgca ttgcatttgt
      121 tccttgtttg agcgagtgtt aattaaaatt gagatgaaag attcaataag aattcattag
      181 acttggacaa gtatttgtat atttacgttt ttaaacttga tttaaaagca aactggaaat
      241 caccaaaagt ctccatagag agttgttgtt atcagcagta cagttgctaa tgtaaatcta
      301 gataaaaggt cacagttagt aggtttctac aactaaaata tatcttagtg atgaatggat
      361 gaggctttgg atgtagctga cctttggctt ttccttattc cccaggcatt aagcctttcc
      421 agtgtgaccg ctgtgggaaa aagttcacac gggcctactc cctaaagatg catcggctga
      481 agcacgaagg taaacgctgt ttccggtgcc agatttgtag cgccacattc acgtccttcg
      541 gtgaatataa gcaccacatg agggtctccc gacatataat ccgcaagccg cggatttacg
      601 agtgcaaaac gtgcggggcc atgttcacca actctggcaa tttaattgta cacctgagga
      661 gtctgaacca tgaggcatcc gaactagcaa actacttcca gagcaggtga ggagtctggg
      721 ggcgccgacc cccattattt actcagatct cacctggatc agaaaataac ttcagtttag
      781 tttctaatac ctgaggttca tttgatcgtt ttttctttta ggactttggt tgtgtaaaat
      841 ttttgttgag atgattgcaa ttgatttgaa tgtccagatc aagtgtacct gaagtatttg
      901 tatgtattaa ttaagggggt taaattttac ttgtttaaaa gaggagtgtg acattttgga
      961 aaatgtgctt ttttcatttt cttcccagga gtcagataaa aagatatata tcactcagtt
     1021 tttttatgaa gtatgtatct ggagacagca gatgcttagc ttagcataaa gactggaaat
     1081 agtgaaaaac agcttggcct ttaacaaaat ttgcctgcca gcgcctctaa agctcagtta
     1141 taaacgtttt tgttttttca tacagacatg tacagacatg tttttatgta cagacactgc
     1201 agtgtaaaaa tagtattatg gagggttatg atctggacta ctttttggct caggtcaatc
     1261 ttactaagct tagctaaccg gctgctgact taagcttcat atttagctta cagaagtgtg
     1321 attgg
//
LOCUS       KCVB01000415            1492 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19231pri ultra-conserved element locus
            uce-547, sequence.
ACCESSION   KCVB01000415
VERSION     KCVB01000415.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19231pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1492
                     /note="ultra-conserved element locus uce-547"
BASE COUNT          438 a          345 c          296 g          413 t
ORIGIN      
        1 tctcctcctg tcagacagaa tactgtttga tctgcagctt ctctgtgtct tctatctttg
       61 gtcaaatgac ctcagcccac tagcagaggc tgcttgtaca gtcccactac tcccaggctg
      121 gctgaaagag tgaggaaaga gtgatggtgt tcaagtccat ctggttgtct tgacttagag
      181 tgtgtgttac atgcatggtc caacacacac acacacacac acacacatac cgtttctcta
      241 cctttgcttt catgtgttaa gtttgcacac acaggaacaa attcaagcat taaaaggtgt
      301 gctgtataca tatagatgca ctgcacatgt atgctacaca tgtgatggca tacacacaca
      361 cagtatacag tatagaaaca ctgcgcttga agtttggcct tggcccttgc tatacaacca
      421 gctagtgctg aactgaatga atttcgctgc ctctcttttt ctgcctcttt taccggactg
      481 gtgtctttta gcagctcaca tcacagagcc ctacaaacgg caattaaaac ggatgtgcta
      541 cactggctct gtatggggag agagagtaga gagagaaaca aaggagaaat gagaggggac
      601 atgagtgagg gagacagaaa gagagaaaga aagagaggaa gtatgcaagg gaaaatgtac
      661 agtaccacct ctccttcaaa accatgactg ataccgtgtc tattgatctt tattacaata
      721 gcagcaagct gtaaaatgtt tgatggtttt gtacagatct tagtccccct cattcataat
      781 tactgctaca catgtagaac caagggcaac ttcagaaggg gcgctgttta aaaagctgct
      841 gccagactgt agggatgatg ggaaatctcg acggggcctc acggtagctc caagccctaa
      901 ggctatcatt cctcctgccg aacacctgca gcaacttaag ttttacaaag atcaaattat
      961 ggagctgatt tcaaataaga aatgagacca accttttctc tttacccaca aaaaaaaact
     1021 ctgctttatt ttgatttcaa actttgctac taaggctttc taaaaaaaag tcagtagtgc
     1081 ttttgtaatt ttatgcaaag tgacctcaaa tctttcaagt ttaagttaga cattagaatt
     1141 taatgttgac aaatgcagat ctgagtttat ttcaatgcac tatgcatatg tcaaacgtgc
     1201 tcaccctctc ctccttgcat tgctttgtaa actgtgtccc tgcactcata acaacactga
     1261 gaagaagttt gagttgaatg ctacaacaat aactactcta actcataatg ttctacgcct
     1321 ggagaaacag ttccattcta gcctaaacaa acaagcagcc cagagtcttc acaacttatc
     1381 atcccatgct cttctttctg ggacaaaagt gtgcagcggc acaccaggac cccaggctgg
     1441 ccctggctct ccgtctcctc tctctgctca aattttaatg tcttctccga gt
//
LOCUS       KCVB01000416            1367 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19306pri ultra-conserved element locus
            uce-1199, sequence.
ACCESSION   KCVB01000416
VERSION     KCVB01000416.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19306pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1367
                     /note="ultra-conserved element locus uce-1199"
BASE COUNT          386 a          255 c          340 g          386 t
ORIGIN      
        1 gagagggaga gagagagaga gagagaggga gagagagaga gagagagagg gagagagaga
       61 gagagaggta aagcaaattg agatggttgg agctgagtcc ctggacctgg gccggtgtca
      121 gcagagcagc ccgtaaataa cctcttttat taggcccgta atgatcccca atgaatattg
      181 acagccagac tatgatgact ttgtagctga atatcggtaa ttaagtttca aaggagtggg
      241 tggtggaggt aaggtcatat tgatctccct gtgcgcaaca tgccagagaa caggacgtca
      301 atggagcagc ctgagacgtg gttgtctgca gcctgcgtgc ttcatgaatt cacacagtac
      361 ttgtgctgtg ccttcacacg gctaattccc ccccccctcg ctctttcttt ctcacacaca
      421 tacacgcaca tgacaacaat aatggagctc agagccattt ccgttaatgg gaattccggg
      481 catttccata acactgcaaa tgctctcttt tcaggagggt caatttgctg aattgtgatg
      541 gcgaaaatat caaaaaaggt acttggataa atctcacaaa acaatcagtg tgtgcaggaa
      601 gcaggagcgt agaggagtta gaaaagctct ttggtggctg gctggctggt tcagtgcaag
      661 tgaagatgaa taatttaagg aacacaaaga aatcaatgtg gcatctttag ttaatttgat
      721 ttgccgcatt agagaacaac tggatgcctt atctaattac acacacatat gcttgggtaa
      781 ttgttgcaaa gtcaatagta aacaggccgc attctttgaa caatttctac cactgctgct
      841 caactttggg ctgcatactg gctttgaagt cctgccctga gctgtagagt cagagggaat
      901 gatctaagct cgatgtgtgc tgcccagtgt gatgaaggct actaggttac ccttctctat
      961 ttgtacaggg gtggatgggc actgtgatgc aatgtagtca gatccacttt ataatgtttc
     1021 ctgagtgctg tgctgctgtg ttttccatct gaacatggtt gaatagtgcc gttatggttg
     1081 agttttctgc tgttgtttcc ttcaataaca cttgtataat atgtgaggga taatgcctga
     1141 tgagttgttc attatcagga attaatggcc tcagcagagt ccattaattc ctgattatgg
     1201 acaccttgaa gggcattatc ctgcttttac catggtgaca cggcaaataa aaataattaa
     1261 gaccattaat ttatattttc atgtgttttg caattaaaaa gtttttcaca agatgtaact
     1321 tggtttccat ggtaacacct gagggataaa ttaatacctg ggactat
//
LOCUS       KCVB01000417            1628 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19383pri ultra-conserved element locus
            uce-686, sequence.
ACCESSION   KCVB01000417
VERSION     KCVB01000417.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19383pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1628
                     /note="ultra-conserved element locus uce-686"
BASE COUNT          494 a          348 c          337 g          449 t
ORIGIN      
        1 atccccataa aacatcatcc catcagacag actccggatt gttgtataac tgaaagttaa
       61 aaatgctgtg cactaacaat atagctccat gtccattaaa tttgtgttcc aattaccatg
      121 aatgatgaac tttggctgac atgacgtatc catgattttt tggtttttca agcactactg
      181 tatcaggatg taaaactgga gcctccctaa aaatggaatt ttccctgctg taattgagac
      241 tttgatattg acataaatgc tatttacaag atgcaaactg gtaattagct ccaatatgaa
      301 aggaattgtg aattgaattg cacgtctgaa acccggattt tttaaatgag cactgatttt
      361 tatggatatt tgattctgtg aaacctgaaa atataaatcc tttatatgcc tgtgcatcta
      421 aagtgagtaa aatagaaata gatttaaatt gcattaaaca tttatatata ttcaatctat
      481 aaaaagctgt aatggccatt aacctaagta taaactttta ccctgactgc ctgcaatcaa
      541 agagaccggc agagctgcat gtcaggctgc gactagacaa tgaaaacagc agaagaatgc
      601 aaagtgaaac aacaacagaa acaacaacaa catgctgcat ggctcatgtt gaactcgccc
      661 cagaggtccc tgatgggtgg atgacttcag ctgcaaagtc actttgactc agtgaatcag
      721 gaatcaggta acgggatcag gcttcattag gtgacttcag ccgagctggc agacggccag
      781 gtgagctgga cgcacaaatg gacattgatt tttcaataag ggccctgaag ctgtcagcca
      841 accaggctat ggtgatatct ttcctcgcgg ggatggcctt ctcctgatga tggcctgact
      901 ggcacccgac cataggccac gacaccaaca tccacttcat ctccagctcc acacgtccct
      961 ttgggcctgc ccaattctgc cacactggtg ttaatgggta ttatcctgaa ggtcagtaaa
     1021 acttatgtgg ttatatagct gaaaaatggg catcaacata gatatgggac tggctggtat
     1081 gttgacgtaa atgttagtct tgtctttatg atattttcct agaaaagctg acacaagttt
     1141 gtgttgtctt tgttttattg tcttatttga cttgtataac actgaaaagg cactcagttt
     1201 acaaattggt ccacggtctc tgtggggtga cagaggttac aatcatattt tcctgtcagg
     1261 tcatccatat ttatctaatc tggtactctg ttggccctct ttgacccaga actaaagggg
     1321 cccaacaccc ataatggttt ccatccatca ggcctccctc tgtcctgccc tcagttttag
     1381 cagtcacaac ctctaagact gcagtcatca tgtactgccc ttacactgac ccccacaacc
     1441 agcagggcat catgcagtga agttggagga gagatggccc ggactcaacc ttctgtatga
     1501 ccctccaaaa tacaaatgtt agatgtaaga cggccttgat gcaaggagga cagcaggaat
     1561 gaaaaggaga ctggaaagaa aatgagacat tcacagtcag caataaagat agagagaaga
     1621 acttatta
//
LOCUS       KCVB01000418            1431 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19427pri ultra-conserved element locus
            uce-1265, sequence.
ACCESSION   KCVB01000418
VERSION     KCVB01000418.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19427pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1431
                     /note="ultra-conserved element locus uce-1265"
BASE COUNT          433 a          344 c          257 g          397 t
ORIGIN      
        1 tttgtccctt ctgacaggct catactagat aagacccgcc ctcttgatgt gtagcaacca
       61 atcatcataa catattatta cctacatata catacattca cacatacccc tgggtgtttt
      121 ggtaaaattt tcctgattac atttagatta ttattatact cataatattt tatcactatt
      181 aatgaggaca tttttcaaca tctatgtata tatacagtac atgacctgca actgaacggt
      241 catctcctta aatggcaggg tgaatcttgc ctcttgccca gtataaactg ggatttgcct
      301 caggcaccca gcaaccttca aggataagtg ggtatagcaa atggatgaat ggatggacac
      361 atatatgtta caaatgtctg caaaatccta caagcaatca aaaagatgtt caaaattatg
      421 aatgtgaatg tgttgtacca tcatgcattt catgatatca caacaaatat tttgcatcat
      481 cacctagccc tacacgtaca cacagaactc aatcacttgg catggcctca caaacgaaag
      541 attttggagg aaaaggtgtg tgacagctga gagtttaaga gattttacca aaaagaaaaa
      601 agaaaaaaaa agaaataaaa ggacttaact gcatcgtctc atatcaaatc tgacgagaaa
      661 tgatatctcg tcacccttca ctcaccccta tttgccgaga cccgcacccg cttcaccatt
      721 acacacaccc atttaacctg acctctcgct ggtgattaga agcctgtgaa gtgtgtgtga
      781 gcatgtgtgc gtatcggtct ttgtgtgtga gatgcttctc tccgtatgct aatcacagcc
      841 tagcactgag aattaatttg caaatgtgtc attacccatc aggctcagca gcaataaccc
      901 tgcaattaca caactctcgc ctggccagct gagcaccaca cacatgcatg caggcaaatg
      961 tatgcacaca cacacacaca cacgctgctt ctacggcact gaacaaaaaa gctcccatat
     1021 aaagacacta cattctgaaa aattttacat tctgctttac caccatgaga aaaccacaca
     1081 atactttgta cccaagtgat attcagcgtt tttacagttt aatcacagct cctcacctcc
     1141 gtgtcaacag gaccgcaacc aaggtcagct gagcactttc acacactcct gtttcttagc
     1201 aacattatcc gggagtgtgg cctcgctaag aactagagtg gcgtcacaca atgccttcag
     1261 atcaaagata cagtaactga gcaatgtgtg cgtgtgtgta tgtgtgtgtg tgtgtgtgtg
     1321 tgtttgttaa gtttgctgac cagcaccagg ttgagcagat cactagcatg aacactctcc
     1381 tcttcagtgc gagggtcttt aactttcatc tcttctgatc gctgctccct c
//
LOCUS       KCVB01000419            1492 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19470pri ultra-conserved element locus
            uce-1220, sequence.
ACCESSION   KCVB01000419
VERSION     KCVB01000419.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19470pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1492
                     /note="ultra-conserved element locus uce-1220"
BASE COUNT          503 a          258 c          265 g          466 t
ORIGIN      
        1 caaacatgga ggagagcaaa cctgaaacac agaacagggt aattctgaac aggagaagga
       61 ctgcaagctg ccaaaacaaa acgaaatact tagcacctaa aataggagct acttcttgtg
      121 taagggcgtc tttggggtat ataaattaaa atgagtaagt atctttgatt tgtccagcat
      181 atgattcaaa atgtaatagg aaataggcct ttcaatgtga tcatttgaaa ataaaagatg
      241 atttattcag aaaatgtgct atatcgtgtt atgtatcgtt atcaggatat gagattttgg
      301 tcatatcgca cagctctact agcttatact ccacatttct gttgatttaa gcagagtttt
      361 taaagatcag ttgcagtaag agaaggtcta ccagaaacaa ccatgtgaat taatgccacc
      421 tcatgattga aaacatagtt taagtttaca cactcacaag tgtaacatta attccaataa
      481 actttaccta aataatctaa ataattttgc tttaattata accaccataa caatgagcca
      541 cagtgagata aataaataac ttcactgtcc tccctggaat tgtgctgatg cggaggcctg
      601 cctaggcatt ataaacctaa ttagcagaag attactatgc agtgtgcaag gctgtggtcc
      661 agaaagacat cattaggtct tctgatgggg atatgccttt agcacattga ttctttttcg
      721 ctccaagaac aatgcagatt gcagacttta taattgggct ttcaggtcac ttgtgacttc
      781 acttccacca tgtgtgtctg gtgatctcca ttcaaacaaa agagccgata acaaaaagac
      841 attcccgctg agctcaagac atgtaaagga tgttcatgga ttatgagacg caccaacgag
      901 gctggatgaa taaaaatcat gtgagaaaca catactgtac gacatcataa gtattcaaca
      961 gaaatgataa tgaagttttc gatttaatca gattttggta cggttttgaa aaaacaaaca
     1021 atactgtttg aattaattta gcataatgga tcttaaaata tggatctctt atgactatta
     1081 catgtcaata atagtcttaa tgagacctat caacaaaaac cttgcattgc ttttcagatg
     1141 gaagcttaac agaagtggga catcgtcaag cagtctgttc ttcagcagcc tgattttgtg
     1201 ttgttcattt cttgctgtta tatcctcctt tctgtcactt caacttgaaa tgtatacaag
     1261 caataaacta ctcctaaaaa gaacacctgc tttaactcca ctgtttttaa atggtcgatg
     1321 attttagcag aaattatgtc tttgtgtcat ttatgtacac taccttaatt tctgcattat
     1381 ttggaagtga tattgtgaag tttatataaa caaatttcac tatatgtcaa taaatgtaag
     1441 attttaaata cattcatgtg aatttcatgc ttcagacatg taagctggtc ca
//
LOCUS       KCVB01000420            1064 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19510pri ultra-conserved element locus
            uce-1298, sequence.
ACCESSION   KCVB01000420
VERSION     KCVB01000420.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19510pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1064
                     /note="ultra-conserved element locus uce-1298"
BASE COUNT          348 a          237 c          207 g          272 t
ORIGIN      
        1 gtgaataaat tctaaatccc tcaatatctc tatcatttaa gagatactga cctccctcaa
       61 cacatccata aaacccgacg gacataccag gggaaaaaaa aaacaaaaaa aacaaaacac
      121 cttgccgcca tctgccaact gcacagttga cttgagcaca tgtactgtac ctaatcaaat
      181 ttagcagagc ctcactgaag gattaaaaat gtaaaaaaaa gagaacttaa atgccttttt
      241 tttcccttcg ataaagaaac aacacacaat atgagggcca atttgacttt caagaaagta
      301 cttgaataga tttggatttt aaacagcttt gtataccatt ctctccctct ctctctaatc
      361 aggcatctgt cactctgttt ttgaagttat tatcgtccaa gctccctctt ggatgtggtt
      421 gctgggtgac gggtatctta gcacccactc tgactgggaa tgatttgaat ggaatccaga
      481 aatctcccct gagtacctgt aaacacatcc gtgccttgta gaaaacaaat atattataag
      541 agagctcagc agaggagagg agggagagag acaagctggc accctcagtg gcagctcaca
      601 ctgagcgcta aacaaaacaa ttaccgtcat ttgcaaatat gtgtgtttag gcataaacac
      661 tcttgaaata ggaacaaaag aggtgatagc tctcaaaacc ctcgctgtca tatcagtatc
      721 aattaaatat atctgttcaa gaaaaagtcg tttcactgtg acctgtcact cgtagcattt
      781 cagagtaaga tctgtttgac tgaaacgagc cggggaaaat ctatttaaga agggcatgta
      841 agtgaaaagc cgtcccattc ccagtggtgg gacattgtct ggggcgtgtg gttaaccttc
      901 aagggtagac aatgattgtc aattaactgc tgcgtttctg caccccagca tcggatagcc
      961 tgcctccaca aagcagacca atgagagata tgcgatatca ccgcaccgcc ccctgtgtta
     1021 taataacctt tcagagagtt caagtgtcac aaagcaataa gaac
//
LOCUS       KCVB01000421            1543 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19551pri ultra-conserved element locus
            uce-113, sequence.
ACCESSION   KCVB01000421
VERSION     KCVB01000421.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19551pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1543
                     /note="ultra-conserved element locus uce-113"
BASE COUNT          429 a          364 c          340 g          410 t
ORIGIN      
        1 tattctttac catttttaca tccactgtta gctaaatgca caaagcttcc agagctggta
       61 ttgatccaac atcactggca gcagtattgt gttttaaata ccagcctaca atttgactga
      121 gctttttaaa tgggctgtgg cattgctatt aatgattatt aattatagaa actcgtagat
      181 aaacatgaaa cattctctgg agcagtaaac tggactcaga ccacttgttc tgctgttcaa
      241 acacactcaa tacaaacagt tccaaatagt ctttgtgcaa actaatggag gcaggtgctt
      301 ttaaatctgc caaatcaacc atatggcctc tgaggtggaa gtaacaagag tgagtgcaca
      361 gcagctctcg tttcctctcc acagtgaaac gttcagctca gcagactaca tcaaagctgc
      421 gggttctggc aacagatgtg acaaaggtgt tgtagcccgg agcagcccac ttgacctcgg
      481 ctgctttggg tgagaaagta gacgtcttct tggaaaagtg ttgatccgat ttcaacactt
      541 gtagctttat tgtcagttac aatatggagt tgagcatcac tctggtggag agtgaggcct
      601 cactggtctc gactctgtga caggagagag atggagatat gtctgtctgc aaacacatca
      661 actcagtgat tggtgggcat attgctcgac tcggggagtt ttcatcatta gcctgcactg
      721 catgtcaaac gggcggtgtg gccccttttt ttttccctta caggcggcag tatcacagct
      781 ctgatctgtc acccttcgct tgccttaatg tgtgaaagca agacgtgttg cgcctattag
      841 cacttcttgg caaggctggc atgctgatcc ctcatccccg gggagcccag caatatggtg
      901 ccgcatggtg accgcccgcc agcactggct cccgctgtca caacacatca cacatcccca
      961 acaaacacca ccaccacctc ctcttccacc acacctcctt cactggggct ggatgagtga
     1021 caggagcgcc acacagccag agccaggggt cctctgggtg gaagaacaac aaaaactttt
     1081 acagaaagga gggggggggg gggcttgatt gctaaaaacg cagcagttgt gtttggttca
     1141 atggaaagag aatggatcga attgatttag tgagggactg catctgaatt cacacatacc
     1201 agatatgttt ctgtgtttat acggtatgtc catcattgct ttttatttgc tgctgtcttc
     1261 taaacagggt ttttccttcc ttcgtccttc attctcaaaa aattcccatt aacacaacac
     1321 aggtcagcag actctcacat gcaggaggca aaacatgcag gaaacaaagt attctgaata
     1381 ataaaagcat ttgtaggcca agtccacagg tacaggggta ttttttaaaa caggttttta
     1441 aaggttttaa actcccgtct acacaagcac tgttttaaat aaaaaataca atagcgtgcc
     1501 aaaccaacag gcagcaatat aaacctaacc ccaaagccac act
//
LOCUS       KCVB01000422            1329 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19635pri ultra-conserved element locus
            uce-587, sequence.
ACCESSION   KCVB01000422
VERSION     KCVB01000422.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19635pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1329
                     /note="ultra-conserved element locus uce-587"
BASE COUNT          401 a          269 c          261 g          398 t
ORIGIN      
        1 tcgttgggta gtttaattaa agcaaacatc atactttata aactctttgt aatgtaacta
       61 gtaattgaag ctgtcagatt aatgtagtcg agtaaaaaaa tccatatttc cctgtgaaat
      121 gtaaagtatt agaagtagag agtagcatca agaggaaata cccaagtaaa gtaaaagtat
      181 ctcataattg tacttaagta cagtacttga gtaaatatac ttaattacat tccgcctctg
      241 aaatgaacag aatttccctg ttctactttt ctggtgtgac tgtcttaatg ccacctttgc
      301 actgtgcctc atctatgcag cataatgata cagtcagcac tgacaggatg ctataaatgt
      361 ctttttatca tgtggcactt ggcttacaca aggcctactt ttgttttgat ctctttttaa
      421 tgtgcactga cacttcctgc gaaccaccgc tgagtgaaat gcctcctctt ttcccacaca
      481 tcctctcttg atttttaaac atacatgctc tgttaataat gtgatttgaa tcattaatac
      541 gctacattgt aggcacccat tataattgga aaattagtta cagcacagtg tataaccgct
      601 tcctacaaca ctaatgccat ccctctctct gtgtcatcta aaccctatgc taatgaagcc
      661 tgggcccaca acatgcagga ggttgaatat caatttaatc aggccctccc tgcaggggct
      721 ttcagagaga aatgacagcc aaagtgcatt agtaattcta ctgctgtcac ttctcatagc
      781 tgcttacctt gcaaacaaac tgctctaatg gcacctgcct gctgggcaat ttaggcactg
      841 tagaggcaac agaacatgaa atggactgca gaccactgac ctaaatagag gagggccaac
      901 tctaaacaag gagtggctgt cttatcgttg gagtggaaaa aatgtgtgct atgtgtaaga
      961 acaattaaaa tgccttactg tgagtgcatc tccacctcca agacgcgaga aaaagaggca
     1021 acaaacctgt ctgtgcaaca aaaaaaggcc ccttgatcta atatttctgc caatttgtta
     1081 gtcatcaaaa gtgttttatc cttccatccc acatttcaga ttgatatcta cagataacag
     1141 gcacaaataa atactttaat acgcaaatga atagataaaa ggaagagttc attacctcct
     1201 ctcatacaat tgaattgctt tgacataatc ccctcttatt cctcaagggg atgggggagg
     1261 gagagagcat gatgggattg gtggtagtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     1321 gtgtgtgtg
//
LOCUS       KCVB01000423            1347 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19714pri ultra-conserved element locus
            uce-1121, sequence.
ACCESSION   KCVB01000423
VERSION     KCVB01000423.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19714pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1347
                     /note="ultra-conserved element locus uce-1121"
BASE COUNT          352 a          297 c          321 g          377 t
ORIGIN      
        1 ttttaggttc actttaagtt atttgcctta cacataacaa acttatgtga catcaccctg
       61 atggagcaca acaagctctg aatacattcc accgtcacaa caaacaatca caaaggtacc
      121 acatctaccc ggtccaaata gactttgaca ccaacgttga ggagctgagc ttctgagcta
      181 atttaaacaa aatggaccaa gttcctccga gccctggtcc cttagacgag tcctcctgca
      241 cgctgggctt ggctttgtca cctgtacgag gagaagaagg agctagcaac agatgtgtgg
      301 aaagtgtgaa tgtgtgatgt gttggtgctg tggcaggacc tttccgtgtg tctctctttt
      361 tctttggcag gaccaggagc ccctgcatgg gggccacccc ctctgacatc agcggggccc
      421 cgtagccctg ggaagttgag catgtgagct tccccaggtc attagggaga ggcttgaaaa
      481 gactgtcacc aggaaggaga taagccgcga ggggcgcagt aataaggccc tgaataatag
      541 cccctctgta gagagccact gaggacacag actcagggat taaagggagc ctgaaaagga
      601 gaaagtcaat ggacctccac aaaaatatga accagagtgc cggtgaatgg agggggagtt
      661 atcagcattc tgtgggctcc ttctctgggc cccatcaagc tcaaggagaa gggaggtgtt
      721 gggagtctcc tctgggatga gactggctat ttcaaaacaa ggtatttctg ctgtgttggc
      781 atacagcgtc aaagacacca caaagaaccg aaattcacat gtacttgctg accctgcagt
      841 tgactgaagt catgctcaaa tcttgacagt tgctgtgcat catcttttct cttgtaaggt
      901 gcatgtaatg tagaattcaa ctgaaggctc ttgtgatcac ttataaagct cagcatggtc
      961 agtctcctgg ccaacatcag tgatttatta cagcctctat gtcaccagca ggtccctaag
     1021 gtcgaatcag ggtttgctgg ttgtttctcg atcttggctt aaaatgaaag gagactgtgc
     1081 ttttgaagtt atggcttcta atcattgaaa ctctctccca tcggacttaa gatctgtgga
     1141 cactgtggac gcctttaaac aatagcttaa ggctcaattg tttccacttg atttgggttt
     1201 tttgctgttt cctgtttatt tctgctttta ttgtgtttat gtctacatgt atgtttaagt
     1261 attgttgctc tgaaacactt tgtgacatct ctgctctcaa aaggtgttat ataaataaag
     1321 ttactactta cttacataac gtgcata
//
LOCUS       KCVB01000424            1613 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19759pri ultra-conserved element locus
            uce-808, sequence.
ACCESSION   KCVB01000424
VERSION     KCVB01000424.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19759pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1613
                     /note="ultra-conserved element locus uce-808"
BASE COUNT          545 a          301 c          236 g          531 t
ORIGIN      
        1 tctgcaatta caatctaata atttcatata tgataaaata atgctaggga ccattatttt
       61 gcatggagta cttttacatt tgatacttta actacatttt tctaataata aaagctttga
      121 agtatcattt aaaatgcaat acttttacgt aatgacgtct ctctgtgtgt aaacagatct
      181 aaatacctaa tacatttgtc tgacacatag ccaaaagttg cttggaattt taagatgtta
      241 gataaaaaaa aatagtttaa aaaattataa atttatttag aataaacaaa aaaacaattt
      301 taaatgttaa aagtagctcc acctcgaccg actaggaatt gtaaaatgct actttcattt
      361 taatgcatca gaaataataa tatgataata aactgtaata atacacactt acagcagcct
      421 attttacttt tacttgcagt actttaaagc agcattgctt cttttactta agaaaaggat
      481 ctgcatactt cctgcatcac agcttagacc tcaaataatc ccaaactcac agttccacca
      541 tcaaacatct catacccacc tatttttccc attttccctc cagttttctc cccctgtgct
      601 ttggccgttg catccatgtc ttttatcaca attttgaccc acagaaagga attctcacaa
      661 gatatcaaaa caaaaatcta tatttaatat gggagcaatt acagagcgaa gttggctgca
      721 cagggccgtt gtcaccagag cgcgtgtttt tcatcaaata ggtcattaga cgatgatgga
      781 tgtaaaccta taaagtatct aaactggacc ttttaacaaa ccacgagcaa cactatcatt
      841 tctctaatag aacctgctga ccgtgtcatt ccctcctgcc atttgtcatt ttttcttgtg
      901 aaataatcaa tatctgggcg ctttgaaata aataatataa aagtcatagt aacctacccg
      961 aggagcaaca aaaatagact tatggaacat tcagaaggcc tatatggcct cagggcaacg
     1021 tctgcatgtt tgtaatcaat gcataatatg aactgtattt tctaaagtgc agactacagg
     1081 agcttagttt gtacagttta ttctcacggt ttgtatttgt tgtggtcttt ttaccccaag
     1141 ttcagcattt cagacagcca ctgtagtaaa gtattatttg tggcataaat atttcttaga
     1201 tcttcaacaa tccgtttgac tattcaaaaa cacaacctgg gatcaggcca atatgataca
     1261 tgtctgtaag agtttattca agggtttaaa taataataca gagcggagag taatgatatc
     1321 tgcacactga acagctccaa aatgagatat ttatttatgt caagcaccat tttaaccata
     1381 taggccttca tcaagcatca gtgctcccgt gtgtttaatt tccttcacct tcttttattt
     1441 tgatgtatta cttaaatgtt taaaattatt cacaataacc tcgaacaagc ctgcatttaa
     1501 ttttgccaaa aagaaaatgt attttgtcaa tggaagaagt aatgtaggtt gtcaataatt
     1561 tcaattctgt ttacatcaga tattcaaaaa attataggag gctgcacaaa ttt
//
LOCUS       KCVB01000425            1458 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19801pri ultra-conserved element locus
            uce-1079, sequence.
ACCESSION   KCVB01000425
VERSION     KCVB01000425.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19801pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1458
                     /note="ultra-conserved element locus uce-1079"
BASE COUNT          400 a          293 c          326 g          439 t
ORIGIN      
        1 cacatcaaca gtaatctaag tccaattatt gaacaatatg taaaactgac acacgcgtca
       61 ggtcggagac agaggaatgt acgagtgaac gaatgtgtgt taattcaatt cctacccacc
      121 gctcactaaa acaccaccac aaattaaatc ggccggggga gcagacggct aacaaaggaa
      181 attaacaatg atgctgcctc tgtattaggg catatggatt cgctgcatgt ttgctgcgtg
      241 tgtgtagccg cagcctcgcg cctctgcacg cagagagagg ggggggaaag gccagtttta
      301 atcaagaaat ccattccact tttctaaccc ctcttcgctt cttgctcccc tccctccatt
      361 gcaggcttgt gtggaaacag aagtgctaaa ttaccctgga gcagtcagac aaaaacatct
      421 tgttgggttc taaattgctt tgcatgcata ttaattggac agaaatccct gcagtgtatt
      481 tgggtttgcg cgcgcatttg tgtgtgactt caccggcgtg tgcatctgta ctgtatctgt
      541 gtatgtactg tatgtgcacg tgtgtgcgta cactctgtgt gtgtgtgtgt gttgtgggca
      601 gggctggtga gctattatag ctgcaatggc ttgcagagat ttggaggtta tctgtttttg
      661 ttggttaatt gtgaagctga tagtatcgag ggcctgggcc aattcgagtg ggctttcagc
      721 caaattacct cagtaatcgt ctgtattcaa gtccctggat gctctcacca agacagagcc
      781 tgggaagccc tgaagttcag tggagagggg cagatctgac cccagcttta gacagcttac
      841 actgtaattg aaatgatatt ttttttttcc ccacaccatt attggattgc tgacaagatt
      901 tcacccaaaa ttccatttgt ggtcaattta tcctgtttgt ttttaattaa tgcaggcagc
      961 taatgaactc aaagggaatc ttagctaaat gatcagaatt tcctccatgt tccctgttat
     1021 cttttaagca gtaggctttg gaattggagc atgaaattgt ttgaaagtag gaaaggaatt
     1081 cagttggatg gagtgcacaa acactgaagc aagcacaacg caagcagacg gggcattgga
     1141 tttgtaaatt cacatttcac tatgactaga aatttcatag actatgacta aaatttattt
     1201 gaaactataa ctagtttaat gtggctcaga catgaaggac taatgaaata tgaataatga
     1261 ctttttttta acatgttgat tcaattaatt caaaagtgga gcatgttgca tgtaagcagt
     1321 ttcgtgggtg cagtctcttt cttcatctat cttgtcatcc tctctcttcc tctctcaaag
     1381 ataaatcatc ccacagctgt ccttgtccaa ctagaaagtg ttccactcac tgaatgggta
     1441 tcgaaaaaat aacataat
//
LOCUS       KCVB01000426            1413 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19840pri ultra-conserved element locus
            uce-766, sequence.
ACCESSION   KCVB01000426
VERSION     KCVB01000426.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19840pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1413
                     /note="ultra-conserved element locus uce-766"
BASE COUNT          411 a          294 c          318 g          390 t
ORIGIN      
        1 atagggatgg ttacgcagcc agagactgaa gactgaaacc atgtgcttta atcatcatag
       61 tgttcattca gatgttagat gatgatgtct catgacaacc atgactatcc acctcaatcc
      121 agatgaacgt gtctgggttt ctgggctctt tatactgtat gttggcaaga caatgtaaaa
      181 ttatatgtat ggagtctcat ttaaaaatcc tatgtgctgt agaactgggt gggtctgtat
      241 ggcaagaaaa ttctgaaaaa cctctaaaaa gagtattttt aaactctgtc ttttgtctta
      301 attctctttc agataaatcc caaagtgaaa ggcatcatgg gtaaatatag gccaaatcag
      361 ggtcaaaatg acatcaaagc cctgagaagc ctgtggctgg tttgagctga gaaaacatct
      421 ctagatccct tctgtcttga tatataaagc tgcagggcgg ccaatcatgt agccattgcg
      481 gtagacagag gaagacggct gtggtggtcg attggcatta tgctggcggt tgaaatagcc
      541 ctcttgtttt tgttttcatc tctgggagaa ccattgtggc tgatgactcc tatttaaaag
      601 accatccaat atgcagggat gtcacctcaa actgcccata aacgtaattg cccgcccatt
      661 tgcttctgcc tttagaagcc cccagtaaaa gcaccctgtc agacgcgact gctgcaaagt
      721 ttagtgacaa gttccagcag caaaactttt atagatgcta accgagcaga agagaatatg
      781 aaggcagcaa cagacagaga gagaggtaac tactacttgt gtcatagtcg aagaaataac
      841 aggtgtgcct ttaatccctc acacttggaa aaagtgagat tacagcacag gtttagcgga
      901 gtgtgaatga gtgtgtgtat cagcactgac atgcaggttt aaaggatcaa aacagcaata
      961 caaaactgcg agacaaaaaa atgtctcaga tttcactctg acacgatagc aaaagaagca
     1021 caatgcaaga ctggaagagt gcgggcaaat tctgtctcca gcacacaact tgaaaacaca
     1081 actgcatttt aattttttta tgtgatatcc tgcttctttg agctcttcct gatgcatttt
     1141 gttttgttgt acgacaacgc ataagcagtg agctgttgtt gctaatggac cagttctgag
     1201 ggagcgcttt ctaatgcaac atctttggtg aatttggtta aaaccatctt ggaattacca
     1261 tagtgattcc cccatttgcg gttggtagtg gtaaaatgtt gtgtggtacc ctacagaggg
     1321 agcccttatc gcacgccagg tgtgtgggtc gtcaccccac ctgcgtgcag caacacgctc
     1381 actactcccc tctatgcctt gccattgcaa cac
//
LOCUS       KCVB01000427            1490 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19880pri ultra-conserved element locus
            uce-723, sequence.
ACCESSION   KCVB01000427
VERSION     KCVB01000427.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19880pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1490
                     /note="ultra-conserved element locus uce-723"
BASE COUNT          514 a          283 c          306 g          387 t
ORIGIN      
        1 tacacatgtc cactcattca aataagtctc ctccctcagg aaatagttgg tctgctaccc
       61 agctgcttgc ccttaaatag ccttgactcc aattctgccc ctatagtcaa ggccaagccc
      121 ctgctacaca aggagggggt atttgttggg aaaagaaagg gaaggaaaac acttaaaaca
      181 taagaactac aaatgtatta gaaataaagg caaagaaact ggtgtgcaat taaaattgat
      241 agtttaaatt taaccaatgt gcccagaact ctccatggtg ctgaatggca taagaataat
      301 cagatctaaa taaaatccaa ctctgcatgt aagaataatt gctattttct gtaatagaca
      361 taaaataaca catcatctgg gttaaataaa ataccaagga acagaacaca ttaaccacca
      421 caaggcttat gaatgatatt tttcttgtat catagaacat tgggtctgat ccactgtgtg
      481 cacatggaga tgcattcaga tcagcctatc ctcctcctct tccctccatc accttccagc
      541 ctctgtatcg gtgatagtgg ggtggcaggc aggatgaaac acttcctctg tgaagcagaa
      601 cttttatgtc aggtgaagcc agacacatct catccccttc aatagctcct cgtcaatttc
      661 aggcttaaaa ttttatggga taggatactg tttgatgcag tctaccagcg gtccataaca
      721 gcagtcattt attgtgcact gaaaagaggg ggaagaaata ttatggcgtg ggagaagggt
      781 gggtggatgg aagggtggtg agggaaggga gcccatacag tgcagagaaa ggagaaaaca
      841 cgggagatgc tggcagattg gcaggcatga ccgccggctc tcacgtgacc tcatcagcaa
      901 acatgagcgg ctgatgccgt ctgtcagcca ggtggcacaa tatcacttaa aaaactcact
      961 gtacataagc agtagatttg ttagatggaa aaagataggg caacaaaagt taaaaaaaaa
     1021 taaaaataaa aataaaactg aattgaatta ataaaaaaaa aaatacaatc cccaaacaat
     1081 ttcaattaat tcatgtaata tttcagtaca aatttaaaat gtaaaatact tacttggtag
     1141 atgttattag ccaaaatttg gtctggaatt aacgacggct ccttcagcat gctgttgagg
     1201 actgttttag aggctgtgaa tgacacagga aggcaggaga gggatagaaa cacatacaga
     1261 agtcacatta ctattagtat gcaaattgct tgtctaacct ataaaataaa aaatatatat
     1321 tctaattgct gcattgaatt gagagcaaac agtgaaaata cgcagacaaa agccatactt
     1381 gacatactgt aattcaccat gccactgttg gtgcagtatt tctacactag tccattagat
     1441 ggctgcttgg tgtctataca gattttctat agataaatgt tccagtccag
//
LOCUS       KCVB01000428            1825 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19930pri ultra-conserved element locus
            uce-422, sequence.
ACCESSION   KCVB01000428
VERSION     KCVB01000428.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19930pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1825
                     /note="ultra-conserved element locus uce-422"
BASE COUNT          467 a          370 c          431 g          557 t
ORIGIN      
        1 cttgagtgca ttgattcata acagtcatgt aagtacttat ctcccgggat attagtcacc
       61 catcctcttg atgatgtgat atcatatttt tcgctccttg tctcgagtct gtgccccagg
      121 catggagaca tatgcaaagc ccgacaaacc atagctccat aaagcccacc tttgtgctgc
      181 agtttacgtc cggctaagct gaatgtgaat gtgtttccac atgactaata gatgatgcat
      241 gctgtctgcc aggccaggtt tacctctagt cagaggccag acagtgtgcg atgagaacag
      301 gaaacccagg gtttttgttt ttgtttttgt gaggtcaaag aaagacagag tcacctgtag
      361 agtggagtca gcaggtgttc tgcctctcag gtctcaacac cttaactatc ttgggagtga
      421 ttatgtggcc tgcacactgg cagctggaaa ggggaggtag atttctctaa gcaagctgtt
      481 cttgtgtata cctgtaaaat gtgtaagtgg atacagatat aaataaatta ttgttctccg
      541 ttctctagat cctgatttaa gctcttatta ttaagaatta aatgcagctt atggtgaaat
      601 tttgatgcat ctgtgcgtct tgccctagat cacaacacca cttggtagag caaaccctta
      661 aaaagtccat ctccactttg atgaattata ttttctcagc aatgaagctt ccattgttta
      721 ttcttcctct ttctcagtgt tattcttttc ataccaggtt gactatttcc ccgttgagtg
      781 aataagcctg tcattcccat attaaacaca atgctggtcc tataaatgga caccacctgt
      841 ttaaacggtc agtgccatgt tagggtcaag cagggtcctg tttgattggc cacatcgaat
      901 agtcggaaca cgccgcatgc cctctgtggt aactttaaaa aatgttcagg aagtttggtt
      961 caccctttac ggcctaggac atttcctact acaggagtta agcactcagg acaggaaacc
     1021 atttgctgtg gctgtaagat ctctttaatg actgtctaac tagaaacagg ccggcctaat
     1081 tcagtgcctg gagccagcag gaatggcctt gcatctagac gttgttagaa tatttactgc
     1141 ttcttcgatg ggagtctttt tgacactgct gcaataaaat ttacaaggaa tcccttttta
     1201 gcgtgatgcg aggcaaagtg tggtcaagag gggaaaaaca tgcttgaaaa agtagcgggc
     1261 cattaaagtt atcatttgtc tttgtttatg tggactagga actccggatg gctgacattt
     1321 aaacagcatt tgatcggctt tgtccccggt tagctcggaa tgctatttgt tgatggtcag
     1381 catcctctga ctttgcagtg tgtcggaaag ggtaactgtg ttgaattgta cgaccttagt
     1441 tttagtatat atgaaagggt cagtgttgcc cttggggggg gtgggggctg ctgattcaga
     1501 catattgagc ttttaatgcc ctctaaggtt ctcttgactg ctgtcaaatg ttatacttca
     1561 aatcaaagtc ttgctgtcac aagagctgca ggacaaccat atgtaccttg gagatcggca
     1621 ctgaaaaagt gactcggtaa acatgagagt agttatggat taggtgtgtt tgtgtacatg
     1681 ttcatgtgtt acatgcctat gtgggtgtcc atgcaccacc gtacacagag tgtaagtgct
     1741 cccgctgact gaggaaatga gctgggacca ccagaggtga ttgtgcatgt gcaagcagag
     1801 accaggattg gcttgtttca gtact
//
LOCUS       KCVB01000429             729 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 19970pri ultra-conserved element locus
            uce-576, sequence.
ACCESSION   KCVB01000429
VERSION     KCVB01000429.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 729)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="19970pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>729
                     /note="ultra-conserved element locus uce-576"
BASE COUNT          207 a          168 c          156 g          198 t
ORIGIN      
        1 gtttctttct ctctaaggtc atttagaaag tgcttgttta gggatgagtg attagcagag
       61 atgtccaggg gcttcctact catctgaggc ttcctggaaa ggcagggtag acctgctaca
      121 gtggcatggc tgaacagtgg aggctctagg ggttttgcca tccaatcatc caatacaggg
      181 gggattctgc tgtggttgtg agcttggtga ataagcgtca tagaggctct tgtgtttaaa
      241 ttgcacgtgt tgttatattg taacagtgca ttttaaagtt gggttttgga atttctctag
      301 ttaaggtatt ttagcagtgt tttattccct gacccagacc ctctaatacc aagagacaac
      361 atgttaccac atttaaacga tggcattgaa catgcacaca catttatgca cacgcaggct
      421 aacacatgca cacaaacaga caagcattcg atgtaacaaa gtcctctgtt gaccctgaca
      481 tccaatttaa caaacaccag tttaaagtcc tgttgacaag aagcaggaag cgagaagaga
      541 aagaggatat gacatgagga gaaatcggct ccttctgttc cttgtcactc catattattg
      601 aagacttacc caacatgcac ctgggggaat cagtgtctgt ccacccgagt ctctctgtct
      661 catgttgctc tgcctctctc taacacacac acacacacac acacatacac acacacacac
      721 acacacaca
//
LOCUS       KCVB01000430            1152 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20011pri ultra-conserved element locus
            uce-533, sequence.
ACCESSION   KCVB01000430
VERSION     KCVB01000430.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20011pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1152
                     /note="ultra-conserved element locus uce-533"
BASE COUNT          349 a          254 c          227 g          322 t
ORIGIN      
        1 tctacaaaac attataaatg taaacaaatg gctttacatc taaaattgat gctcgctctt
       61 gcaaactgtc gatggaacat cagattatgc tggaaatcag gcatttttca atgagtatac
      121 tccaattcaa acaattcaaa cataaataaa gtcaaaactt tcaagtcttt gcacaaactc
      181 tggtggatat cagctgaaat ctgtctctct tcctgcagaa acaaacgtct gcatccacaa
      241 atcaacagga caactttcac aggaggcgaa cgctgccgac tggagttatg agtttctctc
      301 cgagcgtccg atccgctgca ctttgtttaa gtatctcttt ccatttgttg gggaggctaa
      361 tccttcgccg tactttttta caagctgtta atattgattg ccaccgcgga tgagagaggg
      421 gcggctcaat ctcaataaca gcattccatt atatggcaga cgacagccgt gccgagtgtg
      481 catcttccat gcagccacgc atttagggta ttatcaaatt gatagtgact caaattgatt
      541 tggataatga aggtcgctgc accttgaaat ataatggcca cttattgtaa ttaccgtcac
      601 aattatcaag gtcaccgcat caaaaacaaa gggactgaca ttgtgaccac ggcaacggag
      661 cctgatgagc ctttattatt taatgtggtg atactgtttg atattaaagt gataaacgca
      721 gacaaaaggt caagttttat atgattaaga agtgttcagt gtttgacatg tttgcagtct
      781 gtgcgtcact tgtctcaata atacattttt tatgggtaaa tacagaaaag atgccactca
      841 aagggatttc aggggttgcc agtcaaactc aaactgcaga tttcctgaaa aaaatcagtc
      901 aattaagcag atgatgctta actgattcga ctgagatcac taatttccag gtcaggagct
      961 acaaataaac tgacaacaga gctggaaatc tttgcttcgt tttaggaaaa cagaccacat
     1021 gacaccgtct cctgtatgat ttggacttgg gctgcctcca cccacccttc ctcctccctg
     1081 cctgcctctg atgcccgtca acgtgtcaga gaaagctgga atgacatccc aacacgagca
     1141 aacttgtcac ac
//
LOCUS       KCVB01000431            1698 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20045pri ultra-conserved element locus
            uce-1195, sequence.
ACCESSION   KCVB01000431
VERSION     KCVB01000431.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20045pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1698
                     /note="ultra-conserved element locus uce-1195"
BASE COUNT          425 a          401 c          371 g          501 t
ORIGIN      
        1 cacattacgc tggatgatag cagaggggtg cagaggaact caagcatgca atttgttggt
       61 agccacaatt taacctcatc tcctaaagcc ggctgttagg cagccagtgg gacatgtcag
      121 tcaatcggga atgctaatca gtgacggtgg agagaaagcc cccggatcgg catcgtactg
      181 attttcatta ctggctccaa gtcctcgggc cttcaggttt attttcacag catgacaact
      241 cttcataaca aaagggaagg ctaccacaaa ccattaaatg caatttacaa attagatttt
      301 ggctaaatct gtgaaatact ataataggct tcttaaagta gccagcagta aaatacacac
      361 actcagcaaa tgttttctat tcactttgcc aggtaacatg tttacatcag aagaatgaag
      421 ctcttttagt ggctgattca tttcagatat catttacagt gtgacagtga aggtgtacca
      481 tttaaaaata atttctgcac aattccagcc tttttaatga cgcttcaggc ctgttttttc
      541 tcatttcgct tctacagttt ggaaatagcc cacacttgtt tctccgggca gcctggtcga
      601 acgtgcatga gaaattgaga catggcctct gctgctggct cgcagtaccg ctgctggccc
      661 caactgtgta tatgtgatcc cactctggaa tgtttttcca acacgtaggc ttttcacaga
      721 tacactagag aggactggca tcatagtggc aagtgctgca ttcagactgt tgtgtgccca
      781 aggcttactc tcacactcac gtcacacatt ctagctctac atgggaatgg gcagagcagt
      841 gtgtttgtct acttacaccc caggtttttg agagggtgtg ggcgcatgtg tatgtgtgtg
      901 tgtgtgtgtg cgattgcttc ctctctctgc ttctttcttt ctttgtcttt aatctgagct
      961 ccttccactg cctcacattc actctctgta ctttaaagat acatttcagc acctcctctt
     1021 actcgcttca tgtcatattt gcctgactga atttcagcag aatagatttt tgtacagata
     1081 cggaaaaaca catttctttg tcttttcttt ttcttttctt tttttttacc ccttttaatc
     1141 ttcatcaaat gagattagcc cccaaatacc ggtattcctg cccaaagtga ctgacagaga
     1201 tttgcacttt tatcattaag tgtgacacta agctggtggg agctttgtcc tgaatggagg
     1261 gtctgacgga aacgggggct cctaatcctt ttatttgtgt caggcccaga aagggggaag
     1321 aatggggccg aggtgggatg gggctgcaga atgccacggc ccttagtccc ctccagaccc
     1381 gggcctccac agccaagtga gcatgagact catcttatgt ggtctatgca ctcgagcgga
     1441 cgcatacaca ctcatgcgca cacaggcaca catttgtgca tctacagtat gttaaaatgt
     1501 ttgctcaagc acatgcacac aatcaacaca cttcctcgtg ttcacatgca tatgttcacc
     1561 tgctgctgca cccaatcgct gatacacaca gacctgcaca gtcatttcag agagcgtagc
     1621 tgcaccgagc agagcgtggg tctgtctatg actgcatgga ttgcagttgc aggagattca
     1681 gtctaggatg agattaat
//
LOCUS       KCVB01000432            1243 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20087pri ultra-conserved element locus
            uce-1168, sequence.
ACCESSION   KCVB01000432
VERSION     KCVB01000432.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20087pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1243
                     /note="ultra-conserved element locus uce-1168"
BASE COUNT          371 a          264 c          222 g          386 t
ORIGIN      
        1 gaaatgagaa tattttccaa aatgacaaac agttacttta aggttgcaca aaataattaa
       61 ctgttgacat acatgggaat taaatcacac agcttattca taaaatttca catattttca
      121 gtgcaaacac actatgtgac taaagcttta tttcatgttt gttgctcctg ttgataatct
      181 ttgctgcatg tgcacttaaa aaacatataa cctgcactga aacctacagt cctttcttcc
      241 ctccatcctt aatttacatg tagaatccgt tttatatccc gttttctttc ctgctcatta
      301 catccctccc atctgccatc tttgcccttt ctgttacatc cattcatgtt acggtcatac
      361 ggccttggga ttttgtgtca agcacttctc tctacccctc ttcatcactt tcatctcacc
      421 tttttccttt gtacccaacc cccaccccca acaccccctc tccggttttg gccttccacg
      481 cttggaagaa cagcgtgagg cggcatggcc tccatgcggc tgactgatta ttgtaattca
      541 tacactttaa ttagctgcac aaaccattag ccacaattag actattacag aacgttttgt
      601 ggcaggagcc agcaccaaga ttggctttat ctgctgaagg ggggagaggg aaaaagaggg
      661 agagagaggt agagaggggc aggagagtaa aatgtattga gctaagtcat cagtgtatat
      721 gtgtttttaa actacaaagg gtaatttaga atgttttaaa aagtgtgtgt tttaaatatg
      781 ccttgtgtgt caaagggaag aaagtttgaa tcacaggcag catagaacaa caactgagca
      841 gatttattat tgtatttctg ctccagctcg acaggaaata gcagaatgag atctgaatct
      901 gttaaacaga aacatatagg caatggctaa tcataaacta aagacacatg cattgacaca
      961 ttctgaatct tcttttattc tcatcataat gaaatctttg tttgattgac acagtgcacc
     1021 cccacatttt ctctgccacg ctactgtaag agtgaatcac cagttcattt gtgtcacatt
     1081 tgtgcctgaa atggcagggt gagttgtatt attagacctt tgaaaccatt attgcacgct
     1141 tgacatatca acttgatccc tcagtcaaga ttacaaaata tatacatcat ccacatgaca
     1201 gttgacatta catggttgag tagattgagg actatatgaa aaa
//
LOCUS       KCVB01000433            1089 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20127pri ultra-conserved element locus
            uce-677, sequence.
ACCESSION   KCVB01000433
VERSION     KCVB01000433.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20127pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1089
                     /note="ultra-conserved element locus uce-677"
BASE COUNT          382 a          231 c          189 g          287 t
ORIGIN      
        1 cgagcttatg agacaccagc agttttgaac aaaagcagct cggtaccaaa tcaaaataca
       61 ggcaaaccag ccccaacaga aaacagttct ttgataaatg tgaacttaat tatcttaagt
      121 gccttgttaa taacagactg ctcatctctc agttatttct cataggcaga tttaatgttt
      181 taatgtgctg aattgcattt aaaaatcagt tataaataac aatgattcat accacaatat
      241 tattatgaag taattattag atataaagag agaaagtcta agaatagtgg aggtcatatg
      301 ttgcacaaat tgggaaaacc cactgagaca aacttgctat tttgggctat ttacataaat
      361 tcacttgatt tttctcaaaa tataaaatat ataatgctta aatatccacc catgcatgaa
      421 agtaatgaac aaagcaaact cccttcaccc aacaaattgc tcttgagtca ttcagggggt
      481 tgttagttaa ccctgagctc agaggatgat gaagcaaagg cataaatcac agagtgaaac
      541 aaggcagact gcttcgattc attaatggaa atgatctgtc aatatgctga agtcaattca
      601 cacgtgtcag ctgggctcag cacacaatag gccaggcaac ttatgaactc ccagcaccac
      661 tccaccgagt ccataaaaca actggcatcg ccgctgggga gcaccccaag acaccaccct
      721 cttgcacaga ccaccagatg acacagatgg ggcgaaacca aacaaagacg agactccctt
      781 tgtacaaaga gggacccctt tcaaaagcca ttcatgcaac aaattgggga aatctggcca
      841 tgcaacactc tgggtttgct cttttattag cgtgagcctt acgtcacagt aagtgaaatc
      901 agatttatgg ctctttgcta caaaaggtgg ttaaaatcaa gcacactttt aaagacacaa
      961 aaacttttaa aaccatgtta accaacaaaa gtttagctat gttaaccaga aacagtcttt
     1021 cattttgcac atgagtaaca gagtaactgg ccattcctct tttcacaaca tatttacatt
     1081 ttgtacaat
//
LOCUS       KCVB01000434            1409 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20207pri ultra-conserved element locus
            uce-1075, sequence.
ACCESSION   KCVB01000434
VERSION     KCVB01000434.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20207pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1409
                     /note="ultra-conserved element locus uce-1075"
BASE COUNT          535 a          235 c          213 g          426 t
ORIGIN      
        1 aatgtttgtg cggactgctt atgcacgtgt taatataaca ctacataact gagccaaact
       61 gtgaatacaa cacaggacga gtcgtaaata agcacaccaa gcacacattt tcatcacagt
      121 taagatttat ttcattcaac atttcacatt acaaatattt aaaaattatg catactgtta
      181 taaatagttg gctgcaaaca attaaataac attagatttt tctttttcgt ttcactagca
      241 cttctacaga ccaactccta gaaatatgga catatccgta ttacataact taattagaaa
      301 ggaaaataca aaaataggaa cttataaagt gaaatgacaa taaaaattaa ggtgatatct
      361 taagtaaaaa atgctattaa taaatacaac aaccttccca tacacagtta aaaactattg
      421 aaatcttgcc attaagtaac atcattaaca gacaaatatt taaatattta ttttccttca
      481 gaaaatgtga accatatatt gtggagcaca atatagcaat tagtaaaaac tagacacttt
      541 aacatattaa aagcttcacc tcaaaaatat aacaaaaatc tgatcaaaat tataaaaatc
      601 aattatacat tccaattaaa aatataccaa taaacagcta aatacattgt tgacctaata
      661 attacaatgc aatcaatcac agtagcttaa aataaacaaa ataagcaaat gagcacaaaa
      721 cctttaatgt tttaaaaata acttcaatta tttatattag tacaaatagt ttgatttctt
      781 ttctttttta caaacaccag catgaaaagg attacaataa cagtagaaaa tgactgtgaa
      841 aaacatatta acatcttctt tacttaaatg tctgcaatta aaaatggcat taaagaatta
      901 cattgcaaag agtctttatc tggaaaaagg ttcaaaagaa gtattgcaca taacaacttg
      961 aagttaactt gcaacattta ccacattcaa gtctttaagc tccccttcct ccagataggt
     1021 ctacataaga tcacctcgac agacagcggg gatgagttct caagtctttt aaacagtgtt
     1081 ctataaggat gctcaaagtc ctgcactgcc aaatcgattt taaagttagt cttgtttctc
     1141 gtcttatctc tctgtcatgt gcatcctgca gaggtctgcg aatgggggaa gcgaggagat
     1201 caacaagtgg gggcggggat ggggaagtgg gggtgaggtg aggtgagagg gtttggggtg
     1261 tccttaacaa cttaatagtt gcgaaacatt tggtgcacac cacaacaatt aattttaata
     1321 gctaattcat gtacttgtca tgttctcttt ttttgtcatg catgctaagg attacaatag
     1381 gtgctagttg gcaagttcaa gaatatata
//
LOCUS       KCVB01000435            1844 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20241pri ultra-conserved element locus
            uce-1030, sequence.
ACCESSION   KCVB01000435
VERSION     KCVB01000435.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20241pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1844
                     /note="ultra-conserved element locus uce-1030"
BASE COUNT          518 a          381 c          425 g          520 t
ORIGIN      
        1 atagatggat atatacataa acagacagcc cttatggtac gctgtcattt aatcctttaa
       61 ttgctatctt tgaaataatt gaggaatata ttgtaagcaa cacttaagca aaatggtgga
      121 aaaattaaat gcaagttttt ctgggatgag cgttccagtt ggaacttttc cattcacagc
      181 cgtgtcaaaa acaagttgaa aacaacaggc ttcctcctgg gcttttttgg gaagggagat
      241 ggggtggaag ggttgcaggg ggagcagcat agtggggttt taagtgaggg ggggacaagc
      301 agctgcccta ttagcccaaa tctgttgttc agaggaggtg ggaggagggt ggagcaagac
      361 cactgaattt ccacaaagga aagcacaaac tgttttcttc actttgtgtg caaagaagcc
      421 tatattctca gattatacca caaattaacg tccgtttaac ctctgacaca tcttcgggtg
      481 tagatcttgt cttattttta cccacaatcc aatgcaaaga acagaagaga ccatgggaac
      541 cccattaact ggcagccagt ctgattggaa ggcgtttttt tttttttttt ttaaaggctt
      601 ttctccaacc ttccctggga ataataagag aggtcagaaa gttgggtgac ttcctgctgc
      661 aaaaggcagg gtcagagcgc tggttggcag aagtagtcag cactcagcgg acatgacttg
      721 gaacacacat tcaaaaacac aaacacacac acacacaccg aacaagacag caaaccatag
      781 agtagaactt gtgtgattta cacacactaa gagcagaagc tggaaagact tcaataaata
      841 taatgaatga aggcgctaca gtgattatga gcctgtttcc aacactgacc tctacccaca
      901 gccacataca cacagattta tagtcacaaa catccaccga tatgcacgca cacacgcaca
      961 aacactcaca cattcacatc cataaatagg caactgattt acaaatgagc tgacctgtgt
     1021 gttggccttg ctctcagata ggctcacacc cccgccagga tgcataaatc acaatggaca
     1081 tgcatatgca tgagcatctg aggaggtggg ataagtgtgc gtctgcatgt gtgtgcgtgt
     1141 gcgtgtgtgt gtgtgtgtga gcaccagcca gcagcagtgt ttgtatcgat cattgaggct
     1201 tagttaagtg ccttttattt cattgagtta aagttaagga ccgtggacag gcctctctcc
     1261 cgtgcgtgtt gcttttgaat ggagagaaag caagacagaa agaaaaggga agagaaagaa
     1321 ctcgtaactg ttcttttttc cccatctttt tagtggcatc cagggatagc agatcacatt
     1381 aatggaacct agatgacgtt tcatgtctct tctctctgtg gaagaagtgg ttttggtgtt
     1441 ggacctttgc ctgatgtgtg tatgtatgat gtgagctgct ttaacctcct cttgttttat
     1501 ggacttatac atggctctgg atctcactgc tgctagatta atttacacac cactcacaca
     1561 tgcttgccaa atctccctct gtctccctct cttcctccct ctctctttct ctctgaagac
     1621 agagcagaaa ttgcctggtg aggtaaataa attacttcca tcgattgctg tagttgaaga
     1681 gtgactttat cacagtttga cttgtccaga tacagatcag cgtgtgacag catgtgtttt
     1741 ttataatgtg ccactgtgtc cacagtatgt gtgtgtgtgt ttgtgtgact gcgtttacat
     1801 gtgtgtgggc tgcagaaatc agtgtaaaat ctacctgatg ggta
//
LOCUS       KCVB01000436            1431 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20318pri ultra-conserved element locus
            uce-43, sequence.
ACCESSION   KCVB01000436
VERSION     KCVB01000436.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20318pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1431
                     /note="ultra-conserved element locus uce-43"
BASE COUNT          457 a          293 c          280 g          401 t
ORIGIN      
        1 tgacagctat actcaaaagc ctccgagtta atcctgcacc ctcattgcaa gaattaaaag
       61 aaatataata aacaagtcaa ttccctccct tgtcactatt tccagtgtga cataaattac
      121 cagaggaaag acaagctgag ttttacagca tcaggaaaat cttaaaacac ttacaagtta
      181 cactctttta cactcttgat attttgacag agcagataat acagtacttg ttgtaaaggg
      241 gcagaaacta tactcgcagc ttcttaatag gtcagccaaa ctgccactgc atgtttttca
      301 ccccgtactt aaaattacaa catacaataa taaggtagaa aaatattgtc gtaacaaatg
      361 tttgagagga tgtcgtgtgc tactttgtca ttagtggaga agctgtctgg taaaatcagg
      421 cataaacatg tgtcagaatt acatgtaatt atagttggaa agacttccgc atgggagtat
      481 gaatcacctt tgcagctggt tggcgtggca ggagagaata aagtaccctg ctatcctcac
      541 cttcgtaact gcatttgggg gaagggaaca gaacatggga gacatacaga ctaaagaaga
      601 tagacaggct catggtatgc cagagagccc aagttgctgg tcctcttctg gattatctga
      661 agagccaccg acggatatgt gtaatcagcc ccatccccat gtttctctct ctcactctct
      721 gcctctctgg ctaatgtctc cgcctgattc gattgaggca attaggcagt aatctataga
      781 gctagggagt gatggaaaca tttcccactg tcctcctcat gtttctcaga gctgctataa
      841 ttagctaact ttgcttctca ttaaacatca gagcagtatt tacataccca gatccaattt
      901 ggctctgtag agcccattcc ttttaaactg gcttgaaacc ctgaaacatg tctgtaaaca
      961 aaggaatgag ggagctgtaa tctgcagtta tttcaatcaa acatttaaat ataatattgg
     1021 taagacacaa tgtatctaca atgtacacat taaaaacaat gtcagagttt gtgtacatta
     1081 ctaagtagat gatcattcca gagcatagga aggcctgtta cggcatgaag tttgatctta
     1141 aaaatggagg acatcctccc atccctggtt tcaatgagcc gtcatgggaa ctggaatggc
     1201 aggcccactt atattcccct atggctcata ttcagcttaa atcgtttttg cagagaacaa
     1261 gcagaatcgt tatcacacag aaccgtggag aacgttaaac tgaaatgcag atggagagat
     1321 cttgactatc tctttcagga aagtatgact aaaggtttga cattctgtaa ctctgatctg
     1381 aacaaaaagt aaaagaaaac aaaaatatgc tgtaattctt catctcatct g
//
LOCUS       KCVB01000437            1634 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20359pri ultra-conserved element locus
            uce-804, sequence.
ACCESSION   KCVB01000437
VERSION     KCVB01000437.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20359pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1634
                     /note="ultra-conserved element locus uce-804"
BASE COUNT          517 a          268 c          351 g          498 t
ORIGIN      
        1 attatagggc taaatatgac atgttgtgtt ttgtccttaa gtacttaaga acataaagtg
       61 ttataactaa tgttattcat ttggttttgt gtatatgttt ctggtggatg tcttgctata
      121 tttttcatgt ctgatgtctc acagttccat gtggaaatga cacgcaaaaa aacccaaaaa
      181 ctaagtaacc aaattaagtg atttttctca gatggcatca ttttggctct catcaaaagg
      241 gactcacata gtgatatata catcctaaga tgttgtatgt taaaagtata cgacaaagac
      301 aatatttttt gttctgaaaa tatttcacat ttttatctag tttctagtta acagtaaaga
      361 aactggctat aagatcttaa cccattcttc tataaggtgc tcattttata gtggtgggaa
      421 cagttgataa gcagatgacg tttcacagta ggccaccgcg gcattagttc cttaagtgca
      481 ttctgcttta gacataattt taggcagtta accctctttg tataaggctg cataagatgc
      541 tggtcaattg cctcaactcc acttgtgaca aaccaattgt ttaaaaatct tgattttgaa
      601 tgctgttgat ggacaccaca aaggaaggag cacactgagc atggcggcct ctgtgctagt
      661 aactggcccg cagcactaaa gaacagtaat ggttctaatt ccaataaaga gaaaatccaa
      721 ggtcacactg tgaacttcct ccccatcaga agaccacaga gactggctgg gagagtgcat
      781 aatatcctca cagctatttt taccctcaaa ttaaaatcta tgacatgttg aaatgactgc
      841 agtgtgcaag aggggtattc aaagcagaca ctaatgacgc ttattaatgg cgtgaatgaa
      901 gtcttgacag gtcgtgtgtc attctgcagg caatgctcca ctggcataat ggctaatgta
      961 agtggggata attatatata ttataccctg tcttatggta ttttggcagg agtatgagga
     1021 gccaggcagt tagagaggag agtgaaagcg cttgctcttt ttgagacaga gatgtgcatg
     1081 cgtgtgtttg tgtgtgtgtg tgtgtgtgca cacgcatgta tgtgttcacc cccatcttta
     1141 gtagtggtcc agcggatgaa taatagtaag acaatagcac caaacagtgg ttgaataaga
     1201 tgcctgcata aagagacaga catcagataa gcatcattca caacatctgt ttctgtgttt
     1261 aaacgtttgg ttccggagat aaaacaaatg tggctttctt tgacagaaca aatgtaccgc
     1321 acattgggaa agtgattaag tatgcacatg tgtgtgatgt attgtggttt gctcgttttg
     1381 tcgcaggcag gagaggcaag caatggccat taaatccatg taagaggacc tgagctttga
     1441 ggaagagcct cacagatcag aagagagaat taggagagaa gacaaaaaaa tgaagacatt
     1501 cataggagtg aaaacaagaa aaaaaaagga ttataattat cttgattttt attttagtta
     1561 tgattacgaa ccctcttttt aaaaagaaga tgattttctt tttatttcaa aatcatttaa
     1621 gaaatattta aagg
//
LOCUS       KCVB01000438            1372 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20396pri ultra-conserved element locus
            uce-1088, sequence.
ACCESSION   KCVB01000438
VERSION     KCVB01000438.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20396pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1372
                     /note="ultra-conserved element locus uce-1088"
BASE COUNT          474 a          260 c          227 g          411 t
ORIGIN      
        1 attgattaat tgattttgaa aatagctgct tcccttttta ggactgtagt caaatttact
       61 caaaacaact tccacatccc aaagagctaa tgctaattca atcaaaagga tgatgtttaa
      121 ggattggttg tttgtagagg tctgaaatgg taaaacttgc cagttctaaa ggaaatattt
      181 tagatatctg aaaaataaaa atatccataa tttttttttg tagcaaaggt gctttttctt
      241 cctcgcctgt caataatttt tccaacgttc agatttgtca caacctcttg tagagatcct
      301 cacctccagg ttgggaatca ctggtttaat ctgtattcca ggaaaccagt attacaaatc
      361 tatagatagc attttggata agtaatgtat caagaaatca cttaactacc gtttaaaaga
      421 catcagtaaa gccacagaca aactttaaac tgaacatttt gtgggggtaa atccagtgaa
      481 agaagtgcga gttcatggca aaaggagtga agtcatttta tttgttttta atatgtatct
      541 acaacaaggg ggacacaggc aaaaacgact gacaacttga tgaaaattaa aacaaaacga
      601 aacaaaacaa aaaaaaaatt acatttatta tcaatgcagt tttcccacct ttttttccaa
      661 taagcaacat tttttcgtct aaagaaactt tacaatataa agaaacatac gaagaaggaa
      721 cctcttattt tactacacaa gacatttagg atctgaaact accctaacat gagatacaaa
      781 gcaactcccc aaggacagac tgcctcatat tcaatactgc tttgtcatca agaggaaaaa
      841 tgaggaactg aacagaaaag tctttactcc cctcaaaaaa ttcctctctt aaccctccca
      901 acccctgaat gttacagtat tatacatcag cacaatagaa atacagaaat gatcaacgtg
      961 tgacggcttt acttacagga cagaaagata gagagaagtt cgactgcatt ttatacaaca
     1021 gtatttcaaa gtacagggga ggaaacgtcg gttcccgcac tctctctacc tcttctcagc
     1081 aataagaaca aatctgtccg ttattttttt tctctttact ttcccaaaaa catttctccc
     1141 gccccatttt aaatagatta aaaataaaaa aaattaatcc ttcatagtaa agtctgttct
     1201 ctggtccctg gtgtgttgga ttgagagaca gtgtcctgtg tgtcctactt tcggttcgag
     1261 ttgtgtcgag agggtggaag ctgaaaagca agtgtatcgg aggcagaagt tttattttga
     1321 aaaactggcc tctttttgct ctgttcctgc aaattccctg tcaaaaaaaa aa
//
LOCUS       KCVB01000439            1622 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20442pri ultra-conserved element locus
            uce-303, sequence.
ACCESSION   KCVB01000439
VERSION     KCVB01000439.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20442pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1622
                     /note="ultra-conserved element locus uce-303"
BASE COUNT          427 a          376 c          360 g          459 t
ORIGIN      
        1 aaaaaaaaaa attaatgagt caagacagta atgggcagat tagttcataa tgcaaggaat
       61 atttagttgc aacactgctt tgaaattttg aatagtattt aattatcgtg tacaggcaaa
      121 gaactgctgt agcttggctc ttcttacatt aattagagca cttaattgca gtgataaaat
      181 tctatatttg ttacctttgg agctggagac ttcagtctga agtgactgca tagagttgta
      241 aatctactgc taatgcaacc atatcacagc ttttctagga aagtccgatt tgagtgaatg
      301 tttatatcag tgacagatcg tgtttgtgca cctgcaccac ggtcactttt tcattacttt
      361 gaaataaaat catttcctgt cccgtttatt ctgcctcgct ctgcccagcc ccgtaccatc
      421 ggctcggcgg gtctcagcgt ggcccagctc ggcttggaat gaaacggcgc gtccataatc
      481 tctctgccgg catgttaatt ttcaagtcaa aggggcagct cccttttcat cacggcggag
      541 gctgtggaat attcagcgcg ctgcagatgt tcatgaataa tgtctgtggc caaacccctg
      601 gctgaggctg ggggaggagg gatagagggg tgaggagggg aagggggtag agggggaggc
      661 agtgaggcca gatgaattac acgtaggggg gatctgacag gcggcgtgga gctttgtgat
      721 cattccaaag ttctaataat ctctctcacc ttctccatct ccccagtaat gaaagagaat
      781 aggtgattgc accccgggct atgttccccg cgtaatgact cctccaaagg cacagagccg
      841 ccaaggaaaa atgaactttg aatgagcggc gaattggctc tgacagagtg atagaccgtg
      901 acacagattc tgtagcaaaa cagacgtagt gctaattgat tatcaatatt ttaagcaaag
      961 tgatgaaaat aagcattaag agatgagaaa gaggagcgag aggcacaaca ctaccttcga
     1021 agaggggggc tgtcatttgt ccaatctccc tcactctttt tctctcccct gtgcttctac
     1081 gcctcctgcg gccaggctgc ctcacccgtc tcccccattt ccccctctcc ctggtgccac
     1141 tctcagcctg ccctctcacg ctctcattcc taccgtcttt gctgttttcc ttcatcattt
     1201 tagcgttaat tggcaaatat gtccctgaac ttgtattaat tttgtaacgc tttgccttat
     1261 ttatttcttc actctgtctc tgacgttcgg ctgaaccttg ttatcaggcc ggccttagtc
     1321 acctcgtccc tcagaatgtg taattttgga gttcaatcac ttattcattt tttctttaga
     1381 gagaaaagaa atcaaagact ttattcaaat acattgttgc caatgtcgag ccgtgtctct
     1441 acagaatttg tgaaaagata tcaaagaaaa cgaatacaca gtacttttca agaagtatta
     1501 catgcacccg cacacataca cacccataca ctcgacccac tatccgtgaa gtccacacca
     1561 aaaagcaggc aggggctagc cgagaccacc aagtttgtgt gtctattctg cagtgtgtgt
     1621 tt
//
LOCUS       KCVB01000440             857 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20524pri ultra-conserved element locus
            uce-797, sequence.
ACCESSION   KCVB01000440
VERSION     KCVB01000440.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 857)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20524pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>857
                     /note="ultra-conserved element locus uce-797"
BASE COUNT          244 a          192 c          164 g          257 t
ORIGIN      
        1 tttaaactag tgaaagtttt gaatgtgtgc attgtatcct tgagccgtca catcacactg
       61 gagcacgtgc ctaataacaa tgcccaacat ttaaattcaa tgtaaacgtt ttatttttat
      121 ttcataaatc ggcagttaaa cacctgtgga ttcaattttt ccataatttc tttgatactt
      181 catgagaaaa aaaatgaatg tctataaaaa ctatggaatt tttaaaatac tatgaagtat
      241 taccagctgg cctaattagg taaaaaggaa aagagaacgt aatcctgcag tacatattag
      301 tcaaattttc atctgggaaa acaaacgtgt caacaaatgt gctaatgccc agtaaaatta
      361 tttaaaaagc tacagttaat catctgcagt gcaagactta ttttttctgc ttcaaatcat
      421 tgaaacttgc aattccataa aaattctaat aatgaattca atcatctcca aggtattaac
      481 cagctaagcc gagaatgttt ttttcatata aaggagatgc tttgggagag ctcagggatg
      541 aatgtgtaat gagctcgaaa taacctaaag cccttttttc tctctttttc tcttctctgt
      601 gtcctccctc cctcctcccc tccctgtctt tttttctcct cttttgggaa acatgccttt
      661 cccagctgca cagcccccac agcgcgaggc caggccagga gcacagctgg ccagacttgg
      721 tggagccagt cgacaggcgc atggagcgca gcccgcccag ggcccgcttc ctccgcaaac
      781 atgcccaggt gtggggcctc cgagctgggc ttctgatctc caccaaacag tgctgtgtgt
      841 gtgtatgtgt gtgtgtg
//
LOCUS       KCVB01000441            1403 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20571pri ultra-conserved element locus
            uce-413, sequence.
ACCESSION   KCVB01000441
VERSION     KCVB01000441.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20571pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1403
                     /note="ultra-conserved element locus uce-413"
BASE COUNT          477 a          314 c          260 g          352 t
ORIGIN      
        1 caaaacaaaa gaaaaaaaaa ggtaaattaa ttgattaaaa agaagatgac aggttttgat
       61 ccaatatgta ttaagtacat aaaggctaag gaattgattt gtactattgt gaaacagatg
      121 gaatgatcac tttccatttt catttatcat tctgctccaa cctccaccct gtctttgact
      181 aagtgacaca ccctataaat cgaaatcttt gttctgataa ttgtttcatt tcaccggatc
      241 aattgtcata tccacattac acacacctca acagagttga tgtaataagg aagccaggga
      301 aatgttggcg cccagcaaca agtctattaa gccactagaa gtctaaacgt caacaagtcc
      361 aatcccactc agtgctaatc tgcattcatt actatgcaaa ccaaacaatc ctgcctcata
      421 tattctttca aagtcacctt tagattcaga cacttttctc atatagacgc tcgggcaagt
      481 cagaatcctc ctgagcacca aacaaacagc gcatcgtggc cgcatgtaga gacccacacc
      541 agtcagagaa cagaaaaggg aacatttcaa caaatagaga atagcatggc actgtcacat
      601 ggggagcaca aaacagcaaa taacagagac aaggtctgat ggtagccctt ggagcagctg
      661 tcatattaaa taataatgaa ggatctatta atatgcaaag gaagcagcag ccgactggct
      721 taacgctgtc atgcctctgc atattgtcag gatcttcatc attaattaac atgctagctg
      781 ccccatacct gcgccaatgt cagaacattc ttacaacaaa caccccaatg cctgtgccct
      841 ccgcaactaa ctcgtataat taaattaaaa tcagattaac ttcacatcag tacacaaaag
      901 actgactttt acgactttct ctagcagcga ttacagaaaa acagagctta tctgtggtgt
      961 ttctgctctg tctctgttag ggacggcacc atgggcaaca atgaaaacac actttattta
     1021 tcgggcaagc aggataagta ggcaagagac actccatgtc aatgagcatg gccgcctaat
     1081 tttatgcatg gactggtggc agcacagcaa tgacaaactg gggttgggaa caacgcgact
     1141 cttaggtaca ctgcaccact tgaactgtgc tatccacatc atgacaaatt tagtatcaat
     1201 tacacagcgc gctaacctta tgacaagcag tggactgaaa gaggaggggt agagacacaa
     1261 agaactgaca ctaaattacc tggtgcatac tggatctatt ttaaagcggc ttgaagtggt
     1321 accagattag atgatccact ctagaagctg acgtgctaat tgtacatcaa aagaacataa
     1381 atattacaat atgtctccac cta
//
LOCUS       KCVB01000442            1131 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20654pri ultra-conserved element locus
            uce-456, sequence.
ACCESSION   KCVB01000442
VERSION     KCVB01000442.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20654pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1131
                     /note="ultra-conserved element locus uce-456"
BASE COUNT          284 a          227 c          282 g          338 t
ORIGIN      
        1 atcaaagtag ttttgtcttt atacttccat atatttaata gctttatata aacaatctct
       61 ctccctttca ctaaaatgaa agtgaaataa agctgttgta ataagtttct ccccggctta
      121 ttaaaggctt gtatagctct tgtttaggat ttgcactcat taggttcttt ctttatgact
      181 tgttcccatt gtttcctcat ttcatttttg ttcctgtcag ttgtccgaaa agggtctaag
      241 caggggggtg atagatgcat agatagatag atggatgaat gaatgagtga gtgagtgaat
      301 acaagacaag gcagcagcgc tgaaccgtaa agaagtggaa aacaatatca gctgcgtgcg
      361 tctgtcagtt gcaaatgtgc tgtgctggcc tttgtttatg gttactggaa ggagacagag
      421 tcgtagagag agagacagag gagtatttaa acattttaca agactagaca aaggtcagaa
      481 tcaagttttt acacaaaacc tctacatata atctagaaat tctccgtttt cctgatagtt
      541 ttggtagaag tcggtcttgc ggttgcatga tttggtgagt aaactctttt ctgttttcca
      601 aaaacaaaat catcatccca ctgcatttag tacacccagc ttttttccca gagagagaga
      661 aaaagggggg agaaagagag acagagagac aaagttgcgt ggtgttggtg ttggtagaat
      721 gaaagaggag tctggtggca ggagtgtaga ggaggcagca tatggctgta gaaagagcac
      781 agctggaggt agcatctcca tctgaggatg atgtcagagc agctgacagg ctgccatcca
      841 ccccacctcc tcctcctcca tccctccacc gcctccaccc ctccccagtc ctttattgtg
      901 agtcgcagcg gcagcctggg agagttagtg ctctgtgtgt gtgagtgtgt gtgtgtgtgc
      961 acgcttgctg tgtatgtgtg tgtgtgtttg tggctgccga tcagcgccag ttaggaggag
     1021 gaggaggagg gaggtggtat ctcgttcccc ctccaacatc tcctccactt tgcatctgtc
     1081 cctcgcagtt tgaacagcgt gttttttttt tgcctgcctc cctcttcttc t
//
LOCUS       KCVB01000443            1517 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20698pri ultra-conserved element locus
            uce-712, sequence.
ACCESSION   KCVB01000443
VERSION     KCVB01000443.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20698pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1517
                     /note="ultra-conserved element locus uce-712"
BASE COUNT          464 a          320 c          290 g          443 t
ORIGIN      
        1 tccctcccac cctcccttca gtcaagagtg cacatctgct actttcctct cggtttgttc
       61 tctttgagca gccttgtttc accttgagga cacaatgggc tttccaaaac agtaagtggc
      121 atgaggaaca gatgtggaaa attcatagag acttgatagt tgcacttctg ctacagaatg
      181 cagttcatta atgacacaaa tattgactgg gcactgtgaa aggtaaatgg taaggctctt
      241 taagtagtca gggatgaccc tcaaaacatg tgtgtgaaaa taaggttatt aataaatagt
      301 aaaatacttg aaaatgaggg ctttcctctt gctagttatg tgcattggca cttaaagcca
      361 atacagttta acaaagctat ggatggcgct tcagggatgc tgaaggccac ttttgctctg
      421 tcccatcctt accagctgta tttgttctct tcaataaatt caaagtagcc ccttccatgt
      481 tcctcccggc gtctcttaac acccttctta ttcttctgat cagcattcgt gtctttgtcc
      541 gcatcccctt tgaaaataga acaaagatat agaatgaggt gctccttttt atgtgagaga
      601 gaggtcggac tggcagtggg gtgctagccc acaaagcccg ttcattagtt acattatgtc
      661 gaccataatc atcaatatta aaaaagtaac taagaggtaa cacctcatga ttacaatgaa
      721 acaacacaat catacttcat aaattgtagc agccttaatg accagatagt agttataaag
      781 taagctggtc caaaggaaca ttgtgaacag tgtttgctgg ggctggaaag ataggtttca
      841 gcaaagtaaa gggacatttg ccctggaata aagggcttag aatactattt tcaataagat
      901 tcagatctaa atatatttca ttaagaggac tagtctactg tggtctggga caaaagcatc
      961 atgcaaggaa atggaagacc tacataaacc tgcactgaac ccctcccccc accttacccc
     1021 aattgacctg ggtggctatt tattctatac ccaaattttc taggttcatt caatcaaaat
     1081 gacattttct attcaaacca cgtacaaact aaccacgtgc tacacaagca ggtcagcgcc
     1141 tgtcctatat tccacccagc tttgggtcag ctagttcagg tgttcccagt ccttttgagg
     1201 tcaagtccac tgtgcaaatg tagtcttgcc attcccaagg aatacgaaat atgagttaaa
     1261 tttgtattag atttgtaact ggccaaatgt ctcttccgaa tttagggtaa ataacaggat
     1321 aaaagaaaga ggaaacactt tatgataaat acaaaactta gaccttttaa acatttcaat
     1381 ggtattactt tcacggtgtc ttattttttt acatgtagat tataacgaga ttttttggcg
     1441 aaacgccact ctacctccac aatcagtaaa tcgtcaatat gggaacaact ggactagcta
     1501 accggctgat cagctag
//
LOCUS       KCVB01000444            1709 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20768pri ultra-conserved element locus
            uce-757, sequence.
ACCESSION   KCVB01000444
VERSION     KCVB01000444.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20768pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1709
                     /note="ultra-conserved element locus uce-757"
BASE COUNT          519 a          347 c          335 g          508 t
ORIGIN      
        1 tgccaagaag taaagaaaac agattgtgtc tgaagagcat tggaagtgcc aatgacccct
       61 ggtcacgaca gcaacataag gactgccaga gcagcagata aagagccaga cgggtcaact
      121 actctgtcct gccatgattg ctagcaccaa cattttctta aagggagaca aatttagaat
      181 tctgtgtagc ctcttgcatt ttaaagaaca ggatctttta gaataaattc cactattttt
      241 gctataaacc cactttggct ggatttgaaa taattttaac acttaatctc cgtcactgtc
      301 acgcattaat cctgtcactc tccactcagt gccctctaat aaattacaaa tacattaatg
      361 agtcttctag taagtcatat atcactctaa tactgttatt gagcatccca acctgtaaat
      421 ttgagatatt attgaaatag gaaccaatat ttaactcagc gttctgtaat ctctaactta
      481 taaatgtacc aagcactgta gcattaaaaa aatccaaaaa attgtatgat cggaaagaaa
      541 cctaattgcc tttcaaacct ctgcggcacg ttctatttat taatctgaaa gtgcttccaa
      601 atcttgggga taatgaaaat ctcttctata aatatgtatg cagtgaagtc acaacataat
      661 aggggcctgg tgaaaaacat gaggcagcaa ggcggctgaa ggatgaggcg caggccctgc
      721 agaaaagaat gttcccctag cagaaattgg tttaattaat aggacatttt acataatgat
      781 tacagtgtaa ttgcaccgac tagcagagtg tcattaaata tatccttgct cctttacaac
      841 accctctagc taaaaataat gagttacatt ggaaatcact atgcaacagg ttcataaata
      901 acatttaagc ccatacaagg caagtactta taaataataa atatttgcct tttgtaattg
      961 aagaaaaaat gactgcctct tttttttccg ccactgcacg ctgctttgtc ctgttgtagc
     1021 catggaaagg agatttttcc catttttttc ccctcttgta agtgctgaat aatttatttg
     1081 ccggctaata gtcaacactg tcaacacccg gtggttaaaa tatacctgtg tccagataaa
     1141 tgcagcagca ggctacaaag cgattattcc ctgctcccag atattttgtt ggatgcagtc
     1201 catgtataca ctgcagtaaa tcaccaactt ccaattacct tcctccataa aacaaggctc
     1261 gtattcaaat caggccccaa aagagaaaga cagagccggg gacaactgaa cccatgcctg
     1321 ggggcgctga ctgaatgtgt acatgcgtga gtgtgtgtct gtgtctgtgc ctgtgtctgt
     1381 gtctgtgtgg agggggtgag gggggacaat ttgtttcttc ctgtaatgct tagtcctttt
     1441 tactgcaaag tggcactgct ccactatccc tgcaatagtg cgtttatgct gcctgggttt
     1501 tcattggctt aaaaagttgg caactgaaat atttgagtta gtctttcttc aactgaggag
     1561 ccaaaagttt ctcttgaatt tcgtccaaaa caggatgaaa gagttcgata tgccatgatc
     1621 aagtgccatg attgcagaat taccaaagcg gtggtaattg tcctcagggg attgttgtgc
     1681 tgtaagtgga tctatccatc atttttatg
//
LOCUS       KCVB01000445            1266 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20793pri ultra-conserved element locus
            uce-839, sequence.
ACCESSION   KCVB01000445
VERSION     KCVB01000445.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20793pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1266
                     /note="ultra-conserved element locus uce-839"
BASE COUNT          320 a          263 c          357 g          326 t
ORIGIN      
        1 cagaccagca gagtcacgtg acggacaaag gctctctaat taacagagag gactctggaa
       61 agccgctctg taatgaggca ccatgctgga cgggcctcca gtgaggcact cggcccatat
      121 gcaaggccag cattgcagga atttacatac tgcaggtcaa ttgaacatat gcacactcaa
      181 aactgactaa tgacttccac tagcgctctg attctatttc acagcccgct ctcaggatga
      241 gcatagtata gaacactaat gaggataaca ctgggaggcg tacaactgca aacattaata
      301 tatgtatcga gacatgggtt caatattaaa cctggttgct ggggaaatgg gtacaaccac
      361 aacatttcca cgggtacaac ttcaagctgc acgactaaag ctcccataca aaggaagagc
      421 agcgttatat agtgtggctg atcagcaggg agcaaaagcc aatgtccagt aaagtgccca
      481 tccatgtctt tttgccgcac atttactctc agacagctga tgcccagtac ctgtaaggcc
      541 acccattcac tattaaactc ttcccccagc accccacagt ttggacagtt tgggggctcg
      601 gcaggggtgt gtgtccatct gtgtgtggct gcatgcttgt gtgcttcact gctcgcgtgt
      661 gcgtgcaagc gtgcagcctg catgcatgtg tatgtatgtg catgagagag ggggtttatg
      721 ttgaaggggt ggcggcgagg agttggtggg tgttaaaaga aggagtgctg gtggtggtgg
      781 gggggtggct cttatttggc tgggtgtaaa aaaatgcccc caggtctcct gatggaagag
      841 acaggtatta gctctgagac ctccatagtc ccagcttgcc attagtttcc tctccagctg
      901 caccaggatc cggactatga atctgccatg gctggtcatt catcagtttc acagtccgca
      961 ccagcacaca cacacattcc aataacaaca ctgtgaaata gtgagtgagt gagaaagaga
     1021 cacagagaga aagatggagt gagagagagt aaaagtgtga gaaaaatatc ttgtcataca
     1081 gagactttta cttcagcaag tgtgtatggc cactacatac tgtacctttg actcaaatgg
     1141 ggggtacatt accagtccat ggagagtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     1201 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     1261 tgtgtg
//
LOCUS       KCVB01000446            1076 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20877pri ultra-conserved element locus
            uce-1155, sequence.
ACCESSION   KCVB01000446
VERSION     KCVB01000446.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20877pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1076
                     /note="ultra-conserved element locus uce-1155"
BASE COUNT          307 a          200 c          256 g          313 t
ORIGIN      
        1 ttaatgctct caacttctct ataaaatcaa tgtaacactc tccagtggaa atctgggttg
       61 attgtttcac ttggttttgt gagttcatta tgtcattttt caggcttctg gggacaaatc
      121 atttaatttc gtagcctgtt ggtgacgaca ccgaaaaaca acagatgata tttcagagag
      181 tgaggaaggc aaatgtaatt aaacaacaca taaataaaac tttaataaat tgctattgtt
      241 ttcaaagaga ggccctgagc agcagaaatt acatactgtg cctttaactg ggctgttgta
      301 ctcttgagaa gcagtaaaga gcttggaggt acatgaaaaa gccaaggaaa cttgccattt
      361 gcagaagact ttggagtttt catctttaaa gtaccaatat tttgcaggcc aaaacctttc
      421 acggaaacta gcactgtaca tataaatgtt tgggacaatt gcacagagga ggtggaacca
      481 aaaagcagct ttaaagtccg tcctgtttga gtttcgtggc atgtgatgaa ctgggaggag
      541 attctgctct ttgaagtgca tcagttgtgt ctgagcactt tgttatgcac agtggctgca
      601 gggaggaccc caggagcaga cccctgcttc cctcatttca tttactctca ggaaaggtga
      661 agggtaaagc cactggcatg atgaaacgct gctacccaac tctcattgtt tttaaggggc
      721 gcctgtatgt gttcacgctt ctctttaact ctgagcgcag ggggaaatta taattaatct
      781 gttaatgaca gtcggggctg tcataagctc ggggagtgta taataaattg ctgtaataaa
      841 aattaagatt gcatgcatca ttgaggcctt cagagggttt gtttaatgta gttacaaaaa
      901 tggtagcagc tgatgtggcg tccactgctc taccaggaga acgctaattt ctgagtgaca
      961 cttaacctgc tgtagctcaa ccacggctga gggaggtgag gggaaaccta atgagttttt
     1021 cctgcgtccc attttgagaa tcactgaact ggatggcctg gtttgtatcc gaaagg
//
LOCUS       KCVB01000447            1106 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 20998pri ultra-conserved element locus
            uce-961, sequence.
ACCESSION   KCVB01000447
VERSION     KCVB01000447.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="20998pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1106
                     /note="ultra-conserved element locus uce-961"
BASE COUNT          334 a          252 c          275 g          245 t
ORIGIN      
        1 gtttccatca cattgataca acaagttgca tgtctgtaag agtaaccaaa aaaaaccaac
       61 tcattaatat agggcagcat tgtttgtttg agcaatgtaa ggatttctca cacccgctct
      121 ctttggttgt aacctacttc acccacagga cagcaaaaat tgatacactg gagacagata
      181 taccagcgat gacaacatga gtgaaaagca agcaacacag agggaaaaag ggacagcatc
      241 atccagagga actcatcagt ttatggcagc cacaggcctt gtgacctgtt acatttttga
      301 ctctaaacct gacaattaag cagcacaact atacagatat ataaaaacca gattagattg
      361 atgatgagtt gccctggaag agaacaggta acagacaaaa cggcagacaa ggcttgtaag
      421 agaggcaggc tgcatcgagg ctgtctaaaa gcgtatgtcg tgtgaggggt cttcccacag
      481 gcacagctca tcagcgatga ggcaaaggaa caagagcaaa gaggggagaa ggatggttgg
      541 tgacacaagg aggaagacgg gagggacagg gttttttttt tcctttttgt tttagtttgg
      601 catgtggcgg cactcaccga ctccaggccc ggtgacagag ctggctggct tgttttgtgc
      661 agaagcggca gcggccacgg ctgtgccgta tccgccctta cagaaatccc cactccctga
      721 agcctggccc acatcctcat agcctgcaac agtcagacac acagccgcag cccacagcat
      781 tagacacacc aggcagggga agcagacctg cctcagatag catttgcaaa caattcatag
      841 cgtttgtttg gaaccgtgca ccacatggac aggttttacc aaactgtgac aatacaaata
      901 gtgtcaagtc ctctaagaaa ctttactgac atccaaatac tttgctgtca ttatctgaac
      961 tttgacacca agtgcattgt tctgtgtggc aaaacctgtc catccggtac aacaagcagg
     1021 tcagattcaa cgctgacatc atttgcaagt actatttgac ccaagtctga ggggaggagg
     1081 tgggggaagt gagggggcag gcaggg
//
LOCUS       KCVB01000448            1879 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21040pri ultra-conserved element locus
            uce-1211, sequence.
ACCESSION   KCVB01000448
VERSION     KCVB01000448.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21040pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1879
                     /note="ultra-conserved element locus uce-1211"
BASE COUNT          456 a          520 c          380 g          523 t
ORIGIN      
        1 tgtttaagtg gaatgcaatg atcagaaaat gtttcatcat gatcagggtg aaagggaaag
       61 cagacttcac agcctgttag gattttaaat cttcactctc tctcttttgt tgcctctttt
      121 tcctccagca tgagcataaa actgaagctg ggtcgatcca ctcgctccat ctatctgggc
      181 ctggcctgtc tgtccctcgc tctccacctc ctcctggcat ttttttgtct ctctgtcctc
      241 cagacagcct gtgtccttcc cacctcctcc tcttcctcct cttcctccct taccgctccc
      301 ctgtctcatg ctagccaacg aagactccct ttccaaggta tgtcatacag actgtttgtt
      361 tttgttttct tcccccctct ccatctttct tttttctttg ttttccgtct ttgttgtctg
      421 tctcatttca cccaggtcgc ccacccaaat acagcagcgt cccggagctg ggcagcctca
      481 ccccgacctc cccctccagc cccgtccatc ccctccccct gcccagcccc agctctgggg
      541 atgtaagtaa tgatggggga gacggacagt tcagagggtt ggaaaccccg tcacacacaa
      601 tcctattggc ccagtagctc tgtcagtcat ttacacccca agacctccac aaacacgtcc
      661 attagtttgt tgtctccatg tgattatcat cagtcatcag ttgattttta cattgattca
      721 gccttacaaa gtgaacagtg tcctgtagcc caaagcatcc agtaataact actactcaga
      781 acacattcta actcccaaat gtcaagtaac ttttctgtgg ttaattaaca cgccatgtcc
      841 attagacatt aaaacaaggc caactgtaaa ctccaccaaa ccagatcacg gtgcctcccc
      901 ctgcctctcc acagatggtt ggtggggctc agctgggcag ggaaactgat tggtccaatt
      961 cttcctcaca gggagacatc catgaggatt tctgcactgt gtgcagacgc agtggccagt
     1021 tgctcatgtg cgacacgtgt tctcgtgttt atcatctgga ctgcctggat ccacccctga
     1081 aaaccattcc taaaggcatg tggatctgtc caaaatgtca agatcaggta actgcagcag
     1141 ccgtgactga tcctgagtca gggggaaggg ggtaccgcag tccagagagg ggccggtcag
     1201 ggccagtagc tgtacttgac tgtaaaaact gcttttgctg acactgtcct gaacattagg
     1261 actgttcaat cctttgtaag cataaacatg atgcagtatt gtttttttgt gcttctgtcc
     1321 cattcattgt gtcagtaaag attggcatct cacatccatc cactccattg cttgtccctc
     1381 ttcagcccta ccacagggtg gtggctaaat ttgtcttcct caaatctttt ctaagccaac
     1441 gcatcaatga caactctgag cttctctctg cagatcctga aaaaagaaga agccattccc
     1501 tggcctggta ctctggctat tgttcattcc tacatcgctt acaaagaagg tgacattaga
     1561 agatcttctt ctctccaaca tctgctgcaa caaatgtgga cagttcagct gtgagattaa
     1621 aagttctgtt ttattgttct tcgctgcagc aaaagaagaa gagaaacaga agctgatgaa
     1681 atggagttct gaactgaaac tggagcgaga gcagctggaa cagagagtca aacaactcag
     1741 caactccata acggtaagaa cacaaggagc tgcttccatt ttagaccggt ctcaggctca
     1801 gaaaataccc agatacactg aggcaaagcg ctcttctcag tctcaaatgt gaagtgatgc
     1861 attcgctggc tgacgaaac
//
LOCUS       KCVB01000449            1765 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21087pri ultra-conserved element locus
            uce-858, sequence.
ACCESSION   KCVB01000449
VERSION     KCVB01000449.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21087pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1765
                     /note="ultra-conserved element locus uce-858"
BASE COUNT          586 a          351 c          325 g          503 t
ORIGIN      
        1 tgaatcagtt gttactgtcc cttagctcta ttcaagctgt cacagtctat gaaagaataa
       61 atgaggtatg ggaacataag caagagcatt gtgaaagcaa tcatcactgg ccataaaaac
      121 tatctcttga aaggggcttt aaggggcagt gatttgatgc ttacacagtt ccaaatatat
      181 agattaaaaa taaacatata aactccatac acacgttgga tcaattcatc tgcaaatcta
      241 aagagatgaa acagttaaca gcagagttgt tgtggcaaga atgaagcaag atggcaacag
      301 aatcttatta aaaaggatgg aatatggcac cacaaaatta cttaatattt ctctgtgcaa
      361 cttttgcaaa atcacttaaa aaatgaagac ataaattcat aatgtgagct tatttgtctt
      421 ttatgaaaca tctatttggt taaacgcatg ttaacactaa aaaaattaaa acttcaagcg
      481 tggaatcttt gacctaatga aatgacatac tgtgccgtac atgtactata tgaacataca
      541 ttaaaaatta agggatctgc attcacatta ggtgatgctg taggtaattc atgtcaggat
      601 ccaaaacctc aactgcatgg aacccaacct gtcgatcaca ggatttatgg ttaaagggag
      661 gaatgggcaa tgggtcccag tgaaaaatcc ctctggcagt gcctcatgga aacgccccaa
      721 acgctgtacg gaatcaatca tatcagggat tattaagaga cctgagaaca aggtcaaaga
      781 cttgcagaag aacacagagc aagttaaact taacttccac ttcatccccg aggctctgaa
      841 aatacttagg agatagttga ggcaggctct gttgaaagct cccccgccgg cacgcaaagc
      901 tttacttttg atgttaattc cttgaaagct gtttctttag ctggggatta gtgcatgaag
      961 agctaggctt accatgctct ggctaataaa gatgaaacat ctaacacagg tactcgtaca
     1021 tacgctcgcc tgggaacagt gcctataaag ttgccttaat tatagggaac ttacttcgct
     1081 tcagtaagta taagaatagg acgacatttt ttactttgaa aatactgtgg tattaaatgt
     1141 tgaaaatcct ttgtattctt tagacacaag tcttgaagta aaataatgag tatataggca
     1201 catcattttc tcagctcatg cgatcaaagc tgcctgtatt tcagagtacg ctcagagcct
     1261 ccacagctta ggtctcctta ccataccgca aaagactgac acttatgaag tgtgtccatt
     1321 acagctgctg attagcctcc atgtctgttc tggcttgagc gactgttgta gctaatgatt
     1381 ctgtggcaac gtgcatagaa aaccgacacc acaattcatc tgctcagcta aaatctccat
     1441 gattaatagg tgtttcacaa gaacattcgc ccctttgaat atttgaaatg gttctcccca
     1501 tcactgagtt ctctgctttg acagtttaag aagtaaatta atcaatccaa tcactgcatc
     1561 catcctgctt tcgagtacaa tgaatggctt tcctttcatc acttacttgg ccattttcca
     1621 ataactaaca aaagggtaat taattccagt gacctttgtt tactaaaagt acaggtatta
     1681 tcgaacatca cagtacacat attgacaaga caatatcagc taattacctc aataagcatt
     1741 ctaacctgca cggaaaatta aatat
//
LOCUS       KCVB01000450            1395 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21128pri ultra-conserved element locus
            uce-736, sequence.
ACCESSION   KCVB01000450
VERSION     KCVB01000450.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21128pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1395
                     /note="ultra-conserved element locus uce-736"
BASE COUNT          320 a          362 c          395 g          318 t
ORIGIN      
        1 atttagtatt tggtctttac acttgaataa acgtaaaaac agccctaaat ttggtaggaa
       61 gctatgtctt aaaacatatt tgctcaaact tgggtgaaaa cactgtgcat catagctgag
      121 gatattgtaa actgacactt gaatgtaatg attagcagga tgctcgcgtt atcttggccc
      181 tgtgagcagg tgagatgaca gaaaaccttt cgacagcctc tcaggtttgt tagaaaggtg
      241 tgacatcagc ccgtgaaggt gcgaggagaa tgaaacctgg ctgataatta ctgagccggt
      301 aagagctgtc tgtctctagg ggctgtaggg tctttctctc tctttctctc agtccgtatc
      361 aatctgtctc tggtgcgtga gcgcgcgccg catttctgat cggcgcgcga atgatgatga
      421 tgatgagagc gtgacctcac tgctttctcc acagctgctg gagagagggg gagacgcaca
      481 cacacaggca gagacagcga gaaacagaaa acacgttgag ggagacagag agaggtacag
      541 acccgcggag agcattcaca acacgggggt gacagctcgg cgcgcgggga ctcggtcgtg
      601 gggcgtccgt tggctgcctc ccagaatgtt ctgtgccccc ggttcatttc tacggtacac
      661 gaaccagtcg ccgcggcgtc cccacggaca ctgaagccca gatgctcacc ctctccgccg
      721 acatggacga gtcttcgtcg ctgctggatc tgctggagtg ctcggtgtgc ctggagcgcc
      781 tggacaccac ggccaaggta ctgccgtgcc agcatacctt ctgccgccgc tgcctggaaa
      841 acattgtcag ctcccggaac gagctccgtt gcccggagtg ccgcatcctg gtggactgcg
      901 gggtggacga cctgccggct aacatcctcc tggttcgcct gctggacggt atcaagcagc
      961 ggccccagcg agggagcgga ggaggagtag gagcaggcgg caggcagtcc ctggccgggg
     1021 gctcgctgca ggggtacgca gccgggatat ccgcttctcc cccggggagt gcgatcagag
     1081 agctgccagt agccaccagg agctcgcctg ttaaggttgg ttactttccc accatctaca
     1141 atatgtgctt gtgtctcttt ctgtggagga ccaaacaccc aaactatgtt taaatgtttc
     1201 cgcgtttaaa gaacccgtgt ttattcacaa aaacactctc tcttgaatat acgacttaaa
     1261 cattgtgcga atcctcagga gccccttgtg aattcggcat acagaaaaca caacttcaag
     1321 cttggttcct agtgcacgct gctgcccatt gtagcagcag aatttggtgg aagaagattt
     1381 cgtggaacat tgcac
//
LOCUS       KCVB01000451            1717 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21172pri ultra-conserved element locus
            uce-773, sequence.
ACCESSION   KCVB01000451
VERSION     KCVB01000451.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21172pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1717
                     /note="ultra-conserved element locus uce-773"
BASE COUNT          497 a          329 c          353 g          538 t
ORIGIN      
        1 tttttccaga gccttactga aaaggatctt tggaagtgct gtgtcttgga attgatatgg
       61 tggaggtgag gtaaaggggg aggctgaagg cttaatgcaa gcagttgaca tgaaaattaa
      121 ttgaaaaaaa aatatctaaa aaaacactaa ggaaggggtc acagaagaaa taaaaatatg
      181 accgctccta agctgcgttc tgaggtaaaa catttgctcc cagcgctgct cactcactgt
      241 tttgtcatgt ctaaatgtgt ccacatggtg acactcttga gctgtgagtt ttaaatccac
      301 tcctggctgt cggcctctca gcaattccat cacttaagtt gcagaaactg ttattcctct
      361 aaatctaaca aagacttcaa tcaatcatct ctaactctgc agaaattgtt aatttttctg
      421 cgccctgact gatggagtgt ttataacaaa aagtattttg tacaattttg cagtaagagc
      481 cgtacatggc ccgaaaccaa gctcactata taatgtggag attcatatga aagtatttca
      541 tctgtggaaa gtcatgaatt tctaagtgtt ttaagttttg catttgcaag gctttaattt
      601 atttaagtct attttttttt tcttttttgg tgactgaagc tgaagtaaag cactctatac
      661 ggtagcatgt acagtagagt agcatgttgg ccttccttct ccttcttggc tggcagggaa
      721 ccttcctgtt gtgatccgac ttctctcctt tgatcaaaga acagtgttaa agcactggga
      781 gaaggaggag gtatgaaata tgaatgaata cagctgtgtt ttgatggata tgtctcaaag
      841 tatcccagta agagactgct aaaggccagg aaatctgttt gtgttgtgga aataggctct
      901 tcctcgcctg tttcttcacc agcttcatct tgggtagctt ttcacttcat attttagaga
      961 tcctttgtta ggaaagagat caacccctta tccatgcatt tgtaggaaac tgttctaaga
     1021 ggccctagtt cataatatat atcattaatg acaccatttc ctttgtttgg tcttgaaacc
     1081 accttttgaa gaaaatgagc gccagccatg agacgaggat atttattatt tttctggggt
     1141 agacccaaag ttctcacaga tagaatgaga tcttatatga ggtgatggag gctttgtcac
     1201 tagttatgtc tgtgattcca cttccttttc gaaaaaactc cagcgggagc tgtgactcat
     1261 tttgaagttg gaagttgata ctgtggtgta aaactaacgt tcccatctga attaagaaac
     1321 tatcagagct cgcacaaatc tcagattttc agagtcagat gaactcatct ctcacattac
     1381 acttctttaa taaacaaaga taataaaacg ataggctcaa ttattaggag aggctgtaaa
     1441 atgaaaattg gtgcctatag atgtctttca tagctcaccg cagctccact gtcttattaa
     1501 taagcattta aagactctcc tcagtcattt aggactatat taactaagat ccagcctttg
     1561 gacctttggc aggacaatgt atctgaggtt caaaactttc tgcccaggct ttattagcct
     1621 cactaatagc tgggttcccc aaatgccagg gtccacctct gctgggagta agggctcaga
     1681 tcctgtcaga aaggggtcgc attaaccaac tgtaatc
//
LOCUS       KCVB01000452            1590 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21213pri ultra-conserved element locus
            uce-1091, sequence.
ACCESSION   KCVB01000452
VERSION     KCVB01000452.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21213pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1590
                     /note="ultra-conserved element locus uce-1091"
BASE COUNT          428 a          371 c          298 g          493 t
ORIGIN      
        1 aaattcagca ttaaatgtta cttttttcta aagatggaat attttaaata gatgtttgta
       61 catgttctgt cctgggttgt tggcgagcca aaattgcaca ttgattaaca attaaacaac
      121 cgagctgaga aaagagatta ttatgaaact taaactacaa aaatagatga gggacaaaaa
      181 caaaaggcat aacgcaatct ttcttacaga tcagtttttt tcacatcttt cctcgctcct
      241 catcccccaa acccttggga agcagcactc caaggtcttt catttgagcc tgtattatgc
      301 ccccgtatct ctgctcattt ttcatccgca ttgcattaag tattgatatt gatttcttgt
      361 aaatggtggc gaaatggatg gctcgcactt ttttgtgtta ttgttctgca gcacttgtag
      421 gtaatttatt atggctgagg gtccggtgag gcctcccagt tctgtgtacc tcctcctgat
      481 ggagtgtgcc aacctgacta acaaaacaca ccatgcactg aaaggccact cacaaggaga
      541 atgaagcatg aagagtgtgt gtatgcacac cagcaccagc gtgcacatac atgcacattg
      601 tgtcccatct cactttcacc ctcaacatcg ctcacattca tggtccacca taaaccggca
      661 cacataagag ggtccctcgg aaaacacata gcaataaact gaaaagtgct aactttacag
      721 gcatttctca atcttaacat gagagttatg cagtgggcaa ctattttatt tctcctttcc
      781 ttctattttt gaatattatt ctttttaggg ctgtagcagc acttgtgctg aaatccattc
      841 ccagttttgt gacaaagcgt ggccttcttg acaaactgga gtggtttact tgcattgtca
      901 tcctggccaa tttcttttcc ctgctgccgc ggccccagca gtctatttgc atttcaatag
      961 cctctctgtc tgcctctata ttgcctgata ttatgtctgc cttgtatgaa ttcgaattca
     1021 atgtctgcat ctgctgttcc actcctaagc agtcctcgta aaaggtgaaa catgaaataa
     1081 ggctcgcaag caaaagacat tgattgtgtg tgatgatgta aaccaagaca aactcaatct
     1141 gggggcttgt gtatggattt cctttaataa taaatttgtt tacatgcatg ttccgctttt
     1201 acatacaact gcattagaac aaccttacag ccttaaagtg attgtatgct tttcattata
     1261 gactgtgtga tttccttaat agaactatta cctacaggcc taccaataag ttttcacttt
     1321 tccatgttct ttcatagcta tagctgcaga gtaatgcaca catagtaaga tttgttctga
     1381 ggtaagcaaa ggcactgtgg taaaaccgat acgtcctgtt tacggtctct gtcaccacaa
     1441 caccagcact tggccacaaa gcttggctgg tgccacagtg tcggacacca tgggtcacat
     1501 gacccacacc agcgtctcac aagggtccct caggtatccc agaatgccct tttctctggc
     1561 tgactctctc tctcttgctc tctcatacgg
//
LOCUS       KCVB01000453            1616 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21256pri ultra-conserved element locus
            uce-437, sequence.
ACCESSION   KCVB01000453
VERSION     KCVB01000453.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21256pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1616
                     /note="ultra-conserved element locus uce-437"
BASE COUNT          519 a          321 c          300 g          476 t
ORIGIN      
        1 ccccttttgt acaactgata tgtaacatat caggattttt tattctaaca ttttagccca
       61 ctggattaat gggcaaagat gacataatgg agtgaaatca gcacactgtt taaagtattt
      121 caccgctttt caggacaccg agggaatatt atctctgaca aagctgcgat acctgcacaa
      181 accatccact gtcactgagt ctgaaagtgg aaacaattgt gttttatctt ccattcatca
      241 cacttccaat acacacaaag aacacataca atcatgtaca cacattgtaa gcacagagca
      301 tggacttcaa ttaaagtccc attaaagttt gggctgaaag gggggtgaac cataggaggc
      361 agagaggggc atttattgat ggcgccctca catctagttc ctgcattaag ttgattttaa
      421 aagcaattaa tacacatggt cagggaaaag taatggagcg ccctatgttc ttaacctgcc
      481 ggacacttcc cagccgaagc cctggccaac actgctctac tttgcattat aaatgacaag
      541 ataaatctta atggattgag cataatcagc tagccatgaa cgctgacata tacacacatt
      601 tactaggcgt actagtgctc acatacatat acacaaacat ggacgtacag acacaaggga
      661 aattaaaggc tttgaataag tctgcattga ttattttaaa ttattagccc acctctcttt
      721 ggacctccaa tgcttttagc ctaagcttta acacatttac agagacttat tgactataat
      781 tgcaactgag tcttggtaat ggacacatca attatgagtc gcacatgtct ctgaccaagc
      841 gttgttcagg gaatcccaat ctggggtcca ttagcgtctg ctctgctggt ctgaagctca
      901 atcagtcttt accttcacct ctactgaaac tgctattacc tggctgcagt agatagtagc
      961 cttaacatta gcttgagctg ccaaagaaaa ggctgtatgt tcccactcaa tactgctgta
     1021 ccttaaaaac aaaggcctaa tagctgagca gctgcacaaa gagattattg tttactcttt
     1081 gatgaatttt cggtaaagca tgatgtgttg aggtgctcat gaggcattaa aaggccttat
     1141 aagaaccaca aatctaatct gtatcactca caggaggata agtaatatac atatcttaca
     1201 actgtacagc tgcaaaattg ctttatacat aaatgtattt ttcatttttt ttattaaaat
     1261 attttctcta gtccaaataa gtttataaat tgcatcatat tgcatctaaa atgtgcatta
     1321 tcaattttac aaacccaaac atttgcaagt ggataagata taaagctaaa accttcctaa
     1381 tgctgtgttg tcatgtagtg ttttgacaaa gttgtagtga gttgtaaggt ttgcgcgcct
     1441 ttaatcttac ttcattcaac acttaaaatg agggataata gctttgacct gcttttacac
     1501 agagtaaata ccgcatcagt gcatattgta tatacagtat gtgtaagaga cacagaaagc
     1561 agaagtgaaa ggaggagcat gtgagtaaac tgaaacagga tacataccgg tgtgtg
//
LOCUS       KCVB01000454            1584 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21298pri ultra-conserved element locus
            uce-1328, sequence.
ACCESSION   KCVB01000454
VERSION     KCVB01000454.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21298pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1584
                     /note="ultra-conserved element locus uce-1328"
BASE COUNT          452 a          331 c          338 g          463 t
ORIGIN      
        1 ttcatttaat ttttgcagtt gtttaattat catacaatca cagattttct agtcaccata
       61 ctagttttgc atatttacat ttacagatat tttgcacaaa acctgaaggt gcagtttgac
      121 aaacaattaa gtaacacaga ttattttaaa ctaaaggtgc tgatagatat ttctaccact
      181 atataagtaa tttgaatgta tcattttcta tccagatttg gttatttgat tgcttcaaaa
      241 ctttaagaac ttcccacaca aatttacctg gacacatcat ataacatgag taaatcgaag
      301 tgtacacata tatggaattt gactgagatg taaaatgtct aatttggagt gaatgaatgc
      361 atcacatcca ttacagcaga ggtggagcac atgtggtttg tactaaaaag aacaacatct
      421 ggcctcagca tcagtgtgtc caccctgcag ttccctgtga ttaattcaac atctcaatca
      481 catccaatat ccacagctga ttttctctcc actgctcccc tcaaaatcac catgatcaaa
      541 aaatcccacc tctacctcag accacgggca ggtacttcct gtctgtaccc caccccccac
      601 cacccccaac ccccaccatc ccctccaccc tcagattgga ccagtcatat ggagggggta
      661 atatgggtat tgttctgggc tgggccggcc tcttcacagg aacattggat tctgtttgaa
      721 gtcgtgatcc ggcacggccg actcacctgc aagatcaata cgccgggttc aaagctgccg
      781 gcttcacaga gaacacactc ttattaattt atagaaactt ctagaaactt tgtctgaagg
      841 gaaaaacctc caactctgtg tggttggaga gacaggggga attattaata tacctggaga
      901 gagaaagaga gatggagtgg aggggaggtg ggggggggac catcttctaa ctccttatat
      961 gaatatttaa atgagttact gtttgatgtg gattgatcat acaaaaagtg tgtgtgtgtg
     1021 actgtgttgt gtgtgtaggg aggagacaga tggataagga agatggttaa aagactcatt
     1081 agatcaagaa cacaagtgtg tgtaggcgag tgagtgtgtg tgtgcgcaca tgtgtgttta
     1141 actcattaag ggagtctatt tgagttttcc tttgttgtca gatttgatga tttgggggaa
     1201 atttccagcc ttggattcaa agcattcctg ttcggcgctc caagcccgcg gcgtcaggga
     1261 atgaaacact cggcatttat atttaatgat atcaatttat caacaggaac aatctcagga
     1321 tgttggctca gatttgattc catgttcccc tgtttttacc tgagggacac atgcacacgt
     1381 tttctcatcg gtgcgccatt accatactcc tctgtttcac tcacatccgc gcgctgataa
     1441 tacccaacaa cctcctgaca gtgcagttca agctgtgcct cacttactca cagcaatgca
     1501 gaaactctga gactgtggtg ctgaagagat ggtgagattt taggtggagt ttacgtgtga
     1561 tatatgtagg cgagttaaaa aaat
//
LOCUS       KCVB01000455             958 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21332pri ultra-conserved element locus
            uce-242, sequence.
ACCESSION   KCVB01000455
VERSION     KCVB01000455.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21332pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>958
                     /note="ultra-conserved element locus uce-242"
BASE COUNT          271 a          261 c          184 g          242 t
ORIGIN      
        1 cacacacaca cacacacaca cactcatccc agtcttggcc tgttgatgga taatacagtc
       61 agtggtgtgc accatcacac acacaggtat tcagcactga cctttgcagt gcgctcccag
      121 cagtctgctt tggcttcact tgctctagcc tgtaaaacca cctcagtgac agatgatcag
      181 tgcacacttg tgcatgtttc tctctccaaa tcacacacag accaactgga aagctgagtg
      241 ctctgattag cctcatgaca cggcgaaatg accagctcgt gctttgtttc cgatcacaca
      301 caaaaaaaag acaaaaaata aaaaagagag gctcacagca gcagagatca ttaagactga
      361 tacctccagt agcgtttacc ctctctgctc catcttcttt ttaaaaccgc ccagctgatt
      421 tcgtctcctc tctaccccct ttgtagataa ttggaacaat cccactgatg cccaactctg
      481 cagggccctc cagccaatca gggggtggca acccagcact gcaggtacag ccaattacac
      541 ctcagcttct gaccaacgct caaggccaga tcatcgccac agtgatcggc aatcagatcc
      601 tgcctgtcat caacacccag ggaatcacgc tgtcaccaat caagcctgga cagcaggtaa
      661 catagacacc gatgcacaaa cacacacata tacaggtact taagctgata tttcaacaat
      721 gatcaagact ttaaaccgtt aaatgagcaa aacgtagtgg agacagactg gagctcaaag
      781 caaccagctc tgctagtcag gtttgtttta tgctaaacaa ctcatacttt cctgcttcag
      841 cactcataac actcatttgc atcattccac ttgaaaaatt gcagcttttg gtacgattgt
      901 tcacaatgtg tttgtgtgtg tgtgattgtg tgtttgtgtt tgtgtatgtg tgtgtgtg
//
LOCUS       KCVB01000456            1603 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21398pri ultra-conserved element locus
            uce-1235, sequence.
ACCESSION   KCVB01000456
VERSION     KCVB01000456.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21398pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1603
                     /note="ultra-conserved element locus uce-1235"
BASE COUNT          462 a          329 c          356 g          456 t
ORIGIN      
        1 ctaaatacaa gtgggtgtaa ctttgtttcc acacccttac tggagaatgg tgataataac
       61 atcatctgaa gacttgaaaa ctttggtgtc attttctcca gtaacggctc tgaaatggaa
      121 ccaaatctat aaaagcagga tgttttgcac atttcagcaa aagatagtgc tgtctaaata
      181 ttggcataag tggacctgta atgacaggca gcacattaaa tcagtaaaag cactagagag
      241 ggatttttat cttattatta tcttccttcc ctaaagacac agtctgtaag ctgaaactgg
      301 cctgaattct caagagaatt tgcaacgaga cgcatgttct ttaacaagtt cttaaagaat
      361 atttgtccat gtagcaagaa aagaaaagag ttaatcatac ctcctaataa ttaactccgc
      421 ctctgccctt aattcatagc atttctaagg gtctggttac tgccaattgg tagtcaattg
      481 tctgtttgga attcaagaca catgcgctat tgaatggtga aattgcagca agtctttctc
      541 cagcaaaggc cagaactggg cgccataatg atattcattt tcaaacagga gaacattcta
      601 atgggcaatt aagatgatca agcctgccaa gatacacgtg aagaacggag tgctggtgcc
      661 agtgattacg tgtcctccgc ccctgcctcc ttcctgcgct ggcccgggct ttgtcttatg
      721 tgtgtgcata agtagcaggg agggaaagag ggaaaaggct ccaaaagcac aatgtaattg
      781 aactttccac attatttctc actaatatgt gctgccactt ggtctcatta gtggaatgat
      841 gtgctctgcc attctggaat gaactctttg cccgagtaat taaggctcct aaagtttcta
      901 gtcctttgtt taaagaattc gtccttgtgc tctccatgcc ccatcagctg cctaattagc
      961 aggacctttg tgtgattaaa aattctcaca gagattccgt tggatatttt cctctgttaa
     1021 tgagtgtttg tggacagctg ggaacgggcg caggctccct cctcgtaaga cctcctgtgc
     1081 attgggcaga cctctgtcag ggcccgagag tgagagctgt catttccgta atggggatgg
     1141 aaatttgtca aaacccaccg aggttcccgc aggaatggaa atgccttgaa atataaatga
     1201 ggatgaatat tcatatttct ttcactttga taaaaaaaaa agtgctgagt gttaatatac
     1261 atgtctgtca gttaaagccg caccaggaac tttgtgtgct atgtatgccc attaacgcag
     1321 cccttggctt catttgctat tatcctcagg ttctttttct acttaaagtt ggggtcaccc
     1381 tctccactga gaattgcaca ttggaggctt ttccttttgt tacaattctc ccacttagtg
     1441 agtgtctttg agaaagagag agagagagag agagagaagg ccctgaaaga gccagaaaga
     1501 catttaatga agcccactcg gaagtaaggt aagcaggggt caaatccaaa tgaaacgaac
     1561 aagcctgcta cgtgtgcagt tggaggacag cagaaatccg gag
//
LOCUS       KCVB01000457            1771 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21442pri ultra-conserved element locus
            uce-1270, sequence.
ACCESSION   KCVB01000457
VERSION     KCVB01000457.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21442pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1771
                     /note="ultra-conserved element locus uce-1270"
BASE COUNT          510 a          379 c          379 g          503 t
ORIGIN      
        1 gtaaaaaaaa caccagaggg taacggcctg actctcatct cacttcctct tttaagaaca
       61 aacccgagct tagaggagcc cccttcacac aggacgttca ctatctccat cacacagcct
      121 ggataatgat tctctgttga gctggactgg gtcatcaaaa gacatgatcc aacaattatg
      181 aggctatgta gtaagctgac accccataaa agcacctgat ctgcggacga ggctccattc
      241 atctcagcgg tcttcccatt aggcacgagg agatgtgcag agagactttt actcatttgg
      301 agatgtgact cactttccaa ttcattctta ttcaagtcaa aaataatgaa gcacggtaca
      361 tacagaacat ctgaaaaata tgacctgaat agagtagtga aaggggaaaa aatatgagtc
      421 aaacaagcct cagaatcctt gatcagcatt aaatggctct ttggttttat agctccaaca
      481 ggggtgcccc ccttcacccc ctcaccccta caccgcccac ccctgcctct ggatgaagat
      541 gcctccgctg tgtatgcagc agcctagagg cgctttaatc agactacgta taaacagctg
      601 ctacgcaggc tggaaagcag ctaaacatac ttgactggtc aaaaagccac taaattacat
      661 tgacagaatg acacgtgtga ttaaaagaat ccatgccgct tttacctttt tgcgcttcac
      721 acctacagtg gcacgacgac gagagccagc aactcccacc ccgctaatta ccattatcag
      781 tgaaatggta gggtttgact cattagttcc atgtgcattt aattagaggc aggctgaaat
      841 tggatttaac ttattacttt ttcatggatc actttcttgt catcacttca aattggattg
      901 cagccccagg taactaatta tgccagccgc aggatcagga gtggagaaat aggtaattag
      961 gaacaacact gtcggaccct cgctgatagt ccaccagcac caaggtggga ggagggggaa
     1021 attgaagctc gtttgtggcg ttgctcacaa gcctggctgg ttaactacac ctcattgcaa
     1081 gagatcctag gtaggcatcc acctcctaca ggggattatg tggcattcag ctgggctgaa
     1141 gatgttatgt caacatggtg tctttactat cagtgtaaag cccagattat gtgtgtttcc
     1201 catccaggat tgtgcctgag attggccttc attattcatg tcactggtac agtgtgagga
     1261 atggccagca gccagctgaa gttccctgtg tggacgctgc ttgaatgaca ttgaatgttt
     1321 tttgtcagtc gtatgtctgc actggttgta atgagaatac ataaatggcc agtttgagga
     1381 gaggcaggtg cacacatgta gatttagttt atggcaggtt taacttgtga ttcagagcag
     1441 agatagtctg cttatagaga agggtaaagg aaaatgataa tgtcacttca tttactattg
     1501 aaagttttca tgtttcatgg tttaagagct tttgcaaaaa attgaagcta aaaaaacatc
     1561 tctatctttt ggttttcata gtttttttaa tatttgatgt aattatgcga tgacctgttt
     1621 gttttgaaat aagaaccact actcagtatt ctctgataaa tgttgcattg aacattttca
     1681 tgatgtcaaa ggtgcatcaa ttgttattct tcagacacag agaaactact gatgtttcaa
     1741 agccatgcca gcaaacattt ttttatctct t
//
LOCUS       KCVB01000458            1197 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21485pri ultra-conserved element locus
            uce-592, sequence.
ACCESSION   KCVB01000458
VERSION     KCVB01000458.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21485pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1197
                     /note="ultra-conserved element locus uce-592"
BASE COUNT          308 a          220 c          325 g          344 t
ORIGIN      
        1 aggaagcaag ctaatcctgt gttacaggga tgtataatga tatcccaggg cgctatatga
       61 tgtttctctc ggtatggtgc cagcttcctg ggcctctggc gctactcttt atgggctctg
      121 gcccagcccc agcaccccaa tgccagccat agcctttagg cgaggggcag gtttttttta
      181 aggccttgcc ttgtcacttc ccaccaggga aaatacaaag caatgacaca aagagcaatt
      241 tcttggccat tgcttgtcat caagctaatt gagaaaagag ggtatgcttg gagtctgggg
      301 atggcagaca agtacatgtg gcagaggggg gacttcttga ttggcagtgg ccatgtggga
      361 gaaacgaata ctttctgtgt gtatgtgtgt gtgtgtgtgt gtttttgcgt gtgtgtggac
      421 gtctatgtgt gtatgcgtct gggtctatgt gcgggataat cctagagaat ctctctcttt
      481 gaaggggaac agatgtagat ttacatatgt ggaggctccc cagcctgtga tgagattagc
      541 tggttgggat ctttcttcac agcaactgga aggtgcatat ttaataaatg cctggagcta
      601 ggctggggcg tgcggggctt ggccctcaga gagccacagg acaaaggcaa acgaggccca
      661 tggcaggctg gcccatatgg gaacagggtt gcttacagat tgcactggcc tttcattggt
      721 tctgagacta agctttaagg gtttatatac agagagagtg gagcacttgg tgggccactt
      781 ctgcatcctc tttacaacga gaggtaataa agggattttg agcttgtcgt ggctcatctg
      841 tgtgcttgtt tgcttgctag ggtgcttgtg tgtgtgtgag agagagagct ttgcatgtgt
      901 gagaaggtaa atatgtgtgt gcatgtaagt aacatagctt aaaacattaa atttaataca
      961 aaaacataat aacataaaca acaaaaaaaa ggtgatgtaa tataaaccta aactaatgca
     1021 acataaacct aacataacgg tggctgtgag gagaatgtca aacatgattt gcacttgtca
     1081 gctcataact atttatttca agcaataaag cttctgagtt gttgggtttc tgttgttcgg
     1141 tgcattatcc ctctcttgcg tcaggtaagg ttgtcaagtc ttgtttacac aaagaac
//
LOCUS       KCVB01000459             931 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21561pri ultra-conserved element locus
            uce-945, sequence.
ACCESSION   KCVB01000459
VERSION     KCVB01000459.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 931)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21561pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>931
                     /note="ultra-conserved element locus uce-945"
BASE COUNT          269 a          180 c          214 g          268 t
ORIGIN      
        1 cctccaaaga atttggccat atggtcgtca acacgtatac ccagagagag agagcaggcc
       61 agggaaagac tgtgtgagat ggtaagggag aaagtgaaag agaagtgtct tcttttgtca
      121 gctgaggcag caggaaagac agtttaaaac agatcctata accctacctt agatcagatg
      181 gctcagacaa aaaaaaaaga gaaaaatcct ccactgcatc ccctactcca catatttgtg
      241 gagtgggaaa atctgtctca attaacacat ttgcattggg cgatgatgga ctccaaatag
      301 gtcactgact caatctcccg cctccgaatc ttccctctaa aatgttaatt aaggagggct
      361 ttgagatttt tttgctcagg gctggccata tgggccgggc atatgttcta tatatatagt
      421 acaatatata attcatacac tgctgcagga gagagtgttt gggggcctgg agtttttggg
      481 gggtttacta caaagggaat cagtatgttc tggcctccct agtggcaggt acagagctta
      541 gcctattcat tagcttcatg ttattcactg ttgcacaatt ggattgaggt tcatcaacac
      601 ctgtttcttt ggctgacgac ataaagaggg tgtttaaatt cttctttgtt ggttggttaa
      661 tacaaaatga aagcaaatga tcttttattg gagtgcagtt gctttttttt tgtattacat
      721 tgtttgaatg agctgtttgg gtgaagttta aggatgagta acattgagaa tctttgaata
      781 tgaaggacta catgtgcacg gagatattct caaatagtta gtatgttacc tcacacactc
      841 ccagttgaac cagttgtccc agtgccatcc tcccagacac ccattcacta gcagaatgag
      901 gcagtggcag ccaatgaaaa cagctcactc c
//
LOCUS       KCVB01000460            1352 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21599pri ultra-conserved element locus
            uce-106, sequence.
ACCESSION   KCVB01000460
VERSION     KCVB01000460.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21599pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1352
                     /note="ultra-conserved element locus uce-106"
BASE COUNT          335 a          357 c          269 g          391 t
ORIGIN      
        1 catcacaact ttctcatact acaatgcttt taggaaactc ttttactcaa tatgaaaacc
       61 acagactgtg cttttgtcag ccctttctca tgtaaagaga caatatttta accagtcctg
      121 tcacagtcca taaccagaaa tgctggaaag acagacggcc aatattcctc ggagtgtagc
      181 tcatcatatc cacttgctct gattcaaggc tcttcctctc aaatcaaagc tcacgtgctg
      241 ggcctgctct gtttggggag gtgtgagagg agcgcgagag gagttgggag tgctgttgcg
      301 tcgtggcttc actctgttgt aacccacaac ggagccacct cacaccccca acgggcttcc
      361 acttcaagag ccctgccttt gttcaaaaat gtatttgttc tcttattctt tttgggaact
      421 gtgtaaaaca aaaaaggacg aatggcgaaa agaactattc tttttccctc cccaccctcc
      481 gacaccatcc cactaccacc tctctccctc tctctcccgc tctgtctcta actctctctc
      541 cctctctggt agtgcatatt ttgctgcaca tgctaatgat ctcccccctc tgtattctga
      601 tgcaaggtct gtctacactt ttccctttca tccacataca aatgtgctct tattgcctct
      661 tcgcccccgg gcctttaaag atgccttttt gaaatatgcc gtttgataag cacatcattg
      721 gccaagtccc cgcagtgatt agagctctct ttaatgtgag caagttagag agtgaatgag
      781 ggactccggg gccagcagct atccgttctc ctctgacctc accccttgac atctcccggg
      841 ccactgggga gggaccgatg tcggggagga tgaacccaag tcaagctcag cacataggca
      901 ccgttggcca tcctccatcc ttcatctcca catagagatt aagcacggca gaagagacac
      961 tggagaatcg ccttcataga gacaaggtac tgtatgctga aaggctagat tcctgtactt
     1021 tatcacaggc atatgacagg aggactgctc tctaatgtct tttaaatata ctccattgat
     1081 ctcatgttca gagtcttgct ggcaaaattt tttttttact tgccaatagg gaacactgat
     1141 acagtcgtca ggtgctttct cctgttctgc ttcttgcatt caacacctgt tgagcctttt
     1201 gggataagta ggttatattt aaagtgcttg atttccagcc tcctcacagt tacatttcag
     1261 gtagattgca acatttatac agacacagcg ttcaggaaac gtgctcggca tgccacagtt
     1321 tgaaacaaat atcagtcaga aagtgtaagt at
//
LOCUS       KCVB01000461            1752 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21664pri ultra-conserved element locus
            uce-1171, sequence.
ACCESSION   KCVB01000461
VERSION     KCVB01000461.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21664pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1752
                     /note="ultra-conserved element locus uce-1171"
BASE COUNT          428 a          329 c          501 g          494 t
ORIGIN      
        1 cattcagcgg ccgaggctgc ctgccagtgc tatgctcggg gcagaggcct gagttgactg
       61 gacgggggtg gagaacatgg gggggggggg gctggtttga ggctctgtga ggggctttga
      121 gtgggcctga gtgtaggcgg cgttttatat gaaagtcggt gagggcaggg cgcttgaata
      181 ggggtggctg ggaagttggg ctgggaaggt ccaggatgca ccccacagtg cttttgtgtg
      241 tcctcacatc cgtgtgtgtg tgtgtatgtg tacggggagg tggtggtggt ggtagggggt
      301 agcatatgtg agatcatgtg ctcatattca ccagtggaat gtaaaaagaa aggggggggg
      361 gcttctcctc tctcctctct ttccctctgc ctcgttgttt tctgttctct ctctgctgtg
      421 ctgtagtgta ggtctgtttg tataagctct ttacaggatt gagcacgctg tggagccatc
      481 cagatgaaac aaaaccaacc tcatttggtt gttttgaaaa tgattctctt tattttgctt
      541 ggttcaggca gatccacctc gtttgacatg aggggaataa aggctctctc gcgttcaggg
      601 tcatttccca gaaacatcgt ttgtatctgc acggctcttc ctctgtgcac tcaaactcct
      661 ttcatcaaca ggtcttataa agtctctaga caacatgaat gaaagatgca attcaacctc
      721 cctttttccc ccctttttcc ctttcgacct cctcaaaagt acaataatct tcagtcactc
      781 gtctcctcct ctccattgcg tggctgtgtg tagcaccgga gtggtgctga ttaagccaac
      841 agagagacaa acgggagaca aagtctcagc ccaccagttt agtttcacat cctggactgg
      901 agctgggccg cagcttgcca agtggagggc ttttaaactg ggctaaacac taattgctgc
      961 cctatttctt ttatgttgct tctttcccat ctgcgatggc gcttccagac ctccacagca
     1021 gccagagcct cattatagca gacagttaac aaaggctggg aaacagaagc ggagaattca
     1081 cttaggatag ctatctaacc gccaactagg aggcttgctc agaaaattga atcaatgaca
     1141 ggggccggct tgtgattaaa tccttcaggg atattaagat attaagattt tggaaatctt
     1201 aattattcat ctaggttgtt gcggctcgct ttgataatgg aataaatggg ttgtgaaact
     1261 aagaagtggg gtgtaaggct cattgtttta tttgagagcc aggcttttca gaatttttaa
     1321 cagaatattc gaaaataaaa gtgaccaacg atttccctac aaagaaactc gaaaatagta
     1381 aacacctact tgttgagtga aacaaatcga ggtctaattg tgatgttttt tttcccatct
     1441 cgcttgtttt tctgtaggtg ttttttgtgt gtgtggagag agagggggag tgaggggagg
     1501 atggatggga gggggttaga gaaaacagaa tgagggaaat gttgaggagg tgaatgagag
     1561 ggagggataa agctagaggg aatgagattg gggagtagaa atagaggggg ctgagattga
     1621 gggaggaatg gatagaaggt ggtgaggaga gggggtggtc agcaacaaag cctaatgttg
     1681 ccatggaaac ttgtttatgc ctcactgatg catcttgtag atccaattag agactgattg
     1741 tgtccgtgtc tt
//
LOCUS       KCVB01000462            1426 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21741pri ultra-conserved element locus
            uce-282, sequence.
ACCESSION   KCVB01000462
VERSION     KCVB01000462.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21741pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1426
                     /note="ultra-conserved element locus uce-282"
BASE COUNT          379 a          340 c          299 g          408 t
ORIGIN      
        1 atgcataatg tgtgtataag ggtgtaacaa cactgtgtaa gagcaagtgt gcagatgtag
       61 ctgcttgttt tgacattgtt gctgccttgc attttatatt tactcaatgt aatcaaagga
      121 tttacagtaa aacacagttt tattgtgtac tctacttaga ctttaacata gtttactaca
      181 caatgaggcc accctaaagt gagtgttagc aagcaatccc taaagcaaga gctttgagag
      241 ctatgatgct tacacactat catccctggt taaaacaagg ggatttatca gggatgtctc
      301 tgcctcggct ggggctacag ggaccaaatt acacccccag tgtctggttg gaggcggaga
      361 aggaaataga tgggtccaga tataatagtg atggcccctg ggtggtactc ctgtgaggaa
      421 gaaagaggag attagagtgg aggtgtgagc tcgcagtaag attacatttt ccctcttttt
      481 actggataag tgcatctctt ttttttcctg tttcactccc actattctgc ttctgtttca
      541 cagtgttcca tctctctctc tgccctccgt caccacacac acgcataaca cacacacaca
      601 taaaaaagtg tacacacgca catatgcacg cacacacacc gaacacgcac atctctcctc
      661 tctccctctg tcaccctctc cctccatcct ccctcacttg atccttacac ctacgtgcta
      721 cagatggttt cattttataa gagataaaac cacatttggt ggaactgatt aaacagaggg
      781 aacaacatcc ccatctgcca tttattccaa tctgaaattg cttgggcaga tgctgacatc
      841 tttggagatg attgattcac cccgtctggc cagagaggag tggagaggat ctggatcttc
      901 cccggggctg tggttcctgc ctccatgcca ttgcactacc acagtgtcac ttcaccagtg
      961 atggagcccc cagccctccc cactcctcca tctcttgtct ccagcaagct gctgcaatca
     1021 gcacaggccc tgattggact tgggcaggtg agcgcaatgc actttaatca tggggccagt
     1081 acatgccaga tcatccccaa tgtgttggtg tgtgactgtg tggttgcaga gagagatggg
     1141 gtggcctgtg tggttcaccc atattatgaa taaacatact ttctaataaa actttttggg
     1201 ttttattagc tttgatttaa gataagccaa taacaactag ttcaacgtag aatacaatgg
     1261 aggcaatgga attttgtttg tatgtgttaa cacacaacat tgaaaaatta catttataca
     1321 aaactaagag tctaaaactg tctagtctcc catagccagc cccatctcat ccgcgctgtg
     1381 ttagcactgg agaaaggtct gattgcagct gttatcattc tgcttt
//
LOCUS       KCVB01000463            1228 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21799pri ultra-conserved element locus
            uce-1208, sequence.
ACCESSION   KCVB01000463
VERSION     KCVB01000463.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21799pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1228
                     /note="ultra-conserved element locus uce-1208"
BASE COUNT          357 a          269 c          279 g          323 t
ORIGIN      
        1 actgaaaatg tcctgcatat aatcagctga gagtattttt tctcacagga cttgaactcg
       61 caaacatgct gaagaggttt tattttacag tcttttccct cttgcagtag gattgccagg
      121 cgctgtcaaa agcccagaaa gctcggcctg gagtcgacac agccagttag tcccgagccc
      181 ataagcatca cgctgggccc gacgtcgaac aagtgcttgg tgaccttaca gcaccagctg
      241 gagtttaaag aagtctttaa aaggctgatt ctccacagta atatcatcgc ccatgcctca
      301 aagagtctgg attaaggccc gctttccctc gctaaaatct aacaaatgtg tgaaaaaaca
      361 tcacagccaa agccaatgcg gaaagaacaa tggagggagg aagaggagag agagagagag
      421 agagagagag gaagggagag ggagatagga aaagatcgga gaggcagaaa agaagggaga
      481 aggtgagaga gggaaactca acatcttaac accccccccg atgccccctc cctgccagat
      541 atatcaatgt agttggtttc tctgaaattc aaatggcaag agggaacaaa aacagatgaa
      601 aaaaaagaaa cctctggctt tgcaaagggg aggggggggt gctgagaaaa agacctgcca
      661 tctatgtatt agtgctctgt aaatattaca tgagctccac attcgctcag ttgtttggca
      721 ttacttccac tggatgggta tattatttta gaaaccatgc cttgtttatg gagaggcatg
      781 acctacttcc agacgctcct ctctctcttt tcctctctct ctccctggta tttttttctt
      841 tctctccccc atctatcttt ctcactcttt ccctctttct ctctctctct ttctctttct
      901 ctttctcact ctctcttatc cactctcaat ctgcgaaatc tcaactggtc gtccctgctg
      961 aacaattaca aggcagagag acccaaatgg agatgtttgt agaaaacaac atgtgtataa
     1021 ttaaggttta atgagctctt gtcagggtgg aatgtgcaat tagattggta ataaaaaatt
     1081 ctgcctgaac gctagtggca gaggctggat gtggagtcag catggcagcg aatgaaagaa
     1141 cagtgaatta ttctgagccc tattgacaga agtagattgt gctggatgct gcatttagat
     1201 ttaaatacat ggtgagctcc taaataca
//
LOCUS       KCVB01000464            1619 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21833pri ultra-conserved element locus
            uce-552, sequence.
ACCESSION   KCVB01000464
VERSION     KCVB01000464.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21833pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1619
                     /note="ultra-conserved element locus uce-552"
BASE COUNT          489 a          308 c          363 g          459 t
ORIGIN      
        1 aggtaacacg cttcttattc caattcttat atggactaag gctgccacct ggtggatcat
       61 aaagacatca gcaaccaagg tccacccatt aaaataaagc acttaatgtg aactaaatct
      121 cgtttttcca atataatggc agtgctttaa acactggaag atcttcaaca taggggcaat
      181 agaagagttg agtcgaatca tgtttgtcct tgtgtaagat tatttgatca tacagttttt
      241 actctcctag gtttcacata atgaatttgt ttctggggaa taacttgtac actaaaaact
      301 ttaaatggtc caagagttta atctcacagt ttcaggtttt aaaagtatat ctttaatctt
      361 cacagtgtga agttgactgg cttgaaaaaa gttagtggta ccatggtgaa cccaacagtt
      421 ttgtctgtgc tacaaataca gggtaaaaat tactctggtg ctgaggaaac cctgattgat
      481 taatgacagc atgatgcagc aaggagacac ttgagttatt aggacaaagg gtagtaggac
      541 atcttacagc aaagatcatt ttaatggtcc caaatattct gaacaactaa gtgtacactc
      601 atgtaaaagg aaaaaaatga gaagatatga caaaattcat cacatctaga attccactca
      661 atctttaatc aagacgggat tacaaattct ccaaaaaatc tgcagtttca aagggcaaaa
      721 agggtaaaac tagctcacct actgaggtct ttaaaaacct gttcaagtgt gactgaaaac
      781 gtcctgaaca tttaaaggca ggtctttttt gttgtcattt acaaaaaaca ggagaaaggt
      841 tgagacaaat agagaacaaa aagaaaataa tgaagaaact gctggctttt ttttctcttc
      901 agagaaagaa cgtccactga ggaacgacga cactgatgac atcatcaaac gacaacaagg
      961 acagctcttt gccacatgct gcctcccgtc tggtcctata tactaactta aagaaaaaaa
     1021 gacaaaaagc aacagacaaa gacaggaaga aagcagtgga gaaaagaagt agaaagaaac
     1081 aaggaggaag gtgaattctg aggagggggg gattggggtg cgagtggtgg ttctgggtct
     1141 gaattggcca acccatttgg gccgctgtga tgtccttccc tgagtggggg ggcggggggc
     1201 agtgggttat ggtccgagcc cacgagctct ctgggatttc actctgtgtg tacgtgtgtt
     1261 tttgtatgtg gtcttttcct ccagttgtta aatcaccaaa ctcaaccagt ctcatactgt
     1321 ccgagtctga cgatctgttg agtgttagct ttttccccaa ctttcacgcg tctgttccgc
     1381 ttttcagacg gtttttcact cctggtttcc tgattggcac ttgtatgaag tgggctagtc
     1441 tgaggaggct tcatatccgt tcttagtccg ctcctctttt gaccacccgt tgacggtgag
     1501 gggtggcttt cattcattca atttgtttac aagtttgatc attcctgtgt gaagtctatg
     1561 ctctgaaact gtctgagcgg agcacgagct gagacagtga cgcttcttta ggagctcag
//
LOCUS       KCVB01000465             998 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21874pri ultra-conserved element locus
            uce-183, sequence.
ACCESSION   KCVB01000465
VERSION     KCVB01000465.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 998)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21874pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>998
                     /note="ultra-conserved element locus uce-183"
BASE COUNT          272 a          263 c          188 g          275 t
ORIGIN      
        1 tttgctacct cctctctttc tcagtctacc ccagccacgg tactccccca gcccacccca
       61 taacctcgta ctccctctta gagctgcaag gcacagggct gtgctttatt tctttgctca
      121 tttttctcag agattggtac cctcacacgc agatccatac catgctcctg tgcagcgcgg
      181 gcggtggtga agaaagaggg ttatattttc cccttgagtt aggacacctc atgacaaatg
      241 ataatggata atcaataaag cgggaaatat gaggcagcaa actttatgaa gccaggagac
      301 atattactcc ccaacaacac tggtttccct taacaaagtc actactcgac tgtgcctaat
      361 aatctgaagg aaaatcaaag gacccgcaac cacatcagca atattggcaa cttattccac
      421 tttaatggca ttttgattga ttttttccct cttcctaatg gtcgttttat agatttgacg
      481 ctgcttctgc aaaacagtag tgtgtaataa acctccccga aggcaaacgg cagccattaa
      541 agctcttgtc ttactagcac tcataattgg aggttgacca ccaccacatt ttggtctctc
      601 acataacaca caaacacaca cttccaggat taggctcctc tctctctctc cttgtctctc
      661 cagacctttg atatttttgg tagatggcca taataagtga taggatccct tcctcctgct
      721 ctttttgagc gatggggtta aaaatagcca taaagaggtg accttggcgc tgacattgtg
      781 gaatttcagc caagcattga cctgatgaaa atggaaggcc aggccgctaa taaatcagga
      841 tgcttttaaa atgagtttac cctaatgctc attcatcacg ggctgtaatg ccatttgcag
      901 gcccaggatt cgctgctgtg cacaaacaca gcagtaaata acaacagagc ccttgcatcc
      961 ctcgcatcgt tgcagcctcc aacatcagca tacattta
//
LOCUS       KCVB01000466            1604 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21913pri ultra-conserved element locus
            uce-985, sequence.
ACCESSION   KCVB01000466
VERSION     KCVB01000466.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21913pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1604
                     /note="ultra-conserved element locus uce-985"
BASE COUNT          423 a          323 c          313 g          545 t
ORIGIN      
        1 aattttaatt gcttatccag aaatagttcc atgaatataa ttggtaataa tgtcagaaat
       61 gtagccaaac acatgcacac acatatgcac atacactcac atagtctaca aaaagtcaat
      121 aagacaatta atatgccctt tgagatacac aagtgggtag ggcaaagaac tgctgacaca
      181 caaagtccta tacaatgctc ttctaattta attataatgt aagaagtcat tgggcacatt
      241 gtagaacttt gtttgttgtt tactatgata aaatctgtta tgaaagtagt ctcaacatac
      301 catctgcatc cacttcacag ttttcgttgt ttaatgacac aagtatattg tattaaaaag
      361 aatggggaat agactgctga tatcaatgca agacttgtgt tactgtagaa tcacatttgt
      421 cgttctttta ccttaaaaat gatctacttg ttctatatga tcatttttgt gtgctggcaa
      481 tatacaaatt gtggttttgg attacaaata atggagagtg gtcacttagc tataaagcct
      541 gcgagcttgg ctgtacaaag tgttatatat gtgtgagtgt ttgtggctgt gctgttttca
      601 atatgcctct atttatgtga aggctatcca gtgtccttgg acctccacag aggtcagcta
      661 tcacagggct ctttcttcct cttttttctt cccccccctc ctctcactct ccatctctcc
      721 ctctctgtct ctggctgtct cattcaaagg cccgtgctca ccctacaacc attgcaaata
      781 actgccgtcg cctttgtagg tcgggcgaat tacacaacgt gtcatgttgg tcgtgtagca
      841 ggtagcaggg cccatgggag atttagtggc tttaattaca tttctcttgc tggccgttga
      901 ggagaaagga attaaatggg actgttttct taatcatctc gcgctcagtg ggtaaccacc
      961 ctagctttcc aaatgaaaaa gtgcattgaa gcataaactc tatgctgcat ccagtcatgc
     1021 ttatgtggta aacaatggtg tgtgtgtgca ctcgtacgca gtgtgttttg agttttgagg
     1081 atggacattt attgccattt ccatggtctg ctcagtgtgg gttgtacttt atctgtagag
     1141 tcggatggaa atacgtttct attgattgct gtccttggaa agtgaacaga tgctggcggt
     1201 ggtgttgaaa tagtctcagc attaggaaca tgaacctttt gtgtgagcag atagatctca
     1261 cactgcaggg ctaggcagga ctaagaatgg ctttcgaaaa gggaatgcat gattcattgg
     1321 atgtacaaag cttattacag aaatacaata gataattgta acagtgagga aaaccaagcc
     1381 aataaaaaaa aaaagctgtt cattgtcgtt gttttccttt attttttccc tcagctcttt
     1441 actctgtttc cctctcgtat gcctccactg atcacttgct caccttctgc gtctcatgcc
     1501 ttcctacaaa agtcttcact ctttatacat tgcttttttc catttgattc tctttttttc
     1561 ttttttttgc ttccaatttc ccatttccat tgtgctcttt ctgt
//
LOCUS       KCVB01000467            1893 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 21997pri ultra-conserved element locus
            uce-362, sequence.
ACCESSION   KCVB01000467
VERSION     KCVB01000467.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="21997pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1893
                     /note="ultra-conserved element locus uce-362"
BASE COUNT          540 a          408 c          400 g          545 t
ORIGIN      
        1 gttatttcat gttttcaatt ctgttgtttg ctctggtagc cacgtatatt tacgaatgga
       61 ctgtatgatt gtaagtggag gctactgtgt tcttgcaatg tgttagccac agtgcatttc
      121 ctgtgcagcc tcctcttcca agcccatggg aattttatgg gccattggtt tgtagcgtgg
      181 cctctgatat catgatttac aacctgatct ttatcaaaaa atctttcccc cactcatcct
      241 cttgcaccct cacgtttttt atggttgcat gttgtatcac ttgtcatgga gaaaaatcac
      301 gtcccttcca gtgctgctct tgtggcagat gtggtcaaga tgcaatcatt ctgaaatatc
      361 aaatgagtca ctgcatgtca tacaaccaaa gcagatgttt atggtttaag aagcactgtg
      421 tgacatttcc tcaattaaag aaatcatgtt ctaccttgtt actgtatgct acttagagca
      481 caaaattgtt gtgtgtgctg gtgcacgaac atgcagctgc aaatgtgcgc gtgcattaga
      541 taaagacagg gagaaagatg gagagagcaa aagaaagaga cagcgagaga gttcagcgca
      601 gatatctgag tgcgtctgac agagaggaca gccccgaagg caggtcccag cccttcagca
      661 ccgaaaatga cacatgactg ctttgacttt tgtcatccct tgtctccctc cgcccccaaa
      721 cccaagcctg caacaaacac tgaggctagc atagatacac acactcacac acactcaaag
      781 acacacagtc acacactgta tactgcttag attacattat ctttatcccc aggacatgag
      841 ccctccttgt tggatgacat ttgggcttgt gcagcactaa ggttaccttt ctttgcccct
      901 gagttaaaga tgcctgtctg cagcatgcat ttgaaatgaa gggcaggaaa aaaatcagat
      961 gagaaagatt tccaaaagca ctttgatcag aggcatcaac agtcacgcac aaacttgtta
     1021 agcagtatgt aaatttgggc tgatactgaa taaagttgcc agaatggcat gaatccattg
     1081 tgctgcgatg atcaaaataa aagagggctt catacgctgg agggcaagaa agtcacagac
     1141 atgaaggttt tttttccttc ctcttttttt ctacaaaggg gctgatggga tgcagagagg
     1201 agagaaggag gggagggaaa tgaaagatag gggaaagcct ggaaggatct caatctaaat
     1261 tagccgagta gaaggaagac tgtgctgagt gagacatata attaaaacca aagcgatgaa
     1321 aacatcagta ctcattcagt cactcgcccg atgaatttct gaaattgcta ccatgatgat
     1381 ctgaaaatgt ttcaaagcta aaggtaaaac caatctattg ttaaagcaca ccagtttccc
     1441 atttactcta tagtggactt cctcccacga tttctcttca tccacctcct ctctatttct
     1501 ttatccctcc ctcattccct ctttttctct ctctgacacc ctgagatgaa tagatgctgt
     1561 cactgagatt cttgtaggct gtcagctttt aaacaaacag ttagcaaaaa gatcgctttc
     1621 acaggaaacc tcacagtctg aggttaggtc atcagtgtta gcttgttgtg tcctgttagt
     1681 actggaacag gactttgtaa taaaagtaag gacagacatg tcctggtctc aggcctcgta
     1741 tattcaaact aagtgctgtt ttgaatcatt taattcataa ggtagcagtg atagcgcttt
     1801 tctgcatttt gccctcaatg ctgaaatttt tacctattct tgtagcctgc atatgcaacc
     1861 aaatgtctct ggcagttaga gaggaagcgt ttt
//
LOCUS       KCVB01000468            1070 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22040pri ultra-conserved element locus
            uce-898, sequence.
ACCESSION   KCVB01000468
VERSION     KCVB01000468.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22040pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1070
                     /note="ultra-conserved element locus uce-898"
BASE COUNT          280 a          240 c          230 g          320 t
ORIGIN      
        1 atttaatgag acattactct tcatctgaga atagctactt ataaatacac cctgataccc
       61 aagatagagc atacggtaaa gaagaacaac atcactaaca tcagggtagt gttttttgtt
      121 cttgaaagcc agatgggtga acaagagaac tcaccatcag aggacagata tgttaatttg
      181 atagggagta ctgcatccct ctaatggcac aagattagag gctgatcaga ttaggatggc
      241 tctcttgtgc catgcttttt atttttagaa acttgagaca ccatggggac acataaagac
      301 ttttctatta ctgcagatgt agtcagatca acatctcaaa gcaaatgcat attagaaagg
      361 acagtattca ttgtaagagc tgcttttgag gcctggaaac atgtttattt ttagaggcat
      421 tattggctga tttgctttgt aatcacagca tgtttttatt ttattgcctt ctgcccagaa
      481 tagaaataaa atgatctctc ctcctcctcc tccctccctg tgtttctcga ctttgtagaa
      541 ctgtagctct catgtaagga gggccgtggt cacctgcttc tcggctggct gctgtgggcg
      601 ccatggcaac cggcctgtcc gctactgcaa gcgttgccat gtcaaccacc acagcagtga
      661 ggtgggggct gcggcggaga cccatctgta ccagacctcg ccccctccca tcaacacgcg
      721 ggagtgcgga gcggaggagc tagtgtgcac tgtggaagct gttataaggt acattcacag
      781 tgtttacact aacgtatcac agccacagtt acagggcata tgcctgtaat gctgtaaaaa
      841 catgttttac atgagtatat agacgacaat actgcattaa attagattat tattgcatat
      901 gcagtgttag gtctgtgttt ttatttccct tctgctcttt ctttgctaat atgtgtctct
      961 catgtcttct ctccgcctct gtgaatccct ctgttatccc tttcatcctg tctacatcca
     1021 tccagccttc tcaaggaggc ggaaatacat gcagaacagc gtgagtttga
//
LOCUS       KCVB01000469            1195 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22085pri ultra-conserved element locus
            uce-865, sequence.
ACCESSION   KCVB01000469
VERSION     KCVB01000469.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22085pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1195
                     /note="ultra-conserved element locus uce-865"
BASE COUNT          306 a          294 c          262 g          333 t
ORIGIN      
        1 tttattgtcc tacattattt taacaaatac cccactgatt ttctagcttc agttacattc
       61 actgatgtag taaaaaatgt tatggttgaa cttagggtga actatgactt ctattagacc
      121 ctgtgcagcc agcaatacag ttaacaatag tctatgcaat ataaaaatag agcggagaag
      181 attttataca aatacttgct actgtatgta gcagtgcccc cctctctctg ctttagagct
      241 agctcaatgt tatgtaatat tttcaagtac tcttgtctta tttggtgtac acagtctata
      301 agggattacc tcggctcact ctcccctcat aatctgctga tgatgacgtt atactgcagc
      361 tcctgagctc cagatctcgc gaggttatgg aaaacaatcc agacgccctg tctctctcgg
      421 ttcccccctc ctccccagcc ctcccttccc acgccccagt gcgctgtggg atagtggcac
      481 tataaagtat atcctctctc ggagaaagat aagaagcccg agacaggagg gggatgaaga
      541 tggcggacgc caagcagaag aggaatgagc agttaaaacg atggctgggc tcggaaacgg
      601 acttggagcc tcctattctg aaaaagaaga agacgaaggt gaagttcgat gatggcgccg
      661 tgtttctggc cgcctgctca agcggcgata ccgaggaggt gctccgaatg cttgaccggg
      721 gcgctgacat caactacgcc aatgtagacg gtctcaccgc cctccaccag gtttgtggtt
      781 gtctgctatt ggtcttaacg ccaccaactg gcattgccaa catcattagg cactaatgtg
      841 ggtttgctac taccaactgc ttgccctgga acacctgtca aatggaagcc attctcatcc
      901 cgttgtttac ctacctagca cacccgtcct tttcgacgca gagtatcgtt agagatggac
      961 actctggctt ctctctgacc aaaccgaaaa cagtgctaac tgtgcatgca gttttcagag
     1021 tgcggtccat tttaagcaac aaagttaaaa tcttcgcttt caggacgact ttttgccccg
     1081 gttaactagc tttagtctat attcccagcc agtgggtgca gtctaattac tgtactgctg
     1141 catttcaaat tgtaacgttg ggtcatttta tgttgtaaat tacattcaag tcgag
//
LOCUS       KCVB01000470             795 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22118pri ultra-conserved element locus
            uce-22, sequence.
ACCESSION   KCVB01000470
VERSION     KCVB01000470.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 795)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22118pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>795
                     /note="ultra-conserved element locus uce-22"
BASE COUNT          267 a          121 c          189 g          218 t
ORIGIN      
        1 acacaattat tgatactgat cttaaagaaa atccattaat ggtcaggctt tactgtatat
       61 acttacaaaa tgttagtgca ctttactgat tagaaagaaa aggacaaact tgacttgcca
      121 caacagttta caacactgaa aggtcattgt tagttttcac attctgtatc aacaaaactg
      181 tatccataaa gattattgaa tgatacagta tcaggcattg tatcaacaaa gactttttgt
      241 tgggttttaa gataaacttt ggttttgcgt ggaggggggg gaactaatgc gtcaactgct
      301 gacacagcaa gataagtgga taaataacac ttgtggagtg acacagacgg taccattgat
      361 gagacacatt aacaacagag attactgatc tatgatcaat taaagtgttc actatgatgt
      421 tgcttcaaag aaaaaaaaaa ttaaacatgg gagcagacag atggacaaag tgaagctgta
      481 aaataggcaa tgcaaaaggt aaataaaaga ttaaagaaac agacagaagg aagaaagact
      541 actttctgaa ggcattttac caaaatctct gtgaacacac agcagggtta actgtcagtg
      601 tgatgaagag agccctgtca gtcaggccaa tcacatctga ttggtgctca tgggtggcag
      661 actggtgaca ggcagccaag agacgcaggc gacgctcatc agcatgccag ctaaaagagc
      721 agtttgtgtg tgtgtgttta tgtgtgtgtg tgtgtgtgtg catgtgtgtg tatgtgtgtg
      781 tgtgtgtgtg tgtgt
//
LOCUS       KCVB01000471             958 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22149pri ultra-conserved element locus
            uce-67, sequence.
ACCESSION   KCVB01000471
VERSION     KCVB01000471.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22149pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>958
                     /note="ultra-conserved element locus uce-67"
BASE COUNT          251 a          230 c          202 g          275 t
ORIGIN      
        1 cacacacaca catacacaca cacaaacacc ctgtctctgt cttttttttt tttccagcat
       61 ggaggggcta tgttgctcgg cagaacctca agcaaataaa aaaggagcgg gaagaggccg
      121 ctatctgcat ccagtcaggt aaagaaattt ttaacatttt tcttctgaag tccttcatat
      181 attccagttc agacctctgg aatttctcat gtagtctcgt gtctggtgga aatacgcgtc
      241 acttttttca tactttttca aaaggaaaag cagataaagg cctgaaaacc cctacatctt
      301 tgtgaaacac ctttttgatc tcaatgagtg gcctgcgaaa cagtaataat taattaataa
      361 tgcataaaga aaagcttttg aaagtttggg cagcatttgg tccccatgtt tcagaagaac
      421 agtgcatact gagatccttt ttttcctttc atctctttcc cagtaaaggc aagagagggg
      481 cttatccctt ctgtttcctc acgacttcct ctctcaaatt cctggcctat tacacagctc
      541 tcacatacca gaaacaagat gcctttttat actgtcaacc ttttcatctg aaatgtaaat
      601 taattcttac tcagtgtgcc ttgacgaaga tcaaagtggg cacattaacc ttcccgggtc
      661 cacccccccc ccctctttgg agcctctcac tggaggacaa ggaagggtgc caaaaggggc
      721 aggagagagg agggcagggg gaggagattt ggcattgttt cctccagctc tcctaagggc
      781 tgccctcatc tggcacatgg agcatctcag tcgcctgcac accttgtgta aatgtcctcc
      841 attaaaaccc ttgtaaataa cgctgcagaa tgggtaaaca tggttttggt gtgagggaag
      901 cctctctgtc actgctgtct ttctctatag agggacgtgt ttgtgtgtgg ccgcgcgg
//
LOCUS       KCVB01000472            1358 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22214pri ultra-conserved element locus
            uce-1051, sequence.
ACCESSION   KCVB01000472
VERSION     KCVB01000472.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22214pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1358
                     /note="ultra-conserved element locus uce-1051"
BASE COUNT          373 a          289 c          315 g          381 t
ORIGIN      
        1 tttgtcatgg tggaactggt tttagtgctg agttcacgtt ctccacatgt gtggtctgtc
       61 ggcccagggg acctatccaa gaatcccaga cacacgtgtg caggcagaag cagtagtgca
      121 atgcagagta gataaacagt gctcttactg gccactgggc acactccaca tctaaacaac
      181 gccaagttaa ctactttgca ggattatgta gcaaaagcaa acacaaatac atacgtgttc
      241 atcccaagaa tgccattttg gtgaagactt ttttttaaca ctcaccctca cactgtgtga
      301 ggacaggagt cataaattgc aatctgcata aattgcacat gtgggcatta aatatgtttt
      361 aaaacatcac tccttcctcg ttgaatttag tgtcaagcat cttgattgcc ctaattcagc
      421 attccagcgc agcagtgcaa gtgtgcctca ctttcacagg ggagatgtag gagagactct
      481 agggatgtgt gcagcgttga cggacagggg gataaacaga gggattgtcc ctgacctccc
      541 aacaccagac tggaggaaga cgaatagccc cccaatgggc cctgagcaga taaaatacac
      601 cctaccgtct tcaacaccag gtgtgacagc catcaggggt ggcctatcag caggaggtac
      661 agggaagggg aggtaagggt gagggggcac attaggaaca tatcagcaat tactctctgg
      721 gtttattttt cttcctgctt taacgcctgg ataccaagtt acattcattc tgggtgttga
      781 ttgtatcttc ggggtctaag caagaacact ttgcagagtc atttgtcgat tatctgataa
      841 tacactcaag caagttaatg tgatgttaca gcaacgattt aaaagcccgg cagtgtgttt
      901 atttttagtt tttcttcagt gaattgtgtg gccttgcttc atcaagcttt atctgggaag
      961 ttcagttcca tcttttctct tagtgtaccc agagttgcaa atcaacttct ttatgtaatg
     1021 gaattcacag ggtcgaggac gtgtttacct ctttttccag gcccattagg caccaagcag
     1081 ctatatcata tctgacacga gagaatggcc tcaatctgtt taagccatga tctctgactg
     1141 catgggcccc aatgtcttca gactgacctc tgcaagcaga gatatgaaga gggatgatag
     1201 tcatttatat gtcctgaact tataacaata aatgtgttat gggtgccgac atcccacaga
     1261 ctagacaaat aaatgggttc tgctgagtgt agtttgagct gtggagcaag acgaaggttt
     1321 tgcagacact cacgtgaatt aaatattttt tgtctata
//
LOCUS       KCVB01000473            1404 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22297pri ultra-conserved element locus
            uce-550, sequence.
ACCESSION   KCVB01000473
VERSION     KCVB01000473.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22297pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1404
                     /note="ultra-conserved element locus uce-550"
BASE COUNT          394 a          286 c          278 g          446 t
ORIGIN      
        1 cactaaatcc tacaaactga acctttaagc acattacttg acaaaatagg aaagcacttc
       61 agaaaactaa gaaactgtct tttaatttct ttatcactga ttgatcaact tactacttta
      121 atattagtta gcccccacat tggataaaag ctaggaggga gttgttccac tccttaaatt
      181 gaatctgagg tagtttgcat atgctggtcc ctcgcacaaa cctcatttca tctctgagca
      241 ccagcaggta tcaaactttc tgagaattga acagcccgac cctgaaggca tccctttgct
      301 cagtgtcttc tttccatttc tagattccct ctttacaagc ttaaatgtct acttaatatg
      361 ctttaatgcc tgctctttag agccagggag cacttcatca cttttataca cctttatata
      421 acacactgtt aactagcaat gccttatgga acagcttgtc ttgcagctgg aaggcgcctc
      481 catggcatga tggaggtacc tgttcaccat tcacaatgag acagtaaaat gggaatagag
      541 gcctctgcat actgctgctg tctatatcag tcaacgatga atattcatga atcacagtgt
      601 catgacatgc tctgtaattg gcagcaacat tagtaatgac actgctggta tcaagtccct
      661 gcatattcat gtggtggggc aatatttcct cgtggtgctg tgtttttttc tctcctcctg
      721 aaagtgaaaa tgtattctga agctgcaggt gtccctggtg ccacaatcaa ttgctggctg
      781 gattgtgccc cagtgaaaat cagacaaacc attagtttct ttgtcaatct ctttgattaa
      841 taatatatct gaattatgaa aggttgggag gactctaact agtgtaaagt gaatctcttt
      901 gtaatagtta tgaatgcacg ccttaaggac tcctatatac catgtcgttg ccaccagcta
      961 aagggctttg aaaatatcac tggtaaaatg tattgatgac ttttacctta aaagtaacag
     1021 caaattattg aattacataa gtttacagtt tattttttat ccaaggtgtt tgaggtatga
     1081 gtagatttag cagcctgcac ctacataagc ctggtcatcg aggttacatg ctgatagtgg
     1141 gcacacattt ctttttgtac aggaattttc tgcgactctg tttcggcccc actaaagtgt
     1201 cccagaatgg atgatgaggg aattgggtaa agctttgcct aagctgcagc tttctcctcc
     1261 aagcagctcg atgattttct actcgtggct tcggtttagt gcagtgtatt atttaaactg
     1321 tggtgtaagg aggcaaatct ggttctagac cagtaggagg gacagtattg agatgtttac
     1381 attgtttaca acaaagtaaa taca
//
LOCUS       KCVB01000474            1561 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22341pri ultra-conserved element locus
            uce-181, sequence.
ACCESSION   KCVB01000474
VERSION     KCVB01000474.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22341pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1561
                     /note="ultra-conserved element locus uce-181"
BASE COUNT          486 a          297 c          296 g          482 t
ORIGIN      
        1 cagcatttta catggctgct tgggttatat ttagtctgca gtctttagtc tcttttttaa
       61 ttagatatat atgcatataa attataagaa aataaaaaaa tgtgttatta atgttgttgt
      121 actttaaaca taaaacctca gacctcatct ctaaatttat ctgagtttga aacatttgta
      181 acaactgttt tacattttct ataacaccat aaaaaacaaa tgcccggata aacatattac
      241 tgtcagcatg atatgtaatt ttatcaggaa ggtcatttct gtttagaaag acataagact
      301 ttatcactcc caaaagtctc aaaatacaat atttaatttt tctcaaccaa gtgcaaataa
      361 ttgttaaaaa ttgtgttttc ctatctcagc tgtacagtaa tagggaacat gtagtagatt
      421 ggtgactagc ctgtagggaa acacttattt ttctttatta atgggactca actgtaagag
      481 aaaaaacctt ccagtattac atttaggata gatacgtcaa agaagacatt gcacatggca
      541 gctgttatat gtaaccacct ctgttttgct gctgcgctcc attcaggtga agatgcctct
      601 ctgcccctca gcagtggtgg cagttatcag attctggtca acaatgtctt ctacttcacc
      661 caacgggtgg tggacaagct gtggcagggc atgttcaaca aggactccaa gctggtggtg
      721 gacttcattg tgcagctcat aggacaggta cagaccaaac cacctttaaa aacatcttaa
      781 tttccaggat ggactgatta accttaatgg taaaagaatt catatgtgtc caattttaaa
      841 aaaggaaaaa gttttcatat ttcactgaca aagaatccaa aaccagaaag gggcagcaaa
      901 acaccgcttc tcagcctggt cttaagttaa tctttaaggt ctcgtagaag attcctactt
      961 agaataatga gttaaagggc tgcaatttac tgaaaataaa ctgaattttc cagcttgttt
     1021 ctccagcagt gtactgtttc ccttcctgtg cctgcagaat cttgcttgag gcacagcaca
     1081 caggacagca agtctgtctg catttacaat aaaactatgt ttggttatgt gcaagagtca
     1141 aattatagca gataacgtta tcacgcccag aaaaaaagca ctagcatttc gctggtgcaa
     1201 cttgtaagca gcctgcatca tcagactgga cactttttct ccagagcagc cgaaggcaaa
     1261 cagacggcgt ctgtgtgctg cctgcactgg atggaggaat aaaactgtcc ttctgtgagt
     1321 cctcttccag ttaatgtaac cagtgaacac actcaggatt aaattagata agcaacatcc
     1381 atctgactgc aggcctcatt tattcatttt agccgtgaag gcacataaat tagatatggc
     1441 tgttagtcca ttaagtgtct gactgactga cagacaggtg tgtgtttggt tgatatgttg
     1501 catacgtgga gtctgatgca tgtaatgttt ttctaaatat ttactgtcta taggattaaa
     1561 t
//
LOCUS       KCVB01000475            1734 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22384pri ultra-conserved element locus
            uce-515, sequence.
ACCESSION   KCVB01000475
VERSION     KCVB01000475.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22384pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1734
                     /note="ultra-conserved element locus uce-515"
BASE COUNT          444 a          382 c          368 g          540 t
ORIGIN      
        1 tgtgagtctc cgaggagcag ctcgtctgga gattctgccg tcgacggcct tcccttgaaa
       61 acctcagtgg aagcctggtt tctctcaggc gggaaaacgc cgcccgtgtg tttaaagaca
      121 acagtcgcac agtgaatacc tgcctcgtat cacgtcttac atgttaacaa aatacctcga
      181 attactatga aatactgtgt gtgtggtgta gcactgcata aaaaggtgga attagaaatt
      241 gaaactgaat tttatgtctc atgtaagagc gtgattcatg ccatgtagca ttaggtgaga
      301 taagcctgcg ggactcttga tcggtgcctt acagtaaatt ctgtttttga tgttaaactc
      361 tctgcattcg agcctcctct ctgccgcagc ggttgtcctg tgttcttttc gtccactcct
      421 gctttcctgc gatgccacaa ttaatttctg tttgacttta tctgcatgtc ggcctggtgg
      481 agtacagact aagatgctcc catcgcatcc tgagctgcgg ttggttccac tctgctcttt
      541 tgctccggtg acccctctag ttgcccgtaa tctcccccat cacacttcag gatagcagct
      601 cgtgacctgt ttccttttct tcctcctctc tgttttttta tttattcatt aacttaacac
      661 agtatcgtgt ttgtaccctc ctgtgaacgc gtgtgatgtg aaatgaaagg gatattatat
      721 atattttttt aaatctgtca ttttctttct atctcccata tgacttctta atccatctga
      781 atgcatagaa gataatgttt tgggcgaaat aacataagag gaaagataga ctcccaagga
      841 ccaatatctt tattgcttgg aggataaacg gattatgtca gccattttct gctgctgaca
      901 gcttttctta ttaaccgatt ttatttttaa tctgccaagg aagaaatgcg gttttatgtc
      961 acaataaaac gctggtcaca tctaatgctg ccctcaaaca aacagatttc ttaaagcctt
     1021 gctgggaaat ttgaaataac tttaaagagg ggttttgggg aaaagggtgg tggtggtggt
     1081 ggtggaaagg gagggagggg tcttcatcca caagcttgtt cgactgagga gtaaaagatt
     1141 tttaaagtcc ttcacagttg tattcttctt ttaatattta tttctttatt ttgctgatct
     1201 ccgtagaaaa aaaggcttcc atcatgacaa aatggcttgt gtgtgggctg cctgctgtgt
     1261 ccattcatgc agcttgcatt aggataatac aaagaatacc ctgcatcctt cacttacacc
     1321 aataagtgtg tgtaagaagg cagctgcaaa gcttaaaaaa aacccaaaca aacaaaaaaa
     1381 ccctccaaca ttctgattac ttttatttat ttcttcaccg tccctaaaat cagagccact
     1441 atctcaggtg ggtcccgttt agcttcttgc actctcttaa gtgtgtgcag gagattctgg
     1501 ggatcatcta aaatgaaacc atttggccag atttatatgg aggggtgttg ggtgtgtggt
     1561 gccagggaag ctatcattaa cctcagggct ttagcgctcc ctgctctctc atttcatgct
     1621 cgtgatctga gggtgtgaat tagccatcgc cccgggcaac ccaacttagt taccggctgg
     1681 acaacatgaa agcaatacac agagctgcat aaagaatcct ctcaaagccc ccca
//
LOCUS       KCVB01000476            1608 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22474pri ultra-conserved element locus
            uce-238, sequence.
ACCESSION   KCVB01000476
VERSION     KCVB01000476.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22474pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1608
                     /note="ultra-conserved element locus uce-238"
BASE COUNT          430 a          406 c          385 g          387 t
ORIGIN      
        1 catgggggaa aaaaaagatc tttggataat gtaacagaag ccaaattcaa atttaatgac
       61 aacatacagc cgaaaacatg aagcggacca cttggcacgc tctcagcatc aagtttgacc
      121 ctttggaaac acaagacaac tgtgccaaga gaggtgatgt catttgaatt aggctaatta
      181 tcccttaaag agtctggtaa tactttgaca caatgacacg gtgtctgaat gtgtgcttga
      241 tttaaaatta ggcccgacca ccagagaatt aaatgcaatt tcagaacttt tttgttgtga
      301 agatgaggtt attagaataa tccagctcac cgagttcaca aagtctgctc atgtggtgcg
      361 ggttgcagca aacgagctct gggttgattt tcccgtagga ttcacagcaa gacagcctct
      421 tcagttccga ggaatgcctg aggtccggcc acctgaacac tttgtagagc agcatgggga
      481 gagagtaaga ctgttgaccc actttggcgt ccactttgct gggcaagagc aagcaggggc
      541 ttcgggcacc ccccttggac tccaccgcct gcaaaagcac ctctaattgt ttctctttga
      601 tctttttcag tatcgagtgg gtcaacgcct tcaattcagc ctccgacccg gcgttggact
      661 tggccacctt tgtcgttttg cccatgcagc agcccccgga gccatgcgtc cctctatccg
      721 cctccccgtc gccctccacg ggcgcacggc tcctccagag tcgccggacg agccccgatc
      781 gtttggtcct aaacatgcgg gacgaaaaga gggtttcccg gctaaaaaac gagcatggtg
      841 tccgcaaatc atgaagattc cgggatccga gtccaggcaa tgacaagcag cctgagaaga
      901 tacggtggaa aaactgggag ctggataagc agaggaaatc tgtagcatac gccagtctct
      961 gttgccgtct cctgcagact tgatcaacga aaagcagtcg aggttcacgg gacagtcagc
     1021 ttgtcctccg gggatgaata accatcactt ccacgcgttt gagaccagcc tataatactg
     1081 aatcttacag cttatacacc tctacagcgt ctggggaaat ctgctacaac acagcagcat
     1141 gtagcctgaa tgggttacaa ttattcactt ggtgatgtgc agcctagcgc cgtgaactga
     1201 cccctttaaa aacacattcc gtgccgtttt ttcagcattt aaacacagtc aaaacatttt
     1261 tctgtcgatg cgcattagct gtagtggaaa ttagacaaac tggggcctaa ttaagcctga
     1321 acgctatcac aattatccca tgaaaaccga agctcgccgc tgatcaacag cattaatcca
     1381 caaaatcctt gatccgactg cacagagtag agctctccac caggcttgcg gaggcttttt
     1441 tatccgttat cgtctgaata tgcctctcgt ctgcagagga tgtggtctgc tagcaagaat
     1501 gtgcctcgtc caaagtgcca gggagcaaat ccaggtctga atctctcacc cgagttgagt
     1561 tgcgtttgaa agcctgagag aaagcgaaga gagaccctcg ggttacgt
//
LOCUS       KCVB01000477            1202 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22513pri ultra-conserved element locus
            uce-280, sequence.
ACCESSION   KCVB01000477
VERSION     KCVB01000477.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22513pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1202
                     /note="ultra-conserved element locus uce-280"
BASE COUNT          420 a          185 c          273 g          324 t
ORIGIN      
        1 taaaacatta actgttacta cttttattca tcataatatc cgaagcggtc tggattatta
       61 ggtcactctt tggtctgact ctcttaagtt agaccaaagt gactgacagg ttgaactggg
      121 tgcagtctaa cgacacagtt gtcgtttgtg tcactaatta gcgaacagtc accacttgtg
      181 tggctttagc tgttggttgc ttaagtagtg tcatcaatag aaatgactcg gtaatcaatg
      241 ggcccattca gagctaatta agagagtcac actcaagttg tgacatttca caacatgaat
      301 gatttatctg cttataaaaa ataagacaat cataatatta aaaaatacag ttcagtacat
      361 gtgaattact ttaaggaaca atgacctgat ttttagtaaa gactttaata ataaactgaa
      421 aaatggatga aacctgattg atttcatgat ggtgattgaa acacaatcag agacgctttg
      481 cacccagtgg ttcactacct ttacagagaa aatgcatgtg tggatcttta tttcaaggtg
      541 aaagggaagt caaacactct tacaaattat agatcaaatc ataaatatat tgactggtga
      601 ggtttaaaaa aaagcaacaa caaagtcaat aaataaagtc attaccaggg cttagaacag
      661 aggcacccga ttttctcttt tatcctgcaa caacaaaaag aagaagaaga cagaggagga
      721 agaaataaca aaaaaaagag agagagagag aaagaaagaa ttgcacagag gcttttcttt
      781 tctttaaaat gctgcattag gtcttttcct gcagtcagtt ttcatagttc aaagctggca
      841 gttcaataaa tagctggaac tggcacatct ctgttcttgc aaaaagaaat caatgttaat
      901 cctattaccg gcagttatca cctagcccag aaacggtggc aagtaaggaa aaaaaaaaaa
      961 gaaaaaaaac tgagatgtca aagcgggaag gagagagcga gcgagagaaa gggagagaga
     1021 gagcgagtca cccttcattc actttcaaac acagttttga taatttactt ctgtagaaga
     1081 agaagaagaa gaagaagggt ggcgggggga tgggaggagg ggagtgagct tcagttacta
     1141 ggaaatgcat gtgtgtgtgt gtggctgtgt gtgtgtggct gtgtgtgtgt ggctgtgtgt
     1201 gt
//
LOCUS       KCVB01000478            1671 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22572pri ultra-conserved element locus
            uce-614, sequence.
ACCESSION   KCVB01000478
VERSION     KCVB01000478.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22572pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1671
                     /note="ultra-conserved element locus uce-614"
BASE COUNT          522 a          324 c          351 g          474 t
ORIGIN      
        1 actcaatatg ttcttgatct gatgaaaata tttgtagtga caaaagatta caagaacaac
       61 aaagtaccat ctcactttcc ttatctgaca aacattaggt tgtgttgttc tcgatgttgt
      121 cgggaattaa cagcacttaa agctggatgc atgacaagtc tgcaaaaaac aaccatctta
      181 gcaaagacac atgacatgca tccttcagtt attatgaaga aacctcgctt ttaatctgtt
      241 atgatcgcta tcaataaaga agaaaaacat gtttctctga ccattagtgt gtgtagtgtg
      301 ttagtgcatg cacagtgcag cttgtggcct caggctgtgt gcttagtgag gattgtaata
      361 ggacagcttt aaagggggat taatgcagta tggccgaatg agaaaaacac ttcctgtaaa
      421 acaaacacat gcagtaatta atatgacaaa tgcagtaaaa agggtcagaa acatatactg
      481 tgtattaaag actgacttta tttatgtgta agatacaaca tttcaatagc tatcatgact
      541 ttcacctgga aaatgctatt ttttacgctc agcctgttat cttaaatgtt ggtctttaaa
      601 gagttagttt ctctctcctt tcatcttcaa gtatgtcaag ggatcataag ttcacattta
      661 acttccaata ttctaatata ttcttatatc ttttctaata gctcagcctt ttttagccac
      721 ctcccctctt agcctctgat gttccctctt tgcatgataa acagcttttc agccctcaga
      781 atatgtatgt ggcccatatt tattcatatg tgccgtatat gagagcagac aattctgctt
      841 tgggtgtggg agaggaaata tataaataag gaagtaaata aacagtcgaa ataaacagcc
      901 agagtaatga ggttaactat ttaggttttt ttgatattga tatttgcagg cagagctaca
      961 ggagagggca aaattaatga cagcagacat aatgccattg ggatacttag gagtgtgtcc
     1021 aatactttaa gtaatagcgt taatggcgaa tttgaaggaa tttaattttc gacatcaaac
     1081 agcagcgcct ttagaaagcc accaccctct cgcctccaca tgtttggggc tggagagctt
     1141 ctgaactcgc ctcctctcag agggaggaag aggaggaaat catctctggg gtggcaaagc
     1201 atgcagggag gcaggtggct aaaaacttcc aagcagaaca gcctatcgac tcgcagggtg
     1261 acgcttgtta ggctcaggcc cagaggaggg agcatggcca gtaagtaagg ttattgggaa
     1321 ataattagcc tagcccagga atcagcaggg atagcaaagt cctctggggt agaaacgcca
     1381 gtgcccctgg ccttgtttcc acagctaatt aactttgaag agagcctatt aataaggttg
     1441 tcttcataga aaaagcctga aactatatgc agaaggaact ttttggtgtg gcggaggctg
     1501 gaattcaatt tatagatgaa acacaaagaa acacacaagc ctgtgcaagt aattgaaaat
     1561 gagaaggtct ttacgggtat ttctgcgctt ttttgtgcaa agttctctct ctacacaagt
     1621 cacacacaca cacacacaca cacactagtc ctcaccacct cccactcaga g
//
LOCUS       KCVB01000479            1488 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22612pri ultra-conserved element locus
            uce-139, sequence.
ACCESSION   KCVB01000479
VERSION     KCVB01000479.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22612pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1488
                     /note="ultra-conserved element locus uce-139"
BASE COUNT          449 a          312 c          368 g          359 t
ORIGIN      
        1 acgacgacga caaatcaccc atgacaacac agtcattttc agctttacaa tatgcatata
       61 ttcatgcata tatagtgtgt atttcccctt gcatacaggc gagtggttgg ggactatata
      121 gagagccttt agttatatca actccctctg cagagactaa aactgattat taagagattg
      181 gtacaaatga gagcaaacat aattcacaga caggctgagg ggcgagaaga cagggactga
      241 ttctgtgcag ccttgtaaac agaggaggcc agacagccag agaggatgat gggtaacaga
      301 catccactta aagcagcaaa gataggacag attacaggag gtggaggctg ggcgggagtg
      361 aggaaaagaa gaggtggggg acctttttct tccagattac caatcagagt attcaaagca
      421 gctgtatcag ctcacaactt cgcctcgtct gcttccagca agtttggcgg aacatcagtt
      481 ctgacgctga aaatgaagtt aaagttatcg acgcgttcct gctcgagagg aaaactactc
      541 aaaatgtaag aaacggtgga ctctcagcaa tgtaacacag cgtcttatag taaagcaacc
      601 tatgggatga gtgagtgttg gagtgggatg agcaggaggg tgcaacagcc agatataagt
      661 cactggggct tcaaagacaa cagttgcccc ctcccactcc cacttctctc cagccccctc
      721 gaacagaccc ccccttgcgt cgttaatagc tgagaggacg aagaagctca gacgaataac
      781 tggattcgct cgcggatggt ctgccggcag attgggcagg tcttgagcgc ggcgctgcag
      841 tcgatgcagg aggcgtgtcc gcactggaag acaagcttga tgtggttgtc aatgcagatg
      901 gggcaggtga ttcgctcctc catctgtcgg tagcgagact gcagctgctc caacaggttg
      961 tgctgttccg agttctcggt gccggggcta cagtccactt cggtttggtc tgcagagggg
     1021 aaagagggtc atcaacatga atgatatcat ggtagcgaag aactgaatgc acagacaata
     1081 actgtcagtt aatgtctttt agatgtcaac agaagtcaca tttaagaagg ctgtgcagtt
     1141 ttgtgctgct ggttcaaaga gaataaggct ttatactata gcaactgacc aagaagatag
     1201 catgactcaa tgtatctgac aatttcattg gtgacttgtt cttgacacaa aagtgctttc
     1261 atctctgaca ccacaagact agtaatgcta agacagacac attgtgtcct atctgcacgg
     1321 tgcatgtatg actgtaaagt cattaaagta actcatgaat aaacattaca ggaacaaaag
     1381 aatatctttg tccccaacat gaatatatac agaagagctg tactgttctg actgaaaaaa
     1441 gttttcatta ataactatgt tcaatcaacc atcctagcta aaaagaat
//
LOCUS       KCVB01000480            1469 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22646pri ultra-conserved element locus
            uce-709, sequence.
ACCESSION   KCVB01000480
VERSION     KCVB01000480.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22646pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1469
                     /note="ultra-conserved element locus uce-709"
BASE COUNT          465 a          328 c          293 g          383 t
ORIGIN      
        1 gtcagcatgt aacagtagat tgtgacagta gtatgttatc gatggagaac atcacatggg
       61 gctgacgtgg tgtgcagcaa aggatggcgc aggggaaggc ggtagtggtg gacagtgatt
      121 ggttgatttg gttggtcagg tgactgaggg gggttatgga cagtcgagtt gagtcagatc
      181 acatgatgag ggtttgcagc tataacgtca ggaggttcca tcaggaggta tggaagcctg
      241 acaggtttta gcttctggtg gacactggac agaacaccca ccctgaaccc caccctcaca
      301 gagccagcga gcacatacac tcacacacac tcacacacac acacacacac acacacacac
      361 aaccaaacac acagaaacag acatgcacta atacacaagt gagcagacag atgcagggac
      421 gcacacacat ttacccacat ttatcatatt ttgcatagac acacacatat aaacacacac
      481 atttgttaac taggactcac gcgtacattc atgtcaaaca cacattactc ccacatttta
      541 cacaaaccca catacacact ccttgactca cacaaacaca cacacacaca ctcttgccca
      601 cccccagact tgcgatgtgt tactgtgatg atgcagagtg tggtggaagg tgaagaataa
      661 gacaacggaa gacaggtgat gctaaaatga acatttataa tgactgctgt tgctgtcgca
      721 aaataagaat ccttaaaaaa agacctcata tatattagca ctagattgcc cacttcactg
      781 gtaatccaca agtcaacaaa cacaactcac taggtttttt ttctctttac aaacacaatg
      841 ctacaattac attaaatatt caatactctt taagtcagtt aatgaatgtt gttaaaagca
      901 accactaaaa tactaaaaat aaaaacaaaa caatactaat attaattcac ctttttccag
      961 tcgttccctt tttcttgttg cgttgttatt tattaatagt atagtaataa taatagtctg
     1021 agaaataaat agctcatgtg gtgttctcag ggcaatgggg caggcagcat aaatcaagag
     1081 tggagctcag agcaatgcaa tgaaatggtg gagtctaatc tcacgctgtc acgttaatac
     1141 tttcaaaatg tctctgatgt ccaggcgaaa tatggagata tgccacaagt ttggttctgc
     1201 aggcagcagg atggttgagc gccccctgca gaggttaatc atctctaaac tatcattcta
     1261 tgttatgtaa actaggactg tattgcacaa aagttagcag ctcagcttcc tcgtttttgt
     1321 agcccgggaa gagctcagtt tctttttttg ttaaattttc ttccgtccac ctccctgggt
     1381 ctttctccaa cctggaggag ccgagtcgag caattttcca cgatgatatg tctctaacac
     1441 gaccaagcat tttcaataac tatggcttg
//
LOCUS       KCVB01000481            1440 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22685pri ultra-conserved element locus
            uce-1053, sequence.
ACCESSION   KCVB01000481
VERSION     KCVB01000481.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22685pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1440
                     /note="ultra-conserved element locus uce-1053"
BASE COUNT          415 a          335 c          312 g          378 t
ORIGIN      
        1 tgcaggtgtt tgtccaacag gtaacagact acagggagaa gcactgctgc tcttgcagtg
       61 ataattgcgt gcccttatta gtattaacac agtgcacacc tttgccgaca caaactccac
      121 taaaagtctc tacagctggt aaagccgact gacagctgat ccagctgtga tcagctgttg
      181 ctgtcataag taacggacgt cccttcatgt gacgcttcag tcaaaaggac atcttaattt
      241 ctcaaaaaac atacttcctc ttttcctcaa ggattttcaa tgcaaggaaa agacacaagg
      301 gagggaaatg tggatgtaat ttaacagaaa tggggatgca agagaagtag catttttact
      361 ttcttctact ttgcacttat gcatcttgta gcagtaaaat acttaaataa acttatactt
      421 tctagaaaac aaatgcatac acacacacat ccatcctgtc aattatagat ccagttatta
      481 tacaatgtat ttctgagtgc ccttttgtgt aacgtccagg atctgctggc agggcatgaa
      541 ccctgagatg aacatgagac tacaacctgt tgtagtctag ctgagctgaa cgcctcataa
      601 gtggagcttt tgctttttta ttgatctctt tgtttctcat agctggaagg gcacaatatt
      661 ttctccaacc tgaattccag tgagtacgag caggtgttgg agatcatccg gaaggccatt
      721 atcgccacag acctcgccct gtacttcaac aaccatcagc agctgacaga gctgctgtcc
      781 tcgcatgcgc tggacttgaa caaccaatca cacaggtcag gtcacgctga tttacaccgg
      841 tacacaggtc aggtcaggat cacatcgggt cttgttgcat cacacagaga agcctagacc
      901 tggcacaaat aaacggcaca ttgagtctct ccacacggct ataacgagcc tttgctcccc
      961 tccagggacc gtgtgattgg tctgatgatg acagcatgtg acctgtgttc cgtaaccaag
     1021 aaatggcaaa tcacacgact cacagccaac gacatctatg ctgagttctg ggctgaggta
     1081 cagtgggata ctgctggagc tgaaacagtt ttagtgtctc gaccatgcct tcagtgtaac
     1141 agtcatacct ctgttcttac tagtcctaca atttgcaagg cacaaaatag tgtcgtccag
     1201 tgatcagaag gtttgccaca gtgtgcagat atcaaaggaa atttctctct ttattttgtt
     1261 cccccacagg gagatgagat gaagaagatt gggatgcagc cgatccctat gatggacagg
     1321 gacaagaagg acgaggtgcc ccaaggccaa gtaagacctg tgtaactcag ttattacaca
     1381 gtttgtgaat tttaccaaag tcaccaaaaa gttggtgccc gaatcacaga aacaaacctt
//
LOCUS       KCVB01000482            1441 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22729pri ultra-conserved element locus
            uce-867, sequence.
ACCESSION   KCVB01000482
VERSION     KCVB01000482.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22729pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1441
                     /note="ultra-conserved element locus uce-867"
BASE COUNT          476 a          273 c          251 g          441 t
ORIGIN      
        1 agcgtgtcta taaacaccat tagctcataa tttaaggctc atagttaaga ttgagcgagg
       61 gttatcttaa gggaaaaaca ggtctattta tggagtgcag atgatcaaag aaacattatt
      121 tcctgctttt cacttcatct tcaatattca tgagtaaatc ctctcttttt ttatcaacaa
      181 tgctgtctgt cctttatctt tggataagtg taaacattta aaagatgatc aattaaatat
      241 actaggtgtt ctcagcggca gaggtttaat ctgcacagta aaggatctaa atcacacgtc
      301 acgctccgct ctggatctcg ctgtagaaac aaatgcccaa tttcccaccg acttctaaag
      361 gaaaaatgcc aaaaaaataa tgacagactt caaagccaag atgaggcttc tgaatatatt
      421 cttaaacaaa tttattttac accttaaaac aaaaaaaaaa ggatagggat tagggatgtg
      481 gtacattggg aatgtattga caactgtagt ttacattttt tggtgtaagc tcagagaaaa
      541 atgttaatac agtcataata attgcacttc ataaatctag actgaggcca gtatttcccc
      601 acaacaaagc tccgtctccc tctctctcca gttactaaat tcaatttacc attgaaattt
      661 gatggacaaa agaaatgcta atttaagacc agaggactgc tgcactgatc caatttttct
      721 ctttcacttg agtcccacaa ggcaatacac cattgcagta atttaagtgc agtacattca
      781 agggaatctc acagtcataa taaatatttc actgctctga atcatcaagt taagactggc
      841 ctgggctaaa tgtgtcccaa ccaggggcag aaatggacaa ggagaggcag tgagcctcgt
      901 ctcatttcat ccgatgccag aggacaggac atgattgttt tcattttgtt tttgtttgca
      961 ggattgggat tagtgaatag tacccctaaa atatgacttt cttctgttgc cacctctttc
     1021 cgtcctctga taacatttga cagaagcagg gtttgttgtt gtgtaacaac aggagtcatg
     1081 tgagaacttg atctctactg tagctccaga gaatatccaa agcctccaat aaaagagagc
     1141 aaaatttgca aaatatctcc cgtaaattac tcagatatga tgtttatggg actggtgaat
     1201 cacattttca gtcttggtgg taaaaatctt gtaatgacac aattgaataa taataataat
     1261 taaaaaaaaa agtctgagga ttttgaagaa cacaaactaa aaagcacaca cgcgcatgca
     1321 ctcagccaca tacacgcata aacatacagg ttatgtagat aacctcccaa tcacattctt
     1381 cattgtaaca tttctttttg ttgtattttt ttgcgacatt ttctttttaa ataagtattt
     1441 c
//
LOCUS       KCVB01000483            1921 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22778pri ultra-conserved element locus
            uce-65, sequence.
ACCESSION   KCVB01000483
VERSION     KCVB01000483.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22778pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1921
                     /note="ultra-conserved element locus uce-65"
BASE COUNT          510 a          398 c          478 g          535 t
ORIGIN      
        1 gtgtgcagga ttgcatgctt tcattcttgc gtgtgacaga cataaaacag ggcagagagc
       61 ctctgcagag acagacagta gtcagtgcct gcagcaagct cagagctcac agggtaatgt
      121 atactcgctc ctcgccgtac cagagagaag gtgacaccag tcaatcccat tctgacaggg
      181 cagctgagat aatagccggc tttgattccc agcaataact cctgccaatc aaaatgaaag
      241 gcatcgtgat tgcagggaac cacgagtgtg tgtatgtgtg cgagtggtag gagcttacag
      301 tacgctacag cgtcgcgaag gtaattggat gcaaaatgtg gttatgtatt ttaaaggcat
      361 tgtcgtgctt tgtgttatca ctgaagagaa aacacacaag aggtggatgg aaggagaaac
      421 atgtgcaggg cttttacaga tcatctgcgc tatgataatg tctgcagggg gtgagggtgt
      481 gtgtgggatg aatgtgtgag cgtcttggcc catttttttg actgtttgct gtcaggggtc
      541 agcacgtggt ttgtgtgtgt gtgtgtgcac gcaaactggt gcgtgcgtgt gcatgcatgc
      601 ggggaagtag gagggtgagc gcaaggccag gggaaggagt ctttcaggtg gaataaaagc
      661 atcactgcag tggtagaaag gtcagctgta aacttgtatc agactagtgg tgatcaccct
      721 gtgctgcaga tctctgaggt attgagcagg cacgctctaa tggctttgat atatggacta
      781 caggactccc tgaagaggtc aataccatag cactttgtct ctatgagcaa tgaccctctc
      841 aagctccatt tagaagcttt atatctgcag gaaataggct ggaaaggcct caggcagggc
      901 atggtgaaaa gagaactaat atgaatgaag tattttctaa tcacttcttc tgtgctccaa
      961 tcgttgcccc aatcaatcat tagttatggc ggtataagca tattgtgccg gacgttatca
     1021 gacgtagatt tttgatgtgg ttgagagaag aacaagggaa tctgagctga atagagctga
     1081 aagagctgat ttactattac tcaatttgga agggcactga cactggaaag actggtgtct
     1141 gagagcgttt catcattact ttagatagaa tctggtgagt cacggtgttt aatgagagtg
     1201 acagacgttg ggctcggcta caaatttgtt gatttatcca caacttgtca atggttatct
     1261 gtaaagggga gttaaatccc acaatagctg tttagtttcg tttattaatc ctttctgaaa
     1321 tctctttgtg tctgcggaaa ctgtcacaca aacacaacca caccagaaaa acgtcacaat
     1381 taaagactaa agacttttaa ctacaaagtg ctccgcaaca tcttcaacca acaatcaaga
     1441 gagctgcgag ctgcggcagc tagaggaaac agaaaccaca actgacagaa cagagagccg
     1501 ctcttttatt ttagccaatt tctcttcaaa cacatccgga cataatggcc accatcgtgg
     1561 tcccttctga gcgtattgat ttggaactaa cctttggtgt gaaatatgaa agaagccaaa
     1621 tactgtttat gaatgtctgc tgtaattgga atgagcagat gttgcaatat tgttccgctg
     1681 tgttcaataa cagacttctt gtgctatttt tcttctctac cctgtctgtt caatccctcg
     1741 ttccttttca ttttcctctg ctggctcatc cctctttctc cctcgctgat cctttctttc
     1801 cttcccctcc catctttctc acatgcagac tctgtgctct gtgctgtggc tgtgctgagt
     1861 gactaagtgg agccctacaa gccatggagg agatggacag taaaccctac cagccgttgt
     1921 c
//
LOCUS       KCVB01000484            1498 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22818pri ultra-conserved element locus
            uce-822, sequence.
ACCESSION   KCVB01000484
VERSION     KCVB01000484.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22818pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1498
                     /note="ultra-conserved element locus uce-822"
BASE COUNT          479 a          296 c          312 g          411 t
ORIGIN      
        1 tttctctata gatgatgaaa gagtgtatac tgggggtgga gtgacactgt tacgaggcaa
       61 atgcagtaga gggcgttaca gtaggacccc ctcacagggg attggtttgc aggatggcaa
      121 gagtaacaaa tggtttaggt tagggctgtc ctgcacacca atccacttga gggctgacca
      181 ttacgttaga aatcaaaagt aaataaacat cttgtcattg gtgtattacc taataaactg
      241 acattacaca catttcactg gatcaccaca cacgcacgca ctgctgtggt aaggaactta
      301 atctttgaaa aaaacaaaac aaatgtgatg agtcccattt tgaactcatt ttgactacaa
      361 aaagtcccgc tgtcgtaaat agtataaaag gagcaaagat ttaaatacaa agtttgcttc
      421 ctttgtatgg agttgtcttt tctctgcaga gaaacaggaa agacaggctt tccaatatct
      481 cctgactgtc tcacttctct ctgaaaggca catcttaagt ctgactaaag cgtacactcg
      541 tctctcccct agggtgcagt ggcagtggtg cagaaaaagt gaactgcaga ggaccccact
      601 gtttagcaaa cactcctact gaggggagga attaattatt cagagccaag gaccaaggca
      661 gccccatttg attgcaccac aaaagaaagg tcagagcttt tcatatgagg aacctcttgg
      721 ttctgcaccc tgtcagttaa ggggacatgt acagtatata agactaaacg gcagactggc
      781 taacagctca attaaaaggg caaggatgtc taggtgcgga tgtgttcacc agacatgtga
      841 caaaaagcgg ctaaaacatg ctttcacaaa aaaggttcgg atggtctgca agaactaaat
      901 atatttgaat cacacgaagc tctgagatgc cgaaccaaca agaaaggaat taagataccg
      961 ctggagagaa agattaaaca tttccacagc cactgaaagt agtttttgtt caaaactgac
     1021 caaatcgttt catttctcct tcctcgcaag ttagcaaatg atttattact gtatttgaat
     1081 agttaattgg caaaaatgga cttcatcaaa ttcataacat cttatttaat cttgagaaaa
     1141 attgctttct aagaactaaa ttgttaggtt taaaattttg caaatattgt atagactcct
     1201 gtggcaattt ctatagatgg acaaagtttg atgtcaggct ccattctaga agtgtgatca
     1261 tctgcataat cctatcaaat ttcactgtga aaggccaaag cttctttttt tttcagccat
     1321 ggtgcaaatg cctgattttc gtcagtcctt tctcccggct ctgagctcac aatgtctact
     1381 ttgcagagca gatcataaac gtacagtaaa agtgcaacaa aaaacagctg aaggatcctc
     1441 agggcttatt gagtaaatct gccgttaaag tagaatactg ctgaggcctc gtggcatt
//
LOCUS       KCVB01000485            1500 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22859pri ultra-conserved element locus
            uce-98, sequence.
ACCESSION   KCVB01000485
VERSION     KCVB01000485.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22859pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1500
                     /note="ultra-conserved element locus uce-98"
BASE COUNT          466 a          256 c          294 g          484 t
ORIGIN      
        1 gtcacaagca aacagctatt tctttgtttt tagggtcaga agccacaaaa tgtataaaac
       61 agtgtcaagt gtcagaacaa aacatatata acatagaact gattcatgtc tttctttgcc
      121 cacaacttgt ttgttgtgat tccaacttta catcatttaa aaatgttcct ccatggagtg
      181 ataatggcct caaaagagga ggtttgagtt ttttgttttt tttttctttc tctattatcg
      241 ggtttataaa ataacttggt caactttccg ctagaagaga atggcatgtt aaatctgaat
      301 aaatgttgtt tttgtaaatc tgaatttaat atgggctaca ggtattggat tttgtctgct
      361 ggcagggagc cacacacaat agcaggcaca ggtgttgacc aaaaaaaata aataaataaa
      421 taaaaaaaag agaaagaagg gaaaggcaga aagtgatttg aattatcatt gtggagtatt
      481 tggcatctct gcaatgaaaa gagctttaaa tagagccaca aagttttatg taaaattgat
      541 gcatggatat ttccaaaatt tatgtttcta catttctttt ttttctctct ctgctgaatg
      601 tttttactaa ctctcgattt caaaatgcgg cgcttgttga tcaagagtac agatatcttt
      661 gacttgagcg tgggataatg agagaaatta gcacaaatgc tctaagtaat agttcctgat
      721 ttttttcact cacaaattat ttttcaactt gtttttgtat ctatgcataa attaaaaaat
      781 ctgctgaaat ttattattca tctgagatgt gattaatatg caaaattatg caaattaaga
      841 tgcaattagg ctccaattct ctagtggaat tttccattat aatttaatac aaagtaacag
      901 agtaattggt gtcacgtgta attacggtta ctcaagtgca ctttcaggca tgcaaatgat
      961 tcgctatatg ttggttatcc tatgcaaatg taccatgtgg tgttttaagt tggggaaaac
     1021 acaatgggtg cgtggttacc cagtggggtt ggaacaaggt aaagacacca gggtgtattt
     1081 tttaaaatga gaagagttgg aaaactttca gcgctcagat tatcaccagt tgagacttcc
     1141 agttactttt tcttcttttt tgaatttttg atgaattgtg gagaggcgca gactttcaca
     1201 catcaccaat cgattaatga gagaaaactg ttttttggat gtgactcggg actcgtttcc
     1261 aacacgaaac tgttgcttca gttgacacaa atcacaggaa acttcacacc ccgacacctc
     1321 cacccctcct cggttcagtc agagcttctt gcgtcttcat tacaactgca ccctgtttga
     1381 tcaatgacac tgcaaatgtc acaagcaatc acaaaacatt atgcgcgcgg gacaattacg
     1441 ccgcagctcg gtgtgacaaa ctttgcagtc agtggccggg cggagaggag aaaagcgatt
//
LOCUS       KCVB01000486            1676 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22904pri ultra-conserved element locus
            uce-408, sequence.
ACCESSION   KCVB01000486
VERSION     KCVB01000486.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22904pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1676
                     /note="ultra-conserved element locus uce-408"
BASE COUNT          508 a          354 c          347 g          467 t
ORIGIN      
        1 gaaaaaatcc tgatttctaa gaggctggaa ccatgaaatg tttgacattt ttgcataata
       61 aattactgaa aagttaaatc agttatgaaa acagtttaca gtttctgtca gtaaactaat
      121 caatttgtaa agtaatcatc agtgtcagaa atatgcagtg aagtaaacgt gtaatagttg
      181 aagtataaag tattatgaaa taaaaaaaat actcaaattg tagttgatta cttgagtaac
      241 tgtacatagt tactgtccat tactgcagaa taaacatata ttagtttagg taaataaccc
      301 aacagggaga taaaaaaata acatcaacac agggtaaaaa cccagtcatt ttaaatgtgt
      361 acaaatgagc tcacattaca gtgtttactt atacctttaa ctgcaagatt aaacatgtct
      421 ggctaataaa gttaacagcc aaacagtgtt aacacccaaa caaagttacc actctcacac
      481 ccacgctggg agtccaaagt cagccacaca agcatcaata ttttacattc cctcaagaat
      541 cctctttaaa gatgcaatca atgcctccta ttcattatcc atgtgtcttc taggtatggc
      601 ctttgtcatt aagcaataat tcacccgtcc acagtcccac attactccgc tgctccatac
      661 ttttacaaat gcctcggggc tataaatact aatgaaatct ctttgcttaa tttgattttg
      721 ctggtacact tgcagttatg gctgaacttt cccacgagag aggctgattg aacactttca
      781 agtccttatg gaagcaggaa aacattccaa agagctgggg agatcatttc cataattacc
      841 ccgattggca gcctaatgac cttctgtgac aataaaaaga tctcttgtta attccatttc
      901 tgtcgctgac attcaagaga ttcctatctt acatgtttga gggttcacaa gataacaagt
      961 gtactttttt ttttccccct ctcgttttca gcgagcaaaa gagcagcccc attcgggttc
     1021 gctcccccaa cgcactgcag tgatgtggct ctgctctgct gtgaaaggga tctgcttgtg
     1081 gctgcacagc tcgcccaccc tgtcccaaag ttggataatt tatgggcctg gggggagatt
     1141 ataaagatgg atctttgagg caatcaattc tcatgttgcg ttttgaatgg tgggatagaa
     1201 atgaagtgca gaataattaa aaagatcaca agtcccccct cgcatgtaat aatgagtggc
     1261 tccagaggca gtgggctccg ccacacagtg ccagaggaag cgcctttgat ttgactgaga
     1321 ggacacccgg ccccggtcca acacggcccc ctgcacaatg ggggggtttc accatcgcca
     1381 cacactgagg ctgcagtgca aatcacgctc tgtgaatgtt tgaggactgt gtgcttgtcc
     1441 agcagatcat aagcagatac tggagcagat acacaggaag ggggtgttct tgttgcttgg
     1501 atgtccaagt caggatgact cagagctggc atgaaaagtg atgggggcgg ttgtttggaa
     1561 tatgtttcac agtgaccaca atgctcagag tgttatggga tgttaaaata aagtccatcc
     1621 gtccattctt gcaagtgaat cagtgtcaaa atgtgccatc agccctttgc atggac
//
LOCUS       KCVB01000487            1755 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22944pri ultra-conserved element locus
            uce-1317, sequence.
ACCESSION   KCVB01000487
VERSION     KCVB01000487.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22944pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1755
                     /note="ultra-conserved element locus uce-1317"
BASE COUNT          575 a          325 c          331 g          524 t
ORIGIN      
        1 gaactgccat tccacctttt ttttcccatt gaactcttgt tccttgatcc tgcgctgtca
       61 aaactgcact gatgtttgta aaatgtcaaa ttattgccaa tataatggat gtggtggagt
      121 accgacggag gaaatgactg tttgcattga aggaggaaaa ggatgtggga catggttgtt
      181 cacatatgca aagtaaagag tgagtgcata cttcttcttt atcatacctg tatcagtaaa
      241 cagaaaatca accttgatct attaagccgg taatttatct atatctttcc atgtatttgt
      301 tgacttgtcg tcatttaaac gtagataaaa taatactgga taaaaggcct ccctatcatt
      361 tctttgcatc cagtgacatc aattggatgc ttttgctgtg tgcggcatat gataacctag
      421 cagagttgtc aatgaagacg gtctcttagt gccacagaat ctgactaatt acgtccatca
      481 aaagaaggaa tgaatgtcac ctatagaaac acttgcagct tacagataat tgcatacttc
      541 tgtctttgca gggtctacac catagaaatg gtcttatttg tgagatgcct ggtggagatg
      601 gcctacaatg caataagtga atgtggtagc taagcagagt agtagctgtg acatggcatt
      661 atgggaaatt acacttaccg tgagattgca taaaccactc accaagctgg aatccagctc
      721 cttaaattaa gagaacccat ttcacccatc agactgagca aggtaatgaa atagcagatg
      781 agaggtctca aatgtaattt tccatgtgga gttcttttgt gccgttatct cagagggtgc
      841 aataatgatt ctgtgaatga cttttgtgca ccccttaatg gcccacagta gttaaggcca
      901 cagtgtgtaa aactataatt accatgtgat ttcaaaactc aacgttttca gcttgttgta
      961 aagacatcct ggtccttgca taataataac agcaatgtca aaagtattca attttgagca
     1021 aaatgtaacc tgctatgaac atttacaatg actgacatta ctgaaattcc atttagtttt
     1081 tgtcattaaa acgaattaat taccttgtga aaagcatgtt tatggaataa agtctttgag
     1141 gaccttgaga tgcagctaca tggaactcgt gaaatacaat taaccaaaaa tctatggaaa
     1201 tactctttaa accgactcaa gtaaaaataa agaggtttac cactcaacat aaagctcagg
     1261 tatgcagccc ctgtttaaaa taaatcaaaa tcaactaaac agatgcagca atgaatgaga
     1321 gtcctagcct ttaattaaaa agaaaaaaat agatacattt ttgaagcaca ctcttaaact
     1381 gctgcaattt catttcagaa aagaaaatag ggttgaatat caaggaaatt tttaattacc
     1441 acaatcatgt tgaaaacatc tgcgaacaga tcacagcatg tcactaatct atgaaaaaat
     1501 gtcaggacaa aactattgat tctaggttgg atttttcttt aggtttcatg aataaatcta
     1561 cagatctgga gtacagatgg atgaataaac aaggttttca ataccaattt ccaaaggtcc
     1621 accggagagc ccagaaagct tacttatgct gccactgtta ttggcaatgg cctcttgttt
     1681 atttcatgcc tctttgaacc ggcaggctta ggtgccgtga atctgtctga tatcatccca
     1741 ctcaaaactg aggcc
//
LOCUS       KCVB01000488            1834 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 22982pri ultra-conserved element locus
            uce-325, sequence.
ACCESSION   KCVB01000488
VERSION     KCVB01000488.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="22982pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1834
                     /note="ultra-conserved element locus uce-325"
BASE COUNT          509 a          378 c          429 g          518 t
ORIGIN      
        1 catgtctttt cattagagat gccctcccag gattggtgta aagtgttttt gacataacga
       61 gtaaccatag atttcagatt tatacagact cacctgtcag tagcaataac tgaattctga
      121 gctcatgctt acctcctata aaagtatcat acctcagtta ttaaggaaga ggcctatcag
      181 tcattcataa tcatttctaa ctaccaagtg taaagctgag actttccagc tttccatctc
      241 tgtttctaat gtaagagtca ggaagtagaa gtgcggggag taaaacaaat tccaggtaat
      301 acagggattc ttctcccgtg aatcaggttg ctttatgcat gaggcagcac attagttcac
      361 catgatcact tatcagtgag tgacagctct gacttgtttg tatgcctgag atgtttttta
      421 tcttttagag agattattat gagttacctt tagctctgca cttgagcagg gagtttgagg
      481 gagcttaagc atctgcactg taaggacaat caaattgcct gcccttgcct gtcaggcctg
      541 atctcaggat tttttacttt gctcattata aaggtatttc cattttatta agttttccct
      601 ttttccaccc tttgtctctc tgggaaattt gcatacctct attccttggg aaaaaataat
      661 gacagcaatt tcttttcttt tctctccccc actagccgtg tgagtgcttc gtttagtggc
      721 acctgtgaaa gcgacaggcg cagagatgag aagtgaaaga gagcagttga ttaaatcatc
      781 atcaagtcgc agcacagaaa gtcaattctg cttgccgtct acaatgatat ccttttatcc
      841 taaagtaatg agctatggca cttttgcatc gggtaatacg atgagttcca ttagcatgtt
      901 ccatttgctt cacaagcaag ggcaagtcat tgcagccggc ttctatcttg gcaatcaaag
      961 ctattggctg cacagccagg gaacaaggga aaggagggag agcaaaaacg ggaacatgag
     1021 aaagaaaggc tagtggtgtt tttaatggat cacaacacag tggaaaacta aaaatgatgt
     1081 ccattatttg aaccccttgc atgtgtgttc ctaggattgt agtgtgcaca agcagagaag
     1141 cagctaatga gcgtcagtcg tattactctt gtcacattct gagaggcctg aatacatgga
     1201 agctgttgat ggctaatgct gctcatcaga tcttgtctgc tgctgcatta gtcagttctg
     1261 agtgggaggc cgcaagaggg agcgatacat ggccgacaca cctatttatt cacaagctag
     1321 actacatgtg tataaatgca cacagtctgc agatttttgt attacacaga cattcacatg
     1381 taatcagaaa ctgtactcat cttgcattac ctttatttat tcttcaagcg gacagtctat
     1441 agggctgctg gctctctgca cactgaatta aaatgggggg tagattgttg tgggtagtga
     1501 agaaagtggg cgagagagaa atggagagca gggaaatgtc tgaggagtgt ttgcccagtg
     1561 cgtttattgc ctctgcataa tttatgagct gctggagctg accatcatgg cagtgtgccg
     1621 caggcggccc ctggggccgt gtgggacagg cctgtaaatt ggatgctatg ctcctgctga
     1681 gctcgcaagg ccccttttta ccgtccagcc tcagaaacag gccagctatg agcccagcca
     1741 gctacctttg agctctgcag ggccccagat cagcaaaaaa caagagatgg aagcaaaaaa
     1801 ataggagaga aagaaaaaac ggcagggcag tcac
//
LOCUS       KCVB01000489            1301 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23027pri ultra-conserved element locus
            uce-435, sequence.
ACCESSION   KCVB01000489
VERSION     KCVB01000489.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23027pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1301
                     /note="ultra-conserved element locus uce-435"
BASE COUNT          362 a          254 c          281 g          404 t
ORIGIN      
        1 tgacctcaaa attgataatt ataccagggt aaaacacttg tagtacagac catactggat
       61 gaatatcttt tgacatttgc acgttgtttt accacatctg ttggctggta tttttgtcaa
      121 ttttaaaata tttttcatga tgataatttg agatttccaa tttttttcta ttggtacctg
      181 agtacatgaa ccattatgtg acaaatttac aaaagggata attcttgtgt agcctctctg
      241 tccactgaag cagagtggcc taatgcttgt ctgtgttagt ggaaatcaag catgtatgca
      301 tgagcaggca aagacaaggt ggaacgatgc cctggaggtc atccattggt tatttaccca
      361 gaggaaatga ggcagaaaac agcattaact tcattctcct cggctctttt tctcattcca
      421 catctttcct tttattttgc ctcttcccta cgttctccat caaggaatcg gtcatttagc
      481 gacttcttgg ctgttggttt tgacagaaga aacactggat ttctgtgaaa ggtggcaaag
      541 atgcgaagag cacatcggga attttcataa aagatcagca caaaggcctc ttgtcagcta
      601 aaacgaccca ttaaagaagt ggggagcagg gaaaatgcat tttccaaatt gaacggtggg
      661 atttacagtc ggtttaacgc cgctgacccc taacacgcta atgtttggtt cgtgtcatca
      721 gccgtccctc cccatctttt atcacctcca ttgtcgagct atctatttaa ttgaaagaag
      781 ttaattgaat gaaaatgatc aactaagctt gtattaatat tgctaatgga actttcttct
      841 tggccatgtt tggagaacga tgtcacactg gagtttggga aaggcccatt aaggcttgca
      901 cttaacccga tgggctaatt aaggaaggct tattcattcc cagagtcagg gggtaggcgt
      961 gctccaaacc catcgccttt tacagcattt tgcacttgcc attattgcgg actctggttg
     1021 cctcacaggg attggctgag attgattgtt gctctatgaa aatgacgtgc tttagaaagg
     1081 aacatttcga ttgagataat ctagctgggg tattatatgg aaatggactg gctgtaactg
     1141 ttattggcag acataacagc ccccttaaga atcttcatgc attgacatgc atgaggttat
     1201 atagcggatg tctgatggtg acgagtcata gcaacagcag cctattacgt ccagttgaat
     1261 gaggctaaac tttaaagttt gtccaaaaaa tgtgccactt t
//
LOCUS       KCVB01000490            1355 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23064pri ultra-conserved element locus
            uce-470, sequence.
ACCESSION   KCVB01000490
VERSION     KCVB01000490.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23064pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1355
                     /note="ultra-conserved element locus uce-470"
BASE COUNT          324 a          340 c          279 g          412 t
ORIGIN      
        1 cccgggcatt caaatcctgc cccgctgcgc ttttctcttc tttcctcttt attgtaattt
       61 ctcctctctt ttccttctcc tcttctcaaa cacctccact ggcattattt atttctccct
      121 gcaactccat ctgtctgacg gtctgccttg ccttttcagc cattaatcca ccatcattct
      181 gttgctgatg tggtggagga agtaaataaa cagagccaaa gccaggaatc ctaaaagatc
      241 ttgtctttgc atgaatgacg gtaattcatc actaccttgt ctctccttcg ctgtttctac
      301 tgcgtatagg aggagattta tctctaattg taatagtgag tcaggtgatg gtgaagggag
      361 ggggtgaggg ttagtgggag agagggaatt attaaatgtc accagtagtt tgatgccacc
      421 tggggccgag atgccatctt tgttttgggc ttactgtttg ctgctggtca gatggatgtg
      481 tttggtttat tggcaggtat tctctttttg acttcacctc ctggcagcgc ttttcttgca
      541 aaaagaagca ggaagagaag ccgcttgcac tcgaaagtga gagagataga tgtgatgaat
      601 aattgcaaac aataggtggg gaaatagaag aatattctca ttgtaaaaat cccccaccaa
      661 acccaggctt cctcctgctc tctttaccca accctccacc tccgcctctc ttctcccccc
      721 tattcctcac cctccacccc cattaccaat tagctctgag gccataatgg atgttgtttg
      781 ccgtgtttcc ctgtcagcgg attgaattag cgcccctgcg tgtgcgagcg tgtgtgtatg
      841 tgtgcgtgag tgatcatctt atcggctctt agtacagtaa tgaaacacag ttataaatgg
      901 ctgcaatcct gttaacaaaa cagcacctga gtgtagcccg ctagataaat cagaactggg
      961 ccgccgccac accgacccgg cggctgttgc taaggcgtgg aatcaccagg agacaattga
     1021 atcatttgct cactgtctga taaagcgtct gtctttaaca accggatcac tgctataaaa
     1081 aataacacta tttgttatca ctgtgttttt cccctcacag ggtttagttg gcgtctggtt
     1141 gaatttcttg ttttgtaatc aagctgatat tacagtaaaa cggcagcatg tttctttgtt
     1201 ttgcaacatt tggtgcttta aagacagcca ctgctttgct tctggtcttt cttcatcaca
     1261 ccatggtgat acggggttgt catgcatgca ctctttctct ctttacacac acacacacac
     1321 acacacacac acacacacac acacacacac acaca
//
LOCUS       KCVB01000491             916 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23108pri ultra-conserved element locus
            uce-1093, sequence.
ACCESSION   KCVB01000491
VERSION     KCVB01000491.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23108pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>916
                     /note="ultra-conserved element locus uce-1093"
BASE COUNT          257 a          271 c          122 g          266 t
ORIGIN      
        1 cccccccccc catcccccac ccccgccccc acccccgccc ccacccccac accaacatat
       61 acacacacat cacacgcgtc tacacacata cacattaaac aatactctaa ttacaacatt
      121 tataactccc ccatgcctcc tgcatctctc caacccccaa cccacaaatc tgtgaatctg
      181 gaattgaatt tagcccaagt tgagggctgc gctggcatga aaagactatt tgattagaac
      241 ggcagttgtt tcatttgtgg tgcagcagat tgcagagcct ctctatccgg tgtgcaatct
      301 gtattcatta ctcggtgtaa aaggatgatc catgtcctga caccaggctc atcatttcat
      361 gcagatgcac agcagctcca caaggccctt actgagccac cacaccatgt ctccccctga
      421 aacctcagca atactcttct cctttcccct gctcatcact ttctccctct ttcctggttt
      481 ctttttctct ttcctcaatg ctaacatctt atacttgttt ctctgatgac ttcttggcct
      541 gagatgaaag taaaactatg acaaatgaat gattggttgg cgggttgaaa tttatacaaa
      601 aaacacaaaa taaattgcag catgttaaac attgctatta cacaacatta aaacagcaat
      661 tttactttaa caaacactat aaattgtgca agatattttg aacacaattc atttaactgt
      721 accagtatag caggtaaaag aaatgtttaa aataaatgtg tccctataat aacacccttt
      781 ctctaaacgg ccatagatct ctgaaaagaa cagtttacag caccacactt gtccactctc
      841 tctttccttc ctttcctccc ctcttttctg cctgcaatca ctaccctttt ctttttattc
      901 catgctgtgg cttgca
//
LOCUS       KCVB01000492            1626 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23151pri ultra-conserved element locus
            uce-318, sequence.
ACCESSION   KCVB01000492
VERSION     KCVB01000492.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23151pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1626
                     /note="ultra-conserved element locus uce-318"
BASE COUNT          459 a          389 c          368 g          410 t
ORIGIN      
        1 tccattttaa aagccccacc atgccacccc atccaccatc cactcccctt tcacggtgtt
       61 ggtagtgttg gaatgttttt catcagaagg gggctattta ctcggctcca gcccctgcat
      121 gactgtgtgt ctgcgagtga aagagagaga agtaaagctc aggaaagcat ttatttatag
      181 gtatctgaaa gctgcggggc cctgaaaccc tgggatgtat tatgacgtgc ctttggaaaa
      241 gcaaaaacaa aaccccttac agccagatcc agctattttt atcacttagg ttagctccca
      301 attgtactcc ataaaagctg tgttctcacc ccaccccctt gcccacatta ccttgttagt
      361 ccaatcactt tttagtgtta cgtttttagc atgctcaaaa cccatgcagg tgtccagcga
      421 aagcattact tgccaggaat ctgtgccctg catgacaact gttaaaacaa tatgaggctt
      481 tggagtgaac aacccctgcg ccagtactac tagctgtcct gtcgtcaaaa atgtacagta
      541 cttgtttggt gaccgtgttt tagtgcagaa gccggtagtt cctaggtcca cgtctgtcgt
      601 aaaatattgt ctgaagcaga tcactacccc tggcgctaac aactccaatg atgaagtcag
      661 cacctctcaa catcttgccc cttaatggag tcttcactgc ctttgtcctt aaccatccat
      721 ttccattaat tattcagggg agaaaggaaa accgtttgag tgggtaacac tgatccagtg
      781 tactcaacca atctatgcag tgtaagtaat aaaataccac gcactcagac gacacaataa
      841 aagagatgga gacagaggag acgcttgtta aaaatgtaag tggacggaag ctacccaaag
      901 acaacaaagg gaggaggcgg cagcggctcg ggagtcaaaa gacacagtaa cgacatctta
      961 gtgtctgggt taatcagcaa aacaggccga tagcagctgg tgtttcactg tacccgcccg
     1021 gtccatggag ggcgcctgtc taagcagagg ctgagtggat gtgatgcgcc ctcagtgcag
     1081 ctgagtgatg ctaattggga ttagcgccca ttgtgctaaa ctgacacaag acattccagg
     1141 gccagccttt ccaggctcca ctcgccgcac tgtcaaactc tgccgtatgt tttctcctca
     1201 ccctcagggc tttggaaaga tgcagggcac ggaaatgcaa agccaaagaa agtgcaaccg
     1261 agaaagaaaa gacagtactt tttcaaaaac aaaaaatggg gaacaaaaag acttggatac
     1321 tctgttatag tatcagcccc taggctggaa aaggtcatgc cgcacttttg aactttcact
     1381 ctgaataagg ctagcttccc tacttgaaat atagaaaagg aaggtttgaa atccataagt
     1441 cattctgcta agatgaatcc actccagcct tgccaccaga ctcagtcagc tcttcattga
     1501 caaggggctt ttgcaccctg agtttgataa acccacccag ccattggctg tgaagagaag
     1561 acattggaga gatgattttt tcttccttta gatttttgga ggggaggggg gcttttctga
     1621 ggtatt
//
LOCUS       KCVB01000493            1501 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23233pri ultra-conserved element locus
            uce-771, sequence.
ACCESSION   KCVB01000493
VERSION     KCVB01000493.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23233pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1501
                     /note="ultra-conserved element locus uce-771"
BASE COUNT          362 a          339 c          347 g          453 t
ORIGIN      
        1 cactcttgat tgtaagtctt ttctttcaca ttttattgca tcttcatgga tgaaacatgt
       61 tgtgtgggta cgttttgact gattgattga ttaactgctg aaagaagaca ggtctctctt
      121 ctgctgttac agagggcagt gtcccaattt agcttttctg atgttattgg cccctaaatc
      181 agtgtgagcc ctacagcaat gttgctctaa gattgagtca tgaccttaag gagggtcaca
      241 ggaagattaa tatagatgat tttaggagag ttggtatctg aacttacaac aataagcgaa
      301 agaatccatt aataaccact aaatgtggaa cttaaaaatg aaagaaactt cctgtcccat
      361 cacttttctc tgtctgtttg catcttttct ccatccattt cctctcttga tgctttcatg
      421 ttatcttcct atcttttgca agtgtttatt caaagcctga tggacctgtt ggcgctcaca
      481 ctcgaagtgt taaagctcag ccttccgtct aagcgtagcc actttggcaa ataaagaaga
      541 tgaatgtgtg caaatctgaa agcaaacagc cgagattgtc agcaccgcgg gaacgcgata
      601 ggggccaagt gaatgatgat gtcgcttaac tcgctgacag atcacttagc gggcttgaga
      661 aggctgcttt cagattctcc tctcctctcc tctgctctcc tctattcctt tctcctcttt
      721 tctcctctcc tctcctctcc agggacagag atttcgtccg tagccatttt tcagccaatt
      781 tatttcttga tgtggtctaa cctggggcat ctcccgctgg ggaggcttta tgtggtgatt
      841 aatgacgggt atttagccca ggtacctggg ggagctggag cccatctgca caacactgac
      901 aggtacaaca ctgcgctcca ggaccagaca ggacaggcca ggcctgcagc agactggaga
      961 gtggcagaca ggtgtgatga gtgtcactgc gccaccgcca gccacagtta catgtagttt
     1021 attactgctg ccaagtggtt gctgccttgt ttaaccaact agaattcaaa ctttacagag
     1081 tttcaggtat tctgtctccc tacgtctccc ttttcaagta gtttcccctt catacttcca
     1141 gaacatcaca tgaaagagca agaagacctg ctggctgagg ccagatgatt tagacatcta
     1201 ttatacgctt gaatgccttg tcttcttcac tccatcatag agcatccctc agcagaacag
     1261 gcccagatgc cagaaggacc ctaatgagat taggctgcgg acaggttggt ggccagtagc
     1321 tgtcacaact actctgtctg tgtgtggaga gattaatgtg tgtgtgtgtg ttggtgcctg
     1381 tgtttgtgtg tgtgtttgca tgaaggcata cttgtgcaag cttgcatcag ggcatgtatg
     1441 tgatacacaa gtctatgtga gtgagtgtgc acaagctgcc atgttgatgg tgattaatga
     1501 g
//
LOCUS       KCVB01000494            1268 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23274pri ultra-conserved element locus
            uce-58, sequence.
ACCESSION   KCVB01000494
VERSION     KCVB01000494.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23274pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1268
                     /note="ultra-conserved element locus uce-58"
BASE COUNT          383 a          243 c          271 g          371 t
ORIGIN      
        1 gtctgtgggt atgcgaggag ttgatgcaaa agaatatcca ccaccatgtg aaagatgcta
       61 tcaatgttct gggagtaaca ctaaaggtca gtgaccctct gaggcaggga agcttccttg
      121 cattgtcaat gcatttgcag tttgagatgt gctaactcat atgtacagta cgtataatag
      181 tataagatac aactgcagtg cggcaaaaat tctgagttga gtaaatcact gttagccaca
      241 ctgtctctaa acaaagagat gcaaaaagcc tggttttcat cagtcttttt ttttacccta
      301 tagtgtctag ctctagcaaa aaatgacagt tgataacatt aaagcactgc agaacaattt
      361 caacacagca agagcagaaa aagtgtgaga ataaaacaat cctgtgatgt gactcatggg
      421 tatattttag cggagccttt tttctctacc tgagtgaaga gatgcagaga accagtggag
      481 actgtttttt gttttttgtt gttttttttc atggaaaagg gattggggcg gtggtgtaat
      541 ttccaggctg ctcgctcaca gtagatgtgg gccaatttaa gcagctacat ctcccagctt
      601 cctgctcgag cagccccccc ttctctaatg gagcagtaat cactcccctg tacaacttga
      661 cacacagact cagccagccc caaaatagag ggtataggct gggtggggga tctagcggga
      721 agagtggcac tgagagtctc ttttctaaaa gcaggtgctg aactctcagg gccccttttt
      781 gtgtttggcc atttctgcac tccccacatt gtctgccctt gttacatgtt gagaggcatt
      841 cagctggaag aaatgggaaa ttctatttct ccacaaagaa tttcaaatca ctgaagaaat
      901 ccacattttg aaagaaaatt gtcaacagct caattctttc ctccaatgag tctgaagaaa
      961 atgagccgtg gtttgaagtg gaaatgagat tatgctgatt atttgcgctg taagtaaaaa
     1021 aactgaattt aagggaaatt acatagaatt ttgtcacaat cagtgtatgc cttacctttc
     1081 ccaccatcat gtcaactctg ccttttaatt tctgtattta gttgtaataa atccagcaaa
     1141 gtaaagggta gaattaatga tgctcctgaa ttgaatgtgt tacttttgag gaagtctgaa
     1201 agaaaaaatt aaataaccct gaagttttga caatagtata caataaatgt tctgcctcaa
     1261 gaccacat
//
LOCUS       KCVB01000495            1572 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23316pri ultra-conserved element locus
            uce-1136, sequence.
ACCESSION   KCVB01000495
VERSION     KCVB01000495.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23316pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1572
                     /note="ultra-conserved element locus uce-1136"
BASE COUNT          466 a          328 c          312 g          466 t
ORIGIN      
        1 gggaactaca gaatcaggag tcaactctct gtggattcgt cactacaacc atcccctatt
       61 acaatactca cagtctttta attcattgtt tatacaaaaa atattgattg gaaccacttt
      121 agattttcta cgattaatgt tttgttataa agtattaaat tgcagactgt tcctatatta
      181 tccaaaagca acaagcattc aacacacttc caaatgatta cttccattga ctgtcacact
      241 cctggaggct gccacagtac acgcacagct gcaccaccgc gctgctgcag tattgcaagg
      301 gctcaagtgt tccaaggcca gctgtaaatt ttcttgatta actgaattgc tatgaagtca
      361 ttcagctagc tagtatatgt tcaccgcttt ccattgactt taggtgaagc atatgctttt
      421 agtcctttga agtagttacc atagagatgt tgtgtcctgg acaaacttgg tagacttaac
      481 tccttgacct tagaattgac gtgtagttca tgaagcagca aggaaaacgt tctcttttca
      541 atcttgtgct tcgccccggt tagtcataca attgaagaat ttgggagcct gaacaatgga
      601 aagaattagg cactttaaaa atgtttgcat aacgagctcc ttctcaccgc aatggttctc
      661 ttctttttaa tcacctgcac tctccagacc acctacaccc ttcttcatga tcagatacag
      721 gaccgacagg ctgatgtatg gtccgaggaa ttaatcggct aagcttgatt gccattaccg
      781 ccatgcgatt atgcaccttg attactgagc ccaccccaag gaccatgacg tgccctggtg
      841 cacattttaa aggtgcgcat aaaattatgt ggtgattacc ccacaataga tcaatccaca
      901 agcgaatgag actgtgtggc catatagacc gtctcaagta ggactgtgat tattaatagg
      961 tacttgaggt gacctgatac ccgaagcttg aaccactaaa actgcctcaa tccttcatat
     1021 aagcaaagaa atccattttc atacattctg aaagtcatta ttggattatt gttgtagagg
     1081 tagaattgtt tggagttcaa gcatggataa aagggatgtt gggctcttca gatacgactg
     1141 gggctgcact cgtctctctt aagatagact cgagatattt aatggcagca cagatgtgga
     1201 tgggcttcgc agtgaggaag gcagcatgat ccagtcttca gcctatataa tatagatctg
     1261 gtcagtgttt gcttgtgtat gaggcacaag atgttaaaaa atagattgcg cagaaaaaca
     1321 aacttttgta tgccaatcaa aggaaagaga aagcctgtgc tgacaagtag actaatgaac
     1381 atgttagtac atatcattct gcagtgacat ttatccccag aatttgggcc catttaatcc
     1441 ttcactgtca tttttaagga ttttttattt aatcctgagc caaagtgctc atttggtaca
     1501 gcagataaat agttccattg acttatccag aagcaatcct acgtcaagcg attaattgga
     1561 tgagacaatg aa
//
LOCUS       KCVB01000496            1948 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23354pri ultra-conserved element locus
            uce-1173, sequence.
ACCESSION   KCVB01000496
VERSION     KCVB01000496.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23354pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1948
                     /note="ultra-conserved element locus uce-1173"
BASE COUNT          540 a          436 c          366 g          606 t
ORIGIN      
        1 ctgcctttga tatttaagac aaaagggtac agatgtgcag gctgcacgca cacagacgca
       61 gggccaactg gaaatgggaa aggagcttta atctcactct ttgtcatgct ccaaagagtt
      121 cctcctccct gtgtattttt ctgaatctct ttggttcatc cttcgcctac ctctctgccc
      181 ttttccgctc gcaattcccg cattcataat ggcacagcgc tgtgcggcac cgaggggtgg
      241 gcaacaatta atgagtggct gttttcgatc cactgctcac attgtggttg ccacaattga
      301 ttgcatctct gaaacaaaaa ccataccagg agctgttata tttctgtgac agaagaggaa
      361 agcacaaaag aaaagacttg cgtggaaaga ttaacgattt taacactcgt agattaaaca
      421 acggacccct agcgtgagag atatggctgc gggactgatg cctttggcct ttctcgtgtg
      481 ataattttat ttaaacttac agattttttt atttgttaaa gaagctatca aagtctttac
      541 ctctttcaag aggcacgagc aagctaacag gatggttctg acatacatcc agcatcctaa
      601 tactgataca ccgtagtatc tgacatcatt gtatcattgt caattgcctg gcatagttcc
      661 accctcagga caacaatgat accatggcac aaaggcagct gtgctagtct ctctccccct
      721 cttcctatct cttctagtga ttcaacccct tcctcatctc cctcccttcc ctccctccct
      781 ccctttggta tctttctagt gcagtggccc ccgtttacca ccataaccca aattagctgg
      841 gcaggcgtta tcctctccat cgctctgcgc cgttaggaaa gggtgggggg ggtggggggg
      901 gtgggggggt ttgtatggca gaatttcctt tctgcctctg tgacagcggt atctgacagc
      961 gttttacatc tcccctgctc tcccctcact acactccact gtcagacaaa gaaattaata
     1021 cccccagtga ctgtgtttta attaagcaat ttcatgcaaa ttacacattt tagattgtca
     1081 aataatatac tgcgcaaaat tgtaggcagg ctgatctgat atgcatggag tgttattctt
     1141 ccaggcactg tgcatacatg gaaaatgaac aaaaatgcta atttctaatc tattatgcaa
     1201 acagtggaga tacttacata gatttgcacg cctttctcgg ataaatacag ctcttgaatt
     1261 ctatgtatag aacgactggg tttcttaagc agtctgccat tgttatttat cttattttgt
     1321 ctttatttta atcatatctc tttttacttt ccaagcaatg tatactaata ttctctatcc
     1381 ttccgagctg tggttgatgc tgcagggtta cataaataaa tagatattaa acaaatctgt
     1441 gaagtttatc attaaacact gtttccagat ctcattgaca atgaataatt aagagagaac
     1501 tgtctgacaa tcgaagaaca acttgacata cagtggtaaa aatcctattt gaatattctc
     1561 tgtcctaata cagtctaatg aaacatggag ccctaactaa tattagtaat ccacactact
     1621 ctccatcttt tcccattagc acattcaact tatttggaat tcattataat ccacaagcct
     1681 tttagtacac tggaatcagt aatgatgaca aaatgaatat aacattatac tttattatag
     1741 tcgacttgat ttaatctggg gttgctgcca tatgagagtc ctcaaaaata cccctcttga
     1801 aaaccatggg tgtcaaaagt cagcgtgcgc aggtaccggg tgaaaaagca atccttatta
     1861 actgaaagct tgaggagggg tcttgctgtt tctttgtgat tcaaattcat ttaatcaaag
     1921 gtttgtttct atatgctgcc acttaata
//
LOCUS       KCVB01000497            1076 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23414pri ultra-conserved element locus
            uce-590, sequence.
ACCESSION   KCVB01000497
VERSION     KCVB01000497.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23414pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1076
                     /note="ultra-conserved element locus uce-590"
BASE COUNT          259 a          223 c          237 g          357 t
ORIGIN      
        1 ggaaaaaaaa aaccctccag ggcttgttat ggagttcgtg tgaaggaaac acggatttcc
       61 acccttcttt accagttaaa ccagtatctc tggatagaac acactcaagt acttcaacag
      121 gtagagtctc tgaccgcaaa ctctttcgtg gctcctctga aaataatctc cccccttttt
      181 tctctctccg tttacctcaa ttgagtttcc agtccttttg ttcgctcttc ttatcacttg
      241 gaaaaaggtg aaccctcgtg ttatttttat tttaatcttt gctttaattg tagtttttac
      301 ccttgtggtc cctgatggct tcgcacagtg atactctggt ggtgttcttt ggggcaacag
      361 ctggtgcaaa tgggggtaaa ctgggttcgg atgagaggga aataattctc ttggtgtggc
      421 aaattgtgga tctacatgag aaaaaggtac ggacctttct gacctgttcg ttcgttgtac
      481 ggtttcttat gtatccagag tgtttgtggc atagtgtcta cttgtagtga acagtatgtt
      541 tgacatttaa atcaacatgg tgtgctctct gaaaccgtct gtagcctgct gctctcttat
      601 gctagctaac gctacatcaa ttcgccgtcc gttcgtctcg ttagctcacc tttattacag
      661 gtgtgcggcc aaaattgaac aagcaggtga ggagggtaat ttcacctaat gaagaaaaca
      721 cacctttttg gtttggcatg tcagcttctc gatgtaaaca acgctttatt gacaattaag
      781 tgctccttgc aactacattg tcaatttgtt tgtttgactt ttttattttt gtaagagttc
      841 atttcctgaa tgcgggggct atagcctgta atttgccatc aattagcatg ttttgttttg
      901 tttgtttgtt tgttttcaca atacgttctg cgccatgaaa cgctaaatac tccaacaggt
      961 cgggaagctt cagagatgtc ttgtcaagcc agacactttg gagctgacag accagtgtaa
     1021 agaggagaca ggactgactc tggacgacgt cgtcaaagct gagcctctgg ataaag
//
LOCUS       KCVB01000498            1308 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23455pri ultra-conserved element locus
            uce-902, sequence.
ACCESSION   KCVB01000498
VERSION     KCVB01000498.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23455pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1308
                     /note="ultra-conserved element locus uce-902"
BASE COUNT          435 a          271 c          251 g          351 t
ORIGIN      
        1 ttattaaagt ggagtttaag aaacatctca caaaaaatat acaaaaagaa tatgtacaaa
       61 tgatatttac actgatataa tgtaaatcaa aatatgcagc aacatttgta ttttagttag
      121 tctgtagtta ggagcaaaat gttttgcact tttcatattt tctctcagat aaaacattcc
      181 atttaagcaa tatatctgat tacacaaaac atcaacctac tgttgaaaaa tgtcacactg
      241 ttcacattca tgaaaatgcg acaataatac atcatagcag cacactgaaa agtggtcgaa
      301 agagttgcaa cagtgcattg aaacatcaat ttacagccgt tctttttgtt ttctgaggaa
      361 tttggttcgg acggattgtc ttccagtaag ccatgcacac ccactaacca caaacaaatc
      421 ctcaaacaag tatgcacacg ttaacatgtc atgaggggaa cactgccggg ggaggtttga
      481 ggtcaaccag tcaacaacac aaccacataa acaaaccata aatgaaccat ctgggtaaca
      541 cagtgaccag gtcaggtata cagtgcatag tatgctttgg catgctgaca tccagaacat
      601 aacagtggat gttacattca ggaggaaggc cacaggattt ggaattacca gtaaaaatag
      661 aacgaatgac tacatgaccc acaaagtaaa accaacgtaa tacaaaaaga atcgaaaccc
      721 tcattaagtt gtacactcag attaaggcta atacagtttg ctactgtaca tgttttaaaa
      781 aatggcattg tcagtaacac aatttcatac gcctgccaag tagcaaaact gccatttgga
      841 acatgtacac ggatactctc attcttaaaa aataatcccc cactgaaatg atatgcacat
      901 actgtattag acagatgttt gttttttcaa tgatgatgtg atgtcgtgag agcagggctg
      961 tactccctct acctaggttc acaatctgga ctgatgctgt gagatgaaaa tgagcgttgc
     1021 acaaatgcat gcacctagtt ttgaggcagt aagtcagatc aacactttga ctcaattgca
     1081 agctgccagg tgtgtcctcc tcctcttctt cctccccttc ctcttcgttt aaatgcatcc
     1141 tttagcagaa tcttttctct ggagagccat ggtgggagat gcccggactc tctgaggcag
     1201 gatggtccag gtgtgctgct cagtgagggg catggacaca cctccacaca aagaggctac
     1261 aggacatgga ggaaaggatg catcacttaa catttgatgg tcttatca
//
LOCUS       KCVB01000499            1534 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23497pri ultra-conserved element locus
            uce-141, sequence.
ACCESSION   KCVB01000499
VERSION     KCVB01000499.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23497pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1534
                     /note="ultra-conserved element locus uce-141"
BASE COUNT          463 a          304 c          307 g          460 t
ORIGIN      
        1 tctctgcctt gctattcaaa caagagttag taaatgttag gttattatac ataaatactc
       61 acattatgat acctgtcttt taatctcatt ttatttgttg aaatatgaat tgggttaatg
      121 ctaattatta tttaaagtag ttttgatgca atacacacaa atagcagagt tggtaagaat
      181 gttaaaaatg tttgattaca attgatgcat ttctttttaa attgttcagt taaatctgat
      241 gtcggatgtt atctttatgc tgactttaat aaagctggtt gaattaattt tctttgcttt
      301 ttatggctgt actcaatttt ttagtccttc agacgcttta tattattgag ttttcacaac
      361 tgaaaatact aaagagttca ccaaataaac aagaaattat tggaaaatgt tgacctgatt
      421 tttttagtgt gagattaaag taagagtttt tacagcgtat aagcactatg tacagtatat
      481 atagagatct tggatacaac ctctactgta tgtgtgtgca gtgcattcag tctaagctca
      541 aattgttgcc gagtgtgtgc gtgtgggctc gggggactat gtctatgggg agaagcagag
      601 caggagaagt tggagagagc agcagccaca cagaacatgg acagagcgtt tacctggccc
      661 tgtgctctgt gggtacgcca agtagccgcc tcttccacgg gcccccctac ctgagccacg
      721 cgctgctgct actgccgctg ccgccaccgg tccgtacgct gtcgctggga agcctgcagg
      781 cacatacaca cacacataca cactacatca cttcctgcat atggatgagt acaccccaga
      841 gctacaggag aatgttgata ccagcactga actatgggat aaaaccatag ctgtcagggg
      901 ctcatgtcag ggtgacatct atcagcagac tcttaccgaa cccactgtga tattcaaaag
      961 cctgcagatc cactgattca cacacagaaa cagaaagatg gccatgagca actcagcaaa
     1021 tataagattt aaataaaaac aactgacctg aagggcagct ttgacatgct tgtcatgatc
     1081 atcgtgaggc aaatatcaaa ttatataaaa gccaacaaac aaaaacagca gtttatgatt
     1141 ttgtgatttc aaactttata aacactaact tagattagag caaattgaac aaattgcact
     1201 ttaaatgaat ttgcttcaat acaaaatgtc tttccgactg aaaaggtgca agtttttact
     1261 catctacaaa actgtgagta aaccgacttc acaatccacc aagtccctga ttatattagt
     1321 gaaagcattt ggctcttggg ggtgtttatg tcacatgctt tggatgaaag attacagcct
     1381 cttctgagct gcatagacat gggcataata gccctgcctc tggggactac ccctgcattt
     1441 tgtccgggag tgctcccaat tactgtgagt ttctatggaa tcagaatcat ccactgtaat
     1501 gcaagaggca atgcattcca ccgttttttt ttgt
//
LOCUS       KCVB01000500            1655 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23564pri ultra-conserved element locus
            uce-1237, sequence.
ACCESSION   KCVB01000500
VERSION     KCVB01000500.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23564pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1655
                     /note="ultra-conserved element locus uce-1237"
BASE COUNT          492 a          322 c          331 g          510 t
ORIGIN      
        1 acgctacttt taatgaaaat caaacgaaca gaacaaatga agtgtactga cagactgtac
       61 gggtatgtga tgtggttgtc aggctgttgt cagatcagtt gtgacacaaa cggatgaaca
      121 gggctgacag taaaccttca gctgcgcctc cctgcaggaa tcaatacact tcacttctgc
      181 ggaatctgaa acttgttttt aaacagctat atcgtaaatt aaaatagact tctaatgtaa
      241 tctgttgcca aagtgttcga tagccgaacc aaaaataaat gcacctaaaa tttgttatgg
      301 tacatgaatt gactgagcat tttttgctgt aggtctttag taatgaaatt tgaaaagtga
      361 cgtttcatcc gtttagtgaa tcctggcggt ctaaaagcct agcactcagt catttcttct
      421 tttagaaatg gtgaaaattg ccacatataa caatgctgct ggggtttgat tttattggtc
      481 agagtgcgtt cagacttgta aatgggatta tgccatccca atatagtgct tcaacaacta
      541 gcttgtttat ttttatcctc ttattaggag agttggatcc ttaaagggtg ccaaaattat
      601 cctgcaaatc gctgacaggg aaaaatgaaa ggtctctaac ggttattata agcaatctgt
      661 ctgcattgaa gcacaatgtg actgctgcgc ttgcgataca acgaggtgac ctcagtgaga
      721 tggggaggag agacacgttg aaagtcagac acaccgtctg gatgggacca ccaggaatat
      781 acacacacct cacacacccc gtttacacca catccacgct caagaggaca cttagcatgg
      841 actgtagaag cattaaagta actgctttac acaatatatt acttgaactt ctgtgcagta
      901 cactgtatga atactttaca tttgaattta acagtatgtt gaacaatcct tacatcaaaa
      961 tcttttactt tactatattt ctgtaacata aattaaataa tgaaaccttg tctaactttc
     1021 tttgttgcct ataacagcct gtggactgat ttatttgtaa aggatttggg atgaattttc
     1081 caggaaaatt taaaggatgt gatgtacatg gtgcccccga gtgccttgtc tgcctttttt
     1141 tcacttccgt acagcgctaa cagtgtgcaa cactagctaa cgttagcata tcgagtttgc
     1201 taatgtcaaa gtctaaagtc aaagctagaa taattatttt tctgcttcca taaaccaaaa
     1261 acgtttgaat acttttagaa tatttttata ttcaatcaca caagagtttg ttgagtaatc
     1321 taaaggaaaa tgatgcaggt aattcactta tccctccact gtctgactat cgacgtcatg
     1381 aagatgctct ttgtcaaatt tcttcatcat agatggctgc aggctgctga aggagttggt
     1441 atttggatat atctcatgag ttagcagagc aaaatcactc tgctggttga ctgcttggac
     1501 cccagggagc aaattaacag tatcaagctc ttagcttccc tgtaaccttt caccatcctg
     1561 actgacattt gtgttttcct ttgccttggg cctggacgta cggcggcctg tgagaggatg
     1621 cacagagatg ctgcatatgt tagaacaact tcttg
//
LOCUS       KCVB01000501            1542 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23601pri ultra-conserved element locus
            uce-1272, sequence.
ACCESSION   KCVB01000501
VERSION     KCVB01000501.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23601pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1542
                     /note="ultra-conserved element locus uce-1272"
BASE COUNT          374 a          364 c          319 g          485 t
ORIGIN      
        1 ttttcaaggc caacactgat aattctgtgt tggacttgca actacaaaag ataaagtatt
       61 tttaaatgtg actgtctttc caagaaatag agaatttgta aataaagttc caggttagga
      121 gtcagtaaac caggctttta agtacttttg tgcacatttt acagtcattg tatagttttt
      181 aagattgtta tatattagaa tgaataagtc acacagttat gaagaaacac agtcaaatgt
      241 atcaactaca tttagggaaa taaatgcata tatttcagta tctgatttta atatacagga
      301 gagcatcaga ttttatgact ttgttttcca agttacttca gaggttaacc tgaacatctt
      361 gtattcaaag cctcactctt tttcctctgc tttggttttc ctaattatta tttgattgct
      421 cattaggcgc tgtgttcacc gtgcatgcat ctgttcatga aacctgattt ctcatatgtg
      481 agagaaacaa ccatctccct ttaattacac cgcctcgcca acatttgtta acgctccttg
      541 gaggatgcat gtggacgtcg gcaaataaaa tgcagcgcag tttacaggat gatgtgtctc
      601 gatgtacagt aaacttaata ccagacatac tgtatgtaga atactttgaa gtaagaaacg
      661 ttcttttcat taaactatcc ccgaccctct catttctagc agctgtccct gcggatacac
      721 accctgcttc ccttctctta cggggtatga tgtgtggatg aaggctcttt cccgtgccat
      781 aatgactgta caccaagatt ttcaagggcc cgctcatgac aatcccattt tctgtgtaat
      841 ttcatggttg tcttcttgaa tgaagatatt ggctgctctg aagcacgagg tcagagtctc
      901 cctgctccgt tttctggatt gttccggagt atgtttccct tcacttgatg aaaagtaatt
      961 catcatttac acccctgaat tctgccactt ttttcctttt ttttccccct cccctcgcca
     1021 tgctaccgtc tctcttttca gtaacaactt tcttcacctt ccctctcctc cccttcgctc
     1081 tcagattccc ccgtggtacc tatggtggtg ttgagagata gataggttca atacgacggc
     1141 gggtcagcca tcctcgagaa caggttatcc ctcccaggcc tctgcgctgc cctctacccg
     1201 tgatagagtt tgtcacctcg ctacctctgg aagtcaaatc ctgtcaactc aggcatcctt
     1261 cagtatcccc gaagtggctt tggcacccag cggggcatgt gagcttaaaa ttgatttttc
     1321 tccccccttc tgtgtttttt ctcctcctcg agatctgcca ggttccctta agggaggcag
     1381 ttgacagcca gtggaggccc tcagccaatc acgaggcagc ggcggtgtaa aagacaccta
     1441 gggaaacttt ccctgcacct cctggagccg gaggggggca aaggtttctg acatgttcag
     1501 gaggaggtgg tggaggattg gggagggggg gatgctgttg ct
//
LOCUS       KCVB01000502            1410 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23646pri ultra-conserved element locus
            uce-691, sequence.
ACCESSION   KCVB01000502
VERSION     KCVB01000502.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23646pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1410
                     /note="ultra-conserved element locus uce-691"
BASE COUNT          448 a          272 c          284 g          406 t
ORIGIN      
        1 aaagtttgaa gttaataact tctgtgatat ttctgtttcc agtaattgaa aattgcaagt
       61 aagcactgtc tgagaaatat agacaatatc acccttcatg caaatatctg ctaccactgt
      121 ctcaagggct gccccaaaga ggtccatgag gacatgtagg aaacactagg ggaggatgcc
      181 ccttcttaca gcatgctgaa gaagtaggat gctgaattca aatgcgacag aaagagatga
      241 ctgcacaaag gtagtcagaa agtgctgact ttttaaaatt ttaaattacc tgaaaacaga
      301 aattccaaaa aagttagtaa ctttaaactt tctgcataat tgcttaaaga tctgaactgc
      361 acaagcatgt aaagaaagct atataattta tctcctttca ccttaggcca cgatcttatc
      421 aatcatccct cgtagatcat tataattaca ttaggtacat ggagttcttg ttctctttga
      481 tccacaacac tataatcaga caattaacca tgttcaacat tgttgtttat tgtgtgctga
      541 aaatattcct tcaactattc agctaatgca gacataaggg tgctcgagta ttgctccaac
      601 tgcaggaggc aaaaagtcac ctcaggtcga tggcactcag cctgtaaaca gggcttcccg
      661 taaaatcaat gatcttcagt aggagctatc agtctgtgaa acagaaatag ccgtggctgt
      721 gttgagtctg aggcgaggaa ggacagtact gtgtgtttac atgagtgtgt atgtgagtaa
      781 aggggggctg gtcactggca tgtctgggag tatttctcac agatagcaga actgaggcat
      841 gcagcttctg ccatgttaca gctagtaaaa acctggaagg attaggtgct atagagacag
      901 gctacagctc acaggtttca tcaatgttgt tttgcatgtg tactttatgc atcaaactag
      961 tactggaaca gatttatcac ttatagctaa aataatcctt gctgattaca atatgagcaa
     1021 actcaaatac ccaagaggca atgcgcttga ttgctttagc tttacagcac tcaattttag
     1081 taatcatttt gcactagtaa tactggccag aaaaggcaaa attgatttgt ttgtagtatc
     1141 aattgcctcc tttgttaata gacttgaaat agaaccttga aaatgatttt accctttatg
     1201 caacaccatc aaattctgct aacccattcc atgttgcaga acggaagaaa agtaatttca
     1261 gacaaagcag cagtgatata acggtgcaca actgtatgtt actcatacac agggcagtct
     1321 gattaaatga tctgattgga gctcagctaa cttcccaaac cctccagagg ggatttacac
     1381 acggcactag agtgtttcct cagttttgga
//
LOCUS       KCVB01000503            1615 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23691pri ultra-conserved element locus
            uce-205, sequence.
ACCESSION   KCVB01000503
VERSION     KCVB01000503.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23691pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1615
                     /note="ultra-conserved element locus uce-205"
BASE COUNT          456 a          329 c          377 g          453 t
ORIGIN      
        1 cggatgcaga catccataat gcaatcttcc taattttgat ccctcgcaat ctggttttta
       61 aaagggaggg gaaaaaaagg gacatatttc tgaaggcttg ctccataatc attttagaca
      121 gatagccttt cccatcgcag atgagcaaat atttgtaaac cagctatcta atggaaaatt
      181 agagtgaaat tagagtgaaa ttcatgacag tgaaatccga ttatctgtaa ttctattatg
      241 ccatcttagc ccacacagca tatttttgta gagttttttc cataaatacc tatgccaggg
      301 gtcctgctaa tcaaataaaa ttgaaattca tcttcccatt aaattcaatt agctgcaatt
      361 tctaaagcct aacaaagatt ttaattacta aattataaaa ttctccctag ccaaattggg
      421 gagagaaaaa gtgcataccc tgcttctgaa aaacaaaccc atgaaaggaa attgttgatc
      481 aaaaaagata tccaggggaa aataacatta gggagagcac tggggccttt attcagcgag
      541 tctgggtggg gagtgacgtg tgccaagcgc taggctcctg ggccggagga aatgaactta
      601 gctgaggttc tctctgtctc taaatgtctg ccctcagagt tattatgctg taattgacta
      661 attgacacct taatttcatt tatggggctc ctgtgttcat aaagcaacct ctgggtgggg
      721 cagtggtctt gagcggaagc aaggaagtca acatccagga caacaggtag ggccttaggc
      781 cttcagttct gtcctcggca gatgacgatt atgcatttga taatgaaatg gtcaggaaga
      841 acatactgac ttagcagagg cttaaggagc atcttactca tatgtttaaa tccctctgat
      901 gaaggctgat gggtaataat catgcatgtc cggtcttaca atctggcact gcagacacaa
      961 atgacctcct tcagggtcaa aggagtttat tttcggcaag tgaggttctt gcactatcca
     1021 acggggattc aagcggcgtg tttggtttgc tttcacagca ctggtgaccg tgggtgaatc
     1081 ctgtattgtt gtatgccggc tctgttccat tcaagagtgc aactgttgct ggtaacgtta
     1141 agtggagagc acactgctaa taggacagct ctggggtctc aggtcagcca ccaggtccat
     1201 ttcccagcca ccccctgctc ctcaccagtg ggccactaaa ataacagctc agcaccctct
     1261 gcagactaca aagctccatc tgaaaaggca cctgctgctg ttattcactt tagccccttt
     1321 ggcagctcac tctgtctgtt taaaggacac gtagaagaga aagactgact gggaaatgat
     1381 gaattattta agtatgatgt ctaattgttt caaggcagac atcttgtttc tggaaggtaa
     1441 aggcttgggg gtgggggtgg gtgtacatgc ggggggagga atctttgtga agaggtctgt
     1501 ctactgtgta atgcagccag tctcgcactt gaagtggctg ggtaaatatg accgaactga
     1561 aactttaatc tgctttttcc acacccaagc tggctgggta ttaaagataa tcttt
//
LOCUS       KCVB01000504            1585 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23730pri ultra-conserved element locus
            uce-710, sequence.
ACCESSION   KCVB01000504
VERSION     KCVB01000504.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23730pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1585
                     /note="ultra-conserved element locus uce-710"
BASE COUNT          488 a          305 c          311 g          481 t
ORIGIN      
        1 atgctgtaga gacacgatga tgccacagag aactgtttgt gctttgatat ctaaggaagt
       61 tactgtgatt taatcttgat gtgtttatca cttaaggatg ttgcctacct tcccaaagtt
      121 cacactttca ctctggagtt cttttgtttt tttaagccgt aacatgaaga atctcttaaa
      181 gttttttacg tattaaaatc aaaactttat ggcaattcac tccctgcctc tatcagaata
      241 tatggcaacc tcataggttt tggttggata atagttccct tgtatttgaa ctctactacg
      301 cttgtgttta ttttttgaag acaaaatggc acatcgctat aaataacttg gtattttaca
      361 cagatattgt cttaagagcc gctgccatag aatttctgct ggtataaaaa cactttatca
      421 aaggaagcat atgtttgtgc gtgtgtgtgt gcaagactac aagcattgtg tttctttaca
      481 aagtcccctc tgccatgtga gtaactggca ggcaggtctg attatgcaag aataaaagaa
      541 aaaatgatgc accaaactgt gagtaaacta tcactgattt aagcaggtca gtttttgtgt
      601 tttatttaaa aagaaaaaaa aaaggctgtt taatgtaaaa ctcccaccat atttgtaaat
      661 ttcatgatgt gttgccgtaa gagacaaagg tcacactttg atgactttgc ttgtggttcc
      721 ctgagtgttt tcctgaatct caagtatcca ataaactaca catgtagtgg atacgtacac
      781 agccgaccct gccaggaaaa ccagcccaaa tccttacagc ataatacttt aaataaatca
      841 cgccacagga aagaaacagc acaatcattg tttatggcag aactacagtg cttatcttca
      901 aagcaacaga tccttttgaa tttgtatcag gtctcagacg agcagaagag agaaaaagag
      961 cgagctaaca ggcacaaaca tggactctgt gagtgtgtgt ctgtgtgaga gaataaagaa
     1021 gtcaaatcga gcattttctc caccaattac acttaagaaa agagacattc actttggcta
     1081 aaaagatttg ttagtctgtt ttagggaggc attttatctc tgcttcagct ccaaataatc
     1141 taatgtctga gtctaaccag ttcacactat ttaattaaac agccatgcgt tgctgctgga
     1201 gctgagcagg ccagattgtt ttggttatct gctgccttga atgagccgtt taggacagac
     1261 acatatcacg gtgtctcaca ggtctctcag tctagttcca gttaataatt agggctaatt
     1321 acagctaaag gctggcctct ttctcctctg tctgctgagg acagacgtgt ggctctgttg
     1381 ctggatcaag gtcacactta ctcaccaact gcaggggaac aatttgtgtc caaaaacatt
     1441 gatgtaagaa atggcccgct gtggcaataa gccactaaca tccaaagctg tgtggacaat
     1501 aaaagacttg gaagacttat tcaattcctc agataagagt cttatcagta gcagaataca
     1561 attcctaatt tacaaagaat agttg
//
LOCUS       KCVB01000505            1288 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23772pri ultra-conserved element locus
            uce-755, sequence.
ACCESSION   KCVB01000505
VERSION     KCVB01000505.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23772pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1288
                     /note="ultra-conserved element locus uce-755"
BASE COUNT          377 a          271 c          301 g          339 t
ORIGIN      
        1 ttcatttatc atctgcaaaa acaagaggca aaaacaaaac tgcaaactat cacaaatctt
       61 caacggtgct ggagtcagtc tcaggagaag acagtagact ttttactatt aattttaagt
      121 tcctatgcaa gcatttataa aatgaataca gtgaaattaa aaccatgccc tttcatttgg
      181 aaatgagtta gcataatagt tgtaattagt gagatgaaaa gacaatatta tttgaaaaag
      241 ctaaaagcaa tctcgcctgg tgagttagtg ccactcaaac tcaactacag ggatatcagt
      301 taagggcagg agatatcttt tgcttttcat tctgatccaa gatctgccag gttaggcagg
      361 agtcaggtgt cagcccatcc ctacactgtg gagatttagg acagagagtc aggctaatct
      421 ctctgctgag ggttagttag tacacagtca tcagtggcgt ctcagttttt gcagggattg
      481 tttgtgtgag gtagagatac ctccacctcc gaggcctctt cccctgttgt gtttctctgt
      541 gatcttgcag tcatgtatgt gattggattt tggcttaaca aaaacctgaa attacacacc
      601 tattgtgttt cagttaagcc cgctttcact gcgatagggg caggctctgc acagctctct
      661 tgactgccga cttttgggat cccgcccctt gtcccttcca ggttgttgat aaaaaggata
      721 acaggattag gtcagggttt ctgggaagca tcgattgcca tatttggcgg gctggtaaat
      781 aaaggagttg cgacatgggt caccggctac gtggtgagac gggctcggcc tttaccctgt
      841 gtgaggaaag atggcacctt ggcagagcaa ggtgacagga cttgggagac gagggataag
      901 agctactcac catcttctaa accacactca gggaccctga cactgcacca tctgtgacac
      961 tacccccagt cccctcaggc tatgcttata ctcatcacag agatgctgat aacacagggt
     1021 gtgactaaca cgccatacaa atctccacat cccaggcaca gagaggaaca gaaagaaggg
     1081 gacacacttg cacagacata taaacacact tacacataga ctacattcac tattataacc
     1141 agtcttcaag aggcaggttg ctctcagatt tcaggggagt gtggatattg gtcttagatg
     1201 agtgggtgga cccagacggg gtaatgacag ggtaagaact atgtatgttg agtaatatgt
     1261 aattaaatca gaaactgaaa atactaca
//
LOCUS       KCVB01000506            1574 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23809pri ultra-conserved element locus
            uce-411, sequence.
ACCESSION   KCVB01000506
VERSION     KCVB01000506.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23809pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1574
                     /note="ultra-conserved element locus uce-411"
BASE COUNT          498 a          355 c          269 g          452 t
ORIGIN      
        1 atcatttatc accaaaagag cctgtcattg tagtgcagct tttgcacctc caactctcct
       61 gcctgaactc tcttaagaca actgagtggg ggaaaaggaa ccccaactcc aaagtaaaaa
      121 cctgccagac aacaagccaa caacagcagt taaaacaata cctaaaatac caataaatgg
      181 atacaagccc tgtatggttt tcgggagagc tggcaataga aaattcaacc cggccaactg
      241 gtggcttgaa gagaggaagc ccactcttaa agccgtgatc cgaccatcca ctataccacc
      301 gctgtgagcc ttttaaccac agcagaaaaa aatgactatt gatcttcaaa tagcgataat
      361 tgaaaagatc aaaaagatta aacaaaaaaa tcgagaatgt gctgacttac catgttatgc
      421 tccttttttg ttgtaggttt ttgtttaaat aaaaaaaaat atttgctcaa agatgcagca
      481 ttggaggtgt caaatatttg tagccgtctc ttgatcgtat tacttccaca gtcctggttt
      541 ccaatgcgtc tgaacttttt ccctttctct tttattttgt ttgtggtacc tagccttttt
      601 ttcccctgct taagacttta aaagccttca gctcagtaaa gcagcacaaa ttatcttgcc
      661 accttgctca gctctgatgc tttggccgct aactttggaa ggccctttac tactgcatca
      721 aaattagcat tgtcaaagca gttaatgaat attaatattg tatttgtcat tggagctggc
      781 ccattgctga actccaagtc atccatcagg gacaagatta agcacacaat tatttctgac
      841 tgacagggac caaatctgga agattttttt tttcctccaa caatttaaag aaaaagacac
      901 agcatcatct taaggtacct cccatgagac gagacaagat taatacactt ttaatgaagc
      961 aagatttcag ggtgcatgaa cagcaggaac ctcaagaaaa aaaggagagg aatatagaaa
     1021 tattttgagt cataatttta accatttttt taaggattga aatcataaat tacaaataat
     1081 tttgaagatg gcacactaaa tttaaattca aagaccctct ttttttttct tttgatgaac
     1141 ggatttattc cccttgattt tctttttaca agaggatccg gatctgcttt ttcaccttcg
     1201 gtgcaacgtt aagagaaata agatgtacaa gcgctgcact gtgaacaatg ctggcgctaa
     1261 atttaacatg aaacattcat taaaaccttg gacctccagc actttcggca gagccggcgg
     1321 agcaccgacc gctgaggacc acacaggcag cctccatcag caccacccgc tgcatccagg
     1381 agcattctcc ctgctccgcc acccgcttcc cacggaaaca gcttcacact aggaaccacg
     1441 gctctgtcaa atacggctct ctgcacaatt ttccagacct ccatcaaatg tcacaatgca
     1501 caatttttta gatcttttct aaataattat gtcattccaa atttctcatg aaaattattt
     1561 atgttatgat taaa
//
LOCUS       KCVB01000507             941 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23909pri ultra-conserved element locus
            uce-81, sequence.
ACCESSION   KCVB01000507
VERSION     KCVB01000507.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 941)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23909pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>941
                     /note="ultra-conserved element locus uce-81"
BASE COUNT          225 a          274 c          205 g          237 t
ORIGIN      
        1 cccccacaca cacacacaca cacattcaca cacattcaca cattcgcaca cacacacaca
       61 cactacactc acatgacctg cattgctctc tttccagcta taggcaaact gcagcagtgg
      121 ctattaatgt ttcacaacat agtgtatctg tgtcttggtc tccatttttt aagggcacac
      181 tgccagaggg aaagcgtggg aggagtttgg tggagggttg ggtgttcaga cacagacaat
      241 atttccccca tctctcgtgc ctcaatgctt cactgctcgc cagactcaat cacatgtgtg
      301 gtgtgctttc atgaattaat aacgggcact ttggaggcct ggcaggaaca tggtgaccac
      361 acgctgatgg aagtttaatg agcagcacgg ggaccgcgcc gtcatggcat tggcgggtct
      421 ctctgcgcac taggccatgt ccgcagccct ccccacagag ccacagtgtt tgggctctca
      481 ttacctagat aaatatatct gccctctgga tcagcccaca ggccctgctt cctcctcctc
      541 ctcctccccc aagagatgct atcacagccc tgattacact gtcatgccct tgggacaatg
      601 gctgggctgg aatcacaaga gcacaaacat cagtctcaat tcactcctct gctaaatatc
      661 aggctttcgc aaagtagtag ctcggccaca gcatgacata cgttcaggat tgtcgagaca
      721 gagatcactg agagctttga ctggagtaag gttcaatggt gtttccaaaa ggcaattatc
      781 tcagtcggat cacatttgga ttcaggcagc agcacagggt aattacggag gtcagtagct
      841 aagagtgtat cccctccctc cccctctttc ccaatgtcta tctctctctc cttcttcctt
      901 gctatccagg ctctctgggg actgcccact gatgagtctc t
//
LOCUS       KCVB01000508            1326 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23950pri ultra-conserved element locus
            uce-221, sequence.
ACCESSION   KCVB01000508
VERSION     KCVB01000508.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23950pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1326
                     /note="ultra-conserved element locus uce-221"
BASE COUNT          389 a          288 c          254 g          395 t
ORIGIN      
        1 cattcatagc tcagccactc gcagagcaag agggggaata catactggtt aattagaact
       61 cccattaaaa caaatactgt ttcaatttgg caagtataaa aaaagatgta agaggctttc
      121 agaaaaaaga gacctgctgt tggcagctgc caagaaaatc cagggactgt aaatcatatc
      181 cactggctat actgtttctg cacagattat tagtgtttgc tgttgttttg caacactttt
      241 gaactctgag gaaagattct gcgtctgtga caccagaaat gtacctctgc acacatcgtg
      301 ccgcgtaccc tcgcgttgcg ttggccttcg tcctctgtaa gggaatgtat cccggtttgt
      361 ttcggcagct tgcagagact tatagctgca tggaccgtag cttttatgaa gtatgacata
      421 aaaaataaca ttgtttctca gtgtaaactc aaagctttat gacactttac agaaccgaag
      481 atttccactc atagaggaca tcatggtcac aactgcagct tgggcctcgt ttgcttgcag
      541 ctaaacagca ctgcagctca ctagctgtct gtgtacctga gccatttgtt gctcacttca
      601 tcttttaggt ctccagagca cccgagtgaa gccattttct ttctcattcc aaaatatttt
      661 tggtctgagc tttatgtatt cataatctat ttttcaccta ccaaccttcc ttccctccat
      721 ttggcttttt gtcatcccat gatgccttgc caatagtgct ttgctctctc cctctctctc
      781 cccaccttcc tctcttgctc caactctctc ccctggagag gcagctcttc tccctacgcc
      841 cagatgaaag gctatggcgg acaataaaaa tgctatttaa atgcaagggt gtttgtgtgc
      901 gggtgagaga tatttcctgc tgaaagtgag ctgctgcatc ctgataaata gtaaatataa
      961 gggaatgtga tttacattta tcttgcagag gcagccaata tgaatttcag taaatcctag
     1021 aagatgggga tttaaggagg aaaatgactg agactgattc acatgctgtg tgtcccacgc
     1081 agttgaaatg cctataaggt ggagagtggt actgccttta ttaaagtata ggcagagaca
     1141 tcgatcctat ctaaaatacc tattacacac gaacatgcac tgtgtcacat gttcatctgc
     1201 ggagatttcc agcctcacac acacacaatt tgaaggtgac ttgtattgaa ataacaataa
     1261 taataataat aataataata acaataataa taataataat aataataaca ataataataa
     1321 taataa
//
LOCUS       KCVB01000509            1333 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 23995pri ultra-conserved element locus
            uce-1213, sequence.
ACCESSION   KCVB01000509
VERSION     KCVB01000509.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="23995pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1333
                     /note="ultra-conserved element locus uce-1213"
BASE COUNT          368 a          283 c          304 g          378 t
ORIGIN      
        1 tccagttcct cagagcgggg agtccttttc agttgaagac atcgaggcat ttgtctgaga
       61 agggttaaag cagcacaaac tgctaactgc ttaaagaaat ttaacggaac agtgaatgga
      121 gagaaattaa ctgttttgtg aagcctgtca ataagatacc aacaatctaa ccattgcaag
      181 gtggttctgt ttgctactgt aactcaattt aaggtataag ttaaacttag ttctacccaa
      241 acttaaataa aaatgattgt ttggcaggat taactttact tgctttaatt acagaaacat
      301 ttacgcagtt atctacagcg atatggttat ttccttgtat tacggttaaa ctcagcctca
      361 gctgccgtaa atgctatatt ttttaattct tgatccacac ttttgttgtc cattttgaac
      421 aaaagaaacg aaaacactga ctaattccct gtaaatgatt gtatttgaac actattctta
      481 ccctcttctt tcagggcatg ctacggtgtt ctgaggttca tcatggagag cggcgccaag
      541 ggttgtgagg ttgtggtgtc tggcaagctg aggggtcaga gggccaagtc aatgaagttt
      601 gtggacggcc tgatgatcca cagcggtgac cccgtgaact actacgtcga cacagcagtc
      661 cgccacgtcc tgctgaggca gggtgagtac agttctacca gggacgcacc cgcaacacag
      721 aagataacag ctatgagttt tcttctgggt cttattggaa atattttcac acagttgcca
      781 ctataacaaa accaactgat actttacacc ccacaagtat ttgaaggagg atttgttttt
      841 attttggctc cgtacctcac acctttttgt ttttattgct gctaatacat gttggttgat
      901 gtgtttgttt gtcgccctca ggtgtgctgg gcatcaaggt gaagatcatg ctgccctggg
      961 accccagcgg taagattggc cccaagaagc ccctgcctga ccacgtcagc attgtggagc
     1021 ccaaggagga gcacctgccc accacaccca tttctgagca gaagggggcc aagccagagg
     1081 tgcccgtcat gccccaggga acacctgtac ccactgcata agaggtaaga gagcagggtc
     1141 cagtcagcgg caggctgagt ttgaaatgtg aaatggaagt taccctcttt gttacatatt
     1201 ttggtgtaat attccttcct taaacagaca gtgttaaagg ggttagtgta ataatcagtc
     1261 tgtcagcata gtgagcacac aacagaacca atttctttta cataatggct caatataata
     1321 ggaggatgat tat
//
LOCUS       KCVB01000510            1649 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24042pri ultra-conserved element locus
            uce-1256, sequence.
ACCESSION   KCVB01000510
VERSION     KCVB01000510.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24042pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1649
                     /note="ultra-conserved element locus uce-1256"
BASE COUNT          533 a          286 c          310 g          520 t
ORIGIN      
        1 gatgcaccat acaccgtgca ctatacgcat aatggcataa cacaagcttg tttataaatc
       61 tgtgaactga tccaagcttt atccagctgg agcatgaact ctcaaacttt ctctcaacct
      121 cagacagtac aggaaagaag gataaaggca gagagggagg aaatgctgca ctgtatcata
      181 gggtagtgtc aagtctatgt acaacaggct cacatgtaga gtttacacgt gttcaactat
      241 tgtggaacat catgaactat tgtacctttg ttcctgcctt tcttgtcaaa tcaatcagtt
      301 tgtgtaagcc agtaaactca atattagatt gaaccctatt attattatta aaaaattagg
      361 actgagttat gttagataca atcagtaaaa aaaaatttag cttttatgcg ttattcaatg
      421 tatggcaaaa aactgtaagt agacttaatg atttgcagga aacttgtttt taattttgca
      481 gaaataaaag tgatgcttaa aaattgtatt cagtcagcac aacagtgagg ttttttttaa
      541 ccatcgctgc ccgattctca ttagataaga taagattttt aaataaccgg tggatctttt
      601 ggacacatgt aggaaacttc aagtcacagc tttcttaatt tttcacgttt tgcacagttt
      661 ttgtctagat cgcgtttttg ccgtcagatg tatttgcatt aactgagttt aggagaacat
      721 atcagcactt atcatgtatt ctcatttggt ggaatgagtg tggtgcgcat cagtaagagt
      781 gttgctgatc acagcctgca tttgccttaa ttttctgtgt attcctgttg tcctgttgtg
      841 tcagatcagc tttaagataa gcagtatgcc atttcaaaat tatcattcag ggaaagaggt
      901 agcttgtagt gtaaaatcaa tagctgctta aaattcattg ccaggcaatc catttgccct
      961 ttatgttgct aacctttcac ttaaatatgc accatattaa gatttcatta tatacaatga
     1021 atgtcacaac agtttgccca ctatttctct tgtttgcatg ggttaactgc aaatcattgt
     1081 gcttgtaatg tctgaaaaat gactttcatt aacattatgc agaaagaaag gaaaatatat
     1141 gagcctgaga agcctaatag gtattaaaag gcatcacagg atatgtatgg tgtttgagat
     1201 acaaaggcct tttagcacac tgggtgggtt gcatgtgtct gaccccagtt aaggaaacac
     1261 agagtaacac aaaactacag ccagtattct ggtgctgggc actacaccac aacatttgtt
     1321 gatataattc aacacaaaag ccatatcatc actgcacata ataatcatat ctggaaacct
     1381 gtcttgaaag gaaagagcat actgaaaatg tcacttattt cttttggcaa agtgcaatta
     1441 aatactgaga gtttaacacc gtccttgcaa aaacagtcgc aatttagtag cgtgtcaagt
     1501 ttttatagtg actgaatact tggtcaacta agtaacctaa acaattagca gagttttcct
     1561 aaaagtacac agaagtacag tacatttaga taagattgtg gtaaacaaac gactttgtca
     1621 aaatgtaatc aggcaggatg atgtattgc
//
LOCUS       KCVB01000511            1361 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24121pri ultra-conserved element locus
            uce-165, sequence.
ACCESSION   KCVB01000511
VERSION     KCVB01000511.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24121pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1361
                     /note="ultra-conserved element locus uce-165"
BASE COUNT          413 a          244 c          278 g          426 t
ORIGIN      
        1 tgttttaact catttctagg actgtgatat tttcatagct caatttgtaa catgaaccac
       61 aataaaaact tttaccatca tttattgcat tggtatatta aagctcctgc tgatattttg
      121 tattacgtgt ttacggcaag gaacatttgt catgtccggc tgagtaaatt cagtcacata
      181 ttgagaaacg atttaaaata atttattttt tgtaatattt taacatccag gcgtaggtct
      241 agatttttag aaataccttc agaacagcag ggagtgtttt taaataatca tctggatgtt
      301 ttaaaggctc tccaggtact tttctttttt tcttcagaaa attggttgct tagcgctgtg
      361 atttcaatac gatggtgata tatgagaggc acaaactgga ctagcagcaa tcaggcaaaa
      421 gcagattctc tatggaacaa atacattaca gataaatcac tctttctctt taattacaaa
      481 ccagtgctaa cttgcagaat ggcaaatgta tggttttttt tttaaagaaa atacagggca
      541 acaaatgaaa tctcctcttt ccccccttgc ctattttctg cttttatcat ggtgcagtgg
      601 attttttaag ctgacagcac tgttttgtgt gtgcccaaac agcagcagct acaatcacag
      661 tgtaaatact cgcgagtggg cgtcacccgg ttaacccata accacagagt cgtccagtaa
      721 gaggatatgg aggccatctg gtagcatttt tacagtatgg tgtggcagtt tgcccatgtg
      781 tgctacagga agggtcgtat gacggtcggg aaaaagagag agagagagac tctggaattc
      841 tccaaggacg cagacatgaa tattctcagg ccaattttct gggtcagacg taagctcgac
      901 caaggtacac gacctgatcg catctgtctc cgggccgtgc agcgtgaatg atcaaactgc
      961 acgacctcta aaatcttaaa tgatatctca gaagttcttt aagctgcatt tgcagtatgt
     1021 gatgaatcca tttggggggg agaggttttg aaattacagt gactgtcctc tttttttttt
     1081 caaaggcacc atatgttaat aagtaggaca ttttactggg ctgctacaaa gtttggaatt
     1141 gaagcaaaac taggagctga atgtcattat tgtagaaatg aaagaaaaca agagtgaatg
     1201 atcaataact tgacgtttac tttgaacagc atagaaatgc agtacatcag tacatcaagt
     1261 tcagcagttc tgaggtaatt gtactttcct tttaatgcag ctgtgtactt ctacttcact
     1321 acatttcaga gggaaattac acactgtaaa tttagaaaaa a
//
LOCUS       KCVB01000512            1218 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24204pri ultra-conserved element locus
            uce-120, sequence.
ACCESSION   KCVB01000512
VERSION     KCVB01000512.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24204pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1218
                     /note="ultra-conserved element locus uce-120"
BASE COUNT          327 a          256 c          234 g          401 t
ORIGIN      
        1 gggaccttga gtgtgacact caagtattat aaattactcc taatgacaat aatcatgaaa
       61 taatatcctg ttaaaatttg aaacatctaa aatgtagttt taaatgtttt tcctccctgc
      121 gtatgcgtgt gttttttgca tgcatctctc tgtggctgca tgtcagactg tcagcatttg
      181 ttttcagagt tttacacagt gtcacattcc taaagttgtc atattgagtc cactgaggta
      241 cacagtaaaa aacccacagg cataccagtg agcttagaca caccgcaaat atccaccctg
      301 atatacgaca cagccctaaa gtcaggaaat aaaatgtcta ctgagtgata cgattttctt
      361 tctttgtttg cttctttgta tcctttgttt ttctttcttt ctcgcttgtt tgctttcttt
      421 ttttttccca cctccctctt tcagtgtccc tgcagattcc cctgctgtcg gctctcgtca
      481 cattccccaa tctgttagcc ttctgatacc ggggccagac ttgaaaggaa gtcttaagga
      541 cgagagctta tacactgtgc cagattttgt taaataacta cgagatgttg gtgccaattc
      601 atcagtaatt gtcccttctg gcacagtctg ttgaaagtcc tggagaagcc taagctcaag
      661 atcactgcaa gtgttaatct tcttatactt aaggtatcag gttttctttt gatgtcgcta
      721 aacatgttga ctcctatctc agcgcagaag gcaatccaga catagctacg agcgagatgg
      781 gggtttgtct gccttcttcc tccaagaatc tcatggttta tgtgttcctc tgttagcatt
      841 cattatggtg ctaaaataaa gcaacaacaa aatgaaaggg gaaaaatgga aatgggaaga
      901 aagggaataa ttctgtgcta attgtccttt ccctagcaca atttggctgg ctcctgtatg
      961 atgtataata gcctaactta ttttcaatga ggcaaaatcc tatttctcag actatacagt
     1021 ataatagccc aaggttattt ccaagaagtg cttcgtcagt gggctccttt caagatcaac
     1081 agaaaagaca catgtctcaa agtgtagacg atttagtgct ttaattaatt cccctctccc
     1141 aagggtgtcc tgtatatgtt ttaggtgggt atgacacagt cagtcagtgg tagatttcat
     1201 agatagattt catcgctg
//
LOCUS       KCVB01000513            1454 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24247pri ultra-conserved element locus
            uce-1112, sequence.
ACCESSION   KCVB01000513
VERSION     KCVB01000513.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24247pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1454
                     /note="ultra-conserved element locus uce-1112"
BASE COUNT          392 a          328 c          283 g          451 t
ORIGIN      
        1 tgcagaagtt gcattctgga gtaactgctg cgcactctga gcctttttcc tatcccaaag
       61 aaggcccaga tggctgcttg ttattgcata acaaggctgt attgttttgt taatgtaact
      121 tcatctgtct ttgatctaat cacatgttgt cctggccagt gtgaaaatcc ctatcaagtg
      181 catgatccgg cactacgctc attgtgattg tgataatctc ctgcattgaa attccacttt
      241 gttcctttgt ttctttgtta gtgagcacaa tgtgaggtta attagcagcc actggtaacc
      301 tctgcgttcc tggatccttc atcacagctg aagtgtactc ttcaccaggg ccgctggctc
      361 tagcaagacc aaacacaagg ccttgtacca attgaaagta agcagaacac aaacgcggca
      421 gcaacttaat ccctttcaca ctaacacgca ggcctcaaaa gtatcaactg taattgtttc
      481 ttcacgtcat taattgctct catcattgtt tcaactcttt gactgaaaac acacagacga
      541 ggaacaggcc caaccagaag agcagcagga tatgtttgca gaaaaaggtt gcggtgatca
      601 agtttgtcaa acattaatag gctttaatgt ggtttttatc tgattctgag gtgtcacagt
      661 gtaatttctg tttcttctgt ggtgcagaag ctctcaacac agtgcattta tctgcaatat
      721 tgcccttccg ccacttcaga tctccctatt ccctggtggt ctaatttatg ttaagtcaca
      781 aacctcccct atggctgttt tgaaatgttc agaatggaga ctgtgtgctc caggtttatg
      841 ccagcaccac aatcctctgt ataattgttc tctatgggaa agcacatctc agtgaaacca
      901 tctgcaagtc catggggcag tttcctaaga atgagtggac ttcttagggg gttccactgc
      961 aatccagttt ctcttgtgaa gcaagtaatt aattcaaatc tcaggaagtc aatagctgcc
     1021 cggatcagag gcacgtagca cacagctgct gatacctccg aacacactgc ttgtcttctc
     1081 aggaagttgc caaacaaaaa ggtcacaatg ggcgcacaca cccatacaag gctcagataa
     1141 cactcatttt aaaactgtga tttttgtgta ctcttatcat acgttgcaca cttcattaat
     1201 ctacattgta gtgatgatca gagtcaccaa tgctcttggg aaatgggctt ctttatgaat
     1261 gatgtaatag accgtcatta aacaagcttt caaggctctg tggttcctct agctacaatt
     1321 attttctctc acgttgattg tttttttttc ttgaggttta tagaaaaaat aaccttctgc
     1381 agaatatcca accattatgc aaaacattga ttttaacttg gcagggtctt ggtttcccaa
     1441 acagttgata tcat
//
LOCUS       KCVB01000514             809 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24289pri ultra-conserved element locus
            uce-1157, sequence.
ACCESSION   KCVB01000514
VERSION     KCVB01000514.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 809)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24289pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>809
                     /note="ultra-conserved element locus uce-1157"
BASE COUNT          252 a          182 c          177 g          198 t
ORIGIN      
        1 gctgccacaa gcctccagac tcattggcaa tgcatgacca atccccagct caaccgcccg
       61 aacatcacca gagtttagca cttagaacat gacagacacg tccggaggct tcatttccac
      121 acagtctgca aacactggga tcatatggag ctcagcatgc agcgtctgat aacatcttat
      181 taaaggtagt atcagataaa atatactgta ttgtttcaca gcacaattat caaacaatat
      241 catattatat ctgatagaaa tcctaaacta ttgagacaaa caagataaga tttcttccag
      301 tggaagcagc ctgaccagat ctggacaacc aatttgactt gttccccctg caaattgaga
      361 tcccttcacc tgaccccccc tgaaaaaacg ggccttgaaa aggggctatc agttttaatt
      421 ttccaaactt tctcttttcc gtcaaaattg ggtaggagga aaaaaggggt ggtggggagg
      481 ggggggaata gaggcagaga ccaccattcc aagttttatc tggtctacaa aatgccgtta
      541 tcttagtgcg gtgtcaagaa ctctatctct ttttttccag gtgacaggca cagacgagag
      601 ataagattgg aaaagaatcc cactccactg agtaaataaa taatcaatac tcatctaaat
      661 gtaaatgaga aagtagcccc ctttgatagg agggttttta tcaaccaacc aattttctac
      721 aggcactcac tgggcctcaa ccaaatcacc atcattcagc tcccatgtaa tggataagaa
      781 agggggttgg gggggggggg ggggggggg
//
LOCUS       KCVB01000515            1473 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24330pri ultra-conserved element locus
            uce-648, sequence.
ACCESSION   KCVB01000515
VERSION     KCVB01000515.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24330pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1473
                     /note="ultra-conserved element locus uce-648"
BASE COUNT          366 a          368 c          191 g          548 t
ORIGIN      
        1 ttttaaatct acagaatcct ttggtaagtg cttagctcta aagcacacat caccccccac
       61 taatttccat gtttcaaaca actattaaaa tgcatggcat gcattttact aacagatagt
      121 ttagtttcaa ggctactact agagacacac acactcttcc ctcaccctaa ggtgttcaag
      181 tgtcctacca ctgccagctt tagtatcacc tttatctgca gcttataacc accaaactct
      241 agtttagtat tgtcatatac tgacctgggt tatgacctgt cctcacatgt ctgttactca
      301 ctcactgacc caccacttac tcacttattt aatcctttaa caccagcaca gtaggtatgg
      361 gaatttcaag agttttaggg aggtgaccag ctcagatcaa gcaaagggat ctcagttttt
      421 taacatgtct gttcacaatc tcatatacaa agtcctcaaa gtttttccct tcagtagatg
      481 gtcttcagaa gccattgcca cttccaaaat ggcacctccc cgtggtacat caatgtacaa
      541 gttcttctct ctgacctatg attttcctat gatgaatcct gtctttctct gccaatagag
      601 tacagaaaag ttcctattct cttttctctc tctctctctc tcctttctct ttcctctagt
      661 acctttattt acgtaccctg ttaaatctaa tttgattctt cttccaaagc ccccagcctc
      721 tatctactcg tactaatgct tcttctttct aatcttattt gcattcatat tatccaccca
      781 ggtggtacac caataagagg taagagtgct gaactgacgt tcacagaatg aaaataaaac
      841 aaattcattc ttgctgtcaa cccatccatt cacttgaatc acctccattt ttgtcctgtt
      901 tagcaagacc aaatcccatt acgttcatat ggacctccct ccttatttgt cctgtttcgt
      961 ttttctaaat ttgtattcca tctttttgtc tatgctttat ttactctttt gtattctggt
     1021 ttcaagagcc tgtttagctc agttgtgata tgcttgccct ggctcaaata agcatatcca
     1081 atgacaatta ttttttaaac tatatttttt ccgttcattt ttgttttctt tttggccata
     1141 gcgaatcatg tgtccctctt tttgtccaac taaaacaacc aagcattttt cccccttagc
     1201 tgttcttgtt cagcactaaa aatatgcaca gatgctgtgg aatcacgcag gcatgctgaa
     1261 gacaacattg cctctcctct gcttttttct cccaaagaat tctttctatc tttgcaaact
     1321 attttttttt ccatttcttc tatttgcttt cctctcctgt acctctacaa aaaagcccaa
     1381 acaaccactt ttttgataaa gtttctacgg tgcttttttt ctctttcacc tatttttctt
     1441 aattttcttg ggtttggaga ctattttact ttt
//
LOCUS       KCVB01000516            1625 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24369pri ultra-conserved element locus
            uce-299, sequence.
ACCESSION   KCVB01000516
VERSION     KCVB01000516.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24369pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1625
                     /note="ultra-conserved element locus uce-299"
BASE COUNT          397 a          397 c          363 g          468 t
ORIGIN      
        1 ttcaaaaatt gaagttgatg aacatgacgt agatggtatg tatctgtgat tgttacaggg
       61 agcgtggtga caaacttcca tgtgacttgc ctagtctgtt cttaattaag cttgactgtg
      121 ttataaaaaa aacgtctcct gtttgaggtt ccataagcaa tgacttaagt atcatatttc
      181 tccagctcat ggtcatacat gaagtcatgc acagttgctg taccaagtca cattttcctt
      241 taggcagcag gggaaacatt taaattccac atattcatct gtaaacatta tgaatctaaa
      301 ataagcctga attttgaatg tcaattggtc attttaataa cctgcttatc gagttgcatc
      361 ttcacatcct tcgcttagca gcaatatgct gcagaggagc atagtaatgc actcggtaaa
      421 aaagcataaa taacaaaata aaagcattac ttctggtcct tgagtgcagt gccaacctct
      481 ttcagaatgt tttaccctga tgggtattag cccgcaacct tctgctgcat tcaaaaaaag
      541 ctgctttctc aaactgcttt cccaaatgct gttacacaac ataaacatga atatacatta
      601 tgctggagtt gttgtctttt tttctatttt atcccttttt tttatgtccg gttcatgttt
      661 aattagaggg gcataataaa gtgaagtcac ggtatgcccc tcctctggag tgattgcaga
      721 gggggcggag gaggaatctt aacttatttt cacttcgttt tcacctcctc acatttcttc
      781 atttcacatg ggctggaagc gcctgcagcg ttctctttcc cttctccaga gcaaagtgaa
      841 tgtgactttt ctaagggggt cacaggatgc ctggaggcct gtgggtgtta ctggactgtt
      901 gcctgctctc agcttcactg aggggcttgt catagatttc atctccacag aaatgagccg
      961 gggggccctg gccaggagct caagcagcca acgttatgaa gggcccgtgc tgattatttc
     1021 cctctgacat cgcatcctga gctttccgca ttaatcccag acaaaggcag cccttcagtg
     1081 ctgtgccaga gttcaaatta ggagccagat aatggccttg ttgagatcct gctctgttca
     1141 ccgagaggca gagctcctgt tgaaatcctc ctcttcagta ttccttccct actgcttgtt
     1201 cttttcctca tcagaacacc ttttccacac gtttggactg tgacggaatt caggtgacac
     1261 aatttcgctt cattcctccc caacacaacc cctctacccc tattcaccca cctttaatgt
     1321 gatggtggct tggccaggct tgggtctgtg cctggatgtg gatcgccaca ggctgtgaaa
     1381 ctgatagcca gtcaagactg gagtgtctgt ctccagcctg atctagtctg caggagggaa
     1441 caaaggaggc cttacagggc tccgcagggt ggattagttc caaggagcca atggctgcgg
     1501 ctgctggctg gctggctgga agagggcctg ctgtggcccc agcggttggt cactgcacag
     1561 gcaggggccg cacaggcaca cacaccacaa acccccctcc agctcaatag tccccatcct
     1621 acagc
//
LOCUS       KCVB01000517            1036 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24409pri ultra-conserved element locus
            uce-158, sequence.
ACCESSION   KCVB01000517
VERSION     KCVB01000517.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24409pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1036
                     /note="ultra-conserved element locus uce-158"
BASE COUNT          274 a          201 c          229 g          332 t
ORIGIN      
        1 atgatcttaa attgtagact tatgcaatgg ggagccaacg tgtttcaaca tggagggaca
       61 tcaggaaaag cttccagctt tatgaaggtt acttaatcag tgaattactg ttcaaattat
      121 attaaagatg agagcttggt ttttggattt attttgggtt tatgtgaaac caataaagga
      181 gtatttacaa aacatgagca aagatggagg aattgtgaat tggagttgtt tgcataattc
      241 agacggtcca ttcagtttta acagcaaagt ccattgttat gtgtctcttt aaaaatccaa
      301 ggcaaatgag tccaacttgt gggcaattta ctctgttgtc atgtatagat ggtgaacgta
      361 cccctctctc tctctctctc tctctcactc tctctctctt tctgcaagct ttgctgtcgc
      421 agcagttgct gtcagtttga gcggtgcctg cccccaaata taatagggtt ttcttgactg
      481 cctccctggg gattgactgg cggtgaagaa ttcaccatgg ctcatttgga gcagtgcagg
      541 gacgggcttc ataaaactag ctcctccatg tctccaggcg gttctgtgac attccaaaga
      601 aggtgttagt ttcactgata atactttcat ggaaagaaag cacttagatt taccatgttg
      661 tccactgtct ccagctctgt ctgcggcgca tgtcacaagg ctggagaatg agactgtgga
      721 ctgttgatgt aggtgctgtt tcaaagactg tacgtcatgt aaatgaggtg tctattaaaa
      781 gcactgtaga tctcaagcaa ggatgaatga ctgtcacggt gcgctgcttt tcacaattgc
      841 cacaaaaaca atgattcatc gctgaggtca tgatcaatta aattgatatg tttccatttt
      901 tcaagtcaat tttaaagaga tgcttacatg tctatatgta cactgaatta gttataggtt
      961 gctgcagttg ttgctacacg ttctcactcc agactcgtca catatggaca cttcgtcagg
     1021 actttttggt gtcact
//
LOCUS       KCVB01000518            1573 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24452pri ultra-conserved element locus
            uce-1296, sequence.
ACCESSION   KCVB01000518
VERSION     KCVB01000518.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24452pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1573
                     /note="ultra-conserved element locus uce-1296"
BASE COUNT          442 a          362 c          304 g          465 t
ORIGIN      
        1 tgttaatgaa gtggtttctt gcatattttc tgcgtctggt ttctagattt atgaggtcac
       61 aacagttttc acaacctgac aacccaagaa ctagtcctat taatagttat aaccgatcta
      121 tgaagaatta gtaaatgatt tattagtcat tattaaaggg attagttaca acacaaagcc
      181 tttataaagg tgttgtatca gaaagaacta aatttgttta atctctatat tgcaacaatc
      241 ccagagttcc taaagactct gattcggtgc acatgcattc tttagtgtca tctgatcttt
      301 gcatctctcg ggatcccacc gtcctcattc ttctcctgtg gtccaataga taaactgtca
      361 ccttgtttga taaatgaagg gcagcggtca acataggctc ctttaacatg acaagagaga
      421 agtattgaat ctgcaatgcc ctggatacag tcaacataaa acatatcaaa tggcattcct
      481 cgccacattc ctatcaatat caaaaggtgc gtgtttgagg atatccttag tgcgtttacg
      541 cctgtgtttg cgccctgacc cactggggcc agtgcaataa ctctggactc cctcccacac
      601 tgcatctcgt tgcaatggcc actctcagtt tttatttagc tgggttgtgt gccttcccag
      661 tccagccgct tttcagattc atttccaggt aaacacagtg tggccagcca caaacctgca
      721 gccaacaggc gttgtcacag ctgtggcaga taatggcaag tccaaactcc agcccggctc
      781 tggaatgttt actgtcacca caggcaagtt ggcagcaaac attttttttt ccagcacttt
      841 ctaagaggaa gactctcccg ttattaattt tattttgatg aattacatgc ttaacccttt
      901 gccctgtgct gttttgtgtg actttgtgcg ctcagagaaa cacgaaggca ccgaaaaggg
      961 acatcctagg tgaatcttta aatttctgca gaaaaaaagg aatccttact ttgaaagttg
     1021 aaacttgaag ttaacacatt cccgccaaca actcctgggt gtttgaagga atgttccaag
     1081 agttccgcct tcctctcaaa gagcagagag caccctgttt ccatccccct gccctgcacg
     1141 caagaacccc cccccacaga cacccaacag catctgtacc agaaaactac tttgtggtgt
     1201 cactttaggg ccttgctcac gcaggtgtta tgctttcact tctttaactg actcattaaa
     1261 cctttatcat ttagtgtttg tccttgtcat aaccagtaac agaatgacac ctgaatgtgt
     1321 gaaatataac accgatcatc atggaggcta attgatcaac aaggtggaaa aaacaccctt
     1381 gaaagaggag agacgtgagg cattacgctg ttctgggtta ctgaatatgt atattttgca
     1441 agaacacact gtactatctc atttttttcg actcatcctt gtgagagaat gtaaatgcag
     1501 ttctcttctc atttccaatg tatgcacagg gttgtgaaag cttgagcagg gaacagaatg
     1561 atcaacaata aat
//
LOCUS       KCVB01000519            1347 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24553pri ultra-conserved element locus
            uce-688, sequence.
ACCESSION   KCVB01000519
VERSION     KCVB01000519.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24553pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1347
                     /note="ultra-conserved element locus uce-688"
BASE COUNT          389 a          239 c          316 g          403 t
ORIGIN      
        1 tatttctttt tgactgtacg gaagtccaac caatccagtg atacagagaa caacgatgag
       61 tactatgata aagcataatg cactgtgata cacagactta atctactaaa cggttgatgg
      121 gacagcgtgg caatacagag catctcatca agggcagaca tgatggttga ctgcacaata
      181 gttttgggcc ttgtttgttt ttattttttt gaagtgaaat gttaaatatg aatcttgtaa
      241 gaaagtcttt tgtcaagaca aactcctaag tttttgtaag actgaacatg agtaatatgc
      301 cacagtcagg tggagagtcc catgtctttt ctgcatttaa aacaagtctg tgattaagga
      361 gtgataattg gaaagcatca aaggcagact aaggtcgagt cagggccttg gctggagcct
      421 cgtggatgga tgtttgaaat attaaaaaat ggctgatggt ttcatcttac atcttaaata
      481 agatgtgttg taagcttatc cataatgtga aataaatgcg atgctgtgtg acaacaaatg
      541 aaataacagc ataaacaaaa acacaatatc catatttcca cacttccaca aaaacagaaa
      601 tgtgatatta cctcccctct gtgctgctga tgtgcatctg cctaaagatg atgaaatcat
      661 tattgtgtgc acgagtgtgt gtgcgcacaa ttgtgtgcgt atgcgtgtgt gtgtgtgtgt
      721 gctgctatcc aatctggatc cctggacgca cacgtgcaca ggcctctctc ctccaattca
      781 cctctattac tagctataat cccatttcac actctatcga tccccaaaac tgttgttcac
      841 cggaaccagc agcaaacact ggcatttctc cccatcattt caccaccatt gttctttatt
      901 acacaaagaa gccccccttt gtctcagagc aaactcagtc ctcctgaaat atgaattata
      961 aatgtatggc taataaaaga gaaggggggg gggagagagt gagagagaag gggagaaaga
     1021 tagagataga gagaagtggt tggtagaaac atcagagaaa atgactcctt tacaagagtc
     1081 tattgatcag tttctatcaa agggggatgt cagacatgag attacctggc agtttgagca
     1141 ttaggtgccg tgtgttatag ccccgggccc ttgtaccgct gccagaggat tgtaaataaa
     1201 gcggagagga gagagcaaga ggggagtgtg ttggggtgta agtgtgtgtg agagttttag
     1261 attctacgtg tgtgtgtgtg tgttagtctt tgtctttgtg agtgttcgct gttgtgtgca
     1321 tacgtttttt tttttttttt ttttttt
//
LOCUS       KCVB01000520             917 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24636pri ultra-conserved element locus
            uce-574, sequence.
ACCESSION   KCVB01000520
VERSION     KCVB01000520.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24636pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>917
                     /note="ultra-conserved element locus uce-574"
BASE COUNT          256 a          231 c          216 g          214 t
ORIGIN      
        1 acacacacac acacacacac acacacacag acgcgcatac ttcttacatt agcgcatgca
       61 cgtacagact cacacgcaca cacagacaca ctgatggctg ctgcggtttg ctggtgtaat
      121 gtgctttgtt gtgtgaaggt atcaatcaga ggggagcaga ttattagagt gtctgaaaga
      181 gatcaaacaa tgttgccgtc tgcacccaca catggctgat ttacaggcag gctgataaag
      241 tactgcagct ctctgtcagg ggctacatga cggctcgccg cgctcttagg ataaattatt
      301 gcagaggccc gcggtaatgt ctctccacct agcaaccagc cgccccagcc gctgccaggg
      361 gagccctgcc tcgtctgcca taatggcact gtgtccgggg tcagcagtta ctgtatcgta
      421 gcagggtggg agggatacat acacacacat atatatacac acacgcttga atgcatgcgc
      481 gtgcacgctt gcgcaccgtt tctcgtttgt ctctacacct gccattggaa gcaatgccaa
      541 cagacactca gggatgactg caggaggact gagtggacac aggggaggtg ctgatgtctc
      601 gctacaattt tgcagtagag caacattttg aaaaactgac gccaaggttc caaacaagac
      661 agtgctcaga ggaggtgggg gcaccattat aaaaaaataa ggcttggcaa aaacgttata
      721 gaaagccatt tgtaacacta agtccgatcc cacttacgtg attggccaag aggccacttc
      781 tacagagacg ttctttcata ctttgctgtc tctaactgtc tgtccttatg tatgcagacc
      841 agcactaaaa acaaccactt cctgattgat aagagcctaa tagggacatt tttatacact
      901 agtgttttta cgacaaa
//
LOCUS       KCVB01000521            1459 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24677pri ultra-conserved element locus
            uce-301, sequence.
ACCESSION   KCVB01000521
VERSION     KCVB01000521.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24677pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1459
                     /note="ultra-conserved element locus uce-301"
BASE COUNT          460 a          286 c          283 g          430 t
ORIGIN      
        1 gcaaagtagg ccgccttcac tcgctgtcac aggaagagga agtgtcttgg tgtaccttca
       61 cctatgctgc tgcctcgctg gtgctgccgc tacaactcgt tgcacggctg gagacgagac
      121 agcgagcgtg ttagatcaga gagcagcgag gctcacgtgt tgcatttcct gctaccgcga
      181 ctgtgtcgag tgaccatctg agaggtgtca gaagaaaaga gccgcagaga agatggctct
      241 acctcaactg aacttactga ctgctctttg aacatatgat tcatgttcat tacactgaaa
      301 taacacttac agcagagtct aaacactgaa atttacattc tgctttttta tacatttaac
      361 ttaaaatatt cctcctcaaa agagcaaagt aacgttcaaa caccatgaat gggtggataa
      421 gttcatgttg attttaaaag aaaagagtca ggtaagttgt cgcaaaacgc acctcgataa
      481 tgaacctaaa tcaatttagc aactaaagta gtggaaagaa aaacaggttg ggattattat
      541 atatatttcc ctacatttaa catgccaatg tgcaaatccc acaaacatca gagatctggg
      601 atggcgagtc cttctctata aattctataa atgtctggtc tcttttcatg gattactgtc
      661 atttgccatc cgctattgtg agcgcctgcc aggctggctt gccatttctg ggcctgtgtt
      721 tccaaccttg atatatttgc caaatgaata tttgaagctg taattaaatt tatcccacct
      781 ctacaccttc tgtaagacgg ccaccttgct catttctcac atgccatatt tcagctattt
      841 tttgtgtgaa aaaaaagaaa aaaaacacag cttgttgcca ttttttaatg atttggaatg
      901 aggatatttt tactagaata aatctgattt caggacattc ataatggtcc tgcttacgta
      961 ttcagacata aatacgcatc gtgtggtgag aagagccgtc agagctgtca ctggcttcat
     1021 aaacaaactt cataaaatgt ctgtaaatat gattaaaaaa ttaggagatg aaattataac
     1081 agaatttaat gcatcaagca agaatcaggc tgctcctcac aaacaactta ctatttccag
     1141 acagccaaga gaaggcgctt aaaatcagca taaatgtgtc cacatgaaag aataaaactc
     1201 ttctcatttc tacacgtctg cagctgagtt gtttttttct cacattttaa agggacaact
     1261 gaaaaagttt attgtttatt taagtaacag aaagcacaag ctttagactg gagggaaggt
     1321 gatgatttat taataaacta catacaagag gatgcttaaa ctactgttca ttctgagaca
     1381 cgaggccttg acgcgtgctg ggaacaaaca tttgattaat tcatgataag gaacaatgaa
     1441 gctttgactt ttttgtttt
//
LOCUS       KCVB01000522            1509 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24719pri ultra-conserved element locus
            uce-344, sequence.
ACCESSION   KCVB01000522
VERSION     KCVB01000522.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24719pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1509
                     /note="ultra-conserved element locus uce-344"
BASE COUNT          421 a          336 c          334 g          418 t
ORIGIN      
        1 aaaaaaaaga cattgtaaga ctgtttttca caagcttagt agcatttttt gtgacataca
       61 aactttttaa tgtaaaacca gcggagttct cctttaaaaa caaagaagac tcataattaa
      121 aaacggggat caaacgtgat gttctgcttt ctgacagaaa ttaattacta cttcctctca
      181 ggactgtgtg tgtaacaatg agacaaagga cgtctgttga ccttcctgtc agcgtaccag
      241 agccctgctg aagagcaccg ggcctctgca gtggtgggtc gtagcccagg tgatgaactt
      301 ctgaacgtgg tccagctggc cacatatctc tattgccccc tgcagctggt cctccagacg
      361 gtgctggaag ccctctgaga ttttctgcaa agaaaaaccc ataaacacgc atccagttac
      421 atgtaattac tggtacactg ggagtcatgg ctggactctg gactaaatca ggggctcttt
      481 tttttttgac agatgtagtt cctgaggagt tagcttaagc agctttgaca gattattatg
      541 ggttctcatt tgcttgcttt caggactgat tgcatcacgg tggtcactga ttccttgcat
      601 gtagattcac ggctggccga acgataagaa ggaaagaaaa agagagaaag taattacctc
      661 cagttgctct attaataggt tggctcgttt gttaagctca ttcatcataa tcatctttgc
      721 cattttaatc tggttctctg ccttcctgtg tgcaatcttt actccatgaa gcctatgaag
      781 aaagaaagga gaaaataaat gcctcagtcc agaaaaattc caaatgtaga cgaagacagc
      841 agcatttcgg acactaattt atgcattagc ttgctatatt tcatgctgtt aaattattta
      901 ccatttgtgg cagaagttac tcagacttgg gactgtgatt ctgtgcgagt ctaaacccac
      961 ctgtcctcta actgttttgc attgttctcc actgcagacc tcttctcttc cacctgcacc
     1021 atcaggctct ccaacagcca ctgctgatcc tgcagggcct tcccgatctg caccaccctg
     1081 aacatgtgag agatcacaac atccacagga cagaaagttc cctaatttat tctctgggca
     1141 tgaaaaacac cagaattaaa tttgttctcc ctggggacag gacattatgc aggtgacctt
     1201 agaggcagct actgtaccaa aacatcacag aaaacaaacg catatgaata cttaataggt
     1261 attaggtggg ccaaggagag cgtatgcaga tacagatgaa gtaagtgaca aggcagttaa
     1321 agcgagttgt tgctcggagg aagtttttaa cctacggttc gcccctctct gcagtgtcct
     1381 tgtgctgtgt atctgcagac tgacctgtgg tttttgtggg aggacaggtg acagctgcta
     1441 cagcacagaa ggtcacatga ctcacaaaac agctccaagg gctcctgtct gtgagagtgg
     1501 cacatgagg
//
LOCUS       KCVB01000523            1009 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24760pri ultra-conserved element locus
            uce-795, sequence.
ACCESSION   KCVB01000523
VERSION     KCVB01000523.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24760pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1009
                     /note="ultra-conserved element locus uce-795"
BASE COUNT          273 a          284 c          201 g          251 t
ORIGIN      
        1 ccacaccggc caacaacaac tcaggcagtc agtcaagctc atgatgccat gcggtgtcat
       61 tccacaacac tgcatgtttt acatacacac cgacttcaac acacactaac atacacaccg
      121 acacacacac aaacacacac agggcttctc ctctccaccg cactccaact tactgccagt
      181 tagggagttg tcttcctctc gtcttgattg gcagaaatta aacaagatgc catttcgctc
      241 aacctcctcc ataacttcct ctcgcatctc ccggtgagga agcctcaact tgtccgggac
      301 agagctcccg acgccggccg tgaaacgtcc aaccggcacc ctgcgcatgt ttcccccctc
      361 cccggtagtc ctcgcggttt cgatccggca gccggcgcgc tttggcccgc gtgcagcccc
      421 gtggagcccc cgctgtctgt ctggagctcc gctgtcttct ttccgcgagc cctgcaacaa
      481 gaaaagccgt ttatggcgac acacaatgtg aaaagggggc cgatttaaaa aggagaagaa
      541 tgcaagcaag aacaacaaaa gtcagttata gcaaagtcta tgcctctcct ctcctatctt
      601 ccacccccta gcttagccca cataatgagc aggaaaaagc tttatatttt gttcactggg
      661 tattcacaga taatttgaag ccattgtttc tgccacagat ggttcttttt aatacaataa
      721 cggtgctctt tgtgaatagc gagcccacaa taaaaatcta aagcgtcctc ctgaataacc
      781 attttgttct ctcttgtata ggccgaacaa aagcgccagt gaggtaatgg cctgccttat
      841 tgaaagcctc agaatgggga tttgataaag atttttttcg ctcctttttc cttttgcaca
      901 aagtacacgg caatgtcaaa gagaaactga cacggcatga aaaggaaaaa ggctcgaaaa
      961 agtgcttgct gtcgcaggtt gcaactatta ttattattat tattattat
//
LOCUS       KCVB01000524            1468 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24802pri ultra-conserved element locus
            uce-469, sequence.
ACCESSION   KCVB01000524
VERSION     KCVB01000524.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24802pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1468
                     /note="ultra-conserved element locus uce-469"
BASE COUNT          462 a          244 c          252 g          510 t
ORIGIN      
        1 ctgtactata tgagttgtca ctgttctttt gtttgagctt gtctttttca tatgacaact
       61 tattatgaag tggtggttca gttacttaga aaaacttgtg aggaaactgt tcaattgttg
      121 tcatgttgct tgtggagaaa aaaaaagatt tggattaact ttcagttagc taccgcggca
      181 aaagaaaccg aacaaaagct tagaatataa ctgtatttgt ttagcagttt atttacttat
      241 ctatttttaa actatttatt tttctattta tttgtattta ttatatattt attaattaat
      301 taatttatct gtgtatttat gtatttattt gtttcattgt ctgtgtattt gcgtgattct
      361 ggtacaggga ggggacgttt ctgagcaaaa gggaacaaag cgactagtgg agaggctctt
      421 ccagtagtcc actgcactga gagaactttt agtacgtttg tgctttgtac caatatatca
      481 aaattacaat ataaaaaaat ctaaaagtca agaaaactga aaaaaatgtc tggagggttg
      541 ggggacgctc ttcccttatt actagaaaca gttactcgct atgcataacc tagttaatag
      601 ttaaatttgc tattgctttt ttcttgtctt gttaaaatga aatctttaga tctttttcaa
      661 taaagtttag tcttaataga atgttataaa cagtcgttat ggttcaatat aacctgtgat
      721 aagtttgtgt agttctaaaa agccactccg tccacaccct ccctccactt actaccgctt
      781 tgtgatgaaa ccttatgcaa aaatgtgggt ctgaaagcat agttttcagt tttcagtaaa
      841 acattttata taacattcag ctcttggagg cttgggcaga tcaattttaa aaaggagcaa
      901 aatgcccatt gttgtttgag agtcattcat tgcactggac taaaacggtc agaatcactt
      961 aagagtcaaa acttattttt aaaaaaatat tcatcatgtt ttcaaatgac ataccaacaa
     1021 atctttaaaa atgagaaatt gttaccattg cacaccaact gttatttaca gcagttgttg
     1081 ctatacttaa gaagtactct ggaggtatga aaagggattg taccgattct atattttata
     1141 tactctaata tgatatatat tatatatttt gaatggtgaa aggtaaggga atggttccag
     1201 aagtataaat atacggaatt ggattttttt tgtttgttgt taagacttga aaacatgaag
     1261 gaggtgtgta gagagcattt taaacactca gacgcatgct cgcattaagt acatccttct
     1321 ctctcacacc tcagtcatct gacaggccgg gtcatcttat atcatcactt attatccagt
     1381 catcatctgc gccatccagt cacatagttt tctaacgtca tgacggcaac aaaccaacca
     1441 accaacccat ccacccacac accaacca
//
LOCUS       KCVB01000525            1650 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24843pri ultra-conserved element locus
            uce-1333, sequence.
ACCESSION   KCVB01000525
VERSION     KCVB01000525.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24843pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1650
                     /note="ultra-conserved element locus uce-1333"
BASE COUNT          444 a          362 c          386 g          458 t
ORIGIN      
        1 agactctctt tgtcatgatt aaggacaaat taatagatgc ctcctggtaa tcagctttca
       61 ttagggctgt caaaccgtca gtggcgttcg ctcagtgcac tggacacggg atgatcacct
      121 cccagttgag agacactaca gctggctgtg tgctgctgtg gtactgcctc tcagctgctg
      181 tatttgtatg cttggtgtgg ctaaagtgtg ggtaaccagt gcattagcac gctccttcgt
      241 ggcacaagtg cttgtagttg gtgcattaac agtgctccgt gtggagctgc aagatggagg
      301 tcatcgtggg aatgacataa agagtagtct ttctgctggg tgtaattggc cccagcatgc
      361 tgaaaatgga ctgtgaaatg taaggggggg tcaaataatt gattttcccc cgaatgcaga
      421 atccagggca gggctgttta gtgagtaatt tacaagagca gttcagccca ggctgactgg
      481 catatctgtt gaaagttccc aggggcacag gaagagataa cacacacctg agaccctgga
      541 gcagatggac agcagtcact gtgctactca gagggcagag aggatccaca tcaaacttac
      601 ttccacacat ctgcgtatgc agaggggatg taagtgcaca tatacacatg cacacacaca
      661 tatactcaga ggatgatgga cacaggctat tgtatgtaga aatagattct ctcctcttac
      721 tctgtccgtc tctctttctc tctctctccc tccctctctc tctctccctc cctctctcgc
      781 tgcagcattt ctggaattga tgaaataaac tcatcagact tccagcaagt ttggcattgt
      841 gtgagagagc tgaagtcatt tcagtatgta gatttcagac ttaaacccat aaccgtgttg
      901 cgtggtgcat gaccaccccc acctgctgga gagaggaggc tggggtgtgc atgatgggat
      961 agcgacgtct gtaagacagg atatagcctg gccctctgaa tgtttagtga tttttctcca
     1021 ccacagccta aaggaattaa gacaatagcc ttatgtcaga tagcataatc tactgacaaa
     1081 atggcttttt atatgcagcg ccaaaattgg gctcaaatac atgtgtaagc taattgagaa
     1141 gaaccagctg cttagccatc tgcatgggta aaattggttt catcttcaat ttggagaatt
     1201 tctcgttaag taaaaagatg agtgacgatt gtcaagatcc atgatctctg taatttgaat
     1261 ttatggaaaa cacaatgatt aaccattgtt gtgttgtgca agagagtcat tgctgtcaga
     1321 aatgtagatg agccacctcg agctcctata ttgtgctcat cacaacaaga catttgtatg
     1381 ccacacagtc agtgctcttc aattttctta agcatcttgg taaattgcac caaacaattt
     1441 tcaagatcaa ttccagcgtc aaaaacaatg cacacaaatc ccaatcttca acccatattg
     1501 tgttggagag ggcccctgtg tttgcatgaa tgtaattaca gtgggatttg atgttgtggt
     1561 gtgcccagaa ttctccagct ttagatcagg cacagggtgc tccaccccat taatactcca
     1621 tatggggaac ccagctgtga tcagaattgg
//
LOCUS       KCVB01000526            1755 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24872pri ultra-conserved element locus
            uce-843, sequence.
ACCESSION   KCVB01000526
VERSION     KCVB01000526.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24872pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1755
                     /note="ultra-conserved element locus uce-843"
BASE COUNT          519 a          387 c          323 g          526 t
ORIGIN      
        1 ctttttttat ataatagatt ttaaaaaaac tcactgaaat gtcaagaaag aaaataatct
       61 ttaccttttt tttctaaact taaaaataca gctgtacttt ctgaattcaa attgctctct
      121 tttatcaaac tgagacaagc ttttttgcct tgttgactct tttgaaaccc actaaattta
      181 gactcagaaa caaaaaaaac ctcaccgaaa ccatcttggt tagtgtttcc tctgtttaaa
      241 taatcaccaa ctctggtttg atttaaaata aacctttcac agagttagaa gaaaaatgtt
      301 ttccccgctg cctctcgccg ccgtagctaa cctctttgtg ctgcagggtt tagctcactg
      361 aacgagagtc tgccgctgct ctcactcgtt cagcgggtgt gtgcagatca aacgagttaa
      421 gagtcgcaag ttaacctggc ttttatgcac aacggcacat gcaccataca gaggcaggat
      481 tttagattca cttctgcttg caaatcaatt tgttcctctt gaagcgacat tcaaaaagaa
      541 caatttgatt tctaacatca ctatcacaca gagacatgaa tccaaaagct tcctttgtca
      601 ggtgcatttt aaattttcca aaaccgaaat gcattgttct ttcattttca aaaaaaaaaa
      661 ttacaaagtg gaccgaggta ataacattca aagctctaat agtacagaga gaaaaaggga
      721 atgactggtc tggcaaatta aatctacaag agcctctgtg tacccagaga ccctcacagg
      781 ggcattttct cctcggtggc cttagagcac ccttcctcct aatggaaggg aagcagaaca
      841 gatgggacac aaattgcact ttcatacacc tcaaggttca aggcaatttc ccctctgcgc
      901 ctgtgaagtt ctcggattgc ctctccatcc tttgagcctt tcactcttca aaggggtttg
      961 ggtttgttca gtgccctgac tcatacacac acactaaaac atactctaac accaacacgt
     1021 gaacacacac acacacacac acacactcac acaggcgcac tatcctaaga gcagttttca
     1081 cattggtcac cctatatgtt ttcatcatca catagtcatt tgtgtgactc attttaagga
     1141 ggacactgat accatcaaag ccatcaccta aataaataga aagattactg attttgtaaa
     1201 agaaaccttt gtttggccct gcagacgtgc acactgacag cactctgatg tcttctataa
     1261 atgcaacttg cttttttttt caagtcataa catgagcagc atatttttca gtctgtaatg
     1321 gcttcagtat tgttgaacgt attacaatac catcatgtta ggtcgctatc cgtctctgtt
     1381 gcccaatctc actaaccacg atagccaaaa gaattgcaat ttatcagcgc gagtagattt
     1441 cctctctttc atttgatgtc gggcgcacag tgtgctgttg aatggcccag gtgctagcag
     1501 agctgctgag gaggtaataa aagacttcca gcggtattgt ttactgctcc attaacaaac
     1561 aaacattagg gcccatttga ctaaatagtg ctgacagaga atatggtgat caatcaccat
     1621 ggcaaccatc cccattaatt aatattgtgc ttggagattt aggaatgctt ggatgaggag
     1681 gacggagtgt gcacagattt ttttgtcttg tatgtgtgtg tgcatgtatg catgtgtgtg
     1741 tgtgtgtgtg tgtgt
//
LOCUS       KCVB01000527            1731 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24907pri ultra-conserved element locus
            uce-494, sequence.
ACCESSION   KCVB01000527
VERSION     KCVB01000527.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24907pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1731
                     /note="ultra-conserved element locus uce-494"
BASE COUNT          551 a          330 c          314 g          536 t
ORIGIN      
        1 ctgaactgga agacctttct tcttcctcta ctctctcagg gtaagctggt ggctctaatg
       61 agttaaccga aacacaacaa cgccaccttg tgtattgtca gtaggtccct gtttctctta
      121 gcctgacaat cacgaaaatc acgtttttaa attgtcccag aattttgttg gtagggttct
      181 tgtatacaga cacaaactct acatttagaa atagtgctga ttgttacgaa aattgacaaa
      241 aaatgactta aaattaagac atttcttcat ttagaaactg caaccaaatt atgttggtaa
      301 caactagcta gttttaaatg agttagtaat taagaaacta gaagggactc aaatctcata
      361 gttttaagga aagctgttgc aatgcaattc ttgtatcaac attattgtag gaatctacag
      421 agcagtatat agagcttatt gaagtgaaac tgatacaatc accaaggtga aaagaaggca
      481 ggcagcagtt agcacaaatc cagtcacata catgctacat ctacatacat ggagtgaaaa
      541 tgcatcctgg cataaatagt ctctgactgt gcaatctgga tcacagagtt aatgaacaca
      601 cacataatga aaagtgtgaa aagaggtcgc agtgccatta ctgtacacaa tatgactcat
      661 tcagtgaaac cgttagttga gaagttaatc acttgacata aatagcctaa agagtaacaa
      721 tttacctgtc aaattttaca tgtaaagtca atcacaattg ttcccactga acatactgcc
      781 tgtactgtag cagaaagctg tttagcgggg atgttgcgcc gtatgagggt gataggagta
      841 atcaagaagg ggagggggct tctccatagg aagttcctgc aaacagagtg cacccacttt
      901 ttaaacccca tttgtcttgt taatgctccc ctctgattac ctgattgcac tggatgactg
      961 tggagaaatc tgattgcgta aaattatata gagcaaactt ttgattgggg agggatctgg
     1021 cgagcttgct gccaccattg acaggctttg tgaaatttgg cctcgctgat ttgaaatcag
     1081 ctaagtgtca cgtcccgtaa ttgtaatcag aacttaattt tttatccttg ttctctctca
     1141 cagcacaact ttttcttacg caaactttta aagcttgaac aatactgcag ctatgttcta
     1201 catacaataa cgaaaaatac actcatcctt ttcagagtga tctttcagtg agaaacactt
     1261 tgcttctgca tgggtcaagg acaatggata ctcattatgt tcagagtgtg cataactgta
     1321 tcttcaacac agcacataag gaattcatta gcttctcctc cactgttaaa acttatcgct
     1381 ttaaccatat ttcaactata gatgttcctg tctgttctca cttcattttg cactttgaag
     1441 catttataaa aaaaacaact gatcctcttc ttggcctttc tatcatctgg atcaaagctg
     1501 tttaaatgtc aggaatgaaa atgaatgtta aataaaggta atgctgtctt tattcatagc
     1561 cagcagttga atcatatctt tgtatagaat tttttaaata gattcaaagc ttaaaaaatg
     1621 atgtcaaaat gtaataaatc tttgggggac tcagtcctca aacatagact taatacaacg
     1681 aatttaagtc atggcatgtt gcgtcattca ttcttttctt ctctcacaag t
//
LOCUS       KCVB01000528            1383 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 24948pri ultra-conserved element locus
            uce-41, sequence.
ACCESSION   KCVB01000528
VERSION     KCVB01000528.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="24948pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1383
                     /note="ultra-conserved element locus uce-41"
BASE COUNT          458 a          253 c          315 g          357 t
ORIGIN      
        1 attttaagga atctcagtga ccaaccaacc aaatcttact ataccaacaa aaatcactcc
       61 agtttcttcc acaggtaaaa aaactgttgg tttatctatc tttttacatc tggcaagctt
      121 ttaataagtt attgaagcat acatttgtga aacagtataa aagtgtctga ttatccatta
      181 gtatacaact gatctactga tctaatggaa cagaagatgg tcagaaagga gaagagggat
      241 gaattagaga ttaaagctaa catgagagat ttttctagct tggtgcaggc agggagaaac
      301 agaaaggaga ggccttaatg tggtaagagt tagttcaatg gcatctgttt attcatgcat
      361 gtaagaaagt gtcttatgta cagatgtgtc acatgttttc cctctgctgc atgtgttagg
      421 aaactttgat gcgggtgaga ttagggctaa agagtgtaat gggaacaaag gcagctaggc
      481 agcgcgcagg ctggaaaaat gatcagacta cagggcgccg acggatatga gatgagctcc
      541 atgcgcactt acacagtcgt cctgccctcc agggcgcaca gtcagttcta gaccaatcag
      601 atcagagcag cagggcggag catgcaaggt gagcagagag agagcaatgg gagagaatgt
      661 tagcatcact ccatgagacg cagcctgcca aacagactta tgatctgctt cggccaactc
      721 tgctgtcttt gacaggcctt ctgtatgtgt acagctgtgt gcatgcgact gcgtgagtgt
      781 ggctgtgagt acatgtttgc atacatgtat ctatgtgtgt gcgtctgcgt gtgatggtgt
      841 cactgatgta tgcgcatgac aaagacagaa gagttagctt cggcactgga aagtctgacc
      901 agtgggctac atgagagaga aatgagcaca ctattcacat atcaaacact gcacaaacaa
      961 agagaaacaa accaacaaat agatgaacaa ataaaaggtg ggcacatgtc ccttgacttt
     1021 tttcggtttc tttggtactt atttttaaaa tgttgtggac taaaagaact attattcttg
     1081 ttggcaaaca tatgtattgt gcaagtggta gtcactgatt tgcaagtgct gcattatata
     1141 caagggaggt gtgttttcta atcaaaaaaa tacaaacttc aaaattttct gttctgtttt
     1201 agtgtggtta aagaaaccta cagtgtccaa taagcaagac agtcagggac catggccatc
     1261 ctcaacacaa tcactgaaaa acaaaacaaa gttatgcaaa ttacataaac agagaagaac
     1321 aaaaaaccaa aaagcacaac tgaaataaaa tgagaaccac aggttttaaa ccacccaaag
     1381 cta
//
LOCUS       KCVB01000529            1755 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25030pri ultra-conserved element locus
            uce-768, sequence.
ACCESSION   KCVB01000529
VERSION     KCVB01000529.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25030pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1755
                     /note="ultra-conserved element locus uce-768"
BASE COUNT          426 a          506 c          368 g          455 t
ORIGIN      
        1 ttttttcgct agcctcactg tggcattgtc aggctgaggt atgtggctct atggggtctg
       61 tggtccacac aaaggagcag ggaaagagag cgttgcggag aggccccttg gcagagctca
      121 cggcatggaa cactgtacaa ttagccagcc cctgcgttct gccggcttat aattatcagt
      181 gcttgcctta attgactccc ttagcctgct gcagagggac ccccccgcat acccgcttcc
      241 cccctgctcg acgcaggccg ccgagtgcca cgccgggctc tccaaagtca tttagaaaag
      301 cgctcatatt ttcgacacaa aaaaggggga acatttggtg agagagaatt catcatcagg
      361 tgtctagagg ttttccttaa tagaaaagct gagtcccact gagtgcgctc cgtgtagcgg
      421 ccagccacgc atgctcagga ccagcaagcc gccattcgca gcgggccaca agacattagc
      481 ataattttat ataacacagg cacccgcact ggaaatttaa tacatctcag caaatacgag
      541 acaggcgttt tgcataattt ttttctcatt ctccacctag cttcctctct cccttctatc
      601 tctctgtcct cgctccatgt ctctctcctg tcttctcttc gtcttggctc tcttgctctc
      661 tctcttgctg ccactctcct ttgcccccgc ccccctacac aaccacccct atctcctcgc
      721 cccatggtgc tgctctcact aggagagggg aagttcatta cctcagctaa atcagaatta
      781 gtcccttcat tttgacgggg cgaacttcat tagaaaggcc cttccttaat ccagcaggga
      841 gaactgatga atctacatgg gggcaataat ttgcttcaca cagcacattc agccacttcc
      901 tccacactgt ttcccccctt ccttttctgc gtctatctgg ctttatgtac ttatggttac
      961 atttttatta gatctacagc aaaaaacctg agaaaggaaa acacctgcat ataattaact
     1021 aatggcagca gaaagggtgt aaagaatgag ttatgctgtt tggaatgtcc cgtctcccca
     1081 acaggcttca gggcctctgg agcagccata gctgacttgg ccgacactca aacacaggct
     1141 tagtgagcac ggccgacagg agactcggtc tgaggtacta ttctagatga ttttacaatg
     1201 ctatgattat gttctaatgt tttaaagcaa acaaatcttt cctgtgcatg tggttacagt
     1261 aggtcgaatg tagaaaaaca gacttaagaa taagtaattt cctatcatta aagtaagaat
     1321 cctgcatcct ttagttttgg ggttttcttt ttctaaaacc cgcacaacac actgttttgt
     1381 aaagatctct aaagagacag agattcagca gttgtccaga tgcttagaac aaactcgaga
     1441 gcgaagagat gtgaatcagg acagacagcc cctgactgcc ataccacagc gcctggtgtc
     1501 aaacagatgt gcagcttggc agctacgctc cccagaaaac agagagccca cggattccag
     1561 ggtggcgctg agtcgtgctg ccctccctcc tttcaccact ttcccgtccc ccaacagtcc
     1621 caactccccc acactgtgcc cctgcagtct ccctccctca tcacctccac acaggggtcc
     1681 cgtgactggt aatctcgtcc ctcgaatgtg aaatctccaa cagcggtgcc ccgcgctttt
     1741 accccctacc tccgc
//
LOCUS       KCVB01000530            1520 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25069pri ultra-conserved element locus
            uce-1077, sequence.
ACCESSION   KCVB01000530
VERSION     KCVB01000530.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25069pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1520
                     /note="ultra-conserved element locus uce-1077"
BASE COUNT          436 a          362 c          284 g          438 t
ORIGIN      
        1 ctaagtgtgg aaacaaagtt cgatttgaaa atacgttatt gtttgcgcta atcaatgcat
       61 attaaaaagt agtacctatt ctattgaaaa agcagcatat gaaaatctgg attcttcagg
      121 tgaaattttc aggttgtccc aaacaaattt tgaactatca gattagacca ccaaaatcag
      181 aacacatgct gggttagcat agccaataaa aacaaacttt tgcttaagaa actataaaaa
      241 gaaatttgca attttccaaa cacttggatt gaacctcagc ttatgacact atacttaaat
      301 ggtttttttt ctggctcttt ttcgattcat caaacatttc acataggaca gtttgctgca
      361 caaacattca gcccggtcct acttatttta gttaagcctt tattgacaag gttcactgat
      421 atacagtttt attttgtatt atatttgacc aaggtctggg accttagacc cattgtatag
      481 ccaagccttc tcttagacag ctgtggcttg gctggctctc agctccccct gtcttaacct
      541 gaagaattat gtgctcagat aattagctct tataggtaca ctctccattt ctccactcca
      601 ctgggcacat catcttcctc gcctctttat tgtttggttt gctttgtcag atcgatgtct
      661 tgctactgcg taaccagcac tcatatctca ggctgcataa atcacaacaa ggtttctgtc
      721 gctcgggagg gtggtgtggc tttgctgcac acatgcacac tcacaaacac acacaaacac
      781 acacaaacac acgcacacac acatacacac ataaacaagt gggtgggcca ctttatgctc
      841 cacatccaac aaactctctt tacctctctc cttgttactc ctgtccctct gctctcttct
      901 tctcttcttt cctccctgca tctccactac cgctgagtga ccactgaaga ggaacccatc
      961 agattatcct ctacccggta gtgatgggta gggtgggtgc ggcagaatgg agggagggta
     1021 ggggtctcta tcacgcctta tgattgcagc gccacccttg taatgaattc gaaaaatgca
     1081 aatggctctc cccatgtaaa agttgacatt gtctcctgtg ataaattttc tgctgaggta
     1141 agagacaatt tagatgcgaa ttacaagcag gctctttgtc actcctcagt tccatttaca
     1201 tccttaatta gctgattccc tcccgctccc attagagggt gagagagggg atgagagaga
     1261 aagacagaca gacaaaaaga gcgacaggga gaacgacata gaaaactgac gtcttgtgct
     1321 gagctctgtt acacaaaagc gagctttcaa atattataaa cacacccacg tctattcatc
     1381 ctcacacaaa ggtacataag ccttgattat ggtgcaggaa atgagccaga aacaccatcc
     1441 catgtgttag gacctcaggg ttgatcagca tccctgggtc tgtgtctctg ttaatgtcat
     1501 ccttaacagc aaactaaaca
//
LOCUS       KCVB01000531             944 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25104pri ultra-conserved element locus
            uce-922, sequence.
ACCESSION   KCVB01000531
VERSION     KCVB01000531.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 944)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25104pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>944
                     /note="ultra-conserved element locus uce-922"
BASE COUNT          261 a          202 c          226 g          255 t
ORIGIN      
        1 ttttttttgc cctctgtcct aggtcgcagc tgacagggtg gagggagggc cgggtgggca
       61 gaacaatggg agtgcggtgc agggattgtg ggagagaatc taggctggag ccacagtgtc
      121 tggagcgccc ctccactccc ctcatccagc ttttgttcag ctgcaaaagc tatcagcact
      181 tcctactgac accaacaata actccacaca gctgggccct ttttagcctg tttacagcac
      241 atcaaggcaa ggggagagag aagacagagg gaagaggcag aggaggggaa agaaaagtga
      301 aacatattaa ctcctgattt taactgacag aatggcagtg ccctctctca gctccccttc
      361 cctccttcat cgcacacaca gaattcattt cccagtgggc tctttacatg tgagccgatt
      421 atcacttagt attttatttc tacaacaggc tactgtttgt gtcagttctg ttgcgacatc
      481 ctgcatccat caaacagcag aaaggcaact gttgtccttt ttttcaggac attgtgtatc
      541 ttggctatat ttgtaccagc acacaaatca tttcctagta gtgttttttc aggataaatt
      601 gttccatttg gtggagggga aacgtatttg cgaatacgcc ctctttatcg caacagcaaa
      661 tagatctaca agagcccact acaggaagcc gcaagagaaa aagttggtac aggaattcat
      721 tcagaggaaa taggacattc cagcaatgct ttcacacttt tgctgctgtt attattactg
      781 tggggacaat aagtagtacg tctaatactt cctgaaacac gaaggtgacc gaaccacaat
      841 aatggcggtg gagaataaat ggtttcttgg atagaggatt gttcttcagt atgattattg
      901 aggtgcaccg ttgaaaagaa atgtgcagga atttgattat gaga
//
LOCUS       KCVB01000532            1511 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25145pri ultra-conserved element locus
            uce-161, sequence.
ACCESSION   KCVB01000532
VERSION     KCVB01000532.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25145pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1511
                     /note="ultra-conserved element locus uce-161"
BASE COUNT          440 a          330 c          286 g          455 t
ORIGIN      
        1 ctatttaatg taaaaaacaa acaaacattt aaataaaaaa aaacatttga ccagtcgact
       61 acatggaaca aacataaccg tgtcctttta atcctcttga attcttaagc aaataccatc
      121 taataaagaa aatgaaatac aattttagta tttattgtga tataaatttt gcccaaattg
      181 cccacccatt ggttactgtt tgtttactga cagtttactg attttcgctc caaaaaacag
      241 caagtaaacc ctgaattttt ttttactgaa gatcttttaa gttataacag caaatatcaa
      301 atataactaa gaagcagaaa taagccaaca tgaacatgca gtattgattc actggccctt
      361 ttctcttttt ggccacttac actgctaagg ctagcatttg aaaaatactt accttgttat
      421 ggaaagttta atgtcatatg caattgtttg acccttatga atattaaaga atcccatctc
      481 tggcctatta aaaatgcatg tggcattgtg gttgttgatc acgtttagaa cgcccatgca
      541 aattggactg tcttccctgt caaggcctaa gatgaataat gtagttgtgt tgtatattca
      601 gggacacacc attgacagtc aattaacaag tttgattggt gtgtgaactc agtcttttga
      661 actgttaatt ttatgtaaat agtattcgct ctcccttctc cactgtctct cccccatcca
      721 ggttcaccac agaaagatgt gcccccacca ccacaaaccc ccaaatcccc aattccacct
      781 cccactaaga gtaaccagag tgggatcctt aataattgat catcacacac ctgcttggta
      841 gaagctatgg gagtctgcct ttaacagtca agggggccca tgctagcctc ttttgatatt
      901 caaatagaca aatggtaacc agcctttgtg aattcaaaat ggagcgagct cttttcatgg
      961 ggacgagctt gttaagaaat tctggccacc cgtccaacct gatggagccc ttctttgttg
     1021 gagtggatgt gatggcattt gatggctcat aaatggaatg ccaatgacaa tttgttgttg
     1081 atgtgtggga atctggcttt tatgtgaggc agacatagta atgccggcgc actggcagat
     1141 tgtgcttgca ggtaaatgat ccggtgattg atccaactgg ttgaaagcag cggagggcct
     1201 tatctcctcc ctttcttccc cctctctcac tcacgcccat cttcgctgca tctcttgtct
     1261 cccgcactgt cccagtgatt tggagcagca gagatggaag ctgaagattc atcaaagttg
     1321 acaagtgaac ccaggagctg gggatcatgg aaaacccatc tgattatgga aatagccatg
     1381 accatgtctg ccctctagct aaacagttgt gtggctagcg tcttattctg ctagtatccc
     1441 ataaaagaca tacctttcag caaggggcaa atattccgtt ttaagaattc cccctttgaa
     1501 caagttgtgt t
//
LOCUS       KCVB01000533            1503 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25187pri ultra-conserved element locus
            uce-124, sequence.
ACCESSION   KCVB01000533
VERSION     KCVB01000533.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25187pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1503
                     /note="ultra-conserved element locus uce-124"
BASE COUNT          430 a          283 c          328 g          462 t
ORIGIN      
        1 agataggcat atccctgctc atcttgggaa cttgtttcca tagttacatc aatgttgttg
       61 aagtgagaag atcaggtatt ccagtgagtg atcctttaca tgagctttgc tcctctccta
      121 ttgattttca tcccacgttg aaaaaataca gcccactgca aactgtctca gagatattgt
      181 ggaagtaatg aaagggcaaa atgtgtcgga aatgtgctgg acaagaaaag aagagaaaat
      241 aaaagcctct gaaaaggcgt tgagagattt tgagatgtct tgtccagctc ctctgggggt
      301 caggtaacgc cgtcagagcc gcaaaccaca aagcatgaca gcggtttgga ataaaaagct
      361 aatcgctgtc tctaacagtc gatgatcgcc atgttgttag ccacatgctt atgtgttcat
      421 aagaaatctc cacaacagcg tgctgctcgc tgtaatgcta atggctctga aaagggagtt
      481 aatcactgct gcgtggttag ccacatgctt gcctcttgca gcatggttgg tgtgtttcag
      541 aggttagcaa aacactggcg taacacatca tcgctcttta tgaggctggg gcctgcaaga
      601 gagagagggg gcccacagac gatgctaaaa agacggtaat gacagccatt caccgcaatc
      661 caattatgca aaagggagtc taaacaattc catcatcccc taaattagat tgtcctaacg
      721 agataaaggc accaattaat cctgaattaa tttctacagc tcattttccc cagaaaacac
      781 tctaatatgc aaatgccaat gagagacaga gaggcatcct tctgcagggg cttacaccac
      841 ttctgcttgt tggcttttct gtttgagtga acttagacac ataagcataa aaattaagga
      901 atataacaca ttaaaaagct caggtgcact gaagagaaat tgtttaagag taaggacaaa
      961 tttcactgga tttaacgttt agagttggat gaatggcatc gttttattga cactagatag
     1021 tgcagttcaa taagctattt atagtgctgc tctagtgtaa cagaaagcaa atagaagcca
     1081 aaacaaagtc aatacaacta atttggaatc tggatttagt tgattaaagg taaggagctc
     1141 aaaagatttg aatgtttcag gtatgattgg gaaatatcga tggtgtctgt gctttgaaat
     1201 aaacatttct ttgatcttat ttgatttgct ttgtttttat gctgcaacct tcacatcgtc
     1261 ggtttgtgat tatttgtatt tttgtggtca ttgcgtgtct ctacagtcgt tttgcgtctc
     1321 cttttgatca atttgtggtc ctttcgtatc ttttttgagg tcagtttgtg tctctttgcg
     1381 gtctttttgc atctctttgt attcgactga ttgactttct aataagatcc gattgacttt
     1441 ccaaaattgg cccttcggcc ctggggccaa ttttgataag tttagtaatc catctgtggt
     1501 gac
//
LOCUS       KCVB01000534            1681 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25227pri ultra-conserved element locus
            uce-1116, sequence.
ACCESSION   KCVB01000534
VERSION     KCVB01000534.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25227pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1681
                     /note="ultra-conserved element locus uce-1116"
BASE COUNT          457 a          346 c          448 g          430 t
ORIGIN      
        1 ttcctgccag tcacgccgcc tcattagaga cagagagaaa agggagatgg agtattcaga
       61 gaaagatggt gagagaggga ttctatctgt gatatttaca ttcgtgagaa accaattttt
      121 tagtaggatt acaatcacag gacggctcca caactgtgaa agaacccagt tcgcagattc
      181 ttaaattagt atactgtgtg tttagcacac tagcatagca atgaatagat gctgcagagg
      241 caacttgcat ggattgacac acaggcttga agtagaggag agagggaggc ccgttggggt
      301 gtgggggggg gagataacgt gaaggcatga aatattaacc cctggctaaa cacccgagct
      361 tagccgtctg tgaaatccca ctgtggacaa tgctaataca tgcattcaca agggagatga
      421 tagggccttg ttgcaccgcc agatgggaag tgtgcattaa gaagctctct catgtattca
      481 tacgcccata ataggatcta taagacgaaa atcccaacag tcatttcagt aacgccgacg
      541 gaacaatact gcaaatttca gaaacacgtg catgcacgta cacatgcaca ctgtctctct
      601 ttttcttaca cacacacaca cagacactca acatcctttg cctctgaagc taaatagttg
      661 attgatggtc taagctgtga agaaatgctc tcctttatgt ctctaaaggt ttgtaattgc
      721 tgcagtgaaa ggatgctcac tcgcctggaa tattgatcct aatgggatta ctatcctccg
      781 ccagagagag ggtgatggag ggaaagagat gaagggagga tgagagggag ttgagggggt
      841 ccgggcagag aggtgagcag atagacagag ggagaaatct agtttgtcct tggcgttttt
      901 cattgatcat tttttttgca ccgccgtttg gtaacgactt ccgtacggga acgatataga
      961 atcgctctgg aaaccagaga tggagaaaat actgggaaca gcaatgaagg gggggttaag
     1021 agttaggctg tctctcaagg gagagatcac ggaggggcga tacagccctc ctccctcctg
     1081 gtcctccggg acggttgttg tttttcgtcg atgttgccgc agcggtacgg gtgctgctta
     1141 tgtcccggca gctgcaaaca aaagcgggct caagtggaat gcggaagaga taaaggattg
     1201 ttttcaaaaa aaaaaggaat cagacttata taactgaaga gtcggaggtg tgatgccaac
     1261 agaaactgaa atagagaact accatatgct ctctgtgacc atattgtaat tctggggaaa
     1321 gtacagctca ctgcctctcc tgattattct catcactgtg ttctgatttc accccgccct
     1381 cttcatctct cagaattaat cagcatggag ggctatcagc ctgtccgtca ttctgtccgt
     1441 ccatccgcgt gcctgtttga ctgcagtaac cccagatggg aggcatggcc agactgtgtg
     1501 tggccagact gtcctctgca gctaattgaa acgttacgat gaggccgagt cacggaatct
     1561 gcatatgtgt gtgagcctca gctgatagcc catgtgtgtg tgagtatgtg tgtgtgtgtg
     1621 tgtaagtata gttgtgtgtg tgtcagtctg tttcagcctg accataatgt tggtcatctg
     1681 c
//
LOCUS       KCVB01000535            1769 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25289pri ultra-conserved element locus
            uce-225, sequence.
ACCESSION   KCVB01000535
VERSION     KCVB01000535.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25289pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1769
                     /note="ultra-conserved element locus uce-225"
BASE COUNT          548 a          371 c          306 g          544 t
ORIGIN      
        1 ttcttatgta tttaatagct atcacaggga atgttaaaag gcattgcatt ttagaggtat
       61 actattacgg tctttaattc taaataatgt ataggctgca gaatgtgtgg ccaacactga
      121 catgaaatcc ataagatgtg atgttgatga tgattttaga ccacagtgaa tgtttaataa
      181 tttaggctga attactataa ttgaattttc tgcaggtaac cagcagaaca cacttatctc
      241 agaccctctg gatacaacga tttgtctgtg attgctcagt tcaagttctc attgcaagtc
      301 cacagttgcc aaactgcatt ttaaaacatt tgcatgagat gccactgagg acgttgcctt
      361 ggtcttaagt tttattttaa ccacaaactt tttgacctgg aaaaaaaaaa tttattacat
      421 tattattaca tcattagttt tataggagat taaattatgg gtagttacat ttggtgcatt
      481 cagggagaga actgcataat gagtgtgcca cgaatcaaat agcaaaaagc cactttctgt
      541 tacgactaag gccatgtatt atgataacaa aggacaagtg aatgtaagtg cacagaggtc
      601 attttttgaa ggcaagagaa ataaacggtg gggttttttt ttccgttcca aaaattttct
      661 tcaaagcatg caacaatgaa gggaacaggg gacaagagga aaaaaaatct tcattgaata
      721 ttcattaacc ggtgcctagg tgtatacatg gctcttaact actcttgttt atgacttaag
      781 gacttgtagt actgacctta gtttaacaca gcagcagata ccaaattaac ctctagcctc
      841 cacagggcta gccatgccct gctaacaagc aacctgtcta gacacacaag aagtacttta
      901 tgagtctacc tctcacaatc cctctcgctc tctctctctc tctctgtctc tctccctctc
      961 tctctctttc cctcaacctc tctgtctctg gtaacacgta ctggcacatc ctccctccct
     1021 ctttcccttc tatctttcac ctcaccattt catccccatt tcccccctac accatttttc
     1081 atgtacctcc caagctgtgc agtgtttggg tcatgcaccc agcaaccaga gttttatcag
     1141 atggtagact ttctcttatc cttcacaaca tagtctgcta caacagagtc agaaaaaaat
     1201 gaaaggatca gaaacctcta ggaagcttca tttcagttac actcacaaaa acaaaccata
     1261 ataaagctat gatgcattta aaaatgttac tacacttctt tttgtttaac attttttttt
     1321 ggcatttctg ctatatttgt gtgtagtgtg atatagatgg tgttacagaa atgttttaat
     1381 cttccatcag aaaactggag aacttctgct ggaactgtgg ccctctcaaa tttcggaccc
     1441 gaaaaggggc caagctaact taaaggatga cactaatgga aagctagatt acaagtgcca
     1501 ttattaaagt caacaaaact ccacatatcg taaaacaatc gaactccaga ggtgatctgg
     1561 aagctgtcat cagcaacctg gtctctcaca aagacaccaa gggtcaaaga ggaaactgat
     1621 ccaccatgca agatttaaat gtagcttaat tcatagtaat gttttaataa ctgcctgaaa
     1681 agcactgagg cttttgtgta actaaactgt atgtaatgaa catttagcag atgatcgcta
     1741 agaaaacttc ttttttcaga cacagtgtt
//
LOCUS       KCVB01000536            1628 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25334pri ultra-conserved element locus
            uce-1252, sequence.
ACCESSION   KCVB01000536
VERSION     KCVB01000536.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25334pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1628
                     /note="ultra-conserved element locus uce-1252"
BASE COUNT          437 a          351 c          286 g          554 t
ORIGIN      
        1 aggactaaaa tcatagacca acgcatgtta aaatcacccc tcctacccca gagaaataaa
       61 tccagtccaa atggagctaa agacttgaca aaccttcccc agagccacaa tagacattac
      121 aaggttttac agctttagaa gccccatgac taatagactg atcctataaa atcagtggag
      181 tgccccttga aaacatgttt aacttttact ccaaaaaaac acttttatta tttcaccagt
      241 aatgtcacca ttttattctt tttaaccatt ccaggacctt catagtctct tcccctttaa
      301 aaaagacaca ggcatctttt gcacataaat ctgacttgag gtttacatca tcgggtaggt
      361 gtatgtgttt gccaatgtcc ctttcaaaag taagccctct gtttgatctg cagttataca
      421 gagtgacagt acttaacgtg tagctctcga ctccttaagt acttgtccag aatccagaat
      481 aaataaactg cagtggagtg atgaggatcg taaattctct cttcttccct ctgtctttct
      541 ctgtctctct gtctccctct ttctctcttt aatatgcctt aacatctgtt gtcaagttca
      601 catatcctca catgtactca cacaccaccg cccacaaact ctcatgttgg tataggttgt
      661 tgtttttgag ttgtgttttc ttcttcatgc tttgcctgta cccttcttga gtaatgcagc
      721 tgctaggtca gggtctctcc tccagccttg ttgctagtct ttatgttatt ctggtttgtc
      781 ccttcttacc ctgctgtctt ctagctggac tcaagggtaa gtgcttccct gtagccggca
      841 cagctcagat aatccccgct gattatctga caaatgcaac gttctttctc tcttctttcc
      901 ctcttctcct ttttcacgtc tcgcctcatg tcttagatat tagttaagaa gttttttgtt
      961 tttgatgacc tcaatttgag gatctaagta taagcatcca ttcgtgccag tataggatgc
     1021 atgtatttta ttctaagacc ttattatttg actcagtaat gaataaagtt tatattatag
     1081 ttattactgc ataattgcct tctctcttta ttcatattgg tgactagacc cctcagagta
     1141 aagcaatatg atgaaaaaaa tgtgacatat tattgaaatg caagtgcaaa tttctgaaga
     1201 ttgttttaaa gcagttagat gagacctaga aaatatccag tgtttgtaaa aaatagttca
     1261 aaaatagaag tgtggaagaa gtgtgaatga tgacggaagt ctgtgatgga gttatttttc
     1321 caaatgtatt ttataaatga gtgaaatttg tttttaagaa ttcctaagtg ttgatgatgc
     1381 tttgctgcag agggctcaaa cgcttttagt tctttttctt tgaacttttc tggatgagag
     1441 ttggcaaata tgcacgcgga gtattctgaa tgccaaagac aaggagtagc taatgccgcc
     1501 ccttcttacg ataaaatact ccggcccccc cctcccttcc tcctactgtt cagtagatgg
     1561 gcctgtagat agaggctaga ccgttgttac acctccagca aggagagatc tcatgttgat
     1621 tttttttt
//
LOCUS       KCVB01000537            1323 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25374pri ultra-conserved element locus
            uce-508, sequence.
ACCESSION   KCVB01000537
VERSION     KCVB01000537.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25374pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1323
                     /note="ultra-conserved element locus uce-508"
BASE COUNT          362 a          291 c          293 g          377 t
ORIGIN      
        1 cccagctgca aaactctgcc aaaatcagct ggttatggtt cgttcagcag gtaaataatc
       61 agtggtgtgt catagctgag tgtgttctgc tgatgtggac ttagatggca gtgtaatgta
      121 gtctagatga gacgtcctgt tccattcaca ttaaataaat tcattaacaa gaacaccctt
      181 gcaagtaccg actatagata aagcattttg atgtacaaaa cattcaaaat aggggtagaa
      241 atgtaagggc aagggaatga cccccaattc tttccgcttt ttgtcaaaga caaaggtaga
      301 ctgtgattgt gtgtgtgtgt gtgtgtgtgt tctctccagc tgcctccgtt gattacggtt
      361 cctttgcaga cagatgcagc acctggctag agctgcttag gctgaaagct cacaccataa
      421 gacgaggatc agttaagaca agtaggagga cacagtctct agaacactct ggtgatcata
      481 cccacaaata cagaacacag tctcacaagc agtcccgcat acacacacat acacacatgc
      541 tcgcatagac tcacacaccc ctccatgcat gccgctgggg ggcggagcat cctaaatcaa
      601 gcaccagctt tttagctaga gtgtgaaagt tgaagccccg cccctcagag ggatccacaa
      661 tcaacagctt ggctttaaaa tgcctgtagc catggtaacc atgatgataa attaataggg
      721 aggtggggtt attttggggg tgtttttgtg catacagcgt gcttgtactt gcagcaattt
      781 gatgacaatt actggaagca tttctttgat ttttctctgc atttgcattc atggatgttc
      841 ctaagctgag aaaacaatac atgatttgag gatatactgt acacatacaa atctcaatgt
      901 ggtaagagat caccgtgggg cagtggtgca atgcttttct gcaagatctg gtatgatttt
      961 cagtcctgtg cactgtaagt gaactacagt ctaatgtgtg gctctgaagg tccctggttt
     1021 gatggatgta agccacgtca ggtaaatttg caaaaagaca atggagcatc tttcacactc
     1081 tggctcgtct acttgtgccc agctcagctc acagctgtct ctgtctgtct caatttctca
     1141 aacttctttt acaaatgcaa acagatataa acatcatctc atgtacttca ctgtattgca
     1201 aatactgtat tcacacatgt aagcatacac agctgtgctc aggtctgtct gtctgcagac
     1261 actgtgtaac tgtcttgtct gtttgcctca actgcatgtc atgcatggca gcgcatgctt
     1321 tcc
//
LOCUS       KCVB01000538            1737 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25412pri ultra-conserved element locus
            uce-338, sequence.
ACCESSION   KCVB01000538
VERSION     KCVB01000538.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25412pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1737
                     /note="ultra-conserved element locus uce-338"
BASE COUNT          627 a          259 c          424 g          427 t
ORIGIN      
        1 gatgcaggaa gtgagaacag gggaggtaga tgaagggtga aatgcaagag aaaggaggcc
       61 gtaagagata aggaagacag gtggggtgtg aatagaggtg agaaaaaaga gtggtatggt
      121 aataaacaag gcaagtgatg agacagatgg aaggaaggag gaagaagagg gcaaaagaca
      181 gaggaggagg aaagagaata aaggagagga tagagtggaa gtaacaatgg tggaggtgga
      241 agagtcagac atgaattaag tttacatgga gacaaaacac aagagcagac agaggtgata
      301 taagcagtaa agaattgcga agaggcaatg aaagtaacag aaaatgtgta tgatgaaggt
      361 acgaggaaca tatgaagaga aaggggaagg gataaagagg gaagagattg gtagcaagag
      421 tggtttaagt tatcaggtgg gagaagtgaa tggtgggatg ataatggggt gcatccatgt
      481 agggagaaga cagcggagga aaggaaggca atgggagaaa acagagaatg aaggcaaggt
      541 gagatgcaaa aagtaaatga aaaaaaggaa caaagagagg tcaaagagat gagacaggag
      601 agggacagga gtgatcatgt aatcaggtga acgctcacaa cgtcaagaac tcattcaaat
      661 aacttcaatg tgaggttaag ctaacttaac caactaagcg agcattataa catggttatg
      721 ttcagggtca aatcattgtc tggtgttata aaagtatttc aattaacagt caaagtgata
      781 atcgcttccg cgtgtctttt gacataacac ataccactat accatgtgta tttcaagaca
      841 attccaacaa taaggttgtc tctacctgtc cttgtacagt ggggacggga gaccaaaagt
      901 atgatgagtt gaaatgggaa tcgtccatta tttctgtaat taaaaagcaa cacacttaga
      961 aaacacctgt ccttctcctc ctcttcctcc atcttactat actctccttt ttttaaactt
     1021 tcatttgaaa cgagaccatg catggctacg tttcaatcat cttagtttgt ttcctttcca
     1081 tgtatcctct cctccatcag cagacacact aaacatgtca tgtttcagga tcagaatgca
     1141 gtactaacac gtcagcagct gctaaatttt agttgtggct agttaatctg tcttctaaat
     1201 caaccacttt gctaggcgag caccgatcgt taagagaaca ctaaaaaagt gtaaaatcgt
     1261 aacaaaaccg accagttaca gggaaatgat tttagattta tgctgttagg gtaaggttgt
     1321 ttttttttaa agaatatttg atgagattta cagagaggag aatgtgaaag gaatcaggta
     1381 aagatgttta atcccagaag ggatggcatt aaaaaaaacc tacaggagga ctgaagaggc
     1441 aagagttgag aaaacactaa taattatcaa attatcaatc tttgaggcct ttgataattt
     1501 tttttaacag cccatgcatt tcctcactag tcccttaccc attatgtgtt gctattaata
     1561 agttacacaa tctctaaacg ttactttatc agcagcatgt gaataagtct agttttggaa
     1621 aacaaaataa tgctgcgtca aaagcattta tttccacagg aaagtaacac agtggcgtgt
     1681 tctcctaaac tagctgctac tacagtatgt gcatatgatt tgctgaagac aacactc
//
LOCUS       KCVB01000539            1602 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25494pri ultra-conserved element locus
            uce-450, sequence.
ACCESSION   KCVB01000539
VERSION     KCVB01000539.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25494pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1602
                     /note="ultra-conserved element locus uce-450"
BASE COUNT          425 a          349 c          340 g          488 t
ORIGIN      
        1 gtttattagt aatctcaaaa catctccatg agtctatttt aatattttaa ttcaagagag
       61 tagctaaaaa aataatgcta gctaatatga ggcacacagt atgttaagct agcaggatgt
      121 tttgtaattt gtccatgtga tttctcctgg ctccaactct atattgaact gtgacatata
      181 aaagtggtta cagtcttctg cttcatgaga aagagaataa gcatatttcc taaaatgtca
      241 gttccttaac ttaatagcac tcttgtgttt acctggtgta aattgagaac attgtggatt
      301 atatttgtat tgtgagtaca caccggcccc tcaatgaaag cctattttct gcctgtttaa
      361 tttgttctgg agctgatgcc gttctcccac atttacagaa ttcaaaatga aaaaatgcca
      421 cttgttcatg tccgtacaga taaactcaac cacaacaaat tcaaatgttt tgttcacacc
      481 tgttattcta taatttacca ccagtttctc ttgcactaca ataaagagac aggaatgttt
      541 ttgtcattta tttattcttc tccagtaatc gccttgctaa cgcccacatg ggcctccata
      601 tggagtgatt gcccacggtt gtgtgcggcg tgatgccagt aggtggtcct gcccatggct
      661 tggtgggtga gcatggtgta gtctttgctg ccactgccgc tgacatatca gagctgttta
      721 ttaagaggat gacaactcta tcaagcccca taagcgggcc ctcgtgcaca ggagttcttt
      781 tgttactttc agggagggtg tcatcaatct tgctgccatg ctactctctt gagctgttga
      841 tggcaaacac gattcctcac tttctgcctg gcctctcccg cctcgcagtc tggaatcagg
      901 cgtggtgagg gggatcgggg agataggagg actttccccg agagtgcctt ttcttgacag
      961 gcgacagagc ggaccgtttg gacaccaggg gtggatatat gggaccatgt aatgaaaaga
     1021 cgttgacagg acctgcttgc tctgtaatga aggtcctctg gagctgtgaa tggatacaca
     1081 catctttcac gcttttgtct ccctgacttt cccacagaat ctatctcatc catttcaaca
     1141 aaaaaaagaa gaaggtgtaa gagaagcatc ccagaaaacg gctcacagtg tcaaacattt
     1201 acatgcaacg acgtggtgtg aatttttaat gaaacagcag ctaagttgat ttacacccac
     1261 gatttctagc taaatccctt ctgcatcagc ccatgaaggt gcagttgaat gttcagcctc
     1321 tttcctctct ctgtcagtgc tactcaagac tcagtgtagc tcacagagat ggactatgat
     1381 gtatgtactc cactttagca ttggctgagg tcacagactc cataaatgtc ttcgacaagt
     1441 ctgatatttt aatctctttt tagtgagggg atgttgggcc tctgcccaat caatacacag
     1501 ggcaatagcc ttgccaagat atctcatggt agcttttcat tgttctgtct ggactctgaa
     1561 atgtcagcca gaatagcatt gctcaaactg aagttgaaat gt
//
LOCUS       KCVB01000540            1494 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25537pri ultra-conserved element locus
            uce-85, sequence.
ACCESSION   KCVB01000540
VERSION     KCVB01000540.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25537pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1494
                     /note="ultra-conserved element locus uce-85"
BASE COUNT          353 a          376 c          395 g          370 t
ORIGIN      
        1 gatacagcgt gccgcgaccc cgacagcctc gcttttttgt tacttcacct ttccccggac
       61 gcgttttgct ttctccggtg cagcccgggc gctcgtgcag ggagctccga attcttccag
      121 cactagctga ccggggcgcg agctgccagc cacgcgcgcg gcaaacacct gagcaggtcc
      181 cgcgcggtat tgaggaaaag tgacagcggc accgtccgag tatgaggtgg tggtgccaca
      241 gcggtacgtg acggtccagg tgatgagaag ccaccgagaa tgttgtggga aattgctttc
      301 aaaatgaaac gtggcttctt tctcccgcga acctcgcgtc ttcgtcgtgg ctctttttag
      361 tttatcgaaa ctatttcagt ctgttttgtt tttcttttcc ctccaggaaa gcgacaccga
      421 cccaaaccga ccagcctcct gtgttccgtc tcgctattct acactaggca gtggtggtgg
      481 tggtctggtg gcatggacat tgcgctcttc ccctgcatct ccaccctgag gggaatgctg
      541 cttttactac ccacgctttc tttatgttaa tatttctcaa tgccctaccg gcggtattaa
      601 tagcagccta ataattctac cagtctaacc cttcgctagc ctctgtctcc ctgctttttc
      661 tttgtcctga ctctggctac cagtctccct cctcctcctc tgcctgcctc tgctggcggg
      721 gctgcggcgc agacacacat aataaagcca aggcaggcag tgcttggctg gaaatgtagc
      781 cgcgtcccag cagggggatg tgagagaagg gtcccggcgg gggagcgctc tgatcccgca
      841 gggagcagct cggcacagcc cgggttggct gaggcagaga ggcaggagag aggcaggctg
      901 ggctgacgga gagaggggag gcagagggga cagttaggca acagcgccta gctgtggcca
      961 accggctcaa ctacaacggg gactctgaga atacagagga aggagacaga gcgaaaatat
     1021 ggacttggat tcaagttgga tgtggaagag gcctctaaag ttaatggttc aataagtaag
     1081 tttgacaaac aagacgaggg tcattgaaca gcacaaatga tcgaaaggtt atgtcacctg
     1141 gttcgaaacg agttttaatc acagttcagc tgtggaggat ggtgctatca gccagcagcc
     1201 agcgtaacaa catgtcaccc tgtagtccca gtagcaatga cagtgttttg tatctttgcc
     1261 taaaacattt ttgctgcatt taacagcatg ttcctttctt gtgtggattc actagtcagg
     1321 ccaattactg gccactgcaa gctgtgtcat aaagtctttt catactcctt ttgcccatat
     1381 gtgtaagaaa atcagaaatt cccccaaaaa aatacaaata gcaatgtaag aaaatattac
     1441 gttaggagtg gattgaggaa aaacttaatt catttactga aatgccacag tgta
//
LOCUS       KCVB01000541            1472 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25583pri ultra-conserved element locus
            uce-78, sequence.
ACCESSION   KCVB01000541
VERSION     KCVB01000541.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25583pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1472
                     /note="ultra-conserved element locus uce-78"
BASE COUNT          407 a          340 c          308 g          417 t
ORIGIN      
        1 gatgcctgtg gtgactacag gctacagtca gccccctaac gaagggagct gtgacacagc
       61 cacaaggggt cccatttaag ccaggcgcac tctgaagtgc actgtaagca ccctcctttt
      121 ttgcttgtgg ctaagtaaat tctaaggaat gctatgaatt tagcaaccag agatactgat
      181 gtctatagag ttctgtagtt gaagttaaaa aacaagtccg gcatcaaagc ttcagacatg
      241 ctgccagtaa ttgaccatgt aattgcttag gtaccagagg aatcccttta aaataattca
      301 cacgttttat ttctccattt ttttacattt tcgtggcatt ttagcatcta tcagtgtcag
      361 cagccgtgtt attattatgt ctgtattatt ttgtggttgt atttgcacca tgtgcaccac
      421 ttccgtatcg tgctggctcc gagataaaaa ggaattagat taaaagaggc tgagacgatc
      481 tcatcatccg cactgttaat ctctgtgaaa cctttaattt tggcctaata tctgcctcca
      541 tttcagaaag agagtacaag tgcacgagct gcatgctcgt gcgtgcgtgc gtgtgtgtgt
      601 gtgtgtgtgt gagagagaga gcatgtggcc atgcatgttg gtgtgtgcac atgtgtatgt
      661 ccatatgtgt gtgggtgaag tggatgatat tgagtctgac ttcataaatc acccagacac
      721 tgataaactc cccatgcacc cacacaccgc taatgggcaa cccttaagga gtgtgagtgt
      781 ctcgtgcggc gtttgattca acatttatta accccctcac acacactctc tctcacacat
      841 acgacacaca ctcatttgag ccccagaaag agcgaggata tggttggtgg cattcacatg
      901 ttagtgcggg gttttgacct agcagtgctg tattaatcat ggccaataga agtaagatac
      961 tttttagctt atacagccac aacaaaaacc tctgcatttc ctaacattac tttccttctc
     1021 ataacttata tcctgtctcc aactccccac ttcccatctc atatcctggc acccctcctc
     1081 attatagaac aattttctgc ccccacatca aataaaaaca cactctgtgc ttccatctct
     1141 tgctcttcat acatgtcggc tcataattct aatgttatca caacctgcac ccctcggtac
     1201 gagtcgcagg cttagcggct gggaccctgc agaacttaat cctgcagcca catcagtgat
     1261 tcataaatag ctgcttttat tcttcttaag aagtttgtgc acttaaaaaa aaaaaagaaa
     1321 aaaacaagag tgaaactttt tggaagatat taactcagct gcaagaggtg cactgctgct
     1381 gggaagccag aggagtatcc ggcatgcaga taggcagtgt ccagcagtca gtcagacaga
     1441 tagacacagg gatagatagc tggatagaca ga
//
LOCUS       KCVB01000542            1447 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25628pri ultra-conserved element locus
            uce-714, sequence.
ACCESSION   KCVB01000542
VERSION     KCVB01000542.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25628pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1447
                     /note="ultra-conserved element locus uce-714"
BASE COUNT          382 a          300 c          282 g          483 t
ORIGIN      
        1 tctcatttgc gtcctgcttt cagctcaaac ttttaattct ctttatatac tgaaatatta
       61 aaagcctaat atgtcacctg tcatgagcgt catttatcat cgtggctgcg gcgtgtcctg
      121 tgggcctgta aagatttcct tccaattact ttttattgaa ttgccagcat ttgttgccgt
      181 ttacatgcct gtccttgcgt ttcattttga acttggctcc attagcagct gcctatctgc
      241 atctcgaggt ttgccccttt ccatgccttc atttaccaaa tgacccaaca tcccccgttt
      301 gtgtcaggct tagacagctg agctgattaa gaagttgcct tttgagcgct aatttattcc
      361 actccaccat tttgttatat ggccattgtt tcatttaaag tttagcctgc ttctcctcag
      421 atggaagtca cgctaattct gatttccttt tgggaaagct atttggtttt acaatgtatg
      481 atggatctga ttagtcattc aggccggcct ctgcttttat gcttacatta agtaacacaa
      541 agagcccccc tacaggttaa agtttaaatg tggattagca gataaagcaa ctcctgatta
      601 ggttgctgca gggcttagag ggaaaaaaaa tggattctcc ctgcacatta tttcacaaac
      661 atcttcactc tgccttcttt ctctccctct cttttcttct ccggcttcat cctctcacac
      721 tcagtcccag catggatatc tattttggcg caaaatcttt gaacaattat gcaaatgcat
      781 gtaaggcaat gcacaataca gtatggaaat ggcttggaga gaaaatggac tcattaagga
      841 cagatggcat gagcgctgaa agccatttgt tctggatgca ggaagacatt tcagacaatg
      901 tcagaacctg agagacatca gatatgtttt ttaacaccga gcactatttg ttgtggtgct
      961 gtagctgttg attatttcag catgttgatg attataaatg agcacagact tttctctcag
     1021 tgtggtgtga ctgcggcgta tagatgtgca tttgttgaaa tatacatttt tgtttaagtg
     1081 atgacagact tgtaacattt cgtcatcccg ccacatttcc cttttactgt tgtgttaaat
     1141 gatgtcattg atcaccacaa gaccttttaa caaaatcacc catgtttgat caaagcataa
     1201 tcttgcttcc atatgttaaa tatggaagca tgcattgtga tacgatcgct gagacagaaa
     1261 tgaacagaga cgtggaagca gttgtgatgt gcgcttgtga aattacatgc accaaatgca
     1321 tgacttttgc actgatatta catgcatttc attttcactt catacgacat gaattgaatg
     1381 catataaggg gggaggaccg aaatgcttgt gagagattac agtttccata tatagaaaag
     1441 tcctttg
//
LOCUS       KCVB01000543            1403 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25672pri ultra-conserved element locus
            uce-380, sequence.
ACCESSION   KCVB01000543
VERSION     KCVB01000543.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25672pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1403
                     /note="ultra-conserved element locus uce-380"
BASE COUNT          441 a          310 c          327 g          325 t
ORIGIN      
        1 gaaaattgaa tcccccccac caccacctcc taaactgcac tgtggaagag tgtatgacag
       61 gccaggacat gtttcctctc atagaaagtg aggcggaagc ctgaatgttg agagatggaa
      121 aaaatgtcgg aggacatggg cgtcactgtg agttgactga cagccatgac agcgttgaga
      181 tgtcagaagt gggctttaca ggagtcaagc agttcaagga cgcccaaaag attggctact
      241 ttcagacaca tcatgactga tcctgcggac cgtcatcttt atagtttgag gatacgtgga
      301 caaaataaaa aaaaacataa ttgctacaag aaacaagcaa gaaatatggt atctaaagat
      361 aaacagcagc tagagtgata acctagtgca cgaccgagga aggatcttaa atctctactc
      421 agctgaaata aagagaggat caatgagagg aaatctgagg ggagcaatgg agaaggtgga
      481 aggagatgag agactgagag ggaaaaaagg ggaggaaaaa aagagtgcgg gaggaagcaa
      541 gagaaacaga gcaggcagag agcaggagac cgaaaggagc gagcgcacac aaaatgagaa
      601 aatgatcagg ttcacaagaa gcgaggccac caaatttgtc gttgtgtaga agaacccctc
      661 attaccgcac ctcatgggca ctcggcgtgg cctttgaagt gctttgaggt acatcatccc
      721 atgaaacgag catagttaat ccacttgtta atcaagtgtg ctcatgagct tatgtctgaa
      781 gtggctcttt ctcctcagaa gactcaaagc gttcaaagga aattgtggcg gggtgaaaaa
      841 agacgaggca tcgaaaattt cagacaaaat ccgagtgcag ctgtgtcaaa acatgcacaa
      901 tatcccaaac aattctgccc tgcaacaaac cccgatgtct ggaggggtgt tttggttcca
      961 tttctttaaa aacaatcata atcttaaatt tgcctcaact atgctagata tccccctttt
     1021 taatattttc tatgtactgt ctatttaaga taagcatctc tcatttatta caacaaacaa
     1081 gtgttttcac tctgtaagtt cttgtataaa aaactgaaat gttaaagagc catttgggaa
     1141 gggtggcttc tcatgcttgt cttcctcttt cactccccat ccattcctga tgaatgggtt
     1201 gtctcccttt ctctccacag caaacaggaa aacagacagg agagagcgag ggatggctgc
     1261 ctcttcctca ctccttctct ctctctctcc cccaaagcct gtgggccgct ctgaggcaca
     1321 gccacggagg cccggcgctg cctccccctg ctgcgccaac aagtctcctt ttcacttcaa
     1381 ttaatgagcc ataaataacc caa
//
LOCUS       KCVB01000544            1409 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25713pri ultra-conserved element locus
            uce-751, sequence.
ACCESSION   KCVB01000544
VERSION     KCVB01000544.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25713pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1409
                     /note="ultra-conserved element locus uce-751"
BASE COUNT          412 a          316 c          292 g          389 t
ORIGIN      
        1 ctaatgcaaa tgcttgccgt ttaaaaaatg ggagcctctc tcctactctg tctctcctct
       61 ttggaaaggt ctattttggc cttctggtgc tggcagttat ttttttcctc tccagaagga
      121 cagagagcca gggagatgca cctgtcatcc attttgaaat tcaacattat tgatctggtc
      181 atggggaaag gaatttataa agttatgcat ttttcttcgt taatacttat tgatctgtgc
      241 cttaaaacat gcattttttc ttatttgcaa agtgtttcac taccttattt atgagcatgc
      301 taatttggtg ttcgcagcac agcaatacac agagagaaca cacatacact ctcacacata
      361 cacacaccag tcaccgagca acatcccagc atgccctctg tctctgcacc cctgtaattc
      421 tacatattgc ttaaagtatg tgctaattaa attttctgtc ggtttgcaag agaaataaat
      481 aatctgcagc taagaggcct aattgcgttt gtcacgttgt gtctcggtgg ctgggacatc
      541 gggagcacag ccgctgccct tgctcattgc cattctgtcg tcctggcaag cccccgcgct
      601 gccccaggta tcaccacgcg gccatctgga gccgacatct gtctctctat tgatcacaag
      661 ggtttagcac ttgatcaaac agactgcgac aggaaattgc tcttttcccc acagaaaagg
      721 ccaatcgcct gcgtgagggt aatagagtgg aactgagatg cagcaccagc gcggtgggca
      781 agcgggcacg ggcacagcca ggcaggcagc ggcacggcgg tgtcagtcgc tgtggaggtg
      841 gcagggatgc taaagtccac tgccatacca agaccatgtt tctaacctct ctgctattac
      901 taaaacagca gcactcataa tatcacagta atcaaacctt tctaaattat aatttacagt
      961 caggcaaatt ttgaggttcc tactcagagc acatcactga caattctgtg tacgtctgca
     1021 gaagatgaga ccaacacatc tgattaaaaa aactgtcatc tgaagaacag aattcatctt
     1081 tcaagccatt caggtaaacg tctatgccaa cagctgtcta aaactgttag aaaagctgga
     1141 tgcaagtgaa atatgctaaa tgataagcac accagatggt ggaaaattaa tgtattactt
     1201 ctggaaaaaa aaaaaaagtc taaatacttg agcattaaca ccaggactaa attttaatat
     1261 ttgtagaggt cttattgtca gttttcataa caattaaaat acatgacagg ggtaaaacct
     1321 ggcagtgtgc aaatctgttc taaaaaatat gtgtatgaaa tgtgattgag cacctgggtc
     1381 tcatgcttca atagtgggaa atccctgac
//
LOCUS       KCVB01000545            1502 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25759pri ultra-conserved element locus
            uce-847, sequence.
ACCESSION   KCVB01000545
VERSION     KCVB01000545.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25759pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1502
                     /note="ultra-conserved element locus uce-847"
BASE COUNT          452 a          311 c          325 g          414 t
ORIGIN      
        1 aagctctaca gcaacaacct taaaaagggt tgtaaggttt aggttgttag cacaacagaa
       61 atcaatacat caatactgtg tcgatagaat ccatctataa acgggcaatt gtttggggga
      121 tggttttcaa gtgtagatga ttctcatttt aggaacgatt aaacaaacaa gcatatcaag
      181 tgaaaggaaa atgtctgttt tgggagcgag tccaagcatt tgcccttgta aacacaaagc
      241 ccacatatga ctgggcctca gttgggcaga cagcagaggg agttaacaaa gctaattagg
      301 ggaggctgac atgttaaagt gagctgcggc tcgggagtca gctctatcac atatgtttcc
      361 tttgtggcgg gtgaagtggg tcctttgttt gtgaattgat ggcgtttcgc tccgccgtcc
      421 ctttgggtag aggcactgac ctgcggaggc cccgacagtg agaggaggct ctttctctgt
      481 ctccccaatg cctgctgctc cagctgtccc actccctccc aaacctaaac cttcccagag
      541 ccgtggatgc acagtgagat gggcaacacc tgtaaaaaag tgagtacccc ctcagcccag
      601 gcacctcaaa gcagcctgcc cacctgctac tgagctctct gggccttgca gcttaaacgc
      661 tcattttgtg cctctcagca cgggacttat tgatataaat agctgtgcac aaagtgcaca
      721 ctctgttttc tcttggtctg atgcagtagg tgtgtatgtg attatattca atggcagcct
      781 ctttcaggtg gggatgaaat atgagcagtt tggacactgg gtaaatgaca atctctgcta
      841 gattcactga aaatgattat agcacaattg acatatatcg aaatggacaa catgattctg
      901 gcacccgtct taaacaagga ttcaatatct aatatgtaga cactatatac tctgtattaa
      961 acattacaga tactgcgata gatttaatga tgaccttacc atttgtcttg atatagcttg
     1021 tccttcctcg gtggtgaaac gtaaggtatg tccttgaaca agacactcta tgggcataat
     1081 gaaaacagca aagtataaag tccatatagt gcaaacctgc aacttattat tatgttaatt
     1141 ataattcaat atataagtag ccaatttatg attaactcaa tgaggttaaa tttatatttg
     1201 aatcagatag aaacaaaaca gtagaacaaa tcttactgta cagcatctca aagcatgaac
     1261 tttaaagtga taatttgccc tctggtgcca caaacaaagt ttagaaaaag ccaatctctt
     1321 tcgttcacct tagcctgcat ggcaacagat taatcctcac ccattaagat gacaagacaa
     1381 tttgtggagt agttgagtgt aaataaatac attgaggagg agataataca ttaggctgct
     1441 gaccgggcta ttccattaca gcagcacatt tgtttccata cacggaggaa gccattcagg
     1501 gg
//
LOCUS       KCVB01000546            1633 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25808pri ultra-conserved element locus
            uce-490, sequence.
ACCESSION   KCVB01000546
VERSION     KCVB01000546.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25808pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1633
                     /note="ultra-conserved element locus uce-490"
BASE COUNT          491 a          385 c          324 g          433 t
ORIGIN      
        1 ggcacattaa atccctgctc tgcatgatgc ccttttccac attttaattt agccttgtct
       61 atctgttgta tccagtaatt ttcctgacgc ctcatgatga cacatcagca ggagtggtgc
      121 cttagcctat aaagcaacat gttcccttca acacaaatca aaagcatata tcaaaagcat
      181 atatcaaaat gcccaattag gttggatgta tctacactct ttaatgtttc cagctcacgc
      241 tgtcactatt ttctagcacg gcctagcctt ctgactgagt ttgacaccca tggtctatac
      301 gatcaacgtc aacagagcat gtcctggggc taagtcttac tcagccttta tgagtaagag
      361 ttttctgctc tgcttgcatg acttttgaga gaaaaaggca gagaaacata gtgagaaaga
      421 gagaatgagc atagctaatc cttacagtaa tgacagaata attagcttgc attaggctac
      481 gtaatgggtt gtaattatat catttattct gcgttgagag aatatgataa aaagcctgca
      541 caatgaagga agaagaattg agctcagtgg gaagtaggat ggagcatgtg taagagctca
      601 ggctctttct attgattgat ccaaacatca aacgaaccac tggagtaatg gggggagtga
      661 aggacagcta aaagctattt ggagccttca ctgcataaaa gacttgacct gttccacaca
      721 gcctacatat caaaaccatc aaacagcaat caatagacca aagcggtcaa ctcaacaaac
      781 cgtcacccta ctaaaacatc aaacccccgg cccaagcgtc agatgatagg ctacactcag
      841 cttatccatt accatcagat aaagaggaga taaaacacag agcctaatcc tttcatgtgt
      901 cgtgacagcc acagcagcct ccaaggcatt ccttcagtct ggctctcaaa tagttttatg
      961 gcggaaggca gccattcagt cagccagcca gccagtcagg gctgttctgg tctgtttgtc
     1021 ttatttcaca gtttacacgc tgttttatag gtagttatta tggtcaccat ggtgatgcca
     1081 tacgttttta tggtcttttg gtctgagggc agggagtgta ataaacgaaa aaacacacac
     1141 acgtgaaaac aaagactcac aaaacacact cctgctgtgg aaaggtctcc tgtccctccc
     1201 ttactccctc ccccactctt tatcagtctc accaaatgcc tatctcccta ctttctgctt
     1261 catgtgaaac acggataggc tagcattgaa ttgtcctctc tcataccaca tctggagctg
     1321 atcagagggc ctcctggaag ttgaggcctc tgggcctatt aataaccata ccgctgaact
     1381 cagggtcaga cagcagtcag cggatgagcc tgtgtctact aaacacatag tcccttcccc
     1441 cttctcaaga ggacagctct cacttttaga aatgagaagg taggctatag agcttagcag
     1501 tgtacatcga gttttacgct tttaaaattg aagcatacag agattcctcc tactcaagca
     1561 ggagctaaca aggtattcac aaataagtaa acaaagacta aagtttgaaa cagactatac
     1621 attcggtcat cct
//
LOCUS       KCVB01000547            1207 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25887pri ultra-conserved element locus
            uce-45, sequence.
ACCESSION   KCVB01000547
VERSION     KCVB01000547.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25887pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1207
                     /note="ultra-conserved element locus uce-45"
BASE COUNT          345 a          302 c          228 g          332 t
ORIGIN      
        1 tatttaagac ctgacttgct gataaaaata aatgggtggc actgaacgca aagatgtgag
       61 cgcttatgtg cactgctgca tccattaaga ccttttgatt tcactgcaat cttttgatag
      121 tgaacccaat aacccacgca gcttttgtat ttgtgtgtca cagtatgaga aacagtgtaa
      181 tgtcccatag gcttgagtac tactcatttg aatgtgttta acaaaacaga cacagttgat
      241 attaggtcaa cattgtgcca caggcaatgc agcaaaaaga atcagatgtg ggtgggagag
      301 cttttcactc tctgctttta ttctttccct aacagttaaa aggatctagt atgcatttaa
      361 gcaacataat agtacgctac ctcaccagct tttatccagt cttaagctat agtgtgcggg
      421 acccacgtgc gcactcaatc gcgctcccgt acacacaaat gtaaggtcag agagccaaca
      481 agccagaaaa gcttgtaatg gagagcgttt atgggtagta atgcaaccca aatacaccac
      541 cactcctgct ccaaatgtga tcatttaatc aaagtacaga tgcacaaagt cttcttaaat
      601 aacctttgga tttggctgta tttgggaatt ataaaggcaa agctagaggc tgagggccaa
      661 agcagatcac agggaaggga ttttctgcat atcctgatac agtcagtcag tcgctgggca
      721 gggcaggaac tgtccatggc cagccaatgc acccagtgga attaattcat caaacactga
      781 acagtaaagg gagggttctt cgtaatgaca aatcctggtt ttaggccacc aaaacgtatc
      841 tacaaagctc cataaagcag ccacagggag gagctaaaga gtagatccac ggttgcactt
      901 aaaatcatat acaactcaca tacacgcaca aacacacact cttttgtacc atgaaagcca
      961 atgggtgcaa ccagtgacat cagtgagtac tgtaggactt ctgaaacaag cccagagcct
     1021 ctaagcccct actgtacttc ctccatctca gacaggcctt ttcatctctt atcatcttct
     1081 ttcttttgct tctcactcaa ccggctcctt ttctttttcc accctctgcc tgcctctatc
     1141 ctttcccctc aacccgtttc ccttcccatt gccctccact ttttcttctt tcatcttctt
     1201 cccccct
//
LOCUS       KCVB01000548            1909 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25929pri ultra-conserved element locus
            uce-729, sequence.
ACCESSION   KCVB01000548
VERSION     KCVB01000548.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25929pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1909
                     /note="ultra-conserved element locus uce-729"
BASE COUNT          564 a          343 c          355 g          647 t
ORIGIN      
        1 atacattttg ataaggggga tttcctgcgt agacctgtga ggggaaagaa gaagttaatc
       61 catatgaagt gtgttcattg gttcatcata atcatccctc aatgcatagc agcatagcca
      121 agaagtcatc tgcatacacg ctcacatttc agcttttatg caaatggctg tgtatttgac
      181 caaagagttt ctgcagtttc tcacatgctt acagcttaat atgtgtatat acccttgtat
      241 acttcccttt tgtctgtgtg ttttgagaga ttttgccagg acgaggcaca tgatggtctg
      301 gatctggaaa atacaggcag tctagttagt ctagacagat tgctcatttc aaatagaatc
      361 ctacattaac aagtgttaaa aaataccctt gaactttcat taaatgactc ctaggtaaaa
      421 ttgagaacac acaagtctct tctaggtgag ctaatgtata acttttgaag ttttttaaat
      481 gtgtaaataa attctgatac gaaccatttt ctaaaaactt agattgtaag atggcaatgc
      541 aattagtatc cagcgatagg gaagaaattc aaacaaacaa acctaatttt tatggtttag
      601 gactgagtgt ataacagcgg aagtgcagta acaatgtaag gcttttacaa tttgtttaaa
      661 agaaaaaaaa aaagaaaatc tggtcatgta acaatagaag tgccttgatc cagcaaacct
      721 ctcaaagaca tcattagtgt tacactgtaa actggtcatc cacttcgact gccgtgcaca
      781 ggcttttaat tagatgcagg ggaccggcat atgaaaagaa gcggctagag gctaacattt
      841 aatgagctat ttgttgcttg atttcttttg ctaccgtatg tgaactttcc caatgcaaat
      901 ctccttgttt tcctttttcc agggtttatt gtcatcccaa aaccaagcca gctctcccag
      961 tggaacagta gtatagccac aatatttctg ctgctaaaga accctgcaac ccagagtctg
     1021 atgctgctct tcaccctgca agcattgcaa gttttccttt tctttgatta cccaccccct
     1081 tccactaccc cgattttgtt aatggctttt ctgttctttt tgtttatcac tcacttgacc
     1141 tagtggagga atgaagcaag aaaaggtgtg gtgcaattgt gtactattgc gctaacgtac
     1201 tgttgttctc tggagtttta acacatttta gagttttaaa aatctttctt taatgtcgaa
     1261 tctcatcaga ctgttgtccc ctctggaaat aaagcttggc tttgttagat gcaagctaga
     1321 caattgtggc ttgagattaa agagatagtt catttaattg tcatagtcac tgtgtaccct
     1381 tgtggtgtag cccagtcttg gctttgggtt actgacataa atatgacttt taggtgaact
     1441 gtcctcttat gaccaagttc gtgagcatca aaattttgca tctatccaca ttcacatgtc
     1501 ttatcttaag tagaatctcc cctttatgac tttgcactga ttttgttagt tattttgttg
     1561 tttgtttttt taactactct aatggaacaa aaaagagggt tgaggactgc cttgttgcac
     1621 tcggctttaa catcctcagt cttgaaatct gaatctataa agctggatgc tgcaatcata
     1681 gtctttttaa agaaaaaaaa ttgtttgcac ttaaattagt ttattagaag aaaatggctt
     1741 tacatttttt gggtgtgctc agaactcaca gattgctaat acagtaggtg aagaaaatga
     1801 taaaaactat taaaaccttt atctattgag catttatttt aatattattt cagattcaaa
     1861 tctgctctgt atcataatgt gtatattgta attaattgat ttgatgggg
//
LOCUS       KCVB01000549            1391 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 25964pri ultra-conserved element locus
            uce-1073, sequence.
ACCESSION   KCVB01000549
VERSION     KCVB01000549.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="25964pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1391
                     /note="ultra-conserved element locus uce-1073"
BASE COUNT          505 a          215 c          266 g          405 t
ORIGIN      
        1 tgacataaat catgcagaac tacagagaaa ttctgtatgt gatcagtaaa gatttgtaaa
       61 ttacaaagca atgcatagga tattgaaatg atctcagtgt gcttcattta gtgtgtgcaa
      121 aaaacccccc tgatataatt taatgaaaat gtggggttgt atttgttttc aaatatgtgc
      181 ttttatgtgg acaaaacaag tgtctttaca tgatggatac aaattattta ctgtagatct
      241 actgggagaa aagcgcagag gttagacata ttatttgata acagcttcaa ctaaagatga
      301 ttgaaaaagt ccatgctaat gataatccac tgagtgacac aatgtatgac cttgggagga
      361 cacacagtaa acttgcgcat cacaaagact taaacaaact cactaggagg gcaagatggc
      421 tgcaagcaag aaaaccatct tggtgaaaat gaacaattaa aaacaaataa ataaataaat
      481 aaaaagtaaa tttgcccaaa aatcacagaa aaggaaaagg aacttacata aaagtatata
      541 gtgactgggc aaatgatgac tgcacaacat ataaagatgt tgattgttgg ataagggtgc
      601 ttaccttgag attgtctctt agaccctagt tgtgttgtac tctgattcat gtttgctcaa
      661 agagccaatg aggcagaacc tgcaatgtca tggctctttc atttaatatc aaactccttt
      721 tgcatagaga ggtcaaaatg caaaatgtgc tacataacag caataaacgt cactggtgtt
      781 tttgctaatc tctaggagcc ttgcatgcag acttgtagaa aattaataaa caatcatgaa
      841 cactctggaa agagcagata gaaagtgcat gcgggagaag gtcacatgtt caaagtattt
      901 tctccaaaag tgtactctga taccttcaca tagtgtgttt gtaaacatat acagttactt
      961 ttcaagcagt caaggtgcaa gtacacagta aaatacaatt ttttttaata tagaaaaatc
     1021 tttttcatgc cgaaatatta agctataggg aatatagatc atttactttc acagctagaa
     1081 atagaaatgc atactttatg ataatttgca ttcatgtcaa taaagttagt tatctctttg
     1141 aggcacagat tgaagccctt gtgttaacag tccaaactag actgatgaca gaaatagtat
     1201 cttctgaaat gaggtctgta tttgctgaaa gagctctgtc aaacctcatc atacagtaga
     1261 gtttatagat gtaagctacg aatcaccaat tgaggaaaac tagcttgatg tgttcaagga
     1321 taagcctgca agagggggga aaatttttct tatcataatt tagccctttg tgaaaaattg
     1381 agaaaaacgt t
//
LOCUS       KCVB01000550            1637 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26008pri ultra-conserved element locus
            uce-1036, sequence.
ACCESSION   KCVB01000550
VERSION     KCVB01000550.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26008pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1637
                     /note="ultra-conserved element locus uce-1036"
BASE COUNT          423 a          327 c          364 g          523 t
ORIGIN      
        1 tcacacccct cggcgatttc tccggaagaa aacctccgct tttcccttgc acttcctctt
       61 tccgtcaacg gagtttaggt aggacagcta ctttctgatt cccttcctta aatctgctct
      121 aaatcatgaa tttgttagtt catccacctt aaactaacgt ttctaaatgc gaagaaaatg
      181 tctaagaata tgtcttcctg agtattttaa cgacagacct ctgttttaaa gcattttatt
      241 ggttctgtgt agctcattga tgacatggcg ccgtaacagc ggtgtgcact agctgaaagg
      301 ccggcagccg ctacagacag gacacattag tgtttgtcgt aaaccgttta gaaaagccag
      361 agataattta gtgtgtacac acctgtcaga gctgacaata aagtgctcta tgtctgttta
      421 actacagacc gctttgtgcc ggtactttaa catacagtga gctatagtaa gttacggtgg
      481 ctatgttgct aagctaataa cagctaacaa cttaaagtct taaaaacgtg aggtgttttt
      541 cgtttgcgac tgccttttgg cgtatttctg aattggtggt ttaagtttac gaaacgtaaa
      601 gtgtaattgt gatcattaag gctaactttc tgacttacaa gtagccagag gcctcaaagt
      661 tagcattcac atgtcaagct ggtaaataac gcgaactgac gtctgttcca ggacacgatg
      721 ggacgtgtga tcaggggaca gagaaaaggt gcgggctccg tgttcaaagc ccacgtcaag
      781 cacaggaaag gtgctgctag actccgtcac gttgacttcg ctgaacgcca tggttacatc
      841 aaggggattg tgaaggtact tgagcactcc agcattgttt ttgacagcat gggaaactca
      901 tactggttgt tagtgagtga acatttgtga cattgctttt ctttggtggt cttccctctc
      961 aggatattat ccatgacccc ggccgtggtg ctcccctggc caaagtagcc ttccgtgacc
     1021 cataccgctt caagaagagg acagagctct tcatcgctgc agagggcatc cacaccggac
     1081 agttcatcta ctgcggcaag aagggtaaga ggctaagacg actttttagg gttcttccag
     1141 cataaagcag atttagaagc tacacagacc tgttttacgt atagtctgtt gacccaccca
     1201 aaatctcgca ctgacataaa gccagcttta gacatggatc tgtaacattt ctagcttcat
     1261 ccgacgttga aagaaagctg tttggtttta gacatttgtt ctgttacgta agtagtctga
     1321 gatggtttaa atgtgacagg agcattgcag atgcagatgt gttttagcat atcaagtgat
     1381 tgcttttgaa tctgatttgt ttgacatttt tgacatcttt ccggtgactt tgtattttca
     1441 aagtggtgac tggtgacttt gcattcatgt tgcttcatta ttttttttat tgaactcaat
     1501 tttatgtaat aggtttttat gttgttctgt agcttcccag taatgggttt tttgggtatt
     1561 taatttgaac aatcaatttt tgttcaaagt agttatgttt tagcgttaaa atgtttaccc
     1621 ttgtgacatg atgtagg
//
LOCUS       KCVB01000551            1321 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26082pri ultra-conserved element locus
            uce-340, sequence.
ACCESSION   KCVB01000551
VERSION     KCVB01000551.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26082pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1321
                     /note="ultra-conserved element locus uce-340"
BASE COUNT          379 a          298 c          297 g          347 t
ORIGIN      
        1 acacagacaa gtcatttcca tttgcttttc cttcaatatt tatttgggtt tcctctatca
       61 ttttgcatga accacactag aactgacagc cgtgaattgc agaagcccaa aggctgtcta
      121 agaacccaaa atataaacag gatgaaattg cccaaaatat gcaccggcca taacggtaca
      181 gtgctgtcat cagagaggtg agtcatctta tagagaagtg aaaaggaggg tgggagagga
      241 tgcatgcaaa ttgactaaaa ccttaaagcc agtaacctaa ggaaattaac ccatatacaa
      301 aaataaataa caccgtcttt gtctcttttc caacatatgc atgtgtttga catcacatgt
      361 ttatgtattt tgacatgaga caacactaca gataacgatg atgatgcaaa cgatcttttc
      421 ttacccgtgt gcgagcgcaa gtgtcttttg agggtagtgt ggctggggaa ggggcgctca
      481 cagtggctac agatgtagct gtgcatgccc gcatggacct ccatgtgctg ctgcagggcc
      541 ttctgggtct ggaagcgctt tgcacacagc agacagaaca ccgccatgtc agtccctaga
      601 gaacgcacaa gacacagaca cagttatttc agtaccatac acagacaggg ccttaagtgg
      661 ggaaactggg tcacttcaac tttgtgtgag tcctctgagg gaaaagcttc tttcaaagag
      721 caagtgcctc tacctaaact aactccccta aaagcccaga ggcactttca actgtgagtc
      781 tttatacgtt ctattttaaa ccatgaaagt cattgttttc tttaagaatt tcccacacaa
      841 tgcacagcat gccaatcaca caatcaatgg cttcttcctg gtgtgttaga tgtgtaatta
      901 ttctcttcat tccccatcag gagtcagctt cagcctgtga agtcctctgt ctctgtgctc
      961 aaagagcggc tgatagtagc tccaccctaa atgatgccta cactatgcgc tctgtgcaga
     1021 atgttaagta gtgatatcaa gccattaaag gcaggactgc aggaaaaaca caatccagtt
     1081 taatcctgtg tgtgacctgg catctctttg gggctgcttt cctgttgcac agagtgtgga
     1141 gaggaataca gatggggaga cgctggcagg gtagctcaag tcaaaatggc tatgtgaaga
     1201 agatccattg gctgtcatac agtcagcact tgatctacag gctgtgtacg ccagcactca
     1261 aatccattct ggcattcaca ccgatgcaaa ttcatacgtg tggtaagact gagggactgg
     1321 t
//
LOCUS       KCVB01000552            1483 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26123pri ultra-conserved element locus
            uce-791, sequence.
ACCESSION   KCVB01000552
VERSION     KCVB01000552.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26123pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1483
                     /note="ultra-conserved element locus uce-791"
BASE COUNT          464 a          315 c          309 g          395 t
ORIGIN      
        1 gaacccaatt tacacgctgc tccattggag atgagcactc taccgtaccc gagctggtaa
       61 tcttgcccag attccccctt tgtgtcctgc attattgttg aaataaataa cctttcactg
      121 caatcagtgt ggggatcttc acttaaatta tcgccccaca gatagctcac tcatgatctg
      181 cttctatatc cattaactat ccgggaaagt gatttattgg tgaaggataa gaataaagga
      241 atgagcagga agggaaggga gtcattttgg ttagacaata gtgccctcta ctgtttgcaa
      301 agtggatcga tttgacagag gaggcaatgt ggaaactggc actgataaca tcttcaacac
      361 tcatgtgctg gtgaagtttt gggaaagggc tctgtttgtc ttcttaccta aaacgagctt
      421 ctgtgagccc tacaatatgt tcattgttaa aacgatgaac taaaggagga gcaatcaaaa
      481 gcaaccaaaa agaaatccag atccagagta aagcatcaga aaagccacta ttaacaattc
      541 tcttctgcaa acagcgcaga tcttaggtac agctcagatg gaagtatggg catggacatt
      601 atgccctctc tcagtagcaa ttcaagccag ggctgcagca cctaaccccc cttcatccct
      661 ccatcccctc ctccgcagtc cctccttccc ctcactcctg tgatgtgtcg ttacacgctc
      721 ggctggctcc tcttccatga cagactgtta cttcctggag cggaaacgca tttgattgtt
      781 aacatctggg ccattacaat tgcaaactag ggctctgtac atcaatcagg aagtgatttc
      841 aaaacaaaga gaccgtcctc caatcaatct attgggaagc gggggcatta catgctcctg
      901 taatcccggc taattagcgg gataatggga atgagattta tgaatctgct gaggagagtg
      961 gaggtgcgag ggcacactca aattagcagg gatgcacgcc agccagggct gcaataagga
     1021 ggatgtgctg ttggttttta tcaacttctc gctgcctggc cacctttggc tcgatgtcac
     1081 tgtgaaagaa gcatcgcaaa ctaatgttca ggaactatgg agctaaggca ctcttacagg
     1141 aggtgaactt ttatgttaaa agccacctgt tgtatctgtt ataaggattt atttaagcaa
     1201 aaactgattc acaacgcaac atatacaaac taacatcaat attaaaagta aataatatag
     1261 gcataaaaaa acttatctat atgtatctaa gagagaacaa cacataggtt ttacactaac
     1321 aggtgattcc ttgtcttgtt tttcaagtaa gccaaggata tgaacaccag ctcaaataga
     1381 tatgatgttt agaatagcag tagccacaga ttactgagtt gaatatggat atgatataaa
     1441 ataatgtgaa ataaaaaaat caaaacaaaa aaatattctg act
//
LOCUS       KCVB01000553            1293 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26161pri ultra-conserved element locus
            uce-428, sequence.
ACCESSION   KCVB01000553
VERSION     KCVB01000553.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26161pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1293
                     /note="ultra-conserved element locus uce-428"
BASE COUNT          447 a          244 c          280 g          322 t
ORIGIN      
        1 ttattgttgt caaaggggta acaaagtata cagttaagga gcaataatat tatagtttaa
       61 aagatgtaaa ttgtgggtga ttgaccattt ttttgagcag aacactattg atgcatgaat
      121 agaaacttgc tgattgtggc tttaaaacaa aacccaatgg tccctgtcca ggcctggtgt
      181 ctgtatatga gattataaag ctgtgttgat tctatcttcc taatcccccg tagctttcca
      241 aagagacaag actgcagaaa ctccacaggg attaatttac cattcacaga ttagagatcc
      301 ggtctccaat atcatcatcc agaacaagca ttgagaggaa ggtcttaaca atgcagcaat
      361 gaggagacag agaacagaga gaatgagaga gagacacata cacagactgg aagaaagaca
      421 gagatgaaaa cgaatgcaca catggagaca gccttctctc ctacagcatc atgccttaat
      481 gtcctagctc gaagacaaga acattagacc tcattccacc tcctcatctg ggcctgaaaa
      541 agcctgggct gaaaatctta attaattgtc caatagtgct gcggggtcat tatgcattgc
      601 aataaagaag cgtgtaatta tgagatttaa cagccattac caaggccagg aactaatatg
      661 tcatcatcag caaccaagcc aatggtttgg tacggctgtg tgtgtggctt gatggacagc
      721 acacacacat atatgataca tatacttgca tatgtacatg ttgttcatgt aggtatatag
      781 tatttagata tattagaaat gtgcctgctc ttgtacaggc aaacaaacag ttgtaggtat
      841 ataaataggt gtgcagacta ttaagggaac aaactaaaaa tgcatacaga cagaagcaca
      901 cacaaccgca cgcacacata accatcagtc tgacccagac aataaggcag ccagctgtga
      961 tagcagcctg cagatgaaac aacccatcaa gattaaatag acacaaaaat acacacatta
     1021 ttacagctgt gtgcttgtgt gttttgcgtg tgcatgtgaa tgcaaagtga agcgctgaga
     1081 ccacggaaac agtccgtcgc catatagcag gtcggaagtg acacacttgt gtaaggatgg
     1141 gatgccgtgt accaacgcgt gcatatgatg tgcattatta aaaaagtgct aaaaaagtcc
     1201 aacaacaacc agaagtaaaa agtacaacag gaaacatatc tgagtggtta taaagagagt
     1261 gctttgaaga gtggtggtta ttattttgat cat
//
LOCUS       KCVB01000554            1326 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26199pri ultra-conserved element locus
            uce-1337, sequence.
ACCESSION   KCVB01000554
VERSION     KCVB01000554.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26199pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1326
                     /note="ultra-conserved element locus uce-1337"
BASE COUNT          373 a          243 c          345 g          365 t
ORIGIN      
        1 ttttgctaca ccaaaatgta aagaagaaaa agaagaaaag tgattaacgc atctgagctt
       61 aaagccggac aactgaagac acagcagcgc atcagtacat ggtcattaaa aattaagaaa
      121 gtgtacccca atatttatag aaaaagattt tggatcaagc cttgttttac tggatctccc
      181 taatgagcac ttagactgat aaacattgac gttggtttgt caataaaaaa gccattatga
      241 gcttgcaaaa gatgctgcta ttgaactata accatactat gttggtacat gttgtatggc
      301 tctctatggt gcagtaaagc tggactgtag attcaaattt atagtgataa tttcattatt
      361 ttattccact gcattagcca tcatatccat tttgcatgaa tttcttaatg ttatgctaaa
      421 ccacttattt tatcggtatg tggacatttt ttttcacatt cagaagtgtg tgtttgtgtg
      481 agttcatgaa ttctcctgag agtagatgac tagccagaag tatggaggga ggaggtggga
      541 gggtgtccag agggagctgt ttgattgatg tgttttgggg gtagaggaag gtgggggggc
      601 tggggtagtt ttgaggatga ggaggggata ggagcttgat gggactccgc ggcaggaggg
      661 aggcagggct cttaagtggg ggaggagggg atgggagctt taacaagccc tagattgtta
      721 gtgacaggca gatacttcaa cacttaaagt agaaggtttg atttaatcac agagctgtca
      781 tttgttattt gtctggcttg ctccaccgag gccacggctg atcaactgcc tggtcccaag
      841 agagcccttt ttccccctgt aatgtctaaa tccccagcac aaattacagg ccagcaccac
      901 ctccaatccc caaccctcca cttccctccc tgcttcaatc ctgccatgtt gcgagacgaa
      961 gtcctgaatg gggggtttgc aaaaagagga aggcgggttg gcaggacgtg agcagaggat
     1021 ggagacaggt actgggaaag agagaggaag atggaatggc ctgagggcca ctaaagatca
     1081 ttaataggac tgtcagggag gttgccagtt ggttagtgtc tcataaataa gaaatgagcc
     1141 cctccctcct cgcccattct agttcattaa taatgcctgc tgtggctatt ttgttgcatc
     1201 gcactttgag gggagcagag tcagagagat ggagcatgat gagggggatg caggaataca
     1261 ggcctcactt ctctgctctt tttggtaatc caataagata atccaattat acattcacaa
     1321 tatgtt
//
LOCUS       KCVB01000555            1635 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26237pri ultra-conserved element locus
            uce-218, sequence.
ACCESSION   KCVB01000555
VERSION     KCVB01000555.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26237pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1635
                     /note="ultra-conserved element locus uce-218"
BASE COUNT          423 a          414 c          331 g          467 t
ORIGIN      
        1 aagagaggaa acagtttctt gctcacagat taaaccttgt actctccgat tattgaacaa
       61 tgagaaccct ccacccgcac tccatccctg ccctcctcct cctcctcctc tctccactac
      121 actgtaatat cctcctcatc cacacacaca cactcacagg acttattacc agctcattag
      181 cctgaatcat acaagaatta gactccagcc cgctgacaat agctcagaga acgggccttt
      241 ttcggctcag gagtcagggg ttaagttacc aatcaaaata gccaggcaga tgtattgaga
      301 cattttaata ttggccattg ttggagctct aaaaaatttt aatctctcac tggcacggct
      361 gccccccaag gcaactcaaa taacagtgtt caggaggggg gttggtgttc aaagaattga
      421 tgtaccctgt ggcatttgtg taattgtgtt ggaattgctg ccaccttgaa cagttttcag
      481 gctgagcgcc ctttcttcat tttacttctt tgcttacctg gaccatagat ttttctgctc
      541 tctcaccccc caatgctgaa tttctcttca tgttccactc cagtgtccct tcccctttaa
      601 gtccctcaaa cccaagaagg gaaattacac taggaaccag tacactgact gtgttagcgc
      661 cgtcctgaac aaagtgggcc aggtgatgct gccgtaccac agttaatata atgtaaacca
      721 ccagaggggg ccaatgttaa ggagtgggcc agaaggattg actgcaattc acccagacat
      781 gcaagctccc aacaccgttt tacatatttt acaactgaaa aaaacagatt atatccatct
      841 catactttca ggtctccctt cctcctcttt atctcctcct tccacacatg gtatggccct
      901 cctcatcatc tacaccccca acacccccct ctctgtggtc ctgtcctctc ccagagttca
      961 aactaatgac tagtgacagt ccactgaggg gagcgagctg tttgcagtca gcagtgcgtt
     1021 tgttggtaat tattttccag agtgtcacat tagtgtcagc ctcgtggcgg tgagagagga
     1081 cgggggcccc gactggctgg gtgggtgaga cagagagcga ggggagggga aagggtgtga
     1141 tgtttggtgg tggccagcgt tgtgtttatg tgtgtatatg tgtgtatact gaagtggtgg
     1201 aggtgggttt acgacacagg ctgtcactgc actttactct ttgcctcgtg gctacgtaac
     1261 actccggttt ctgtcctgaa aagacacagg agcctttaat gtataaagaa taaagctggt
     1321 gatattctat atttttatta ctgtcaccat attttatgga gagtccaaaa ccaactataa
     1381 gtttgtcctt caggcaaatt tgtctgtgta gccaaagctt gatacaactt attcctcagt
     1441 ttcatctcaa tcttcttcag tctattagaa acacaaatat gccaaaatgt ggcaaacatg
     1501 gacactgcag ttaatttaaa gttagtccca cttacactgt cctgctgcca ttaatattca
     1561 catgcacacc aaatgtgtct aaatccacag ctgaaaatag tccccaacaa atgcactatt
     1621 tcctcctgtt tgttt
//
LOCUS       KCVB01000556             751 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26269pri ultra-conserved element locus
            uce-570, sequence.
ACCESSION   KCVB01000556
VERSION     KCVB01000556.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 751)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26269pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>751
                     /note="ultra-conserved element locus uce-570"
BASE COUNT          227 a          112 c          167 g          245 t
ORIGIN      
        1 ttttgaatag tgggtttctt ttataagata cagtcattta catagagttt taaaaagcaa
       61 tttgtgttcg tggtagtgaa ttagtttaag tgggttttgt ttaaatgggc agtggtatcc
      121 tcataaagag ttgctttgaa taaatgacat tatgtgcatt tgtttggact aatttgacac
      181 acacaaaact gtgcagagtt tcaagcttcc tcgctgtttg gttagttaat taacaggcag
      241 taaatagaga gggctttgaa acagcttgaa atgaacaata accttttaca gtgaacccag
      301 aattgcaaat ggaagtcatg gtcaagcctg tttgatccat cgctatgagc atctctgtca
      361 ttgctttcat agtttttatt cattggtttt tccattgact ggcttatgag gctctaatgc
      421 gacatgtaaa ttatctagga aaagtattct ggggttttgc actgatagga aatccgcgcg
      481 atgcctcaat gtgaggagtt aaatgttttg aaggtcgcag tttctctcat tgatcttgca
      541 atgtttgtta attacattgt cactgatgtt gtgagagata attaagccac tatgtttata
      601 tgcataatta cgtgcactcc gcatcagcct ttgaaagaca gcggcgagag agagagagag
      661 agagggagaa aaaaaatgtt ttaattgtga gctgagcaga aacatggcaa tatagatgcc
      721 ctagttacaa tcaacaaaga gcaactctaa a
//
LOCUS       KCVB01000557            1688 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26308pri ultra-conserved element locus
            uce-535, sequence.
ACCESSION   KCVB01000557
VERSION     KCVB01000557.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26308pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1688
                     /note="ultra-conserved element locus uce-535"
BASE COUNT          449 a          375 c          341 g          523 t
ORIGIN      
        1 acgacttttt tcccgtcttt tcttctctgc acagcgatcc aaaatagcag tgtatgagaa
       61 gatgtggtca tacatgaaat ccgctgagcc ctcagtgttt gttaagacca cacccgatgg
      121 cgtagcccgg gtacgaaagt caaagggcaa gttcgccttt ctcctcgagt ccaccatgaa
      181 cgagtacatc gagcagcgga agccgtgcga caccatgaaa gtgggcggga acctcgactc
      241 gaaaggctac ggtgtggcga cgcccaaagg ctcagcatta aggtgggtgg aataatataa
      301 caatattctc cttgttgtta tagtattcca cctaccctga tgtccccctg ttgtcattgt
      361 cttctcttcc tcttcttgtt tttttatgga cacagaggta acggtggtat gcctttcaat
      421 gcacttggat ataaaggtta aacaaagata atcaatgttt tttttaatat tttatatagt
      481 ttgtgacaag aattgctaat ttctagtaga ggaatcaaat ccagataata aggctaaagc
      541 taacggtcat ttcatattta tgtctctctt actgttgctt ttctctctcc ttcgatctct
      601 ccttctgtcc cgtgtgctgt cctacctgac tgttgtgttg tcacttttaa tttaacagtt
      661 caatggtttt tcaatttagc ctttaaaagc tgcgctgtca cttgtgacga atgttaattg
      721 catgatgttt gttgttgtta cttttcttct caatgacaag tgtccccatc ccgcacactt
      781 cagtttctaa actacgtaac ccttcatgcc accttttcca agatttaaca ctgtctttac
      841 cttatatgag cctttcttat ctctccatat tccttaaatt cttaaacaat ttgtcctttc
      901 ccagaccatt ctgattaacg gatagtactc tgtgcataag cccctgcttt gacagtggta
      961 gtctcactac cattacctat cattatgatg cttagtacaa ttataacttt gcattagatt
     1021 tctcacggac ctgtgcattt tcttacaagt tatgttttat cgtttcaaga aatgctgtta
     1081 acctggcagt tttaaaactg aatgagcaag gcctcttgga caaattgaaa aacaaatggt
     1141 ggtacgacaa gggagagtgc ggcagcgggg gaggtgactc caaggtcagc ctcgatgtca
     1201 ccacaatcgg gtaccatagt gcagagtaat cggcaaggct gttgcaataa cccaaccgta
     1261 accaaaccaa ctgtccaccc caaaccaacc tgctgctgcc ccacggcaga ttaccaaatt
     1321 aactgctagt ctgaccagac gttttaacag ctcacaccaa actaaggagc ctttgcttag
     1381 ccgacagaaa aacaacatta gtgatttttg aggctgtaat gttcacagac ggagcaaata
     1441 agagttagga tgggagtttt ttctctttat gacaaaagtt attggaagtt ctctaaaaaa
     1501 ggggaggctg ttagttggat gtggctgccc tgcagttgtg caaggttgag cttcttttac
     1561 tcacccttac acacatgtag tgcagatatt atattagaac tttgtctagg aagcttcgac
     1621 agtttgagta aaagcagttg atgaagtcgc agacatgtaa ctcgtgagga agcctccaag
     1681 tttgccag
//
LOCUS       KCVB01000558            1532 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26352pri ultra-conserved element locus
            uce-119, sequence.
ACCESSION   KCVB01000558
VERSION     KCVB01000558.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26352pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1532
                     /note="ultra-conserved element locus uce-119"
BASE COUNT          411 a          330 c          292 g          499 t
ORIGIN      
        1 agctatgggg ctgaacctct tcttctgcag gtaagacaat tagggagaga atgacaccat
       61 tagcagagtt acagcagcag gactttgtat gagcttgttt tatcatatca tatgagtaaa
      121 atgcctattg ttcaacttcc ctctctctct acaccctcct ctgtccttgc cctcggtatg
      181 aaaacacacc ctcctctctg tcagagcatc gttctcatcc aggtgtagtt aaccctttca
      241 cattccttac agactgagga cacccctgat acctcctctt tgtgctcaat ggagcagcca
      301 gcttagcaac taggctcaaa aggtttttga ttgcccaaat atttgtgtgc caccgtgtgt
      361 gtattatgta tgtgtgtgtg cttagattct tggagagagt gattcattta ggggcccctc
      421 tcctctcctc agctgctccc tgccttcccc agtaattagc ctagtgtggc agccacctct
      481 cgctgctcag tctgggagag acacttgaca cggcagacat cagagagatt tttcaggagg
      541 ctctcccggc gccccaacag ctcttaatta accacttagg tatccccact cgttcccccg
      601 gccggcactt tccaaatggt aaggaggttc tgtgctacgg atctaaacac cgggggaagc
      661 gtgctatcaa aagatctggg taaatgaagg cttgtgtaaa ttatttttaa tgattccctc
      721 ctggcacatc attatgtttg tagttacaca caggcacttc tgagacggct catcttcgct
      781 aaggtcatca gcaggaccaa ctgtaactag ctgctagtct gtaaatggct tattaattag
      841 tgctgtggtt ttacatagct gtgagctctg atatatagtg cagggatcgt aattgtgcgg
      901 aatcgaaaat gatgatgatg atgacgacga taacccatct ccaggctatt tatgcaaatt
      961 gccttctcat attagaaaat cattacctat ccaaacactg caacactcag cctgtgtgaa
     1021 ttgcaatgat ggattttatg ataattgcga tttaattttc tccctctccc tctctctctt
     1081 ttgtctgtct ggagtttgaa aataatttgg ccattgtgag tgctattaat tctgtattag
     1141 ttaaagctga acggatgggt ttattcaatt tcctctgaac tgagatttgt aaatcatcat
     1201 tttgtgaaac agaagatcac ttgttacttt ttttcactgg ctataattca gggatattta
     1261 aatagactgt taagccatta ggattattag ccattatcat gtcctttctt gatgactggt
     1321 tttgcccgct agtcataaac ctccactgta gttattcaaa tctgtaccaa cacctattat
     1381 tcgaaattta aacatgtatg agacttgaaa tatttcttat aaatatttta aagtattagt
     1441 ataaacagga tggataaaag aaaagtttta ttttatttaa cagtaaatgc cacaataatc
     1501 aattttacta aaatttacat tgttattata aa
//
LOCUS       KCVB01000559            1382 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26391pri ultra-conserved element locus
            uce-1292, sequence.
ACCESSION   KCVB01000559
VERSION     KCVB01000559.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26391pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1382
                     /note="ultra-conserved element locus uce-1292"
BASE COUNT          380 a          234 c          452 g          316 t
ORIGIN      
        1 ttctctatct gacctcatac tgaagctgaa tacctgcagc aatccatgaa gtggatattt
       61 agccaagctg gagtgtcagt agaaattttc tactatttgc aacatctgaa ttaaaagtaa
      121 aagattcact tattattgtt tcaacataac acaagcattc tcaaatgttt ctggcagtgt
      181 cccagcatta atgctcatct gtaacagggt cagagtgaaa attcaacaga catgatgaac
      241 tgacaaaagc aaagcacagc agagacccca gaaaatggag gaagaagaca ggtataaggg
      301 aaagattgcg agggagagaa agaagaaatg agcgtaggag aaggtgaatc tgatttctcc
      361 cggtaaattg gactcccctg ctttctctaa ttcatgtttc agagctactg catcgatggg
      421 gagatggaga gggtgaagag agagagggag aagaaaggag ggggaaaggg aagacaaatc
      481 catgcagggg agagcagagg gagagaggga gactgcggcc gctctcccac aggcagacag
      541 caatcaataa atcaatagga aactgtggtt ggctggagaa cggctggggc tgggaggatg
      601 gaggatggaa aagagagaga cctgaatcaa agtagctcca agatggatgg agcgaggaga
      661 ggaagggaga acaggaggag gaggaggagg aggaagggag ggagtgaggg ttcaatagag
      721 gtggaggcgt tgagaaggat gctggaagag gggaggagtg ctcggagaaa aggtggtttt
      781 agcaggagct gtgagggcaa aatgggtgga ggtcaagcag gcttttgggg tcccctccct
      841 gtcctgcctc cagcacatcc tgcatcagtg gtccagtgat agggcttggc actgtgcgat
      901 gccccgctgc ggtctgccca atgagcccag tgaagcgcgg ctgtatctgg ggctggggga
      961 aagccgtagg ccccctctgc ccgctgggtg cagcctggtt gactgactgc ttgcctggct
     1021 gcctgcctgc tggctgcctg gctggctgga catcgatccc agtgggttgg agagtacagc
     1081 tggacgcaca ctgagagctg gggctaaatc tggctcaggc tcaatcaacg tttgcacctg
     1141 tcttttccaa agggaattaa acgtagtaga tagagagaaa gaaagagtaa tggtgtgtgt
     1201 gagcatgtgt gtgtgtttat gtctgagtgt gtgtgtgtgt gtgttaaggt taagttttga
     1261 aggaagagta gaagtagaga gtttgcaagg aacatgctgg ttttgtcatt agtcaacatg
     1321 tttgtactgc agtaggtgag gtcatagttt atggttgttg tgtgtgtgtg tgtgtgtttt
     1381 tt
//
LOCUS       KCVB01000560            1754 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26426pri ultra-conserved element locus
            uce-671, sequence.
ACCESSION   KCVB01000560
VERSION     KCVB01000560.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26426pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1754
                     /note="ultra-conserved element locus uce-671"
BASE COUNT          510 a          402 c          334 g          508 t
ORIGIN      
        1 gctcttggcc tggttgtggg tgcaagcaac aggacaagcc tggattcttc tacagcttat
       61 ttacatttat ccttctcacc tattctttcc actaaagagc ttttactgtt aatgctcagt
      121 tattcaggtt atctggattt tccccattta cttcattaat ggcgaagcat tgtgtcattt
      181 atagcatata gattagaaat accacgttaa aaattataaa tcagacataa acaaaagcaa
      241 attgaaccaa attcagacaa aaaataaata aatccacaaa aaaaatatct ttcagtattg
      301 tgtagaaaac acaaaacgtc cacaataagc atataattgt aattcttata tcatactggc
      361 gatcaacact aacccttagt attaacattt ttttaatgtt tcataccatt gattgaacat
      421 tattgaaata atgtcccttg accacacctt gtccaagtct ttacacccaa acctttgaga
      481 ccctctgccc tgtctcttta acacacacgc tccccaaccc acacacatcc acacacacac
      541 gcagagggag agaaagagag agagatgttt cacagtgtct cactctgcag agaaaataag
      601 aaataaccat aactccttcc actgccagcc tgccctgcat gacccctgtt ttaagtgggt
      661 agcaggtaat tgctgaggca gtgttaccca tcagcctaca agtccatgtg tgccttatgt
      721 gtcatctcta tttattatat tctgcacgct ccctgggcct tggtaatcaa tttcacaagg
      781 atacagggct gatatcagct gccgtgcaca ttatgaggcg agccctcctc ttatcaagtg
      841 cagaattgcc ggctagaggt catgaacaga taaacaggga tgtaacacaa ctcccccact
      901 cacctgcccc ccagtgaaca caaaggtggg aaggtgatgt tgtgaggggg gtacaaagaa
      961 caaaacaccc agcaaggaaa aggttacgac ccttgtaagg tgcctcttgt tatgcccaac
     1021 cttcagaatt tcatcatcag tgcttctgtc tgcttaataa ttcggaagct cccgtctcat
     1081 ctctcttgag tcagcgtatg tgtgcgtatg tgtgtgtgcg cgtatgtttg tgtgtggtgg
     1141 tgatggtggt ggggttatga ggcctgcaga caccctctct ctgattatct ccaagcactc
     1201 tttgcagacc tttatagacc aaccacaact ccatcaatca tttgccctgc cttcttactc
     1261 cttgggctgc cccatgtccc acctcaccac tgctgggcta atattaccta aagctgtgtc
     1321 tgtagtggta tgcactgaat aaaaaaaaca aagtgatttc ttcccctgaa acttgggggt
     1381 ttaaaatata catatttata atgttttgag ggcacagaat cagatgttgc caaatcaacc
     1441 tcagtattgt ttctacaaat tattttttcc ccgacatcag atcgatttca ctctgaagtg
     1501 aggtattaga cagccacaat tgttgagaga attaagaatc tgtgtttaaa catgtttgca
     1561 gctgtagtgg agagacggct gactaaaaaa aaaagtgtta atagagatca tttgtggtca
     1621 gtggacaagt cgtgcagcac tagaaatgtc atgcagttgc atcagcttta gtaaacatta
     1681 attatgagtc catctttgtt tactcatccc tgcaccccac actggcagct gtgcatattt
     1741 gccaagagga ttgg
//
LOCUS       KCVB01000561            1393 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26540pri ultra-conserved element locus
            uce-321, sequence.
ACCESSION   KCVB01000561
VERSION     KCVB01000561.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26540pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1393
                     /note="ultra-conserved element locus uce-321"
BASE COUNT          388 a          283 c          265 g          457 t
ORIGIN      
        1 atgttcgcct tccaaagccc tggatttatt ttagctactc taaattgcta catttgtaaa
       61 acaattggat taacatcaac aaattaaaat tcatcttgat agtaccgcaa gtgctcgagc
      121 tgctccaaac agcggtttcg actttcctgg gacatctaaa tcccgcacag cgcagtgaga
      181 acacatgcag atgaaataat tagctaaagt ttataaatgt agacccataa tcttcttcac
      241 gtaattgtct gcagctggaa gactccagtg ctatgtgtga ttttcctcat ctcctcactg
      301 cacgatttcc aggcagagtg acaggcgatg tgctctccac atgtgaggtt tggttgtagg
      361 tttatcctgc tggccacttc acatgctctc gtgccctcct gtcatgatta acgagtgcat
      421 ttttttaggc tgtttaggtt tactaatagc agtttaaatc tatagaaaac caaaaatatc
      481 ttctctaccc atcctcctct gtgccttctt aacatgtgat accatatgtg cgggtttgtt
      541 tgcatcctct tctaaaaagc actggatttc acatattaac ctctttggac acctccatta
      601 atgagcgttt aaatgcgact ctatggtaag ggttgagctt taagtggcac agaatgaaaa
      661 atgatttgta ttgatgcaaa tcgtgtgtac ggagtgaatt atacacaatt acccatcaat
      721 actggccagt cttgcccacc ctcaccaaca agatgtggtt aacaggcaga aaataagatt
      781 gaaatggtgt gtaaagagtt gaaaatgaat ttcaatgctg catttgtcca caattacccc
      841 gtcgctcaca cgtgttttat tgggagtttt ttttttttct tcaaatattt ctcaaagcag
      901 attggaaaca ttcttggtat taccctcttt cttcagagaa gaaagaatta aggattttgt
      961 gacaaaaaag agattttctc tcctgctgga gcgacggagg ataacatgga ggctcacagc
     1021 gtgtttgatt gttaatcgtg gcacaggggc tttaactgct aatgctctca ctgcaaacct
     1081 gcagaaaaac accaggaaca tggagtcgct tttttttttt ttcattaatg gctctctcta
     1141 atactggcat ggggtactct gtcgctttct ccccctctct tcacaggcat tcaagaatgt
     1201 gttgggatat taaacatttc ctgtcatagt gaggattagg acaggagcct ttgggcaact
     1261 gtacagcatg gattttcact attcaattac tctatcaagt gggtttgatt aaagtcttta
     1321 taaaaaaaac cacctgaaat gcattttttt aaattcattt atttatttag aacttaaatt
     1381 gtggactttt ttt
//
LOCUS       KCVB01000562            1489 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26577pri ultra-conserved element locus
            uce-364, sequence.
ACCESSION   KCVB01000562
VERSION     KCVB01000562.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26577pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1489
                     /note="ultra-conserved element locus uce-364"
BASE COUNT          434 a          297 c          340 g          418 t
ORIGIN      
        1 gcaatttaga agtccataca cattcataaa agtcctactt attgattctt ttgctgctgt
       61 tcccacacag tctggcaatg tagagtttca attatcacta tgtgacctgc cattgtctgg
      121 gaaaaatgtg atctatagcc aggaaacctg aaagtttgca gaaaaaaaaa caaagaaaat
      181 ctgagagaca tcgacagcag cagcatgcat tcaataaaga gacgcttttg accttgtaga
      241 cgtaacgtaa aaattgcctg taactcttta gacatacaag tgatgtccag gctcccacac
      301 tgcagaaacc aaagctgaat ttcaaaataa atctaaactg gacaaaaaaa aaaaggacca
      361 gttcctgcat caactactca atgattcctt catgtaacta ttgtcaattt tgtctgcagg
      421 tatatataca gtccaggcaa cagagtcggg ccccctgatg gatacaggcc tactgggggg
      481 ttgcttattt agtctggcat gatgtgggct gtctctggct ccagaggctg cccctcttcc
      541 tgtttacctg cgagtccttt ggggtgaggt tgagctgaaa agcccagtcg gagccagtca
      601 aagggagcgt ggcgcagtct tgaccctggc gctccactca ttgtgtagtg cagcggtgta
      661 atgaggccct gtgtggatat tgatatgaca gaacaaaagg ccaaggtgca gtaaggacag
      721 caatccacac gtttcacacc atttgttttt cacaccgtat ccatcacatt aatgggtcac
      781 ctttctgtga cagctcatga ttacctgctt acccatggag agaaaagggg tgagagagag
      841 agagaaagag agagagagag gtaagtgcag agacacagag agagagagat aggcaggaag
      901 atagggagcg agggtgctcc ggtgctgagg ctaaagggtg agttgaggtt ggaagggggc
      961 tcagggagtg gggggatggg aaccttgcgc cattttttta ttctttcatg aagattggct
     1021 ccggccctgt catcatcccc acagagtgaa tttatgacag gtcagctctc catgtagtca
     1081 cctgatggat ggagaacatc caagtttatt tggggcatag tggaaatggc cgaacaattc
     1141 cctaaagcat caacagattt ttttattttt ttaagcaagg ggaagactca cattcaccaa
     1201 tagatttact aacaaaaatc attttcaatt gttaatattt tttctatttt gttttctcat
     1261 tttttaactt tatgaccttc ccaaagtgcc ttttttaatc gtcaagttta taaggaatcg
     1321 taagagcgta atgcatccta aatgcttgat aagtgaataa ttattgacaa taaaatacaa
     1381 gagagccaag ctttttctcc cattgatcac atatcttgat atgagctgtt gtgaaatatg
     1441 aatatttgct ctgtttttcc tccatctctc tgctgattat tgcccatgc
//
LOCUS       KCVB01000563            1612 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26623pri ultra-conserved element locus
            uce-748, sequence.
ACCESSION   KCVB01000563
VERSION     KCVB01000563.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26623pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1612
                     /note="ultra-conserved element locus uce-748"
BASE COUNT          453 a          312 c          346 g          501 t
ORIGIN      
        1 aggtctagga ttctttttca agttagtttc tgtattagtt gagacattgg tcaacggtaa
       61 gtaagtggga ctgattagac aacagcggtt tttatgcatg ttgctcaggt acaaggttca
      121 cggtaagagg gtcatcctac caacagcaaa tggactgcga tgccttagca tagtacaatg
      181 gtgtcggaca atttgttttg actgatttca actaattgta ctcaccttcc cctctccttc
      241 cctttgaact gcaaaggtaa gaagggaatc ggagaggtag tcttgaaaca gtactgacag
      301 atagagctgt gttctcactt tgaattatgg agcacacagt tggatgtcag aatgtatcgc
      361 ttttgagatc atacaactct aatatttacg aacagtatgg gtgtacttgt tgtgttgtgc
      421 atataaatta gcccgctttc catattaatt atgttatgtg atgtatttac attgatgtca
      481 gcataaaaag ttaaaaagta acaactattt attgtccgta cgaagaaaaa caaacaaaac
      541 aggagaatat tttgtctgct tgcattaatc tggttgcctg agtgtatgct taagtacata
      601 tagtcctact gtacatagag ctcgtgcatg tcatatactg atctgggtca catgtcaaga
      661 tggaggaagg acattgtcct ctggtatgtt ttgctgtgcc ctcttcctta atttgcctat
      721 tgtgagtgtg aaggggagcc agggaaatga catcatgcag caggcagtgt ttgaagtgcc
      781 agtcctgaag gaaccactta tagcaattag gatatccttg aacaagcgtt tgcgaaacat
      841 tggctggttt ttggcatttg cctcccgttc ctgcctacta actagcgagg tttgatctcc
      901 cataattatc tacccaccgg ctcctcactc ctgacagctc tctccctgga gagggctgaa
      961 tgctggggct actcactgat tgcatcaaag tgtcagaaat tgcaacatca aaaataataa
     1021 ctgatattcc ggcggcaggc tgctctttga atatttcctt taaaatgctc tctaatttgt
     1081 gtctcgcatg gtgccaatgt gtgtgtgact gtgagggctt aaagcaaata aaagcagggg
     1141 aagtgcattc attattgaac ctctatttac atatggcaga ctattctgtc tgaactggca
     1201 taaaatagat gtgcgttaat tctggtgctg ctttacgcca tcccgcattt ctttcaggac
     1261 ttgctcagca tctgtccctt ttgcctcctt cctcctgact ttgaaataat gctgcagtgc
     1321 cctacaatac atctctggca tcaacatctt aaatgtccca tcagcagaac taacaaaaag
     1381 tggcttggcg atgcagaggt aagagtgacc atcccacgac taaaagtctg catatttgtt
     1441 tcagactaca gtgtatgaga atgaatttta gcacagcgtt attgtaacta gaattaaata
     1501 gtttatttgt aaaaaaaaat gtgaatttag ggggttaaaa tagccaaata ttcatgtttg
     1561 tgtcttttca tgtcggccca ctggttaaat acagcagagt aaaaaaactt ga
//
LOCUS       KCVB01000564            1684 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26667pri ultra-conserved element locus
            uce-399, sequence.
ACCESSION   KCVB01000564
VERSION     KCVB01000564.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26667pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1684
                     /note="ultra-conserved element locus uce-399"
BASE COUNT          532 a          304 c          320 g          528 t
ORIGIN      
        1 gattattgac cggttatggt tgcctttcag ggattaagaa acaagaaaac tgtccaaact
       61 ctcttatgta ttttacaaag cataaaacaa ggtttttgtt ttccttactt cattaaaaac
      121 taacattttg aatggtcatt tcattcgaca aagaaagtga agtgcaagca tagatttgca
      181 aacatcctgt aggccaatgt gctgccagca acatcactac tatttcttta aaagttacta
      241 gttccccaat ggaaattaca atataatctt ttacaattat gaaaatgtag gatttcacac
      301 tattatataa cgaaataaag gaataaagcc atgaacttta atgttgcttt tttaaatatt
      361 tcttcattta cttcgattat tcttggtgtg ccttcctgta agtctatttt tgacagaaaa
      421 ctttcataac agacacactg aatctgaaag gagaacttta tttccaagaa aacttttgtg
      481 atgtggaaag gaagaaaaga aaaaggaaaa gtcatgcaag gcaaatatca cacagcgctg
      541 attcaccatg tgagcaaacg ctgagcccaa gttgagacta atgaagtctt taatttggtt
      601 atagtttatt aatgtcagag acactccccc attaatcatg ttataacgcc cctgagtgtg
      661 catgtgctcg cactaatgtg tgtatagcct gcatgtgtca ctttgcgggt gtgtttgagc
      721 atatgtctgt gtgtgtctat gggtgcttgt acacatgtat gagtgggtgt gtgtgtgtgt
      781 ctgaaagaga gagagaaaag tgtgtggctc tccactagca ataatatcgc atacatttta
      841 aaggaccgtc attatcgcca cgggttaccc cgcggccccc catcaccaac cccacaaacc
      901 attttcattc caaactcatt aatatgcaaa cttattcaaa tgagattgta attaagcatc
      961 tactgagtgg gaaccctttt catgataatt tatgataaac tcctcggcac ctttgattga
     1021 gattgatttt tggcgcaggc aaggcgacct gaatacaaag ctacacctga gagacctgga
     1081 gggagaataa cacttttaaa acagcaaact tatttcatgg ctgattgtgc cacaatcaac
     1141 ttggaggagt aacaaagatc tatttgaata atgcattccc actgtgctgg cgatgtgctc
     1201 agccttaaaa aaaaaaacaa aaaacaaaaa aaacttgggc atatctgttt ttggcatcaa
     1261 tgagacaaac tgatgagtgt ggatgtgtgt ttgtttgtgt gttttataaa ggcaggtaaa
     1321 tcaagcataa ctctattcac caagcatttt attgaaagga ttacaacctt ttttgactga
     1381 aattgacact ttatgaacat tgttatgttc atatagatga tctgttggcc tcatctcatg
     1441 cccatatttt cccttatttc accaattata ttttcctgtt aaattgcaca ttgcatgtgc
     1501 acataacaaa ttgttggtcc agcacaaaac atctgagaaa ggtttcccac cattaaagga
     1561 ttacgtgagg gagacatctc acagtggtgt ttttgcatgg ttttgcttct tggaaaggtg
     1621 ccagggattt ttattgctct tgtaaaaagt tgggagactc ataagatacc aattttaaaa
     1681 agag
//
LOCUS       KCVB01000565            1454 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26701pri ultra-conserved element locus
            uce-1057, sequence.
ACCESSION   KCVB01000565
VERSION     KCVB01000565.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26701pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1454
                     /note="ultra-conserved element locus uce-1057"
BASE COUNT          434 a          336 c          312 g          372 t
ORIGIN      
        1 acacacacac acacacacac acacacacac acacacacac acacacacac acacacacac
       61 acacacacac acacacagct cttctaaatg gccacacaat aaaaaggggt ccgtgaccaa
      121 actggagtgg gtagtgacct taagtttggg aaatgctggg ttacaccatc ttaaattatg
      181 gagctttcaa attttacaaa tttagtagca tgatattatt ttgagacact ggggtagttt
      241 tctgcatgtt ttttcttgct cagggggagg gtcctaaagg atgctgggca gggcctcact
      301 tttttataaa ttgaaacaca aagttcaccc cccctcccaa ctccaagaat ttatgtacag
      361 tccctaagtc agtgaaaggg aggggggggt gattgttcag ctgagtaaag tcatttctaa
      421 tttttcctac ttttcctcca taaagtgaac ttgttgaaat gtcctctttg tccatggcag
      481 ctctttggta aaacaatcca gggcaaagtc agagcgtgtg gtcgttaact caggctggca
      541 tacctgtctc gcttttatag gtggcaaaag ctactaatcc ttcagtccaa tatttactta
      601 gtccaagtta tgctttcctg gaccctagct atgcatcttt cctgctccct gcttatatat
      661 tcttggccct tgatgtattc ctggctcagg aaacaacttt tagtgcccca caagggctga
      721 gtgagaattg acagtgtgtg ccagagtgag agcgagaggt agggggcctg ttttagttcc
      781 actgtgtttg taaggtcact cagagtacat ctgtatggag tgggaactca ctcgctttca
      841 ctgtcacaac cactcacaac agccatctgt aatgctgtta tgtggagcac tgtgtccctt
      901 tgtcccttac acacacacac acacgcgcgc gcaaccacac agtcatcaca caccctcaca
      961 cgtataagag cacatatatg tgcacgcagg catgcacaca ctcacacaca agcagactca
     1021 aacccgcaca cactcatatg gcgtcaccgt tcacacgaca acaaatgaac cggcgcaaac
     1081 acacattaac ataatgctag gttctaattg cgagagcatg tgtttctaat gaaaaagttg
     1141 ttttagggtt ttattccgct cgactgtcaa acaattcacc catgttttga aatataattg
     1201 tggggactgt aagagtttcg aaaccaaacc aaaacattgc cgcgatcctg gtttctaatg
     1261 tttctaattt gttaaaatat gctgagtgaa tgacaaaagt agaagtcgga cctgccaaga
     1321 aaaacatatc agaggcaggg ccgtgtggaa atacagagtc tcttttagaa acatgatcaa
     1381 tgtaaccttt gttacaggaa ggagagggag agagagagag agagggagag aaagagagag
     1441 agagagggag agca
//
LOCUS       KCVB01000566            1580 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26744pri ultra-conserved element locus
            uce-24, sequence.
ACCESSION   KCVB01000566
VERSION     KCVB01000566.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26744pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1580
                     /note="ultra-conserved element locus uce-24"
BASE COUNT          491 a          301 c          313 g          475 t
ORIGIN      
        1 aagatcctca gaataacaag aacatttaaa gcccctaaga ctgatttgtt atcattaatt
       61 tcactcttaa tgtagtaatt ccattgacaa aatagacttt taacaaatta tttagcaagg
      121 atgttaatgt tagtaataca tatatgtgta aagtttgggg accctggcat gtaaaagact
      181 gggaattttt gcttcctcca accatacaca tacaggtggg atgtacatat catagttgtg
      241 gtctattaaa atatacatct tcctaaacat gataaataga aacttaaaac attatagctg
      301 atttacatgt tcctggatat aaagactaga ctcaaacatc aaactttttt atttatcaat
      361 ttatgtattt atctaaatat ataataaaca aatagggtga ttttataatt cgtgcatcgt
      421 gataaataaa gacacgtgcc accaaaccat cttccctggg ctctttggct tccaaacagg
      481 tctctgggtt atattgtgtt attatatctt tccattaaag cagtttaaaa taaaccaaaa
      541 agtcgaattt aaacttacag ttttatttac tataccatag tcctactaac ggccatattt
      601 ttactaccct acatgagaac tcatatagag agcgtcacac gggcacgacg gggtcagtga
      661 ccgcgcacgc cgcctgttta tctgtatgag aggtcctcgg gagcgcgccc ccccctggcc
      721 gaggaaggtg agcgagtgtc tggaggggag gactcgggag cgcgaccagc cgagggatcg
      781 atcggattaa actcgatttg agtgaaatta acacaaagga gagaagagca acgcacattg
      841 gtcggcgagg tcgtggatgc aagacacgcc ggagaattat ttcatgaaaa acaaacttga
      901 acttttttcc tctgtttatt tatatttcta acgaaaaaag gaccaaggtg gatttttttt
      961 caagcaagcg gagatactaa cgaggagttg atttgataaa aagcccgaag tgaccgagct
     1021 tgttgggtaa gctaacaaat gcccctttcg gatgagtgca ttttaaatat tttagagcag
     1081 aattcagtac atgcggtggt ttcataataa ctactaacta cgaattaaaa ccgtttcact
     1141 atattctttc ttaatagtaa gttttccata cttttgcaag gcaatcggac tcttaaatcc
     1201 ggcgtgcgag ttgggctttc agcccgatgg gcatttcatt cagtaataac tcactggaga
     1261 gcttgggttc agaactatag ggttatttag cttgtatttt attttctcat ttcattagaa
     1321 agtcattgaa ggttcatgta acgttagctg ccatgccgac atgctaacaa tagcaagttt
     1381 agctttgcca cccgctgcat cgctcataca cacgcacaac agtcccatta gttagttgaa
     1441 agtggaaaga aaaaattcca gacagcacgg agaccttccc cgtttcagag tcaggttccc
     1501 ggacaacttg ttgaattttc taatttgaaa tatagactta tctcagtttt aaagtgtgag
     1561 tgttagccca gttagctaat
//
LOCUS       KCVB01000567            1634 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26783pri ultra-conserved element locus
            uce-826, sequence.
ACCESSION   KCVB01000567
VERSION     KCVB01000567.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26783pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1634
                     /note="ultra-conserved element locus uce-826"
BASE COUNT          478 a          350 c          328 g          478 t
ORIGIN      
        1 tgtatattat ggggcactga gaatactcaa gttgacagtt cgacacttct atgaaattat
       61 ttgtaaacac agaaggtggt tctgcgtaaa tgtagcctgt agaggctccc atatgaccct
      121 gtaactctac atcttgtgta tatttgttgt tccatgaaaa agccctgttt gcttcagctg
      181 gaaaggatat ctagagaaat ttattttatt ctttgattta actttattaa gtaaccttat
      241 tttcttgcaa tgaagttcct attctcaagt ttttcttcat ttcctgtata ttctggttga
      301 agtaaatgca gccctgcccc ctagtgcaca cattaatgtt atgtgtatga taacagacac
      361 ctatggaaat ctctgcacat gttgtgtgtt attgtttata gacttacagg cacagaggca
      421 tgtcccaggc ttaacacagc atttgtggga taagtaagag cccaggcaaa aaacagaaga
      481 aaaaaaacaa cagttcttcc tttcaacccc cacacacctt gctctttgct ccgctggaga
      541 aaaaagggga ataaaataga gtgaccctgc cactagcagc tctctttttc ccccactgct
      601 ctcttcttcc tcccccttgc cgtgtgaaat caaatttcca tgtggctccc gtatctgttg
      661 cacagcgagg tgatttatca gggatgcaaa gagcatgcag aaacaatctt tattacatcc
      721 atttcctgct tgtcagccgg gatcatgttg gctgttcatt aaaggcgcgg cgcaggtgag
      781 gagaatgcat cccgatgcct tgatagattg tctgctcccc tgtggtgaca gcagctggat
      841 ggattgaaca gcggggggtg aggagggagg aaaaaaagcg ctgactccgt cgctgcccct
      901 gtcagacaca ctttttacat agccggccag tgtcatttct agcaaacagc ccctcacaca
      961 catgcacacg cagacttctg ccactctttg gacttaagcc aggagcctga cttaatgcat
     1021 ggatgcatac aaacacaccg tgagcataca aacacaagta ttcatagaca ctgatgtaaa
     1081 aatacagaca cgttgttaag atgaggttta cagttaccaa ccttagctct ctgttgaaaa
     1141 caccagtctg ataaccgcca aagaagatga catgcataac attgactgtg acagactttt
     1201 tgccattttc cccccaaaaa atttgcaaaa taattgtatt aattttaata actaaacatt
     1261 acactcatat aagtaatttg gtgttgcttg gagaccacat tgtttagcta cctgtcaagg
     1321 accttgttgg atgatggtgg aagataatca tgataatcat gctcttatta ttttgtgcaa
     1381 aggagcatct caacatcagc ctgctatgtg gtttatgtgg ctcagttaac acatactgta
     1441 caaaaactag cataccagca gtaagcctga gcagttgttt cattataaat ccattaaaag
     1501 aaaaggctgt tctctttaca taaaacttga actactgctt ttagatgcag tggtatgttt
     1561 gtggggctcc ataaaatcaa taagtcacac aagaccagct gtttagaaat cactgaggaa
     1621 tacggtgagt tagt
//
LOCUS       KCVB01000568             802 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26853pri ultra-conserved element locus
            uce-655, sequence.
ACCESSION   KCVB01000568
VERSION     KCVB01000568.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 802)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26853pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>802
                     /note="ultra-conserved element locus uce-655"
BASE COUNT          253 a          114 c          225 g          210 t
ORIGIN      
        1 gggggggggg gggggggggg gggggggggg gggggggggg gggggggtac agcggagata
       61 aatcattaga gagcaggtta ttggaaatgc agggagataa aagagtgaag aagccagtaa
      121 ttgccttaaa gatcagacta caaagcatta ctttggattt tacaaggcca gccagcaaca
      181 aaagagcagt aatttatgag ctcagtgtaa aagaatagca taattacatt aaaaattaat
      241 gctaatgagg gctaaaatgg ggaaggtggt tattatgaaa gtttgtattc atcttccctg
      301 gcttgaatga tggctagtgg cttgattaat ggcttaattt ctatcaatta gcaggcaaaa
      361 aaatctccac tgagacgggc ctaaattgag cattgaatta cacatcttta ccgtggtgct
      421 gggagagagg tagggggagc agtggaggga agtgcacatt attgaaacct gggaaaccaa
      481 gagatgttgc tgtgtgtgag ggggtgcatg cacaaggaaa agtctggcat gggttacaaa
      541 acttgaacaa atgtttatcc tcatgcactg gggtttgtat cactgctggg tttgcagtgc
      601 agtagactgt gtcagtgctg tgatgcttgg agaaacacat agagagggcc gctgaataaa
      661 tatataatat ttaaaacaaa tctttgtgaa tttgaatctc tcttaaaaac attatattct
      721 ttgattaaac tggcctaaat tcaaattttt gtacttaatt tagctgaagt ccaactacac
      781 accattaaat acagcagcac at
//
LOCUS       KCVB01000569            1303 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26892pri ultra-conserved element locus
            uce-554, sequence.
ACCESSION   KCVB01000569
VERSION     KCVB01000569.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26892pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1303
                     /note="ultra-conserved element locus uce-554"
BASE COUNT          349 a          259 c          285 g          410 t
ORIGIN      
        1 atcaaatcga tatcatcctt cttacagctt ctgtgtctga ttactattta taatctgggg
       61 ttgtttaatt tttggcagct gatttgcaaa tgacaagtgc actcacaact gatcaatgta
      121 cctctgacga ataactgatg ctaaattata actgtgctga catgaaatca acctcataat
      181 ctatgctatt atttatagaa ttgacagcag taatacataa gcaacttgtg tttaatgggc
      241 tacaacacac ataatctcta ctttggtcat ttgaacgctg tgtctaacat gtgacactac
      301 ttttgtgttt ctgcttgggt ttctacttta tctgcactca gaggcagttg gtatgaatgt
      361 gtgccactgt agctgtagat caatacagta caatcttctt aacgggcata gagctttgct
      421 cattttgcca agtctccttt ctgcactgca tgcctgcggc gttttgctgc caggcctcct
      481 ttcagttaga cctctgtgtt tgtttgactc tctctgacaa caaggtcagt ggcattgccc
      541 agtaattgtc ctaacggcct gtatacctgt tgggtggagg cgagtaatga gagtttttca
      601 ttacgggcgg gacgggtgag tgcgcctggc agatgctgac aggcagcaga tgtgtaaagt
      661 tgggggtgat ttgtaaaggg gtcaactgca tcacagagga gatggagggc gatgtggggt
      721 gggggtaaat tatactgagg gacattgtca gtcatactgc gcccctgtca caacaaggta
      781 aaggtcagtt caaaccttga tgttgtcatc caacagccag cacagtgtgg tcatgtcagc
      841 aaagtgcaca aactttctac cacaaactga ccgaagatgt ttagttcaaa gtttatttca
      901 tttctttgag tcctgttcca gtttgttttc tcccttcccc ctttgtagca tgtgccaaaa
      961 aaaatctgtg ctgatgtttt taaatacttg aaactactcg tgacatgatc agaaaaacca
     1021 tacatgtgct ccttaaaaac ttgaaatttt gtcttgcgtt aatgcaaaag ttaagggaag
     1081 ggaaaagaaa gtgcaaagct ttcgtccttt tctgtttcca tgtcattgtt gtgcattgag
     1141 tctgtgcaaa aggaaaacag ggatttgttc tgaatcctgt cttattaatg acacacacaa
     1201 catgcatttg tttcctcagg cattcacatg gctttgcagc tacacatgaa ttcagaaatc
     1261 aatgaaaacc atgatcctca gttggacaaa gatgtggatg atg
//
LOCUS       KCVB01000570            1289 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26933pri ultra-conserved element locus
            uce-185, sequence.
ACCESSION   KCVB01000570
VERSION     KCVB01000570.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26933pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1289
                     /note="ultra-conserved element locus uce-185"
BASE COUNT          318 a          266 c          258 g          447 t
ORIGIN      
        1 tgctttctga cgatgaaatt tccgccgaag aggtattttc actgttgtgg tgcgtaccga
       61 aaagactgtc aatttcaaat ttgcctttcg tcgggatgtc cccaagattt ccatggagag
      121 aagccgaagt tattctcggg cttagccgtc cgctgtgctg ggagttttcc agggcctcca
      181 gcactgaatt tcctggcgtg tctgcgagcc tcttccctgc gacgggactg tgcagacctc
      241 tctccatcaa tatcatctct tttcttatcc tctccatcat taagccgcgc acgacgaagg
      301 gaaaaaaaaa tcacacacac acacacacac cacttgtcca gggggctgga gcgccaatga
      361 gttgtgtcgg agcaaaatcc gcacgcaact caacaactgt ctctaaataa ctgtctctaa
      421 agctttttat aagcaagtcg caaaaatgaa agaatctgaa tgcaatcgac gagcgactgc
      481 tcaaaatgag ccgtgcatcc tccccacagc agcaaaatgt cattcatcaa aagtggctgc
      541 tgttcgttgt taaaatgctc ggctgacgtt gctccaatga tcatttattg taacaggttt
      601 ataagcaaat aaataggaga gggctgtcac ttgatgatgg aggcggcctt cggtcagacg
      661 aataatatcc aagtggagag gaagagagaa gtgaggagcg aggtgcttgt ccctgggttg
      721 atctctgagt ctgttttaga ttgctcttgg gtttggcctc ttgggtgaaa ttgacagtct
      781 gattaataaa atgagcggtg caacatttcc ctcttcattt aggaatatta ttatgctttg
      841 attctctatt gtttccctct tctctgttcc ttctctctct ctaatcgttg ttcttttatt
      901 tattctcctt atttcttttc tgcttattta tttgtttacc atttctgcga cttcatcagt
      961 atgtgtagtg cattaaagga ttaaggtgtg cgtttgtgat aggagattaa aggtattatt
     1021 ttttctcgtt tttaagactt tgttgatttt cttttagtgt tgtttatatt ttttcttcct
     1081 ttttttctat ttttttgttc cacagttctt tattaatcaa agacgtattt tgatgtggtg
     1141 ataatgcttg cattattaca catttaccga cttcgccaca cacttattta tttattaatg
     1201 catacagaag tagtctattt gttcgttcag ataacctagg ctgtattttt aaaatatcta
     1261 aaacgtcact aatcgttttt taactcaac
//
LOCUS       KCVB01000571            1321 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 26974pri ultra-conserved element locus
            uce-511, sequence.
ACCESSION   KCVB01000571
VERSION     KCVB01000571.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="26974pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1321
                     /note="ultra-conserved element locus uce-511"
BASE COUNT          393 a          225 c          308 g          395 t
ORIGIN      
        1 caagtgatgc tgtgagatca gctcgcacca ccgtgatgct gttgtacatc gaagtcacat
       61 atttaatgac ttgtgtttgt ctttatattg tgacgaaagg ggaacccatg ttagtttatg
      121 ttattgcgtc taaaagcgaa gaatcttagc gttccttcag agcaggaagg tttcgtaaag
      181 taagaaaaaa aaaaagggca ggggggtgaa atgattagtg cgcttagtca gctgtgaaat
      241 tgagtttatg gttttcaaga cggtgaattc aattcgataa tgggtattaa gttgtagaga
      301 ataattaata gtaattgact tctttccatc actatgcaaa ttaaacacag ataacaccac
      361 gggagctttt taaggccagt caagtttgta gggattaagt ttatggaatt tttgttggag
      421 agaggagtgg ctcctgttct gctttgattg ctttgttgac agatttatca gtgggtgagg
      481 ggttactgtc tctgcaagga ttaaaggaat tgggttgtca caaactgtca aggcattgag
      541 ttaggagcat tctggtatgt ttgtcataat gggctcaggc tgttccccgc cgcactgtca
      601 aatacattct tattgaactg catctgtaaa tgggagagag gaggggaaaa tgcaaaggaa
      661 ggattgaatg gatagtgctg agggagaaat atccagcggg actgtgtgcg ggtggatttg
      721 tcaagatgca gatttggatt tgggaagctt gtgttcacac tgacatttgt taattcacaa
      781 agatattagt gagtccgtgc aaggagcagc tgcaaatctg gtttgtggtg gtgagatttg
      841 tcattaaagg actcaaatgg aaggaaaaca atttaaaaat gtgcctttaa ctccactata
      901 acacatgtta aatgagtctg aataagaaat tacatttttc tcaacatttt taagactttc
      961 acattatctg gctgcatctg atgtgatctt gcagcaataa tctgactaaa atcttgattg
     1021 aaagcttgat ttcacactat taggtcctgt tgtccttcca ctacctgaaa ctcactcaag
     1081 aacatttctg tttgcgaacg ttcctcgaca ttcctgctac cagacaacat taatcacaga
     1141 aatcgcagag atgtaaggtt acatgaaaat taacataaaa tcaaattgac atttaactca
     1201 ccagagagat gactgacagc agcagagcgg cggcccacag acatgtggtc ttcatgtctc
     1261 tctgtggagc gtctcccctc atgtcaaact gtccttgatc ttctcagccg cacaaaaaaa
     1321 a
//
LOCUS       KCVB01000572            1324 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27016pri ultra-conserved element locus
            uce-983, sequence.
ACCESSION   KCVB01000572
VERSION     KCVB01000572.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27016pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1324
                     /note="ultra-conserved element locus uce-983"
BASE COUNT          310 a          318 c          329 g          367 t
ORIGIN      
        1 agtagataca ggtttggtgt gctgtcttca aacttagaag catgtcctcg ataatcccag
       61 aatactctgc aagcaatgta ttatttatga ttgaactaca catacatctg tgcatgtttg
      121 gctgcatcgc tcatgtgttc cctgcatggg taagtatgtt tgtgtgttta ttcgtgactc
      181 acttacatct gtcacgttgt ccatctagtg gccatgatcg agctgcatga gcttgaagag
      241 agccaggtca gcgtggagtc ggtgcgaggc tggtgtgttg aggagcaggc gatggagatg
      301 gacattgctg tgctgcagca ggtggaggaa ctggagagga aggttactgc cgccagcctg
      361 caggtcaagg taaaatatta acaaaagaac acaaataccc cctgcacatt ttgccagtgg
      421 tgaaaaaaat gtgtttaaac ttgtggactg gtagaagccg aaagatcgaa cagctgattt
      481 ttcatctacc agggctggac atatccggac cctcagtctg agcgagagga cctggtgtat
      541 tatgagcaca agccccccac caaatcaacg ccagcgtcag caaacgacaa ggactctaag
      601 gaccatccgg aggagcgtgg ggagaagggt ggggtgatgc gtcacccgga caacccgctg
      661 gacatagcag tgacacgtct ggctgatctg gaacgcaaca tcgagagaag gtacctgagg
      721 agccccttag gtaccaccat tcagatcagg ctggataatg tgggtacggt cactgtccct
      781 gcccccgccc catccactag tgctgacagg gaagggtagg tcatttctcc aaccaaagct
      841 ccccgctctc ccactgagac cctttcctct ctctgtgtgt gctttatgtt tcccatatta
      901 cttaaaaagt gagggtcttt gctgctttca cagcagattt ttgtggtggc ttgttgcatt
      961 tttgtttatt tctttggtct tgtgcagtct tttgggtggg gtggggactc cacttttttt
     1021 ttctgcagtc atcagcatgg tgttgtgcat cagtaacctt gattatgggc atactcatgg
     1081 ctcccactat acagcataca cttccacagg ttctactcct tcactctttc tgcatgaacg
     1141 tccccttaac ataacaaaat cggtgtgtac ttctccctta agcccaacac ccctaaaatc
     1201 ctcatgccag tctctgcaca gctggagctc tttatggtct gctgtcttgt tggcggctgg
     1261 tacaactgat acagttcact gcttttgtgt gtaaatacgc ccacatattc acgtatatgt
     1321 gtgc
//
LOCUS       KCVB01000573             692 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27060pri ultra-conserved element locus
            uce-1233, sequence.
ACCESSION   KCVB01000573
VERSION     KCVB01000573.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 692)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27060pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>692
                     /note="ultra-conserved element locus uce-1233"
BASE COUNT          197 a          126 c          147 g          222 t
ORIGIN      
        1 ttaaagcctg tttttttttt ttttgacgtc ctgccttctg atcatttaca tcaagttgac
       61 attcggagag tttactctgg ggtctgtaat tgtattcttt tgccagttct gcattgccaa
      121 ccgtgaaaat ggaatcttat caaccctgac caatttttaa tgtacaaaac gaattggcag
      181 gggttcaaaa aagggggagc tacaattcaa atcaggtgaa tgatacacaa caattttttt
      241 cagcgaatgg ttgagctgcc cccgttcaca cacagcacca ccttaacccc caccccccac
      301 ctatcaaatt acaacagatt tatttttctt tcaaagcaat ggcgagtgta agaggggcaa
      361 cacaaggtaa aatcatatgc atgtataggt ttctgtgtgt gtattcagta ttctttgtat
      421 gtatgtggat attatgtata taccagtgtc tattctgtat gtatacaagg tttttgtgtc
      481 aatgctgtgg gggttagggg atgtttgaaa aaaaaagtgt caacaaaatg gcacaagggg
      541 tcaacttgcc acacattcgt ggtcacctct gaggtaagtg agctaattaa actgatgacc
      601 tacaacaaaa aagtgcagcc actgggagaa gttgccttaa cgttgttttt ccttttaaat
      661 ttgggttggg tgtgaatttt gaaatgacat gg
//
LOCUS       KCVB01000574            1767 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27104pri ultra-conserved element locus
            uce-1276, sequence.
ACCESSION   KCVB01000574
VERSION     KCVB01000574.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27104pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1767
                     /note="ultra-conserved element locus uce-1276"
BASE COUNT          569 a          312 c          409 g          477 t
ORIGIN      
        1 tatatggaaa aaaactaaaa gatatgaaaa aagaaaggat aatgaaaaga ggttatgaga
       61 aaccagggtg aatattattg ccggacttca ccaagatgca gagggaagaa aaaagctgtg
      121 caaatagtca tgggattgca aagtcaataa cgcgtgcaat cttcacagac agaaaactat
      181 aatcaacaaa aatccatatt gagggtgaat gccagagttt ttcaaaatgt cagtggagtg
      241 tgtttgcaga agggcaggaa tgacagatga agagcaggga ggtgagtggc agggacacaa
      301 ggacaggctg actatgtgct gatgaggtcc tggacacaga gaccaggcaa agtgcagcgc
      361 taggccacga gagcactctg cctaagcctg ctccctacag aaaagccaag aggagtcagc
      421 agcgctactg caatctaatg ggaaaattca taggttaaca agacagtagg cagcctggat
      481 tgcgaaatgg tagcaagagt gatttgtgga gacactttat atctacaatt ttagcaatga
      541 taaaaacact ttttcttttc ctgatacagt cctataaaat acaatgctat gtactacctg
      601 gcaaaacagc aactgaaaac tgggtgattg tggaggaagc gtggtttttg tgaatataag
      661 cacttaagtt gagtcatttt acagatatag atattttcca ttttcaactc tgacaaaaat
      721 gcagtaaaac ggtggggcca tttttttctt ttcaattttt gtctcgtaaa tccttttcaa
      781 acgcaattcc tattcttctt ttgaggtctt ttaagtcatt ataaatgttt tctctctccc
      841 tctctctctc cctttcccta gctttagtct tcttgaataa aataagtgca gatttgctgg
      901 aaatcagtgg cttttgtgtg tggctgcagg agcatggtga aatgtttgga cactaatacc
      961 cattcatttc ggtagcgcca taaataaaag ctgccagcta tgcggctgct gtgaagtggt
     1021 attctttggt gcccagcagg atgtcctgtt cacattagcg cagcagggaa aactggccgg
     1081 aacagccaga caactaaatc aagacattgt caacttctga caaaattgct ataggggggg
     1141 aaaactggtt ttaagagagg ttgtgcatgt ttggttgtgt gcatgttgca gtgtgtgtct
     1201 gtgtttctgt gtgtgtttgc aagacacaga gagaaacaga gagagagaga gcaagagaat
     1261 gagagaacaa gagaggcgtg ggggagatat cttgtcaaaa tgcagtgtat tctgaaaaat
     1321 aattccaaac agagcaacac catggggctg agatgaatga gaaagtgttg ctcgaatgga
     1381 ggcgacagtg cttagatcaa gaaataaaac ctaagcctcc ataaacactc atctccgatg
     1441 ttttgtcccc accaatgcaa tccaaaatgc ttctaaggat caattttcca atatgtacca
     1501 atgttgtgca agcttcaaaa tttaaaaaag atagaaagaa aaacactgca atgtgttttg
     1561 agttggtcat tgagcttgga ttttgacttg tttttggatg acagtgctct ggcctccagt
     1621 cattgtgagc cttctaataa acaacggaac ctaatttcct gtttgtaggt ataaatttta
     1681 ctaattccct caaccaaaaa caaaaagatc agaggtaaat ctctgggcat ctgtgataaa
     1741 cttaaaccag gttagggcaa acagcac
//
LOCUS       KCVB01000575            1103 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27143pri ultra-conserved element locus
            uce-569, sequence.
ACCESSION   KCVB01000575
VERSION     KCVB01000575.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27143pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1103
                     /note="ultra-conserved element locus uce-569"
BASE COUNT          316 a          228 c          248 g          311 t
ORIGIN      
        1 ttcctgtctt aaacctcctg caaattattg atttcctgga ggatggcatg tttaagtaaa
       61 agcgctgtga agacttgtgc tccactcagg actactctta ttgaattacg ggtcagagtt
      121 tactgttgtc acttgctcga aggcgaatga aaagctaaaa gtagctgctg agcttgtatg
      181 gggcacctcc acagagcaca gataaacact tcactggcag tttttttttt atatcatttt
      241 acaatggtgc gcctatggaa gtggtaggga gttatcacct agctgtcact ggctagaaat
      301 atctgagaag aacactttgg aatctgcagt gactatgttg aatgttgtat ttgtgggttt
      361 tagagcctag gcaacctctt taaatgccct cttgtactct ctgaggttct gttgtcctgg
      421 ctagatgcta cctgccatgg tgtgaggata aactcatgtg gtgtgcggtc caccaaacaa
      481 acctggggca tcttgggata gcgggagaaa gattggtaga aaacccattt ctgaagtgga
      541 tttgtgccct tgtaatcctg attaattttg acccgttgtg ctcattttaa acagagcatt
      601 acagtaagcg tgaacacagc aagctccagg gcgaaaccag gagcttagcg gctttgggag
      661 gcttagctgc agatggcatt ttaagccgga cttaataaat tgtttccatt aaacttaccg
      721 gcccacacat actggattag accagttgaa caaaggaagg agagagcgtg caaacgaaga
      781 agagaaaaat agcacacaca cacaaacaca actcccatca tgcacatctg actccgttaa
      841 catctctctg aggccacatg aaatactgcg gtgttcaggg agatagaagc ttttggacat
      901 gaatgcaaaa tccactcact ttagttctct tgctcgtcag ggatgtcgga ccaatttgct
      961 taattgccac agaagtaaac aaacaaaaaa gattcataaa ccatttagct tggcagtgga
     1021 tttaaggctt atcctctaac tgtttatata taaacacatt accagtgctg tagcttaagg
     1081 atttaggatc tgcccatacc ctt
//
LOCUS       KCVB01000576            1945 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27215pri ultra-conserved element locus
            uce-695, sequence.
ACCESSION   KCVB01000576
VERSION     KCVB01000576.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27215pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1945
                     /note="ultra-conserved element locus uce-695"
BASE COUNT          615 a          383 c          451 g          496 t
ORIGIN      
        1 atttataaaa catatgtact ttcttgatgc ttttctctcc taatatgatt cctattcctc
       61 agctcagcac aaagggacag ttttcaggca ttgccagagt tttatgactc ctctttcttt
      121 ttgcctgagg cctttaacct gaaggaatac agccatctga ccatttcact gttaaactgt
      181 aaaagctagc tagtgacatt tagagagtgg acaaaaacag aaaaaggaga gcagcttctg
      241 gtctaaaaac aacactttgg atgggagctt aaataagaaa acaaatagag actggacagg
      301 tggaggggac acaaatcgag cagttcacta gagagagtgt gaaaaattgt ggtgaagatc
      361 tgtcaagaaa agctcgacat ctcgggggga gaaaatatca gattttgtga gaggagacca
      421 cgtatctcag ctgtaaggcc tgaaagtcct ctccctctgg cagtccctgt cccagagaga
      481 cttcttattc ctaaatcttt ctaccctgtt agaaacaaac attatacata tagaaaatga
      541 gagagaaaac ctacagtgaa ttataaaaca aatgatggct gttaagtttt cagaccatga
      601 gagaaacatg atagaagcaa tgcagttagt catgctgttg aagagaggag agagagagtc
      661 agtcaaagag aggcagagag agtgagagaa aacggggccc attttaagag ggcacactat
      721 gttcggcccc ctgtctctta atcctgggcg tttgtcgcag ctaattatgg tggacaagcc
      781 tctttatcga acctgctcct gccatcggca ttaggatgca tgagctgcgc ctgttgtttt
      841 aaacgctctc attaaccaca caaatcagtg ctgttgacag agtgcttttg aattaagctt
      901 ctcgccccca ccactgcgcg ccaatgcgtg cagggcccag gacaaggagc aatttataac
      961 accattagcc acactcgccg aggttacagg catcaaaatt gttcccgtct aattattctt
     1021 aatggataca gggagggagc gacgctcagg acagcttttt cattagccct caatcaagag
     1081 ggtcggggga gagaggggtg ggccaaatgt actgctcagg caaacgggaa atcaaaaata
     1141 atcgtgtttg cttttttcat aatacaacac gaacacatag atggaaggag agagagggat
     1201 ggagaagggt gagtgaggag atgcagagag aaaaaaaaga ggcaaagctc ttgacattta
     1261 aaaaattctt actgttgtct agtgaaaaca tatgcatatt gagaactgac agttcatttg
     1321 cttttactgc cgtgcagact aatagcaaag taaacctatg ctgcgtaata gatttctaag
     1381 acatggttat tggttaatga atgcagaagg gaagttcaaa aggaaaacat ttgttcagac
     1441 aggacaggaa gttgctgatg agtgggccta tgtacacaca tgcgtcattt gtcattgtaa
     1501 tgattatgga ataactgtgg taaaactgta ttttgtcaat gaaggagcct ggtctgatga
     1561 ataatttagc aatggacaac taataatttc taaacctctt atctgtattc atgctatggc
     1621 tgaccagaca acagtcaccc taacgagtca cccatactgt aactatacag catatagctc
     1681 tcaaactatc tcacttcaaa caccaaggta ctccctgcat gcaattaact tcctccgaag
     1741 agtgctcgat catacagtat tgtcatcttg ctgtggcagc gggacataaa agggggaagt
     1801 caaatagtga ctgttaacca tctttttaca aaccattaaa cgggaggaca ttactgccgg
     1861 catagtccct taatgaggac tgacatatgc gagggaggca gttagcacct aacattaaga
     1921 cgcggcggcc cactgatgag atggg
//
LOCUS       KCVB01000577             992 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27259pri ultra-conserved element locus
            uce-201, sequence.
ACCESSION   KCVB01000577
VERSION     KCVB01000577.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 992)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27259pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>992
                     /note="ultra-conserved element locus uce-201"
BASE COUNT          238 a          241 c          231 g          282 t
ORIGIN      
        1 cccccccccc cccccctccc accttccgcc catacctggc ctgaatacca ttagacctaa
       61 tagagcacgg tgtgagggac agccaatcat gatggcgtcc cgtcagtgac accgacatgt
      121 atcttggtgc tcattatcat ccttgaacag cgccaattta catctgatgc tgtgactaat
      181 tctgctccag tgttgtcaag aggcatagga agtggcctgg ccgtgatgac agcgcggctg
      241 tcaggagcct gtggatggag gattcctcgg ggcatgattt aatgcccatg tcaagccccg
      301 ctgtcagcct ctctctctct ccccgctccc tgcgctcctt ctctccatcc agccattagg
      361 attcagccag cggagagatg gagcgatgga gctggcgatt ggcctgctta ggattcacac
      421 tctgggcaag aaaataacac gccctgtttc acaagtgatt accctttccc tgtaaactgc
      481 aattaaggct aaaaaagaaa agcggcaaag aggtgatgga gaaacaaaat gcgtatgacc
      541 gcctagtgaa gaggaaaacc ttgagcattt tccctcactg gtttctggct gcttgtttgt
      601 ctaatttccg ttgacaactt ttccgtggtg ttttgctgag ttgttttggt ttctgtttgg
      661 ttgtataaca cccacagatt tttcgtagcc ctcttgtaga gggattaaca tattgcatga
      721 tgtctctgga tagttccccc atgcactatg caatgcgttc ttgctctcaa tatattaatt
      781 ttggccaggg tagaacagaa ttttagcagt tatctaaatt ttgtgcagca cagaccaact
      841 gttttgtgaa agtttattca gtgtgaattt tcagaattca agacatgttg tggagttgag
      901 gataaaacat gggcctgagt tttttgagaa tgagacagac gctgtctgct tatctgaaac
      961 aatagccaaa cctgagacgc ctgacctgtg tt
//
LOCUS       KCVB01000578            1512 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27299pri ultra-conserved element locus
            uce-1132, sequence.
ACCESSION   KCVB01000578
VERSION     KCVB01000578.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27299pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1512
                     /note="ultra-conserved element locus uce-1132"
BASE COUNT          523 a          253 c          388 g          348 t
ORIGIN      
        1 tgattatatt acaaaatcag tagcagacaa ctggacagac atgagactgt tagaaatctt
       61 ctcatacaac tccgggcaag agtcaaactg tttctttatt attagtttat gtccaaattt
      121 ctaccacaaa tatcaaaata agagctctgt gggtattgca taggatccta tgtttgccag
      181 ggctcaggaa acataggctg agatggtgag ccatcatagt acctgagatg gagtgttcta
      241 tccgcactgt gcgtttgtaa ttggttgaaa cggatgagtt tgtgtcaata aagggattgt
      301 aacgatctgg agaatcaaaa atagtgttgg taaaagaaga acaggtttca acttaaagct
      361 ctacggcatg tttgtgatac aaagcaacag caaactgtgc atttaaagag aggaggacag
      421 gtaactgtaa agcagaatgg ggtgtattgc ctctatagtg ggacatgggg ggtcctggtt
      481 gatacattag aggaattaag gtgttctcac tacaaaataa cacaatctga gctcagtatg
      541 aactgcaatg aggtgcaatg cacaaacaga gaagagatca tttagacact tgcacaaatc
      601 aacattcaaa ctacagcagc acagtaaaca tgcaagctag tcatgcttat aagtgactac
      661 agagaaatga acaagagcga cgagactttt gaggagaaac aaaatgagtc gttaccacca
      721 aacatttcat gaccagatgt cctagaggaa aagctaacac cgtctgaaga cacgacaggt
      781 aagtgagtgg catcgacaac gagttttgag aggaaagggt taaggtttgg catggaatca
      841 tctccagctt cagcagaggt gaaaggatct aggcaggcag aggaaagaga gacaaagaga
      901 gagaaagaga aaagagagtg gagaggaaga ggtgggcatc agagtcagac aacaacaaga
      961 gaaggagagc atgcagaaca ggtcaccaga aaaccaagtc tagaaagaca gatgttggct
     1021 ggaggtgttg gggggaggca gctctttacc tgcccacgct gttgccactg gaagccccgt
     1081 tgagcctggc tgcatggagg gctggaaagt tgactgcagg tcgaagagat cactctccat
     1141 cttcactgca ctgcagacaa aaaaacaaag aacaataaaa tatgaattca cccttttaca
     1201 atcaaaaaca agataaaatg ccttctgaat ttaattgcag ttaaaggtca aaaagatcag
     1261 ttattatagg tttaaagcca ctgaggtgga aatgcaagcg gattgacatt aaaagggtta
     1321 aaaaattgaa gtctgggtat aaaattgctg aaaatgatat aaaaaaaaat catttttata
     1381 cactgtgaat ctaatcagta cccattagag cagctgggtg tagagaagag gtcgatggct
     1441 ggtgctgtgc tgatagtgcc cccggtggtg gagatcggcg atgtatcagt ggtggagaag
     1501 gagggcctct tg
//
LOCUS       KCVB01000579            1359 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27337pri ultra-conserved element locus
            uce-1177, sequence.
ACCESSION   KCVB01000579
VERSION     KCVB01000579.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27337pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1359
                     /note="ultra-conserved element locus uce-1177"
BASE COUNT          335 a          290 c          318 g          416 t
ORIGIN      
        1 gcagcagaaa cgtcagtagg gctgatcctt tcacgctgac ctctgccaca gaatctcact
       61 ctttcccaac caaattgaaa attattaggt aattgtcttg attagttatg agaaatggct
      121 gccgtcaggg gccttgtgct cctcggacat aagacactct ttagtgatag tgggatttcc
      181 cttgtgtgtg cctcagctga tcaccatcat taggtttgtt tgtttgctcg gagattttat
      241 catttgatat acttagattt gtcttttggc tgcactctcg gttttaaaat gtgtgttaac
      301 atgaggtgag tcttgtgttg gaaaacaacg gcggacgtag gagggttgga tggcagctcc
      361 acacctctgc tgacataatc cctcatccat ttccatggct ccataccaaa tccaactgac
      421 ttatttgcct caaatttaga gtagctacat tagaaaaaaa gatctaatca gccctatatg
      481 acatggagta ggccaaggag cccttttgtt tgatttgtac atgggttgga ggggctggct
      541 attggaaatc cctgaagcag gctttagggg caggtctgat tctcgtgtga aatgggaagg
      601 ctctatagct ctcggctgac ttgactgggg ctgtatgggt gtgttggcag ggggagcgcc
      661 ggtcatgaat agggtctttg actacctcag acttgccctg aaaggcagga caaagaccct
      721 agggcaacgg cttagagcct cacagctgag ctacagaatg cttggccgag tcttcctccc
      781 tccttgcctg atttaaatac tacagtggct ttcagcattc tggcggcgga ggctttgcat
      841 tcaccgcaac acatttacca tcaacttttc tctctgtttt tgtttacagt gtactaatgc
      901 cagagttgtt tgcgtaatcc attgcatgga atccatcaga ggtgctggtg aatatacatc
      961 agtgactctc caaaagctgt tttaagatta tcttcatgtt gttcctctta agctgaattt
     1021 atttggatac agactaactg ctggcatatg tgtgcatcac tgccaatcac aactctacac
     1081 ggagtcgtta attatggaac atttttgctt atcttaattc aagatggaga tgctggaaag
     1141 taaacaagac gaaacttggt gcacacctct aatagtaatt atttttgaca gatctgtgtg
     1201 actcttcagc tttgagattt gcaggcgcag aataaaaagc cataaatctt ttctgcagag
     1261 ttaatggggt tatttgagtc ctgtccagac actctctccc cacagagtgt ctagtttcag
     1321 atgtcactca gagctttgtt tgccccccag ggtgaaggg
//
LOCUS       KCVB01000580            1229 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27370pri ultra-conserved element locus
            uce-668, sequence.
ACCESSION   KCVB01000580
VERSION     KCVB01000580.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27370pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1229
                     /note="ultra-conserved element locus uce-668"
BASE COUNT          343 a          263 c          251 g          372 t
ORIGIN      
        1 cttttcacta ataaccaaat atctgaagag ttacttcaca gaatgataaa taatcgtgac
       61 aatttaccaa agccacacat taattgtttt tgtgttaatt acaacagcaa aaaaataaga
      121 tgtgtcttta actgttgcag atagtattga gatactcaaa tgaacacctg ttttgtgata
      181 acatttctat ttttgctgct gtgtgtgcag gttatgagct gtctaagctg gagagtgctg
      241 tcgggtctcc agagaagcct ctgtctgatc tggggaagct gagctacagg agttattggt
      301 cgtgggtcct gctggagatc ctgagggact tcaggggaac gctgtccatc aaagacctca
      361 ggtagacaca caaacacacc gtccgataac aagttacatc agagttatta acatcggaca
      421 cccggccatc ttcttatctg tttaatctca gtcatccagg tagttgggac tgttgtcagt
      481 ttcagctctg attatgatgc tcacatcaga ccatcctccc tctcttttcc tccagccaga
      541 tgaccagcat cacgcagagt gacatcatca gcacgctgca gtcgctcaac atggtgaagt
      601 actggaaagg tcagcatgtt atctgtgtga cgcccaaact ggtggaggag cacctgaaga
      661 gcgcccagta caagaaacca ccaatcacag gtaagtccag atgttttata ttttgcataa
      721 aacttccgtt gattaaactc ctttgttctt ttttcctaaa tgattgtaac ttttacaaaa
      781 aaaacacatt ttaactattg aggaggtgaa accagaaaag attgactgtt ttgcagctgg
      841 ttcattaaac ttctgttctt ttaacaaagt ggtttatatc gctactgtca gaaatcataa
      901 tacttgggac tcaaaacctc cactaggacg ctgacgtgaa caatgtttcc atcagttact
      961 cgtaaatcca gtctgaaaaa acgacatgaa cacattgaag aacacagaca tgatgcagtg
     1021 aactcagggc tcacgagtct gtttctgtct tgttatagtc aaaatgttcc tgcagcgctc
     1081 actgttttgt cgtacagaag tggaactact ttgtgctgtt ggttcttcag tttgtgtttt
     1141 ttttgtgtca tctcatgcag ctcgtctcct agcaacaagg caacctgctg caatcctcca
     1201 aagtcatctg tattctgtgt gtgtgtgtt
//
LOCUS       KCVB01000581            1489 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27413pri ultra-conserved element locus
            uce-943, sequence.
ACCESSION   KCVB01000581
VERSION     KCVB01000581.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27413pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1489
                     /note="ultra-conserved element locus uce-943"
BASE COUNT          292 a          368 c          310 g          519 t
ORIGIN      
        1 ctatctcctt atgagtttga gcagtatgtg agaatgtgtg tgagtttgtg tttgtgtgtg
       61 tgtgcctgtg tgtgtgtgtg tgtgcccgtg tgcagagata aatgtttggt tttattgttt
      121 tgtgttgtat gtgtattatg tatgtatgtt aacgtctgta cgatgtggtt tgtggtgtcc
      181 tcactggtcc ctctcgctgt cctctttcgt gtgtgtgcgc cctttccccc caccttacag
      241 gagatttgga tggccattgg tgggaccatg cgtcttggca tagcagcgag gcatccccaa
      301 tgtctttggt gagacatgtg gagccctgcc ttgacccccg ccccgcccct aaacccccat
      361 ctctctctct ctccctctct ctgtctctct tctcccccca tccccccttc tcccagagtc
      421 ttttgtcatt tcatgtttcc ttcgttttat ttacaccttt gcatgttgtt tttctgtttt
      481 tctttctgtt tttatcccac acctgtaggg gacagtcaaa ggggaaaaag aaaaactatt
      541 gagcagacat ttatgtccga gccgacacac accttatctg agaggcaaaa gaaaatggtg
      601 cgctttgggg gacactcatt tgaagaggac ttggcatggt gtgaaccgca ggttaaagac
      661 tcgggagttg atacgtgcag tagcactacc ctaaacgagg agcatagcca tagtgagaag
      721 gtactgagac aatctagcct gcattttgtt attattattt atgacctacc catccagtct
      781 ttgtttgctc tctgtcacta agtgtaaata ggtgagatgt ttgtgtttgg attttatttg
      841 acgttgtata aattgatttc tcttgcgatt tcgttccttt ttgtttttcg gttacttttg
      901 tctccacctt gctcggtgtt gccgaaaaaa gtcttttggg ggcaccctct ttgcttgcta
      961 cttgtacttg ctgttttgct ccccaaacag aagctacaca tcttccagta gttgtgaaag
     1021 aagagcattg acacacaacc tccctgtttg tcatggcaca caggaatgtg ggtctctctt
     1081 agttgcatgc tcgttctcct ccgtatgttg tgttcttgct ctgctgcggt cctgtttagt
     1141 gcagttggaa cacctttact caggcccgtc cccatcactc tgctgcccta ccccacagca
     1201 cccagtccag acagaacctg ccccctgccc aactttccac tcacttttca agcaaaaccc
     1261 aaatgattgt aagcacacaa aacagggaaa actggatttg ttttgcacaa aaaaattaaa
     1321 acgtttgttt ttcttttttc ccttccactg cgtgtctgtg atttcaccct atctgtgtct
     1381 catggcttta ttctgtgctg tctatttttc atcctttttg atctttaaac aggcacctca
     1441 tggtccacct catttgttct tgtaacttga cctttttccc ccctttcac
//
LOCUS       KCVB01000582            1421 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27454pri ultra-conserved element locus
            uce-730, sequence.
ACCESSION   KCVB01000582
VERSION     KCVB01000582.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27454pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1421
                     /note="ultra-conserved element locus uce-730"
BASE COUNT          522 a          266 c          263 g          370 t
ORIGIN      
        1 acacacacac acacacacac acacacacac acacacacac acacacacac acacacacac
       61 acacacacac acacacacag aggcaggcag aggattattg aggtagtatc aaacaggaat
      121 aataaagcag gttcttaaga ttttaataag aataaaatga aacagtaacc accgaggggc
      181 cagcagggtt aatcccttct atattactga aaataaaaaa aggataaaaa cagacaggag
      241 ggcagagctg gagccggcgg agagagagac agggagagaa ggagaagaag gaggagaagg
      301 aagggggctg tggagatgaa atataagaat agtggtcggc tggtatcaaa agtagagcca
      361 gttagaagtg attgaaatta acaagcaggt tttaaaatag cacaagcagg cttataattt
      421 cctgctcttc cttctgtttt attctcaaac acacacacag acaccctgac acacacagac
      481 aaagatgaca ctgtttgact gtgtttagac ttgatgaaag ctcggttcag tcccttcgcg
      541 ttgaaacacc cgtgtcggtt cagcagcata tttccaatgt caaagcacgt gcgccggcag
      601 cagatggcat gaaacagttg gcaagagtga gcgagcgtga atatctgttt actaataacc
      661 ctttaattac ttcagatggg caatgggatc aaacatccag acccacacag gaattagcct
      721 taaatgagac tggactgaaa atctaggcta accccttatg ttgccataaa gacctttgcc
      781 gtgagaactg ctgcttggaa aaacctttat aaacagaaag tgaagacact ttgaatattc
      841 ttttttccac taagacggcc ttatccataa tcaaacaaca tagcaggata cttttatata
      901 aatctaaaag ggggaaagag aagagacatt catatatggc tgaatttaag gattgttctc
      961 atcttttaaa attctatcaa ttaataattt aatctgtaaa atgtcagaaa atattggtaa
     1021 tatctatcaa agttttatta acccaagatg acatttttaa atctttgttc tttccaaaac
     1081 acaactaaca attattttaa ttatacagtt gctgaataat gcttatcaca ctttcctaaa
     1141 ccaaggtaac atcttaatgt tgcttatttt ctcctacaaa cagtcctgaa acaaagatgt
     1201 ttgggttact atcacaagag acaaagtaag gcagcaaatt ctcataactg agaaactgga
     1261 gcaagtgaat ttgtgatatt ttaggttaaa aaattactca ggtaattatt tggattgaaa
     1321 ggacaccttt ctctttcgac taaaatccat tagatactgg atttttaatt aagacaaaaa
     1381 acgaaacaga cagttcaatt tttttttaca tattttaaat a
//
LOCUS       KCVB01000583            1523 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27500pri ultra-conserved element locus
            uce-775, sequence.
ACCESSION   KCVB01000583
VERSION     KCVB01000583.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27500pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1523
                     /note="ultra-conserved element locus uce-775"
BASE COUNT          436 a          335 c          274 g          478 t
ORIGIN      
        1 atgaatcccc gtaacagccc taatcataca cacacttcat cactgaggct aaaccactgc
       61 acgttaatta tgtagacatc actcaaaata cactgctaca ctgggctctg cacctccaag
      121 agctcatttg tcaactgtgt gtgtctctgg cacacaaaac aaaaggtcaa ccgggtggac
      181 atgatttcag atagcctgct tgttccctta gcggtccttt agacaaattg ttttgtctcc
      241 tgcctttgga ggaaactgtg ttttacatag gtaacaaact cacgggcatt ttgctttgaa
      301 tatttactcc aaactatttt atgttaataa ttttgttttc aaatttcttg catctataaa
      361 aatcttttat tgctggtgat aaaagcattc ctcatttaca ttccctcaaa tttttcctta
      421 caacagtgat tatttttctc agggaatttt cagttttttt cacacaagac ttctaaacaa
      481 ggccaaacaa gtgttaaccc taaccaacaa catatattat ttccttgtcc ttccttcaag
      541 tcctgaccca gactttgaac cccctcaccc cccactctat gggcctggca catgtatgcc
      601 tgctcaacgc cttgttttcc tcatgtcagt ttaattgttc actgtcaggg gcagagcagc
      661 ggataacaag cttagcaaat gatgtctgct gttgctacac attgattaat attttacatg
      721 ttattatcct ctgtccatta ccacagcaaa ttaaactata aatcaccccc tctgctggtg
      781 tcaccggatc ataaattgtc tctcccctag ccgcacgctg cgccgtgcca tgccgtgcaa
      841 ggtcttcgct ttggtgagac aacaaagtgg ggaaagtgcc tgtgttcttt agcctgagtt
      901 atggactagt cacggtcacc atatagatca gagccatgaa aaatggggag tttgttctgc
      961 tataaaacaa aaagaaaagc aagcagccta gtgttaaaag tttgtacctg caagagtcat
     1021 aagcagttat aataaaatgg aaaacatgcg tagatggagg tctaaagctc ataaactcaa
     1081 tgaactattc ttgttatata tatttttgaa ataaacaaat taatgcagta atttaaattc
     1141 caattcatac aaaaatattt ggtaaaatta tagtcaaatt tattcccagt atgttacagc
     1201 ttaatttgca tttggtttgt agcagcactc tgctaaagcc gctacagata aacagcattt
     1261 tgcttcagac agaggggctg ctgctgctgt taaatggaaa ttcagatgga gctgtagata
     1321 aatatttttt ccttgactgt ttagacccat ttttccctgg atatcatcta agatatcaga
     1381 tgacctttcc tgccttgtca ttttttttct cattttcccc acgccggtgg gtgagatgca
     1441 aaggtgcttt accagcagct ggaaatcaat ttatacagtc ccgcgttgca tgtaggcaga
     1501 gatgggacag agcgggcctg ata
//
LOCUS       KCVB01000584            1431 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27540pri ultra-conserved element locus
            uce-489, sequence.
ACCESSION   KCVB01000584
VERSION     KCVB01000584.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27540pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1431
                     /note="ultra-conserved element locus uce-489"
BASE COUNT          391 a          330 c          323 g          387 t
ORIGIN      
        1 cgtgattaga caagtggtaa aaattaatta aacatgatgg aagtagcgaa ggagtgagcc
       61 atctcgcttc cctctcatct gtattgagag ttgcacatgt gcaataccaa aaaaggtgtc
      121 aggtaaagac gtttataggc tcaaacacaa aaaaacattt taattatcct ggacacaggg
      181 gtttggttgg gtggcatgag aggatgtggg cgaaactcca gtttgtcaca caagtttgtg
      241 accgaacgga taaaacagga tggtttccaa aggcttgtct agttcccgat aaaacattgt
      301 cataataaga gccgacagca tctcacttgc atggcagcta agagggctgc tgacaactgc
      361 tggtacttta ataaaatttg tattggggca cataaaaatt gattttggca agtacattct
      421 gacccactaa aaaacattaa cgttgaaccc tgaataccct cagagaaaga gacatccaca
      481 gatgtgccaa tacaaccaca tacatggaca tgcgcaggca cacaagctgc gtgctagtgt
      541 ctcctctcac ctctctgtcg tgtctcagcg gcaacaccag gcaaccagcc gcactcttta
      601 acttccttct tgccttccca aggcccaagg ggttttgcag gaatggattt gtctattagc
      661 tcgtaaagaa cattgctcag tgccatatcg atcatctgag ctgtaacgtt tagggtcttt
      721 aagttgtgga gttgatctag tggtacacgg actcccctgg ctattttacc agagtgagag
      781 atgatcaact taaccctggt gctgcgcttg gccagaggaa acgctataaa cacgtccgct
      841 ggacccagaa actgttttat tgaacctcct gttcagccat cccgaagagg ctgaagggct
      901 accatgaatt cgccaggtgc cttattgtag caatagtgca gtcgtgtggt tttcctctct
      961 ttaatagcat ctcatcacat agttagccat gcaggcctct gtttttgaca agctgtcaga
     1021 atgttggacc ccaccctgtt gtcctaatga tggaccttta cagctcactg attcatagga
     1081 agacctactg gactgtacta ctggttgatg gggctttagg gatgtgtgtg tgtgtgactg
     1141 tgagtgtgtg tgtgaaagag agagacagag taacagagat cattttctgt tccgtctctc
     1201 tctgtgaatg cccctcctcc attctcatct agctgccatg gcaacagtca tcaaatcaga
     1261 acatcagcca ttgctttcat ctcatgctgt ccactggctg cccacagact gtctgtatac
     1321 ataaactgtg tgatggaaac atcacaaagt ccatcctcta cttgccattt taataagcgg
     1381 tcacatccag cacgatttca cgatcaccta cacagcaaag gttgtgagag g
//
LOCUS       KCVB01000585            1594 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27578pri ultra-conserved element locus
            uce-19, sequence.
ACCESSION   KCVB01000585
VERSION     KCVB01000585.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27578pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1594
                     /note="ultra-conserved element locus uce-19"
BASE COUNT          436 a          309 c          321 g          528 t
ORIGIN      
        1 ttttgttttt cacttgcagg ggagataact acaggaagag attattcata gagaatttgt
       61 cttcaaacca ttctggttat cgctgtcgtc tatcttgtat tcacatgagg ttcctatctc
      121 agatacatcc ttcggtaaat gccttgaatc acatgcctcc tttaacttgg caaccccttt
      181 gggatgaaca ggcaagtgca ttaaacagag atgccgcacc attctcagaa aaatgcaaat
      241 tgggaagttg ttagttattt gatgttgctg cacgatcccc cccgccccca aaacatccct
      301 ccactccagt gttggtggtt gaatcgtatg cagtcctgag gacaggcagg gctgtttata
      361 tgcaggcctg gagccccaaa gacagcacag agacagatgc atgtcagaga tagcagatcc
      421 catctcaggc agatgcctct tatcataggc ctgtttgcat aattaggcct tttgatattc
      481 accccctctg actgacaaaa agtataattg cagtatcaac tacgacaaaa ggtaggcaca
      541 aaataaacaa gtcaacataa atagggagaa aagatgcggt gttatttttg tcctgtggga
      601 aaaaaagagg gaggaaaaaa cagcatttta aactaaatct gctgtcagtc acagtcaact
      661 ggtgtgactc ggtgctcaga ggctggtcct gtgaatgcct gcaaaacaca gagcgtgagg
      721 cctgattgta attatgctgg aactaatatg cttttcaaaa tgagccatta tgagctaatg
      781 ctgcactgcc aaagccctgt ctgtttttgt ccctctgtct tggatagttt gcaaaggtat
      841 ggctgatatt gaggccatgg gttatgatat cagagacagt ctgactggga tgagcactac
      901 ttaactttga gctaatactg ctcacaggtg agatgaggga ttaaaacacc tggcacctgc
      961 accactgcaa agggtttttt taatggttct aacagagaat ttgtcagttt ttggttgtta
     1021 ttgcgtttaa ttttgactaa aggaaagtaa ttttttttta tttcatactt tgctttttgc
     1081 acaaaaaatg tatcagtatt gctgctgtat ttttactttt tgttattttg tttgttttca
     1141 tggtgaattt tgcatttttt tcctgttttt ggttccattc tgagtcaacg tagagacatt
     1201 tacccatttt ttagttttgg tttttagttt atccttttgc agaataaagc ttattcactt
     1261 ctttaacttt ctctttgtgt aactcacatg agaaagtagt gttaaaaaca gcttaacaca
     1321 atttgtatgc acctttttga gaatttaaca gatttttgct gaaacttttg actgcgacgc
     1381 ttcatgttga acactcaatc aacgccatag tgaatggttt ttcattcctg attgcctcaa
     1441 tgagttaatg cacgtattta accaacattt gtcaacaacc tggttgacct gcagtttatt
     1501 gctgttgcta tgtatcataa atctgtaacc ttacagatca ctcagttaat tgccactatt
     1561 gagtggacag ctgtccgttt aatcagcatt acct
//
LOCUS       KCVB01000586            1629 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27615pri ultra-conserved element locus
            uce-474, sequence.
ACCESSION   KCVB01000586
VERSION     KCVB01000586.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27615pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1629
                     /note="ultra-conserved element locus uce-474"
BASE COUNT          548 a          341 c          326 g          414 t
ORIGIN      
        1 atgtgtggtc taaaggaagg accagtcctc actgtgctgt atgaattttt gacgcctctg
       61 ctgcttaagc tccagactat caattacgag ataaacaggg tctaggtgat tgttgtcatg
      121 gtgcacaggg ctgctatcca attagatctt gcgccgacta atgtgttaat atcttacata
      181 attggtgttg ggggctattt ttttctccct ctctcaaatg ccatgctgat ccttatagag
      241 actaatatta caccattagt gcaatcttat ctgggcaaag taatatagat gaaatatata
      301 gaaaagtact gtataactca aaaacccatt ccaggggctt gacagactat ctcctgcatg
      361 tttcctagtc atcaccgttt tttagctgcc actttctttc ctgttgttga gtcaaaaact
      421 taatcaataa aagagcataa tgttcctgct gcagaccact tgattgtttt tgggaaatac
      481 atttttatca gttttttcac ttagctgtga aaaggctcac agataaaaca acactagctt
      541 tgttgtcagt tttcgggggg ggaggctggg agataaaaac cacagagact cgcaaaaaaa
      601 aagatttggg acagaaataa gcaatgagag agggaaaagt aaagaaacaa cagagcagag
      661 aatatttgta aagagcaaga gaagagtcag aaaagaagaa ggataaggaa gaaaaaagag
      721 aggagaaaga gagaggggag caagggatga gacgactgcc tggaaaggcg attagtattc
      781 atgcggcgtg gcgctccaga gaggattaat ggactctgat gcctaatatc ctggagaaca
      841 attaatttgc ccacagtttt gtcatggttg aaagtcaaat gctggaacag atcgatgcgc
      901 acagccgcca gtgaaatcgc ggcagcccct tctgccgggc gcagaatttt aatttcatca
      961 tcatcatttt ccctgcctgc caccctcgac gaagctgtcc ctgacatggc tgagagggcc
     1021 gccacatcca aacagacatg gtgcacacac acacacacac ataaagagca cagtgaatac
     1081 acaaacacgc agcacacact cacatacaca cacattcagt gtataagaac tcttatgtat
     1141 gcatacacac acactccttt gatacaaaca caggcagaga cagacaaaaa tcagcactca
     1201 cacacacata tgaatccata cacttctgca tcaatcagta atgcaggatg tggatagaaa
     1261 tgcagacatt tagactctct cctctgtccg aactacatgg ctaaccgtac acacctttac
     1321 ttttatcatc cacaatactt attgacagct gagactttca ccatagagcg acgcacacca
     1381 atgacttatt gttttccacg gttacttatt ataaatggca taaatttgag aacagacata
     1441 aataaaagta aaaaaatgac aaagttttga aagaaaagtt tataggccaa aaatcttcat
     1501 acataccagt catgaagcaa gactcaaggg tcgtggttta aagtgcatac caattaactt
     1561 ttatccatat tgatcctgcc acgaacctaa gttatgtgac accctttaat tctacaagga
     1621 aaaaaaaac
//
LOCUS       KCVB01000587            1770 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27655pri ultra-conserved element locus
            uce-1097, sequence.
ACCESSION   KCVB01000587
VERSION     KCVB01000587.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27655pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1770
                     /note="ultra-conserved element locus uce-1097"
BASE COUNT          489 a          369 c          343 g          569 t
ORIGIN      
        1 aaataagcta agaatacatt gcacaacact gcattctccc atttctaaat ccaatctaga
       61 aaaaatccac acacatgaaa agaaatactc ctctgcctcc acaaagtgaa ggttaatttt
      121 ccaaagcctt agtttctctt tttcagagac ggagcaggta ttctgttgca gttctccgcg
      181 tgccatctgc atcagcacca aagccctcca ctgatgtgga ttagcggttc gctgcacttt
      241 gactcccagt gccacaggat tcaaaacaca gctgttttta tagttggtca gcacaacctg
      301 cacagggtaa cacacacagc ccacctcaaa gacactgttt cttttgcact gtcgtctctt
      361 tctctcatgg tatttttgct tttgtatgtt aatccttaat cctcccatct ttttcctctt
      421 ttctttgtat ttgttcaagc atccttctgt cttccctccc tgtgaacctc tccatcccct
      481 cgttattgcc aactgtccaa atgttttgca cccaatgtca cctgctttgt aatctgatta
      541 cctcacggcg gaattagtat gtatatttaa tgcaggtttt ttcttttctc ctctctgtct
      601 tttattcttt ttaaatcttg atgagtgatg cggggaggaa cggagatggg gtggggttgc
      661 tcttcttgca tgtctggcgg atatgccagg gccatgctaa taaatgacag ctgatttatg
      721 cgtctgtatt cttttattta taaaaaatag caagcgggct gatttctgat attaataata
      781 caagtggatc cactttgatt aggcttctgt ggagagctgt gcatattcag cattacaatt
      841 aaagcctctg atgtgaaata ggaatgacac gctaatgaag cgagctagcg agcctgaacc
      901 gcgactgtcc aacagcatta cggcccatct cggatgaaga gtaattatac gaggaacact
      961 gaaatgtcat taaaaatctt cagtaagtga tttaacccac cattcctcag taatgctaga
     1021 tatttctttt ttttctttac cacctcccaa cccccacatg atcaactccc aaggtttttt
     1081 ttttcttttt ttaaaaaaaa tgtctttctt ttccatgagg ccaagcctaa acttctcctt
     1141 cagaagttcc ataaacaaaa aagggcacca gaccccttaa acacaaacat attgagattt
     1201 gagacaaaga catacaattg catggcccta atttaattgc agaactcagt ggtgccggtg
     1261 gtggtaggta attgaaatca ttccattata aattccatta tgaattggtg attaaggcca
     1321 gaggtgatta ccatggatta aagatgatag ttgggatttt ttttttccaa cataacctct
     1381 tcaaccacct gcacgctatc cacctcaaac agcttgattt atcacaacta acatgtaagg
     1441 gaagaaagta atatgagagg gagatataat gtgtgaaaaa gaggggaaca cgggcgataa
     1501 aggcaaagag tgtgtttgag gggagtgttg tttctaagag gatttagtta atctgattca
     1561 accccttcat tcttgtttga tgtgctcatt ttcacaggca aaagcagcct agaagggttg
     1621 ttattgttgt tgtcaaagca taaagaaaac aaataatgag ctatatcatg ttgccatatc
     1681 atattcatta ggctattgtc taagtctctc tgtgcttgtg tgggtaagtg tgtgttagtg
     1741 tgtgtgtgtg tgtgttagtg tgtgtgagac
//
LOCUS       KCVB01000588            1764 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27700pri ultra-conserved element locus
            uce-788, sequence.
ACCESSION   KCVB01000588
VERSION     KCVB01000588.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27700pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1764
                     /note="ultra-conserved element locus uce-788"
BASE COUNT          409 a          467 c          322 g          566 t
ORIGIN      
        1 aagcttaaca gaaagtacaa ataaataagg gccatcaaag aaattttaaa ataaaacaac
       61 cttaaaattt taaagtacag aaccataatt ctttgcttta atagccattt tccgaaccca
      121 tcaggttaac tcacctgaca tatttttcta tttcttctac agaagttcca gttctcgtgg
      181 gagttttagg ctgttttaat ttgacttttt ggtccaattt cccccctaca agttctattg
      241 aatttaagtc tggttgacat ccgtaacatg ttatttcaaa atttgattca ctggttcttg
      301 agactgacct ccactcatta aatgtgcagt ttcagtgttt cctgtctcag gttctttaag
      361 ccctcttctc ctcctcttac caatgttttc tttacagcat tttctcccag ttaggccaag
      421 ctgacgctgg aacatctgaa cttgtactaa catttaattg accttgtatt tcaggggcac
      481 tttcacagac ttgaatgaaa tgttctgatg tccctgtctg accttgttta gtgtttgata
      541 agttcaattt tcttcccgtt tttttgaggc tcttggctgc agcagttgca gacacttcca
      601 aagctctggc cattttcaat ggcaaacgtg tgtgtgcgac caatttctat acttatcttt
      661 aacctacagc tgttaaccat attctgatgg cagatgctac aggagaacaa ccaataaaat
      721 cacctctatg ctgagacaaa caacactgtc gttttttata tttcagaccg ttatctgtgg
      781 cctacacagt tgtagaagtt tgttgcacat tggtaaaata attttaaaaa tttattccag
      841 gtgttgttga tgaattattc ctaaagcagt ggagtaaata ttatattaga ggatacaaac
      901 actgaaagtg tatgagtcca aataaccaac caccacagca ctccccatct gtacctcatt
      961 cccatcctct tcttgcaaac ctctccaggt ttgacctgat agctaatggt ggtgcatcac
     1021 tgactctgcg gttcgagcgc gcccccttcc tgagccagga gcgcaccgtg tggctgccct
     1081 ggagccagtt ttatgctatg gacactctgg tgcttaagac agaagagaac acgatcccgg
     1141 gctgtgacct gagcggcttc gtcaggcctg accctcttgt gattgcatcc cctctctcct
     1201 ccttcttcag ctccaagcca ggggagaaac ccatcatacc ggagacacag gtgcagtacc
     1261 actgacctct ggcagccggc taatctccct tctggttccc tcgctctctt cccactctgc
     1321 tgtatgcccg tctcttaaag ctctctttct ccttccgctt ccttagcttt tttcccccat
     1381 tcgttccctc cttctctatt tctttatttc ttcctttgtt tttttccctt ttttgtcctt
     1441 cagccgtcag tggaagcgtg acccccatta ctccctctct ctctaatcct ccctaatccc
     1501 tctctcctgt catctcctct ctttgtcttc caggtgctgc atgagcagat cgaggttcct
     1561 ggcacaagtc taaagctgtg ctacctaagc tccaggacac agggttaccg ctccttgctc
     1621 aaggtgacca tgactccagc cgtggtgtcc atgggcctgc tgaaggttca cctgatggta
     1681 gcagtggagg gccacctctt ccagaagtgg ttccatgctt cacccaactt ggcatacacc
     1741 tacatctggg ataagactga tgcg
//
LOCUS       KCVB01000589            1286 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27777pri ultra-conserved element locus
            uce-596, sequence.
ACCESSION   KCVB01000589
VERSION     KCVB01000589.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27777pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1286
                     /note="ultra-conserved element locus uce-596"
BASE COUNT          417 a          247 c          247 g          375 t
ORIGIN      
        1 tctactaatt tcaagctttt tgtgtgtgtt ccttttcata tccttttcat atcataacaa
       61 ctttcatctg agcaagctag taacatttag ggtgttatca aattctctag taaagaaatg
      121 aaggcacctg attggccctc tataaagatg ccagttcaaa taaagaaagt gcaacaaagt
      181 cagatgactt tatgctacct tggccttatg taaacaccat tagaaagttt aatccaataa
      241 agtccaattt actaactcat ttattaccaa taaaggagcg gagaaacacc tgcttcatgc
      301 caccttaacc tcattttaag atctgttttc ttttctctct ctcgttctgt ctggagtgag
      361 tgttatgtgc aatgtggcat gaatttgggt gtctgagaca gcagcctcct cttcctctat
      421 ctaaaccact ggtgggagag gagaagaaag ggagtagggg ggtggattga cccaggaatc
      481 tggagttttt tggaatggga gttcaaagga actccctgca gcctatataa accaccctca
      541 attggcacaa agaggaaact caatttgcag ggagctgtgc tgtatacttt ttatagagga
      601 acttttagtc attaggccat atactccatc tcgctcaaag agccaggcga cgcggcagga
      661 ggccgcaacg tgcttcttct acaaaaaagc gggacagaac cccccaactc cacagaagaa
      721 aaaattctct taatgagagc ccagttagtt cctgtgtctt cagatggtgt cctgtttgtt
      781 cagcaaagca gggggatctg tgttaaccct tccacaacga aatctgctta gaaggtgtaa
      841 tcctatctag tatgcatgta gcagtcttca ggcggggctg tgcaggaaaa acagccaaag
      901 atgctgtatc aaattaaaca tatgtgtgaa aaaaagcaca ctcttttggg caacaatcag
      961 tccccattga aagagaatga atttcttgtg aaaatctagt ttaaggttgt catattcatt
     1021 tcagtcaaat gggatttgag aacaaaacac agtttgaaac atgacaaaaa cagacccaat
     1081 agttgatctg tttataaaaa acattattca ctatgcatac tgtgggtctt tcatcgaaac
     1141 acgtccatag ctgcataaat gaataaggga aagataataa aacatttcat tttaaataca
     1201 gatttaatac acaacacaat cctttgttaa atattgattt aaatattgat ttatatgcta
     1261 taagttataa aacaaaattc aaaaat
//
LOCUS       KCVB01000590            1531 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27818pri ultra-conserved element locus
            uce-147, sequence.
ACCESSION   KCVB01000590
VERSION     KCVB01000590.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27818pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1531
                     /note="ultra-conserved element locus uce-147"
BASE COUNT          490 a          309 c          337 g          395 t
ORIGIN      
        1 attttagtca tcttaggagt caaaatgagc tttattgcct ctgggtaggt gttgagacaa
       61 tcaaaccatg cagggattaa aaaagattaa tacacagtgc aggaaattaa acttcagcga
      121 ctttaacgat attcatgagt ggagctcact tattcacttc aggcaggcac tttaataagc
      181 aaagaataca gtacgggacc caaaagctat caatcaacca aactgatacg cagtttgagt
      241 tgcaaattga taaaaatgcc aggcttcttt caaaatatgt tccccacctt ccaactgttg
      301 caggcataaa tgagtaaggc agatcagatt ttactttaat catttaggtt ttttataatt
      361 ttatcagaaa atgaagtaga atagaataaa gtgctttgca acacaagttt tcaaccttca
      421 aagtgctcat atttgtaaga ttatttgtgg actttcgcct ttgttacaca gttgatagtg
      481 gagagcaaca ggaaacatgg tgaaagccgg aggaagatga aatgtgagtc aggtcttgca
      541 gcaggacact tcaaggtcca atgttacaga aaaaaaatga tctaacagca agtcggaaaa
      601 taaagaggca aaacaagagc tacaaactcc aaaaggtaat tcagtgacct agatattaaa
      661 gcttttctgc catttcgctc tcccctgcct tgtccaacca tcatgttgag ttaaggcaag
      721 cattatgctg ggaagactat ttgtttaggt gaggagcagc cgcagagcta atatgagcac
      781 aatttgctca gcaggtcaca ggcgcagagt ggaggaagta aatgaggcag ttctgccccc
      841 tcgggtgcag cccctgcctc atacatcact ggatgtttta ttcaatacag ccaggaaggc
      901 tctatggcag gtgccctgac tgtgggggcg cctgccctgc tgtaggggcc catattacat
      961 tcagcgagct gcctgtctgc agggctgctg gagagaaaat gactggagcc agtgaagaag
     1021 ataaattttg aggatgcttg tagtctaatg ataaaacata ctgggtgcag cttttccact
     1081 gcacagtcag cataatgtgc aggctcattt gtgtacaatt tggttgatca agttaaatat
     1141 tgcaaagcaa cctcagcaac ccacaaggct caaagttcac agaggagtcg aaacagccta
     1201 aagtttaccg gccaacactc acattcaaat gaaacgtggg gctccagtta cgttcaagat
     1261 aatcagactt ttgtgtttgc actcaaggcc cagactgcag aggacaaatt tgctgaaaaa
     1321 ttcacaggac aatgacaaat tgcctgcaga cctcaatgca gcacagagca tcatccatgt
     1381 cgccgagcac aaagtgaaaa gttttacagt gtttcgctca catctccatg aattttatga
     1441 gttccttgat aaaagcctgt cacaatatgg gggcgcacta taaaaagaga ttgtatatca
     1501 ttaacactcc agggtgaaaa atcactaatt g
//
LOCUS       KCVB01000591             414 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27851pri ultra-conserved element locus
            uce-102, sequence.
ACCESSION   KCVB01000591
VERSION     KCVB01000591.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 414)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27851pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>414
                     /note="ultra-conserved element locus uce-102"
BASE COUNT          103 a           85 c           92 g          134 t
ORIGIN      
        1 ccgtggagcc agagggcagc aatgctgtaa tcagtctgtg tattgtcaaa gtgcttacag
       61 tgcaggtagt actatgtaat acctactgca gtggaggcac ttacagcaat accttgacac
      121 cgatgtgcat atccattgat gttacatgtc atttgaagta ctgtctttgt gctaaggtgc
      181 atctagtgca gatagtgaaa tagactagca cctactgccc taagtgctcc ttctggcata
      241 aggggctgtg atatgcgcta ccagaccctt ttttaatttc taattgggct caaatatctc
      301 gcagttctct gctagttttg catagttgca ctacaagaat agatgagttg tgcaaatcta
      361 tgcaaaactg atggtggcct gcttgctctc cacctgtaag attttttttt tttt
//
LOCUS       KCVB01000592            1547 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27882pri ultra-conserved element locus
            uce-1175, sequence.
ACCESSION   KCVB01000592
VERSION     KCVB01000592.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27882pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1547
                     /note="ultra-conserved element locus uce-1175"
BASE COUNT          471 a          285 c          329 g          462 t
ORIGIN      
        1 gttattgtca caaagaggat tcgtagaaat gggaggaaac acatatgttc cacagtcccc
       61 ggtggaagct tcgcactgca atagtttaac tgatttgatt atttatttta ccctatagaa
      121 ttgaaaaatt tgcaatattt caatgctatt ggtggcgttg cctatagtta aaaaatgtga
      181 taaaacaaag tgaggatgac gcccgcaggt aattaattat gtttttaatt atgtgtttat
      241 actcttctcc ctacaggcag aatgattatg gacaagttgg cagtggttct aatgaaaatg
      301 cggtcgtccc atgctttgtg gagtgtgtgg accatgtgtc gaaggtcacc tgtggggcaa
      361 accacaatct tgcactgaca ggtacggtca tgcaccatgc aggcctgcta tgacacacaa
      421 cacacaatcg acgtgttacc attaaataga ttttgattaa aatatttggc ttttaaaaat
      481 ggagccattt ttagttgtcc tcacgttaca gtatactgta tacagtttat aactatatac
      541 agtataccct gtaactagtt acctctcttt ctatagtatg ctccaaaata caagaaatgg
      601 atcctatcat tgacctttca cctattatgc tagattttat attccgtaaa acgcaccagc
      661 acaaatcagt tggacagtat ctttgtgaaa tggagctgac gtcactattt tactcaattt
      721 tatgcgaata tcagagccct caagccctca ttctgcacat cacctgagag agagacagga
      781 gggagagaca gagagttagc gagaatatgg ccctggctcc tgatccataa cagtcattgc
      841 ctgttacata catttcagaa ttcattcagg ctatgaagat aatcgaggta gtcgacagtt
      901 tcccctgcag cccaatggat ttatgatagc actgtcaaga aaatgaggcg aaatgacaca
      961 gtagctgact ttagctgcaa ataatgtgat aggccgtaca gtggtgcagc agcagctgct
     1021 gtcattgcag gtaataggct ataaagaagc ctgatagcaa tacctgtcat ttcttaatgg
     1081 ctgacttcac tgaagagctt ttgtggcctt taacatgaag ataatggaag atacaacaaa
     1141 ttttacagct tttgcgttgc atccataatt tatcaccaga ggctccatgc agcagcagca
     1201 caacaaggac aacatgctcc tagaggcatg ctggagagag atgcaagatg agctgcaggg
     1261 gttagaggcc tgtgtcatag tttgcgatga acagaggaac agaggaactg cacagggtcc
     1321 aaaacatctt ctttcagttg atatgatcat atagctattg tattatttga taatacattt
     1381 gtgtactaca tttgtttgga aatgatattt tggaaatggc attaaaacag tatagctgct
     1441 agatttgaag aaataatctg ttgatataac atgattgttt ttttgctgaa tttgaaacct
     1501 ttattaaact gctcattgat gacatgcagg tgacggcagg ttgtttc
//
LOCUS       KCVB01000593             816 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27922pri ultra-conserved element locus
            uce-246, sequence.
ACCESSION   KCVB01000593
VERSION     KCVB01000593.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 816)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27922pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>816
                     /note="ultra-conserved element locus uce-246"
BASE COUNT          207 a          198 c          173 g          238 t
ORIGIN      
        1 gagcttactg attttttttt ttttctcttg cgagggacac tttaaaaagc tgtcatgcac
       61 accaaccaca ggcatttccc tcctccctcc cctcccctct cccccctctc ttcctagttc
      121 tccttttttg tgagatcctc taattttgcc catgcctagg caaatttgtc gcggcgcgtg
      181 ggcccgtttg atttcattaa acagaatatt taagcataaa aattgtttaa tcgtctcatg
      241 tcattaggag atggatttca ttttgcatac atttgcatac ttccccctca aattaaacca
      301 ggcaaatatg caaatgcaga ggcccaggtt atggcggagg tgtttattgt catcacttca
      361 ggcagttcca cactgcagcg cctccgcaga acaggggtga cagcaacatc tggacgcctg
      421 ggggttggga gggaggaaag ggcggaggca gaaggtgtcg gtgtggggag gaaggtgtgt
      481 aataggctgc ttttaatttt taccattttt aacaccaggg cacactgtgc atgcgctgat
      541 ctaccactgc aaatactgca gcatctagta cacaaaaaac tgaatatccg tcatcacaat
      601 ataatgactg atttaattac caacttcatc cctttggacg tagacgcgct aaaatgtata
      661 gtgagccaat gaagacataa aacacagcac tgttctttca actgtagcct ctgcatgtgt
      721 ttttatcagc tgttctgctg ctattgtcca catggctaag cactgctgct aatgaggcag
      781 taattagcaa cagcgtaacg cttcttcacc cgtgct
//
LOCUS       KCVB01000594            1628 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 27965pri ultra-conserved element locus
            uce-697, sequence.
ACCESSION   KCVB01000594
VERSION     KCVB01000594.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="27965pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1628
                     /note="ultra-conserved element locus uce-697"
BASE COUNT          493 a          335 c          323 g          477 t
ORIGIN      
        1 aaaatcctct cggtaaaact tacaggaggc ttaattcaac aatgcagcac attcagtcgg
       61 ctgttgtgtt aacaaagtta gattgatcta acaagcttaa agatgtcagt gcacatagat
      121 gggatgatgc aatcaaacgt caatgaattt cgacaagagg caggaaatgc agactaatta
      181 ctaatgcaga ctaattactg agtctccact tcacgagctg gtaacagtga aaaagaaaaa
      241 ctagaaaatc gtctcatttc tcatcaaaag ataaataacg gagcggcaga ctgtgtcgca
      301 aagtgaggcg agagcagagt gtgagcaaag gagtcagagg ggacaagaaa gaggatgtcc
      361 ttaaaaagag tgttttcttt taaaattaaa tacatcttca aaaggaaacc ccacaatgtc
      421 tcgctggagc cacctgcaca cagagggcag gctgccactg tttcaatggg cgctgcatgc
      481 acaccccagc aggcttccag atagcggcta aagagcttat ttcccaaatc tctttatcct
      541 ttctagaact gtgagacgtt gacagaaagt cagttaactc tagccagtgg cagctgcatg
      601 ggttttgcat aattaattat ttatacggag ccagacatag taattaccat ccagatgaat
      661 atgatattct aatcaggcag gctttaattt taattgccct ccagcatcta actgtcagtg
      721 ctggcctggc ttgataaagt ggagagtctc taaacaccat tagacatctg ctccgagagc
      781 attgcctcca ctacacacca gccccaccat ccgccataaa aatcaataca ggaccagggt
      841 gtgtgcgtgc gtgtgatctc gctcattttt ctatggaaag ccatttttcg tcaatgacaa
      901 aaaccagagc agggcttcaa gctatggagc cccctccctg atgattgatg aggcctctcg
      961 gatttagtgt ttttcttttt cttttctttc ttttttaaga atttggagtc tcatctcaat
     1021 atattgtatg aaagggctta tcatgctcct tttgtcttct tttaagacca gtaaacagcg
     1081 aagattgagg gggttattta caatatcttg gcttatttag gcaaataatc tcttgtatgt
     1141 aaacagcaaa gatattctaa attttcctac aatgaaacat ctacagttct tctctatatg
     1201 ccggatgcat ctttgtaaga ttggctgatt gtgtgacagt tatatgatga tttacacttg
     1261 tggctcaaca atttctcaga tttggcaatt tcacatctac tgatgcccag ctttctgaga
     1321 ccccttgaca gatctcagta cagcaggcac cataatccca acagctgtta ccatggcaag
     1381 gtttttttct ttctgtacag tttccagcca caattccaat agaaaaatat tatcacacat
     1441 ttcacagagg tcttacagtt agagcctggg ccagctgtag ggattgaatt gcatactcta
     1501 attgtagata cttatggtat gtgatgaaat ataaatttaa tatgattata tgttgctatt
     1561 aaaaggaatc tttcgctgta tatcagcgac aaacagcaca gctcttttaa ccttgaggca
     1621 taaatact
//
LOCUS       KCVB01000595             958 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28004pri ultra-conserved element locus
            uce-1188, sequence.
ACCESSION   KCVB01000595
VERSION     KCVB01000595.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28004pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>958
                     /note="ultra-conserved element locus uce-1188"
BASE COUNT          212 a          193 c          324 g          229 t
ORIGIN      
        1 acgagtaaaa tgtcttcttc atcatcagcc tctaatcatc cagctccact tttctgtcct
       61 ccatgtaacc aaccagtctc catgttcgtc agcgctggta aaagaaaaaa aactgctctt
      121 ttgcccctca aacacggcag ccattttaga tgtgagtctc ttccaaggcc gctggacatg
      181 agccagagag agagtgagcg aaagaaagag gaagataaag agagcttggg tgtccatttt
      241 agaaaaggca gagagaaaga gaagtctcca tcttggttca cctctacaga caggtgtgtg
      301 tgtgtatgtg tgtgtagggg ttgagtgtgc acattaggag gcgttgagca ccgagcgact
      361 atacagagtt tggtagtaag cgcctgagtc catggtggct ggtcctgggc tgtcgtagat
      421 ctgcttggct cccactggag agccgccggc gtagccgttg taggccatca cctggtcctg
      481 gtaggacttg agatccatct tctgctcgtt ggacatgagg ttggtgatgg agaagggatg
      541 gttgaagttg tagtgggggt ccagggactt gagagcatca ctctgtaggt ccatgtgatg
      601 ctgcatggtg ctgggcagca ggtgggtgtt acccactaaa gactgggagt ggaggagcgg
      661 gttagaggca gaggaggaag tggctgagag tgacaaggat gatggaggga tggaggatga
      721 ggtggaggag gaggtaggaa gggagacggg tgggctgtgg aggtgtgagg aggtcagtgg
      781 agggcagtct agtggaacca aggagtttcg gggatgcggc tggtcgtctg aggagcctgg
      841 gtgagagttg tctgagtggg cggagtcagc tccctctgat ccttctgtgg ggctgtgatc
      901 atcccccaga tgatctccac tgtggcctcc tttccctgag ccctcctggc tcttacct
//
LOCUS       KCVB01000596            1379 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28047pri ultra-conserved element locus
            uce-1095, sequence.
ACCESSION   KCVB01000596
VERSION     KCVB01000596.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28047pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1379
                     /note="ultra-conserved element locus uce-1095"
BASE COUNT          388 a          322 c          298 g          371 t
ORIGIN      
        1 taatgagcca cactacacaa ccctcgccct cacagcagtg acaacaatag acaactttct
       61 cagtacatta gcacagtaag gcactgagcc tttgatatgt ttaataagag ggttaatgat
      121 gtaatgccct atggaagtgc caatatggtg agaagctctg acaagaagtg gcactaataa
      181 aggctgttat ttaacttcag ataaatgaag tcatttatca caatgagata cagggacccc
      241 tccttaaagt tcgataaagg tttgaaatta cacaaatgat tttggccctg tgatccatcc
      301 ataaacgcct ctgatgtttt ataacaggtc caatccagat tcgtccggcc tgtctctact
      361 actgtcttaa tgtaatgttc acctgcttgt tatagtttgg aggctgataa gaagtgatgc
      421 ttgtacgtct cccttgggtg tgattgtgag gtaaatatta gcaggccctc agcgccaccc
      481 cataccagta aaccaaccca ctgtcctctc ataaagaaat caacagagtc aatgactttt
      541 taaatgttat tatgctgtca tgaggcttga gatgggtatg tgcgcgtaca aaatgagtgt
      601 gaaaaatgtc ctggagggaa cgtgtgtatt tgcatgcatg tgtacacgct tgcatgcaca
      661 tgctccttct gttcattatt ggccagcagc agaagccagt catttccctg tcttcataaa
      721 gacttggccc attaaattct acctccagtc aataattagg actccacaaa taaaccaaag
      781 gcaattaaat caatgcttcc ccgggccgga gcctggaccc catggcccac atggacactc
      841 catcttctct gctggcctta ttcatggtgg gttgcagtgg agccatacag tacaccccct
      901 ggcctgctca tgattattag atagcaccgt ccctcgatcc tggccataga gcgctgctta
      961 tggtggctct ccactgagag gcatagggtt tgatttcatt aactcaccag tggaggtgag
     1021 agacgaggaa atgaatgcaa tgtttcattt ggttttgagt ggacatgcgg ggcagcggtg
     1081 agggggctcc agaaatgtat gccacttctt aggtatgaag ggagcagtaa ctcaagacct
     1141 ccaccagcac gtacatccat tcctgcaaca caatgtttgc caatgtattt tggacgtctc
     1201 tctttcttgt tatgttgaca tgtggcagca catgacagta atggtaaaca atggcccaca
     1261 cacagacaca gatacacata cataccactc ttatgctctt tcacatcttc acacacacac
     1321 agacacacac acagagtgag tgatgggttc gctattgaca gggccactga gataataaa
//
LOCUS       KCVB01000597            1591 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28088pri ultra-conserved element locus
            uce-433, sequence.
ACCESSION   KCVB01000597
VERSION     KCVB01000597.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28088pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1591
                     /note="ultra-conserved element locus uce-433"
BASE COUNT          480 a          287 c          305 g          519 t
ORIGIN      
        1 ttcatgtctc tgtttagaac cattttccca tatgtttgtc attaatataa aggaacataa
       61 aagtggattt gtatactatg tgccaagaca aacagtggaa tataggctac caattttcct
      121 cttagcctca gcattcagtc atgtgagaag tcctcattgt caatttgctc ttggcccttt
      181 atagcaggtg gtgcagagca tctgcaatgt ttcacaagag gttaaataga attcaaaata
      241 attagttgca ctcagaacat gttacatgac ggacattttc tccatttaag cagaatgcac
      301 aacactgttt ccagcaaaaa ccgcttaagt gttaaaagct gtagaatata ggctaaccta
      361 ttctaatcct acagttttaa agctggaaag taattaaaga caaagggatt gatagtctac
      421 ttttgccctt gactgagaga cagagggagg gagataggga caaagtatat gtgtgtgtgt
      481 gtgtacgtgt gtgggtgtgt gtgtgcaaga cagagaggga gggagagtga cagagagata
      541 atctcttttg tatttttgat ccctaaatgt ggtggttgtg tccccttatc ccagctactt
      601 atttctgcag tgcgtaagtg atgccatgca cttaggctgt gaaaagcagg tcagtatgac
      661 cactgagaga aaaccacata tgcatcacat ggatagctct gggattattc ctcacaaaat
      721 gtcttaattc ctctgttcac taatgatcca gggaaaatgc cctctaatct ccattccatt
      781 aattaaaaag agcatgagcc catggaaaac ctcaccatct gctgcagttt ctttcctgca
      841 gacagattgt gttttcagtt tttaaccagg ggaactgggt ttttcagagc aaacgggtgg
      901 agcgtgcatt aaggtggatg gtaaacagat acaactgctg tatttgacta ttaatacact
      961 gaaatatgct gtaaaagatc tctttcaaac aggaacaatc cctgccagga aacggggatg
     1021 acgtctggga gtttgagcag cttcctgagg acaatttaca atttatagac ttactcatac
     1081 tgtacatagt ttcactataa cattcctctt tagagacttc acagtcattt tatagtaagt
     1141 cactatttct attttatgag aatgctgtaa tacagtccat gtattcttct gaaggattgc
     1201 catgattctt tttttcatta ggtaaggctt taacgctggt ttgactttat gctttctttc
     1261 ctccttttca gtgatttgat aaaggctcag ctgaaattct cacttccact aacagcataa
     1321 acacacacaa aatggatttt ctcattgatt tccttccgca gtttaagttt cttattagaa
     1381 tgagtttatt gaaaagtttc taatagatta atttcatttt aaatctatgg ggaaattaca
     1441 ttcgatctta cattcatttt ccaacatcct gcactttaaa aatataatac tccattaaga
     1501 aagctgtttt aaaagtaaaa ataacttaat taacaatata atgtctacaa ctgtaacttt
     1561 ttaccgggtt gtgagtttga tgcaatatgc t
//
LOCUS       KCVB01000598            1468 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28123pri ultra-conserved element locus
            uce-476, sequence.
ACCESSION   KCVB01000598
VERSION     KCVB01000598.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28123pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1468
                     /note="ultra-conserved element locus uce-476"
BASE COUNT          473 a          294 c          333 g          368 t
ORIGIN      
        1 gagtattttg tgtatgtgtg tgtatgtgtg tgtccgtgca gcatttttta ggatcaaaaa
       61 cgcgggggaa ataaaaaggt attaacaaac ccaaaataaa attaagaaag gagtggcatc
      121 tgaaatgcct acagtagaaa ggagcacaat gattctttgc acgagaggaa gcagtggcat
      181 gcactcgcat acaaagggga acaagtggtc tttacatgga gtgcagctgc ttattaagac
      241 tcactccttc gtgcattatt cctttcatcc ccactcaacc acaaagtgat tttactttgc
      301 cactcacaaa gacagcaaaa gatttgtaat ggacaaatca gtcgcttccc cacgagttat
      361 aatacaataa acagcacatt ctataaaagt atctgactga aaaacactgt gtgggagaga
      421 gcgaggaaga gagagggaaa aaaaagaata tgaaagaccc cgattctctc gaacgccata
      481 atagtgaaat gagcacatat aaatgtgtta agagtatctc tttgcatctt gtatagactg
      541 catgtattta atctgtaaag cttattaagg ccctagtcat tgctatgcaa agcagcaccc
      601 agtgcttttc tctcgctttt gagccgagtg cagtgtttgg ccaataggac tggccccccg
      661 cctctcattc tgcattcata acatgcaaca tttgcacaat gtatctctca aaggccctga
      721 atagaaggcc ttaatactca tgtaaacaac tgcaaaccaa ttacttaatg aatcgggccc
      781 acacataatg aggagcctca caaagggcta taaatgcatt ttttgcacca aagcctctgc
      841 tttaaactac aatccctctg ctgtagtcta ggcttaactg gcaaatcata attattgtct
      901 aagacaacag agaggaagcc atttttgtat tgtgagtcca gctccatcct ccttttgatg
      961 tcactcatca gctcgctgac aaaataaagc aaataattaa caagtaggca acgaggaggg
     1021 aggggggtga caggaagaca gagaggaaga ggggcaaaga gatggagaga gggcaagaga
     1081 gagagggagt gagacacaga gagaaaggag agaagcaggg caagagcatg ctgcagtgtt
     1141 atgcttctgt ttaagtgcag ggcttctaga ccacttgtgg aagcaataaa gcacaagaag
     1201 tgactgtggt aagtggaatg ctgaaatctc agcgtccagg agaagcaaag aaaagacagg
     1261 gggaatcata tgaccaaaga aagcaagata gcgactagta gctcttggcg ccgcttcctt
     1321 cataaccgtg ctgagattag actaccgggc atgcgtaaac ccattcgtcc agtttgactt
     1381 atgtacacaa cttgtgtctc atcactgctc atggcattct gtttctgtct aattttttct
     1441 tctgcaaatt tcgtttaatc acacatat
//
LOCUS       KCVB01000599             948 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28164pri ultra-conserved element locus
            uce-819, sequence.
ACCESSION   KCVB01000599
VERSION     KCVB01000599.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 948)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28164pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>948
                     /note="ultra-conserved element locus uce-819"
BASE COUNT          228 a          253 c          190 g          277 t
ORIGIN      
        1 tggccccggg aaccactgat cctggatcag aggtggaaga gactgtggag gtggagtaag
       61 agggagcaag acccgggagg gttagaagaa gctgccctgg agagatgctc gcaggctggg
      121 ctcagttgac atccatggct gggtgacggt ccaaagctct atgtagagcc cccctctcct
      181 gtttgttgga gagaggtggg actcattctc catcagttac acacacacac tcacacactc
      241 acacatccac aaaaacacac acctttctat ttctcctttc ctgtttttaa ttctctcatc
      301 ctatctccct tctcttctct gtctctctca catattcttg cagactctca ctctcttcta
      361 tttctgtttt ctctttctcg cacacacgtt ttctctcaca cacacactca ctcacacaca
      421 gggatctggg ggatatatag gggtgtggta gagctagtga tggggctctg ctttgatgcc
      481 cagtgggaga ttacagggcc aagaagcaga gataaaagta caaccgaaac acaggtatct
      541 gctcttgctt atgccttaca aacacattca cacgcacact tacacacatc cataacacat
      601 gttccgagtt ggtctagaat gcacacacgc tgacacacac ctacatacac actaacatct
      661 aatggctagt cagtgacact gaggtcttag cccctgtctg ccattcctca tttgtttccc
      721 agcccctcct atgtgtgtgt agcagtagca cttgctaccg ggtgtcttgt tactcctcag
      781 aaggtctatt gatttaactc ttgccacaat ctataacact attgtcttgc cttaagtgtc
      841 tcatctgtac aattcactgg caggagtcta tacatcaaaa accccgcctg cttaaggata
      901 ttggtattct gcaacatgct gggaattctt tttttttttt tttttttt
//
LOCUS       KCVB01000600            1365 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28208pri ultra-conserved element locus
            uce-732, sequence.
ACCESSION   KCVB01000600
VERSION     KCVB01000600.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28208pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1365
                     /note="ultra-conserved element locus uce-732"
BASE COUNT          374 a          305 c          332 g          354 t
ORIGIN      
        1 actagtgcta ttgctgcgag tgggcttatc tgtagaggcc gaggattgca cctgtggtgg
       61 tgtggaattt aagttcagcg atgaccacaa ggggctgctg aggaagctgg agtagatagc
      121 cttcatttgt tctaaggtgt ccatgcctat gggagggggg gttttagtgc caccattcag
      181 agatgctgtg gccaagttgc cctcagcttt tctggagggg ctctcaaagt ctgagagacg
      241 gtcactggtc tcactcacat gtgactcact gtccatctct tgtcccgaga gctctgcggc
      301 cccagctgat tcctggtcac atgcctgctc cttggcaggc cctacgcttt gctccacaaa
      361 ctcatctttc atgggaaggt cattctgggg ggccgagggc aggtcgtctg cttcctcatc
      421 ctccacagtg tgctctgtca gctcctcagg agaataggct gcaaggagaa cggagacaaa
      481 gaaagaaaga aagagatgag ttaaattgca gggagtgaac acgctatgtt agaataactg
      541 gagaaaaagt ttccctgcta tgtgtgcccg aggactccca gggataaatg agccatctgt
      601 gtgggagcct ttaaagctga gctgagaaat tacagtcatc ccatttcacc tcatcaaccg
      661 ttgaggtgtt attttcctct aggcgagcct gtatttatat actaccccag ccacacggaa
      721 cttatgcctc ccctcttttc actccttcta ttggaggaaa acaaaaaaga aatatcttgg
      781 caaaaacata atgcgccatt ttatttatct ctgggcgcaa cagcttgaaa tgaaaactaa
      841 atctgaaatt aatgaagccc aatctcactg tggcattctc ctctggggta ataaatgagt
      901 tggatcaacc tccatctgtc agttaatatg tctgacgcct cttcatctgc ctcttctctg
      961 attacacaca tacacatgcg cacacagtca aagagcatat cttgaagaat ttcaagaaaa
     1021 ggatgtcaga attttgctga agatattttg atatgttgaa tctcccagtg tcatctggac
     1081 tgaagtgagt tcattagttt cttcatgtgt ggaagtcagt cagtactctg ggttcaagtt
     1141 gcattgttaa atgtcatgca aacataaaca aagacatttc actcaaggaa aaggaaaaga
     1201 aataataaaa aatatgggag gaaggagcac tggcgaccta tccagggtgt accctgcctt
     1261 tcacccaatg tcagcaggga tagactacag ccccccgcga ccccgcaaag gataagcggt
     1321 tactgacaat ggatggatgg acggaaggga agaaggtaat gagac
//
LOCUS       KCVB01000601            1285 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28248pri ultra-conserved element locus
            uce-777, sequence.
ACCESSION   KCVB01000601
VERSION     KCVB01000601.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28248pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1285
                     /note="ultra-conserved element locus uce-777"
BASE COUNT          353 a          254 c          249 g          429 t
ORIGIN      
        1 acaacactct ggagtgggaa ctggaagcta attctactaa atgtataata ctattaatat
       61 ttgtaacgcc taatctttat ctgccacttt gcagaaatac agggattaaa cagaataatt
      121 agacatgcaa aaataaaagc tccgcagcat tcaggtgttg attaaaactt caaatgtcaa
      181 cattatgaat gaagcttcga actaatattt actgttataa atataagtcc aactgtgtgt
      241 gtatgtgttc cttcctcctc cccttaccag atatgagctc agggtgatta tttggaatac
      301 agacgaagta atactggagg acgatgatta cttcactggg gaaaagtcca gtgacatatt
      361 tgtcaggggg taggtacaga gggacgcggt gactgagcct cgtcccgtgg gtgtgagccg
      421 tgtgtggatt ttttttaatt tttttttgca gttaaccatt attttttttg ttttgtttcc
      481 atttttttct gtgtgcgttt ttgtgtcgtg tctgtctttg tgtttgtggt gtgtctcctg
      541 tctgtctgtc gtcctctctc tctcctctgg tcaatgctct gctggtgtct agctttgagc
      601 ttcgtgttgt tatttggaac actgatgacg taattctaga ggacgatgct ttcatgacag
      661 gagagaagat gtctgacatc tatgtcaggg ggtaaacaca cgattccatc gcatggctcg
      721 tacaagtacc tcccttcctc catcctgaag ccctgatgtc actcctttct ccccttcccc
      781 acttcctgtc tcaactctcc atccagcacg taacctcgct tgtcctgaac tctccaaata
      841 gcacgctaaa aaaacgattg ctaaatgaaa aatgagttca gccaggatca aaccacatta
      901 accacttgtt gaatacaatt ataattgtac ttattgtata aattacatat aaaaaattca
      961 ctactagaga ttgaaagttt gttcgtaacc ttggagacag cagaaacatc tcagctaaca
     1021 tgaacacaaa gtgctttgag gaattgacaa attatcctga aaaacgggca actccacctg
     1081 ctgagaaagt atttttattg tgttttcgtt ttttgtttta atgctgtctt actgtaaact
     1141 aaactcaaaa ttaaaagatc tgaagtatgg gaagaccaag agctcctgag taaaccttgt
     1201 ggtgagatag ttttgtcaac ttcacatgtc gggcatgttt gtcagactgc tatttcctta
     1261 aatttttttg tcaaaatatt atttc
//
LOCUS       KCVB01000602            1286 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28287pri ultra-conserved element locus
            uce-9, sequence.
ACCESSION   KCVB01000602
VERSION     KCVB01000602.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28287pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1286
                     /note="ultra-conserved element locus uce-9"
BASE COUNT          404 a          238 c          292 g          352 t
ORIGIN      
        1 atattgccac aggttaatca gaatatgttt gcagacacag actgtgaacc agtgggaaaa
       61 aaacatagat tatttctcac tgactcattc aaagctgtaa gtggcactca aataacagac
      121 tgctcacatt tagctacaaa tacatcattc atacgagtaa atgattttgt actgtaacaa
      181 tgatgaatag gcaaataaaa atcatacttt attgtttgca aaccccgccc tcaatcagat
      241 aagtcccatt acttcaaaaa tgattacaaa ttctgaatgt atctgccact attactgtta
      301 acattaaaga gtgcctttaa gatgctcgag aacgaaagct tgacagtggt aacagtagcg
      361 tagttgggtt tagtgaatgg gtagttaaat ggcaacttat agttaggcta agtggcagaa
      421 agtggtatac caaaaaagaa tatataactt ctggtctgct ttaacctcta agtaaatatc
      481 tggagtttac ctctaggtaa atatctgatt atgtgtgaag gaaaaagtca cgaaatggca
      541 cacaggccat cgaaagtgcc agttcaacct ccactccccc ctcctctctc gctggccata
      601 aaacgcagtg taataggatt cctaagcttt tccagtgact gcttccacta tcacacaggc
      661 atttaagtgc actaatccat gacaaattgt ctcatctgat ccatgacaaa tcgtctgagg
      721 ggccgataga gacgaggaga tggaggggtg gtggggattg ggtggtggtg gtgagggggg
      781 acaagaggat ggagggctgg ggggagaggc caggcgtaga gagagtgatg ctcggcacct
      841 gaatgatcag gcagttattg cctagttttt aaaaaggttg ctttcattac ctcggagggc
      901 atgctgcgtg atagaaagtg ggatcacata gcggtgatga gctctctacc taagcccaca
      961 gttttcaaag caattataat cccatgcgga ggtacattaa aggatttcag ctcctctgtt
     1021 ggcaaaaggc aggatttgta gatgccatgt tcaaaaaaat gaatgcttgt aatttaattt
     1081 gtcataaata atttatgatt ttcatcaccc tatagtttaa tttggctctt aaggaggtgg
     1141 ttgtaggcaa cagaatacac acagaagtga atcttgagtc aaacaaaata gcactgatga
     1201 aaacactcct gctgcgacca cataaaaggc tattccacaa cctctgacag taatgacttg
     1261 aatgtgtttc tgtggtaatg tgtgtg
//
LOCUS       KCVB01000603            1857 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28329pri ultra-conserved element locus
            uce-26, sequence.
ACCESSION   KCVB01000603
VERSION     KCVB01000603.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28329pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1857
                     /note="ultra-conserved element locus uce-26"
BASE COUNT          540 a          388 c          410 g          519 t
ORIGIN      
        1 cgttggtcaa gtttattagt tttattgtaa ctagagtcta ttaaaaagca catagactaa
       61 catgtttgac caaaaccagg ctagtgtctg tggtaacgga ggtcagactc attttaccta
      121 ctttatgaaa gagatttcct taaaactaag cctgaattac tgaaatatac ctagcttaac
      181 tggcaatctt gttttaagag tctatcagca ttaaacattt ccaattagta aagatcacaa
      241 gtaaacaaaa tttctttaca tcgaaataaa acatgcatag cttcaaataa atagagatta
      301 aattctaaca ttcacctatt tggtacattt aacgctctga gaacaaattt cttgtttttg
      361 ccagaaaaca atataattca agcattaatg cagctcttgc atccgtgcac gtgtgacttg
      421 cgtgattcat gtcctaaaca gcattttctt gcattgcaca tatccagagc ctcagcctct
      481 tcctctcctg tttttgtatt ttctaacatg acacatgaat ccttggtggc cctggaaatc
      541 agacaaccca ggtgcaacac taatatcctc ccagtatcca aacatttcct ttaagagtaa
      601 ctggcacact ggggcccata aattagagct gaacgctgct caagatgtct ggcttccaat
      661 aagaccgtac ccagagcaat aaactttgac attgctgctt gaaaaatgcc tgcccttttt
      721 cattgtcaag tgttacaaat tgatgaaatg agttcatcga tgttccctgg tcattatctc
      781 tccagtggca ctttgtttta cagacagtgg gaaaagggac agtgcagatt agaggcccag
      841 gcacagttta tcaaacaaaa tctaatatca tccataagag aggaatctgg aaatcaatac
      901 ttcattacta aattgttagt ggggatgaag agtaattgtg tgagttcaga gatgtgtctc
      961 ggcggccggg gcataggaag ttcaatgtca cccgggaagt gataagtaaa ggcactgatg
     1021 ggaagaaaca tttctgtaat ggggggtgct ggaggagggc agtggcctga gcacagaaca
     1081 gacaatagac actggtttgc cattgattgg ctcagctata aagatggaat ggttcaaagc
     1141 gccggagaga gaaagagggg gagacggagt gagagaagaa aaaaaaaaag agcatcacct
     1201 tctgaattta cccttgtctt gtcaccagtt tttgtgcatt gcgtaaagtc ttcttctgtg
     1261 gtcactccga tcaatcgtcc ggtacatttg catcctgaca gagataagag caatgataaa
     1321 acagctcctc tgcaaaaatg gaagaccgct tgatgacatg gccattaccg tcggctgaag
     1381 gtttgtcttt gaaatggttt ttgtgttaat gaacgttaat aaataacttt gatgattaat
     1441 cttagtcgtc gctgcacgtt ctgaatgttt acagcgccgc acatgcagct atctgttagg
     1501 tgtgtgatct cagccgagcc ctcgagctcc tcttttgttc acgctgtgtg tgaggtcaca
     1561 tcagtgcaat gagcacatca cctccgagca gcttggcaca gcctcatccc tctaaaagct
     1621 tgtgtctgga gtccctctga cctgttcccc tgcctcacca tgttcagcca tgcaaatcag
     1681 agtgccttca atttgaacat tcataagccg aacctgcagc ttgtgtttgg agccggtggg
     1741 agctcgcagg gggctcgctg cggcctgacg tggaatcggc gactgttaca ttttggtgaa
     1801 aggacaagaa gtggtgcaca ggtagagaaa aggttgtacc gtaacacacc aaacatg
//
LOCUS       KCVB01000604            1272 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28367pri ultra-conserved element locus
            uce-63, sequence.
ACCESSION   KCVB01000604
VERSION     KCVB01000604.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28367pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1272
                     /note="ultra-conserved element locus uce-63"
BASE COUNT          293 a          268 c          328 g          383 t
ORIGIN      
        1 aggcagtttt ctgccaagct gggagagcag cagagtgttt ggtcagtgcc agcagcaggc
       61 ctggatttca ccaatgagaa acgctaaagc aacgcacaca aagccaccct ctgttttgtc
      121 aattttaacg atgagtggac aaatcaaagt tggaagacag ggagtttttt tcttgtccca
      181 gtttattgat tttcaagtat ttcagcaatt ttaaacacac aaagctatat tttgaacgtg
      241 taataacaaa gcgtctgctg gtgcttttga aaaaagtttg actggtcttt gtaactgtca
      301 cagagtgtga tccattagct ctaattgtgc tgccatactg gttcagtcac atttgtcagc
      361 tggatctaag ctgggggggg gaacagaggt gcactactgg agttgctagc tagtagtcct
      421 ctgctctgac tgtaacccag tccagtcctt aatgatctat ctgtctacca cccgcacaat
      481 ggcccacacc gctgttcgct ttcatttaca gagtgccagt ctctgaactc ccctcatcaa
      541 ccctttgttc ctggctgttt catgtttttt ggggggagtt gaatggcata gctgtgtatg
      601 ggatactttt gagtgagtgg ttgagagaga cagctagtgt aggaattttc cagtgcaata
      661 cattatgaag atttcacttt ctgcctttga ttctgcactg acagttgtcc ttcagccctc
      721 ctttaaagtc tcactttcaa tccctaatgg ctgtcatgtc gctgcgctct ctggactctt
      781 actcaaagtg tcattggcat aatggtcggc agccgttggc ctcttttgat catccatgct
      841 gtgtgcgaga gcaactggag ccgaaacagt gaggtgtatg aggtgagtgt gtgtgtgtgt
      901 gagtgtgcac tgcttgcacc tctgctcggc tgccactccc aggcaaagct ccacgaccgg
      961 atatctaaaa ttcactttca aatagttttc ctcctgttct tggtgcctaa tagccgagca
     1021 gtggaaagtg tatgtgcatg tgtgtgtgtg cgtgagtgtg tgtgtgtact cattaaaggg
     1081 atagcacaga tttctactgt atcagcatct cagactcagc ccagtttggc actccctcaa
     1141 cggttatgca tactccaaca aagggatgaa gggccgcaag agagtgagag acagaagacg
     1201 agagagagtg aaaatgtgtg tgtgtgtgtg cgtgtgtgtg tgtgtgtttg tgtgtgtgtg
     1261 tgcgtgtgtg cg
//
LOCUS       KCVB01000605            1571 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28409pri ultra-conserved element locus
            uce-1055, sequence.
ACCESSION   KCVB01000605
VERSION     KCVB01000605.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28409pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1571
                     /note="ultra-conserved element locus uce-1055"
BASE COUNT          437 a          354 c          299 g          481 t
ORIGIN      
        1 caccaaataa taatgaaaaa aaataaggaa aacaaacaaa tctctgcctc aacctaaagg
       61 tcttgatcag gtgtgaagaa cacattcaat tacagcagca gtggccacag gaaatgattt
      121 cagtcaaaac tgttcctacc acaattaagc tgtgttaatt atgtatttca ttaagtaagt
      181 ggattagaag cagtgctgat gaaatcgtaa cagggtttgc cggaggccag gctgcagaga
      241 acagacagga aacgactgat atctctccct cttactacac ttctctgtta taccctgaaa
      301 taaccctata aatacttaac acctttaccg gcctatctag gctgtcttcg tttgcacaca
      361 atcacctggt ctctgtatct gcataccttt ttgtgtcgga tcatatagtg taattgtgcc
      421 atagatactg ttgacatcac atgctgtttg caagtcctct ggaaacgatg aagtctctcc
      481 atttcatcga agccagttca ttaccattca ggtgcaaaga ggagataaag caaaatgcag
      541 atcgctcttt ctgtctcccc ctctttcaca cccctactct ctcactcact cacatgcact
      601 ctttcatttt atctctctct cccttcctct gtgttctctg atgttttatt tgatatccca
      661 caataaagcc tctctttgaa ctgccatcaa ttgtgtatgg atttttcctc tgaggagaaa
      721 cagagagctg tctgaaaggg ctccagtccc gcaggctgcc ttcatattga tgcagaggag
      781 tattgccatc ttcattgctg gatcaatcac tcacgtcact gttgttttat ggctggggcc
      841 acttcacaga aagaagcctg acaacatcac cgcagaggtt tgcaaaacga tacagctctg
      901 atagttgagg aggttggagg gggcggtggg gggctgcagg agccttttgg aggcaagaga
      961 ggatgaattt agattctgta actgtcacac acatgcacac gctatatctc tcttctttac
     1021 agctgtggga gcctctggtt ttatcacttg gtgtggttaa gtcaaacaga aagcattgga
     1081 agaccaaggg atgtcgtcaa agcttgatgt gatctgtgtc accctatgga tacaaaatcc
     1141 tcctgcagcc ccccactccc acttcttgtc cgtctgttta cccacagtat attttgtagg
     1201 tggtgctaaa cttcctttta gtaccaaagg gttaaataaa ataaagggga tcataaaaac
     1261 agggcactaa tcaactattt gatccctaca catcatttta aaatattaat aattatctga
     1321 tttaaaggaa tattccaagt tttccccttt ttttaaaata ttgtccttca tagcttcttt
     1381 gtgtagacag tcagcccctt caaaatgtta acaaatgttt gcatttaggt caatttgtag
     1441 agtcattagt ggagtctctc tttagcacaa agtccgacta ggtcgaaact ttacagtgtg
     1501 tgctgtctat aatttttgct attttaaaac ttgctgacac aaaccaccct cttgtgttcg
     1561 ctactactta t
//
LOCUS       KCVB01000606            1289 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28494pri ultra-conserved element locus
            uce-366, sequence.
ACCESSION   KCVB01000606
VERSION     KCVB01000606.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28494pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1289
                     /note="ultra-conserved element locus uce-366"
BASE COUNT          401 a          187 c          256 g          445 t
ORIGIN      
        1 cttgtttgat tgtttgtttc ctgtaggcat tgtatgagct ggtgaaatgt gactttgaca
       61 cagaggaagc tttaagaaga ctaaggttta atgtaaaagc agccagaggt aagtagacaa
      121 ttaagtcctt cacatatttt taccttccgt ttttctgatc taatatgttt cgggtaatat
      181 agtgttctgc aattggatca tctgagtttt gtctttaaga tggtcttgca gatgcaagat
      241 taacacgtct tctctctgta cagaggagct ttctgtctgg actgaagagg aatgtaggaa
      301 tttcgaacaa ggactaaaag cttatgggaa agactttcat ttaatacagg ccaacaaggt
      361 gagagaagtg tttcaaagcc acattaatga cccttttctg attaatatgt catgaccgag
      421 taatgataac gctacattaa ttctgatcat gacattcatg ttgtatttgt gttgacgtgt
      481 ttgttgttac atggctgttg gatgctcagg gttgaaatca gttacatttc aaccatgttg
      541 cattaaagac aaatcaaatt tcttgtgtga aagtgtcata gatgacatga cctttgccag
      601 agttccaagc agaaatttga tttgtctctt atttgaaatt gtgctgaatc aatctcttca
      661 gacgatggta ataatggtta tttcatgtct gctgaattaa atctttcagg tgagaactag
      721 gtctgtagga gaatgtgtgg ccttttatta catgtggaag aagtctgagc gttatgattt
      781 ctttgcacag caaaccaggc ttgggaaaag gaaatacaac ctacaccctg gtgtcacgta
      841 agtttaaaaa aaaaatacat atacagtttt gaagaaaatt gcttaaacat tgtaaattat
      901 taataaaaat taaattgtat tcctctttca ggttgatgtg tttatgtttg ttgtttctaa
      961 tatttcaaca gaagtttcat gagtcaatac cacaatacaa atcatacatt aagctttgtt
     1021 caaacttgag attggcactt caagcaaata tactagtcga tttttaaccg ggaagtattc
     1081 gacttttcga catggcgttg gttaaaacaa cttttatcag atttatttta atatccatca
     1141 tttattttgg tgaaatactt cagtaaacaa atcaaatgtt tttctatttt tcttctagac
     1201 ttgagcagta aagaaatgac gttgtgttag tttggacaaa aggaatgtaa cagtagtgaa
     1261 gcaagaatgt aatctaagat aaaggggtc
//
LOCUS       KCVB01000607            1329 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28530pri ultra-conserved element locus
            uce-1311, sequence.
ACCESSION   KCVB01000607
VERSION     KCVB01000607.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28530pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1329
                     /note="ultra-conserved element locus uce-1311"
BASE COUNT          362 a          302 c          246 g          419 t
ORIGIN      
        1 aacacacccc acacacacgc agacacagac agacaataac acacacacgt acacatactt
       61 acacactcaa aaacacatga aatatctctc gaattatttc aattcaaagt gttgtaatac
      121 aaagcaagtt taaagctgtg gttttatatt tgggctatat ataggcttat attagccttg
      181 ggatgtttgc tgtgccttta atcctatttt tttttcttca gttcattaaa atcctaaatt
      241 attccccata tccattgtag tatatatttt gatgtatact tagagttttt tctatgctat
      301 ttgttggatg gatttgtaag gaagcactta taatttctaa aagaaacatc agtgaaaaca
      361 agtgattgac tgaactctgg tctcactagc tgatcctcct ttgggatttg cgttgaatgt
      421 gaaaatcctt ctattgaaat gacacaatgg gggagaggga gctcaggggg ggaaactgaa
      481 ggaattcacg aaaactaaac agctccagag ctccatagcc gtgaaagagg agatggtgga
      541 agaaggggcc taaatttaaa catttcctct ttgtcctgtt ttgagcccat tgtcatcgca
      601 tggcgttctc ccttctgcac aggaaggagt ggatctgaca tcaacgctct caggctgaca
      661 atgccaggaa gtctgtctga ctgtatgtaa atatctctat gtgtccacgc ttccttgctt
      721 gcccgtggag ccactctcct ctctctggcc ctgtccctgg agcggcttta tgctcttgag
      781 ggtgtagcag tagtagcgac atcgtgattt acatgctgca cttcacccat aactcatctg
      841 caactgtcac tgtgaattac agcaacctca tcttcaaatg acctctcttc tgatggagca
      901 tcttttgcac atatgaagac ctaataggct cttttttatt gaaatagcta cacacggtaa
      961 cctatatttg ttaatttctg cttttgtgtt ttttggttgc tctttgtact gtcatttttc
     1021 tttatagctt ttgggttgac ttactatttg tttttttcct tcagtcccat tccttcactt
     1081 cttcccttcc tccccttact cactcacttc ctcacaacct caagcagtct tcctgttgcc
     1141 cccccccccc ccccacaaaa gacatcctgg ctcaaacata caaaatgttt cagctcagat
     1201 gtggcttaca caggctcaaa actaacctct tatctcagaa ataaattcat ccaaacagca
     1261 ttttgtatga atgacagaaa gtgtgagggg gaaagagaaa gagggtgata aatggaggca
     1321 gacagatta
//
LOCUS       KCVB01000608            1361 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28577pri ultra-conserved element locus
            uce-1249, sequence.
ACCESSION   KCVB01000608
VERSION     KCVB01000608.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28577pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1361
                     /note="ultra-conserved element locus uce-1249"
BASE COUNT          354 a          305 c          328 g          374 t
ORIGIN      
        1 tgaggcctgg tttgagggtg cctaaacctc aaagtcatat aattcaaagc gtgtatgtca
       61 gccttgctct cattaccatg actgatcata ctgtctacca gtatcctgcc cgcctggcac
      121 ccaggacctg caggtggtac ttcatctaac gtccacgttg atttttcaca gaaaattttc
      181 ctctgctaag tcgtccgaaa tcacagcttg gcaagaaact gggagcaaaa acaacgcact
      241 caaatgaaga gttgaacaac ggcatacagc ttctgtttgt gcaaagcaaa gacaaacaac
      301 aaaaagcaat gcagttgttc tgggttgtat caatttgtta tttcatggtt agaagaaaag
      361 actattcctt ttcagaggta gtggacaata taataccatg aagtgtattt cgtgtatttt
      421 tttatgtatg ttattgctgt gagtggtcaa ataccacgcc acaattccat tttgacaaac
      481 cctgatttca tatatctaag tgctcaaacg cccaaaaatc atgcgtcacc gaccatcagt
      541 cttccctctc cggcatcgat ggatctttct tctttttttt ttgttttccc ttattctccc
      601 ccctctgtct cttgtccttc tcttttattg ctttgtacag caaagcacca ttactgatcc
      661 cttccaaagc acaaactaaa aatctatccc gttgtcattt gctgcaccgt gccatctatc
      721 atcgggggtg cttggcagcg tctccatacg agagaggagg tgctggaaca ggaggcagag
      781 tcaaggactt tcacgcgcaa caaaaaactt gtcacaatta tttatggctc ttgctgggac
      841 acataatagg acacatcaaa ctgctgtttt tttgtgtgat gagcgccctc acatcgctgg
      901 ctcccttgtc gaagtgggtc acaggcatct cttgccttac acatacaatt tccaagacaa
      961 tggcggagca cttggggaag tgacagtgac aaatactgga gcccccacgg aaagctgggc
     1021 aggctgttac atgagtgatt gcgccaataa tcatgcggag tcgtcacgtg tctgtggagc
     1081 aggagggaga tgggcagcag gggtggcagg gggtgggaag agacagagag agagagagga
     1141 gagagagggg gagtatatga gtgtgtgtga ataagacagg gaggagctgg gggagcagca
     1201 ggcctgtgta gagaagtggc taacaggtcc tggcaaatca ttttttattt ccttatcatc
     1261 catctcccac ttcctcccct cagatgagtt gctgctaaat gactgtcact ttggacggtg
     1321 ctgtgatatg ttctgctgta gcgcccgtaa ttgggaaggg g
//
LOCUS       KCVB01000609            1708 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28623pri ultra-conserved element locus
            uce-556, sequence.
ACCESSION   KCVB01000609
VERSION     KCVB01000609.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28623pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1708
                     /note="ultra-conserved element locus uce-556"
BASE COUNT          460 a          346 c          335 g          567 t
ORIGIN      
        1 gtcatgcttt gatctttgca tttctagtga ttaacagggt tttaaccatg cggtctgaat
       61 ctcatttgct ccatttagct aaattgttaa ttgttcttct ccttattgtc ctcctatttc
      121 cttaactata cagctgaggt ttaatgggtc tctggtgtat ggttttactt ttctggagtc
      181 cttggcatgg tactggtgta cttgtgcctg cctgtctttc tatttatttt cattttattt
      241 ggcatttcaa ttgaatgtcg aaatgataat tgtttcttca actgatgtct gtattcctgt
      301 ccatctgtct gtctctccag agaaatagaa ttaggcgtga gaaagacagg gagacagact
      361 ggcatgtaga caggaaaaca ggaagacagt cagaccactg aggttttagg accttgagct
      421 gatatttccc aaaagttttc actattatat cccgccaatt cttgttctgg tctagggttt
      481 taacacagcc ataggttcaa atatttttta atttatcgat ggtgtctttt tacttgagtt
      541 ttgaaattca aacacagagc aagagtagat catcactcca gtaatcttgg gcaaataaaa
      601 ctctagactc ataactgttc tttgttttat tatcatcata gataacagtt ctggccatac
      661 attcatctac ccatccctct gcctgtggcc gacactatac agttccatac tgtgctctgc
      721 ttcttcgatg ccttggcagc aaattaagtt aaagtctgcc tggaaaaaaa gaagccctta
      781 aatgttgcca tatgcaggat tgcatgggaa cttcctgagg aacacatgtt gttctgggtc
      841 tcttggagag gtgttgagtc tgtgtagtct atagagtgag acacagtatg gtggaccact
      901 caacaatgat aagagaaaac agcaattcat gatggagcat aatattttgt caacatgtca
      961 catttaagac ctgtcaattc caaacctttc agcatttgtt aaaaacagtg tgacatgtta
     1021 cactggagaa tcagctccaa cttaacactg agtgtcaaag tgctgacgta tttggacttt
     1081 tgacattttt gttgaatata attttcacca tgtctcaaat gggctgcttc tcttgcacgt
     1141 ctcaattctc agagtttgag aaacttaatc tatttgtaca attactagca cacttttaat
     1201 atctgaacga acaaaatgga gatttcagca atgtctcctt tcagggggaa ccagaaacaa
     1261 acatttctct aataaagggt ctctttcaaa aaaatcctta ccttaaatta acccttacat
     1321 attattacag tggagttgat gatgctaact gtgtcccttc tgttagctat attagatctt
     1381 taaaaccttc cgtccacacc tgtctgcttt ttcattgtat tgaacagcaa ccaggtgtat
     1441 cctgtcctga gcgttatgat gaaaaaatga gcacatactg tcatagaagt ggtgtcaaat
     1501 gtggtttctc ccttacagga ttactggttc tctctggtct tcaagcgtct ggttggtcct
     1561 cgggttttag ctgtgcgggt ggctggactg cagaggaagc cgcagccagg acgagtcata
     1621 cgagacaagc tacgcatcta cgcccactgc accagctact acaagtatgt gctacggtta
     1681 aacctgctta gattagttta taaccagc
//
LOCUS       KCVB01000610            1019 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28665pri ultra-conserved element locus
            uce-187, sequence.
ACCESSION   KCVB01000610
VERSION     KCVB01000610.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28665pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1019
                     /note="ultra-conserved element locus uce-187"
BASE COUNT          297 a          164 c          228 g          330 t
ORIGIN      
        1 agggacgtga ccaacttaag tacttgctga catgggaata agatcaagct aaaattccat
       61 gcatcattta tttgtgataa atatggcagt cggtaatttc acttctaaaa tgcattttgg
      121 ttgtgcacac tgtgaaaatt catgcttcat taagatatta aatgtttcaa tagggaaaca
      181 ttgtcctaaa gttgttatag ttttcctcct ttagttattt aatattattg tcatataatt
      241 attatgttat tttattgtaa aatattggtg gaagagtttg ggaactttca gtcaagtcaa
      301 ggggagattc caaaaaaaac ccaaaaaaat agtagcacgg gggagcatca tgctttttaa
      361 atcatgacac ataagagttc agcacccctt ttaagttcct tcattgtgct tgttcactat
      421 taaaagtcgt gacctcagat tttcttcatc aactgctgat gttgtaaaat atgtgaggat
      481 agttaaagga aacagaactg aaatgattaa ttttcttaga atgttaatgt ttgtgtctcg
      541 tggttatttt tggctttatg taaatcaata gatgatgttt taaccacagt gtaatttacc
      601 acttaatatg tcctgtcata acagaagtac agagatgtca cttaactgtc tttttgcaat
      661 tggagaatga gctaatcgtc acaaagctgg cagctgaata gagccagtga gtttaatctg
      721 accacaatga catgtttgat tggtcaaagc tggggtgggc gggtgccagc ggttggtgtg
      781 tgaggagggt agccttctcc cgtggcagtg gagaacaaac ataaatcttc tttaacaatc
      841 tattttccat catacttatt cacaggggac atctcaaagt ggtctttacc ctttctgtca
      901 atggctgccc tcatccatca atttcataga gaattatacc ccagatcgat gtagttcacg
      961 accgctgagc atttgtcgag gggtggtgga ggtggtggag ggaagggggg ggggggggg
//
LOCUS       KCVB01000611            1510 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28707pri ultra-conserved element locus
            uce-513, sequence.
ACCESSION   KCVB01000611
VERSION     KCVB01000611.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28707pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1510
                     /note="ultra-conserved element locus uce-513"
BASE COUNT          480 a          278 c          317 g          435 t
ORIGIN      
        1 cagattgatg agtttgttaa atcagtttaa atttttagta tttcataaca aagaaatgca
       61 aaaaaaaaat acgtgatgga gttggagaac tggtaaaaat cctccaatct taatagccaa
      121 ggagaaaata caatttgggg cctgaagggt agataaaaga atttgtggtt gggaaaaaat
      181 tcagtgtatt acagagggaa atgctccttt aacctgtagc tatgagaact agaagtatgc
      241 ccttcaacac ttggcagttt gctttcatat tctaaatctt ttcagtggag caaaaggctt
      301 ctggacttta agtccgagtc catcactttc catatctggg gaggaggaaa tgaaaggtat
      361 tggaggaagg caaaggccag aaggagaaag tcaaagggat tatcataaat ttacatgata
      421 aagctctccg ctttaaatgc aggaagtttg gaaatctgtg aagagtagaa aacaagtcca
      481 atacagaggc atgttgaaga gaatctacag catttgatgt ctatttgaaa aacaatggaa
      541 agctaaaact ttggcatttt catgagtcaa atcccacaga atatcctggt atatcaggtc
      601 agcttgagat gtttgacatt ggccagaagg aggagaagga agtgctgttc tgctttggtt
      661 caacaggcca aagacagaaa gccagggaga agcagagaga gagagaaaga ggagaaaaag
      721 agcctctcct ctcatcctca ttatgtaaac gaggtgcatg actgttcagt gggtccctcg
      781 agagttaatt accagtggct tctcagccta attggctgaa actaaagaat ttgccatttc
      841 ttattcccat tccccctctc tctctctctc tctcttattt tgtctttctc tttgtttctc
      901 tctctttgac acaagcacat ttccctctcc ggcttttcaa cctgttttct tgttttctcc
      961 tctctccctc ctcagtgtcg gccttccagc tttgcttcat gccagttgaa aatctggcac
     1021 tgatgcttaa tttgagcatc tgctccagct ggcagtggat aagcagattt ttacattgct
     1081 aattccagct atttggctag actaatcact actggataaa gaagagaaac gggggctcag
     1141 agttgcccat cacttagaaa caaaaaaaga gtagtgagag ctggcatctc atctatttct
     1201 aaattgatgc aaattaaatt caaaaagaca gggataacaa gtttgaagta tatgaaagtg
     1261 ggcaatactg tgaggaaata ttaataaaac agggcaggca aggagaggaa ccgagggaat
     1321 cagaaggctt tgattttcat aggacagagg aaccaaaaag ctgtttttgg acatgtcaca
     1381 cacatataaa taaacatatt ctctttctta ctgtctttca ctctctttac tccaaatgta
     1441 tttgatgtgt ctttgcaaat gatcaaagtt aatacattca tcaagagact gcagcctcaa
     1501 ctgtcatatt
//
LOCUS       KCVB01000612            1495 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28748pri ultra-conserved element locus
            uce-1148, sequence.
ACCESSION   KCVB01000612
VERSION     KCVB01000612.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28748pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1495
                     /note="ultra-conserved element locus uce-1148"
BASE COUNT          424 a          311 c          328 g          432 t
ORIGIN      
        1 ttgtggtttg caaggacaca aactttaaaa acgtttttgt ggtgaccagg ctgcaaatgc
       61 atacagtata ggcctgttgc tttgcatatg cagcatgtgt gcatgtctgt gtgtgcatta
      121 gtttatatgt gtgtgtgtgc ccctgtaagt gtgtgtatat ccagtgcccc tggctaagtc
      181 tctttagatc ttttcttgtt tatgcatttc tcttcctaga ctccaggccg caggaatatg
      241 ctgggggcta tacagacaca gaaaagtagg aggaacattt tatttattta ttgtcaggaa
      301 ttatcactca gcataaatat caatacagcc aaaggacagg aaggttggag ttctgatagc
      361 atggaaaatg tcggagaact gcaactggag atgcagaggg tttgatgcgt gatactcata
      421 ccttatggct gtaatattac acagagcagt gtgcttacag cactatgcac cgatctaaat
      481 agagcaattt aaggtttaaa ggttaaattt agataagaac tcaagtacat ttgccaaaga
      541 ctgagttttt tatgaccatg aagctgcata gtttgagtct gttaaagata tagattgtat
      601 ctggtggatt cctacaagtt aagatatcat caaacataat ttgggtcctc agcaacagaa
      661 aatgattggc tttattttat gtgctattta ttatttcagt cagttccatc cataaatcaa
      721 aataaattat ttaaaatcat tactgtagct attacgctgc acattacaca tctcaatgac
      781 tcatattctt gttggtcatc acacgtgtta ttccagctcc tcgagttttc aaggaaaagg
      841 catcattgag gttaagctac cgtacgtaac acagagcaca atgccatcag ataacttgtc
      901 agtcacgggc agctggaaaa ggcacataca cacaaacgct cacactaaga tgtgccaaca
      961 catactctga gtgcttctgg atagaaacag agagtgacag tgatagaatt tattgtgatg
     1021 gtatcagcaa agcagccatc acagctttgg cagattggaa ggccatcagg acccatctcc
     1081 ctggctctga tcagtgttcg catcctgaca tcagcctgcg atgttaactc caggccctga
     1141 aagggggcta tgaaccatga tcagcaatat caagccacca gaaaatagaa atccattttc
     1201 cggcctcgag gagaataggg ggtggtgaat aaatatcact ggcctcctgg tctggtctgg
     1261 tagggtctta gttgggtgtc tgtctctctc tctctctctg cttcaccacg cggggccctt
     1321 cagctgtttt tccctctcct cttgttctga aggttaaagg attaggagct gtgttttcag
     1381 accggattgc tgtcctggca tggatttaga ttttggccac acacactaga aactagagcc
     1441 ccaacacacg cgcaggtgca catatgtaca tgcaaacaca cacacagacc accag
//
LOCUS       KCVB01000613            1186 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28790pri ultra-conserved element locus
            uce-286, sequence.
ACCESSION   KCVB01000613
VERSION     KCVB01000613.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28790pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1186
                     /note="ultra-conserved element locus uce-286"
BASE COUNT          346 a          265 c          270 g          305 t
ORIGIN      
        1 caagcttggc tcgtgtaccc accgtaatgg aaagagagta aaaaaaaaac acacacacac
       61 acaaaagttt gctgtttgcc ttcattccac caaatccttg acagccgtgc tatagcccat
      121 cactattcac accattgtgc tgtcacaaca actaaacaca tcatttgtgt tgcagaaact
      181 catcctgtat gtatcaaata ccaaatgttt ggcgactgag agaggcgatg gcggaggggg
      241 ctgcataatt tgctaggaaa cattttaccg aacatttggt gataaaaacg ccggcgtcgg
      301 ccccaaggct taaagcaatt aattcaggat cctgacataa actctccaat cctttgacag
      361 cttggagctg aaataaaaat gctagttctc ctgggaacgg caggagatag ctggctcttt
      421 tctggactgt ttgctcactg gccctgagag caatcttccc cagtcagaat gaaatgcaag
      481 gacacaaaca atgtaccggc gagagaatgg gagatgggga atgaacagtt tggactattt
      541 gctcagcaga agggcagcca caatggccgg cccatggctt tcccatgcat caagcctctt
      601 agtcttaggc ctccctttgt tgcctggttt cttcttccct ggccagataa gagcccagct
      661 gccctatact tttaattccc attgtacacc atactgtatg gtcatttatc tagccacatt
      721 ctgcctgcca tgacggtgtt atctaattga atgggttatc gcgtgtgtat ctggttcacc
      781 ttagcatgtg tactcgttta ggggttgaat cgctctcact ttgccataaa aagcagggtc
      841 ggggaggggg ggataacggc tgacccctct tctcttctta gaggaggcag cacaagtcaa
      901 gcacagagtg cgataacgat acccaagagg ttttttgctt aagatggcat tatcattata
      961 gagcagttag aataattttt attatgataa gaacaaattt aaaaagtaag atttctgatt
     1021 caagaaaatc taagagagga gccctttttc tagaacccga caaagatgta cttttccagc
     1081 caaaggcaga aagcttcaga gggcccttga gggagaaatg agatgcagtc attatatgac
     1141 ctaatggcaa ctccagccag aagagagggc aaacagagca agtgta
//
LOCUS       KCVB01000614            1603 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28832pri ultra-conserved element locus
            uce-657, sequence.
ACCESSION   KCVB01000614
VERSION     KCVB01000614.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28832pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1603
                     /note="ultra-conserved element locus uce-657"
BASE COUNT          423 a          356 c          395 g          429 t
ORIGIN      
        1 ttaacaggga acagcatttt gttgtttaat caggggtaag atgtgcacca ctgacaaagg
       61 gcatacagta ccatcggagt aacatttacc atcagtcaag ggatagatca ctgctgagct
      121 caccatgtgc ctataggatg gtcgatcgca atgttaacct catcttttac acaggtcccc
      181 ccacttaaga acgctccacc attgtaatga ccactctgta gtgagtcaca gtgggctgct
      241 atgtcagttg tcaattcggc ctgcaggcta caaaagctga tacagataga ttggtcacac
      301 gccaatagcc ctgtataaag aacagtaata cacactcgtg ttgattacac ttgagccaag
      361 tacatccatg tactctattt cagttattca gcccaacaat ggtgcctcct gaccctgctg
      421 gggctaatga tagagctggt ttggtgaaag agggacacct accctccttg gcctctctgc
      481 ccatgcccct gctctgaggt agggggctgg gatggtttga catgcagttg agcaggaggt
      541 tgcctctggt tggcagccat tgtgtaggat ggcattatgt tttagctacc cctactgagc
      601 acccctcatt caggtaccaa aaaggtctag gtgcttagct acaggggcta gtgcgtcggc
      661 taattaggat catatacagg gaagcttctt tatctcagtg ttcctctttc agggactgaa
      721 gaaaggagga gagaaggggg ctgcttggtc ttaaacaaca ggatctgtat cacctagaga
      781 gcaatttgtg ccaattatgc cttcaagtga aacggagcaa tttaacagct agggtatcaa
      841 catggggctg tggacacgtg caagggggag tagcctgctt aggagttagt gtggtgcatg
      901 tgtgtgtgta atgtgctgag tgtgtgtttg cgtgcatgta tgtgtgtcag tatatggggc
      961 ggggctgggg gtacaatggt gcggtgcagg tgtagtttcc tgctgtcaaa atgtctgtga
     1021 gatctataac ccattcagac gccacaaccg taccaccatg tcactctcac tcatgagacc
     1081 gtcgcacaat gctgcaaaca tgaatggaga aaatatttta tttggtccct aataatacgc
     1141 tcaaaggcac cgtttagttc tggacaaata tttaggacaa tagcaaatgc tgtccaattc
     1201 attgtccaca cttgtgaaaa tgtatacttt aacctgaata tccacaacac ttaatgtgtt
     1261 tgcatgggtg ctccgttaaa ccgtctctgt ttgctgaaat aaaggaggag ggattctata
     1321 aagcctcctc cacatcctct gtgctccaga ttgctgtatt tacccagtgt gtgtgagtgt
     1381 gccttcgtgt gccctgtgtg ttcagcacca ctctccgagt aaagagcctg catgacctct
     1441 gaaccctaag tacactcaac gcaggagaaa tcaaacagac actgagcaaa caagaccctt
     1501 atttgaatag acatacacaa cctttcggag aggctgcatg tcttaggcca atggcaagct
     1561 actcagaata gaggatgaac ctgatttgtc attagcaaag ggt
//
LOCUS       KCVB01000615            1250 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28873pri ultra-conserved element locus
            uce-1335, sequence.
ACCESSION   KCVB01000615
VERSION     KCVB01000615.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28873pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1250
                     /note="ultra-conserved element locus uce-1335"
BASE COUNT          305 a          323 c          255 g          367 t
ORIGIN      
        1 aatgatttta cttttaatct cttgttattg agaccagctt ttttgtttat aagggtgggt
       61 ttatgctaag tggtgaccta acgatatttt ggcttcagtt gtgcagcaga atattttatg
      121 ccttatgata atctgtggca ccaatgtagt ggttgaggat catacattat gcagacgtta
      181 tgataaaaag cactgtagta ggacactctg tggacattac caccctccgt tctttacaca
      241 acctcattga cacacacaca cacgcaagta acgcacatgc attcaaattc acacttacta
      301 acataactca tatcacactt actctactat cctgctgtct cacctaagca gtgctgattt
      361 tatctaagcg cacagagcaa gtaaacaaga gttacctagc agcagctcct cagatgaata
      421 ttgtatacac ctagtctggc taagtgttgt tgtttatgtc agggacaggg tttatggtgc
      481 ttggtgggcc tcttagtgca gttaagtttc tacagcatcc cactatggcc atcaatgtca
      541 gtacaagatc gtctaggttt gtgtttttct cccccctctc ccccctctcc tcccgctctc
      601 ttcttctttt cccttcttcc gtgctcggct ggtgttgcct gcctgctccc tcgtctctgc
      661 ttgcccatct caccttcctt ccttccagat ggcgcatttc acaacctgct aggcacgatc
      721 aatcaagccc agatgcagac tgggctatta aggtcagtaa tgatcggcct ttgagcgtta
      781 tgggcccggc tcctgttgta aagccatgca tcaaactctg ttccagcact cagctcgcct
      841 gcccttaaca agccagctta aaatatcggc cggaggatac aaaatgaaaa gggtacaacc
      901 ttatctctcc aaagacaggc cgctgtagcc ccaaggtgta ataaaactac agaacttggc
      961 cgtcgcctgg agtcatctct tttataagca atatctttca aagatcagct aagcggtggg
     1021 ctatgggtgc tgggttgaaa gatgtaatgc atcacaccat attcctctgc acaggaaaaa
     1081 cctctgctca ctattccagc ctgttcatgg gagctaggtt cttccatcct tgcctcatac
     1141 cccacgtggg atttaggttt aattgtgtga gaagctcagg gtttgaagag ggatttgaca
     1201 ggcacctgaa ccctgcccct cagcccgctc tgaccatgta caaatgcatg
//
LOCUS       KCVB01000616            1365 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 28953pri ultra-conserved element locus
            uce-793, sequence.
ACCESSION   KCVB01000616
VERSION     KCVB01000616.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="28953pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1365
                     /note="ultra-conserved element locus uce-793"
BASE COUNT          398 a          265 c          318 g          384 t
ORIGIN      
        1 aaagcattgg attcatagag tggcgccaat gttaccactc tgcactatac tgtatgtgta
       61 tcagcctgca ggcttgcaca cttgtgctta tctgaatgaa ttgtgcatgg ttaattgagg
      121 tgtgttttac ctgtaaacag gaatggaaat agccagacag agtaggtggg atcctgggtt
      181 ttcacccagt gtactttgca gttgatagcg tgaatgtgtt aatcagaatg aaagaagctg
      241 taaagccaag gtacaaatgt ctccgagtat tcaaagaggt gtagtcctta ggcgtctata
      301 accaagagaa aaatggcagg aatgtctaga atgtagttgg taaacaatgt taacaaagcg
      361 cagtggactt tgtaaagaac atatatactt tattctcttt ggctgggtcg taaataatca
      421 aagtggtttg tggagaacgt aaacaacatt gtggtttttt gcagacacac tgtgttgtgt
      481 aagatgtaat gtatatttta ctctggctgg gaaaggaaac ataatgcgtt tagtgagatg
      541 gtaaagtcag taagcagaaa aacacatgct gcagcataag taaacaatgc ataatgctac
      601 attgagatga gcaaacaatg ggcacgtgct gattcgcagt caacaatgca ataccgcacc
      661 ttaatgtgtc tgggccaaac aatgcaaaag ccgattatgc aaattggggt tatcgagggc
      721 actgcaatgt aacaatggca ccaagttgat tgagctgggc tgtgtgtgcg tgtacactac
      781 ccatcccctt gggttccttt tttccccaca gatgcccttc ccaaaaaccc cctatcttat
      841 gctctgttct gaggcctcat tataggcact gctgtcaatc agagggtcaa gggtcatggc
      901 agaggatcag cttgatcaat accccccggc ctgatcaatc aacttgtctt ttcgcttcca
      961 ccaatgagat accggcttcc atcaaaacaa aggagtgaga ggaaaaagaa tgccagaaag
     1021 aattaggagg agtaagaaaa gggagaacgt aaacaagcct gcctcgctga acagatccca
     1081 gaagcttctt tttttttctc attttagaat gacattgtca aggactggga cattgatgag
     1141 cggcgtgcgt catcaggatg cacctgtaat gcccttggga ccctctgtgt ttgctccaga
     1201 tctgagacac cttgtgagag atttagatcc gtggctgcat tgtgatcggt tccttatggc
     1261 aaaaacacaa acacatatat acaaacacac accaaacata tatatctgta ctggtcgtct
     1321 ttgtcttctt ctgtggttgc tatagttttg ttttttttta agttt
//
LOCUS       KCVB01000617            1988 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29033pri ultra-conserved element locus
            uce-1034, sequence.
ACCESSION   KCVB01000617
VERSION     KCVB01000617.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29033pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1988
                     /note="ultra-conserved element locus uce-1034"
BASE COUNT          574 a          422 c          335 g          657 t
ORIGIN      
        1 caacttccca tagcccaagg tggtctctta aaaatggctt gttgtgttaa attaacagtc
       61 caaaatgcaa agatgttaat taaaaatgat atatgagaaa gacaagtagc aaatactttc
      121 ttttgagagg ctggaaccac aaaatatttc accacttttt tcacaatgtt tagtgaaaat
      181 gacttaaacg atgaattttc aaagtatctg ctaaatattt ttctatgcag tttgtaaaat
      241 ctcagatatt tctcttaaag ttttgggtgc ctcctaaatc cggaagttca gggtgtatgc
      301 taatatgagt ccgtcaccta aaaaaaacca gagcaagttc ttcatttcct gatcgctctt
      361 catctttgac acattacaac tgacaatggc tgcgctgagg ttatccatta tccagtttgt
      421 aaaatggctt tttcccagtc tctagaatgt ctttcaaaac agtgtaacag aaagtgtgga
      481 cacgctacac cttccctgtg tcttcatttc ttcgtccctc actctgtcct ccatttctgc
      541 ctttttctct ctgactctct cactgtgtga ccttgtgttc cttctgtgtt agtgtgtgga
      601 aattgggcac acctaactac aggggcttaa gtataatcat gtatttagct gcagaaatgt
      661 actgacgcac tgttcctccc tggaaaaaga gaactcccaa cattaattga gggatattac
      721 tgttgagatc aatttccatg tttctgacac tgatattcat tccctcagac ctgaacaaat
      781 atttatgtct ggttgaaaaa aagggaaaat gacaaaaaaa aaaaagaaaa ctattcattt
      841 tcattcatgt ctcatttgag acaaatttta aattacttgt aaatgagata tatatacaat
      901 tttgattaca aatactgaaa agtaaataat tcaggtataa tttttgtagc atttgtatgt
      961 cattttcttt cttttttaat cattttttaa aaatgtaatt attaaaaaaa atcataacac
     1021 aattaaaata acatcaagta gcctgaccat ctgcagaaat gcaattctgc ccatttgcat
     1081 gcattacttt agattttgtc tgagtggtaa gactgccaat aggggccagt gtgtaaccaa
     1141 gtatggggcc tcagtgaggc cgtttttcat tggtggaaga gagagacagg ctaaggggag
     1201 taggtgggga ggacagaggg ggaggctatt gttggagggg ttattgtgaa ataggaaacg
     1261 gcaaacaagg cggtctttaa ctctgcttat agaatatgtt aaagtgatgt ctgtgatgcg
     1321 ctgtgtgaat tatttgcctt atcataaagt gcatttatga cgcacggcca taaatatgca
     1381 tcagtacgag tgttttcaaa attgaacctc tcccctctct cattcccctc cttccccctt
     1441 tctccctccc tccttctttt tgcttcgtgc taacaacaca aacacaacat ttttaaaatg
     1501 caacccacca gtggatgttt tggaaacatt tcctattccc tcccgctact ctatcatagc
     1561 tgctctttct ccctctgtct gtttctcctc ctctcttccg gtaaatctgc ttattgtatt
     1621 ataatgtggg atgatttgaa aaatgttttg aagtcacaaa gtaattagaa aattccacga
     1681 attggtacga ttgtgtaatg gaggtactac aagtacacac attaactgct ctctcgctct
     1741 cacccaccct ccctgtctct ttctgtctca tcttcatcat caaaatcctg tttctaattt
     1801 ctatggattt cctgccaaga gcagccacag cgatgacccc cttaactcgg acatataatg
     1861 ttcttaatat ccagattaaa attttttctt taagctcttt tctacaacat gtgaaattat
     1921 ttctgtaatc aaccacaaag ccaaccccgc ccacaccctc tgtccttccc tttcccctcg
     1981 atcatggc
//
LOCUS       KCVB01000618            1121 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29068pri ultra-conserved element locus
            uce-492, sequence.
ACCESSION   KCVB01000618
VERSION     KCVB01000618.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29068pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1121
                     /note="ultra-conserved element locus uce-492"
BASE COUNT          343 a          279 c          206 g          293 t
ORIGIN      
        1 ctctctctct ctctcacaca cacacacaca cacacacaca cacacacagt ccagtcacaa
       61 aatcatacac acagaagtgc aaggacttgt tccataccag caggcacata ctcatctcac
      121 gcctcactgc actcattcat atcaaaagcg catgctcatt acgtacaaca gcagaacaca
      181 cacacacaca cacactgggt tcatccaact tgacttctct cataaagaca ctataataaa
      241 catttgaaca atatctctta gcagcggctg cttatgaatc cattagctca ataagtgcat
      301 ttcttgtttc attataaaat aactgtttca aaatggctta aggctaaatc tgccatatgc
      361 aaaaaagcaa tgaaaagaga cagctctgct ttaagcagat ggttccaact aacagctgga
      421 ttcatgtttc ccgttcatac agacattgtt tgacatcgtg attattaaac aggcccattt
      481 cagcgctgtt ctttctgtgt gttggtctga tattggtatg cagaggcggc cacgggagga
      541 ggaggagtgg acggtgcgat gagaggatag acttggatgt gatgagaggc tgagggcagt
      601 cagtcagtct ggacgtttgg cacagagaca gcagattcca tttccatgcc aatctgcctt
      661 gtctctttct gaatcaggct tgaccttcac atgctgatga ctctagaggc ctgggaccgg
      721 gagacgcttt ctgcctaaga ccagatacac acacattgag atatgttact gcacaaggca
      781 tgcacaatgg gaaaaacatt cccctcggtg cactcctaac tatatttgca cacatgcaca
      841 tgtagtcata agtatacaca cccaaagccg atgtaaaaaa gtctctccct tgatttactg
      901 taaaggagtg atacaaagga ttgaaatcag accagatcgt ctttagagag ccaaccctca
      961 gagcagatgg ctctgttttt catcttccct ctcatgagat ctcattttct gtgctctgtt
     1021 cagcaatttc actgtttttc accatttgag tttgtttcca taaaacacca acagaatcca
     1081 gttcaacaaa acaaatacca aacaaaacaa atgactctcc a
//
LOCUS       KCVB01000619            1667 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29152pri ultra-conserved element locus
            uce-673, sequence.
ACCESSION   KCVB01000619
VERSION     KCVB01000619.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29152pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1667
                     /note="ultra-conserved element locus uce-673"
BASE COUNT          462 a          400 c          387 g          418 t
ORIGIN      
        1 actgctttct tatagcatct aactacaaag ctaaaaaagc aaagtcctgc agcaactgag
       61 acaacacgta gccttgcctt tcaatactac accccctgcc ctgcagaaaa cacagtcctg
      121 gcagagaggg cttaagccct cacacgagtc tggctttgcg gcctctgttc ctgtgagccg
      181 gggaaacttc aaagtgaaac ggaaatcaag cgcacagaca gccttctgtg atttctgtgc
      241 ccattcaagc tgctttacag agtaacccaa cacctgtggg gaggctcaga gaggctgaga
      301 gttgaggtgt gtgtgtatgt gtgagtgtgt gaatgagaga tggacaggga gaaaaaggtg
      361 tcggtgagac tacacgctgt atggatcaaa ttctgctttg gtgttttatg attctacgag
      421 gggcttcgtt tgttacgcaa taaaaccttc agggaaaata aaagttgaaa cacattcgct
      481 ggcatgacat aagcataaag tcaaacactc atgaatatca ctcaagatga ccttatcttg
      541 tcttacaata gacagtgaaa gtgagaagag gagcagcgtg agatgtcaat caggtcttgc
      601 acacaaagac cactcatcac ccactagaga aacagcacac aagttcaaaa caggaggccc
      661 agtgattaat aataacttca ttaggcccaa gctgctgtag cctgtccttc agagcacttg
      721 agggaggtct tctcatatgg cttcctgcat cagatgagtg ctatctattg attatattgt
      781 actgggcctt tttaatggtg aaaaaagtgg gctatttaca gcccagctaa gtctgctctt
      841 cctgctaatc taatccccaa gctactcatg tggtcattcc ttccaagaaa gctgagagct
      901 ctgtgtaact ttatacctgc ctgcccctaa ggagagagag aaagagaaca tgaaggagag
      961 ggaggaagag ggtgagtggt gtgagtgggg agtggggtgg gaagttaaaa gatgggggtg
     1021 gggggtgtat gaggtgaaat cacctttggg tatcatcgtc acaaaccctc ttctcttaaa
     1081 ctaaccctgt ctgccctctg ctcaccccta acacccgagg ttagttcagt tctgctccat
     1141 taactgggcg gctgtatccg catcttcctg taaaggcgag ctgcgtacat gtacgtacac
     1201 atgacctgcc ggagatgtcc gtgaagctga aagataaact catctgtttt cacatgactg
     1261 tacttgacaa tgctgaaaca ttaagagtgg actgacaaca gctaagacgt gccacaacac
     1321 gaaatgcatt aaaaatgtgc ggcacactag cctacaaaaa caggcagctt ctttacactg
     1381 ccttcaatca taaaacgaga tacagcacac aaacaccctc tgaaggagac gctgcgagac
     1441 ccccctaagc tactgtttca ccgtgtgcac tcacactctc caccccatca tccccctctt
     1501 tctccctttc tctctttctc tctgaggact ggactgcctt ttgttcgcag ggctctgaaa
     1561 cccaagccgt ttcggccggt aacatctttc actctccggg cctcactgga gcattagctg
     1621 caactcgata tgggtctttt caaagcctcg tattattggg taaatat
//
LOCUS       KCVB01000620            1916 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29193pri ultra-conserved element locus
            uce-636, sequence.
ACCESSION   KCVB01000620
VERSION     KCVB01000620.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29193pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1916
                     /note="ultra-conserved element locus uce-636"
BASE COUNT          530 a          363 c          376 g          647 t
ORIGIN      
        1 gatcatgagt cttgggaatg gttggcataa tactagaggt ctctaatatc tcatatgtct
       61 cataggactt ttaattggat tcaggttagg tccccaagta gagttgtgca cacagcccag
      121 ttctgccaca gcggaaagtt aatcaaattg aaagtagatc actgcagcgc gctgcgacct
      181 gctaacactt taaaaagttc tgttccagat agttagacga aaaagtttac cgagtggttg
      241 ttgtggtcaa aacatcagaa caaatagttc ttagactttg cgttcttgct gatgaatttt
      301 gtttatcccc catgaaaact attgatcaaa atctgcatcg tgcaactttg gaaaaccagt
      361 tatggcctat atctttgtgt tactgttgtg agcaatattt taattgtcca ttttaggctt
      421 atcctggctg actcaaaagt aaaatactga atttgggaaa gcttggagaa agctttaggc
      481 attctacagt ttgtgtttct ccatattttt gtgatgcatt aacaatggct gctgcgagtg
      541 tgtgctccac agcaatatac caagggacag atcaaggtgg ggtttggatg aaatggcatg
      601 aagtaaccaa atgtgctatt ggttcaattg gcatttatca acgttagagg gtggaagatg
      661 gacttaattg acctttttta tgtgacattt cacattaaat taagcactgc actcatcagt
      721 cttcatgttt agcagttaag cagtcctgtg ttgtcgtctt cacaaaatat ttcagtgcac
      781 agtaaagaga aaagtttccc ttaaatcccc caggctttag tcttggattt gtgttgttct
      841 acaagtctgc gacatcatcc gaaccccagg agcctcttgc tcagtcttga cggatcctca
      901 ctctgctgaa gcccaatact gttcttcatc ttgtgttgtc acaacccccc tgctaccttg
      961 attcactctt ggtcgttggc taggttttgg atttattact ttcgaggccg aacaatcagt
     1021 ggaccaggct gtcaacatgc attttcacga catcatgggc aaaaaagtgt gtagttgtag
     1081 ttttatttta cccttaagat cagacagagg cttgggcgat gggtcagaat tatactatga
     1141 tgctcctttt ttattagttc aagcacattt tccggtggct tccattcgtg actgtagcat
     1201 catatagaac aaagttagtt tttaacatgt tacattgatc aggcctctaa attaatttcc
     1261 ttttcttatt tttggctcaa aacacaacct ttactcttat tgctatggct taaagtttcc
     1321 ggagacaaaa caatctgtca caaatgctgt tatattacag tttctattat cgcccaaggc
     1381 tttgtttaaa ttgaccactc ctttaggttg tcttaagtgc tttagattgg acttaacacg
     1441 caaaagtttc aatattatca gttgatttgc agaggcaaaa gaaaaaatta tgtaaatatt
     1501 ttaaagctca aataattttg ctgaatatga agcacctaaa ggattgggcc aatcagttct
     1561 gtttcttttg ttttctggta ttttgttggc aggtcactta cacgtcaaca tgtttttctt
     1621 ttttttcttt ttgaaaagtt aaagacaaag ctcaactcag tgacctcagc tgaagaatct
     1681 ccatactgtg tctgtcagtt ctctaaataa caaatggcag ttctctctaa aatgctgctt
     1741 aggatgagac atgagtcgca caataatatt tgcacatcac ttcttagtgt ttgttctatc
     1801 tcactgtgtg taccagatgg atggggttta ctgctcaaag acaattcaga tagtgtcagg
     1861 taagctgtcc atccgggaaa aaacaataaa ctcactatgg tgttatactg tttatt
//
LOCUS       KCVB01000621            1631 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29230pri ultra-conserved element locus
            uce-958, sequence.
ACCESSION   KCVB01000621
VERSION     KCVB01000621.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29230pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1631
                     /note="ultra-conserved element locus uce-958"
BASE COUNT          521 a          302 c          277 g          531 t
ORIGIN      
        1 tttgtagttg ctaacaagga ttcctttgga aaatctcttt cttagggaag ctttgacatg
       61 gaagatttaa gagttagctg ttatcctgaa tcctgatgct taataaagct aatgagctaa
      121 caggattctc tgcaaattaa actttttttg tgtgagagga attcatatca atttgataaa
      181 tttgcaaaat caattgagtg catcctgttt cctaatagat caaccagatt tgatgactga
      241 atagaaggca tccttcattt tctattgccc attaaaatag atatagagca tgataactgc
      301 aaacaagagc cttccttttc cgactaatac tcatgagtac cattgtgctt atatcctcga
      361 tatattaatg ttgctgcctc cagcatcagt tttaagtttc ccacaaggac cataatgcac
      421 caaaaagtct gatatcagct aacagagaaa aaatactgtc caaatacatg ttggcaaact
      481 catatatttt taaatgttct ttttttcttg gcagaaataa cttgagacag ttgaggaaaa
      541 aatagcaaaa acacagataa ataaaaaaaa tgtatgaatt tcgcttaaat atttctcagt
      601 ttgctcatcc tgtgatacag aaggaccttt gtccattaaa gcaaagtgct tttgctgtat
      661 tttttgagcg agcctgtaaa tgtccacaga cacacaaacc ccgagtcaga cacacaaaca
      721 cacgacctac atatactatc tggccttctc atcatttatc ttacagcggc agaatcagga
      781 tagattggtt caattagact ggcctttaat gtaatctgtt taatccattt ctatcatcaa
      841 agccaatacc tacctctctc cttggagagt gggattagcc ctccaagtgc atctagaagg
      901 gagcgtctcg gctataaaaa cgcagcctcg gagtcactca gatcttctcg atatttggtc
      961 tttacaactg gctgtggaga aaacagagtg tttcaattga agttttaatg ggtaaagatt
     1021 taagatcttc atatcctacc agagattgca aacactgttt ggcacaatgc acagtgccac
     1081 aataaaaaag agtatttctt cccttttctc cttaaagatt gccaagttga tctcatattt
     1141 gggacagaaa aaaaagattg ggttgcatca gctattctca atttttcatt cctacacaaa
     1201 atttgcatgt gtagttgttc aaaagttgtt agtaactact agtttcaaac tagcggttta
     1261 ctaaattggg ttgcactatt aaaactgaaa tgttttagct ggtgaagact ttaataataa
     1321 taaactgttc aggcttggat acatttgtgc tgccattcca atcgaagcaa caatagcatc
     1381 acaagctgga acataattac ccataacaac agttttacag ggtcaaatga tgaattaact
     1441 gatctgccaa tcctttgtaa atgtattgct ctgctttatt aatttgcaca catggatgtt
     1501 tcagagtcaa tagaattcat gcagcctaat gagtgagaaa taatctttcc aattaaacta
     1561 aactaactaa ttttgtttgg tcatttagtt caatcgacag ttttgtattt tcagtttttg
     1621 tctcttttgt g
//
LOCUS       KCVB01000622            1575 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29273pri ultra-conserved element locus
            uce-1290, sequence.
ACCESSION   KCVB01000622
VERSION     KCVB01000622.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29273pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1575
                     /note="ultra-conserved element locus uce-1290"
BASE COUNT          428 a          366 c          347 g          434 t
ORIGIN      
        1 aaatcaacac cccagtcagc aataatgctc tctgaggctt ggccagttta ctaaataatc
       61 gatccaaata aaagcagcaa ataaaattga ctgaccctca tatacttaat tattgcagct
      121 atttgaccaa atagcatttt tcaagatgct gtgtgtcaca ggccatcaat attcctttaa
      181 tcgctgttag ttattgccct actttgttac tgctgtggat ggccagtctc cagtggtcag
      241 tttggcccag ggcagcgagg gaatgagatg ctaaatggta gcgcaaacaa tttgattatg
      301 ctgtttaatt cccgtcatat agatctgccg ggcatgctga tgcactcatt tctgtgaggc
      361 tgtacagcag agtgctgacc tgcttagctc acaccaccgg acctaaaaga gccagttttc
      421 tctccatgac ctctggatca atagtgactg ctccctggat atgagcctgt gtcttgcagg
      481 tttcacctct taccactaac ctttcatttc ttgcatgatg gagcactgtc caacatccag
      541 tccaagttca aattttgaaa cacctagcat gctgcgtttg tggctagtcc gtaggccctc
      601 agggtttacc tagctgaacc aaggtgttgt aatacaggga gagttgaggg ttaactctcc
      661 ctattgaaac agaccacctc agccaagtga aaaatgagaa atgagcacgg caagctctca
      721 gacccagttg cccagttccc aaaattaaca tttcaattca aaaccaataa taacgcattg
      781 tgtctggcat gaatgagtgc tgctgcaaag ggaaggcgat acctcctgtt cctgtgtcaa
      841 tgaaaaagct ccttcatctt ctgctaaagg gcccacacag tctgaggtca gccagtcagc
      901 cttggttaca ctgcacaggc atgcctgggt ggatggctcg agcttcccgg tgcctttaca
      961 aaagccagga actacaaaca cacacacaca cacacacaca tgcatacata ggcatacaca
     1021 gaaacaatca tgtcatgctc tgttgatggc tctcaagttt tgccaagcag tacgtagcag
     1081 gttgaaagaa gtggacattg tgttctttga aataagttgc agtaactaat tgcaatatca
     1141 tatgaccatt tcagcctctg ctttatatta tgatttgaaa cacaataatc gtatgaggga
     1201 aaataatcat ctctccatgt gcattttact tacttttcct tctgctggtc tgatagggct
     1261 gcattcatat agtcatagca ctttatcttc aaggtattca gtctaaatgc gttatatctt
     1321 gagtgtactt actgtatgtc tctgttcatg tcaaaccctc tgatatgcta attgtgggag
     1381 atctcaggca gtcagaaagg gccccagacg aacacactgt gctgggggtt tggggcacat
     1441 tggctttgga gaatgggact gggagccagg ttctgtggct ctgccaagaa ggtgtcaggg
     1501 ccgacctctg ctacatgttc ctctccatca aagagcacat taccagtctg gaaggtgaga
     1561 gagagggaca cgggg
//
LOCUS       KCVB01000623            1543 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29316pri ultra-conserved element locus
            uce-1228, sequence.
ACCESSION   KCVB01000623
VERSION     KCVB01000623.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29316pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1543
                     /note="ultra-conserved element locus uce-1228"
BASE COUNT          503 a          334 c          275 g          431 t
ORIGIN      
        1 ccaagttcag caaacattaa gctccagcac aataaggtac atacacatgt aaaaattact
       61 cccctaacag cataaactcc cataatacac ctcagacagg cctacacctg aaagtacata
      121 ccagtacttc tcggtacaac gctgaaaatc agtttgtcac ttcagctcct tgaaaccagt
      181 gagccaaatg catcattttc acatcatgtc acgcagctga catcataatt attatacaga
      241 cagtttagtt gtaaactatt tttaccaagc tgggcactgg gaacgtcttg ccagctgagc
      301 tcctcatcct agaaatgtgt ctcataaatg acaactacat tttagctctt gttcttcctc
      361 atcttcaaat taaatttaca ctgactactt tgatatgtgc agagtgagtg tttacatccc
      421 tgtggactgt gagaaaaata aataaaccaa aactatcaca tccgcaaaca accaacaaca
      481 aatctgacat atttaccata acagccgttc tgaactttgg tctaaatgaa acacatccat
      541 aaaataagtg tttgttctgc accgtccttt gaagttggat ttgtttttgc tctttaatga
      601 tttctttctt ctgatgatga tggaagccgt gctgacacct tcttttgctt catcaagttc
      661 cactgcacaa ttacatgaat ttactttcaa taaattacca tccttgcata catcagtcaa
      721 atcatccagc tgaggcattc gcaaacaata tgcattatta atttcattgt cagtgtttat
      781 ttggttaact tgtaaccgac ttggacagcc cattgccttg atgatgcatt taaaagcatc
      841 agggtcggct ttttaaaagg aaatcagggg atgacatgct tctacacacc acttcgctcc
      901 ctgctgaggc cacagaggga aaaagactga actccagtga aagaccaatc aaaaactcaa
      961 aagatgcatc aggagctgcc aaagccaaaa caacagaatc agggagatca ttcacatcac
     1021 agcgattccc agtgagtggt tgttagcact gattgagtag atttcaattt aacaagtttc
     1081 tcctgtctag aatagttaat taaatgaata tattgtatga aagagaaaaa caacatgagc
     1141 aaacaaagag gttcagttag aaattatctc tcattaagca tgaagtcaag agttcaacaa
     1201 tttcaaacat gtcagtcatc tcttaaatca ggatgaggag agctaaaagc tcatcaggat
     1261 ttttttgttg ttgttcttcc aaacagctcg caaaaaaggc ttaactttat ttactatcag
     1321 agcctgcagt tttgtctgtg aacactgttg gtgaccatgg aaatactaac atcaggtcag
     1381 tgaggtgttg ggacacagag tataaaagac cgatgcatcc ccaaatagac atgtctgcta
     1441 atggcgggaa aaacacagct agtgaagtca aatcagagcg aagaagcacc ttctgaaagt
     1501 gccatgttgc agccttccat ttagccccga gggcacctgg agg
//
LOCUS       KCVB01000624            1725 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29406pri ultra-conserved element locus
            uce-537, sequence.
ACCESSION   KCVB01000624
VERSION     KCVB01000624.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29406pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1725
                     /note="ultra-conserved element locus uce-537"
BASE COUNT          526 a          316 c          318 g          565 t
ORIGIN      
        1 aaataaaaat aatttctaga cctaacttcc tacatttatg taattacaca gatcttctta
       61 tcttatctta tgtatatgcc tgtgttacat aacaatattg cctatactgt tcaatacttt
      121 tagcaatagt aatcaactct ggattacaga ttgagtattt ttagagacaa ccttttatca
      181 gagcattatg tgttgcctgt catgtttgat aataattgta ttttcccttt tctcttaatg
      241 aattacacat gatgaatgaa ctagttgttg aactagccag aagccacagg gtaagttcat
      301 tatacattat tccactttta aagcactgcc tgctcaccac taagagtaga tactccatgt
      361 catccttcaa gatgagtgag acacatagca gagacaggac tctgcacatc ttataactgt
      421 ccttctcggc aggtcttcaa cacacacaca cacacacaca tgctgaaagc tctcgtcttt
      481 ccatctggta aagtgggctg ttgtgattga tttgtggatt tgctccctct ttttttctct
      541 ccatttaagt gaaataatgt atggaagcaa tctggcgaca gacattttgg aaaatctatt
      601 tgaaatcatt tcagctgaag ccatggcagt ttgtcttctt aggagagagg agcaaagtat
      661 aattgtctgc agtctagtaa aacacagtat aactgccatc acacttgact gagcttatag
      721 ccagctcgtc ccatggcctc tagaagatag agcagttaat gttccctttt attttagagg
      781 atatgtcact tgtatcgcag atttatacca tgatacagca attttgtcca aacagatgga
      841 taatggtgtc accagcaaaa agccctgttt ttattgagtt tagaacatat tgtttagata
      901 ggggactgtg ttgagcagta ctgcagcgct ttctctcttt atctctctaa tcttgccagt
      961 attagatttg atcatttttg tttgaaggag gaaaatgtgg ctgtaatggc ttcctaatag
     1021 caaacacagg ggatacaaat ccagagaccc tgttctgtag tgcttccata agcaccactg
     1081 atttatagag gtctttatga cagaaaggaa aatgatctgc tgcaggtata aatcaggtga
     1141 ggagtaggaa attgaactta gatatcatct tgtcagatgc ttaatgctct tcctcctgtc
     1201 acattcgata ggaccaattt ggagaatcct gcagaggtaa aaagaagaca attaacaatg
     1261 acagtgtggt aatagatgaa gctataaaat gaacctgctg cctgcaggat gctttgtatg
     1321 atttccacat gaggtagtgg caggggtgtg ttgtctcagc cttccctttt ttccctactc
     1381 tgtttttgat ggtttcttga acattttacc cacaaaacta tgtaattaaa tgtcaaaaat
     1441 acaaaaataa agaaataatg tttagacttt ggctctgctc taaacttgaa ttattatcca
     1501 gcacatgaca ggacttgtaa atttaggtta atgttgtttt gtgtgttatt gattccgggg
     1561 acacattcta tcctaacact atataaaatt aatcaatata tcagttgtca ggcacagaaa
     1621 ttatgtacac taacattaaa agtacaaaca gccaaaataa aattctatta ctgtgttgga
     1681 agccaattca gtatatgcac gtgaatgcta agttaggcag ttttt
//
LOCUS       KCVB01000625            1593 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29448pri ultra-conserved element locus
            uce-1191, sequence.
ACCESSION   KCVB01000625
VERSION     KCVB01000625.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29448pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1593
                     /note="ultra-conserved element locus uce-1191"
BASE COUNT          415 a          341 c          336 g          501 t
ORIGIN      
        1 taatgtgggt ccattgggat caacgtgtag ttactaccaa ctggacaata attaattatg
       61 cttaaactaa ataacaaaac ttccttacaa aaatctaaat ctatatgcag tgttgtttaa
      121 ttgtaaagca cagaatattc aacatcttat gtattaagca aacaagctga aaggcagaat
      181 ggttatttga atgctttagt agttattttt ctcatctttt attaatcatt tacaccttga
      241 ctgccttaca tggaaagttc tgcaggttta gatcagaaga aaactatttt tttgcttaaa
      301 ttttgatcgt gactggaaca aggaaccatt gtctttctgt gctcacacta caagctaacc
      361 tgcatcaaat gtccaccaaa aagttattta tgtctgtcca acttaatcca gtatcctttt
      421 gtcctgcacc tcagtaaact aagggttaat attgtctgga gtccagctga ggattaggaa
      481 atggaggttt aactgcctgt gctaatggtg ggtgtttatc tcaatacttt gttttgtcac
      541 tcccctactc gctctcattt ccgaaagctg tccctggagc ctccgctctt gtaatggctt
      601 agctttctga gcgccacagt atggagggaa aggaataaca agattctcat ttgggctcca
      661 tacacactgc ccacagatac atgtataatt ggccctgttt tcactccact ctgttgttaa
      721 atgcactagc tattagggta gtggtattgg tgctgatttc atgtgtttgt ttattgttgg
      781 aaggtgtgtt gtgctccttt gtctgagcac gtgcgtgtgc atgtaaaagt gtgcagaagt
      841 gtgtgtgtgc acgagcgttt taatgtgtgt gtgtcagata gataaagagt gtgctctatc
      901 tctctttctc tccctgcccg ttctcttctt tctgagtttg gtcgtggttc ccctggggcc
      961 cctgtcgcag taatggcctg ctcctgattg ctctgataca ggttaggaat attggcttta
     1021 atgatgtgta aaatgagcaa gcagtgcctg tgacatttac agatggcact gccttggcct
     1081 gttcttccca actgctcgtt aattcacggg aacggcgagc acttctttaa gtgactgcct
     1141 gatgcatgca tacgacatgg cactacacgt cctgcacacc aaaacaatag ctcaccttgt
     1201 gtacctctgc accttgtcat catttcagcc aagcattcac acaaatggat aaagaaagag
     1261 tccttaaggc taagaactgt ttgctaagtc ctttctaagg gttcgtcact ccagggtcca
     1321 gaggtgtttg agaggctcag acagaataga tgctgcctgc cgtacagaca gcacagctgg
     1381 gctcagcagc aaaccctcga ggtgctaatc tggtccacag gtcctcaacg gtgcatctgc
     1441 tgcacataca cagcaatagt ggagtcacca tcatgaggtc gacctctttc ccttatattt
     1501 caggttccac tttgagacct tctcgaggtt ttataatgtg acaaacagca cctgaagaag
     1561 tttaaaaaaa ggaatacttt attttaatga ata
//
LOCUS       KCVB01000626            1581 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29489pri ultra-conserved element locus
            uce-1215, sequence.
ACCESSION   KCVB01000626
VERSION     KCVB01000626.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29489pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1581
                     /note="ultra-conserved element locus uce-1215"
BASE COUNT          490 a          303 c          310 g          478 t
ORIGIN      
        1 ttcagacaga ctcaatattt tcctcaatgt taacatgatc ttcttcacat aacaattacc
       61 agtagctttg tctgggaaag tgatgtgaca atgcaaccac tggcgtttat gagaactgat
      121 gtcagtttgc attcggagca attaaaatga gtaaaattac tttttatgag agaacacaag
      181 caatgaggat tattttcttc cctggtgaca tcctgtaatc agctaaacat tttcattttg
      241 taaatatttc cattttttta gatggaggaa tacatctcca tgcgcagtac gacttcttaa
      301 acacgaggag cgatccctaa aacctttttg cgataaaaca cacaagtctc ttgaggatta
      361 catgcaaaaa ctgtctcata atgttcattc atacggtgag actacagtga gcagcgagct
      421 attttgttat cacgtcaaat tagtggatcc taatcttcag ctttattcct aaatttaaat
      481 ctgcgattct aaattcgcac agatcccttt gctgagggct ttggctgcaa aaatctgtca
      541 ccgatgctgg tgtgctgtac attaagggag aaaacgctct taaaaaggtg cttttctgac
      601 attaaaatga cttgtgtgca cagcgcatgt cgatccgtgc agttcgaggc atatatttat
      661 gaataggaac ctttaattag ggcttgtgtt tcctccctag tcacattgag ggatggtcct
      721 cgccgacatt cattaatgat aatcaccaag agcgatggca tagctacagg ccaagtctca
      781 ggggacccga cacttgaaaa gaagcattaa attacacaga gggactcttt aattgcaaaa
      841 aggaaaatat atagccatca ttgttaaata tgatctttgt aagcctttac tggttcaagg
      901 atttgtctaa gggtttctct ttcaaaggaa tctcgagcta catttctgcg ataatacacc
      961 acagggccct tttttaaaaa cacttcattt tacccctcct ggttttcttt attgcaagcc
     1021 aagcaaaaac tgtggatata tatcagtttt atacatttgc attttgttgt aaattcagat
     1081 tcgacatgga attcaaattg caagccgcat atcaaactgg ttacattaac agcttcaaag
     1141 aaagaaaata acagcgagga agatataagg gaccaaattt caggcgagtt tagaccttaa
     1201 acacaacact gagtctcctc atcctcgaca tgtcttaaac atgcagttgt cagtccagaa
     1261 ttgtgcatca agattatgtc agattggaga agaccgacat gttactgatg cagaaatcca
     1321 tctttcacac tttctcaaaa aaatgctaaa atgatagcta acaaccagca ttcattgtac
     1381 tgctcagggt tgtgaactta ggtgtggggg gtaagttcac aacttgcaag gaggattatg
     1441 tggaaggagc cgctgccagt atataggtat acaaacatta ggttacggag gtcatgaaaa
     1501 tatgtttaat tagaaaagat tcaggattct taagggatca atcaggggat gttttgctct
     1561 ctggcggcca tttttaacct c
//
LOCUS       KCVB01000627            1753 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29530pri ultra-conserved element locus
            uce-1250, sequence.
ACCESSION   KCVB01000627
VERSION     KCVB01000627.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29530pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1753
                     /note="ultra-conserved element locus uce-1250"
BASE COUNT          433 a          448 c          382 g          490 t
ORIGIN      
        1 tttaaaaaaa aaaaatgggt aaactggcaa gaaaaacaag gataacttga agacaggcag
       61 ggagtgagga tagattgcag acaatttatc cagagccaaa ggtgctaagg gtagaggagt
      121 tgtacttctg tctctctgag ctgcagggat cctgtcatac agtgcttata tctgtgaaag
      181 ctgttattga tgtaattgta ttgaagttct agcgaccatc tgttctcctt tgggagggcc
      241 tggctcctct cgtcggcagt gaagtggtta tgtgcagcat atcactgctg ccgacgtgct
      301 tgcagagaaa aaacttcctt ttcagatttt cccaacggct gctaataaaa tccagatgct
      361 agactggagc tgtttctgcc tcgctccttc accgctccct cctcctcctt ctctcctccc
      421 tctccatctt ctgtccttct acatctcttc ttttagtccc ccctgcctcc ttctctcctg
      481 tccttcacca ttgctaatca tttatttgac tcttttcatc tacctctccc ctttttcgct
      541 ccctccctcc tcccccgtga tccatccatc ctcgcctcct tctttctctc cagccactaa
      601 cccagagtca ttcctaaata aaggaaagta ttcgtgacat tcggccgagg cggctgtgcc
      661 aagaacaact ccctggctag ccagtcacat ctggagtgtg ttaggactgc aatgggcacc
      721 ggtaccaaca ctggctatcc caccacatgt acacacacag agaatggtca caaataccag
      781 acctcaggaa tcatatacaa actagactgc atacattgtc acagtgacca gcttgttttc
      841 atctcacatt aaaagctgca attcttgttg ctcgccagac agcccagcag ctgtttgcac
      901 gaattctctg ttgtgcctgt ttctgtgttg aatacacttc cacagcagca acagagaaat
      961 acagagttta tatgcataat tgtgcaagta catgactact tgcatgtatt ctgtgtatgc
     1021 ctgtgaaccg cattagagtg catgcatgtg tattgtgcat ctgcgtgtat ctgtatctct
     1081 ctcaatgtgt gtgttgtgtg tgtgggtgac tccagggtct ctgtgtgaga gcagttgaac
     1141 acctgaacat caataactgt catgtgggcc acctgggacc ccacagccag cttgcaccat
     1201 gggcacacag ccatcaccag cacagctgcc acagagagag agagagagcg agagagagag
     1261 ggtgggggga gggagatatg ggggaggaag taggagacaa gaaagagaca cacacatggc
     1321 tatatgagga ggtgactgtg catacacatc tgggaatggg tcagagagac agaaaggcag
     1381 acaggcaaga gcagagagta ggatttacat gaagcttctg ctctacagga ttactctcct
     1441 ttgttccctc tcatctacat cttcttttcc tcgtcctgtc agccgtgcgc ctaattttgc
     1501 cagctgggag ctcagcctca ggctgccaca tttcaacatc agatcagact ttctttcgtc
     1561 attctgcttt cagatgagct tagttatcag gctccatcag atgtgacatc attcctttta
     1621 atatccagta cactgttgat atagtgctac gaaagagttc tgcatgagcc tacaaacttt
     1681 gcctgttttt tttttagatc taacctgatt aaccccgact ggcattgcca catttgggac
     1741 ctgagcccaa ctt
//
LOCUS       KCVB01000628            1414 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29571pri ultra-conserved element locus
            uce-262, sequence.
ACCESSION   KCVB01000628
VERSION     KCVB01000628.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29571pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1414
                     /note="ultra-conserved element locus uce-262"
BASE COUNT          382 a          282 c          359 g          391 t
ORIGIN      
        1 aatatttagt tattatgtag attaatttca tttcacattt tcatgtctta taagttaaat
       61 caaattcttg aaagatgttt tgctctctga ctttgtgtga atgtacatgc tttgttcatg
      121 cattcatgtg tgtgaatgtg tgtgtgtgca gaggaagtgg ggttaaactg caaagtgaaa
      181 ctgtgcgact gaatctaatg gaactaaact actactgttt gtttgaaaac tgaaagataa
      241 agcaacatac tcagggaact agttggccag atatgaagct acatttttaa gaattctttc
      301 tcattcgtgc ccttgagcgc actgacaggc aggcagagag agagagagag ccacgaaagg
      361 aaaaggaaga gagggcgcct cctatcacga atcaagccag tcagattaca gcgactttaa
      421 ggaagatttt tcaaatgttt gcagatgttg gcatattttg agctgttttt ttatgtggtg
      481 aagggaagag cctgtgcggt tgaatgttgt aaaaactcag ctttgccttc atcaggcaag
      541 gccaaatcac acgaggcgag ggagctaagt gattgttagt aggcaagccg cgtgagcctt
      601 tgatctctcc taaataaaag ggaaagcctc acgtcgcgca gtgctgattt cagtaccgta
      661 acgtcacctc tttgatgcat ggaaatattg tgacattgtg tgggatgagt tcttttgtca
      721 tgctttcaga aggctgcctt ttcaaagaga agagtgaaaa agaggaacag cttgtaggga
      781 aggcttagtt tcacaaggag ctgatatgaa ggtgtatcta gaggcctctg agaggcgtca
      841 tgagactggt gtgtgtgttg catcagaaaa acactgtaca attgtttaca catacagtag
      901 catcctacat agcagtctca tactgtatat tgtatattac atttaacata gatttgctac
      961 tatcttggcc aggccctaat tcttcagaaa caaaagcatg ggtgttacag agagtgaggg
     1021 aatgagtcag tttttgaaac aagacaggga taaactttcg tttataactt acagaacatc
     1081 ctcagtggtt cctggtcttc ctccttgtca gcggaagatg cggtggtggc gtcctccgtg
     1141 acctcggcct cgggctccac ctgcccccag tagttcctgc tgctggggct gaggctttgg
     1201 ggccgcgtgt agcgagcagg gctgccaggc tcctccgtcc cctcgctggg tgtcaggctt
     1261 gtcttgtcca ggttctcctg gcagtcctgg ggctccaggc tccacaggcc aacttcatcc
     1321 tccccctcag atcccagtga actcacttgc tcagcatcca ctgagggaag aagagaaaag
     1381 gcaagacaaa atttgagagg gagagaaaag acac
//
LOCUS       KCVB01000629            1239 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29616pri ultra-conserved element locus
            uce-227, sequence.
ACCESSION   KCVB01000629
VERSION     KCVB01000629.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29616pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1239
                     /note="ultra-conserved element locus uce-227"
BASE COUNT          318 a          226 c          307 g          388 t
ORIGIN      
        1 aaagaggata taaagaagga gagctgtttt cttttcagtg tctcccctcg gctcagtctg
       61 gggtaagccc actaatcctg cttggtgtga tgactcacaa cgggtgtggc caatgtgttc
      121 ccaaagatat cttttccaca gtttgaatat caataggaat gtttaggttg gctctgtgaa
      181 tcatgttgtg tggcatgtaa tagtttctaa tagctgctga acacctcttc ttatgtttat
      241 atgtatatgt ttgagccata gaaagcaaca aatcagaaaa aacattcatt tctcatattt
      301 gtgcaatttt ttttagatgt atgatgtagt tgtatgagcg tgtgtgtttt ctgccatatg
      361 tgtgtgtttg tgtatgtgtg agagggagag aattggacca gtgtcccacc taattgttat
      421 tgcgtctgtg tttggcctgg agacagtcga taggaatcag cagtgagcct ggtgctggtg
      481 gtaggggggt tatggagggc tgtgtgtctc ccacttccca gtgtcttagt tgtaatgcat
      541 ccctccacta acagggatca aggtattagg gattcaggtt atttgtattg attttatgtg
      601 ctgtcatgcc gggctcgagg acgccctcat atttgtggaa attgcaaatt ccacctcgga
      661 aatgtcacca gcgtttaatc tcttcctgag tggtcaatac aggaccctca ctctggtccg
      721 accacgttta ttaaacagag accaagcgtt cccacggttg ccatgacacc acgcagcctg
      781 gaatgggatt ggtccaggtt agatggtaac atgggagtga agaagtcgaa cttcaacatt
      841 aataactttt gatggatctg tgtcactggt tatgagattg gacggggatg aagagagttg
      901 actgtggatt agattcagca cagggagaag tagtcatagt tgggtttggg agtcacactg
      961 agaatataaa cagaggtcat tctgaagggc tgcaaacttg ctgtactgta agtctgcgtg
     1021 attaacaata atgtgcattc aaaaggcttt ggtgccttta aaatatgtat taaaagaagc
     1081 attcatgtcc tgacatcact gcaaactgac tactagagtg tacaaactaa ggtcagtgtc
     1141 attattctac gaggactctt caaggtccgt taaagcaaac tgctttgttg cgtctgccaa
     1201 catttgcatg ctattatcat ctttgcttta tccgtgtca
//
LOCUS       KCVB01000630            1437 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29657pri ultra-conserved element locus
            uce-1114, sequence.
ACCESSION   KCVB01000630
VERSION     KCVB01000630.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29657pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1437
                     /note="ultra-conserved element locus uce-1114"
BASE COUNT          299 a          420 c          268 g          450 t
ORIGIN      
        1 gtgtctccac ctccctttct gtctctcagg ttgccccgtc cctgtctaag cggttgaaca
       61 ggtagatcgt gatctttcac acagcagaag gatcttattg gtcaaaattg acggctgcgt
      121 tagttgaaaa ctaaccagca caggcggggg cctgccgtac tttcatctcc taccaagcac
      181 ttcaattaag gctcctttca tccaagcctc attcttcact tcagagcggt gctggaggga
      241 aggccaggct gaggcccaga gattgaatct ctgcgcgtct gtgatgatct gtctttctgc
      301 cagtttcagg cttccaccct tgctcactgt tgtcctctaa aaaccactct gtgattatct
      361 gtctttctat ccctccgtcc ctagtcgatg tactggctgc agcctccttt ttgctattcc
      421 tttcattctc acgatgacct tatccctctc cctgtctttt aatcacccga tgcttctttc
      481 ttattcctgc tctttctggt cctgacccac aatctgctct catccatcca cctttccatc
      541 ctcacctcta ttcttgtctt cactttttat catcccctgc ccactctgaa acttctctct
      601 ctctgcctcc atttcattct ctctgtctct cattctttcc ctcccgtctc ccactctcgc
      661 tctctctccg ggtagtgctc agctgtagag cacagcggac tctaatggca gatcattatc
      721 atgctaatta gagcactttg tagctgccgc ccgtcattct ctcctccccg cagcccccca
      781 cgctatcgtt aaaaaggcca ccccctcctt tcccctctcc accaactcgc ccacccccca
      841 cctcgtctct ccatcattaa cgggacgtgc gctggtgtaa ttggacgctg tttattccgg
      901 agagcgggtt attaaatcac accgaggtga cgctcgtgca ggtctcccct gaaacgcttt
      961 catcattgtc ccatggcatt attgaacctg cgtggacgcg cacaaacaca tgcactcaca
     1021 cacatgtttc aaagaggata ttcttcgtgt atatcgatgt tccctacaag atgtgaagga
     1081 acatgatgtg gagagcagac tttccctgat tcctgctgtg aaacaaacgc ctaccgtttc
     1141 agattcccct gccaataaaa gtttaatttg ataatgatca gtgaaatttt tttgacccgc
     1201 aggcttggaa gacaagtgtg gaccaagtta aattaggcca aggacctaat atttgccttt
     1261 taagatgctg ctaaagttgt agtttttagc acacagcaag gactttttag gcacagtggt
     1321 ggacatttat tccccatcca ttttcctttg aaaccatgtg tgtctgtatt tgtgggtcat
     1381 agacagattc agtctctgtt ttgatagtca gttgaagttt ttgctctgaa tcatgat
//
LOCUS       KCVB01000631            1095 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29692pri ultra-conserved element locus
            uce-1151, sequence.
ACCESSION   KCVB01000631
VERSION     KCVB01000631.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29692pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1095
                     /note="ultra-conserved element locus uce-1151"
BASE COUNT          269 a          313 c          223 g          290 t
ORIGIN      
        1 tccccccccc cccccccatt tctgtctgtg cgaggcaggc tggctctcgg tgttgacctg
       61 agaaggactg acattcagag gccggtcccc tgcctgcagc actgacattt ctgccagact
      121 ttgatggtgc ctttgaacag agatgtgtgg agggaagagc tgcctgtctc tccgccttcc
      181 agcagctcta gctctgcttc tccaccactc agactgagat gtactgtagc gggctgctct
      241 cccatcacac atgcaggaaa tatgcgagtg catatacatg aatagcgttg aacgtacata
      301 cacacacctg aactcattta gttagcctca tttactcagg tgcttattta tcacgtgcaa
      361 acattgatgt tctcacacac atctgagcag atgaatctat tcacacatat gtgtgaaaaa
      421 cacacatgca cacacacaca catgcacaat cacacacctc ctgctgggac gccttcaaag
      481 accctcattg cccccctccg ataaaactgg cagagagaca gagggtgttg gaggggacca
      541 ggttatctct cccacctctc ccctaactgc ccaaccccct ccaccttact ccccctgctg
      601 tggggccttc aaagacaccc atgtgactcc agcaaccctg tagctccact cctcctcctc
      661 ctcctcttct tctttctcag tgccttccta tcttctctaa cctctcctct gcacatcaca
      721 ctcccttggt taccagtttc tcacaaggat acaaaacaca gaggaggaaa acataaataa
      781 ttaaaaaaaa tagagacagg attgcttgac ggggggttgc tcctctgaca attagtacaa
      841 aatgatgtaa aaatggcact ctgttccgtt attcagtaaa tgccacttct gtgccaaact
      901 gcctgctttg gcagataccc cccctcctct gctgtcacta aggggaatgg gatttttctt
      961 tttcatgtgt tggaggtagg gaccaaagca tgtggtgctc tgtgagtcta attaaaatga
     1021 cttggctatt ttgccatgca atggtgtctc agagaggtgg atgagaattg aaacacttaa
     1081 ttttgtttcg cttgg
//
LOCUS       KCVB01000632            1404 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29775pri ultra-conserved element locus
            uce-163, sequence.
ACCESSION   KCVB01000632
VERSION     KCVB01000632.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29775pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1404
                     /note="ultra-conserved element locus uce-163"
BASE COUNT          463 a          263 c          242 g          436 t
ORIGIN      
        1 cccagcctat tgcactgatc acatttgaat tatcaaaatc aataatcaga taaccttctg
       61 tattttacct ttagtaaaaa tggctgcatg tgtctggatt aagataaaac tggggataca
      121 gagtctgtat cttttgttgt ttttaaaaaa aatattatta gttggccatg ctaaatcata
      181 ctaaacattc acaagcagtc cataaagcaa agcctttaca acagcaaaag agacgcatag
      241 cacctaaagc taatgcacac ttcgtctgcc ttgattccat gtcaccaaag catcccatca
      301 aagcctgcct ccttcctgtt gtaaggtagg aaaaaaaaaa tcaatgctgc aatcttttct
      361 ttttgcctcc tccaatccgt aattgaaatg gaaaatcaaa tgaacggcaa gcagataatt
      421 gttttatcaa tattccctgc ctgccctaca agggtcagtg cagaatgatc atgagtcggg
      481 gatatgacga ggacaaaatg tgaaattgaa cagctaggag gcaataaatc agccttgaca
      541 actaagagtg accagcatgt agctgcagca ggctgctctc ccttcagaat ttgtcctaaa
      601 gctgtctact ctaaatgggg aatattcatc tcattattgt tgcacttaat attgctatca
      661 aatattattc ttagagtcca gacggtaagt caaggtcacc catgctatta ttgttgtcat
      721 tgttagtgtg tgttattgag agtataatag agggcagagc catatggtct gctgagctta
      781 tgaagagaca aagaataaat ttagtcattt gttagagttg aggaaagaag tgttttgctc
      841 tttgacatat gtcattttta ctgctgcatg taaatatcta tgaaattttg gaaatcaaca
      901 aaatgtacga caacactttg caccaacagc tacattaaca ccatcatcaa caattctgcc
      961 attggttggt tgatgcactt gtcagttttt ttactgtcaa actgttgatg cttgcggcac
     1021 taaagtgtgt tagctccgga atataaatta gtaactgtta acaaataact gacattaata
     1081 ctttgatgat caaccaatgt ttgaatgagc tgatgtttgt aggttaaaat tctacaaata
     1141 ttggttgaag acagagtgct aagtgctaag acactatcca ttaacgccaa ttagttcctt
     1201 aacttaccag aaaatctcag aaaactgaaa actccccatc acaattcttt atttggttca
     1261 accttcactt taaaatttac ttcagaaata gcagtaaata aacattaaat aaaaggttta
     1321 aaatacacta taatataagt tcttattaat atatttgttt acaaccataa ctcctcctgt
     1381 gggcacctgt aagtcactgc tggt
//
LOCUS       KCVB01000633            1002 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29811pri ultra-conserved element locus
            uce-965, sequence.
ACCESSION   KCVB01000633
VERSION     KCVB01000633.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29811pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1002
                     /note="ultra-conserved element locus uce-965"
BASE COUNT          339 a          217 c          214 g          232 t
ORIGIN      
        1 cacacacaca cacacacaca cacagaggaa gaaacaggga gaccattgaa attcatctca
       61 cagcctgaat ttacttaact gcgggtctgt gcgcggtgat aaacggagtg atgatccggc
      121 aacacgtctt tatccatgct ttgctttaat taacggcggc ttgtttgttg aaaagatgat
      181 taatatgcca atgaaaattg cataattgaa taccacaaca cccgcacaat cagggaggaa
      241 catgccattt tttattaact ttttattatc attattataa ctattatttg gtattttaat
      301 tcacttgaca agcgggcccc accccccacc acccctacac agaaaaaaaa gaaggaggtg
      361 gaattggcgt gaccagaggg ccaagctggg cccagctgtt catcaccaat gagaggcctt
      421 cacaggcgag ggcactgttc tgatatcaat acctatcagt gtgaaggcgt gtgagagaga
      481 aagagtaggg ggttgggggt ttttagggga gatatggctt ggatgggact aagtgcattc
      541 ctatggccaa ataataacgt gaggacacca aacccaatga aagtaaaaca gtcatgcata
      601 caaaactaag cactatcagg agcacttgca tccagatttt gcaaagagaa gcctcgcaaa
      661 ttcatgcatg aaaagaacta caagccaaag ttttagtagt tttatttaaa aaaaggccaa
      721 aagaaatgaa aatttacaca tgcaagtaag tttcagttga gcaatccaga gtttacccag
      781 cattatatcc cccagcacag cttgcacgag tgtctccatg ttaatgctaa atacacacat
      841 caaatattca accaattaca atgatacctc ttagaaaaac gtgtgcagct acagcgcctg
      901 ggagggtaag cattgcagaa caaacatatt caaagagcta aagaaagacg cgatgggtgt
      961 caagtccccg agggcagaaa caccaccacc agccgcgctt ta
//
LOCUS       KCVB01000634            1462 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29886pri ultra-conserved element locus
            uce-753, sequence.
ACCESSION   KCVB01000634
VERSION     KCVB01000634.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29886pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1462
                     /note="ultra-conserved element locus uce-753"
BASE COUNT          424 a          289 c          261 g          488 t
ORIGIN      
        1 cctacatgac aaagtgaagg gttacagtgt cataatttta actggctgac atcaattgtg
       61 tgctcctaca tttgaatttt gaaagtttga aagtgttaac caagtaaact tttctctaat
      121 aatctgagct ctattttatg cttttaacaa gacagaatct tcttttttcg attgatgttc
      181 tgtaagcaga gtcttttact gttctacaac catcagagct gctctgtctt tccctcttgg
      241 acctacccta gactgcagta tgtgtttttc acactgacgc taattcaaaa agacattgtc
      301 catttatagg gcagtctccg tggaacaagg aacgagctgt tctccttcca aactggctta
      361 ttaagtgcca ttagtgtttt tgcatcaatc tttctcaatt atcatctatg tttttttttc
      421 ctataaaaat tgtgggagaa gcttcaaatc catttaagct tttaaagatg tggtctaaga
      481 taggtcctat cacttttttt ctgtggagat aaactgtaat tactttaatc tgctttaaca
      541 ttcctcattc ctctttttcc tgaccctgtc tagttctttc cattcttctc tctctttaca
      601 gacgtttatc tttcagtcta ccaggcagga caggtagaca caggtactgc caaatttaca
      661 accaagtact gtaactttaa ctgaagttta cttccccctc cccccccccc gagtctctcc
      721 atatggcctt tcatgtaata gcaaactgac gtcactaaca tacagtagga atctttcatg
      781 gcagacccaa aaatgttcct attgctcttt aaaacttaca gtagttcttg acactttggt
      841 gaatgtgaag gggggctgga taatagaaac agctgacagt ataatccagt atattacaat
      901 agaccaaaaa agactatctt tgcaaagttt gtaaagatct gcggttgaga ctgtgtcata
      961 taggtggttg tccagcattt ttgaagctga agtgtgtggt agtggactat acaaattatc
     1021 cagtgtacct attatattgt ccaaccactg tagtttaaag aaatgtgaat ctaacaggct
     1081 acagaggaca cagaactcta tggaaacacg ccatttttaa gtcatgttga gctctttaac
     1141 acagctttag aacacattgt tatcaaatat tttacatgat ttgtctcttt gggtaactgt
     1201 gacaccatca aaacttatct gacataaaat gttgcagtgc atcaaaggaa gagttggata
     1261 ttttaggaaa tgtgctcatt ccttgcttga gatgaaagga tccaagccat tttatggtag
     1321 atatgtagct atagctagca gctggttagc ttaacttagc ataagaatgg aaactgaaga
     1381 aaacagccag ccgggctttg tctagaggaa acaatctccg taagaggacc tcttaagctc
     1441 acttattaaa agtttatatc tt
//
LOCUS       KCVB01000635            1154 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 29952pri ultra-conserved element locus
            uce-878, sequence.
ACCESSION   KCVB01000635
VERSION     KCVB01000635.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="29952pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1154
                     /note="ultra-conserved element locus uce-878"
BASE COUNT          353 a          200 c          229 g          372 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgagagag agagagatag agagggagag agagagaggg
       61 ggaggatggg ggaagggcct cagtctgcaa gatcagcaca agtggaattg cattgattca
      121 gttaactgca gagttggagc gtgatgtgga attctgttta ttaagggtgg agtttacacg
      181 ataggctcca ttttgatctt attttctaca tcgcattcat aatcagtccc tgctctctca
      241 ctttcagcag taaaatatta gatcatcttt acacatatag cttaacccca cctggagttt
      301 cacatgtgca ataaaaagag ccggactgat ttaatgtctt aatttaaatg gtaacataac
      361 gtatttccca agtgtctttt ggaccaaaaa aaacataact agaaatgctc gcacagctca
      421 gatgggataa tacgggcaga tacacactgt aaacatcatc acgatctgta ttaatctaca
      481 aaatcggatg taaacaactg acatcatgta ctgtaagtca cagagaatgt gctgcatgtc
      541 ccaagtaaat actgactcat gttttatatt caaattgaag ccactggccc agaaacaaca
      601 tctgctgaat cttaaaaatc tgctaaataa atctcaaacg ctgtttgaat tatagagagt
      661 aaaagaaaaa aaaatactgt agctttttaa ttggctgttt cttatgaatg aggagtgttt
      721 ggtggtttgg aaaatacaca ggcaatactg aggatgtgat gcttttattt tactaaaagt
      781 acaatataga aggtaaaatc ttaacctgac tgatgttctc atattatata aaaaaactgg
      841 ctgatataaa aaaaacccta ttgctggtgt tttatatatt taaaagccat ttatgtgcag
      901 tctgccattt ttttgtcatt cagggaggat ggatgtatct taaattaatg aggctttctc
      961 ttaaacatgc atttaaaacg agcatgttgc agatgtatat tttcttttct cctttacctc
     1021 tctccccctc tctctagctg tagctgtagg gaatttgttt ctaagggttt atcatgactg
     1081 cctcatattt ccccccctag gttgtcattt tgctgatcca gacgcttttt catatttcac
     1141 tattcttatc atcc
//
LOCUS       KCVB01000636            1122 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30035pri ultra-conserved element locus
            uce-885, sequence.
ACCESSION   KCVB01000636
VERSION     KCVB01000636.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30035pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1122
                     /note="ultra-conserved element locus uce-885"
BASE COUNT          334 a          209 c          244 g          335 t
ORIGIN      
        1 aaataattat gggattttta tgagttactc tccttttttc caactttagc atgttgtagg
       61 attttgtgtg tgggtgtgca agttcacacc tgagataata gctggatgag ataaggcaat
      121 caactcctct taacaaaaga aatcaggtaa gtacctctct cgcagaagaa cttaaagtag
      181 ctctgattcc ggtgaagagg tgtctgtccc tccagtgtgc atattactgc atctcatctt
      241 gcttattgct gcctagagtg ctatgacaac agaagtgaga atagatttaa tctctccagc
      301 cctgctaata atactatgtt actgccagag caccgaaaca ggcacaaagg gctaatcatc
      361 tctagcacta agcgttttct tacacatatg tacacatttc cccggcagtt ctcagaactg
      421 tcaggagtaa agagataaag aatgggcaga aggaaaaaaa agtcagccgt ggtgtgagaa
      481 gtgtctgcca ccacggcgtc attaacaccc agtggtgctg gagtaaggga gggtgagcag
      541 acggggcctc cgagcactag gagcgaaatt gcaaacccaa ggttcctgat acaacctgtt
      601 attatttttg acacaagcca tttacaatag ctatgaaggt ggcaatggaa aatgtaatat
      661 tgattgtatg ccctcaaggc tttgctcccc tgacttctaa acgtcacaca tctcacatat
      721 gttggatgtg atgatatgtt tctctttttt tttattttgt tttgttttcc ccactttcac
      781 aaatcctatt ttaggggttt ttgctgatag ttttatttaa tgaggtaatt acattggtag
      841 attgcgaggg taaggtgatt ttttggggct gggagaataa tagaaagctg ggtaactaca
      901 gaaacgcaat atgatatcag tgcgatgtga cagttgttaa tatacccagt ggcaatctgt
      961 atttcatctc tttttcatga gggtgcttac tgaattactt ccaaatgacg tttatgaaac
     1021 attaagcaac tggtgcacca tgggacagca gtggctcatt tcagaattat taagatacac
     1081 ttaaatatac agtgcaccaa agacacagtg agaaaaaaaa aa
//
LOCUS       KCVB01000637            1509 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30120pri ultra-conserved element locus
            uce-1308, sequence.
ACCESSION   KCVB01000637
VERSION     KCVB01000637.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30120pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1509
                     /note="ultra-conserved element locus uce-1308"
BASE COUNT          527 a          284 c          304 g          394 t
ORIGIN      
        1 ggaggaagaa gttatacgag ggttgcttgt taggacatgc ttgctttgga atgcagggac
       61 caagggcccc ctgaatggag agagagagag aaagaaagag ggagtgagaa aaaggttaga
      121 tcaggaacac atctactgtc aacgaatttg aggctaatga aggatgaagc ttcacctctt
      181 tctttggcag gggcttgcag ctccattttt ttttggtgag ctgcaagggt gctatctggc
      241 ctgtcacctc gaatttctct ccccagccaa accggaggaa cggaaagctc ggtgcttcct
      301 cctccactga aggctgggtt ttctatgcca accagagcag ctctctcctc tctccctgac
      361 agaataagta gaaacataaa taaaaataca aacactgaca tgaaggagca atacaacagt
      421 taggtcaaag cagcttgtaa aagaaacaaa acaacacaca caaaaaggag catatctaat
      481 gattgttttc tattggtgat ttctctgctc caaaatgttc aagcgcatcc tcctcgactg
      541 acactaaaac acaattaggt taaaattaaa tcagaatctt gcaaagaagg agaaaaaaaa
      601 agagaagaag aagaaatgat taaaataatt acaaattgcg ccctgacaag caagagcaaa
      661 atttagccca aagccatttg tctgtaatta gttaattaat ccttacacaa attatgagca
      721 ataactcatc aagcaggact cacccatcag aaattcagcc gacttggatt tcttctgctt
      781 aggttgctgc agagccttct gctgtaactt ctggagggaa attgttaaat gaataaatta
      841 gctgcagaat gaagatcatc atttatgatc cgcaactaac gcagtatttt aaatatggga
      901 cgacactaaa atgtcattta atttacatcg ccgctaaagg ggtcaagcac taattgaaca
      961 acaggagtgg aaaagaattt aatcataaat agataagaaa agttgacatg aatgtgtaat
     1021 gaaggtcaca ttaaaatact tcctccttgt ttctaaaatg aatgggcaac aacactctct
     1081 cctggaaaat tatgcatttt tacacaggct gcatgagagt acacagactt ggtgtgcaaa
     1141 tgagtatgga aatcaaggca cacttctcat tacttccatt cacatgaact caacctacaa
     1201 aacctcaaaa gaagagagaa aaatagtctt taaataacac ctcaccattt cccttgtatt
     1261 tttgcaaaag ttggagaatt ttcactatag tacaatacag gatttattca taaagcagtg
     1321 attaattcta tctatctatc catctatcta tctatctaaa cacatgataa gtcaacattt
     1381 gactatagtt ttgtaggtgg tctgcatggc atggcatggc gtggaatgga aagaaagagg
     1441 agggatgaat gaaagcaagt atgggttcct ttgaaatcaa gagtatgtgg aacaaactct
     1501 gcagtccca
//
LOCUS       KCVB01000638            1615 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30161pri ultra-conserved element locus
            uce-1172, sequence.
ACCESSION   KCVB01000638
VERSION     KCVB01000638.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30161pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1615
                     /note="ultra-conserved element locus uce-1172"
BASE COUNT          379 a          407 c          308 g          521 t
ORIGIN      
        1 ataatattaa gatgccatat tgggttcttg gaaattgtaa tgggctactt ttcacatttc
       61 atagtctaaa ttgttaatta ataaaacgta gttgcggctc tagagggtct ggtttgcatg
      121 gtccgccagt cttatttctc tgttttgaac tggttgagca taaacagtag actgtcctcc
      181 ctctctcata tcctgatgta acttgtacct cattttccta agaagcattc tatgattcag
      241 cccatccaca cacatttacc cacacaccaa cacacacaaa ctacacacac gcctccccta
      301 cctctctaca aaacccaaca atccagcaaa atccgtacaa cttccttctg ttctcaaacc
      361 aaaagcctta aagccaacag atgctttctc gttatcattg ccagttatca ctcttagctc
      421 cctctgataa gactttactt tctgacccag atgttaagga gttcaattta ccagatgcac
      481 cagaagagcg gacagggagg tggagggatg agtgagcatc gaaaatacca gcaggcttga
      541 gagatgagac gtaatatgcc attttttatt tttttttttg gcctggtgtt gtttctgaag
      601 cggcgttagt gcagtaagga gctcagcgtc taaagaggct ttggtatagg gggaaagatg
      661 ggccatatta cagcggggcc agaagcctgc ttgattaatg ggccaggata atcacctcat
      721 caaatcactc tcacttatta aatcttgttt aatatcgtaa tgagggccct cctgagctgc
      781 tttggtaaat tccccgtctc tccatagctc tcgctcttcc tcttctgcct ttccatttta
      841 acaggaagac taatgatgcc aagttcctct ctttcctcat cttcctctct ctgtcttttc
      901 ctctcgctgt ctcttgcctg ttggcgtttt tccttacctt tgtcttctct tttctaaacc
      961 gttctggaat ctgtgtctta gattttagac cacacagtgg ctcaactgta tacataattt
     1021 caaagggtgg aattactaca tacacagcct cagtctattc atttaatccc ccttatttat
     1081 gatggatgat gtgctagatt gtatcatcat aaatcactgt gtgtgtgggt caggacctgc
     1141 ttgaatagtg ataccacttc ccaaggcttt ggtacactgg agtttatgga gacacttcag
     1201 gtgaggtaat atctcccctg tctccttcta tgtgatttgt gatctctcta tggagtatca
     1261 cgacataccc ggtgttactt ccctctatgc atgtgtgtgt gtgtgtttga ggtttctgag
     1321 atgggcttgc atctccttca cttgacctcc aggcgactac acggtgtgcc tcagaagaaa
     1381 acaacatgcc actgtcttat ttatggtctt ccatgtgtca ctgggtacca tcttggtagc
     1441 gcagccgtga ctgctcgctt cttccaagga agggaaaaat atttctcctt tccccttttg
     1501 cctcgcgcct ctctctctcg ccattttcat tgcgatttaa tttcaatggc cacccccttc
     1561 caccgccacc ctcatcccaa agttggaccc aatcctttgt cgtctctgaa ggcag
//
LOCUS       KCVB01000639            1494 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30241pri ultra-conserved element locus
            uce-105, sequence.
ACCESSION   KCVB01000639
VERSION     KCVB01000639.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30241pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1494
                     /note="ultra-conserved element locus uce-105"
BASE COUNT          456 a          251 c          307 g          480 t
ORIGIN      
        1 tttcttccaa caatgaaagc ttatttgtag tttttctatt gctcatggtg tatatctttg
       61 aaatgtgggt tcagttaatg tggaagtcat ccatcattga cataatctta gttttgttga
      121 actacgccta actggcatat aatattggcc ttggcctcat tgtgttgcac aataaagtac
      181 aatatctgtg gctcgaagaa aatgtaattt gatgatagta tgaaaggcaa cagtttgtgt
      241 tactacagta cagccttctg tgctttttca atatttgtgc aatatctctg gttctgagaa
      301 agctgtaatg taatatcatg ggaaggcctt gtatatgctt ctctgtaatg atgaaatgcg
      361 gcactctgca gcacacagtc ttttgtgtgt cttgacctgt ctgcagtata ctgtaatatg
      421 aaaccgcatc ttctgatctc tgtcggtgca agatgaatgt aaaggctgct gttaagtatg
      481 tcactgatta tttttttgtc actcgctgca aggtggttca gtgtaaccac actgttcaag
      541 ggatgaaccg agaacaacct ccatcttttt cttctgtagg ggatagattg cattctgatg
      601 caaaattcat tttgttaaca gcaatgctct ttattccact cagcagtcaa tccttcattt
      661 tgagaggcct gagcaagaga attacatgat tcatcaagca ggctactcca ataaattgga
      721 tagctgatac aaccatcaaa attgccatta aatgaattgt aagtgtgaaa tagaatggtg
      781 atatgtctga catagcacag taataaatgt agtgcgggcg agtggtgagt ttctaggaaa
      841 tagtagtaac tttctacttt gaagtggtct aataatatgt ttacacttag caatatagta
      901 caaaaaagag taattaccaa ggaatgggaa ctaaagaaat caagagctaa ctgttaatta
      961 ctgagtagct aattaatagc taatggcact tcctgaaaac tgggaacagg ctgcaagaca
     1021 ctctgttcac caaccagtgt ctaatgattt gttactagct atctgtgaat ctgcactcta
     1081 agcactttct taatgaatta ttcccaattt actcaaaatt atgtagtaaa tggcaccaaa
     1141 gcctatagat aataattact gctctattat gaacttgagt agaggcaaat ccttgtgaac
     1201 tgagaaaaag ctgctaaatt ttaaagatgg agtacgagac ttttacacat aaaggattaa
     1261 tagtgattaa gcctgtaact gtcagggaaa atctctctgt atttcacaat attctggagt
     1321 atttaaatct ggtgtctgtg gcggcattct tgtgatagcg caacagtggt gatgagtttt
     1381 acatcaacca ccaaagactg gttttgccag agtagaagag aggaagagag taggctacaa
     1441 cattgaggag catggcaggg tctatcatgt catgtttgtg tattaattct cact
//
LOCUS       KCVB01000640            1372 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30288pri ultra-conserved element locus
            uce-946, sequence.
ACCESSION   KCVB01000640
VERSION     KCVB01000640.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30288pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1372
                     /note="ultra-conserved element locus uce-946"
BASE COUNT          430 a          284 c          277 g          381 t
ORIGIN      
        1 ctgttagtca gtatgtgaga tcggtctcca tgtgctattt cagtccgctg tcagtttttg
       61 gtggatgcac atgcaaagtt ttgaatttta ttacatgcag cagagcagag aaactacatg
      121 caaagaaatg tgaagttgtg caggcatgca gcacatgaac acacagctcc acagtgatgt
      181 gctcgccgat actcatgttt attatctggt tggtgttggg gaaaggctta gttgcaggtc
      241 agaggtcacg gctgtgggat ttcatggcta aagcagagat agggaccact tgtgaaagag
      301 cgtggcctcg cagacgtgcc aaaacaaaga ggacaccaaa tgaacacacc ggacagacag
      361 agaggaataa caagaaacaa gtttgcagaa aaaagagaga ttatatgtca atgaagaaaa
      421 gtccaaacca agaaatctta acagactaac cataaaagaa ctgcatccag cacaaaacac
      481 acaaaaaaag tgaaaatact aatattattt agtgtcctta ttttcctatc tccgtgctcc
      541 cccagatttg catctgtttg cgctctccat ttgtctttgc tggtaaacac aatttaatat
      601 tagaataaat tacaaatgga agactgatgc ccgggataga tttttcttgc aggaagttca
      661 ggcaccgcca acattaatgt ccctcgccac cgttggaggc ataaatctct tttttcctct
      721 ttttgccttg aatcatatag ggatattgaa ttactcccct gctccgcaca catagaggca
      781 ttgtgcatta cattgactga taagctgtca ccagcagttt aacttagaga cattcaaaga
      841 gtaatacacc agttgaggat aaactaaccc ccgtcccctt agagtaagct atggcgctgt
      901 caccatgaaa aatacgacgg gggcatttca tataacatgc agcatctggc atctgaaaat
      961 agaggaaaat acctctgaac aatgcttggt ttatggctgt tttcatgcgg ggcgacagtt
     1021 ttacccttga acataaggga aagcttggat cagagaacca aagcaatata aaaagctgat
     1081 atacaagctt cactgtaatc ttttgactct ggaggaagga gggtaccatt tttgaatcag
     1141 agaacttgag ttactaatat aaaacaatgc acttcatttt accaacaacc attattatcc
     1201 tatttttcct cgttacaaca ccaccagcgg ttatattctg agctcatgtg tgtgcaaacc
     1261 ttcataactc ctttgatgga tcagtcttat ttttttttac ctctttacca tatttatgag
     1321 agcagctaaa gggccttttc atgctacaat ctgggaacaa ccattaacgc tt
//
LOCUS       KCVB01000641            1453 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30331pri ultra-conserved element locus
            uce-140, sequence.
ACCESSION   KCVB01000641
VERSION     KCVB01000641.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30331pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1453
                     /note="ultra-conserved element locus uce-140"
BASE COUNT          449 a          287 c          278 g          439 t
ORIGIN      
        1 tttttttttt actgtgcttc aaatagtgtc ataaatgttt cagaaccagc aatacaatat
       61 ggaaacaaaa aaaaggataa atactcaact ccccgcaatg gggataaggc cccaaatatg
      121 ttttggaaac ataatcagca tcatagcagc tctgtgtgtc tgtttacatt gtggcctcta
      181 ccatcgctcc cctctcctcc aacaaacaag aggatggggg gatggaggag aggaataata
      241 aaaaaaaagc aagaaatatc ttttgcatat tattttaagt aactgttcaa atttcatttc
      301 agaatggtaa tccacaaaac aaatatgtag ctgcatattt taaaaaggga ggctccgctc
      361 gcttcaattt cctattgtgg cgttcggcgt gtgtttgctc tttgtgtctg tttgaattga
      421 tttgcacagc tcggcagaca agggctgaat gctcagacca agaatccata atttcatatg
      481 atctgatagt tctgcagtat tagattgatt ttttcccctc tccctctctc cctctctttg
      541 gtgttgatgt taacaaacaa gctgaggtac ctgcgctcat ggaaccagag agcaaagaga
      601 ggcattttgt tcaggctaag gtgataatgt ggagatgggg acatgtttgt ttgaggaagg
      661 ggatatgttt gttatgtgtt gttttctgag gaggtacttc ccatctagcc tgtttagctg
      721 gcggggctgt ggtgtctgta gccacacaat atccaggact ggagggttaa cacaatgctt
      781 atctacagct ggttaagtct gcacacaatg cccatactgc cattacttca tatgcacaag
      841 tgcattagtc ctgacaactt ctagtagatg aatagattag atattggtta tgatgggaaa
      901 taagccatca aaccacaata caggtttgca acaatgttgg aggggaaaaa agagtagagc
      961 accgtctgtc ctgctaagat aacctcttcc caactcatat atcaaagcac tttaatcgta
     1021 atttaagact gtgccatttc actgtggaca ctcacttaca ctctccctaa tcagaatcac
     1081 tcattcaaac cttatcttac aaaatattgg cacacaaccc tgtctcctaa aaattaagtt
     1141 tatacattat acgtttatac aatacatctc tggagaaaac atgactaatc tgaactacat
     1201 acgcatacaa tgttcttatc cagttaagtg ttattttctt gtaccaaaca gaaatcatgg
     1261 ttacaaagtt acatggttac atgagtgtac aaagcacata agtttgacac tggagaacaa
     1321 ggttatgtcc aacagaatga tacaaatagg ttttctaaac tggtccccat atttggcagg
     1381 acaacatcat ttgttagtgc cttttaaaag tattacaaat cacctttgca aaaactaaaa
     1441 gccttttctg atc
//
LOCUS       KCVB01000642            1675 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30375pri ultra-conserved element locus
            uce-903, sequence.
ACCESSION   KCVB01000642
VERSION     KCVB01000642.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30375pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1675
                     /note="ultra-conserved element locus uce-903"
BASE COUNT          510 a          352 c          366 g          447 t
ORIGIN      
        1 gtctacagta ttgtctgcaa ggttaatttt gaaacaggaa gtggacagac tacatgagaa
       61 atgtctgttt tgtggtcaaa aatctggtgt cagttgacca agaaacgttg tcacagctgt
      121 cacgagacaa gatgctaaac tgctttaaaa ccatgcaaca tgtgctgatt acactgtaca
      181 ccttgccttc tatatgtaca gtaaactgtg atcacacaag gatttaccac tacatagaga
      241 attagtgtat tgtgttgttt tgtgaggcac aaaatgaggc tgaactcagg aagagaaaac
      301 ccggcacaga ttataaacac tgcgaagcac aggaacataa agaaaggagg agagtcaaga
      361 attgattggc gatgatttct tgcatctggg caaagaaaga aataaaagaa agcaaaaatg
      421 gaggctataa tcatgttagt actccttccc actttccccc tcctctacac cggcccaatc
      481 cctccatttc agctccctcc atcttctgag tggtcatctg ttgattacac gttatattta
      541 cctctgctgt ctcactcttt cgctgcctca ctcaggtcct ggctggcata cacactcgca
      601 gcattcaggc atacatacat aaataacata catacataaa tatttaatca aataaataaa
      661 tcactcggcc cagcggccct ttctaatcaa gtgacatcaa tcagggagaa ctatgggtaa
      721 agaggccttg tgtctgacgc ctactgagcg cgcccagagc gctggagtag cgtctatttg
      781 agtccaaggg gggaaatata cgctccatgt cccattatag gagaagggct atcctttcgt
      841 cagcgcgccc attactcccc ctatcatttc cccaaatcta aaacctgttt tcaatcaaca
      901 caaccaaacg agtgcctgtc caagagagcg agggagagag agaaaaagga gagagagagc
      961 aaagtagaga aaacggtaga gatggacagt gagggtaggg gtagagggag agcgatcgag
     1021 ggctctttcc ttctttcttc cagtgattgg agcgttttgc attctgtatg tgtgtgtgat
     1081 tgaatccgtc tcctaaatta gggctgtcga ctcgaggagc tgaatgtcct taacccccct
     1141 ccccatatcc aatgcccatc ctctgctact tgtgtgtgtc acatcattgg ggaatattgt
     1201 aagtgtagcc tgggtaaaag gtttatgttg agccattgat tgctaatgct atgttgtatg
     1261 tgtatttgtg aggaagcatt gttaggacat caaaattcaa actttagtag cgtttctact
     1321 aacttgattt ctgtatctga aggcggtact gcagaatcat aaagacggca aaaaaggaat
     1381 aaatttaaat ttaataaagc aggcctgttt tcacaactag tgctgcagaa aactttagaa
     1441 attaatcccg tcacaaatag aaaaagactt tgaaagttgg gtacaagcaa cgtgttaaca
     1501 tgtatgtatg tgcatagcct tacttttctc ttcaccctgt agcactttgg cgactgcata
     1561 ctcccagtcg aagatgaacc tgtagaagca gagctggttg tacagggcct tctcagagta
     1621 ctgtagagaa aacacacaga tgaacacaga ttaaaacatt gctactttga caaaa
//
LOCUS       KCVB01000643            1511 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30419pri ultra-conserved element locus
            uce-1273, sequence.
ACCESSION   KCVB01000643
VERSION     KCVB01000643.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30419pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1511
                     /note="ultra-conserved element locus uce-1273"
BASE COUNT          404 a          316 c          319 g          472 t
ORIGIN      
        1 caaacgcgct ctgaagttta aggccctcat aaaactttat tgcccctttt ccaccacagg
       61 cagactccgt ttcctgtttt gtcaggaaag gaagggacgc actagcctgt gataacattc
      121 gcccacaaca atatggatgc aaaactttgt tttcctgtgg ccgcagactg acgcgccatc
      181 actgtcgagg ccatatggca acttcatgat atcgccctgc tctttaatcc tggcatcatt
      241 acactgtaat taaaacaaca ctatctgcta gtatagagga cataaaacca agccagttgc
      301 ttggtgtcta tattcagcag cagcttttgc agtagtggta gtatgacgca gtatagccca
      361 aggttaggag gtaatttcgc tgtagcacta cttaaagaaa ataaaaatca actcacctct
      421 caacctaata gcttcctatt caacttcctt gacggttatg agaaccggtc agcgcagcgt
      481 ccactttctc tgcgctctgt taggcctaac ctatacgtgg acaatgtatt gaatcccttt
      541 ttgtatgtct ttcttttagg gtattttaaa gtttacaagg ttacatatgc caattgcccc
      601 aagcagggcc tgtgaatggt gcataggaag cacgtgatgt catttaagtg ggttttatgg
      661 cctggaagag ctgacaaacc ttcgatatat acacatcata tataatctta actgtccgga
      721 atcgcagctg ctggcttgcc cttatctgag gaagaaaggg ctttgtggca gtgaggatgc
      781 agtacctttc tccggactgt gcagtcgggt ggtgcggagc actgagagct gctgcagtaa
      841 gttgcttttt taactttgcc tacaggcctg cgactgtgcc gttatttcac tgcgagcgaa
      901 tattgcttat gttttatatt agtgtgactt gggatgcatg gatgttaaat tgattaactt
      961 tagaattaac ggtatttatt gggagagaat acgcgtttta taattaggcc tattgaataa
     1021 tgatgttttt gaaataatcg gaaaaatgtt agttttgaga tatttttgaa aggatatttg
     1081 gttttgcata ttttgtccga taaaatgtgg ttataaggaa ataccgccca tggtcttttt
     1141 ggattgtcat tagggttatt ttaagcagca taatcttgaa tttaaatttg tatcagattt
     1201 tgatctcagg aggtaaaaca agaagtggat gttttaaagc ctctagacag ctttatgaca
     1261 caaagaagca ggccgtttga atgttttgca tgttattcct gtcagtacga gagtaggaaa
     1321 tatttaaaag aaaggggact gtgaggaaaa tgaaagtcaa ccagaactat caaaaattat
     1381 gtggcgtcca gcttcaccac ccaccccacc ctctctctct ctgtgtctcc ctctctgtct
     1441 ccccctcgct ctctctcttc ccttctctct ctaaaacatg cacatactga ctctctcttg
     1501 cacagctgtc t
//
LOCUS       KCVB01000644            1855 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30460pri ultra-conserved element locus
            uce-529, sequence.
ACCESSION   KCVB01000644
VERSION     KCVB01000644.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30460pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1855
                     /note="ultra-conserved element locus uce-529"
BASE COUNT          525 a          334 c          381 g          615 t
ORIGIN      
        1 atctaagtca gtggtcctcg aaaagtgggg ctcaagaatc ctcaaaggtc cgcaaaggcc
       61 atgcaggggt tccccactaa caagacgaag aattgtttaa tttcactgtg attttattaa
      121 cctgaaatta ttccaattag agagcatatg tttgaacaaa ggtttcactt gtatcgccat
      181 tagcgccaca catataacag gtttacaatg cttttctaaa tcaataacaa caaaaaaaag
      241 ccatattggg agtccctggg actaaattca attaaatggg gacccttggc ataaggtgtg
      301 tccatttggg ggtccttgac atgaaaatgc tggagaagcc ctgatctaag tgactgaggc
      361 aaagggctgc attgacagtc tttgtttctt ttttctcttc tctgcttttt tcccttctcc
      421 ctctctttct ctcagtctcc ctctctccct ctctctgtgt ctctctcttt aaaagggagc
      481 ggaagagtgg gctgataccg gtctcatgtt gcgtatggat tttcgtttat ataatcgatt
      541 gaaatgtccc aatacattga ttttgggttc agtgttttac aaacaagcgt ttatgggtct
      601 cgattttatg ttagggatga ttccagtgtg acaacggtgc gcaaagaagt cggagttgtt
      661 gatttttaaa taaatgatct aaatacaaac aaataaatat ctattgtttc atgtataaaa
      721 aaataaaaaa tgactggatg gggggaggca aagaagaagg gaaacagggt gaaaaagaca
      781 caatattgtc ctgcctctct ccttaatcaa ataaagtggt gttaaaaaaa acacacacac
      841 gcacaaaagc atttccttcc ctctgaattt ctccttgaat tatatgccat cactagtgtg
      901 tgtgattgcg gaatattaaa ttaaagtggg ataagtccgc accgaggcgg gctgcgtcca
      961 atccgcaact catttcccct gttgtagcac cgcatcactg cgctcctctc ttttcaatga
     1021 caatgtgcca aatctgggca ttttgaaaac actttccata tgctattatc ggatcgctac
     1081 tgagcaagag aaagacagaa aagaagaaaa acggaatgtt ttataccaaa ggtaccgaga
     1141 gtctacattt ttatttatct tctctttcaa tggcttatta tactttatgc cttttgggga
     1201 tttaaaagag gtaagaggag gtaaatttga agtttttata acgattgcat atttatggac
     1261 cattgttgga tggctttagg gctgagtgtg aaaacgtcga gagagtttat gttgagcata
     1321 tatgggcaaa aaacttcctt ttgtttcact gcttttactg cgttttcctc atttactgct
     1381 aaagtgtaaa ttgtttttgt ccacttctct gtggagtgat ttgtcagcaa actagttgaa
     1441 gaaacatttt tcttagacat tcatttcact gaaatcacag aaataattct gtctttttga
     1501 tttatttgca attaattgat tttgcaactc tgcaccttaa ttcaacacag gtgacaactt
     1561 gccagtgact tttacattta gtggatttct ctgtgtgaga tttttctttt actgtcgtca
     1621 aaatacagtt ttaattattg cacatatttt gaaggaaaga ttttaagcgc atttaaattt
     1681 ggcttaatta ttctggagtg agtggcaaaa tctcgtaccg atgtcatgcg ttgaaagagt
     1741 tacacttaac ttcttattgt ctgttttatg ggcatttata aagcatgttt gtgcgtgtgt
     1801 ttgctgaacg agagttggcc agtgggtcac tgtggcaggt tgcgggtcag agctg
//
LOCUS       KCVB01000645            1465 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30507pri ultra-conserved element locus
            uce-204, sequence.
ACCESSION   KCVB01000645
VERSION     KCVB01000645.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30507pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1465
                     /note="ultra-conserved element locus uce-204"
BASE COUNT          445 a          305 c          304 g          411 t
ORIGIN      
        1 atgacctcgc tacatgacct gaggcagtgg aaatgaagca gaccaacttt gtaatttctc
       61 tgaattgaca tctgaccaga gaggaaatct ggcccagtat ggcttaaatt atttcacttc
      121 catttttcat gcctcgccgg agatgggaag ccgcactgta aaccaaggtc aaataatcaa
      181 agtgtttgtt catacatggt aaacatatca aatgagcaac tgtcacttgc ctattagatc
      241 atcattaacg gtaaaacaat gctgtgtaca tgtgacgtat tacactgctg attttgctgt
      301 tgataaatgc tccaaatgat ttgtcagaaa atcaaacttc ctttgacaaa aacctgcttc
      361 aacacaaaat tttcacaaca ttttcatttg tccttcattt tccttctttt tcttaacaga
      421 acaagcttac acaagtcaga cataaacaca cacacacaca cacacacata tacacacaca
      481 catggtaatg gagatgtaac tggaagagtt ctcgcggcat atggatgcca tttgtaagct
      541 gcactgagaa tgtataggtc agtcttaccc tttggtatct ctctatccat ctgttttcac
      601 attacttgca aattatagtg tcctccagac ccaaatgaac ataaatcagg ctggtgaaag
      661 tgcagaagtt ttcccatctg tcataaaaca atgatttaaa gtacgggttg tccaaacatg
      721 gcgctgtgaa ttcgccccgc tccccagcag aaggaacaca aagcaccatc tcatcggagt
      781 aatttatggc ctctgaggga gcggacggtg attagggctt aggccaccaa cagccagaaa
      841 aacagtagga gctccaataa ggacggctgg aaaacctaga ccaaatttat caaacaattc
      901 ggtgaaatct atttctgttt ggagaggaga gcgagatata tggcctgttt agtatactgg
      961 tacatctctg gaacatttgg tcatactgca tttgtgagtt ttggagattc tacattaaat
     1021 aatcgtagcc cgggacatgt gatgtgttgg tcagctgagg tgcccaaagc caaataaaat
     1081 atacaacccg acgttcctgg agtttaaaca acatattcgt gtaaatgatg tcaccgcgtc
     1141 tctataaaat tgtttctttt aaaggtggag ttttaggttg aaagcggctg tattcaacag
     1201 tggctgtggg catttggcat ccacaaacaa atgctggtct taaccgcctt taccttaata
     1261 gcctttttaa aaactgaaag gcatacatgg taaccaaaac tgcacagtgg gacatggctt
     1321 cctgtatggt cagttaatat attatgatta gcacagtacc accgtcagcg ggaggaaatg
     1381 caggggtagg gcgcttgcgt taacaatagg gtactgttga tgtcgggttg ggagactccc
     1441 aattaatact tgtgcttatt gacac
//
LOCUS       KCVB01000646            1627 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30546pri ultra-conserved element locus
            uce-690, sequence.
ACCESSION   KCVB01000646
VERSION     KCVB01000646.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30546pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1627
                     /note="ultra-conserved element locus uce-690"
BASE COUNT          471 a          325 c          345 g          486 t
ORIGIN      
        1 caaagatatt tatgagtcaa tatggagtta tttattactt aaagtgtgag cagctttgtt
       61 ctcacattga cgaggcagcc aaacacttga gagttccaaa aagacggtta taaatctgca
      121 caagatgact cattcaggtc ccctgtctgt gtcaatttta aacacattgt ttgactttac
      181 ctgcagtctc ctgcaggaac aatggttgtc aaacataatt tagactgaat aaatcctcct
      241 tgactgtacg tcagtggaca cctttaaact gggcacttgg agtttgaatt tgagggcgtt
      301 attgtttcat gttgtcctgt ggcgggcaga aaggctgtga ctcagctgtt tgcatgcaac
      361 attggagaat aatggatgtg aaggcacaca ccaaacacgg cctttaaaaa tgcatgagcg
      421 ccccccaaaa acctccctct ccctccacag aatagagcta tcactggtgc ctgttatctc
      481 cccgaacccc caggtagaaa atggtaatga taccacttaa catcgctgca catatggagg
      541 gaaaatggaa atgccaacag cacctcaact ataaagcaaa tcaaattgaa atattaatcc
      601 gctgattaat ataacattat gagagcccat tctctttgtt gttaaacaac aaagggttgc
      661 aaaataagca actgcctaag aggtaagggg tttaaaagag tcccacagag gtgctaaagc
      721 ccctttcaaa catcattact ctgcataatg tattcccttc cacagtgctg agatttgata
      781 ttgatgttta ctcatttatt ttttaattaa cgttcgttgt taatgaattc tttgatgaga
      841 ggcattttgt aaatcattat ccttggaaga ggaaaaaagg gatgcattgc tcgtgcccct
      901 tgtctttgtg acagaagaaa aaatgctagg tgtaagggga aaaagttgca cacgagaagt
      961 agggtcattg tttaaagttt gttttttgat gcgttgattt tttttctccc cccatcgcct
     1021 ccctcgtcgc tgtttttctg attgcatctt ctgtctacag ggatgagcgc agggagctga
     1081 cagggactcg tgcaggaggc tctgtgtgtg tgagagagag ccggtgtgga tacttagcta
     1141 attacaggaa tataactcaa ggctttcaga gagtagctcc agtagagagg aggggtgcag
     1201 ttacatctat ggagcccttg gtggaactct gtccttcccc tctttaatat tgatcactgg
     1261 ggatttcttg gaggacacaa agtaggctca gaggctggga aagtttgttg gaccaacagt
     1321 tgctgtttct cacaagtgtc acaaacagcg actccttttt taatcacgag ccttgtgagc
     1381 gatctgctgt ccttcatctg tcttgcaatg cttataacat caagtttgcc ttgaaccagg
     1441 tcacagatta ttatcagagt gcctcttcac tgttttcatg caattaaaag ttggtattgc
     1501 tgcaattttt ttaaggaaat cttgcaaatt atgtaattat tttccttaat tgtcaacaaa
     1561 tccaaatgaa aaaaacaaag ccaacaatga attgatccta caaacatgta tcgtctttgg
     1621 aaccaaa
//
LOCUS       KCVB01000647            1528 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30591pri ultra-conserved element locus
            uce-471, sequence.
ACCESSION   KCVB01000647
VERSION     KCVB01000647.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30591pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1528
                     /note="ultra-conserved element locus uce-471"
BASE COUNT          518 a          290 c          292 g          428 t
ORIGIN      
        1 gaagaaaatt gcaaaatagg ttaaataaga gtcttaaaaa aagagagaga aaggttttgg
       61 gataagaaaa taacgtttgt gagcagagta cattgtgtga atgaaagtta atgttcaggg
      121 ctatgaagac agagacatgg ccgtgggtgc tatgttgtga ggaataacat gcacagtgaa
      181 gggtaaacgc acgcttcttt ggagatcctg gaccggaaag aagcctgagg gagctcaggg
      241 gcttgtggga tacgggctgt attcaacctt tctctgcagc cacacacacc acgacaagag
      301 gggacgatgg cgcttttaca gtatgcaatg ataagcactg actcatttgt tcacacataa
      361 caaatatatt aactaaacat actgaagtaa atgactgact catgggaaac atcagaaatg
      421 attgaaacac tgtagtcatt tgttttcagc cacattatta agacaataaa tgtattattg
      481 tatgtacaaa caatttatat tacagcattt tatcagttgt ttaattaaaa gtattcataa
      541 tttttaaacc acaactgata atatataaaa caagcaagtc taaaacaatt ttctgatatc
      601 acacatttaa tttttaacag aacaaacatt ttaattagct aacatgagct cagccacaag
      661 taaatctaaa gtgattattc ctgccctcag agaaaggctg tctccctgcg ctctgtcaaa
      721 aggtcaatta tggccccaaa ccggcatgca gtgacagtga cctggaagcg ctactgatga
      781 ccccacatcc aggggcccaa aagagttgat catctaacat gcaggctgcc cggagccatg
      841 gaaatctgac atttgtcctt attcaaatga acacaaacaa tatgcatctc atgcctctct
      901 ctaacctgtg ccaaatctaa ggcataggga ccagcagtct taatgcattg tcctccgatc
      961 caagtccatg cccttccctg tgaatacagc cgcacggcaa tcacacagag cctgcagtaa
     1021 gagcgcacag cgaaaaggct ctgtcttcac cagcatctac actaacacag gtcattatag
     1081 ttctagtctg cagtgctggg gtcacagaac gaaatagggt cacacagtaa aagaaaatgt
     1141 tgtggctgag aacaattcca ggtagagcaa aataagaaat gactgattaa gattttcttt
     1201 gtagatatta ttcatgctgt tttatccttt tgcatttcat gctaaaggat aaaggttaaa
     1261 gaagttacag gatgaggcag gtgttattct ttatttttct tactatcaac aaatcatatg
     1321 aaaagtccca aaccaacaat acataagtct atttcttaat actttctgac ttccctaaat
     1381 ctatagaaca cactgtcatt catttcttaa gatgtatatc ttaaaaagtg ctaaaataat
     1441 atacttttat tttaacaaat gttaagtaat ttcctttaac aggaagctga gcagagctgg
     1501 acattgctgt gttttgtaaa tattagtt
//
LOCUS       KCVB01000648            1372 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30631pri ultra-conserved element locus
            uce-434, sequence.
ACCESSION   KCVB01000648
VERSION     KCVB01000648.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30631pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1372
                     /note="ultra-conserved element locus uce-434"
BASE COUNT          408 a          281 c          228 g          455 t
ORIGIN      
        1 cctttacctt ggaaacattt ggaataatat aagtacacaa caacaaaatt tacaacaaaa
       61 gatatagacc tttttagaca tttaatgcag aaatacataa aagtacattc tatatgttta
      121 tgaaagatgc agtaatgttg tgtgactaat tcatttcttc cagatgagag aagcaggcaa
      181 acatgtcgtc caaataaatg cagcaaactc cacaaataag ccagctttgt agcaatatgc
      241 tgacacatga agcaagactt caattaataa tgaatagaaa aacgaagtaa aggtcttgtg
      301 tttgtccaat taaacaacat gaaatatgaa taacaattaa ttatggtttg cttataggct
      361 ttaaacacca ccactgtaac atgggcatct ttgaagtcat acattttaaa gtcacagttc
      421 acccacatta ttatcaaata ggttttttaa ctacgaggtt cattaaatgt tctgcacatg
      481 agctccagga ctgtattgac gtaacaatca ataattataa taatcacagc ctttgtgctc
      541 ctctttgttc ttctcttgta agtttgtttc accccttctc aatgtctagg ctgtttaaat
      601 ctaatctagc tgaacacatt actctgctca cacagccccc agctgaatgt agcaactcat
      661 aaaatcccaa tatttcagcc atattgattc ctctggtaaa attggtttga gctatgaatg
      721 tgctgcagca ctgtacactg aaatgtttag cagcctgggg ggaaagaacg agggtgacct
      781 tgaatgtttt ttcccctctc ttactttcat atcagttttt ctcattctgc ctttgagcgc
      841 tgtttcatgg tttttctgat atcctcgggt gagctgtaac ttgagtgacc tatggcgcag
      901 tattcacatt ctcttaacct gaattacact ctctattcat gccttctcaa aagagaaaaa
      961 tatttggatt tgctcctaac tttgatggag gaaatgacat gaagcgattc ttgtttgatc
     1021 tccggttcag atcatttata tgacggtttc tctgcttcct ttcatctttt tagctccttc
     1081 agcaagagtt cactcagatg gaaagtacgt tatgtgcttg cttgtgatcc ctgaccagga
     1141 ttttaatagg atgttgctgc gtttctggta cagcacactg actatactct acactttggt
     1201 tatatggaat gtagagacag ctatgagcct ctcataatgg aataaccctc ttttaatgtg
     1261 ttatccttcc tgtgacacct caaccccatt cctttacaat aggaataaca gtagtttttg
     1321 cttcccttac actttatccc cctcttaact acattagtag agacaactga at
//
LOCUS       KCVB01000649            1324 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30674pri ultra-conserved element locus
            uce-1092, sequence.
ACCESSION   KCVB01000649
VERSION     KCVB01000649.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30674pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1324
                     /note="ultra-conserved element locus uce-1092"
BASE COUNT          396 a          263 c          264 g          401 t
ORIGIN      
        1 agagggggct tcaccctgag tcgggctaat caagggaaac acccccttcc aagagggagg
       61 gcgtctacag acagattgag cccaagttac cccgtaatta ccctgccact gcccctgtgc
      121 tgcaagctgc tgcatctgaa cccagtcagc agaggaacag acactttggt gattactttc
      181 caatctgttt aacagtgaac aacaacaacc tatgtgcttc cattacgctc ataaaccaca
      241 actttttttt tcctccctga aaacattttt aagggttcag agcaccaggc attttgttat
      301 gcttcctacg gtaacacagt gcaaatgtct cttgccgcgg gtttcgatta tttccaactt
      361 ggtgcagtcc ctggggggaa gttctcgggg ctttctgaat tttattaaag cctcaacagc
      421 tgattacttg gagtgaggtg cacaggttgg tgcattttag gagtggagtg gaggcctgac
      481 agggtcatgt ttaacatgtc cacaggcaat caattcacct taacccctcc tggtactcta
      541 gccctctcca cctaagctgt ccttaataac caggcaatgg cgaattactg ataaggcact
      601 ggggtctctg acctatatat ccctctctgt acgtattgga agaatccatc ctgaaagaaa
      661 tgtacattta taatgcattg aaatggcaga attaaacaat caaacattgc acagctgtga
      721 aattcacttt gttcaattca gtttgctgta cacggaggat ttgtttggtg tttgctgtgt
      781 gaaacatttg gccaaataaa cggaaataca tgcagcacat gaagggttga gtgaaaatga
      841 ataaaatcct acataagcac ttaatgagac atagcctaat attatgaaag cagcagagtc
      901 cacattctgt aatgtgtcag aaggctgctt tttctgcagt tacttcatgc caaacatcct
      961 ggaaattatc tataattcct tattttgaag tagaggtgtt gacgataact gtactgacaa
     1021 taactgtagt attaaaaatt gaatattgat attatgtaaa aaatacacgt atgataatat
     1081 cttgtaaaaa agacagagga tcggtttgtc atagcagtag tttgtcatat cattaaaaag
     1141 attctctctg cacgtccctg cacttttatt ttaagtgtca tttatttgcc aaaaaacaga
     1201 tgaaacagac attacacaaa ggtaaagatt agtttaactg cactattttt gtttttaagt
     1261 tttagataga tattgtgata atatcattat tatgaattgt tgttaccacg ataatcctgt
     1321 aatg
//
LOCUS       KCVB01000650             995 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30712pri ultra-conserved element locus
            uce-319, sequence.
ACCESSION   KCVB01000650
VERSION     KCVB01000650.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30712pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>995
                     /note="ultra-conserved element locus uce-319"
BASE COUNT          276 a          188 c          273 g          258 t
ORIGIN      
        1 agaactgagt cctaaaacac ttattttgtt agaacaaatg agggcaaatt cttttggaat
       61 aaattttgat atgtttgtct gtatcctaaa acaaggaaga agtaggcaat ttcctgcact
      121 agaataaaaa aacaatgcaa cttgataaca aaaaaatact aaaaaatctc acttcacttt
      181 cagacttttt cggctgtttt aagaaaatgt ggctttaaga caaaaaaaga cttaaaaatg
      241 aggtgaattt acaccctgac tgacacctgc tgagatacag atgcatgttg tcagtgcgtt
      301 cacctgtgca acgcctgaat gcaaaacaga ataacaagat aggacaacat gtaatctgaa
      361 agtgtaattt attaaacatc ctgttgaatt ttaatggcca tcacttgtaa agttgagttg
      421 ttttgggggt ttgagctgtg tggtaatgtc aacgccgtcc ttacacatga catacatgac
      481 caggaatgac ggcactgaca ggactcgcta tgccgtgtgt gtgtgtttgt gtgtgtgtgt
      541 gatcagccag gaagagcttt tgtccttggg ctgagagggc acctggctcc tcttcccgtc
      601 cttgtgatct ctctgttgcc tctctctcgt gccgctctat cccttctcta tctgtctctt
      661 cctctcttct ctcaagctcc gatcccttca tccccccatc tcgcgctctc cctctcctct
      721 gcccaaggcg atgccgtcct gcaggctttg atcagggggc cagggaggag agaggaagtg
      781 ggtcagaaag ttgtgctgga gactgcagga gaacaaggct ggaatatcga gtaggtgagg
      841 ctaggagagg gagagaggga gacatggggt aatctagtga gaaagtgata gagtgagaga
      901 gggtgagagt ggggggaaag aacagggagg gaataggtaa agaggaggag ggggggtgaa
      961 atggggcaac tgtgagaggc gtggaggggg ggggg
//
LOCUS       KCVB01000651            1390 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30759pri ultra-conserved element locus
            uce-770, sequence.
ACCESSION   KCVB01000651
VERSION     KCVB01000651.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30759pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1390
                     /note="ultra-conserved element locus uce-770"
BASE COUNT          420 a          321 c          281 g          368 t
ORIGIN      
        1 ttccccataa atgctgtcct agaacatgta tgctaccact cacaatttag tctactctga
       61 aaatagctaa aataataaac agataaaaat cattggatgt atacattgta tttttgttat
      121 atcaatgtaa tttatttgaa ataaaagttt gcagtcctcc ttatgctact tatagagaat
      181 ataaacagag aagaatagga taaaaaaata aaagaacaat aaatacaatt tttaatacac
      241 gtatatacac atacacccag gaaataattc tgatatatgc tgcttatact gttaaattat
      301 tgtagccatg acataaagct ataaataagg aaagggagaa atgacctaaa gttcataaaa
      361 taaataaata ataataaaat atcaatgatt taagtagctc aggtaagggg ccttgtacat
      421 gtacatgaag caatgggcct ttgtcacatc ctccacttgc agctgtccat tgtaaaacca
      481 cccaataaat tagcaaaaac aaacaaagct gtgatattta aaacaattat gatgttacac
      541 ttgatcataa cctgatggca cagctgtccc agagaccgcc ctgctgtcgc ctccagtatc
      601 tcggcctgtg gagactgaat caggtgtttg accttgagcg gcggagctga gctgagctga
      661 gctttggctt ggcgctcaga gctgtaatta cacacactaa cacagcgtag cctagaccac
      721 catgacacgc acacacacag acacacacac aaaccaaccc atttcacccc ttatcaaagc
      781 ctcagttcta agtgtcctat gaagtgtaat gttgtggaac agatacaggg attgtagcta
      841 gggtggttaa ctcctggatg ggatgcaaag aacactgctt tcatactcga tgaactgatg
      901 attataatga ggcaggcacc ccccctacac accctccaac ttctcccctc actccctccc
      961 ttcacctctg actcattctt gcttttcctt ttgtcctcgc tctctctctc tacagtggac
     1021 tgcgtcgatc ctcagtgcgg agggcacggc gtgtgtgttc gcggggagtg cgtgtgctcg
     1081 gctggctggg caggagtgag ctgtgatgac ccgctgccag cctgtcagga gcagtgttca
     1141 ggacatggca cctatctccc tgagtcagac acctgcgcct gccagcccaa ctggacggga
     1201 ccagactgct acactggtaa tacctgagga ttattgttca cccgcagaaa aaacactgaa
     1261 aatgcatcgc acatcaccgg tttgccagtg ttcttgtagc acgtgtggtc ttgccagata
     1321 ctattttctg gggatttgat agacatgaga aagaaatatg cattggagcc actctgtgaa
     1381 atgtgtctct
//
LOCUS       KCVB01000652            1627 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30803pri ultra-conserved element locus
            uce-735, sequence.
ACCESSION   KCVB01000652
VERSION     KCVB01000652.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30803pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1627
                     /note="ultra-conserved element locus uce-735"
BASE COUNT          463 a          307 c          350 g          507 t
ORIGIN      
        1 gtgtctttct agtctgacga ggataatggt gcgaaccttc agacagcctc tcctcaaagg
       61 tatgtttagt gttgcacttg attttataca tgaaaaatga tggatgtaga tgagcttgat
      121 agagaagttt acacccttga ctttcccacc tttgcacgct tggtcaatcg cagggaacac
      181 acaaagttgt catgtttgaa agattgttat ttttggaaaa actcaaactg atttggaccc
      241 tgattaccat cacattgtgc ttttttaagc cataccatac ctacaagttg taaaatacac
      301 gtttacccta cctgatgcag gagtctaatg tgactaatgg ttagacataa gttcagattt
      361 aagttcactt tagtttgcac cccccttaat ataactgctg gaaataaggt aggtcttttt
      421 cacaataaag cctttatttt aatgtatgta aagcagtgtt actgtttctt tagtataaaa
      481 gcatcttaaa ggataacagt aatgatgttt tttgttctct gtacacatgg ttctatatgt
      541 ccactgcagc ctctttatca gtcatctttt atcttttggc ctgtgcctat aggcagagag
      601 tgagcagcag ggggtgtaat tctgttctaa agcccctagt taggcatgaa tatattctta
      661 ttatagtcag tgctcccgga acaagacaac agagagcaaa cagaatgacc ttgaaatatt
      721 tatccccaac ctcgcttcgg agaattaagt ttggtgtcag gggcaactcg cagctatcca
      781 gctccacttt gtgtggaagc atgcatgccc atacacaccc acgcacaaac acacacaagt
      841 cctcctttag cgggtatgta gacagagaca aacggctcat gtaccatctt gcagtgttga
      901 atattaaagg cggtcaggga gacaaggctt tgattgggac aataaaggct gatgaacagg
      961 atcttagagc atgggagagg agagggtgtg tgtgtcacag tgcaacgaat accagccaaa
     1021 accctgaagt ggaagccagg agcttcccag cccagaatgt tatgtgtgta tgtttgtgtg
     1081 tgtgtgggtg tggaggcagt tagggagggt gaagaaggct aaaagatacc cagtccgagc
     1141 aaatatgggt caaattggtg gtaagaatga ggtaaatgtg tttagacttt aattgcactg
     1201 ttcttctgtt ttctgcttcc tcttgccttc agtttgtagc taaagtaaag tgatatatgg
     1261 ataatttcag tatattgcaa cctattttct gagttttggt catcatttgt caattatgaa
     1321 aacactgtac acaccagagt aactgctgtt attggacaca aatcaccatg tcagttgtca
     1381 gttttttttt cctcatgcga ttctgtgagc ccactgtggc ctttagcatg attagcttta
     1441 ttaatgtttc tgttacacat agcatccaga gttttatttt tttaagtgtg tactcatgct
     1501 gcacagcatt agaaagctta gattcttgtg atttgattga tgcatacctt ttaaagatac
     1561 aatcagctgc aggttcaatc aacatcttgg tcagactgac aaaaaaagtg aacacacaaa
     1621 gacagtc
//
LOCUS       KCVB01000653            1473 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30848pri ultra-conserved element locus
            uce-59, sequence.
ACCESSION   KCVB01000653
VERSION     KCVB01000653.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30848pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1473
                     /note="ultra-conserved element locus uce-59"
BASE COUNT          380 a          329 c          341 g          423 t
ORIGIN      
        1 gggctctgcc catttgctag atatcaatta ccttttttct gcttcctatt tattcccttc
       61 ttaggccaca ttggagatta atgtgcactt ctaaattacc acacatgcta gccatttatc
      121 agctccgaga acaatcctgc agattcatcc caacgctaag tggcagaata ttcatgaaat
      181 agggaaaaaa gaagtaattt tgtacaccct ttaatgataa aaaaaagaag gaatttctca
      241 ctctcgctca tttactttgt gagttttttg tcattgtttt cttgtgcggc cactcagaaa
      301 aggacaggct tcattgtgga ggaaaatcct tcttaattta gctgaaaaca tggacttata
      361 tttatgggtg gaaaacagtg cgatacatta cggtcataca atgtgataat ggtgatagca
      421 cggtgcattg tgcttgtttg taaagccgtg gtgcagaggc ccataataga cctgcattca
      481 atccctgact tcagtaatga gttgggtaaa cagagctttc aaaatgagtt tctaaatcag
      541 tgcatagagg cagaggagag ggaggaaaaa aaacaggggc tgggggcttt tggtcctcga
      601 caccctggct ttaatcaggc attaggaaaa gggagtgaag atgttatgaa aatgcggcgc
      661 tggcctttgt tcagcgtctg tcatggcatt gacttccctg acacaatgct acaatgcatg
      721 acggatcaaa gtaattaaag ttccaataat ttaccttcct ctgttgcgtg tcaagtctgc
      781 cagaccggct gctctcaggc ccttcattga tttcctcacc actatcagag cgcgatcctt
      841 aaagacagag agacacgagg gcaggctatg tgtgtgtgtg cgttttaata actttccatg
      901 ctgatataat agcaacgctt cagatgttta accattaatg atcacagaca gatgacggag
      961 atagagagac gagcgcgtct gaaccctcct cacccattcc ctccttgtca aatcactctt
     1021 ctcctgctcc tgtttgtgaa gtcgcctgtc aagagtgcgg cggcgtttga gcgcgcatta
     1081 actttcctcg ccggctcaga gtggtcaaat cggagctcgt cgacttcacc ttgttaaact
     1141 ttggaatccg ggagaaaact cggctttatc cgcaaatcca tgggagattc agacgccggt
     1201 atgtgaggat tgtgaaatca ttatcggtat acagaggagt aattctgtct gccgtctagg
     1261 cctgggattg atgtgtaatg cttaggaaag actccctgcg tagccgtgtg cgttcaccac
     1321 aacagtcaga ataaaaactt ccctcgctca tgcactcgag gttactctgt ctctgtcgtc
     1381 ctccctccct cgtttccccc ctctaaggca cgacaatgtg tcattgtcat tgtggttttg
     1441 cagcaggttt ctgcggtatg aggtggtggc ggt
//
LOCUS       KCVB01000654            1892 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30893pri ultra-conserved element locus
            uce-708, sequence.
ACCESSION   KCVB01000654
VERSION     KCVB01000654.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30893pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1892
                     /note="ultra-conserved element locus uce-708"
BASE COUNT          502 a          408 c          414 g          568 t
ORIGIN      
        1 ttatttatag tggcgattta atcatcaatt atagagtggg cagatcagaa aaaacagatg
       61 aaccacttta gtgttttagt gcgacagtga cagtgttgca gaacaaagat ctcagtgaaa
      121 gtgtttgttt ttgttattaa aaatctgcta caaacatgaa tctacaactt tcaccttttc
      181 ctctctttgt gtcgagtctg gtggctcgtg cagaatgtgt gtgtgaggtc atgacagctg
      241 acatgttcac agctgcttat ttacactgtt cactcatcca cagctaaatg aatgtgttat
      301 cacagaggag ctgtgttcag tgtgacgctc aggacacgga ggggacaagc acttgtgcat
      361 gtgacatttc actccctgtt gttttatcgc attaggtgag cgcccgcttc ctcgtttggt
      421 aagctaacat atgtattagc atgttgcatt cactgtcaag caaagctgtt aacctgacat
      481 ggaaagaggt tttggttttt tcgtttattt aaaatttatt cctggttttg aatttgactt
      541 taaccacaac tttcatcatt tttagagttt atgaaaacta attattgact caccttaact
      601 gcatcacatt ggttttaact gtgctggttt gatatttcac tgaatttctg aaggacatga
      661 atccgcccgc tttccataca gtaacagcaa ccaaaagagt tttattactg cggctgtgta
      721 tctcaccaca acaggtaaac tgagagagga cagttgtaga agaatgaagg agaaggagag
      781 acatgggaat aaaaagataa acagtttgac cttctgtttc tgccaatttc cagtggtcgc
      841 ccccctcctt agccttctct ttccctcttt tgccctcagc cctgtgtcgg acgaatcctt
      901 tttggaggga taaatagcac tgctgcgctt tgatagcccc agtcccaaac agcagctacc
      961 gataaataaa aggaactttc tccatttatc tctgtgagag agaagttcct attagggtcc
     1021 ccatagccac ggggcctttc cctccttcac tgcacagctt tgtagattgc ctgcttggtt
     1081 taaggtgcta ttgagtgctc accaggacaa ttacatgcca ggacccccct ccggaaatgt
     1141 ccctcccctc cgcttccctc gcccgtcctg tacctctgtg ccagtgttgc acgagatgct
     1201 ttccacgata ggactggatg ggaacggtgt gtgcggcgcg tcgtgtaatt gcgtgtgcat
     1261 gtgtgtgtgt gagctccctg ctggaagagg ggacagtggg ggtagagtga taatggccct
     1321 ctgaatggta tggaataaag tatcattgct tactgcctgg agtcagacag tgatgctaat
     1381 gcaaatggtt tttacctcca ccttcctgtc tggtataatg atagccccac aaacagtatg
     1441 tatgaagaaa cagagcgaag gaggtgtata aataaaaata gggcacacct cagtgaaaat
     1501 gactttttgt tgccttcagg gaacttaaga cacaccttta cactttccct ccgggcctcc
     1561 tctatttcct acttctcttg atattttttt tatcatttga taacacataa agtagagatt
     1621 tctgcaagga gcaatatagc agtgaaaaaa agaatcgtgc tgggtttcag aaagacttgg
     1681 ccctcgaccg ggcatgtcaa tctcatttcc aacagcctca caaaatcaag gtgttttcaa
     1741 cacataaaat gagatgtgtg tattggttat aaggaacacg agtccaaggc ttcaccttga
     1801 gaagatactg atattttcca gcatattgtt aaatcataaa cagccaattg gatgacactg
     1861 gtgtgctttc caaacacggc gtctcgctgg at
//
LOCUS       KCVB01000655            1160 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 30974pri ultra-conserved element locus
            uce-64, sequence.
ACCESSION   KCVB01000655
VERSION     KCVB01000655.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="30974pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1160
                     /note="ultra-conserved element locus uce-64"
BASE COUNT          338 a          301 c          201 g          320 t
ORIGIN      
        1 aaaaaaaaaa aaggctccac tctcacgcca aattacaact gacaccgctg atactgagcc
       61 ccattgttct taatgtcatg cttatggcaa gtgcacaggt aagtggcaca tgttgctttt
      121 tttttaaaca tggacaccgc acaaaaaaga acatacatac atgcataaaa tataaagccc
      181 tcactgccaa cacgcacagc cctccaagac agtcctcagc ctacatcaca tccacacagt
      241 gcgcgcagac acaatgtaat atttccttac ctctctcact gtaccggcat atcctgagcc
      301 gagaaatgcc acacaatctt ttttttttca cacaagcttt gtcaacatca gcctcatcag
      361 atatggggct cgatactgag tttctcaagt agccactgag tccaaccctt ccagcaatgc
      421 gttgccagac taaatagcaa ggcacgacta tttactggta atcatccttt atatgctctg
      481 tgtttccgtt gctacttacc attttctccc tacgctgtcc tggagataga agtgtcttaa
      541 tatccactca ctccaacaga tgctggtagg taatgtgtct tgtttgaagt catcatatga
      601 gaacttctgc tgtgctcagt agatcgccca ccacttcgct gacttatgaa tctaataacc
      661 ctttgcaata tcggcgtgct taaactctct taggcaacac cttggtggct ctacagtaaa
      721 gacaaatatc aacaagcctg ggttgtcaga aaagcttgta cagatatcta cgtaacaccc
      781 tcgcccggac aacgccatta ggggtcttac gtgattgaaa taagcgacgg caatccaagt
      841 ttatggggat actagttcct gactgatcgc tcgttcactg cctcaaatcc ttaaatccgc
      901 atcggacaac attttcagac acataaaact tatccaaaat aattaaataa ataaaacata
      961 tccttattca cgtgtttgtg ttatagacta taatagactc cctctttggc gatgggccat
     1021 aggcctatta cgcatacgca ccactggtca ttttgatttt tctaacacgg acgatgcaca
     1081 gccaggctgc cttttacaaa cacatagcac attaaaagcg cccctgtgtc tgcctctcaa
     1141 gttattgagg aacttgttgc
//
LOCUS       KCVB01000656            1811 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31015pri ultra-conserved element locus
            uce-21, sequence.
ACCESSION   KCVB01000656
VERSION     KCVB01000656.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31015pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1811
                     /note="ultra-conserved element locus uce-21"
BASE COUNT          400 a          407 c          431 g          573 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgtgtctg tgtgtgtgtt tatatctgtg tgtatgtgct
       61 ggaggaaggg agggaggcat caggggagaa gcgagagatg aagagaatga gcgtgccgct
      121 taggaaatgt cacactggga agatttgtag gttttgatcc tggcaaatta atgcaaaacc
      181 ccagtgcaag tatttgattt gggaaaactt tgggagcaga cttgtctctt aataataatt
      241 attgggttta cattaatttc ctcccctatt ctcttccaca ggagagctga tcaccactgc
      301 ttgtgagctg ggggtaagta ttagctgtat tgtttaatca attcactcat taactcttat
      361 aagcattaag gaagtttaat taagcatggg cagaaagcag attaatttac tttcagtcca
      421 caggtttttc cccttctttt ctatacactt attttactaa gttgcaagta gagaatgcta
      481 taatttgcaa cccgtttttc aaatccaatt aaagtcaatg gtaaggcgat ggcttttgta
      541 ggctttgcct tatgtacggt ataaggctta ttatatccac ccctcttttt ttgtgtgtgt
      601 gtaagccgcg aagaatgaac taaatcgggt taacagttat aataagtttt attatttaat
      661 aaagtttatt acgccgctgt gatcctttta gggaggctgt gtcggtgtca ctatgccagt
      721 aatcctgccg taagtgctgc agtccgtgtg gcctgcctgt ctgcctttca ggcctcccca
      781 gggcctcgca ggccgtccct gggccccagg ctgctggccc ccccttctga tccccagagc
      841 tccagagttc tgcttccaca tgctccctgt ggcctttgtc cctcagctgt ccttttgcaa
      901 aacgtctgtc cactcctgtc ggacacacag tcctttctcc acattctctg aactgatgct
      961 cattttagtg tagttaccta cctgtctggt acaaacactt caccaagctg tgtgctgagt
     1021 tgctattttt ttatctttct tttattctct acattacttc ttgtctttta ctccttgact
     1081 tcttctgtac ttcgtgctct atccccatcc catccctctg aaatacggtt aagttgtttc
     1141 tgtgtctgaa cgtaaccaag gagcctttag tgtatctgag gtgacaggtg agttagtcat
     1201 actctcacct ttcagctctg tgttcatttg aaaagctgcc ccgtctcgct gcttgcgtgg
     1261 ctcgtgttat cagtgccagg gaatgagtct gtgtttaatg actgagccct tatctgggtg
     1321 gaggagactt agcggctaat tgctgtgatt gctgagatcc cccaggcgag aatgactgcg
     1381 gctcggaggt ggtttggggg ttggggctgg cgagcaccac gtgctctact gcgggctcta
     1441 gggagctgga ctgtccttgg gaagaggcca gaggaaaaga agtgagaaga gggaggaaga
     1501 gagggagagg agggctctgg tatcatagcg aagtcacttt gcggtcatgc caggcgtcat
     1561 gtgtctgcct cttctctatt aactgcagct ctgcagcaca gcaatctact gaggtcagaa
     1621 gaacaactat atttgccagt ctatcagaaa gggatagtaa acacaacaca tcctactgct
     1681 ccttaacacc atctgcgcca tttggtggtt acctcctatg ttcgcattct agctctgcct
     1741 actgttatct ctagtataac ctctgcactt tcactttagc ctgctgtcca ctcaggcaca
     1801 tcttgaatta c
//
LOCUS       KCVB01000657             759 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31057pri ultra-conserved element locus
            uce-409, sequence.
ACCESSION   KCVB01000657
VERSION     KCVB01000657.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 759)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31057pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>759
                     /note="ultra-conserved element locus uce-409"
BASE COUNT          227 a          217 c          117 g          198 t
ORIGIN      
        1 ccatttaaat ctctctcttc tccatttaaa tctctctctt ctccatttaa atctctctct
       61 tctcccttta ctccgcctcc cccttttcta ccccccaccc cccaccccat ctacctcctg
      121 tgaggccgcc cacccctgag agtgattaaa cgctgctgaa gggccgttat gctaactaac
      181 acactaatca tttaatacac acattaccac aggggccctt gtggctcagt ctgctgacaa
      241 ggtcccaaaa cacacacaca cactcacgca cacatgccca ccaccccaca ttgcccacac
      301 ccccccaaca cacgcacaca cacaagaaaa tacaactctc taaagaaata gtgtgatcaa
      361 gtactaatta ggagattttt tcccccatca gcattaagtt taattccctg accagtcatg
      421 atcctgtttt atccacaaac cataaaaaaa atataagaaa tgttggttcg actgtcctct
      481 gcaaaggaat ttaagcagta gcacaggacg tttgaattta aaaaaaaaaa caacaactgc
      541 tcaattgctt acatgccatc ttgctctcac tgcccatgga gcagttgatg tagcagcctg
      601 tctgcccatg gccatggagc agctgcacta tctgttagtg aattcgagcg tgcgcagaag
      661 cttgtgcaac gcttgatgaa agggggaacc taataaattt taatcatgtt aaattaattg
      721 tcaaaaaatg aatgctctag ttcatgaata gaggtgtaa
//
LOCUS       KCVB01000658            1468 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31084pri ultra-conserved element locus
            uce-99, sequence.
ACCESSION   KCVB01000658
VERSION     KCVB01000658.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31084pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1468
                     /note="ultra-conserved element locus uce-99"
BASE COUNT          476 a          284 c          303 g          405 t
ORIGIN      
        1 aaaacaattc ttgctggtac gttgagcagc cttgctgttt tcactgagca agtgacactg
       61 ggagagtgag agagtgtgaa gcctctttaa ttgcctttgg tcacccacaa cgatgaaccg
      121 gagcgccacg cagcttagca aaggaaccgg gcagagggcc tgatagatat tttatcgccc
      181 aagttaacgg ttaatgaatt cccaaagtga aatctgtcac ttcaaaaata aagaggaagg
      241 caaattaaag ggagatagag agagacaggg gaagaaaggg actggagagg gtgacacagt
      301 tttaaaatgc cctatctttt ggccatcccc ggtttttcaa tggaataaag aaaactcagc
      361 ctctttcttc tcctcttttc ttcccccgct tcgttttttc tccctcccat cctcctatct
      421 ctcactccgc ttttctctga aaaaaattct catgaatgat ttatatgtgt cttgttatca
      481 gcaacgattt ctatagaccc caacgcattc accaagacat ttgtggcgta attgttaatt
      541 ttaatgccat aatggcttca gatgggagct ctgattagac atttattaca caattaaatg
      601 aaacatactc aagaatcaca aaagccttcc gtaattattt caggccatta ataaaatggg
      661 cattaaatta tattggacac aaaagcgaag tgaaatagat ttccattaga gcaatagagg
      721 cagagagggg taatagatcc tgcctgaatt cactctgaaa ttaattgttg cccagcctat
      781 ttccacacac agtggaataa atgaatgaga aaagagagga agagaagaac gagttggtgg
      841 ccgtgagagt gaagggaggg ggttacatta agagcctctg ataaaaacct tttgtttgac
      901 gtactacaat ggaccctcaa ttcaagagag agagagagag agagagagtc agaccaattt
      961 caatcttagt ccgtccctcc agagttctga gtcgaggtta tatccccttt tcaagttcgg
     1021 tttgctttca ataaacagct atcaaaggtc tacctgcgaa tgcatatatc taaatgtgac
     1081 acttattaga gagcaaagtg tgggtcagaa catgagcaaa agacaactgc catgttagaa
     1141 aataacagtg gaataaatgc atatgacagg aagtcaatct tttcttttct tttcttccat
     1201 taataaatga cctgccgggt ccgaagccga ttctctgacc ctttgacagc gagtcacctt
     1261 cctgctttaa catgatggag gtcaaactcc agagactaaa gattagctgt gattcaatca
     1321 aaatctttat ctttcaggaa aaaaaggtca agtttttcat aaaattataa gaaaacagtg
     1381 agaaaagata catggaagca gagctttgct gtaaataata taattgtgat aattattctg
     1441 tatgtaggcc cacagttaca aactaatc
//
LOCUS       KCVB01000659            1801 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31118pri ultra-conserved element locus
            uce-1316, sequence.
ACCESSION   KCVB01000659
VERSION     KCVB01000659.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31118pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1801
                     /note="ultra-conserved element locus uce-1316"
BASE COUNT          439 a          451 c          377 g          534 t
ORIGIN      
        1 ggagacccta tacagtgccc tgtaatggaa atggaacatc agggctgtgt gtgtgtctat
       61 gtgtgtatat cattagcagg gtgcatctgg ccattagggt cctagggtcg gtgttgtttt
      121 gctctcctcc tcactgtgtt tctgtcggtt atcctctaat gtgttactct gaccaccggc
      181 actctgtaag aggacaaaaa catttcctca ttgcctctca cacaagtgtg tgtgagtgag
      241 gtcgagactg atttctcaaa gcaggtagcg agtaatgaaa gacacactct aagccttcac
      301 gaattggcaa tcgagagagg agagaagact tcagaaataa gcactgttta ggggtgggaa
      361 gcgtcaaaac gcaccaatta ctatccccag acagttaccc ctccaaaaaa accacacaca
      421 ctgaaatcaa tggggacaca aactgaacaa tcagctgcaa ctagctatca acaatggagt
      481 ggaatatgca aaaatgccaa ttaaatgtgt tagcatacgc acagtttttt tctttctttt
      541 tttttttgta tgatatccca acatctgtgg cattagccat ggctctcttt atgtaaaata
      601 gaaacaggga aatgaatacg caatctacca ctgtgttttc atcattagtg cgctccaact
      661 cacggctctg ctggctccca agccgcctga gtggggagcg agaggaacat ttgtgtgccc
      721 tcgcttgcca aattaattct tgtcttgaca tcttaattct cagttcttgt gtgtggtcaa
      781 atcagcctac tatagaaaag caatcaagca attattccca aattatattt gcccaacaca
      841 cactgattgg ttattctttt cttattgagt aagctcatta attctgagca gcttgcttgt
      901 tatgcctctg ccattactcc tttcccctct catcccctct ctctctgtct ccctctcttt
      961 ctgtctctct caccccctgc tcttcatcat gcacactcac tcccggctct cactcccact
     1021 ccccccccct tcacgcactc ctctttcctt ctctctccaa cagatgcacc ctatcttcca
     1081 gccccagcct gctgatgttt tggagatgac atacactaca aaagccccat gatctgtggc
     1141 attgtctagt cggccaatta ctattcccat ctctgattaa ctttgatgtg gaacctaatg
     1201 agataaggca gcttatcaag gcagcagctg atagtggtct catttattcc tgtggctggc
     1261 accccagcaa gtgacaaaaa ctaatgagga ggagtttgta tgtaagctca ggtctaaggg
     1321 aagtgcaaaa ttgttcatcc gcagttcatc agctccagcc tgaaatggcc tcggcttcgt
     1381 cagcgtgcag caccgggaca ctccacataa cagcacagtg gctcgccgta gaacaagagc
     1441 cgccacgttt ttctctgtgt ctgacttcct ctctctcctc ctttctgtta acagctcctt
     1501 ttcccttctc caggctctaa tggtgcagct aattgctcag gttgtttagt tgtgaggaat
     1561 ggaggagtgt gtgtacaaag ctgtttcctt cgcctggtct ccgagaggtg aaaccttaat
     1621 tttccacttc tgccatctgt gtctattttt attttgtgtg tgtccgtgtg tgtgtgtgcg
     1681 tgtgtcctta tataatgtat gtgctggtgt gagtgcgcaa aaatgtgtga aagagggagg
     1741 caggtccgca cagagtgcac aagagattaa ttaatgtgac cagagtataa ttaccacgag
     1801 c
//
LOCUS       KCVB01000660            1013 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31148pri ultra-conserved element locus
            uce-361, sequence.
ACCESSION   KCVB01000660
VERSION     KCVB01000660.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31148pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1013
                     /note="ultra-conserved element locus uce-361"
BASE COUNT          336 a          198 c          212 g          267 t
ORIGIN      
        1 cccccccccc ccccccaaca ctctggaatt tttatgagat aggacaaaaa tgggcacttc
       61 ccttattaac atctcacagt ggaaatcaaa tatgcaagga aagtagtgta tctactaatt
      121 agtgctgaat tcataattac tgtgctcatt gatggagata catttgaata cgttcatttc
      181 ctcagggaga aaggcaggct gtccgggata gactgggaga aaaaaaaaat ccccttcagc
      241 ccccctccct tttcttttgg aaattaggga tccgtggcac atggcgatac taccagacta
      301 agatccgaag tgcccgcaat tttcttcaga tataattaat caagtgaagc atgtaaccta
      361 atgaatgtgt ttgatatccg ctcctattca aatatgggct ttatcgcgat gtgtgccgga
      421 attgcttaga gctatgcata gacagtgaca ttgcttggaa atgttcttca acagtttctc
      481 tttgcgtgta aacaatatcc atgcttgtca aggcatgaaa aaaaacagac agagagacac
      541 attttaattg cagtaaatta ataacaatta agccgcagga tgctgggtat cttgacagtg
      601 ctttgttgtt gccctggaga taatttcact gagtgccttg caagtctgac aagcaggtga
      661 aaaggtagaa aagagaaaaa aaaaacctga caatttcaca gagtggatta taagaacgat
      721 acaagacgag gctgcttctt ttttgcagtg gattaaagaa atatagattt aattaaccaa
      781 aacattgact ttggaagttg ctgacttgtt ttaagccttt gaccataaaa gttgacagat
      841 gagttcaaca ttcaatcgaa caactgtcca acatacacac caaaaaatga aaaatgtgaa
      901 agggaaggca cttgcagagc cgtgtgagta gacagataga caaacggaca ttgcaggaag
      961 aagatccata agtccttact gtgacctcag agtgtcagac cagcagagtc acg
//
LOCUS       KCVB01000661            1255 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31226pri ultra-conserved element locus
            uce-986, sequence.
ACCESSION   KCVB01000661
VERSION     KCVB01000661.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31226pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1255
                     /note="ultra-conserved element locus uce-986"
BASE COUNT          382 a          220 c          288 g          365 t
ORIGIN      
        1 tttttttctg taatgagaga ataatgtatg tgcttaaagt ctgttcagct ttacaaaatt
       61 gacttattag cagtttaaaa caaaattatt tatcattatt taaatatatc acagtcttaa
      121 agttgaagcg tgtttcccct tggctttatt tgcactatgt ttctcactta acagtgtttt
      181 aaactgacaa actcaccatt ttacatctgc ttaatagagc agaatattct gtgaatgtaa
      241 agaacaaggc acatattaaa tctaaacacg ctttagcaag tggagttctt tgaaaatagc
      301 ctttctctgc aatttccccg tgacatgaag atcacctcca atcctttgca tcttctcctc
      361 atctcttccc tctggatggg gaggtatgtg ggtgtattac attaccaaaa caaaaacaaa
      421 caacagcaat attaaacttt tgcaaccaca atagaggagg ggataaaaaa cagagggtcc
      481 tcgacttctt attaattatg caagattagt ctaattataa ttcagcaaat gaatgtgtgg
      541 cagaaggtgc gaggcgacgg ggctgggaga tttgcatgtt aagtgggcag ggggaagcgg
      601 ctaatgcatg ctaatatatg cgtcaatgtc tcgctttaat ttcagcattt gcagctgtgt
      661 gcgtccgaac tgaggggggt gaaaagttga ctcaatgggt ttttgggagg ggggcaaagg
      721 tgggggcgtc ggtgtattag agtgtgcatg catgcatgtg aatgtgtgtt tctgtgtgtg
      781 tgaggcatct ggtgtcatac aaacagggaa tgatgagaag aagtcaacaa acaaggtcaa
      841 gaaccctcag aaagtgcaca aagcctgaga tggatgtaga caacctctca attagggttg
      901 tgattttgct atctgaggcc tcaccacaag tttcatcacg tcagtcagtc aaatgaatga
      961 tgactgctgg ttagaggaac atgaagtggc caagaggcaa gtggcaagga aacgattgtg
     1021 gttaaaccag gggcgtggtt tttgtgctcg ctgtgctgac ttaccccctt acacataaat
     1081 gctatagcct ttaattatga gccacgaaga atcattgcct aaaataaaac atacatacag
     1141 catgagcctc gaaggaggca gtaaaatgaa acagaacact tttgattata gtccctttac
     1201 agagtcagga aacaaacttt ccttgtgcaa tgttctgtaa atgtggtgat tttaa
//
LOCUS       KCVB01000662            1486 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31271pri ultra-conserved element locus
            uce-180, sequence.
ACCESSION   KCVB01000662
VERSION     KCVB01000662.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31271pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1486
                     /note="ultra-conserved element locus uce-180"
BASE COUNT          462 a          272 c          357 g          395 t
ORIGIN      
        1 gatatgattg cggccatcaa aaaggttatt gctttcataa cattgtatat taaaaacaat
       61 ttaatctcat ccttgtaaac agtaattcaa tctctttcaa gatgcatctc atgtgattga
      121 tgggaaaaga ataagtatat catccatatg atgtcactgg gaaacaactg tcttttctca
      181 aaacgctgtc acctggagtc ttttgacaag aggaacaaag ttagaaaggt gagcgttttc
      241 atctcacctt ctgtacttct gttcttgcat tgagaaaaaa atgagtaaaa ggactttaaa
      301 gtaaaaatat taaaaacaag tgctaaaatt catatcatct aagagaacct tgaggtcaaa
      361 agacaccagc aacaggcaac aaatacaaac catatgaggt cacacacaca ccttcaacgt
      421 ggggtttgat ggagtgtaat gattcctgag atgattagaa actggatgtc gaccttagct
      481 gtcctcattt tggccaccac cattgatcac atcatctcct tccactggtg gcattaaaaa
      541 ctctcatgat aagagtttta tcgttgcaac tgtgtcccag tttgaccaga aacaagagag
      601 aagaaaaagg ctgagaggca gagagaaaga gggagattaa gaggagtgga gaaagagaga
      661 gaaagagtga gagagagaga gagagaagct tttaaaagga ttttgtcggt tattgactgg
      721 ttgcactctt cagctaatag ccatttaaag attatgggcc tgctggtgga aatcaaggcc
      781 tccattgttt gccttattgc attctggcag atgggggctg acacaacaac acgcagcatc
      841 cctccgggca cgctcgccga gcgccgaccc tccatcagca gatctcctat tgaactggcc
      901 ctaataactg gccatgaatt attaatccgc gtaccatatt gaagacagag agggaagagg
      961 aaaaaacaag ggaggattgt gggccggttt cagtgagtgt gattggcgta caggtgccag
     1021 tcgaaggaaa aaaggttcgt tcaaaagagt cccagggcgg ccatttctcg gaacggggag
     1081 gacattccga taactggcac gggaagataa aatatatatt cattaaatgt cagattgctg
     1141 atgaaatggg tccgatccat tagtgggtag cggagcagcc ggttaatgtt tcccggttgc
     1201 tcttgggttg ggtggctttt agtgttgctc tgggttgctg ctggaaacag gatcacttga
     1261 tctgaaagaa gaaaaaaagt tttcaagagt aagagcaggt gttgttcaaa ataaaaggga
     1321 aacgctttgg acatctgagt caaatgcacc tgttggtgaa acccaataac tgttttctgt
     1381 gtttcaagtt gagcgaaccc gatcactgat ttgcagttta taagacttct ttcctccggt
     1441 caggtgattt atttgcaggg attaattact agtttggcta gactca
//
LOCUS       KCVB01000663            1156 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31316pri ultra-conserved element locus
            uce-239, sequence.
ACCESSION   KCVB01000663
VERSION     KCVB01000663.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31316pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1156
                     /note="ultra-conserved element locus uce-239"
BASE COUNT          333 a          283 c          252 g          288 t
ORIGIN      
        1 agtaacaagg ctaatgcacc ttgctagcca ataacgataa ctgaacaaag ccggtaaccg
       61 gtgtggaaag cctgatagct tgccggtgag gagctttgct acaccaaata atgtcaactc
      121 atttatctca aattaataat gtcaatatat tcgaatgagt gctacattca aagcatacat
      181 gcaagcagaa tctggagtgt agagttaact ctcaaaaatc gaactgacat ttccgttagc
      241 tttgacaacc ggcttgataa gctaatgtta gcatacttag ctagctaatt gtcccctccc
      301 ctcccagctc cctccaaacg aaaccatcat tcccgcatcc aacctatatt atcaatcact
      361 aattaaacaa cagcatgtgt tatatgtcat agcggagagt gaaatgcata aaaatcacaa
      421 gtctatgtta cccatgtcaa aggatactga ggtggtggta caacgccggg ctgaggagct
      481 gttggggctc ccggcggcag agacacggag gtcagggtag cccgcagttg gctcgacgga
      541 gaggggccac ttccaggtcc gctaactgcg acagcggcgg gtttggggac cactttcggt
      601 ggcaaactca tcgcaaaaaa atcaacaaat aaaaacaata ccttaataaa tactgataca
      661 aacaccgcct agcttgcatg catagagcaa gcgtagcagt ttcttgaagc aggccccgaa
      721 tccctttaaa gaactgggat aacgaagctc gaatagcaac acaaatgtct tttttttcat
      781 ggaaagtcgc tgttcggatc agcggtgcta ttcatgggga cggtgggagg tttagcctcc
      841 ctcgcccgtc cggaacctct gcggatccgg ctcggttcgt ttcttacaaa ataaataatc
      901 gccacgatct tcaacctccc tcggcgatct accttttctt ctcgacaagt atgttttcta
      961 tcattttttt ctcacagaga tgagagactt cgccaacatg gcgttgcggc cgcttccagc
     1021 agtccgggca ggacagatga ttcggcaatt ctcggccagt tacggatttg ctcggctaat
     1081 taactaacct actatcaaac acagatacaa tgcatggctc atgttcccat gagtctgaaa
     1141 aaacgatttt agtatc
//
LOCUS       KCVB01000664            1846 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31383pri ultra-conserved element locus
            uce-650, sequence.
ACCESSION   KCVB01000664
VERSION     KCVB01000664.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31383pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1846
                     /note="ultra-conserved element locus uce-650"
BASE COUNT          547 a          442 c          397 g          460 t
ORIGIN      
        1 gagggacgtc agcgagcagc cagatgtccc tggctgtgac agtcccactc atcactggct
       61 cccagactga gtgtggggag gcaggacact tggcatactg caccagagac agcaggcagc
      121 agtgagtaat gatgcaaacc acacagctct ctcatcagca aattatgcat acatgcagga
      181 tgtatggtca gcatcagagt ataccgcatg ttggcaaatt caaccaatct gaatataacc
      241 agcaaaaaaa caaatctttt gtactaaagc aattttcgaa acttaaaaag attttgtgcg
      301 ttaaaaaact ctctggaaat cagcaaataa tccacttctc ctgtagtgtg tcacagaggg
      361 agtgagagag agaggcctta atatgtttcc atgttagagt tcccgtgagg ccatgcggtg
      421 ataggccagg gctgtaaaaa caaacagcag agaggttgtg tagggctgac tgtaattaaa
      481 gagcaggacc gtaatctgct ctagcctctt tgattgccca gctccttcga ctgccccggc
      541 gacgctgctt caaaaagccc cactacaact cccctgccac tccggcccct cctcttcact
      601 acggggtcac aacccacatt gccacccaat ccggagggga tcaaagcaca tctcagggac
      661 ctgaattaac atggaatacc acaggcacag ccctataaat atcagcccct tcaaaagaag
      721 aggaacagag ggagtggagg agcgctataa aacaggcctc agccaagtca tcccaagtgt
      781 ttatcttgga cacatttcta tctcagcggc caataaaaca attccactta actgtaagtg
      841 gcccctaaag agaatctatt cctggtccta aaagggctgt gcgattgctt ccatatggtg
      901 ggacagcatg gggacaagca cgatgagctc agacccaggt ccatttggct ggtacctcag
      961 cactgtccat ccacagctgt gatattacat cctaacatct gggttattgc ccgctatgcc
     1021 tgggaattat cagcgttgcc gatcttaggc atcccacact ggcaacatgc ctcaaacagc
     1081 agagcccccc tcccgcttcg cccatacgcc cccctcctgt ttgaaacact atcacacagc
     1141 agagttttac ttaggcactt gccattccct gtctctgtgg tggtaacaca gagggagaaa
     1201 acgatgttct gaaagagggg gagtggagct gtaagagctg ggtattcagc acttccagga
     1261 taggtgttaa atgcaataag atattgtacg tttgtcaaaa atgaaatggg aaaaaaatgg
     1321 cattgttaca ttgttaaatt agcaccgtga tttaggcagc agaacattgt gaagagtccc
     1381 atcacacatt tctgtctccc tttgagtatc tttaaccatc tgtagtggat aacctgcaat
     1441 gtgtatgaaa atggcttcat tcctctgtca tctaatttca aattgagatt gtctactgga
     1501 ccatcaagag actgaacagt ggcagatgta gtgactacat aggtgtgaat ttaaacccac
     1561 tcgctgcagt gtgagtccca aacagcaacc ctcctatttc actctgaatc taattacagc
     1621 tgcaatatat tgaaagccac ttgagaagag atggaacttt gtttactaca aaaccaaatt
     1681 ctgctttata acactcatcg gagggctctt tcatgacaac atattcagtc aatattatta
     1741 ttttggattt ggtccgaatg taatattgta ccatgctttt tttacagcaa acgataccac
     1801 tgaaaataat atgcattgca aaaagggttc actgtaaacc acaaaa
//
LOCUS       KCVB01000665            1419 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31426pri ultra-conserved element locus
            uce-345, sequence.
ACCESSION   KCVB01000665
VERSION     KCVB01000665.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31426pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1419
                     /note="ultra-conserved element locus uce-345"
BASE COUNT          418 a          244 c          298 g          459 t
ORIGIN      
        1 aacacacaaa tggcaccctg tggtgtgttg ggttgttttc ccagtaaaaa cccagaataa
       61 tgctgcttag agaagttgtt gcatctgcta gtgtgggcct gttgtctgtt tgtcttggcg
      121 ttgtgtgtgc atcggtcagt ttaaaggata aaaggagtga gaattatcta cacggggggt
      181 gaaaagaaaa tggatacatt gcaataagtg gttgttaatc agcaatctgt cactttttta
      241 aatagttaat gaaaaaaatg tttgttggca gggctgtatc tctcctattt ctggtatata
      301 ttgtcagatt gtttcacttt agtatttttc ctctcagggt aaaccgggac atacaggtac
      361 atgtgtagaa ctgaacttca catcttcagt caactactca gcctgtcatt tatcaacttg
      421 cagtgtacac atgctccaca cagacaaaca tacagctatt tgatcaatga ttagcctgaa
      481 aagtaatatg ttggaagtca gcaagatttg tccaatctct tttgtgtttt tcaacctaat
      541 ctgtaaaatc ccagcctgca gggaggaatg ttgctaatga gggataaaag cttaacatac
      601 gtttgcacaa tggactggtc aagcagcggt tgctaggcta ctgtggccag aagaatggcg
      661 aggactcgga gggtctcaaa gccctcgttt tgttttgttc cgttcatatt taatctccca
      721 gagagcagcg gtactctttt attgccacaa actgaggggg tttcccttct taattcgtta
      781 tttttctcct tacttacctt ccctccattg ttcctttctg ctccctcttg tttccttatt
      841 atttttcatg tctatttttt gaagacaaca caactttcaa actttaaagg ataatgctgg
      901 agttactttt tcttttatta ttaaccaaca aacttcttga acacatcaaa accaagtaag
      961 aagttatcct gcttgtgcaa gtgttgtcta tgtttaccaa tactgttgca ttggcatatt
     1021 cattcattaa aataaatatg ggcagtatat ttagttgagg acactagctt tgtgcaaggt
     1081 agagattaga gttcagagtt tttcctagat ttattgagac aagcggcaaa ggctgaagaa
     1141 tgtgtgcaac tattctggtt tatataattt gtgtgtgcac ctttagagtg cggtgcgtgt
     1201 aagtggaaaa aaagtatcac aatttcactt tttgcttagc ttgaggacag acaaagagag
     1261 aagacagaaa aggacaaaag ggcgcagaga ctgatgggaa aagtaaagac aagaatgatg
     1321 aaggataaga tataaactga tacaacaggt gttttacttg acatagaagc cattgaagaa
     1381 aatgctgaat tttgtcagca atatttaaaa aattctaat
//
LOCUS       KCVB01000666            1677 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31468pri ultra-conserved element locus
            uce-794, sequence.
ACCESSION   KCVB01000666
VERSION     KCVB01000666.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31468pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1677
                     /note="ultra-conserved element locus uce-794"
BASE COUNT          481 a          367 c          342 g          487 t
ORIGIN      
        1 gtgcatctgg ctcagggagg tatggggttg gccaaatgga gaggccacat ggcggctcca
       61 gatgttttgc ttttgtatat gcacagctca tcaaaaacaa caagcactgc caccctagag
      121 tctctgccat ctgggagtca tcagacaaca gcttctcata ctccctggtg aattattgga
      181 gggctctttt gaaaaaaatg gagttatttt cagtcaaccc cctaatgcta cattcagcaa
      241 ctattaaaac aggagcaccc agtctaccat tgttatattt tgggcagcag gctgctaatt
      301 taaagacagt tattcagctg gagggccagg attcaaaaag catccgcccc gctgaagtgt
      361 ccttcagcaa atcactgaca gctcgggaag atgttttgta gctgctatgt gtctgacctg
      421 cctgcgtaaa atagcatgaa gcaaattgac ttaaccctta taaaaaaaga ttaacaatgc
      481 tgttccaaca ctaacttgtc tttttgtttt tattacttct caacacaaat taattgaagt
      541 aataagagaa ctgttttaga gctgaagttc actttttgca caaagttttt gaatatctgt
      601 caggtcacat tattctgagc gctgtgtact gtgttccctg caggctcttt gtgtccagcc
      661 ccatgtgcac atcacaccca tccccagatc cctgccactc tcagtaaaat cattatttag
      721 tccttttgta agcaagcagt gtcccagatc acaaattaat ctttcatatg tttccaagat
      781 ctagccttct gtaatcccat ctgtatgaga cacaacaaag cacagccaga gagcgcccat
      841 gaattattca ttcatttctc atgaaaggct ttcgttatgt ctgtcatgtt tcactcttgc
      901 aaggaaaatc cattgatcct tgccttttct ctgacaacaa gaacaaacaa gcgacatcat
      961 cacatggctg tgctgtacat tttctcacac tccgacaaat agatctgaca aaaacgcact
     1021 ggcaataaag caatgcataa tatgcatatg tgcataatgt gcacacacat tacaaatgct
     1081 cgctcacaca tactgtgtgc acactacatt aaacacatct tcatgctctt aactaatagc
     1141 tgcaagtgca acctcaggga aaatgtttga actagaacac agtgaggcac aatggattaa
     1201 gtcactcgtg ccttgctagt aatcatttat ctccagtagt attataatat aatgggcatg
     1261 tcttcttttt gacaagttgc cttcataatt cagtgcaaca gaatagagaa aacagcattg
     1321 ttaacaagaa agggctttaa atcacaagca cggagtacat ttcctgtaaa atattaatgt
     1381 ggaaagtgtg tttgtgtaca aggaacaaag ggcagagtgt tgtacatcaa tgggctgaca
     1441 gtgctgcagg ctccacagac caggctctgc ttactctagc tatggcacac tgtgtatgta
     1501 tagggggttg gtgcatgggg gaaagagaga gggtcattta taatatatag gcctgctgga
     1561 gcatgaggtg ttgggtctgc ttaggttagt gtgcatagct ctgcgtggct ggtccacacc
     1621 tcgctctgag cacttgcctt atttacttta aaagctttcc tctggagtct tgtttgg
//
LOCUS       KCVB01000667            1207 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31511pri ultra-conserved element locus
            uce-468, sequence.
ACCESSION   KCVB01000667
VERSION     KCVB01000667.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31511pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1207
                     /note="ultra-conserved element locus uce-468"
BASE COUNT          363 a          229 c          264 g          351 t
ORIGIN      
        1 atgcctggtc aggtgttctg agcaacaaca acaaaaaaaa cctcaagcaa acagacaatc
       61 cacaagtggg accagtttat ccaaaggtaa aagagcaagt caaggcaaac agtttgtttt
      121 cccttcaaac ctcgcctcgt gttcgaacac gagaggacaa caataggctc tgagtgagta
      181 tctcaaatca cagttttatg gagggtgaat gatggcacgg agggtaatgc tctgtgaggc
      241 ctatcagctt tgtttttatg tcgtatacac caggtagact cagtcagtac taattactct
      301 aaatgcaaac atcaggtcag ggtgaaatgg tcagagcaat aacacacagc atttccttgt
      361 tttggcagtt ttctgagcag agtcattagc aaccatcatg gaaggaggaa atgtggaaat
      421 gacagcgtta tgttgaggta atctggatat gtctgtcccc cctccctgac cctggcctga
      481 cccaggacct ctcacaggat tgctgccctt tgtgtcatgc ttctccatgc tgactttgga
      541 gtggagattg caggtacaat gtggattgtt ctcagtgtgt tttcaaagat cagaataaca
      601 atggcatttg ctcagctcac tcactcacac tcattgatca cttgtcccct ctttctctgc
      661 ctgcctgatc acatcaacta tgcttctaac catttttgac agccttttca caccaaagaa
      721 aactaaatca tgtacttttt tacattataa tgttaaaacc ttcaaatatt tcctgtaatg
      781 tagcatatgt tttcatgggt actcgatcac taattatgtg gattttttca atatgagcaa
      841 tgaatctgca cacataacat ttaaatggaa gtactaatgt atgtagaaaa aaaaagtatg
      901 taaaaaaatg ttgtatgaag acttttgtgg ctctggaggg agctgcatga aatctcatta
      961 gttgcctcaa gtgatgtcac ttgagtcagt gttacttggt tactgcatgt ttgaaaatgg
     1021 aaaaaaaata aaataaaaat gcattgaatt taaaatcaat gttacgcaaa agaagcataa
     1081 agttaaattg gtggagtact tttttaagca gagtaacatc tatgggcatc ttactgggag
     1141 gtgaagcgct ggtctgggga tgatgtggcg agctgtgtga cagcagaggg ttgatgccgt
     1201 ggggctg
//
LOCUS       KCVB01000668            1516 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31549pri ultra-conserved element locus
            uce-1332, sequence.
ACCESSION   KCVB01000668
VERSION     KCVB01000668.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31549pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1516
                     /note="ultra-conserved element locus uce-1332"
BASE COUNT          360 a          414 c          288 g          454 t
ORIGIN      
        1 tacatgaact gtaacagctg ctcagagcag atacaggaag cagggtctcc cctcagtcgg
       61 tccatcactc cacagggcac ttaaagtcca gcatgtgttt ggtcctgctg tttgagaatg
      121 tcgaacagag aaacccactt cttttagagt atgtagactc acatgaggat catgtcacag
      181 aagcagcttt gacctgtacg attacagtag ataacagtga gagataatct agatagactg
      241 atgcatcaaa gtggcatgaa agctcacctg tttttctcag ccacccatcg tgtctccagg
      301 cggtgaaagc actcctgtca gttttatacc accgcaggca cactggtctc ccctcctctc
      361 tcctcttccc ccctcgcctg ttagacattg actgctttgc actgctctat ctctgcactt
      421 gaccaatcca ctctatcagc tccgccactc taaactccac agatttgcac cgtgcaaccg
      481 attccatttt tatgatactc tcactcacgt gccttctccc cgcgctgccg ccaaaccagt
      541 cacctgtagg tttagaagta ctcataacca tttcctaatc ccctgccctc ctcgcctcat
      601 tctcatcctt cttgtcgtct ctctctccta ttcccgttat tttcacccct tgcttcaact
      661 gagtttcact cctgcccact tttggtccct tctcttcctc taatacatcc tctgcctcta
      721 cccaaccatt accaattttc tttgacacac tatttgctat tagcccttag tcttcctcct
      781 ccccttcctc ttatccctcc actctccatc cctgtctctg cctatcctcc ttatatatcc
      841 ccagccttcg ctttgccttc tccccatctc cagcagcatg aatatattat tatctacaca
      901 atggagccct gcttattgtt tatgaattac cagccttgcg gccaaacaaa gcctattttc
      961 ataagaaacc tccatttcat ttcagctcca attagattgg gggctaaaga ataggagtcc
     1021 tcaggcctca gcccctaagg atggcaggtc cattcagctt cattcaatgg ggccttattg
     1081 ctttcccata caaaagagca cgtggatggg ctttcagact gaaacaccaa ccaaaaatga
     1141 ctgggagaca gtactataca ataacaaatc tttaaagaaa ggacactgag tgcttatcct
     1201 cccatccatc aattcatccc tcactcttcg ctttcttctt ctcctctgtt cgtatattgt
     1261 aagacctgga cattaatgac agacagttga aagagaaagg gagtgaaaga aagagaagaa
     1321 gaaagaggca gagcagctca gtttttcaca atacatgtca agtctaaatg atcactggct
     1381 gctgaataga cagatggaag ggaaaagaag tgagtcgtgt gtatgtatgt gtgtgtgtgt
     1441 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     1501 gtgtgtgtgt gtgtgt
//
LOCUS       KCVB01000669            1110 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31589pri ultra-conserved element locus
            uce-495, sequence.
ACCESSION   KCVB01000669
VERSION     KCVB01000669.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31589pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1110
                     /note="ultra-conserved element locus uce-495"
BASE COUNT          330 a          242 c          258 g          280 t
ORIGIN      
        1 aagcctccag atatgaagga acacaaatca ctggtcctta ccagatggca gaaaaacggc
       61 acaggataat tgaaaatcta gaagtggatc aatatttaga agcccaaggt tacacgaagt
      121 atgacaatac tattaacatg gaaatatgat gcattacatc cagaggcaaa ttggcttctg
      181 ttatttgatg gtgaatgagt actgaactga aatgcgtcga gtgctgactt ctcaataaca
      241 aagacagcat actagcttta aactctcaca ggttaactac tccaatacac ctttgatcaa
      301 atatgtactg gtgcatgctc atgggcaaag ccacccccat atgtgcatac ttgcacacat
      361 gcagtggtgt gtggatcagc tttggaggtc ccctttcaga cagaatgtac catgaagggt
      421 cccaccatat cacagactac ctctggccca ccccaccctt ctctagcatt tacctctggc
      481 ctgttaaaaa tgatatcagc tcatggaggg caagcattag catttgcatt ttattggaag
      541 cacagagatt tagagcagag gatatatcag ctctgagcag acagaattag ttaacaatgc
      601 attacctgcc ttttatggca gctataactc tttcccctgg tggggagagg agatgaaggg
      661 aggagtgggg ggggtgaagg ggaaacacag ccagcagagg gttggcggtg agagccctgg
      721 cgcagtggag ggggcgttta gaacgaagaa gctttgtctg tggtgcttta ctgcacatta
      781 agtgtgatag ccacccaaat tagagcatca catcgcaatc accttcacac agatcagtgc
      841 actgtgtgga ggcatgatgc gccagtcatc actgcagacc tttttatctg gttgcaacaa
      901 ggaaatggag acacagctgt tgggcagctg tgcctacatg accacagcat gagaaaggcc
      961 acatcaaatt acatctgctc ctgtgatact catacttaca acactcagag agatttttca
     1021 cagtttctct gagacactaa ccgaagacta taaacgtttt ttttccttca taaacaaggt
     1081 aacagtgcat tgcgccatgt gtttgtgtta
//
LOCUS       KCVB01000670            1391 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31631pri ultra-conserved element locus
            uce-807, sequence.
ACCESSION   KCVB01000670
VERSION     KCVB01000670.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31631pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1391
                     /note="ultra-conserved element locus uce-807"
BASE COUNT          425 a          300 c          239 g          427 t
ORIGIN      
        1 tgtatgatca tcagcttcct acatacatat ttcttgaagg gttgtttatg tgcagtatga
       61 ggcacgcggt gcataccgac gaaccttctc cattttacct ttcgggcaca aaaccagagc
      121 cgttaccgcc gctggtgtca catgcggcag tctgccctca tgtccgggat gtggctaaat
      181 ttgccgcagg gaaccagaaa gaatttgaca ggaagatctg acccttcctt ttaatgacag
      241 tagaattccc ttcccaattt tactcaacac gcactgtaaa tgaaagcatt tttaatagaa
      301 aagaacacat acatgataca caattcagga caaatgcgtt agaatcgtag ggggttaatc
      361 tgcagatgcc tttccgaggc acagtaacat gagggcagac tgcataaata ctagaggaca
      421 aaatgtttgc ggaccatctt aacttctaac ctgcaggtca gtttaagtgt ttatatttgc
      481 agctcacttc gaatagacaa acatctgtga agagatgcat cttaaaacaa catgcgttaa
      541 aacttaactg aaatcagaca aaacgtcctc cccgtgctcc tccagcttta ttgatagttt
      601 aaaattacat ttctattttc taggacttca gttgcacttt ccttccgact taaaaactga
      661 gtacaagcaa atggtattta tacagtagtc tactaagtga tattgtacaa aaataacatt
      721 ttgatttctg tgaaaacatt tttcattccc aaataactcc taattctgct gttctgacaa
      781 ctgttcttga tgttaaattt tgccaaggta aataaaaaaa aattccaaag ccattttaaa
      841 ggaaaagtct accttgggct accaagtgat atttttaatt gtaaacagat ttctataaaa
      901 gaacatgttt ctttcaatac ctccaaaaat ttttatatct tagcaatata acatgtagtt
      961 tgttatcgcc cgcttagctg cttgtagggc tgaaatgcat catgttatga actgtttcca
     1021 tatcatactc tcttggccaa ctcatatccc actaaaacaa accaaaataa ccatacatgg
     1081 aagttcggtt tccatttact cccttcttct atctcatgtt actgaggctc tctcttccac
     1141 aagctcagag gtcctataga ttcccatatg tgtagtccca tttattcatc tctgatcacc
     1201 agtggcttgg aaaggggggc tctttaacaa agcattatac ataacaccgc taccaaacta
     1261 aaacattttt gtattgaatg cagaaagatt tttttttcac ccctttcatt gtattacatg
     1321 tcgagaggct cactggcctg ggactagcct ctgtcagcca aactttggcc agtgaagagg
     1381 attcagagaa a
//
LOCUS       KCVB01000671            1298 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31709pri ultra-conserved element locus
            uce-769, sequence.
ACCESSION   KCVB01000671
VERSION     KCVB01000671.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31709pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1298
                     /note="ultra-conserved element locus uce-769"
BASE COUNT          373 a          266 c          284 g          375 t
ORIGIN      
        1 gtgtgtgtgt gtgtgcatcc tagtaacaca gtttccaaca gttactctcc cgccctcctt
       61 ttcctctttt atcagtctgt gttctccaat cactattccc acttgtgctt ctctgcaaac
      121 atgctgttct atgcctcgtt atttccttct cctcaaagag acaaggtcaa agagtcctgt
      181 atagtacttc aaactcaatt caagtatgtt gtctgaatgt ttcttttcaa ccccttcgtc
      241 aaacattgcc tcattaatgc catttccctc ctctatgctc ctacttttgc agccaaatgg
      301 acgaatttta tggtgttttt atctgtgtca gcccgcatgt acacacagga cacacttaca
      361 gcttatcagt gtgcatggaa actgctcttt ttctccatat ctatttgcat aagactagag
      421 agctacatgg ctgttaccaa attattctta cacttttatt ttctttttcc actcatccaa
      481 aagaggagga gaatagggga aacagtaact acactgttag tggtgttaac atccaacaag
      541 ctcctctgct cccccctgct tccctctctc cctccatcct ccctctcctt ccctcccctg
      601 ttaccctggc ctatcagtgc ggagcagtgg tctgtgaaga aattcccaat atctaaattt
      661 acacactgta tcaatcttgt ttaatagact ggaaagctta tagccgccgt ggcaagccgg
      721 cagagagggc agcggtgata aattgtcggt gtgcaggcga cagccgcccg tgatgtataa
      781 tgaaatattt atgatttatc ccagctaata aactgaaatg aagtgcgtga tgtatgggaa
      841 cagatgggcc ctctattgat gctgaagcgg agaggcaaga gggggaagag gagacaggaa
      901 gataggtgga aagagatcga aacaggagat gtgatgagag atgctccaga ggtagtgaga
      961 aaaaatggag ggcaaggata aaagtaaaaa agtaaaaaat aaataaataa ataaaagaat
     1021 agttgtgtgt gtttggatgg cactggagca ggagacgctg aaaaagtaga aaaaatgcaa
     1081 gatggataga ggagcacagg gtaagagtag agaacaaatg agaataaatg atggagtaga
     1141 ggacagacgg acgatggtgg taaaaacaga ccaccacctt cacaataaaa cacttctcct
     1201 aaaatgcttc atcatgttga ttccaggtga gacggggcca gttgtttttt ttgttgttgt
     1261 tgttgttttt tttattgatt ttatattcta ggtgtaga
//
LOCUS       KCVB01000672            1819 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31753pri ultra-conserved element locus
            uce-1033, sequence.
ACCESSION   KCVB01000672
VERSION     KCVB01000672.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31753pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1819
                     /note="ultra-conserved element locus uce-1033"
BASE COUNT          540 a          366 c          371 g          542 t
ORIGIN      
        1 tgtagcaaca tgttctggtg tctgacaagt ggtattttgt gcaagacaac gacctaacct
       61 gctgccctgg aggtctcttt aggcacaaga ttccaggctt ttttccaaat atctttaatg
      121 taaacatcag tgcgatacgc ccaaatacag taattttcct gcccagggca aaaaccactc
      181 atccaccagt ttttcctgag catgtaaata tgatttattc cccctggatt tgttgttttt
      241 ggtgtgtttg tgtcaggtca gctaaccctg aaaccttgct gcaaaaaagg ctaaaaataa
      301 tgcagcattt tatatttcct ctcattgcaa agtttatgtt aaagtcatgg ttcagcagcg
      361 tactctcttc agtcagtggt ccctttaaag actattaaat ctgtctgtct agacattgtc
      421 ctgaggcaga aatatatttt gaaggtcaga cttgaaaaat tgttcatatc ctgtcttttt
      481 gtaatgttaa acagagaagc tttagatcac tggtaatgga gttctgtcat gtatagaatg
      541 atgaatgatt tatctagtct aaagtgattt tcaattaaaa acatgatata atttgagtac
      601 atacagagcg ccatccttgc ttatgcaatg tctatcaact acctcaaaag cgaaacagga
      661 caaaatcggt ggtttagcat gaaggttggg aatacatttg ttgaatatcc gccatttttg
      721 agtttataca gtctgttgtt catagaggat aaaatcagcc atcactctat ggccacagag
      781 caatcagaca cagccctcca gtgtcttcag ctgactcttc tggccctgtc aactcctcct
      841 tcaatctggg agtctgagca gtcgcgtgga ggttgacatg attgccttta agcagcccct
      901 cttagccagt gacacacagc tgcagccagg cacctcttct gacagtaaga gcaccatgat
      961 ggattgcctt gccataactg ctgatcaaag aaggtcaaag ttcagatgtg cgtctggctg
     1021 aacgaggcat gtgcaatcac cagagatggc ttttcctcaa acatcccggc gtaaggctga
     1081 atttttaatt cgctggtggc agcctgattg agctacagaa taattatgcc atttattatt
     1141 gtaataaaga aagataaaaa agcatatgtc aacaatattt aagtgtatcg atctaacggg
     1201 gtcccactga gcctcaggat cttaaaaaag tataagcttc atactagaca ccatcccaat
     1261 ataaagtaaa tatgtcttac tctttgacca gtgttcatac gcacattaat acacataaaa
     1321 tgccatcttt gggctgcaga agcgctctgc tgaaaagagc agacatttaa aagtttctga
     1381 catgtgtcgc actaccattg agacactgcc actgatgtag gtggagctga cagcgtccca
     1441 gccattgaag atggctctgg gacataccaa gtttggtatg tgtcagctct cattgttgtc
     1501 aggatacatc ctaagtgaag tcggaatatg taaacagcat cattaaaagg gggattcata
     1561 tgaaacattt tagtctggag tgacaggata atgtttttga tgtttgctct cgctctcctc
     1621 aaaacactca agtgggtgaa attatccaga tactataaaa catttggaga acggattaca
     1681 ttatccagtg catttatagt gtcatttata gtgtattttt tcttcaaata tgtcagtgaa
     1741 agagtaaggg ccaggttatg cacaaaagaa cagtcagttt ctgaaagtta caggaaagtt
     1801 ggacacagct cgccaaatc
//
LOCUS       KCVB01000673            1544 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31796pri ultra-conserved element locus
            uce-1076, sequence.
ACCESSION   KCVB01000673
VERSION     KCVB01000673.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31796pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1544
                     /note="ultra-conserved element locus uce-1076"
BASE COUNT          489 a          340 c          305 g          410 t
ORIGIN      
        1 tccagctcag agacttctac aagagccacc gaacccccaa ccgacatgag tgtgaggcac
       61 tgggccagga gttgggactg cctcaccgtg tggtgcaggt aaattttctc aagtattcat
      121 cagtaattaa ataataaaat gcagtttgat ctgcaattat taagtattaa aaggatcatt
      181 tcaatgaact cttttaagac taatatgtat ttatacacat ttttttggca ggtgtggttt
      241 caaaatgcca gagccaagga gaagagggcc aggagcctga gctctgactc tgcggagaga
      301 gagcaggctg agttgtccgc cggggcaggg gagagagaca gagcttagag aggcagacta
      361 tggtcccttt gcttccttta actacattct ccctaaaacc ccttcagcac ctgctgtcaa
      421 cctctaaaca tataccctgt ccactttgct gccaaacctg taacctgaac accccaaaga
      481 tgcagagcca cagtggccca aagacaaact ctctcaactg gaagtctgag agagcccaaa
      541 ccaagtcctc tttaccacag ctttcctctt ttcatattgt tcttaaactc tccttcctgg
      601 tctgtacttt cttgttgaaa agagctcctc tcccttttcc atcagacacc ttactacaac
      661 caaacaccac cttgataaaa ccaaccagat gccacagagg ccaaagagca ttctccaagt
      721 atgtgacctc actgcccagc tagacctgca gtctacctct cagtctgcat gacagactta
      781 cagtgggccc tccaccagac agtgctagat gactagctgt gtagcatgta gtgccagtct
      841 gtaagaggct ggaaaagaaa ttgtcaaaga gattttagtg taaatagaga gctccaaaag
      901 ataaacttga agaaaaacaa agcagttaat agtggaagaa gatgatgttg acaaaaaaaa
      961 aaacaaaaac aaaaaaaaca aaaaaaggaa aaatatcctt gaaatagacg gaggtattcg
     1021 catagagctt ttgtaaagac tgactcagat tccaaagctc gtaaccaaaa ttttacatgt
     1081 ctgtggattt agtttcaaca tgtttgtttt caataacaaa ctgcagagct gacgtctgta
     1141 aggtgaatgg gtgaaatctg cacctggcac atgtaagccg agaaccaata gactgatacg
     1201 gtgctagaat gcagcttcac cctccacaag ccaatgatga gtcttctgtt gtacatgtac
     1261 accatctatg ttcctttagt ggataacagc ccaaatagca cagaacgtaa aggccagttt
     1321 gtcctgtccg ggcaaactat tttttatagc actcaaccat tatactgtat atgaaaactt
     1381 taaccaaact caaatttaca ttgtgtgtgt ttagtaatag tttcttaata taaatcttat
     1441 ggattgagat aaactgtctt gcttcgatat aatagtgtca tgattatgaa aagattatga
     1501 cagctttgat atgttcaggc aaggtgactt actgcctttc cgtt
//
LOCUS       KCVB01000674            1518 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31840pri ultra-conserved element locus
            uce-159, sequence.
ACCESSION   KCVB01000674
VERSION     KCVB01000674.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31840pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1518
                     /note="ultra-conserved element locus uce-159"
BASE COUNT          393 a          321 c          363 g          441 t
ORIGIN      
        1 aaataattac ccacgatgat tgcagtttgt ttaagcacta gatagagagg gcttcagagt
       61 tctcatgtta caaaacactg tcttgcgtca cgtggtcttg ttcagctcat cacttgggct
      121 agacttgttg tggtcctttc ggctgatatg tggaaacata aaacaggcat cacacctact
      181 tcccctgtgc aatcagggct tgtccctgtg gtcttcacgg tggttgattt tcaagttcat
      241 tgttttaaat tcccctcctt tctgcacatc atttagttat tagagttgga gggagagagg
      301 gatgtaggaa gagagaagat gggaaggggg agtgtgtttg caagtttttt ttccccctca
      361 actcagccgc tctttcttgc cttcctgttg cattccctta ccctcagtct gcagtcttta
      421 acagaagagg gcttcaagga gaggctggtc gaaggcagac agggatgggc ctgaaagagg
      481 cgtattgtga ggtcacattc ttcccaaatg caggcaggag ccaagtcagt agctggcgga
      541 attaccccca tgaatcatgc ccattgtgat gcctacccag gatgagaaca tggaaaggaa
      601 ttaggtatgg cagagcctta tcagcttccc attcggaact ggtctgccac tcggtgagag
      661 ctgccggaca gcctttccca tcctcgctca tgtagagtgt ccgcgaatat ggaattcctc
      721 cactgccatg tgttgttact taagggtgtg ctgcagccaa gttgctgcgc tcttttccat
      781 ccacgcccgt cctcctgtct gtgcacttcc ttccctccca tttacccaga gatctttgtg
      841 gtaggtcctg gagttcaggc aatattaggt tgcacctctt aaaacatgtc aggctttgtg
      901 gtgaaaatta aagaatggaa aaatgatgaa atgtaacatt ttgctaaggg ctttttcaca
      961 tttaagcaaa agggagagaa gatgagacgg atggaaacac atttcagaac ttttatcgta
     1021 ctgggtttat tcttcatgta cagtcccatt ttctttgctc tctgcagagt ccatgtgaag
     1081 tggtatgcct tgggtggcat ctttggtttt aagatcgacc agttgtctcg ccagtaaaga
     1141 gaggaagacg tgagaaacaa aatattttta cagcccaatt ttggggtttt tataaatcat
     1201 tatgtcacct caactttcca tccgaatcca ttcattagtt gctgtttgca aagaccagaa
     1261 atcccactcg actctgctct ttgtcttggg gtgagagatg atacatgata tgacaaatga
     1321 agtgaatttc atggtgaaca aagaaatgtg tttgtcattt ttgtcaagga ggatgaaaga
     1381 gatagacatc gagagtgtaa tgcagtaata gaaaacactc cgcagcgaag gcatgcgttc
     1441 tcactcaaaa ccgaccctct ggtgatttga atgaaaagag ccagcagagg ataaatgtgt
     1501 ctgctccgtg ataaacta
//
LOCUS       KCVB01000675            1699 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31882pri ultra-conserved element locus
            uce-588, sequence.
ACCESSION   KCVB01000675
VERSION     KCVB01000675.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31882pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1699
                     /note="ultra-conserved element locus uce-588"
BASE COUNT          476 a          413 c          320 g          490 t
ORIGIN      
        1 gggcgacaaa gatcaacatg ttggccaaaa gccattttaa acccccgagc aaaatataca
       61 aacactgatg acctatacag aattaccaca tttcaaaaag cagccttatg gggagttaat
      121 gtataatatg ctaaaagttt gtagggacag tcaaacagca ccaacaaaag cagaatctac
      181 agccatacac caaaattgta tgcttcctta tgccaaaaac tttcatatac ttgttgaaga
      241 tgtaacacat gctccaaaat ttctccacag cccagaggtg ttacactttc ttctagaaaa
      301 attgaggaag ttctttatat ctgtacaatg taattttagt gtccttctag gactgaaacg
      361 tgagacagag tccaacaata aatcacatcc ctgtttagag cgggcgtctg gcacgactgg
      421 ccggcttcag ctccccagac caccagcacc tcctccaccg ccgcctcctc ctcctcctcc
      481 tcctctcctc tcatgctctc catccctcat gcctgttaaa ggcttgtcct gcagggcctg
      541 gatgcacttg tgtggtacaa caacagagaa accgcccaaa gctcttaaca gctcttcctc
      601 tccacttcct ccctccctcc atctctactg ctaccaccac cagctcttct ctccctctcc
      661 actctgctct gtctttccca gctgcaccgg cgagctgttc cgtatgaact gctgccagct
      721 gaaagcctgc tactcctgtg agattgctat tactcagtgc aagcagggct atgcgtagcc
      781 aaagggctgc tctccgagct gtagatctcc atgttgtgac ttgtcaacac gggaaaaatc
      841 gttaccaagg ctgaggcact tccattccac acagaggggg tctgattgag accaggcaga
      901 caggcaggta ctgtactgga ggtattagga tgactactgt ttcctgattt tgtgctgcag
      961 aggattctct gcttagagag gccactttga agttattaga gaacaaacat ctgatggaaa
     1021 aaaactacat ttccacagtg aatttcaact caagctattg atattaaatt aagtaatttg
     1081 taattgtgtg tttcccctat attcattcat ttattcattc aaaattataa gcgccactgc
     1141 caaaatttca caggatcatt aatatcctct gccctcatat ttcactgagg gcatggtccc
     1201 tccaaacaca cggagtggag caggggagag aaaggttaag tgcacttcac ctgtccctcc
     1261 tctgctccac agcgacaaca ttatctttaa gataaggtac atgctcggtt acaaacttgt
     1321 gaggtttcga tccatcaatg tgtcgatgga tttgcccttt ttttgttttt ttaaggtaaa
     1381 ccagagaaaa tttagttaat cactattcac tattctctaa gtttgttgga agtattgtaa
     1441 agtaaaacag cactttaaat gctacgatgt tggtcagcaa ccatcatgtg gtaacttaaa
     1501 acaattcagc ctttgtgttc tgattaattt ggcatgcagt taacatgcca gtcataattc
     1561 tgttttaatg aaaactcaac tcctggcatt tgctcctggt gttcttcact gttttgaaag
     1621 aggaaaataa acggactaac aggatcttta taagtgacag attttctttt taaataagtg
     1681 ctgtgcggtg gatcaaata
//
LOCUS       KCVB01000676             987 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 31922pri ultra-conserved element locus
            uce-631, sequence.
ACCESSION   KCVB01000676
VERSION     KCVB01000676.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 987)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="31922pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>987
                     /note="ultra-conserved element locus uce-631"
BASE COUNT          274 a          207 c          217 g          289 t
ORIGIN      
        1 tgaatcctgc aaggatgtaa acaaacttca tcccctgctc tgaattctgt tcagccgaga
       61 acaacaaaag ctgctcccct tcccttatat tttttttcct ccagccaggt gtcatgactg
      121 ggtccttgac ggctcctgtc ttccccggtg ccccggtggg cccccgaata cattacaata
      181 attaagcctt ccgtgattta gcctccttgg agagaagtcc aacaatgtaa ttaaggacaa
      241 gctcagtgta ttaggtatga atattcaaaa ctgtgtcaat taattagccg gtgtatctct
      301 cccgttatgt ctcagtcctt caagcccact caaaaggtca actgtgccga cggagagcat
      361 attttaaaga catcatgtaa ttgcaaggaa atctctttat agtgccactt cgcttgaaaa
      421 agagtgaaca attatacatt cgctgctaaa taaagacagt cgttgttgtg cttgtgtgta
      481 atgtttttgt tttctttgca taactgtttt cctggcgcct gaaaaatgag ttttgaaact
      541 ctgaggccct cagaggccaa cgcgggtaaa agcatcctct ttaaacgccc gggaaagatc
      601 aatattaaat ctgttttcac aaagggggcc gttaaataga caccgtggct gatctgcata
      661 acaaaattaa ttaggtagtc tacggcagtc ttcgcctgca cccaactcct ggacaaataa
      721 ctagttgtaa agcacacctt ccgggcatac ggaacatatg ctggaattat ccttaattga
      781 ctaattacat aaattactca ttaagtttac agcacatcaa ttataaaaga tgtatcaatt
      841 aattttgcat aaattacaag aggcacgctg cacgaagagg gcggccgagg gaatgagggt
      901 cgccaggagg gggtctaggc taaggtggag cgtttatgtg tatgtgtgtg tgtatgtgtg
      961 tgtatgtgtg tgtatgtgtg tgtatgt
//
LOCUS       KCVB01000677            1221 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32000pri ultra-conserved element locus
            uce-689, sequence.
ACCESSION   KCVB01000677
VERSION     KCVB01000677.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32000pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1221
                     /note="ultra-conserved element locus uce-689"
BASE COUNT          332 a          282 c          244 g          363 t
ORIGIN      
        1 tctctatctc tccccgtctc tctttctccc tctgcgcgcc ccctcccatc ttaaaaacgt
       61 acacattgtt acaagaaaaa aggggtgtgt gactgcatgt aagttacatt gttacttgtt
      121 tgtttctttt ctgtctgtgc cccccctttc tcttttctct ctctctccct ccctctctcc
      181 ccgagtccgg atcagtcacg aatccgacac acttgcctgg tgtcactgtc acttgcaaga
      241 tcacacgcaa caactctcgc tttctgcatt ctgcattgca gagcccgaca caaatctatc
      301 aaatatggcc accttatact ctccggagcc cccaccgttg tataaaaaag tattttggag
      361 cagcagcagg cttgtgtgtt tgtgtgtgtg tgtgtgcgtg cgtgtgtgtg tgtgcgcgcg
      421 tgtgtgctgg ggagggattg agagaggggg gttgggaaaa tctgccgcag ccatgcccag
      481 acttacatca gattgtattg cttaaaaatt aactacttgt ggtggggatg aaaacaattc
      541 agaatatgca aactacattc ccccctttat aaaccaccgt atgaaacggc tcagagtgca
      601 aaacccagct ctgatacaga ggtttaaaac aaaacacaag caattttgtg agtgcttact
      661 gtgaaatcac aagcagcagg ctgtgtcaac tggatggcag taaggccatg ggaatcccta
      721 catcccatcc cagccctcag tggctgctcg gggcactttt gttttggcaa aaggaatttt
      781 gatttgcttt tcacattcaa acctcacagg gaaatagggg ggcaatcagg agaaactgtg
      841 gcaacgctat ggatgaaata tgaaaaatga cctgcattgg attgaaacat ctgcctcccc
      901 tatgagcttc tggagcattt tagacataaa ataaagcaat gtgctaaaaa tttgtaccgt
      961 ttatcaatcc atgcactatt gatttcatgc attcagctaa ttttattgca tttattcact
     1021 taaaaaaagc aatagtttag aaaagcaata atcatattcc tcacaatgac aatatatttc
     1081 cttgatgcag aaaatgttgt tttattacca gtaagaagcc tttttaagcc ctgaatcaca
     1141 caaatatcaa cgcatctttg gtcaaaggtg acaagaagct cttcagttct ttaatattct
     1201 ttttatttga gctgaaagtt c
//
LOCUS       KCVB01000678            1575 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32043pri ultra-conserved element locus
            uce-258, sequence.
ACCESSION   KCVB01000678
VERSION     KCVB01000678.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32043pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1575
                     /note="ultra-conserved element locus uce-258"
BASE COUNT          412 a          366 c          340 g          457 t
ORIGIN      
        1 agttatactg tagaatattt gtgcaacagt ataatatgtg taatcgtgca tgtgcagtat
       61 gttgtatgtg tttccacctc gttgtctaaa gacgtacaaa caaatctttg cagcatcgtt
      121 gtatatgtca tgatatctta ttcatcaggt tcatgcatcc tcctcgttcc atacacctct
      181 acacgttcac gaacatcaca gtggggacgg agggaggttt aagttgttgc ctttgtttgt
      241 tgaatgttta tagcttccgt tagcacagtc tcaacgacca ctgaggaacc agtagataca
      301 aaatccgaat ccccctataa aagaggagat gccctctggg cagccatagc tcatagttgg
      361 cctgatttag tggcagccag gaatctgggc ttgaataaag acagagagag cgagggcaga
      421 gtaggagaga gagagagaga gagagaggaa aagatagctc cctgtgtgat aaatagcccc
      481 ggctctctct attgcattag ccgtccgggc cgatagattg cgagcccaat agacccagac
      541 gtatgcgatt ggtttctcat ataaaagttg catattaaat tccttcacag gactgtgccc
      601 cccaagcatg aaggagggaa ggaagaaatg ggagtgaaaa agcagaaagt ggagaaagga
      661 atgggagagc aagaaaaagg ctttttatga gaggcgaaac gaaggcagag agttgggaga
      721 gccggagaag aggggggaaa agataatggc gggaggaaaa gtagcttgaa aagacaaatg
      781 gtgatgtcat gtatttaata ggtccatgga tattgggcga tgagtgcgac cgtacgtcca
      841 tttaatcagt attgcttccc aatgaagatt tctctcttgt tttgcgctca ctcccccctg
      901 cttctgttct cagccgttct ccacatgcta acgacctctc taattgtttc caagctcatt
      961 aagatgctga tgttttaata gtaattaatc cgtcatttgc ctacatgcaa acacgggcca
     1021 ctgaggttta ttcatcgcca tttcaataca ctgataatgc agtagcctag tcaaaggaga
     1081 caatatcctg ctaatttagc tcatcattgt taattggttg ttaagtgcct ttaattacca
     1141 ggcaggcttt gggattttgg tcggcagaaa gaggaggatc gctatgattg tccctgccgc
     1201 cgtaatggat cttgtgtgaa ctttgaaaac tggaataaat gaccaaaggg taaaaagaga
     1261 cacacactga ccctgttctg ttttccaaac gctgatgttt tctctctcat ttcctttctc
     1321 ctcttccttc ctttcctccg tcctcctgtc ccttcctcca tctctgtctc gctccctatc
     1381 tctttctcca cgatattatt aactcattaa gtttttttca ttatttttaa taaaagatga
     1441 gttatggctt tagcctcact gtggcagcac cccccttata ccctcctcct ccctcctctc
     1501 tcccttcccc ttcaaccatt tactcctttt cccctcctcc tctccacccc cctatgttct
     1561 ccctcctcct tggcc
//
LOCUS       KCVB01000679            1456 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32088pri ultra-conserved element locus
            uce-1196, sequence.
ACCESSION   KCVB01000679
VERSION     KCVB01000679.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32088pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1456
                     /note="ultra-conserved element locus uce-1196"
BASE COUNT          495 a          273 c          301 g          387 t
ORIGIN      
        1 aaaatcaaaa ctacacaaat aagaaatctg atttagagtt gaatgtgatt ctgtgtgttg
       61 gttgaatgtg atttgagtat gtggtgaata gtttatctct ggggtctgag ccctaactat
      121 ggaagcagag caccagtgaa acattaatca ccaaagtatt atggctcata tccttggaca
      181 gcacattaat tcaggtggct attaggctgc cttgcagcca accttcgaaa aaggtccaca
      241 ttgaaattct gttgacagtg tatagtgtgc agaaagctaa ctatggtgtt actgcatcca
      301 aacaataagc aaaacaaatt gcagatatga atataaccaa tatgtaatta tatttagtgg
      361 agcgggtgct gcaacaaaaa tgtgaacaga ggacttcagt gggcagtagg tgcaatcact
      421 ctggactggc acaacagtct tccctgtatt ccttgtcaat agagacagac gctctacctg
      481 tagatggaaa ataaaaaaat aaaaaagact gaagaaacac caattagaaa ggctctctct
      541 cctgcactct ctcagaaaag ccaggctccg ctaaaggtca gcctgaaatt tgaatgacag
      601 ggccaaagtc ttttactcat aatttttgtg tttgcaatag atttcggagc aattcacagc
      661 cttaaaggcc ttaagtggaa cagcaattaa ttgtaatgaa taatctgaca aatctggttg
      721 acatatggcc gctaatactc ctcctttcta aagggagcag tggagggatt tgattacctc
      781 aaatgaaatc tgggagcgct gtgccatctg tatgtacagg gagaggcagc gcacgtaaaa
      841 caggacgtgg gtggaaccgg ggtctgctcc ctgctgctcc agttccctca accttaccag
      901 caactgattt gggaataggg cctgtttttt tcctcatctt attgaaccag ctgcctctca
      961 tgaagtaaaa cgcaataatg ctgcgccgtt gccaaaatcg aacacaccga gaaaagtaaa
     1021 caaacatgac agaagtgata agtgttgtgc aaacataaaa acaacaccac cgcgtcgagc
     1081 catctgagcg cagtgaagcc ataactttat taaaagcatt taaaagtatg atgaattatc
     1141 atgccgcgta tagagcttca catctgttca tcgctttatt tgtaaagcta cacaaagtat
     1201 cttgtacagc agctttagga cattaaagat gcatgtaatg agtggcctgc tacacagtga
     1261 taggaaacca tgagaagtca tgggcagtga tagaagtatt tagatcattt actgaagtaa
     1321 aagtagaaat aacactgttt aacaattctc cattacaagt taaagtcctt catcaaaatt
     1381 ttactaaaag gtgcaatatg taaaaatttt agtttaaaac ataaaaaatt aacaaaaatg
     1441 atcaaaagaa tgtgag
//
LOCUS       KCVB01000680            1430 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32191pri ultra-conserved element locus
            uce-220, sequence.
ACCESSION   KCVB01000680
VERSION     KCVB01000680.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32191pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1430
                     /note="ultra-conserved element locus uce-220"
BASE COUNT          448 a          275 c          279 g          428 t
ORIGIN      
        1 tgtgaacaag tggggaggaa tgtgagtgac gtgctcacag taaacatagg aaatgcacct
       61 gtgaggacaa tgtgttgtgg aggaacaaaa aaaaatgcac acaaggcagc tggaaacctt
      121 ttaattagtg ctaattacct tagcagagga acgtcgggag gcttgaaagg atttgtgtca
      181 gcgcgtgaga gatcaaagat tgaggcggtc acgttttgtt ctgctgagtc aaaaactaat
      241 aaggagggtg ataatggggc cctggagaat gacaacccag gtggtggtgt gtttaatcct
      301 cactcgagcc ttgttccagt ttaacaatca gatggttcaa ttttcgtttt tttttttcga
      361 atcaagtttt aaaataacag agattttttg gtaaggcatt ataaaggctg tactaacatg
      421 gggtgacaaa aattcatgaa ctaaatgcta taaaataaaa aaaatcacat taaaattcac
      481 atttaaagat gatagcagct atttctgtat tcattattca agtacagaat gattaaacca
      541 ctgctacggt gtcaggtaag cctgtcagcc agcaagtttc atttttatga ttgtataagc
      601 cagagggtca gcaagctttt cagtcacaac ccatcaactc aaacccgtgt gatggtatct
      661 gcaagttttt tctggaggtg ggtcaaacct agcttaaaag tcggtcccct tcacatccaa
      721 ttcaccgtca cattcttacc tacaggcaac ctctgctgag ccccggtcat taatcaaagc
      781 ggagtgctgt cctgacctca gccccttccc tttgaagtgc tctgtcctct gcagcatgag
      841 gaaatcattt gctgcctgat tcaatgagcc atcagtcgag ccagcagacc taatgacatg
      901 tgagaatatt aacctcctac caccaagagt gctgctgctt ggaaagcagt ctattattca
      961 tccaaggttt tactcaaacc cttttttttt tctttttttt tttttttaca agactgcctc
     1021 tctgttaaac cctccctaaa aaactgtaac catgcagaca cccagtgcta atattatatc
     1081 cactcttaat aagacggata taatgttaga taataatgct agattttatt ttaagacagt
     1141 cctaaagtct aacagtcttg caaaacaaat tcatgttggt aacatatgta tgcagtctgt
     1201 taaaggtaca aactctgatt caaattgtga tacaaatttt gatattgtgt gaataacagt
     1261 gacatcccaa accaaaacac aatggggcct ctaatacgtg taactaatgt tttctgatgc
     1321 tgtaaaatgc ttgtttacat cattaatgta acaaacacag gagtaaaaca agtcctattt
     1381 ccattctaat cagttgagaa agatattagg acattataag gtgattatta
//
LOCUS       KCVB01000681            1416 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32233pri ultra-conserved element locus
            uce-265, sequence.
ACCESSION   KCVB01000681
VERSION     KCVB01000681.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32233pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1416
                     /note="ultra-conserved element locus uce-265"
BASE COUNT          409 a          273 c          295 g          439 t
ORIGIN      
        1 aatatgaaat tgatttggtt gaagggtgtt aaagagccga caggagaatg tcacaaacaa
       61 tgaatggtca cagcttggag gaaaatcaat gacagaggtt gaagccagtc tagagtgcta
      121 cttggcagcg ttacactctt cctggaaaag agctgtcttc tggcctgtct gatcaaaagg
      181 aaagcttgat aggtttaatg aaggaggcta caataaaact gacaaatgaa atatagacat
      241 tgcagcaaag attgtttgac ttcagtgtag gtggcagctg tgcactgttt gaaattcatt
      301 tttaatttgc tgctgacttt ctgagtccat acttcatatg tatatgcggg attgtaatgc
      361 agaatcacag tgactccctg gtagcttgta gggcaacatt tgtgaccttg taaagccttt
      421 atttactatg atgtatacat attatagtgt ttgcagtaaa atttaacatt ttggagatat
      481 gtgacagcat tgcctcaaaa gtcttactgg cctagagatg agaaaaacgt ctgtatttta
      541 cacaagttat gttaatttta ctgtgtaaat gatgatcctt ggaaggagag gaaaaatgaa
      601 ttggcagagt gtgtttcaag tggacaaagt gatgaaaatc ccccctactt tgttgggatt
      661 gcagttgtaa gctttgcacc atatgctact ttcctcttta gctgggatta tgccatcatt
      721 attgctgata aaagctgttt actgccttgg ctccaaatcg atgctcgggg cgctctgaaa
      781 ctggcattcc ctctccagaa gagttcattc catgccagct gctcaggatt caaccccttt
      841 tgtttaagat ttcacagttt ttgtaatgtt tattttctat ttagtttcta ccccccctct
      901 cacacatgag gtttttcctg cattagcata tgtttgcaac aatttgacat tcacatttga
      961 tttgcttaca gcagattgaa ggtgtctgaa acatggttat acaatactct tggagccacg
     1021 gtgctggtta acacagcaga gattgtaaaa tactaataat aagacctgtt ttgaggagat
     1081 gttttcagtt ctaaaatgca gttgtggcgt tgaaaaggct ctgaatttgg ggcaatatcc
     1141 caaaggctcc cttctcacag tgttaccaat caatggcata atcagtcaat gcagattcag
     1201 cccgacagct gtgttaagat aacaactggt tctcatgact atagcggccc cagtgacctt
     1261 gtgtgcctca aattaataat gtaaaatcaa cacaagacaa ccacccccct ggtttccacg
     1321 cagacaatga tgaactacct tttcgccata ccatcaaagg ccactcaggc taagccatgt
     1381 ttcaaggcat agaccataat gtcacttttt tatttt
//
LOCUS       KCVB01000682            1982 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32316pri ultra-conserved element locus
            uce-1212, sequence.
ACCESSION   KCVB01000682
VERSION     KCVB01000682.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32316pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1982
                     /note="ultra-conserved element locus uce-1212"
BASE COUNT          507 a          431 c          464 g          580 t
ORIGIN      
        1 tgtgtagatc ttgactgtaa tagtacttga cacctatttt agtacctttc aagcacaaag
       61 aggctataat ctgccaaagt ttttgactcg ggagaggaag aacaattgtc ttggaacaaa
      121 tggataggag aagggttttg ttttcccaga gataatttga agcgggtcaa aaatgagagc
      181 atcataatat gattgccagg tgaattgaac gaggcccatg ggccgggggc cctctttgtt
      241 tttccagcag cggtggctgg gagtgtttga tggatcctaa ttgaacacat atttattgtg
      301 ctaggttcag ctggagtgtg acgggtatgg tgatgcctca attagaacca ttaacaggat
      361 gattgtgctt gcctttctgg aaactttctt agcgaatgag gagggttttt tcccctgcca
      421 ctatgatcct attttcaaag cacaagtgcc cagcggccat ctgggaaaat cacctcatgc
      481 tttgacgaaa aggatgatgg cggctcgatt cgtcagttgt cgtagaggca taatgcaata
      541 acaatccttt tagcctatga aagaaaaatg ccaggatgtg tgaaacagtc aggagggtga
      601 ggaaattgtg cttttagatg tctgtccatg agctagtgac tgacacaaag ttatcttcac
      661 tcctgtcttc agggcaaaat gtgagcagtc acaggaggca tgctagagtg tcagagccag
      721 aggagaactt atagcttgtt taaggcttag gagagtgttg tgaccgtaga ggcctgtgta
      781 gcagctgata ggtttgagtg tgtctaaccc tgtgatctgt gtgctaacat tggcctactg
      841 gggtaagatc agttcctcaa ctctctgctt ttgtcccctg tgaagggagg cttacccaag
      901 gaccccctgt ttactctggc ctgcacaatg gcgaagtcta aactcccttt gtagggtgga
      961 tttaggctag atttacttca atttgatctg ggagggcccc ttttcatcag cctggaggtc
     1021 taatgcccct aactggctta acctggctcc ccctgtgagc ctaggaccct catgcccaca
     1081 atctctcgcc tctttgccac tccttcttat cccccgcttg ccgcctcacc ctcacgtccc
     1141 ctgagaatcc tcagtatcac tggggtttga ctgtggaaaa gcatacaggg gcatggttgg
     1201 tgacacctga aactcatgtt taacatgtca taagcctgct aggaatcagc ataaagcatc
     1261 agcatgaaag atgccctgtg tctaaacaat gtgtgagcaa gagacattct gacaataaac
     1321 aggtagatgg tatggacttc tccttttagc atttgctttt aaatgtactg tgaaaatgcc
     1381 acaatgcagc ctcgccaaat agatgcttgt ttcttcagta ttcttgaaac tgtttcgagt
     1441 ttaatatcca ctgtagtgcc tttgctcatg aataaaaatg agatgtcagt attaacccca
     1501 ggactcagag gtctcctaga taggagagtg ctctctctgc aagtagaggc tagtctttca
     1561 gaaaacatgt tttcaagata cagttaggaa gtgataaaag tgtgtatttt agcatgcctt
     1621 ttatcacagc tgtcaagcat catcatgcac tctgtatggc actaaagctc atcggtggtg
     1681 tgtgcccctc tagcactgat atcttttttt cctcacaacc ttatcatctc agtttgtagt
     1741 aatccggatg gaccatcctt ttggaacatg tagaacttcc tgttggcttg aactgggctt
     1801 cctggttttc aaatcaaacg gtcaacatga ggaaaagcac agcgccatga tattgtcatt
     1861 tcccaaatat gctgccagtc tttagtcctc atcttttgac acagccaggg gtaggctaac
     1921 cacaaaatgg gctcccgttg ttgctatgtg caatttaaac cctgcctccc gaattctgta
     1981 tt
//
LOCUS       KCVB01000683            2571 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32359pri ultra-conserved element locus
            uce-199, sequence.
ACCESSION   KCVB01000683
VERSION     KCVB01000683.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32359pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2571
                     /note="ultra-conserved element locus uce-199"
BASE COUNT          732 a          495 c          607 g          737 t
ORIGIN      
        1 gaaatgcatt atgagataaa ggcaattact gaaaattaat atattatcag catagtttgg
       61 ctggctgatg aaacggatgc tggaacctaa accatgacag aaagtttacc tcaagtcagc
      121 acagagccag cttatctgct tttcatgttg atgttttgat catgtattgt aagtgtgaca
      181 tgcaggcagt gacagtagtc ttctgacttg gccgccatgt ttgttttgtg taacacagaa
      241 acttctctca ggccttttta aaggtaccta ttcttttatg ttttcttttg actttaagct
      301 ccgacaaatg gtgaatgagg cacaaaacaa agagttcttt gaagaacaaa gtaaacctgc
      361 cacatgcaac tactggctgg agacgaatcc catcgcaaag attgcatgct ttcaagacag
      421 gggagggaaa agcatcccta acaacaaaat gatataaatt accaccgtta tggtctatac
      481 ggttcaattt aatgtgcttg taatgataaa ttattttggt tatgaatcta aagtggacct
      541 gagtgcagtc ggagcagcag gactgttaga acaattgcag ctgtctccca gggctgtggc
      601 cattagtcaa taaattagaa agtaattagt ttctgcttct gtggctctat ttgaagcgct
      661 tcacacaggt gtaatgaaaa tttattggag ctgttgatcc ggaggagaaa agaaaagtag
      721 aggatttgta gcgtgtgatt gtaatgcagg atttccatct tcctctcgcc ttttttgctt
      781 gcagcgacag gtaaagttac ccatgaagac tagcgttgtg agatttggct tagatgttcc
      841 aagctttgtc agcaaggcaa ttgactagca gctataaata tttcacatgt ggatgcttat
      901 aagaagactt cctttttaga attttttgaa ttcatgacgt cagatgattt aactctaaca
      961 caaatgacga ctattggtgt gaagaggtaa atgataatga cttaaatcaa cccctgagca
     1021 ttgatttgct agtgtctgtg ccctctctgc tgtctcattt gcagtcataa atcctgttgt
     1081 tcgtccacag agcaatctca caggtggaat attcacgtac aacatacagc atcacataaa
     1141 gtaaaatagc atacataaaa tttcagagag gtcagagagg tgttttctgt gctgcaattc
     1201 aacaatgtca cacactgtgc tcacacaagc ctatgaggat gacagctagt tccctggact
     1261 gtctgtttca ttctgattct ctgtgctttt tggccagcgg gcatcttatc accctttgtt
     1321 cttcatactg cacgctcatt tttttcagtg tcatttgacg cacaggctgt cttctcactc
     1381 ctacataaaa agagggagaa cagcagactt cagccatcag gtttacctaa ttgaacagct
     1441 tcacatttct ctttcattgt gtgtggatca aggcagacac acaaagcttt ttgttgccag
     1501 acaacatcaa ggctatttgt tttgtctggt ggaatgtaag gtcaacagga tgggaagaac
     1561 agaaattgga aagcgcttgc acaattgtga tgtcaatgaa cggcatggac ggcagacaga
     1621 gggagggagc agcaatgaca gcatatgggt aaaaaagaaa aattagaggt gaaagagaga
     1681 tgagacggag agaaaggagg cagggaggag atgaagaaac aagcacaggc gagaagttgt
     1741 gaaggcagcg agggagaggg agaacgacag aggaggaaca gaaaggacgg agagtggagc
     1801 agagacagga tggacggatg gatgaggaca atgaggcaga tcggtcccag cccagtctat
     1861 ggccaattag aatagaatta gctggacctt ggcaaaggct agggcgcaca gtagggcatg
     1921 aagagaggtg ggggaggggc aaagggggag aatatcccgg tgattaaaac caaatgatta
     1981 gtggtgtgca aggcagaggg ctagagagct agggtcacct gagcctgcag ggagagagaa
     2041 ggtctaatag taatgagacc aaccttccca ctcctcatcg tcaccgtcct ccttttaccc
     2101 tctcaccact ccttaccttc cttttctctc attcagactt aattttttcc cctatacagt
     2161 ttttttccat ctcccactgc tctttattta cacgttctcc ctttttctgt ctatcactgt
     2221 gcttttccat gtctgcgcct cctgccttcc tgtagtaaac cctattacta tctattactg
     2281 tctcttgcct ttggccaaag cgcgcactaa tgccattgtt gtgggagact gttaagcttt
     2341 ttgtgataag caagatttat gagtggtttg tttatctttt ttaatcatgg gtgtagatct
     2401 gcagatgtat ggtaatagat ggattcagct gcaacaaaac tcaacataat actgtcatct
     2461 ctggcagaat ccctatgtgt tgctgcttct gtttggtcag aagaggcagc acatggacag
     2521 agtgcttgct tttgtcgaaa ccaacaattg ttggtggtat gtgatttgtt g
//
LOCUS       KCVB01000684            1010 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32474pri ultra-conserved element locus
            uce-164, sequence.
ACCESSION   KCVB01000684
VERSION     KCVB01000684.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32474pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1010
                     /note="ultra-conserved element locus uce-164"
BASE COUNT          325 a          194 c          236 g          255 t
ORIGIN      
        1 aagcatgcat catgcttgta aatgcatgta atgttagtga tcttggagac gaagcctgca
       61 ggactcactt taagttctcc tggaaaactg tgtaagctaa atgctgataa tgtggaggta
      121 aattcgattc attcctggca tgtgcacata tagcagtaca ctggcagagc gatcccagtc
      181 atgagggatt tatttagcaa atattccagc agcccagcaa agaatctagg agcccattcc
      241 actgtgtttt taacagtccc tgatgccata cgtgcctgga atgaacaata gtgtgtttcc
      301 gcttgtggcc agtagggggc actgacataa tacaggaccc tgccaatctg ttatgcctgg
      361 ctggcccaca gagatgtttg cagtaattta gttccactgc caatatataa ataggaattc
      421 tgctgttagc acaaaagagc gggggggaaa aacataccgc cgaatgaatg tgaattgctc
      481 ccctgctccc atttcaacat caaagtaatc aaggaaaaca agctttttta atcgaaagta
      541 tgcaattaga ataaagcaga actgtcctgg ctgtgcacac tgccagaatg aggagattaa
      601 atgggtaagg agagagggaa agcacaatca gtgtagcagc agctatcgga ctgcagtcag
      661 aacagataat gtaacacctt tcatttctgt cctgtactgt tacgccaaag aatgactgaa
      721 ctgtggaaga ttcaagggga gtgaaaacgc caaaacataa caagcagggg agagacgact
      781 ttgtggaaac agcaactaat aaaaaggcgt ttttgttaga acactaagac acctgagatg
      841 tcgaaagacc tcattttact gtgtgtgcag tgagttacta ctgcacttct actcaaacct
      901 gaagaagaat tactcagaaa aaaggaatgt atgttacaag gaagtgtcaa agagtgtggt
      961 aaatagtctg ttacgatgta gcaccaattt gtttctggag cattaatgca
//
LOCUS       KCVB01000685            1317 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32509pri ultra-conserved element locus
            uce-927, sequence.
ACCESSION   KCVB01000685
VERSION     KCVB01000685.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32509pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1317
                     /note="ultra-conserved element locus uce-927"
BASE COUNT          353 a          283 c          289 g          392 t
ORIGIN      
        1 ataagccaat aaaatgtctg tcagctacag aaacagcttt gtgaggatcc ttcataacat
       61 tttaaagaaa catcagtttg cacatatgtt ttgaattttc tgctttaagt ttagcatgtc
      121 tgcgtttttt atggctcaga acatcaattc tgtcatctgt ttattaggat aaataagttc
      181 actccatcta caaattgtcc tttggaggcg tttaaagtca atcctaaaat caagctgact
      241 aaagagtcct ctatttgctc agataaaaac actatacact cctctctaag ctggcagaaa
      301 ggaggagact accggtggtc tcccaagttt gtacagcagt gcttttcctg ataatgccac
      361 acattcgggt ttctgctttc agtagccttc gcacgagtgc tccagtctca cacggcactt
      421 aagtgtgatt aataaggtgc agtgttgtgt gtatgtgtgt gtgtgtgaag ggggggttga
      481 ggtggaggtg gtggtggtgg tggtggggga gtttatgtca agataaacgt ggaaatgacc
      541 tagcagtcac cagctttttc ctttcggcag ctgcacagtg cctagctcga gagcaggaga
      601 ggctggcgtt tttatcaagc cgagggctca gaccaccaga ggctcttgtg aaggaaggaa
      661 attaccgggt gcaaacattc acaatacccc aactcaattc cccctccctc atctctctct
      721 tttccttctc tctctgactt ccactctcct gtccctatgt ttatcaggtt ttggggcact
      781 gtattttgat gtcaataagc aatttttctt gcctggtctc ctgcctgacc cctttaatcc
      841 atcaatatat tattagagat aaagttttgt ggacgcctgc tgcagtcaca agataaatga
      901 catctctgaa gtcacgcatt aaagactaga ttaaggcctt gtgactgtgt cttcagtgct
      961 catctgtgtg cctctgtcca acctatcaca ctaagtgaca agaaagaaaa ataatgaact
     1021 gaggcagaaa aagcaaagag atatttctta cacacacaac ccgcgtctgt cttcagccag
     1081 gattaggacg cattagaggc gtgctggaga agggtagaga ggcagcaatg gagcgtcact
     1141 gtttgtgtat gtctgactga aagaaaagga atttcaaccc tcaatccttt taaactaaag
     1201 ggaaacaggc gttttgttgt aaagatgttc cgatactatt ttttccttcc cgataccgat
     1261 tctgattcct gaactttatc ggcagattcc tggtaccaaa acgataccag tgcttaa
//
LOCUS       KCVB01000686            1498 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32553pri ultra-conserved element locus
            uce-1113, sequence.
ACCESSION   KCVB01000686
VERSION     KCVB01000686.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32553pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1498
                     /note="ultra-conserved element locus uce-1113"
BASE COUNT          365 a          353 c          379 g          401 t
ORIGIN      
        1 ctttgtggga tattgattcc cccatccaga gacggtttcc ataattggtt aaaatgaccg
       61 ggtgcagaga gggatggctc tctgctgaaa accatttccc agccctgcct ggagctgagc
      121 caagctcggg gttgttcgga tgctttccag ccccagcctg actggcagca attaggggtg
      181 tctgagccaa gctcagcttg cgctccacac aaacacccct aattactgcc aattaggcta
      241 ggcgggtagg cagatttgag aagccggggg gatgttcagg catgggtggg catggaaaca
      301 attttcaagg gcttacaacg tgactggatg aaaaggttaa tgctttggag tgcaggggga
      361 taataacaaa agaaagggga agcagagccc cggttctcac ctccagcgac gagcctggct
      421 tcttcttcag ctcctcgcat ttcctaaatt tgcagatctg gtggcccgtt ttgcggttcc
      481 gacaactgct gcagacacca cagttgatta gccgcctgca gggtgcacag accccacacc
      541 ttttcctttt cttcttcgct gggtttccac tggataccga ggaattattc tgtgggcagt
      601 ctgccagatt ggcaatttga aacgcactgt ctgtaacggc tgctgaggct gcgggggagt
      661 gaagggctgt catgacgatg accccgggag gtaatgagat gccccctaaa gccgggatag
      721 cggaaaaagt tccaacgcgt tctgggagat tcattatctc tgcttcagca gcgccgcatt
      781 tcagcttgtt catgcattct cctgctaaag gtctgcagtg ttcgggggat aaggtagaga
      841 ggaaattatt atttgccatt tgcagcgtct ctggcggcac gccaggcttc cccggcctct
      901 gggagtcatt tctatgcatg ctggtcctat tagggtttat tgccgctgat ttccttcccc
      961 agagcatggc gttatcgcag ttccaagggg acatgccaat tctagcactg ggaaaaatgg
     1021 gcgttgtgat gcgggcaatc tttgcagtct gtggaaatgc cccattggtt ttataaaagt
     1081 ttgcaaaaga cctgtacctt tccatttctg cgttataatc caaaagctgg cttaatccac
     1141 catcctgaag attatccttt tgtaagagag acacatctgc gttctgtccg ttctcaatgc
     1201 aaagcgcatt gtttatgtta gacatggctg aggaatggct gtcaatgttt cggagaagag
     1261 agtgaggtta cagtggcctt tgtcaagctg aagctggtta ccaacagtgt gttaccatcc
     1321 tggtttatct tctccagtgc taaaagcaaa acacaacaaa aacaaaagaa aaacaacagt
     1381 tacacataaa agacgcaaat gttttcattc taatcagatt tcattcctct gcttcaactt
     1441 ttctccgaag ataacagctg aacatttgaa gaatcctctg gcacattgtt tacgctct
//
LOCUS       KCVB01000687             888 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32588pri ultra-conserved element locus
            uce-649, sequence.
ACCESSION   KCVB01000687
VERSION     KCVB01000687.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32588pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>888
                     /note="ultra-conserved element locus uce-649"
BASE COUNT          274 a          171 c          192 g          251 t
ORIGIN      
        1 gtggtaccct cgctagtcct tcctttgtaa acccatgctt tacacctcca gtaaagtctt
       61 ggggcacagt caggcagtca ggactgtttt agacgcttcc tgggaagacc aaagggtggg
      121 cactgtgctg acgccagggc gcgaaaggcc tcggctacca agtgaggatg ggattggatc
      181 attgacttcc agcctgcagt ctccattata tccgtagcgt gactgtaagc acaggcaaaa
      241 tgaagttaac actacatgag attgtgcaca ttaaaaatca ataaacataa atgagattgt
      301 aatgacaaag caaataaggg gggcttgatt tgtgagttgc atggtaaact tgaaaaaggg
      361 ccattagtgc tttctgtgcg caggtccaaa ttaatgtaga gctagaaaat cttcatgtgt
      421 tgaaaacaaa agaagcagaa ataagttcaa catttcaagg tgccctttga caacagagta
      481 cgtcatctga aagaaaaact tctcataccg accacctccc ttctttttaa aattctgtaa
      541 atattccatt tcaagggttt atccaggtat gagtctatta ataacttgga tacaaagata
      601 tggagcttca aactgaaaag gcaaatagca ctctgtctgc ctgggctaaa acaatgaatt
      661 ctaatgcatc tggtagaggg aagagataag agtgtgttat taatcttgtg tcacatatat
      721 cagctaatta catacttgct attccagttc tttccatctt tacagcccag ctgtctcagg
      781 acactgcacc tatggcaagg agataaaaag gtaagcattc aaggctggta aacagaaatg
      841 gcagcaggaa tttatcactg gagttttttt tttttttttt tttttgca
//
LOCUS       KCVB01000688            1359 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32627pri ultra-conserved element locus
            uce-298, sequence.
ACCESSION   KCVB01000688
VERSION     KCVB01000688.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32627pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1359
                     /note="ultra-conserved element locus uce-298"
BASE COUNT          398 a          263 c          273 g          425 t
ORIGIN      
        1 agtactttac aggtcatctg aggccactca ttaatctgaa gcaggggcag ttaatacatc
       61 aatacacttt tggatttgtg atttttctcc ttaaatcata atctaaacac accaatttaa
      121 tatacactaa ctgttgaaga ttttaacttg tatcccgtgg tagatcatca tttaaatgtg
      181 tggacactcc aactcggatt ttgcgtgtca tagtgttgaa gccctgttaa tcagattata
      241 ctaacacgtt ttgtacagtg gatagattca taatgttaat gggatttgtc ccgcagattg
      301 cggcagagga cttgtcattc tgaatgcgtc gataatgatc atttaaccca aaacctgagg
      361 cagtaagcct gagattctct tgtggctgcg agggaatacc tggaactgaa cagacgcact
      421 aaacaatgtg ctgcacctgc aggatgatag attttaagta gattaatctg atctgcttct
      481 gtataaatac atacaaacac acttgtgctt tgttttatat ttggtcattt tttaacctat
      541 attgtaaaat cacacatatt ccttttagat taaaaataca atttgacagg ataaagactt
      601 ccacttcagc tcggtgctta gtctgtgtct gtccctttgg tggtgtctgt attaggacag
      661 tgggtgtcct ctggccatga aatggctgca taattgagtt gtacatggct gaacctgatc
      721 agagacggtt cactggttga gcgagactcc atgcactaac actccacgca acaacggtac
      781 cccagggcag cctaccatgc ctggttccta caatatttct ccctccatcc gctccgggac
      841 aagaaaggaa caggggggga aaaaactgct gattgcattc tgatagattt ttgcagcctt
      901 aggcaatgga attaaaaaga ttaacagttc attatgaaga agaccacatt gattaggcca
      961 ttgacagatg ggccaataac taaagtccaa atgtaacaat ttaaatgtca cgaaaattgg
     1021 cacttacctt ctcccaattg atttttaatt tcacctattc tctgctaaca tttgaaaacg
     1081 cgtttcaaaa gaggcataat gtggagtaga gtggccctat tgagttttaa ttggagggcg
     1141 attggaggat atcaaagctc tatcattaga gtagatggta tctttgcctt acagggtcca
     1201 atgtcaactc tgtcatccag gctctattaa gcatacaatg aaaggtcagt ttgcacatat
     1261 gaagtactct ttaggttttg cagtgttttt ttttatgatt tttgattaag taagaacaca
     1321 atcagcacca tcagtcgtta ccctagttta atgagttac
//
LOCUS       KCVB01000689            1993 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32670pri ultra-conserved element locus
            uce-38, sequence.
ACCESSION   KCVB01000689
VERSION     KCVB01000689.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32670pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1993
                     /note="ultra-conserved element locus uce-38"
BASE COUNT          591 a          354 c          375 g          673 t
ORIGIN      
        1 cttgaccagt tgacggacga gctccaggcc aatcagaatc tactaatgga gagtcagcaa
       61 aaggtggacc atcttaaagg cctgatggga tcactgcagg agcaggtgga cacacacaaa
      121 catcaagtta ggacaaacat gtacacactt acagcagcat atgtagacat gaaaacatga
      181 caggcatata tgcagacgtc gtcctttatc tctcagtgga gttcctgatt tatttgtatc
      241 atgaatgacc actgtcactc attattctgg aatattcctc tgactaaaaa aattaaaaca
      301 cctggaaatg gtgtgtttca ttgattatac ataaccttca ctcattttat gagagtcttc
      361 ccaaaatttg aaaaactgaa aagcttttta aatgaaatcc catctacttt ttcaaatgcc
      421 ttttcaaata aagcccagat ttttattcct tttcatagtg gtctggagta tcaaaaagat
      481 gccacatttt atcaccaata aagtggcctt ttatgaaacc tgtttgatca tattttgttt
      541 cataaccaat gattcttttt attctcaatg gaatgcattt agagaggata tgggtatcac
      601 agcagagcga cgtcagtggt ctccaatgtt taatatacat gggaactgta cgtgtaacag
      661 tctatgaggc tgctggggag atgatcagtg aaagcatatt ggtgacagtt tttcaaaata
      721 ataaacatat tgtttcaatc tttatagtgc ttgaatgtat tttttaagga tgcagttttc
      781 atgtgacctc gcttgagttc caaacacgaa gatttagatt tcatacttct ttattaaggg
      841 gttctcagtg ttcttgtgtg tgctaccagg tgcttgccgg ccccattaat tctgtatgag
      901 cgcatgcatg ggcatattct tttgtgtgta tgtgccattg tacacatgtg tatgtctgtg
      961 tacaagtgtg cttttgtgca tgtgtgcact ggcgtgtcac ttaatctttt ctcctttttc
     1021 tttctgtttt atatttacta cgtcaagcaa caaatgaatc atctttatta cggagaaatt
     1081 taatttggat gaacttaact gctgccgcca aggtcagacg ggcagaatgt cattttaatc
     1141 caatcaactt taatctgttg gttataaaag agttaggctg ttgaagaatg gctgtatctc
     1201 aggcctttta ttacactggc taatgacttt gattgtgagg cgcctatcaa gaggttagga
     1261 gttcttatgg agcttcacgt cctgctaatt ctctgcctca tgccactgtt gggctcagct
     1321 aacctgaaaa ttcactaatg tctgcattcc ctgtttacaa gacaatctta attgaaaaag
     1381 tgaaatactt ttcaattttt aaagaatgac tataaaatca ttgtacgaat tatagcataa
     1441 gtaaaataag ctgaaactta cacagtagct tgagtatagt atcttaacgc catctctatt
     1501 cgagtctatt tgtattctga ccagggaata attcactgta acggtaagat gagtacaaaa
     1561 agtaaaattt atcttcttac ccacaaagat gtctactttc aaattagtta catagtttgg
     1621 ctctacaaag gccagcagct aatagactta gactgagggt ggtgatacat ttaacaatac
     1681 attcagaaaa cacaatgagc tacatggtct tttaattaag agagtgaggc taagatcttt
     1741 aagtactatc atctatttta gtttctgact ctagattcac ttgttaatgt tattttagat
     1801 gtgttggagt ttgtcagctg tgcttggatc atcgaggcct gtgctctagg atgcaacaaa
     1861 tcaattcaca aatgagtgta atacataatg atgcgtttgc ttaatggcac atcagtgtca
     1921 ttgtcattta ttaactgcat ataatctgtt aaaatacaat tcattttaat gtgcttttca
     1981 tagatttgct cct
//
LOCUS       KCVB01000690            1775 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32715pri ultra-conserved element locus
            uce-754, sequence.
ACCESSION   KCVB01000690
VERSION     KCVB01000690.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32715pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1775
                     /note="ultra-conserved element locus uce-754"
BASE COUNT          516 a          391 c          362 g          506 t
ORIGIN      
        1 aacaagctcg tatccaccat agctcaacag acacagaact gcatcttcaa gagcagaaac
       61 tcagttgaaa aagtggtggg caaactgact gacaaatgat cactaggagg agggggcaga
      121 aaaactgtac aactcaggtt ttaaggcaag ctttgaagga agatacaggt ccctgtttaa
      181 attcaacaca aacctgaatg tttagaaatc aaacacaaat atctattttc agttttatgt
      241 tcttggtatt ttatcagata caagttttgg ctatttcgag ctactgtctc tgttgtcaat
      301 agaccataaa atggcaagaa tgattaacta gtgagttaat taataacaag ggagattacc
      361 ttgcaggcat tctaatgtgt gttatacctg tggttaacct atagccccaa cttcatttcc
      421 ctttcccaac ccaatctgtt cacaatcctc ttcatcacct taacccagta ttctcacctc
      481 atccttcctg catgtccctg ttatctagct ctgtactgag aaatgaagac agctgcagtc
      541 aacagttgtt actgtagatt gcctttaaca agagtaaaaa caacaacaaa aatctatgat
      601 ccttagtctt ttcttcctgt ttcaaccctt acaggcccac ctcgaggact cataaccttt
      661 aacctcaggc tcgacctgtt cctcttcctt tgcaccactc tcagccattc ttccctgtct
      721 tcattcccat tcgcttccca ttacccagcc acaatcattc tcctccagcc ttttcccatt
      781 ttaagtcctt tctctttccc tgctggggct ggagaggggc cgaagccaca gcatctcacc
      841 atccgttagc caggagccag agatcccagc cagagatgcc atctgataca agtctatcat
      901 aatgaaggtc cagcatgggg ctaaatgagt ttccacctca gccgcgcgag gggctaatga
      961 caaaacagcc tccctgtgct ccttggcctg ccgctagctt taatcagggc atatggcact
     1021 tgcctaaact gcttggcttt gaggaacaga gaggagagaa gaggattttg gagaaagtag
     1081 agaagagact gcagaggaag gtctgaggag atcaggaaat aaaaaaatgg gacggacaat
     1141 aggaggtgag tgcagagagt gcagataact gggtgagttt gaaaagtcgc agttgaaaca
     1201 acagtgtaaa atagtcctgc ttcatgcacg ttaatctgca gagttgatga caggcctgta
     1261 catctttgtg ataattgctt aggacctact caaggcctca taggcaggtc tttgtgggtg
     1321 cctgatacgg ggcgtaaatc actaatagct ttcatagatt tatattaatt tgcaggattt
     1381 aatgtgcctt aaatggcaga tgatcaacaa ggggaaacaa aagtgcatta gtgccccggt
     1441 ggcataaaaa gagtgtggaa gggggtggga gcttaacaaa tagactactc cattttcata
     1501 gcccacttta tggctcccca acttatccaa attagcttaa aggattgata attcttcact
     1561 aatataaaac tgttactgag cccctaaact gtgacttgcc ctttttttag gttttgtcaa
     1621 acactaaata aagggcttgg ttgtggaaat taaattgaaa tcgcataaca aggcttcctt
     1681 ttaatttgat taattctttg ttgttgtttt agatccttag tgaagacacc catgacgttt
     1741 cgaggttatt tgttccactg tctaatgaca atttt
//
LOCUS       KCVB01000691            1591 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32757pri ultra-conserved element locus
            uce-378, sequence.
ACCESSION   KCVB01000691
VERSION     KCVB01000691.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32757pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1591
                     /note="ultra-conserved element locus uce-378"
BASE COUNT          491 a          329 c          330 g          441 t
ORIGIN      
        1 tttaaaaaaa aaaagaaaaa agaaatacag taatagatca aagatgaagg gtgaaaaaat
       61 atctctgatc tttgtaccag aacagagaga aatagaaaat tctttaacat tttgaccaaa
      121 ggctgttttc tttttgctgt ttttgacaaa aaaaaggcat aactgtggta tcagaatcag
      181 attcagaatc agtattattg gccaagtatg tatacacata cgagaacttt gactctggtt
      241 tttacattgc tctcaaggca cttatactgg aacagacata acagctaaga acaaggacaa
      301 cacagccaca tttcctattc ttattcatta gagagaatca tataaataaa taaaatatgt
      361 tgtctcgtag tcactcatcc cccccaaggg aacatggaag ccccagtaat cacatatggc
      421 acaatagctc gcccataatc ccctttattt ttattttcat gatgtcactc tctttaagcc
      481 ccttgcttta tgagtcgcaa acggagtgag gagtggagga gaggaggagc ggagaggaga
      541 ggaaaaagca agggaggggt gaaaacaaat gatgtggatc cctcattgtt ctttctctat
      601 gccctcattc cagtcagggt aataagagaa cattgtgatt tactgtcagt gaagcgcaag
      661 agtgcatcag agagaatcca ctgcggcagg ggccattaaa atagatttaa cttcccacag
      721 tattaggcca gatgtctaca cttctattat atttcaatca gccatgacct tggtctccat
      781 atgatgagag agatcactga gagagaggga aagagagagt gaaaatacac gcagaaaacc
      841 aatagaaata tcaaactact tagtcccact aaatccctct gctccgtcgt gcagcatata
      901 cagagagagg ccgggaccga aatagagcca aggtttcaag ttctcttaaa agagtgtatg
      961 aatcattgag tgcactctca ctgctgataa gttgctgttt tttgggggga gaagtgctgc
     1021 ttttctgctg gttatgtatt tgaggaggga cagagggtgc acgccaaaaa gagcgggaca
     1081 gagggtccgt ttagatgttc tttaaagcct ccgatatcga caggcgtcat gaaagacgga
     1141 tcttttcata tttgaacaag ggaaacattg acttctcaca tcaccatgga tcccctgtct
     1201 ttgacatttg catttaatat ttttttttaa tcctgctgtt tactctcgac tcgctaatat
     1261 atctctactc tcaataaata aaatccttac cctgccacag ggtaagaatc tccattcatt
     1321 ccatccattt tagccccatg aaagcagagc agaggtcaca gcttgttctc tttcagaggc
     1381 tgatggtaat tggacttatt ggctgacatg aactgaccta cataaagaac taccgatccc
     1441 cggttacaac tgtaacttac aatccccccc tagcatgggc gtggatgttg tgctgatgct
     1501 cctctgatgt tcctccagca ttgttttcag atattgacgc cccagcgctg gtttcaaacc
     1561 gaacactgtt ttcagcctga attcatgctg c
//
LOCUS       KCVB01000692            1479 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32798pri ultra-conserved element locus
            uce-1154, sequence.
ACCESSION   KCVB01000692
VERSION     KCVB01000692.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32798pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1479
                     /note="ultra-conserved element locus uce-1154"
BASE COUNT          402 a          288 c          367 g          422 t
ORIGIN      
        1 atctattaaa tattgactag tggagcacca taacaccgta atgtagacag ttcaaaactc
       61 tgtttcttcc tgtcccttac acaccacttt gatatggcag acaaagaaaa caggtagctt
      121 ttgccatctg ttttttattt tgtgtaacac tcgccaggat atttgggagg attttttact
      181 tgaaagagtc acctctgctg ttttttgtag cccaaaagca tctttctgtg tgctggaaaa
      241 caatctagca gattaactaa atcagtctgt tgctaaaaaa gttagaggca gccagaaggg
      301 tacaattgtc ttagtggctg gttgactcat gacatatcta ctgtataggc ctatttaaag
      361 tggagataat ctattaatgg ctgttttatc gctatagtaa taaaacaacc ttaaacagct
      421 tttctttttg gttttatgtt gatcaacctg agcttcccca tgtccttgtg aacacagcca
      481 accccggtct actccacact gttgggcttg gcctcaggca gcacacacac aggcagacga
      541 agctgtggga ggtggggttt ggagggggaa cagaggaggg cctgaggggg catgatgggg
      601 tgggaggcag ggggaacgtt tgaagccggg gtgaggtctc aggagtaggg ggcggtgggg
      661 ttgggtgggt gtgttaaggt gataaaaacc tttcatgaca ttagggtctg tgaggttccg
      721 cgagcagatc attgagatca tagcatggag cctatcttcc tcttcctcgt catcgtccag
      781 cgacggagcg cggctgcccg tggtgtggtg gtagtttata gtgactgctt gcaccatcgg
      841 gacaacagag aacacagtta gaacagtgcc accatacaga cctcgggaag agcatcttgg
      901 gaaaaacacc acactacact tgtactttgt aatcacatca tgggaagaca aagttcaagc
      961 agatataaag tacttaattt tcaaaaggca aaagcaatca cagagtgtta cccgtagagg
     1021 gaagattaca tgtttggtgt gtgaagctgc cactgccttg gggatgatct gtaccactgt
     1081 gtatgacgat gtaaaagttt tacttactgg cgtcgtagcg gtatccaggg tagacgatgc
     1141 gaaacacgta gtcagcagct gtctgctcct caccgttcct gtcctggtca atgagtctgg
     1201 cagcactgct cctcttcctc agtttaggaa acactttccc ctgcagagca aacaaaaaat
     1261 gtgttgaagt gtacaaaatg ctttttaggt tcttccttgt aagctattga caagcagcac
     1321 tgcttcagtt aaatgttatt gttacgctct gtttggttcg agttgcagtt gtgggaaaat
     1381 ggaaacaatg gaaatgtggt gttcatattg attttctttc aaaagtacat aatatgagat
     1441 ataccaaatg atatctaatc taatagaatt ttaagttca
//
LOCUS       KCVB01000693            1241 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32842pri ultra-conserved element locus
            uce-1111, sequence.
ACCESSION   KCVB01000693
VERSION     KCVB01000693.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32842pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1241
                     /note="ultra-conserved element locus uce-1111"
BASE COUNT          274 a          310 c          327 g          330 t
ORIGIN      
        1 aatttgaaga cagctgattt ttttttggcc agtggttaaa gtttggtaag tagtggttag
       61 ggttagggta agtctccagg aaatgaatgt aagtctatgt aatttcccaa aaagtgacca
      121 aagtaagtaa gtgtgtatgt atgtgtgttg tgtgtgtgtg ggtgcagacc cccctctggt
      181 ataaattacc tgctgactga caggccaacg gctgtcagcg aggatgtgct gatgggcaaa
      241 cagtgacaca ctcctcccct ccgttgccac caatcccgtc ccatcagcca ttcctcactc
      301 cacttccttc tcccttcctc ccctctcgct tcagcctccc tctgtgcccc gtgatgcgct
      361 gcggtaggtc aggcaggcag agacggtgag caaaaacaga gaaaggaaga gaagcagaaa
      421 ggcacacggg gtgaaatggg agacttttat tatcaccatt gtttcaagct cactgattct
      481 cccccccatc actgactgct catatgtcct gtcttttcaa cacacctgag ctctcccttc
      541 actctcttta ctcctctgtc cccaccgcta ctctcctctt tctctcttga atctcagtgc
      601 ggcaaatccc tccttacccc cacacaactc ctccactccc tatttgctcc tctcctgttt
      661 ctaccttccc tctcaatggg ctcccactga gtttggtgga ttggggaaag ggggaggaaa
      721 aagaggggga gagaggtaga aagagagaca gagagagaag gagggagagc agcaggcctc
      781 tggctgtaag ggagagtgca cctggatggg caggcttcct ggacatgctg tgaggggcta
      841 ttgtcctgca tgtcagcagc ccctctcttt taatggggcc cgtatatcat cgttctgatt
      901 atggcagatg actgggactt gggggaggag gggtggagat ggtagatctt ggagagacgt
      961 atatgtgtgt gtatgtgagt gagagagtga aagaaagaga gagtgtcttg gggagggagg
     1021 tgggatgaag gcgaggtttg gggacagagg cttaaaaacg cagggacccc caaatccccc
     1081 atcgactgaa atcctagcac ttaaccccag ctatggcatt gtgtgctgcc gtggggattt
     1141 tttcctctct ttttttgtgg ccgtcattct ttttaatgga ctctctctcg ctccgtctac
     1201 ccctccctct ctccattcac ccggtggatt ctttagctga g
//
LOCUS       KCVB01000694            1774 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32917pri ultra-conserved element locus
            uce-960, sequence.
ACCESSION   KCVB01000694
VERSION     KCVB01000694.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32917pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1774
                     /note="ultra-conserved element locus uce-960"
BASE COUNT          472 a          403 c          376 g          523 t
ORIGIN      
        1 aaaatggctg cctgaaagtt tacattgaaa gacagtcagc gagttttggc cggggtgata
       61 ttgcactgta ggtgccaaca tgatggcaat tttgaatcaa aataatccca tttgttctag
      121 tggtgtcaga tgttctggtc cgcagactgt ctacctaatg aagtaagcat gcaagactta
      181 tgctgtggtt ccattgcact aatgctgccg tttctgctgg ccaaagactc tgcatggttt
      241 tgattatcac gtatcgttaa aactcagtca ctctccctcg caacatttgc caacatgctt
      301 tcaattcagt ctgtctgcct gtccacagaa gaatatcttc tgccacagcc gttttacgat
      361 cagtgataat tctgaaaaat gatgtctcta tgccgtcttg tcgtatcaca aaggcccgaa
      421 agcgaaatct tactgctgat gttgctgatt taacatcatt ataatgtaat tcaggataaa
      481 gccctgaatc atcgcatggt gatagtgtaa cttgagcatt aagcacagaa tctgcttctc
      541 ctctcagccc tcttgaactg tttacagtca tactggaaaa cacaccagca caaacacacg
      601 catcaacaga gcagggctga gcagtgatcc actgtttgct gaccaaatga ctgtcaccct
      661 ttgcatggga aggggcagag gcaggaagga gggtggtggc agcacgttct gcgctgctat
      721 ttgttaccat acagttgtca ctaaagtcat aagttagtct ttgaggctaa tcacagctaa
      781 ctcctcagaa cctcaacagc cctcaccgca agccttatac cacctcgtct cattcccaca
      841 ttcctacaac cctctttcct caccactccc tcatccaccc tccgcagccc catccgagcc
      901 caccctcacc ttctcaatcc actcttggat catttcaatt tgcatcccaa ctggccccgg
      961 gattccctag ccctgttaaa aggcaggcta tttgttgtgc tccgcggtta atgccgtgca
     1021 gagctttaaa tacccatatc aaatcttgga ggctcaacag attcagtttt gcaaacctgt
     1081 atcgaacaga ggttcaatcc gtcagcttag aagtgtacac tccttaagtt gtgtgataag
     1141 gtaaaaccaa tcactggatt aggctctctc tgtcagtgat ggtgtatttt tagtgcatag
     1201 cgttgctttg ctgaaacagg ataagctgat ggaggaacat tatggtgctt gagatttctt
     1261 tttcaatagg aatatactat taatggattc tggagtcagt gtatgatgcc gctcttgcag
     1321 ttgtggataa tgcattagca ttagctgtga aaagcagttg gggccggata ccatgagcta
     1381 tcatctgtca aaaccacaag tttgattgtt tttatcatgc aaatccagcc tactaaccga
     1441 taagattgat tgaggactta aagtaggatt gcagtgttta ttctcataga gcttctcaca
     1501 aacaaacctc agcctttaga ttcacaagta taattttgca aagcatatgg tgaattcata
     1561 actcaagaaa gagagattct caggagtctg ctgctttttg tggtttttct gccttgttcc
     1621 atctggtttt gggggagatg tgggaggttt tggtacatgg cgagcttgag tgaaaattca
     1681 tggcaggtac ataaataatc acccagctaa aatataggct acaacttctg tttctggtcg
     1741 gagtgttgca agactgtctt ggatgacatt tgag
//
LOCUS       KCVB01000695            1994 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32951pri ultra-conserved element locus
            uce-925, sequence.
ACCESSION   KCVB01000695
VERSION     KCVB01000695.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32951pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1994
                     /note="ultra-conserved element locus uce-925"
BASE COUNT          582 a          435 c          364 g          613 t
ORIGIN      
        1 cacacacaca cacacacaca cacgtacatg taaatatata tatatatagt acagtcacac
       61 gtcacaaaca tgcacacgcg cacatgggta tatatacagt cacatgcaca tataaacaca
      121 aatatgcaca gtttgtgtaa acacagataa ggtgcctggg tacaaacatc aataaaactc
      181 tgacagatgc tgtcatctgt gtaccgtatc catccaagca tcaataaagc aacatcataa
      241 acagtcctat tgctcaatga atgctctcca aaatttgtaa aagctatgca atggatcata
      301 tctaagggcc atgagggcct gcactgtacg gaatacatct tcagaggcat ttctgcacaa
      361 accctcggcc agtctcacag ttatacagtt acatgtcatg actcacagta aattgttcac
      421 actagtaata agaccagctc tacatgagag cagcactgat gcctgtaata ttcaagacct
      481 aatgtaaagt acctgtgtaa agtaacaaca aaaaacctgc atgccatgca tggtctgtgt
      541 ctctgaatgc gtgtgattta cacctgatgc taccaagaat gaatgctatg tgtgaggata
      601 tggatgtgtg tttgtgtgca tgagtgagtg ggtgtgtgtg tgtgttagtt tagataaaac
      661 agttaatccc caggccacca gaacgcaata tcctgtctgc ccgtttctca tttcctgtct
      721 gtctctggcg gagtgggaag cagcggggtg ggggtggcgg gggacagcgg gacccgttgc
      781 cccagaaaca ggagatacag ccggtcgatt tgagaaaagt ctatgccgat ctccctatct
      841 ctccctctct ggtgcgaccc cctattctct ctctctttct tcttctccct ctctgctttt
      901 attatctcag atttaataaa gccaaggcga gcagcttgtt ctgccatgaa acatttatta
      961 gctgtctgtt ataaaattgt tgtgggggag gaaagatgat ggggtctcat ctcacattat
     1021 gcaaaaacag tggcaatatc accccctcct ctcacatata cacatatgcc ccttcctgtg
     1081 gctgtatctc tacctaaaac tctcagacac cttgtcttta ccttctttat cccattccga
     1141 aagctttctc aattagagac atttctccct ggtgtttttt tactgatacc ataaaatctt
     1201 actgttctcc aatagtgcac catcaaaata caaaaactcc cccatcccta ctgtcctttc
     1261 ctcttgtaca cctctaacca tcatctgttg agttgaaggt atagctaatg taagtctgct
     1321 gggtaggaga ggagtagttg aatgtttcag aaccacatac aggtctgatg tgtagccttt
     1381 cactgggatt catatatgca gtacagtcct gagctgtcag tgcctcaacg attcacctaa
     1441 ttcgtatctg aaccacaaac tttccttcca tttgcaaaat tttcaattgt ttaaatatat
     1501 attttcttat acatataatt tgcagctaag gatttctagc aatacagata gttttatttc
     1561 attagccaag gttctgagaa tttctgagag accctaatac aaccattaaa atttttctga
     1621 aaggacacat aactgttgat tttttaaaaa tgtttttttg tttttaatat gcaacttttc
     1681 attgctgtga gaaccacaaa taaaattcta ttgacctcct ctctaatttt aggggtgaat
     1741 atctcataac ctcagcagct aaaaccgaaa ctatctgcat ggctagatat cattgagcaa
     1801 gaaatgcatt gtcttgtaaa tttgttaaac agacatttta aactgtgaca gtttcagtat
     1861 tcacacttga tatattaaag gtaattaatt aatatggcac tgagtcatta actggagttg
     1921 agatgctagc tggtggggaa aagagcttgt tctccttttg acgtgtcatt gcataattct
     1981 ctctaatagt attt
//
LOCUS       KCVB01000696            1710 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 32990pri ultra-conserved element locus
            uce-222, sequence.
ACCESSION   KCVB01000696
VERSION     KCVB01000696.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="32990pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1710
                     /note="ultra-conserved element locus uce-222"
BASE COUNT          488 a          354 c          361 g          507 t
ORIGIN      
        1 tttaggatgg aacaagaaga gaagccaaca ccttcgtcta gcagggtacc tgagctgtta
       61 ctcactctca aaacaacagt tttctatatc aaggtgtgtt ttatcatttt atctaatgat
      121 gcgagtgatg gattcacttc catgtgtcta tcaatgtcag atggctctct tgaaggcaca
      181 gaagcaagta aattcaaaca aataaaagga gatacttaat cttgacctgc actacaacac
      241 attacctgta ttttgaaact accagaagca taaataattc cgtctacagt ctacaaaaag
      301 tcagcaggca aagattaaaa catgctattt accgtgcaat tacacaaggt atgtcggaat
      361 tttttgttag taaagaatgt acaattcaca ctgagcttaa aatgcatttt cacagccata
      421 gaatgactct tgtgcaccat ttaattatga tttttccaaa taatgaaggt tttccacagt
      481 gtatttttta tgacagctga caacaattaa ggatgtgagt gattgttttt agagcgggtg
      541 tcgctcagct accccccatg gcagaggcca ggatgtatgg gagtgatgtg tgcgggcgtg
      601 gggtacatga cggggcgggt aaaaggttaa cgtgaatatt gatattaaag cgccgctgct
      661 tcatcatgtc tgtgtcagcg agtgaaacgc tccttaaagg tcatccctgt tctcctctct
      721 ctccctacag agttccactc actcgctgtc aggccgtggc tactcggtaa gtggcatttt
      781 catgcttcat agctttacgg cagaattaca atctccaacg acccgcttta tggccccggc
      841 gctcgacatt ttaattatgt taaatagagt aatggagaaa tcatgatatt aggaacatta
      901 attctggctg actgactgat taaattgcgg gaaggtgtcc tgcgctacct tggcgaaaag
      961 agcctcgttt tattggcatc gatcagcgtt ggtcgccggt gctgcaattt ggctgtcaga
     1021 gttttatgtc ttcctccagt gactgtcaat ggaggatcct tttgctcata tgacaatatg
     1081 cctaaaagcc ttttaaaggc ccccatatca gtgcaataaa tacaatttct cagcagcaca
     1141 gaaaggcatg tacattaaca ccttcaggaa gatccatggc agttaaacat gtcttatgat
     1201 ttgttggaaa caactgcctc tacattaaga ggagtcagtg cgatggtttt tgtcatggaa
     1261 catgtagagt gtaaatatgt cagagaggga tatttagtga tgccttggaa aacgtcaacc
     1321 cactcctttc aaacatacac tagctctcaa attgtcagag ctggactatt tttatccctt
     1381 gaaagcatct gttgccttgc cccagtgcat ttgcgagccg taaatttgtg ctattcccct
     1441 cccattgttt caatgatgtg tctagtctct ggataataga gacagcggtc gtcctttatg
     1501 tcctttgctt aaaggggaac tcataagagg aacattcttt aaagtgtgct acttttagct
     1561 gatgagtggc atatggcagt tcttattgca gcttggaagc aatcatcccg acaaacaaaa
     1621 caactataaa tggtttgtca tcgttaatct gcctcaaagg accgcagtta aagcgctgaa
     1681 caaagcctaa cttcaaaaga ccttgatttc
//
LOCUS       KCVB01000697            1247 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33031pri ultra-conserved element locus
            uce-267, sequence.
ACCESSION   KCVB01000697
VERSION     KCVB01000697.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33031pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1247
                     /note="ultra-conserved element locus uce-267"
BASE COUNT          379 a          261 c          238 g          369 t
ORIGIN      
        1 ttcactacaa gttaatgttt agcctctaca ttatttattg ttgtttaatg aaaatgttga
       61 aaaagaagca taaggacaat caaatttatt gccatatttt catcatcatc ttactatgta
      121 acgataacca aaaaggaaac tgcatcatat caagagtaaa ccacactgac tgtctgttat
      181 attcaataaa aagcccaaag tcctgctcaa atatttgcct aactcaactg ttcagcacaa
      241 gaagaacaat gatttgtcta atttcgcatg gctatagaac aatgaattgg aatcagattt
      301 gaacaccccc ttaacatcca tctctccagc gatctatcca ttcagttgcc tatccttgtc
      361 catctagctc ggggctatgg aagcctaatt gtttaccaaa gggttgcggc tcatttgctc
      421 ctggagattg ggcctaatta acactaactt gtctgaacac cgaggagcgc tgtccactcc
      481 actttaatct aatctgtact ctttcaaaaa ataatgagat catttacacc tcattaacga
      541 gcaatgtaat gtctgggaat taatcataat gattgcttat catttcactt tgcaatatta
      601 tctcctctaa tgaaactgtt cacacaactg ccaagagggc cttaattggt cgtgtgaaat
      661 cacagagcac ccggcagctt gccgcgacgt gtgtgaagtt ctaatttgca tggacggggg
      721 tggaggggaa tttggggggt gggcgcgagg ggatggcatc acttaggaac acacgcaaat
      781 acacacccga taaggtgtac agaaatgttg cggtatggat attactgtac aatatataga
      841 ttaaaatata gaaattccca ggcagccctt taggatcaag tgaatatttt ggtgaatttt
      901 tactttttgg attaaagaag agacttcaga ttataagaca ctttcaagtt atttttaaat
      961 attaagtttg aaaccaaatg tttgctcctg gttcctgttg tccgtcttta tggcataaga
     1021 tccatggcag tggtggatgt gattattcaa gtgacatcca gtggcatcca gactgatccc
     1081 agctttgtgc caccaccgtg aagccaagac atgaagaaca cccatccaat attccagttt
     1141 ttgcattgtt acctcttata acctacagta tatccaccca gctggtcaac cccagcactc
     1201 tcgcctcctg gagggaggct aaacagtgct aatagcagtt aaaatat
//
LOCUS       KCVB01000698            1718 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33074pri ultra-conserved element locus
            uce-457, sequence.
ACCESSION   KCVB01000698
VERSION     KCVB01000698.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33074pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1718
                     /note="ultra-conserved element locus uce-457"
BASE COUNT          499 a          359 c          347 g          513 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
       61 tgtgtgtgtg tcgcagtgaa cacattcctg gaaaaacagt gaaccttcag ctttgacaaa
      121 gtttgcaaca ggaacacttc caaagctgac aactttaaag gaaaaaaacc caacaaaaaa
      181 cgaaggggag atttgaataa tacataaatt gaatgtgaca catttatttt ctttacattt
      241 cctgggaatt cattttattt ggcttttaga cgtgactaaa aatacatatc taaaaaattt
      301 cagattgtga aaacattaat aattagatgc cttggagatc cagaatgatt tcacaaactg
      361 acgtggcatc caaaaacaac accagcgcgc agttggcttc ctgcaggaac ttcatatttt
      421 gtaatctggt cttttgcaat tcacagctga cattgttccc ccacaagaaa ggaaaggtta
      481 cactaaaatg tcaaaacatc actggacaca aaggagaagt caaatctcac tgtcttcttt
      541 ttaaaggttt ttccaggact ctataattgt acttttcgtg tcacaacatg aggttaaaat
      601 tgattaaatc tccaaattca cgacccatat tatcttcatt gttgttgcat tatattctca
      661 tgtgtaaatc cttaacacag gctctgttca gcagctccca aaatgtgctc tccatcctgc
      721 cggtattacc ctcagatctg gttgttgctg agctcaaaaa ccacagaaac ggcgtcagga
      781 gtctctcctg ctcaccatat tgaaagtgtg ttactgtgtg ttcgggagag tgtcggtgcg
      841 tctgtgagtg tgtgcgaggt aaaaagtgtc aaggaattca ttgtctgttc tccacctgtt
      901 attattctgg tcccaatcaa cctgctagcc agtctggagc cagtggccat ctggaaacac
      961 tgctctgttt ataaacttgg caaggatcag cactccgggg ctctgacctc ccacacaaat
     1021 ctggaaacaa ttagagcaag acagtctggc tgattactgc gaaagggccc agcgtggatc
     1081 ccactgacgg ctgcagggac gccagaccct tgttagagaa aacgcgaact cagtgctaca
     1141 caaaaccccc acatgattat cacacagaca cttacacttt aaggattatg tcgccatttt
     1201 catctagagt ctctatttct gtattttatg acctcttttt ttttgttctc acagacggat
     1261 ggacacacac agccacccac acgcacaaac aaacaggatt tattgatctt ttttgcattg
     1321 ttcatggaat gacttgcacc tcgcttttat ttaaggaaac tggctcccat aacttctgtc
     1381 cacatgttga ctcacgctcg tgaagactgc caacataaaa tctaaattta gaaaaatgcc
     1441 tttgaaaaag gaaaacaatg aaaggaaaag agaaaatctt gagtaggata attttttttt
     1501 tggtcatgtt taccgtctat tttttaattt gttgccataa ataaccttta tgaatctcaa
     1561 agtcattatg cacataccct aaccgtgcct gtgtgatacc ataatgctcc agagcgtggg
     1621 atccaattca atttggtgac agctcagaaa aaggggtttc ccttgcaaaa gagcagggag
     1681 tgaccttggg tcacttcaga tcatgacgtt attgatcg
//
LOCUS       KCVB01000699            1639 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33104pri ultra-conserved element locus
            uce-82, sequence.
ACCESSION   KCVB01000699
VERSION     KCVB01000699.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33104pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1639
                     /note="ultra-conserved element locus uce-82"
BASE COUNT          552 a          266 c          364 g          457 t
ORIGIN      
        1 accgactcag attggatata aggcctttta tgttttgtat aatttatctc ctttttttga
       61 ttgaagagag cagataaaat gttacactcc cttgttgaaa acaggccacc acagttcaaa
      121 aagaaagcca gagcaagact gcaaaataaa actgtcactg ttttacgttg tataagatag
      181 tgcaatgtct caagcttcac cagcaaacac tggctatgaa aaagggcgag agaaaatcaa
      241 acactacatg aatatcagaa ttttccttca gaaaaataga caataaaaac agaaaaacag
      301 aataaaggaa atttggagta gtagttccag aacacttctt ggtgagttag tgcagaaagc
      361 agcactggtg cattagtttg ttaggagtct ggttagtact gcacagcatc acagcaggtg
      421 gcaggttaat ttagctgagc gttgtcttgg tccaaagggg ccacagtggt tggcctcagt
      481 gtctgtgtgt ctttacatgt gtgtgtctgt gtgtgtgttg tgcgtgcgag caggtaatat
      541 ccttatgtgt aggaattgag gcattccttt gagcgtgagg tgatgtcctg cagctcccgc
      601 tcttccttca ttttgatgtc ttggtaggca tgcatttgac tggcatcctt aaggtaggcc
      661 cagtttgctg agagaatcat gaggaagtgg atctggaaga gaagggtgag gtggatggcc
      721 ggtgagcatg tcagagatgc aaagcaagct acaggagtca gagctacatc acagacatac
      781 gaaaacataa agttaagctt tttttaaaag acttaacatg agagtgacaa aagattacta
      841 aaaaaaaaga gacttaactg tgcaattatt gtaacattac tgttacaatc attactaata
      901 ctggagttat gttgctattt ttgctgtgct aaatgctaca gaaatgacac ttcaaaaggc
      961 gtgagaatga gaaaggaaag agacagaggt ttatgcatgc aagtttcatg cagctttaaa
     1021 agctcggggt tagtgcaaag catgagattt agaattttgt gaatctgacc ctgtcccaaa
     1081 tgcagttctg tattatactg ccgtaggatt tacaagaaat agaccaagta gggatacttt
     1141 tgtaaacaaa gattgtttat tttatataga aagtaaaagc tacagtcttg aaaaacttag
     1201 gagaaaatta aatatatgaa atatggagta taagtaacta taaacagacc aaggaatcag
     1261 agatgtaagc atgcaggctc agtgttaagg taaaaacaaa tggctcttta atggcacttt
     1321 gagagagaat aaaggaaaat gtatgaaaca aaacatcaac ttcatagctg aggctatacc
     1381 agcgtcagcc ctcatagaaa aaaaatacat ttactctact ctaaaatagg agaatttcat
     1441 ttttttatat agtacatgaa attaaagtga ggcttagggc ctcatgcaga agtatacccc
     1501 tggtactggc ctaacccatc agagcgtgca gtgctaagta tgtgcagtta ctctgaaatc
     1561 tacaagaaac accacttgtc accataatac tcatcagcgt ttaacagttg atgaaaggtt
     1621 tttagttcag tttagaatc
//
LOCUS       KCVB01000700            1755 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33181pri ultra-conserved element locus
            uce-387, sequence.
ACCESSION   KCVB01000700
VERSION     KCVB01000700.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33181pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1755
                     /note="ultra-conserved element locus uce-387"
BASE COUNT          553 a          389 c          356 g          457 t
ORIGIN      
        1 atccagagca ttgtagcttt tgagtgacac aaaacgtcac tgatgcacaa ggtacaacac
       61 gtccctgaga taagacagca cccagaggac acctgtgact cctcaagaga tggctggtta
      121 ccaattttaa tgagcatatt cataagtcct atcgaatgtt tgagccgtga atgtttaccc
      181 agaccatgag atatgaatga tgaacaattt gacctttgga aacagattag aatttcaatc
      241 aagtgaccac aaaccaatcc ataaaactat aaaatggaga gccgactcat gtaattacct
      301 ctagcttaaa acatcttaat atgtcctctc tcggttttta caagtccttt tggcacatag
      361 taagcataca agtagtaaga tataaattgt gtataaatca gaattctttc tactgtggtc
      421 atactcgact ttatccctaa gctatatatg ctccgtacat agcagcctgt cagcttttgt
      481 tcttggggcc gacctttgcc tgcacaggcc tcataactga cattttcaat tacactgcaa
      541 acaacttatc aatatgcatg gatttcctag ccaatatcag agcaaacaga gtcgtttgaa
      601 gagccgagtc tgtcagggga gagaaatagg gcagacatgt caaggtcagg attttgttaa
      661 tgagggacgg gccgctgaat aattgaatgg gctgagcagt atcgactgtt gaagcagaag
      721 cgtttaaagc gttggcgatc gttaaggaaa atcctcccag cactttatgt tccatggcag
      781 taatttatgc acgggcatcc aagcgggtgc cctatggttc acagcactga atgcacaaag
      841 caatgagtgg attagccaag cacactctag aaacagcagc caagcgtgca agtcattcct
      901 gtggatgaag taatgactct ggcagtgcaa ttattcagct gtgcatttta aatacacaga
      961 caaatggcaa cgagagaagc gtggaatatt tgctctgtgt accttgcaga aacatggaaa
     1021 aattgctaat tgtgtacaga aaaaaaggct acaagtgatc aatctgcaaa ttatgtgtta
     1081 cgcaaagcac actcaaatac agaaaagctg cagcattgac agagggcgat ccagagcaaa
     1141 ggaaacgtaa catgtgattt ttatgctgtg tactctgtaa acaagttcct aggaaaaaaa
     1201 aacatgatga atgactaaaa tgataacatc agctggtact gccggattcc ctggcagaca
     1261 caactgtgat atttggccgc agaaaacaaa ttttgataat tgcgggatca aagcaacaga
     1321 tgtaaatgca tgtaaacaag ctaagtaata cttctttagc caaatgcccc ctttgttttg
     1381 aacgcaatct cagccatttt taatctgtac cgagaactgc tcaagctgtt gttactgcag
     1441 taacatactt gagcagttct caggccttaa agcctccacc cctctgtagg ttaggagctt
     1501 tggggggctt ctaatccctc tatgtaatta ctgctccctg caatggccag accatctctg
     1561 tgtaaatcag attagctgac ctaaatcgct ctcgtttctc tctctacccc atcaaacaat
     1621 tccttatcta cacccagaga gctaaacaac acacagacac cactcctcca acctcccgcc
     1681 cccaaagaca tggtctgttt atttgatcag cgcaggaaag caaacagttt ctccatgata
     1741 cacaaacagg ctggg
//
LOCUS       KCVB01000701            1460 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33262pri ultra-conserved element locus
            uce-713, sequence.
ACCESSION   KCVB01000701
VERSION     KCVB01000701.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33262pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1460
                     /note="ultra-conserved element locus uce-713"
BASE COUNT          389 a          322 c          336 g          413 t
ORIGIN      
        1 atgactacag cagagtcatt gacccgggaa taaatgccga tcgtacccat tcccactccg
       61 gaaaaagcca gatgatggtg gtgttagtgt tgttgttttt ttgtctggtg tgaaaggggt
      121 attagttcaa tgggtcactg ggatcaagta gaggcagaac atggtaaatg atcagctaat
      181 tcactttaag tctgattcca ctcacaccta cagcactttg atatctctaa tatcccagca
      241 gagccttgca gagagctgtg aagtctcctt ttccactaac atcatcagca gcccctgtcg
      301 atgtgttttt ctatatataa aatcatcatt tacatcttct gcactgtctc ctgttggcat
      361 gctggcccac tggcctggtc ctcaaaccac taatcaggta atgttgggga ggttgaatgg
      421 catgcctccc cctggtcaac cttcactgct cacacagtgg tgttgtttta aagagcattc
      481 tttagcagct gaacttggaa aggtattaaa gtgggattag gctcctccac aatccactgc
      541 ttgtgtcatt cagggagttt gtgttctcct ctccccactg ccacctcccc actcctcctc
      601 tgtttgattg tggctagccg caggagtggg actgactgtg cctttcaaag gagaggggta
      661 gggttaaatc cctagtccac tgctccctgc taattctcct tgttccttag agaaggattt
      721 acttctgctg ccagcttctc aaaggccttc tctgcccatc cgaaaagtcg ccgttgcctt
      781 cctgtggagg tgtggttgcc atagaagggg cgtcatgcat ggctattcca tctgaacccc
      841 gctgagaatg gggaccggcg actgaaaaca ggcagaaagt ccggattaga cccaaggaca
      901 aagcaggaca ggaagattcc agaagggacg aataggaatt acagcagcaa aaataatgca
      961 aagtgacaga gaggggaaat agaaaaataa tggcttacaa gattagactc ggcgaggaga
     1021 aagaagaaag gagagagggt tgaatgagaa tgttttcatc tagatgagaa gatttgaaag
     1081 agtggtcgaa ggtgaatgcg gagtcctgga cgtatcaaaa ccgcgctaac aagtcatttc
     1141 ctcaactttt cgctctcttt ctcagcttaa gccggcaatc attagctaaa tattagagtt
     1201 agagccgaaa acttgtcttg acagattcat cacctctgct ccctgatccc ggcatattac
     1261 acactaatcc ctctaaaaac cagaaacaag tgccggaata ttcagtttat ctctacagga
     1321 gtgtgtcaat ttaagatttc cttttattta aagttttcct gattttttaa aagttgtgtt
     1381 gttcttgtct ttttttattt tgcatccaaa cttggaggtt tgtttagaag agagatccag
     1441 gttggcaggt cactaatact
//
LOCUS       KCVB01000702            1223 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33333pri ultra-conserved element locus
            uce-1074, sequence.
ACCESSION   KCVB01000702
VERSION     KCVB01000702.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33333pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1223
                     /note="ultra-conserved element locus uce-1074"
BASE COUNT          360 a          251 c          264 g          348 t
ORIGIN      
        1 ataggacaaa tctgtagtca taaagaaaaa aaaaatgttg cccagaaaaa ggcactgacc
       61 tcgcatatat tgattgctgt tcaacgagag cctcacgcac agcataataa caaccacggt
      121 gagcacggta gcgttgatat ttgttgtttt ttttatgttt cctatttgtc ctcaggttta
      181 gttttaatgg ctgagcagac caaggagaga aacacaacac gatattctct aaccataaat
      241 tagatttgca cctccagcca actttcgctt tgctacttgg taacttgtct cagttctcat
      301 aagtttttga gtctccagac gcccccctcc cctccccgac tctcatcctt ctggtcagtg
      361 aaactggtcc cagctgcact taaacagaaa cccagagctg cggacgtaga cacattcaca
      421 cactcccact ccctcaccga ttccctctga ggcacatagg gttagagtaa tatccaaagt
      481 gcaggtggga ccacactttt cgtagcgaag ctgaacaaag cagggtgaga acatgagcca
      541 agtaatggag tgatgatcac tgcagagatt gaacaatcaa agtggggagg ccggtggtgg
      601 gggcactcat ggacttaggg aacttgcaca ctaattgtat cttatgacta attggtaaat
      661 tagtgaaacc tatttcaagg cgccaggctt agctttgggg gacacccaca gctcattata
      721 gactcatatt ctgggcagag gaggagaaaa ataaaggaaa ttatacaatt gctcaggtga
      781 gaatggcaat taagcaagtg cttaagaaga ggaggaaatg aagcctcata cacgcataat
      841 ttactatact ctcttgtgtc aagctttaaa tcaccagcga tctagggcac atatttggca
      901 tggagaggga acaagcattg taagtgtttt ttgtttacgt acttatttgc aacacctgta
      961 tgtgtatgtt tgtgtgcatg caaacatgcg tgtgtatgtt ttcctatggt gtatgcacta
     1021 caagttttaa tcaattacta aaatgctaat tattgaattc tttaataatg tgactgcaag
     1081 gtccataaac agtatgtctg ctctcccagt tgagtcctaa taaaccttcc ctgaacagta
     1141 tttttactta ttagtgttat gattttggac agttcaggcc tgattctgtg aagaagcatt
     1201 ttgcctgagg aggttagaca atg
//
LOCUS       KCVB01000703            1410 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33371pri ultra-conserved element locus
            uce-497, sequence.
ACCESSION   KCVB01000703
VERSION     KCVB01000703.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33371pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1410
                     /note="ultra-conserved element locus uce-497"
BASE COUNT          432 a          248 c          284 g          446 t
ORIGIN      
        1 ccgttagcca cgctttatag ccattattta gatagcgaca caacgggatg ctccttttgc
       61 tctttgactg acatgcccag ccatgtgctc agcaattcag ttctctgcat cggacaaagt
      121 gcctacaagt ttgtgagtgc acaaaaaatg atgatgtgtc agtttgtatg aaggagaggt
      181 tggacagggt tactgttgat ttgataggaa tacacaagta gcgagccgta attttacctt
      241 ctgtcagtgt agaaggcaaa gtttgttaag tgtgttactg ctcttgcacc actctttgtt
      301 atgcattgtg cagcatttgc agtttattta ttttacaact gttggttata taggagaaaa
      361 aaacagacag attcttgagt ttgtttttcc aaaattataa aatgcgtaca agcagtatat
      421 tgtgctgtat gttcagatta ctggttatat tttgggatat ttatcatact atttacctgt
      481 aatgccacaa aacatgcaaa tcatgaaacc ggatactttc cccttatgaa attatgacaa
      541 accattcaat ttggacaaca aatcggctcc aactactttt gtggcgcctt atcaggaaaa
      601 gaacagcaca gaaaaagtgc tgcacagaaa aaacattttt ctttctttgc caggaaaaat
      661 gaactgtcaa catttaatga tagatatttg cattgtgata caaatcgtgt ccttggccct
      721 aaacataaga tatagatcca tttataatga attcagataa agtgtccttt ttgcattctc
      781 gctctaatgg atttgtggag ccagcgtcag tgaccctggt gcaatcaaca aaaacctgtg
      841 ccagtggatg atcagacaga catctatcat ttcctctcac accttctaag acattgctga
      901 tacttaggtg caaatatatt ttttcagcca aatgtatgtg taacttctgc aagctgactg
      961 actttttttc atggttttgt gagaatgtgt ggaagaaagc gggcaaaaag acattagatt
     1021 ttttttccat cattaagctg tgtctgggag atgatgtgct agccgtatgt ggtccagggg
     1081 aagtatttat agccctgctg gaatcttgct gtgctgcgtt gatagcagag ataatgggag
     1141 agaatgggac gcagcgaact gttctaatga caccaacaag acaagagaac agcgaggata
     1201 aataaatgtt agccattttc tctcgcacat catttgcatt ttttcttgtt aaggtcactt
     1261 atagactcag acaataacag tgagggaaaa aaagctcttg cagaatataa aatcagacat
     1321 aacaaaaaca gaactattta gtatgcaagt cattgatttt atttcctaat aatgtgatat
     1381 tcagttaaag atagtgccgg tttttaattt
//
LOCUS       KCVB01000704            1308 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33415pri ultra-conserved element locus
            uce-805, sequence.
ACCESSION   KCVB01000704
VERSION     KCVB01000704.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33415pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1308
                     /note="ultra-conserved element locus uce-805"
BASE COUNT          416 a          224 c          262 g          406 t
ORIGIN      
        1 caatttgtct gtcaagctgg ctgagatgtg ggggagcagg aaaaaagcaa tttagcttgt
       61 gaggtcctca atagaaatgt actacaggag cctgtgtatg acatgttgca ttagaacctg
      121 agtagccttt attgcctgat aatgtcgcaa ggcaatgctg atatcaatca ccctttaaca
      181 gccagtatga tgtgttttta tgagaagaaa agaaaaaata atgcatgaag catttctcga
      241 aaaataattg atttaattct gagctgaggg atttctactt ttaacaacat aaaaatattg
      301 tgttgaaaaa aaaacaggca caaaatgtgc tagaagctaa aaattgttag gaacaagctc
      361 cttgcatatg ctcctataga aaattgtaat taattttaca cggataaaca ttctgtctca
      421 tttgcatata gatataaaca atatatttgt gcatctgaat tcccagcaca cttgggtcac
      481 gtggtctgcc taaaatccta agtggctaaa acccaaaatt atcattatat ttcccaggcc
      541 ataaatccgc cttttttctc tgacacaaag ccttataaat ctgtgtctga tttttcacat
      601 tgcacattgt ttttagttaa tgattttcat ctgtttgttg ttgctgtgat agtggggaaa
      661 tgtaccatat gctccggcaa aagacaaatg attagcaaat gaaattctgg acagctgtct
      721 gactgaaagc gtttggttga tagcattctg ctaccataat tagcttaagc tttgggttaa
      781 ttaactttct acctggatat gcataataat agcattctgc agaaagccaa ggagcttcca
      841 gtatatcagc agcaaaggct ggctttttat tttgtatttc ggctgcatat ttcaatttca
      901 agaggcgatt tagctcggga gcatcgtcaa atattgtaga ttttactccc caagccagat
      961 aattctgaaa caataggggt gtattatgtg gcagaaatgc catttctgaa tgtaattaaa
     1021 gcggatgaaa gcaatcacca atttttaaac gcttttgtct agcatgaggc ctacttgtaa
     1081 aaaaaatacc catcaagagg agaaaggagg caagagaacc cccaagtcct tatataagag
     1141 gcctttgaga ctgaagtgaa gtacaacagt aattagtctt ttgtatgaat ttgctggcca
     1201 tggtcttgat tagttaagca tatgagagga ctgcaggact gcattggtct taaggaggag
     1261 ggtgttaaag tctatttatc accagttatc ctcacagctt tccattat
//
LOCUS       KCVB01000705            1436 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33493pri ultra-conserved element locus
            uce-840, sequence.
ACCESSION   KCVB01000705
VERSION     KCVB01000705.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33493pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1436
                     /note="ultra-conserved element locus uce-840"
BASE COUNT          389 a          365 c          306 g          376 t
ORIGIN      
        1 taagcctttt aggaaatccc aactagaggg taatgtgtaa tattagtgaa ctatgtttta
       61 cagaatagtc tcaaaaatag gttttccaca gcatggattt atcataaatg gtttgcatga
      121 attcaggctt tatcatgcac aagaattatg gtgaatgtgt gggaaatgag agttgacacg
      181 gttgcagggt ttcccctttt actggcactc gtgcacatta cactcccaga tttctagcaa
      241 atgcatgtat gtgttacctg acataactga caaaatgttg cttgacatac ttcactttta
      301 cagatggcac aggaatgaca gagaaagaga gagagagaga gagagagaga gagagggatg
      361 tgaggagaaa gagaggggaa gggagagaga ctctaaaagc ttgtcagtcc ctcttaatta
      421 ttctaattgc tgagctcaag cttcatgcca aggcttaggc attcccctgg cacaacaaca
      481 caagccccca atcacacaca cacacacaca cacgattcag ccactgcatt gccttgaaac
      541 ctctcctcgc tcctcgtctc gtcctctccc ctcatctcca tacagaactc ccccatctcc
      601 ctcttttcct cagctccctc tatcagcatc cctccaccct cagagcaccc tcttattcct
      661 ccactccgcc tcctctcctc ccatcacaac agacccttct gcttcctctc cccagtcact
      721 cctctcctcc acccctccgt tcctccgaac tggattaaaa gtacttttca ttacccgtgc
      781 gggagatatg gcacttaaaa atcaccccat tatcattttt cacccagcaa acagcacaca
      841 ctttcacaga gacataatta ccacaccctg tcataccagg gagatggagg ctgctgattt
      901 ccagccaggc cgtgtgtgtc actttgtgtg cgcaagtgtg tgtgtgtgtg tgtgtgtacg
      961 tctgtgtgta taagaaactg ctgcctgtcc gcctgcctgc ctgcttctct tattgatatt
     1021 aaaagcgctg cataaacgag tgtttaaaat ggctgtcaga gggggagaga gagaggatcc
     1081 actccaatca caaactgcag cacacatcac tggatctctt tcccagcttg gcacatggta
     1141 atggcctgga ataacagaat actgctggat gattcccatt cccacgttca aagttgtgag
     1201 aggctgcaga ggcagttgaa atgggagggg acgagtgata gagctcgctt taaccagtca
     1261 acacttaaca cataatgaat tgggagtcat atgatgaatt ggatttatgg caatgcttgt
     1321 tcttaatggc cgaagtgctt caaattggga ggctggggag gagaaggggg accagactca
     1381 ttcacactaa tattcattta ggaagccggt cctcatctaa cagttacacc agttct
//
LOCUS       KCVB01000706            1223 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33506pri ultra-conserved element locus
            uce-1089, sequence.
ACCESSION   KCVB01000706
VERSION     KCVB01000706.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33506pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1223
                     /note="ultra-conserved element locus uce-1089"
BASE COUNT          351 a          265 c          252 g          355 t
ORIGIN      
        1 agagagagag agagagagag agatttaagc tggcgactgt atgaaactgg atggtcctcc
       61 gagtacagcg tcaacagtta cattcagcaa taattttagt acgtggattt aaaagatttg
      121 aggctttttg cacataatac ctccccctca gtcgttcctc tgcatatttc cacctcgctc
      181 tgtatctctc atagctgtac tgaagttgtt gacttgctcc tcatgcgcgc tcctcatctc
      241 ctcctctatg tgaatcaaca gacatggtac cagtgagagt ctgactgact ttatcacatg
      301 gtacacagct tgttaataac ttcctctgac aattcctcct catctttgct gatgaccaga
      361 gggacagccg tgattgtcat gtttgtaggg gaagaaagac tctaaacaat gatgtcacgt
      421 cagcctctct attaaaagac ttcatatttt gagcggcata attaatactc ttgggcacgt
      481 cgcttccaag ttggcactcc aggcaaaggg ctgccactca cctgataatt atttttggaa
      541 atggagccca aattacagtg attgacaaat gcaacaaagc agagacagcc tcaagcattc
      601 atgaagaaag aaatgagaaa aacagttaag tggttaatgc actttcacag tggatttaaa
      661 gtttaaatcg cttgtcctca catgtctcaa atgtaacttt ggcgagggtc aaacaattta
      721 gcccctgtat cctcttgaag aaacacccta aaaggccttt gaaggagtaa ttaacacttt
      781 tctcatttct gcggtaaaaa tgtgagaggt ttttattact tttttaatag tagtcgtatg
      841 ctcagatatt ttcagggcaa catactgcag ttagccaact gttatggtgt cagaaagctc
      901 ttcacaaatc acagtcagca attcatatga atccaagtgt gtcctcactt cacagttgtc
      961 tgcaaaaagg caaacagttg ttagcccgtc gggttgcatc cattagatgg tggatagaga
     1021 aaagcggcat gcctccatgc tttttacacc tctcgccatg cgtaaatcag ccactctgtc
     1081 attctactct gaagagaggg ggaggaatta tgtcaagtta tttgttttgt accaaaatga
     1141 aatatttatc gggagtgaac acctaccagg aacagacgat cggccagggt gcttcatctt
     1201 ctcagactca tatttggctt cca
//
LOCUS       KCVB01000707            1437 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33542pri ultra-conserved element locus
            uce-796, sequence.
ACCESSION   KCVB01000707
VERSION     KCVB01000707.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33542pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1437
                     /note="ultra-conserved element locus uce-796"
BASE COUNT          458 a          261 c          235 g          483 t
ORIGIN      
        1 atgccaatat tcatcggcag ttgaatcaag ccacttagat accatctgta aacaggactc
       61 agagggcttt gaattaaacc aagggctgga aacagtggag ctcactgcag agattactaa
      121 aggttcaaca caggttaatg ggctttgttg ccatagatca gttgtataga tattggcagg
      181 aatctgctga aaagcttcca tctcctgtat ttctctgtta ttgcaaatta ttgttgccat
      241 tgtttttaat ttgaaaatca tgctacacaa tcttaaaagc ctaatcatta cttaaagtaa
      301 atgaaggtac cagtatcagt ggctaaatct attagtttta cttacagtgc ttagtttagt
      361 ttcaattaca ccataattca aagtcaaatt cctgatttac atggtggtat gccttaattc
      421 tgtttacatg agtcatgtca aaatttccta ttctacagaa acactgttac atatcgatat
      481 atgttttgtt ttactgataa ataatgtttt ttcaaagaat tctaaatttt aaaataaaaa
      541 tgtaccattc ccaatttgcc gccgtattta acataatata actactttgt gtacgatttt
      601 gatgcaacca atgagtattt ttccctcatc ccctcttcct ctctccgtct gtctccctac
      661 tctctttcgc caccatggcg ctctgctcac caacactgat tcataatcta tagccgtgcc
      721 tgggagacca gcagctattg atttaacgcc tacagtaatc cagcgctgta tccgctgctt
      781 gttagagatg cttgggtcta gaaactgggg cttgcagagg tagccagaga gggactagga
      841 ctgatggagg aaacaatttt aagagcgatt ttttatttaa aaaagcaggg aattttggaa
      901 atgaccctct aaatgagaca ttcttaatgg ctctaaaata caacaaaata tataatgata
      961 gaaaaatgta cagtgcagag taataccagg tatgaacata aagaaaaaga gttgcatggt
     1021 cacatttttt cccacttggt aacagtaaat caaaatatgc atttttattg tgactattga
     1081 ttaagaatct gatacagcag ttttccacat cagaataatt cagtgtaatt taataaatgt
     1141 catatctaaa tcatatatca gttacacttt tagctgctgt gtgattaatt aacatactgt
     1201 gtttacagag ctatgactaa atttgatgtc tcttaaaaca ttattacatt attattcatg
     1261 taatttgttt ctacaaatat aaacaatttt aaataattgt attacatttt agcaatcatt
     1321 caacaagggg ttgtacatct ttcaacatga tgaacactgt atctttgaat caaacttttc
     1381 acttagaatt gtccttctct gaatggctga cactgacaga gtcaagacct cctctgt
//
LOCUS       KCVB01000708            1568 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33664pri ultra-conserved element locus
            uce-577, sequence.
ACCESSION   KCVB01000708
VERSION     KCVB01000708.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33664pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1568
                     /note="ultra-conserved element locus uce-577"
BASE COUNT          468 a          291 c          482 g          327 t
ORIGIN      
        1 ggtgagggag ctggtggtgg tggtggtggt ggtggtggtg gtggcggggt ttaggggggt
       61 gagaggggtt ggtgtggtga gtgagggagc gaggggaaga agaaagagca aaagcgacag
      121 agtatattat ttcattaagt gctttggagc tcggcaggga ttggggagta aaaccttgca
      181 gctctgtgtg tgacaagggc acgacctccc gcaacctggg aactctatcc acacatcgcg
      241 ccgctttcat ctgatactgg gggtgtgtga gagctctcag tcactggcac agggctggcc
      301 tgctaaagtc cactaagctt tttagtcagg gcccgctgaa cggaggaaaa ggtggcgaga
      361 aagatggagg ggagggatgg aggacgaggg agggaaggta gagtgattgg ggttgggggg
      421 gcagggtagg tgaggagagg agagggtggg ggggggttcc cgagccacgg taagacagaa
      481 ttgggcattg tcgccaagca gagcagagca gagctagccc attctgttga tctgagcagg
      541 atcccggaga aaacaaaact gcattgttcc ccgcagatgg aagccagtgt ctcctgggta
      601 acagcattaa tctccccttt tctcctgctt cctctcttgt cggggccata atcggtgggc
      661 tcctttcagc agccgagcca gcctcaaagc ccaggctcct aactagcctc ctaattagct
      721 gcaattacaa tgaagacaac atgatgagat aatagaaatt tgcaaaataa tgacatacca
      781 cgggaaaata ttcctcctcc ccaagcacca tttttccctc cgggagaggc acaatggaga
      841 gccggggccc ttttctgaaa atggggagct cagaaaaaat ggtggcgcat tatgtagcat
      901 tgaaccctat tgtctgcctt ctggttgctt caatcaacag gccattgtct ggctgcttaa
      961 atcaaaatga ctcaaataca ggctccgtaa tgatgccctc tcagcacagc atactgaaca
     1021 gagagacaga ttgagggaga gagaggggag agaagaaaac aagaggaaag aggagaggag
     1081 attagaaagc aagaggaaag agcagagaag caaatcatat gaaatgtaac aaaaggtaaa
     1141 ataattatga gaaggttagg aagcggtcct atcgtccgat atcctgacat gagccttacg
     1201 tatcagcagg aaataatgga aagcatctga atacgactga tgaatcgttg ctgctgcaca
     1261 gttgatttca tctggtctcg ttagatcaca taaaataact gtaaataaag aacaagaaga
     1321 cgagacttta tgcaatcaaa aaagagcgag gcagtcagag aaagggagac agactgagaa
     1381 aaggagggag gggaggaaga tgaagggagg agacggaggt agacagcagg tggtagtcat
     1441 gcacagagca acatttttta agcaactctg atcggcaaca agggactaag agctaccttc
     1501 tctacgtgag ttccgttagg atggaaagag taatagttgt tctgatgact aaaacactag
     1561 tgaactta
//
LOCUS       KCVB01000709            1445 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33704pri ultra-conserved element locus
            uce-1194, sequence.
ACCESSION   KCVB01000709
VERSION     KCVB01000709.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33704pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1445
                     /note="ultra-conserved element locus uce-1194"
BASE COUNT          397 a          374 c          253 g          421 t
ORIGIN      
        1 acacacacac acacacacac acacctgtac agtttgtgtg gatgaagctg ctgctgctct
       61 caggttggtt ttgccaaaat aaaagtcctt caagttatgg atcaactttg ccagctgtgt
      121 gcagctgttt gtgttcggag catgtttgtt cctccagatc gtcctcggcc cgatctgagg
      181 cagggaccca tcccccctcc tcatcctcct cttcaccctc ctcctgcatg attcacctcc
      241 atccgatcac tcacctcctc tgtccgtctg cctgctcgtt accgtcacct cctcctgtag
      301 gaccgcccct tcctccctca cctcctcctc ctcctcctct ctcttcctct gctcccatag
      361 gcccttattc attctctcct gtccatatgg tttctgcagt caaacagaag agcgccttcg
      421 cccctgtcgt gcggccacag acctccccgc cccccacctg caccaccacc aatggcagca
      481 acctccaggg tgagcatccc accccccctc ccgcctgtca gcacccccgt cctctgctgt
      541 ccacctgtcc atgtcccacc tggacacgca gacagacatc cagcagtgat gataataatg
      601 tgtttgttac atgatttgaa ctcggaggtc aaagttgatc cataacttct ggcacagaac
      661 agaaaaacaa gcctttcttc atcttatcca gtaattttgt tttgctactg agaattcagc
      721 ctgtttcact cacaaaaatc aggtgagact gttcatgatg cagtgaagca tcctgctgca
      781 aacaggttta aaggcaaaag tgttgtttct ttgtgaaaaa gtccaaataa aaaagacatg
      841 aaaatttgtg agtgaagtaa atgaccaata atccatcaca gcacagacaa acgagctttt
      901 tgtttcggct taaaactgat aaaatataca aattatcgtg ttaataaact ctaaaagtct
      961 ttttttcagc agacagtaaa gaataatcgg caacatgttt atgtaataaa aattaaatcg
     1021 tttaactttt attagaacaa aagtgtattt aattgaattg ttcattctgg tgcagcagtc
     1081 tgcattattc ctctgtgttt acttggcgtc tgtagataca aaaaacaaac tatttgtttc
     1141 aggtagtttt gaatataaag ttggtttcca tgacaacact gtgatttctg ggtcatgagg
     1201 agaaacacag attgttgaga acagcaatga acttactgag actgaagttc ttaaagttta
     1261 ttttctgtct attttcactc attttaaaca agaatgtaac ttcatgtttc tgagttaatt
     1321 cactgatggt ggaaaacaat gaaacaacat gaagaaaaca cactcagacg cgttgagagc
     1381 aaacagtgtt tcatttttcc tggaggaaga aaaaccacaa acacactgat tctttcactt
     1441 gttta
//
LOCUS       KCVB01000710            1078 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33742pri ultra-conserved element locus
            uce-1169, sequence.
ACCESSION   KCVB01000710
VERSION     KCVB01000710.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33742pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1078
                     /note="ultra-conserved element locus uce-1169"
BASE COUNT          315 a          221 c          195 g          347 t
ORIGIN      
        1 agctgtccat tctagtaact cttaagggaa acacctcatc cttatcagac tgattattat
       61 gacatttaga gagtgaatct gcaagaaatg caaaaaataa agtttcagtg tattagtttt
      121 tttgctgtag aaaaacttca ttttctgcaa ataaattcat attccttcag ctagttttta
      181 cctttaaatt tcattctcgt aactggtctc ttgtgccttc ctactgcaac caggccacat
      241 tgtctagagc catgaactca ataaaaataa acaaaatcaa ttgtcctttt tgccttcttt
      301 atgttatcta tttactttgt tttagatagt aatctggttt ctctcttgct gtccgtgctg
      361 taggtaggtt gatgcgttgc ctgcgctgcc ctgttgcgta ccacgtcggt gacctgtgtg
      421 tggctgctgg cagtgagatg atcaccaaca ccgccatcat ctgcaccaac cacttcaatg
      481 ccaagaaggc ttacagccac cacagtcatg ttaacgtcag ctggtgcttc gtctgctcca
      541 aaggtttgcc ccttctcctc tttaaccaac aaggagggca gttactaata gccaaacatt
      601 aatgattggt tgtctactta gccagccagg taacctcact cattttaagg atctttgtgc
      661 tgtaacaacc aaattttagc tgtaaaacag caagttttga cttccattta acaggtaaaa
      721 cgtgagtcaa tttcagcagc cacagtcata gatgaaaact atttagtacg ttactttaat
      781 ttagttgatg cttttgtcaa aagtgacact tacaaaaagg cattcaacca cgtggaatca
      841 tgcaagtaca tcaacttcaa ataagagaaa gttttgcaga gacggctgta ttgtcgtctg
      901 ggagtcattt tctaaagttc tgtgaagtga tactaaaaac acagaaagct aaaaggttaa
      961 tgctaaatga cttttattag tatcaaaaat gattaactta atgattaaaa gttgtgtctt
     1021 taatgtaaaa ttcggtggaa atgcctctga gcctctgcag cagtctgtct gtatccac
//
LOCUS       KCVB01000711            1226 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33807pri ultra-conserved element locus
            uce-918, sequence.
ACCESSION   KCVB01000711
VERSION     KCVB01000711.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33807pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1226
                     /note="ultra-conserved element locus uce-918"
BASE COUNT          354 a          276 c          260 g          336 t
ORIGIN      
        1 tgactgtaat taaactattt taaagtgagt aatttgatca tttttgagtt gggaggatct
       61 taccacataa acacccactt aaactcacga aatctgaatg aatatagcca aacagtccat
      121 gactgacaaa ctgcttgttt ggatgaaagc tgcacacagc aacattaatg gacagacaac
      181 cagctgagga ggctacagaa ttaggatgtt agtgatttgg gtcaatttgc gtttgctctt
      241 ttgagctgac caatcatagg ccttaatcac tgaaaggtcg ccttgaatga caccttatta
      301 gggcggggca gagtacgtgt gtcagccatg gcaatctctc cgcatttcag cctaaggcag
      361 tgggtcagcc agcctcaggg gtgggtgcta atggatgcaa gtcattaaca tcctctttac
      421 aatccccttc acacactctg tcccttgtgt ttcagcgctt ggtgccaccc aaccctccca
      481 tgtacttcca atgagccaaa gcgactctgc ccccttcttc caaattacaa actgtgcgtt
      541 tgctagcgcg ctcactgcca ccccttaaag ctgcttaatt atggtcattg gcaccaagtc
      601 tgaaagctcc ctttatcccc cccacgctcc agaaataggc cctgctgaag cacacaggca
      661 acccagatca aacgtttccc atagggaagg ggtcattact ggtacgcagg gcggacacaa
      721 cccaatgggt gctgggccac gccttgagcc caaattgcag cctgggccta gtgaggacac
      781 aagactgttc tttgatgcac ggcaagagta acagcaacac aagctgtttg gatatcagtg
      841 tttacagtga gctgtgtaat gtacatacac ataaacaacc aatacagtgt tttgcggact
      901 aaaagcaaag aaaattgggt cctgggcttg gccgggtgtt cacttgtttc tcatggttta
      961 tatttatctg aatgtcacag ctttttttct caagtagctt gtttagattt ttccccagtg
     1021 actaagactt taaagtagta aagattactg aaattctgca taaaatataa ggattttaat
     1081 aatattatta caatttttaa cacctaagga cagatgctaa ctaagtagca taaacagtga
     1141 tgtttttaga gcacatgtag atgtaaaata acctcatgtc accaagggaa taaataggtt
     1201 gtaaagacag tcacttcagt gtaggt
//
LOCUS       KCVB01000712            1054 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33854pri ultra-conserved element locus
            uce-1295, sequence.
ACCESSION   KCVB01000712
VERSION     KCVB01000712.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33854pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1054
                     /note="ultra-conserved element locus uce-1295"
BASE COUNT          323 a          200 c          282 g          249 t
ORIGIN      
        1 tggtgtcttt tttttttttt ttttggaaga aaacgggtgg ggggacatta gggagaagca
       61 tgagagagta aaagtgtcaa cgttaggtga gaagaagagt ggaggaggaa gatgttagaa
      121 gaacagaaga aataaaagtc tcatgcacaa gaggagaaca cgcactcaca ccccacagtg
      181 gcagaagcgg tgttggcggc agtggcagta gtggtagtag tagaaaaggc tacatgtgta
      241 gggctagaaa cattggttac atcgtgctgt tgttggctgg agttggaggc ctgccgcctt
      301 agagccccct gaaaggaagt tccactctgg aacgcactca ctacatccat ctgcaaagaa
      361 tcagacagcg tgacaggaga aagcccagag gaaataaaac aacacagcca gaggagagag
      421 aaaagagaga aaaaataaag aaaaggcacc agcaaccata gaaaaaatgt cacttggaaa
      481 ttggctttag gcattatagt taaaagagag aggaaaaagg ggatagttgg gtttaaaagc
      541 actgatcgca gggagctcgg accccaaaat ctgacctcac tttcactctt ttctctgcag
      601 aatccttcaa tacttccagc tatagccatg ccaatactct ttgaacattc agctgttcct
      661 agcgtgctct ccatccaatc caggttactt gcttctcatc agtaccttca aactggtcgg
      721 gttttagcag agagattcag accgagtcag agataggcat aggaacccgc tttagctcag
      781 aagcagaaca gaaaaattag agggaaggct tttgttttta gggcacaagc cattctttaa
      841 gagacagaac aaaacatata tttttaagct ggaagagcaa tagagagtta aagggtttat
      901 ggcttttgat tcccttggtg tcgggtgctg tgttcggtgg tacatgggaa ggtctgtaga
      961 ttgttacctg agtctggatg cgattgaggc ctcggaacca gaggatctgg cctcggcgaa
     1021 gctcccgttc agcgtggtca atctcatcca tgtc
//
LOCUS       KCVB01000713            1379 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33900pri ultra-conserved element locus
            uce-363, sequence.
ACCESSION   KCVB01000713
VERSION     KCVB01000713.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33900pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1379
                     /note="ultra-conserved element locus uce-363"
BASE COUNT          370 a          337 c          308 g          364 t
ORIGIN      
        1 attggacggt ttacagattc acatttgcct tgacagctct gtatttctta atgcgcaata
       61 ttttgttcca tgataaacaa taaattgact acagaccaga gattccagat taaattgttt
      121 tctatttatt attacattca ttaaccaaac taaacctcag ccgcaacata tgcgtccacc
      181 gagtaaaccg agagaaacac agagtgcgag aacaatgcat cagaagttca gctgaaagaa
      241 atttaattgg aggtatgtat atatagaaat tttattttcc atgtaccaac tgtgacatgt
      301 atcattaaaa tctttggcag tcctcggtgt cggccaaatt tcacaggttg ttttcgtttg
      361 cccagttctt gaagtatcca aactcccctt taagagtatg ggtctatgtg atttaagcca
      421 atgataggac tctatatacc gcaggaaaaa actgtgacat gtgaggcagt gggtctttaa
      481 agggctgctc tgcccctact cacaagccca atctcgtagg acttgttagc gtatcacgtg
      541 ccagattctt aatgaagtgg acacacgagg gcctcgtgtg cgcatgcgct tggtgtaaaa
      601 tgtagttgga aatgaggtgt ccgtcttgga gtagtcgact tttaaaaagc atcggcagcc
      661 cctgatatga cgacttcgct ggttctgcat ccacgctggg cggacacctt gatgtacgtt
      721 tatgaaaaaa gcccgaatga aaacaaccag aataaaagcc aaacaatgga gggactaagc
      781 ggtaattgcc ctgcgaccca ctgcagggac ctgatgtcgc accccgcgct gggaagacat
      841 tctggcacca tagcgaccca ccagggctcc gtctattcgg atatttcctc tccagacacg
      901 ggccggcagt gtcccgctcc tcaaacatca tccagtgcat ctttgagcta cggttatccc
      961 tttggaaacc catattacgg gtgtagatta tctcattcgc acaacgtgaa cttgcagcag
     1021 aagccttgct catatcatcc cgcagagaaa tatgccgagc ccagcacagc gctccccacg
     1081 gaagaactgt cctccagggc gaaagagttt gccttctacc cgagtttcgc cagttcatat
     1141 caggctgttc ctggatatct cgacgtgtcg gtggtgccca gtatcagtgc ccatcctgaa
     1201 ccgcggcacg acgcattgat tcccatggag ggctaccagc actgggctct ctccaatggc
     1261 tgggatgggc aggtgtactg ctccaaggag caaacgcagt caactcatct ttggaaatca
     1321 ccttttccag gtaaggaaaa tttatgtata tgattttttt ttttaaagct gcccccaac
//
LOCUS       KCVB01000714            1423 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33943pri ultra-conserved element locus
            uce-899, sequence.
ACCESSION   KCVB01000714
VERSION     KCVB01000714.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33943pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1423
                     /note="ultra-conserved element locus uce-899"
BASE COUNT          428 a          265 c          296 g          434 t
ORIGIN      
        1 tgtaatgaat taacatgtca tagtattaag acaacaatga aactctctgc ttagagaaat
       61 aagatacata tatcagcctt caattcaaga taaattcatc ctggcgctaa tactgtcagg
      121 attaacagtt caattgccac agagggaaaa atcataaatg catagtatat gcatgaactt
      181 caatgtgcac caagtgacaa atatattaaa ttcagcagtg tcattgtatt aaagatactg
      241 tttgcttgag ataagatatg aagaaaaaaa atgaaccgag ggagaactga gaaaaaatta
      301 ttaggaggag gatacttaaa gaaagaagag aaaagggaag aagaaggcag caaagtgctg
      361 agaggaatgg agagagagat cacaggtccc tcttgattca tcctgattac aaggttaggt
      421 taaggttaag tgacatgggc aagtcgtgcc tctcctcttt ctctttctcc ctctctttgt
      481 ctccccctct cctcgtttaa aggtgagcag ggactcttat tggttatgcg gccctgcctg
      541 aagttatgtg tggctattgt caagagcgaa gaccttttta ttttttacac acagctgctt
      601 actgctaaat agtgccttgt ccagaagggg acaatctggg cctctaattg ctgtcactga
      661 agcctagatt cattatgtga ctttcccatt aaaatgtgag cattaatttg tataatgaaa
      721 tatcaccctc tgcagtgtgt tcattagctg tggccccaca ctgtagcacc acagccgcac
      781 aatgttagct acctagaaaa ctgtggttaa ttttgctaat ggatattttc ccactcactg
      841 ttcaagagga aattaatact gtaacattag ctctttaact tttggagaaa tttaaattca
      901 cttcattcct ttcaagctgg tgttagccgc tcattaatct ctgcgttgca ttgcacaatg
      961 ctgcctagac caaaactcca ttgtgtcact cacattgcat taggatgaag gggagcttgg
     1021 ctgtcatctg tgaatttctg tgcattgtca ccagagcaca gagaggatct ttagccactg
     1081 taaaagtcat ctcgtggatg taatcaggat gtcacagtgg agctggtatg attaggttat
     1141 aggaatgatt taaaggccac atgttgagga tgcatcaagc tgtttttctc tccacgtgca
     1201 ggagactatg ctaaagctgt gcttagttag gcccacatcc agagatacat tccattctaa
     1261 aaagacaaca tccttaccaa cctttcctca cttttgctcc ttggtgttga gggctcaaga
     1321 ttaagcctat tctttaattg tatttttaag aaagttatga atggaaacat ttaaaatata
     1381 ctgtgtggat gaaagtagat aggcttattt gtttgtgtgt gtt
//
LOCUS       KCVB01000715            1507 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 33986pri ultra-conserved element locus
            uce-66, sequence.
ACCESSION   KCVB01000715
VERSION     KCVB01000715.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="33986pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1507
                     /note="ultra-conserved element locus uce-66"
BASE COUNT          507 a          290 c          332 g          378 t
ORIGIN      
        1 attgaccact ttacaataca gttagaaaat gcagcatatc aggacaataa gtctttcttt
       61 cttccatcat tgtccctgat gttttcaaag tctgtcaacc ttcattaaaa tgggcgacaa
      121 tcatttaatg tctgacatca cgatatatta gaaaaatgaa ctaggcaaac ctcaaaaagt
      181 gaatgaatta aagacgcttc tggcacagga atttttgaga accttgtttt cttttgatga
      241 taaaacaaag tatcaaaata tcattgtttt gtcatcagaa caaagagtca gttattgtat
      301 taataactat ttaaaaaaat ctcaaatgat accgagccac gtgtgagtaa cagcattttg
      361 tgagtcctca ggtgtgtttt agtgtgtgtg tgtcctctca ccgtctccct gagctccctg
      421 ctgcagcagg aagctctggc cctcgttcca ctgcctctcc aggagctgct ctatgctggt
      481 ggccaccggg ggcaccgtct ctccgccgcc tgcccccgca cctagcaacc ccagccccgg
      541 ccccggcaac gaccccgagg agtcatagcg gatctgcagg ccaccaatgg gagagctgga
      601 cgtagggaga gagaaagaga gagagagagg gaagagagtg agtgagcatg ggcagaaaag
      661 gagagataga gaggtcaatg gggagattaa cgacccaaca taaacgtcag acaaacatag
      721 acactgctgg ctataaaact acatcaattt gttgttacag tggcctctaa tgaaaagggg
      781 ccaggctttc tgtgtgtgtg tgtacgtgtg agtgagtgtg tgagagagag agagagagag
      841 agagagagaa agaggaagta agaggacatg caagagagac agacagaggg atgtgaactg
      901 tcttgatgtg ataaatcttg gtgtgtgtgc tcagataatc tgttgagtat gaaagacggg
      961 aaatgaggga cagcaggaga gagtgagaaa gacagaaacg agaggtgctt aacgtgggga
     1021 aaaagtggaa aaatcctgtc attctgtccg tttttcgctt ttaaatcaca ccgcaaaggg
     1081 aaatacaccc tgattttgaa tgtcaatgtc acagcatttt ccaaaaaatt taacttgggg
     1141 tgcaaaaact gcaaaaatct tcaaaatatt taaaatgaca ttaaacgtac aacttcaaca
     1201 ttttctccat cagaatattt tctttttatt caaatgccac cctgtccaga attatgttta
     1261 ctaatatgaa atacatgaaa tattacaggc acagtttaaa aattaaatgt tacaaaaatt
     1321 tataaaatga gtgaccaacc tcatcaagta aaattatcca tcccacaaag aagtggaaac
     1381 aaacaaaaat ggaattatac catcaatatt cccatcctga aagatccatg caaatgttcc
     1441 caaatgaagg agatgcagaa aatcatcaga ctgttctcct tccatgtgtg attaaaccca
     1501 gaaaaaa
//
LOCUS       KCVB01000716            1629 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34055pri ultra-conserved element locus
            uce-864, sequence.
ACCESSION   KCVB01000716
VERSION     KCVB01000716.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34055pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1629
                     /note="ultra-conserved element locus uce-864"
BASE COUNT          464 a          320 c          405 g          440 t
ORIGIN      
        1 aagcatggct tctgatatga tactacatga tgcctatgga tgtgctacgt cctttgcaga
       61 aaactgatat tgacattgat gtgcatgtgg cacattttcc acaggggata agcaaacaca
      121 cttgtcacgc tgtcgttgta atatatattc acctctgatt catttttctg tcaattatgc
      181 ttccttttga gtgcattgta tgcactgaca gagtgtgaaa attcagctta atgtcacgtc
      241 tgcctgtgcc tttcaaagag tggtaaacac tatgtttatt agagtcattt catttcacag
      301 acacaccttt tagctgtgat tcacaagctg ctgtagaatt agtaaaagcc tgacctaaat
      361 cctacagcgg ctctccatgg gtggactagt ttcttgtatc actacatttt ttattctgca
      421 acttattaca gtcactactg tggagaaatg cataatttgg gaaaatacga cagaacaatg
      481 tcctgatttc aagagtttat atgaattcac atggacattt acagcccgtg aaaaacactc
      541 gccagatcgt tatttcacag ttaattacaa atctaattag aatattcaaa ttaaagcttt
      601 ccctttcttt tcttctctgt gtgagagcag ggaggcaggg aaagagtgat agtgagaaag
      661 ggagagagag agagaaagag ggacagagag agagagagag agagagaggg agactcagtg
      721 agtggcaatg tgataacctg taatcggcag tgctgcaaac accttcttcc tttgatgtac
      781 ccccactaac atcctggcaa caaagccggc caattacaaa tgagctaatt aactaaattg
      841 ttgatggagc atgaagagtt cattcgctct ggcggctgca ccatgacaac agtgaatggg
      901 ggcctttgtt gtggaggtaa gtgtgcggcc gtgcgagttg ccattagctt ttgacgcttt
      961 ttcacaaacc tatcgggccc ttccacgccg ggaaggagat ttgttatgga tcagcgttgg
     1021 tttgagtcct gcgctgcttg agctgaaccc tgtcaactgg ctgtctgcaa ggtcaaaatg
     1081 tcctctttga aaggcagtga tgttttcttt gttaggcccc tcagcaccca ccgaaagggg
     1141 gaggtgctgt ggtgtaaaag ggtaactttt gtgggtttca caataaaata gataccagct
     1201 tcttgtacgg acatgcaaaa ccccacacgg tgttaacagg gagaggtgag gaggcttcat
     1261 ctccagtgta acatctgtgt catggggtgt gctcttactt caaaaactgt ccaaagtcct
     1321 cgcagatgtt ctcacagccg ggccacttgc acacgccgtg accgtacagg gggtggtttc
     1381 ctccagtttc ttcatgtagc gaactgaaag aaggaagagg agtgagggag gtcaggagag
     1441 tgaaaaaggg agggaagcat cgaatctgca aaaggtctga gtaggtttgt gcacgtgagg
     1501 ttacaatcag cggagaaggg gtcaaaacag gtcagttagg ttgacttctt cataactaaa
     1561 gacagaagag ggagtgaaag acagacacca aaagactgat taaaacgggt atcacaatat
     1621 gcaactact
//
LOCUS       KCVB01000717            1454 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34099pri ultra-conserved element locus
            uce-1015, sequence.
ACCESSION   KCVB01000717
VERSION     KCVB01000717.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34099pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1454
                     /note="ultra-conserved element locus uce-1015"
BASE COUNT          409 a          286 c          331 g          428 t
ORIGIN      
        1 ctatctactt gaaacacttt acatgcttag atgcataact ggcacagcca gagagagacg
       61 gagagacatt tcttaataaa ttacttaaaa tgtcctaata taagtccctt gatttgggta
      121 gcctgggctg caggctcaac aaaatgaggc cttatcttgt aataagttct aacaagttgt
      181 atgatttagg agagccttca aacatttgtg acagttggag aaagaatgtg tacttgactg
      241 ctaagtttgt agtatgtact tgtagtttgt atgcccaccc acaattatgc cccttgatgc
      301 cctgacaact aattttctct ggcactgaca atgtgagcag atatgtggtt tttaaagttg
      361 ttgcactttt tagttaaaaa gaaaacttca ctcaagtgtg gtaattttgt ttgtactaaa
      421 tttgtactaa tggtaacaga gaaggagaac aacattagat acagggaagg ggggcagaac
      481 agaataaagt tgaactcttt acactcatgt tggtcttgtc gctgctggca gaggacacac
      541 tccatctttt ggcagctttg gcgtcaacag gtggagattc tctatccttg tctgcactgt
      601 gaatcttcct gttcaggtcc tgaaacatgt cctggtggcg tttccgggtg tgcataatct
      661 tctacacagg aacattgcac attatgtgtg aggtaaacag aacaggctac catcaatcct
      721 actttacccc ctccacattt aatgtcacga atcttaacac tacagtcaca cctaaacttt
      781 ggataaatct gataaatgta ttcaaacatc tagaggtctg gatagctaac taagagacat
      841 ttacttaagt tttttggtct aagtctaaaa agctctgtag gaatcaaaga gactaaatta
      901 cctcaaagtc aagctctgca tagcgtgatt ttcgtctctg ggggggagat gagacaggga
      961 gattaaagat gtgtcttgat attgtgtgcc ttgtgagcag acaagtgctc atgtttgtta
     1021 actgtttcat tgtgtatctc cacatcagcc agtcaagaaa gggtgtttct gggacccaga
     1081 ggagagcagt gagtcatgct gctggtcatt ctgaccctgt tggcacaagc ttgcagttaa
     1141 cagattgtga ccgaatgacc tttgagtcct ccatcaggga gtggtgaagg aaaacttgac
     1201 cttcaagccg ctcggttgct gtggcagctg ccacctcgca ctgcattatg gaagtgtgtt
     1261 tgccccgaag tgaaaagaca ccagcaggca gaggtccaga ctgcagtgtg ctgatcactg
     1321 tgtcatctca tcctgccact gttgtgtgca ctagaagctg agtaaaaaac caaccttttt
     1381 aaagatgatg cccttacatt gggccagaaa atactgggat acttttttgg atgcagtgct
     1441 tgttatgaat gaaa
//
LOCUS       KCVB01000718            1605 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34145pri ultra-conserved element locus
            uce-1050, sequence.
ACCESSION   KCVB01000718
VERSION     KCVB01000718.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34145pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1605
                     /note="ultra-conserved element locus uce-1050"
BASE COUNT          523 a          294 c          261 g          527 t
ORIGIN      
        1 acaaatgtgt catatatatt ccaattggtg ttatagactt gagttatatt taaaattatt
       61 aaatttaatt ttctttatat tcaggctctt tattagaatt aatcagacat tgctattgta
      121 ataattcaat taagatttat ctctgcaatc taagggtaaa tctttgaatt tttaaataaa
      181 ttggtctcag agaatccaat ttacattgaa atcagagttg ctctaaatat cataaatata
      241 aaaataaaaa tgtaatcctg ttaatgaatg taaatatttt gtaaataagt gatatatttt
      301 ctcccaatat tgcattaact cattgctaaa ttatagacgg atcagtttaa aaatccacat
      361 agaatataaa tcagttaata tataggccta tttaaactgt aacttgtacg tcaaagctct
      421 gttttcagtc ttttacatgt tgaaatatta caataaaaaa tgaatgacat gtgtttcagc
      481 ttgttaaact gcctcaaaag cctcaaactg ttacagcctc tcaatatctg tagcactgtc
      541 ttgactgact ttacgctaaa aaatgtacct ccccaaggtt acatagcaga ttcatttagt
      601 ccccactgtg ccacccccct tctccctctc cctcttttcc gtcctcctcc tcctgctcct
      661 ctttctttcc catgtccatt agaccttgca gactaatcga gcagcaccaa ttgctcccgt
      721 ggtgagatca ttaagtaaat tattttaaga tcaaacggtc agcagccaca atgaagtcct
      781 gtgtaatggt aaaagcttca gaagctgcca cccacttctc cctctctcaa attaaagccc
      841 agtagggaga gctctgcacc tctcaggtgg atgcccccac cattaggcct tttagctgca
      901 ggctaaggta ggacccacta attggtcctt tgaacaaaac taacccactg acaggatctg
      961 acttgtatgc agctaagagt gcaaaattgt ttctggccaa agacagcagt tagcctatgg
     1021 agaaagtcag aattgacttg cttacggtgg aacatatggg tatttgtatt taggggattt
     1081 atagaggggg agagacatct ttattttaat tgcaacaaga agtggtgttg taaaaattgc
     1141 ctgtactggt ttgtgctgaa cacctgaact gaaaatttgc atgatattat gtaagccctt
     1201 gtgtaaaaga aaaaattata ttcaagcaga tgtatttttg gtgagcttac tatttgaaaa
     1261 actacatttg caaaatcttt gattgtttta agtgcaaaaa ttagaaatta caaaataaag
     1321 tgcacaaaaa aattgcatgt acttactgaa agccctgtca gtggcgtcac atttgtattg
     1381 ttgatggctt tgaagagcca agagcatcct gtatgcatgt atgactgtta cattggagaa
     1441 atacttggca aactacataa tcaaccagga aatgtattaa ttttaattct ctacttttca
     1501 aaaagcaaca catttgatgt atgagttagt acatttaaat tttgagcata ttaaaacaaa
     1561 tcactttact ctcctgtata tctaaatagt gttaaagaca ctaat
//
LOCUS       KCVB01000719            1630 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34183pri ultra-conserved element locus
            uce-979, sequence.
ACCESSION   KCVB01000719
VERSION     KCVB01000719.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34183pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1630
                     /note="ultra-conserved element locus uce-979"
BASE COUNT          476 a          354 c          358 g          442 t
ORIGIN      
        1 tgtgaacgca gccagctttg gaaaggtaag actcaaacct cagtggagac gaaggttaaa
       61 ctgtctgaca acacatcatt catcacctgc atccagttta actcaatgtc atctttaaaa
      121 gtattgaaaa cacatgaaac atttgtggtc agtgtctctg ttcaaaatgt ttgattaaca
      181 atgtagagga tgcacagctg agcaagtctt ccacaaggtg acactgaatg agctccactt
      241 ttcagggttt taatatagac ttttgcatgc actgtcagtt tatagtaagc agagcaaaat
      301 gactcaaccg aagaggatac aaaacaacat tcgggtgctg cagctcctat gaaagcaatg
      361 ttctcacaca ctaaatcagc cataagcatt agtgtgacca tttcatgcct gggttggttt
      421 tgtgctgcag ctctggctgc tgctgaagca atatcaactg cagctcagtg tgaagcgagc
      481 aaagctctgc ggtcgctcgc tgaacacata atctagagcc aaaggagatg tgctttcgtc
      541 tgttcccaca accccaagaa gttcctcagc cttcctaccc ccagccatcc cctgtactct
      601 ggactctggc cccaaccccc aacccagccc acccccgccc ctccgcagct cctgtcggaa
      661 gatattgaaa aagagaaggg gcctggatta atgtgtaatt aaaggaaatt tggaagggtg
      721 agatgttgcg gaatgttaga tcagtggctg gagcagtgtg gggtgtcctt taggaaggct
      781 tcattgattc aggacaaaaa aacgagagag ggcccggact gcctccctgt ctgctcattt
      841 atcaagcggg gcctgacaga aacttcacag cttggcctct tttttcatcc cacacaatgg
      901 agagctacac tgtttgctaa agtttctgca ccaggagctg gaccaaggag ccaatctctt
      961 tggacaaaaa gagaggaaag gaagaaggag ggagaggaga agagaaagag agtgagaagg
     1021 agagaggatg ggtgggagga tagatgaggg gagactgtgg atataggaaa aaagtaatac
     1081 ccctggatgt cataaataga tggctctcaa agctttcttc cccctcacag acctccctgt
     1141 ggacacagca gtgagacttt catgttgttc tcctgacaaa ctttattaaa cacttttctc
     1201 ataaagagct gaagttactg tacaagtgca gtattttctg tctaataagg tcatctgtat
     1261 gtgctgtgta ttagaagtaa aaactacctt tttctgttac acagtatttt cttctattat
     1321 gtgaaaaata cacactagtt tatttttgat agagatctta tttggatcct tgtgtcacct
     1381 ctttccacct gcttttcttt ttctctttac tttaatgaat tagtgtgaaa ggctgagatg
     1441 aaattgatgt aaaaaaatta aatctctgcc tctgaccact ttttccctcc tacaatgaac
     1501 agatcataag gcagcagttt ccagcgttaa ccaccagaag acttgggacg agaggccagt
     1561 ctaagtaagt tagttggctt ttattttgtc ataaactgcc aacttacaca cttttccaaa
     1621 cattcacaaa
//
LOCUS       KCVB01000720            1226 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34219pri ultra-conserved element locus
            uce-1108, sequence.
ACCESSION   KCVB01000720
VERSION     KCVB01000720.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34219pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1226
                     /note="ultra-conserved element locus uce-1108"
BASE COUNT          422 a          221 c          290 g          293 t
ORIGIN      
        1 caaatgattg aagacattta aaaagactct gattaaacct ttattgcagt gtgaaattaa
       61 gaattccttt gtaatcatca aataacggct cacattttta gtttctccag gcccagggaa
      121 tgaacaaagc tttattcatc ccaccaacta tgcatcactt aaaatataca tcacatggat
      181 catataatat ttatgactgc ccttacttaa ccaattcaaa ggagtggctc tcattctgca
      241 aaggacatac tgttggacat gactggaata gtaagaaagc acagatatga aaagcccact
      301 ttcattccaa tcatctcttt ggtttgtccc tttgcttcaa gtccacacca tctcttctta
      361 gtccctaatg accctgttgt cctagtaagt tccaccaaaa acagcccaaa ccccccaacc
      421 accccccgca cacattaaca cagtccccca gcctttagga aggctcgact tactctgata
      481 ggatgtacgg atccttttct ttgaatctga ataaaagtta gacaggccac gcatgcaaaa
      541 ctctgtcagg caggcccaca tgcccacacc agcagccaca gagtcaccaa aaggttgggg
      601 ggaggagaag gagagggaag gaaggagtgg tggagtggag ggagggcagg ggtaaagaga
      661 gagaagtgtc agtagatatt tagggttgat agagatggga gaagacagga gggtgagaaa
      721 gagaaagagg tggcggagag gtgtaggagt aggaaaaagg tagataagga gggaaagaat
      781 gtgaaggtaa acaaaagagg aaggatgcat ggggaaggaa ttgagtagag acattttggg
      841 atggagggaa gggaagggag gaaaaaggag ggaaggggga gagaaaagaa cacacaggct
      901 tagagtgaaa aagaaagtga gagcaagcag caaccgatgt tagtgtcagg ggaggacaaa
      961 aaaagatata ccgctgatgt cattggtcat ttgcagaaga agtaaactac tgtgtaagca
     1021 ggcaacatat catcactaac aaagcagcat cagaataaat gtcttgtctc attaaataat
     1081 tggttttaca tttcattttg tttacagact ttttgccttc tgtcctaaca tgttcaggct
     1141 ggtattaata atgcacacat tcctgaaatg atgaatattt aagcatcatt taataaaaat
     1201 aatacactaa ccttgatttt acatat
//
LOCUS       KCVB01000721             897 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34257pri ultra-conserved element locus
            uce-617, sequence.
ACCESSION   KCVB01000721
VERSION     KCVB01000721.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 897)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34257pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>897
                     /note="ultra-conserved element locus uce-617"
BASE COUNT          256 a          168 c          195 g          278 t
ORIGIN      
        1 aaaaaaaaaa aaaaaaaaaa caaatcacca aatgtaacaa tcttccccct gtcctccttg
       61 tgattgcatg ttaggctctg aggtgataag cacactggta cgtgttgttg ctgtgtgaaa
      121 gacacccttt atcggctcat acctctgact tgtgcttagt ctagtcagcc ggagaaatat
      181 gacggaaatc tgatagggga atggtcccat ctcaacatca gcgcaagtgc acgccttgca
      241 agccaatgtt tatgcttccc gggccttctg ttctcaaggg aaaaatgtca atagagtgtc
      301 acagggagca tcttgccaat gagaggctgt cgtctgacct aaacggcaaa tacatcagag
      361 ggtgcagaat catgaaatcc tcactcgggc gttgttattt tgaagattaa aatttagaaa
      421 acagagagtt agatatttga actggtctgt gtaaaaccat gcatgcaaag ggcagccatg
      481 ctgtgatcat ttgtagctag ttaacatgcg tgtcagtaca tgtggatgta aaggattttt
      541 ctttctctcc agtcgtgttc agcagggcgg tgagctcagg acggagtgtc tctaattacc
      601 gctgtgagag aggtcatcag cgagaatgtc acacacccgt cgacttcacc gctgttacat
      661 tatgaattat aataatataa tgttttgtta gaagacaaga agtttgattt tagtcttttt
      721 ttcagatcga atcttgtttt tgagggtttg gacattttta tatgatatga tatttgtgtg
      781 cccgattctc acatgagact gaacactgaa ttgattttta atcaaatttt aaaagatcgc
      841 catcggatat ttttatgact tattgtcttt ttcttctctg ataacttctc aaagggt
//
LOCUS       KCVB01000722            1000 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34297pri ultra-conserved element locus
            uce-283, sequence.
ACCESSION   KCVB01000722
VERSION     KCVB01000722.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34297pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1000
                     /note="ultra-conserved element locus uce-283"
BASE COUNT          304 a          189 c          210 g          297 t
ORIGIN      
        1 aaatctgctt cttttttttt ttttattcat gtctttccag aagattacaa gatttgcaga
       61 gatttgatct gtccctctac cactgctgta ggtggccttg acattaaagt caaaatgaga
      121 actttaaata agcattacca cccatatcag cttttttttt tctttttttt tcacagcaag
      181 tgctttggaa gagatgtaat gcttataaag tccgacacga gagagaaaaa aacaggaata
      241 caagggtctc tcagttaatg tatcatcatc tgagtgggtt tggattggag ttattagagt
      301 atttcacttc attgcatgac ctcctgttcc tcctcagatc atgaatccta ttcattaccc
      361 agtccttctg gagtgcccgc ctttataata ttactcaatt tatattctga agcgggctcc
      421 tgggagagcg atcagcgctc cgaaacagcg acccccctat aacccaatca caatatttaa
      481 agggcagcaa agagcttttt aatgcgcgtc gtagataacc atttaaagtg gattgctttg
      541 gtttaatatg accttaacag gctggcagtc acatgtgaag aatgaataag ctaataggag
      601 agacgacagg gagaaagaat gaaatcaaaa agcagaatat gagagatttg tttcaatgca
      661 agctggcctc ttgacggctg ctgagaggag catgatagag aatcacaagc gtgtgaatga
      721 aacaggacag gcgatgaatg aagctactgc ttgcatttct cttttccctt ttcccagagt
      781 atgaactgct ttatcatgat aaaagagaag acaggtataa atgacacatt tacattctcc
      841 catcaaagag aaacttgact caatctcgat gaaagcagct gtggatctgg gttttacact
      901 gtgttctctg cagggggaaa ggcagttaca ggatgatata gtggcaacat gcgatgtttg
      961 tttagataca tcatgaaaaa tatctttgct gggaaccaac
//
LOCUS       KCVB01000723            1232 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34341pri ultra-conserved element locus
            uce-1209, sequence.
ACCESSION   KCVB01000723
VERSION     KCVB01000723.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34341pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1232
                     /note="ultra-conserved element locus uce-1209"
BASE COUNT          363 a          252 c          323 g          294 t
ORIGIN      
        1 gcaatatttg agcattgtga gcggatgaca tttccaggag gatgtcttga tgccttgcag
       61 agcacggcaa tgatgggaaa ggtaattcac atacagtatc tacaggagag ggaaaggcct
      121 tttgcctaga gagacagccg actgttattc gaccctcaga attgaggtga caccatgcta
      181 tgctaatagc tacatcccct gttgatatca ttccacacgt tgcaagagac gatttatgct
      241 cagtaaggga gaaaatgttt cctcaatcgc acaggtgttt gacaccatgc ctgagctcac
      301 aatgttatct ccagctcaac cagagaaaac agatgtccag tgtctcccta tagtgcagct
      361 tcccactccc agcgggcatg cacagagtca ctcagtcacc cacgcattaa agagaggcag
      421 ctgccagagc attagtgtcc aaaaattcag tgggagacag acataaacaa gcatgacagt
      481 tggttttggg gatgcgagga acgctgattg acagtgctct gtcggccgga ttagagacgt
      541 tcatttcgtc ctgaactcaa ttatatttta caaaggctga catccccagt taattgcagc
      601 ctttccacat gagggaatta tttgcacttc tcaattccct ggccatgtgt actctgttcc
      661 gcatgtacag gcttcatcta atatgtggaa atattgcaaa ttagatcagt aaaaaaagaa
      721 aaaaggaaaa aaaaactggg aaaagacaaa aaacttaggg aagtgtcaag aggcagagag
      781 aagtgaaata ctggtacagc ttcaagagag gagcgtctgg gtgagtagtc tgcctccgtc
      841 tctcaagtgt gtctggatct tagctcagag acagcttagt tcagagacag atccagagtg
      901 acagcattgc gatgattgac acgctgatga agaggagaag aggagaggaa gagaggagaa
      961 aaaggttgtg cagcgccact ggctccactg taacgaaaat gtgagtcttg cccatggttc
     1021 gatgtgacag gaatcccagg ctgggcttgt gccgaggagg taatgtcagg gaggtaatgt
     1081 caaggaagcc cacaggcccg tgtacacgaa tgtctgtgta tgtgtgcaat atgtgtctac
     1141 acacatgaat gtgtttctgt atgactgggg gtcggtagga gaataaagag aaagactttg
     1201 gggctgtata gcctggatta ccccgccatt tc
//
LOCUS       KCVB01000724            1431 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34384pri ultra-conserved element locus
            uce-516, sequence.
ACCESSION   KCVB01000724
VERSION     KCVB01000724.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34384pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1431
                     /note="ultra-conserved element locus uce-516"
BASE COUNT          496 a          240 c          284 g          411 t
ORIGIN      
        1 gagttcaaag tttgcaagat gcccaagaaa tgagaacttt tcttgccatc ttctaaaccc
       61 tgaccccagg tttatccagg tatctcacaa acaggtactt aaaagtacaa gagtacacct
      121 gagaggagaa tatagggact gaaagagcca aggaattctg ctttactttg cctgtgcttt
      181 tgctcaggta gcaaaatcac aggaaactac tcagatattc ctttttctct caatccccat
      241 tctactaaaa tatataaaat aaaacataca aacgttgaac ataaaagcac tttcagatgg
      301 cacaattgtg tgcaaattat tgtgcagcct tatggaatca catgtagcta taatgcagta
      361 cttgtaaaaa ttgaaaaggt aatacaagga tgccagttct ttgctagagt atttttttat
      421 atgagagctt taaatgaact tagataatca gtacatataa tagatattaa ccagatcctt
      481 gacaaaatag ttggcatcct tgaatatttt ttgcttccaa tggtaatagt tttcttcatt
      541 attataaaat gaaaaccaaa tatttaaaac acaaataaat gaataaacaa ataaaaaacg
      601 catctcataa ggctacaata agaaagtaaa atgatgttcc gaacctggaa ccacttagcg
      661 tggcgcagag cagccagagc gtcaaggcat ttttgcctgt ccaagacgtc cgctgggtct
      721 ttcaccatac ccgaagttac atctaaaaat ggatttgaag tgtcaaaatg gtgataagga
      781 atgggtgttt gaccaggtca gcgaggctgg caaagaacaa acacattgcc acacatacca
      841 atgttttttt tccatgacaa cctatagttg gttgcttaat ttgtaatttt aaaaaaaggt
      901 aaaggatatt aaaaaaaatg actacacaaa ccaacacact gccaacatta agtggatgga
      961 tacagattga ctgtgtacag tatgttaagt cattatgttt cgttagaagg ggtatgaatt
     1021 tactgacccg acattaaaga taagtagtct gatgtgtttg gtaggcaata atcaagctaa
     1081 attacaaggg attaggtcac aaatgtctta aggccaatac agttttatca ggtaaatgtg
     1141 ttgtaaagct taccattact ttggtaagta catgggtatt ttgagtttta gcagattttc
     1201 ttcgcacaag cattgggaca gaggagggtt cccatggcta tgattccaag tattgcactt
     1261 ggtgtagaga gaaagaaagt gcaaatggct aagagcgagt gacctgataa aaaagtgaca
     1321 tggcaatagt aggtgtggca aagtttttgg gatgtgagaa aaccttggtc aaaaatcctt
     1381 ccgaagctgt tggtccctaa taaacctctt atgtgagaca atgtactgga t
//
LOCUS       KCVB01000725            1483 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34428pri ultra-conserved element locus
            uce-553, sequence.
ACCESSION   KCVB01000725
VERSION     KCVB01000725.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34428pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1483
                     /note="ultra-conserved element locus uce-553"
BASE COUNT          400 a          290 c          290 g          503 t
ORIGIN      
        1 ttcacaactc aacaaaatat ataacaaaga tgtagactgt tttggacatt tcagtgtgga
       61 aatgctacat attgtatatt taaccaaagt gccattgttg cctaaataga gactgaagtc
      121 aaaccctgaa ctctgtgtca cttttaacgt ccactgtcca ccctgctatg actccagcat
      181 ggcacataaa tgtagtattt aatttactca aataaatgtg gtgcaggctt gctgagtgtc
      241 acaaaaaaaa aaagaaaatt tgtgtccatt cacacaaatg ctatacatta aatttagtga
      301 ctaatttacg actatttcca actatgttgg aatgaaacat tcatggatat cgaaaaaata
      361 actaatggat cactacattt attctttata ccactggcaa agtttgtcaa gaaaataaac
      421 tggtttccac gtgtttgttt gtgacagcct agatgtgagc ggtggagaca tataaatcat
      481 gtcacattta tttttcctct caacttggaa catggtcttc ttccctgtag aattcagaac
      541 tgatcattaa cctttcctgt agttccttcc cttgcctggt cacatttcct ctctatttgg
      601 ttcctggccc catatgccac tttcctattt cctgcctgca gacagcaggg agtagctcta
      661 ttatcacctt tctagagctg tctttgaatg tgagaggcaa ttccatttca ctcagccatg
      721 gggaggagga ggaggacgag gaggaggatg aggaaaaaag cacactgtag agaacacaga
      781 aattggttta gggttacttc ttatagtcga atgtaagatt attttgcttt ttcttggcca
      841 gatttaagga ttatatgatg ttctgttgtt gaggtgcagg tttttgcagc gtagctccaa
      901 atcagagcag aaggttaagt gccgtgtatc atgtctgttt gttgtgtgaa cactttatcg
      961 tcaactccgt tttagaaggc atcttttgaa atattaacaa tcttaaagca gctatattac
     1021 actgctgtga agctgtgcta aacatatcga agatgtctct aatgcttcca ctctcaagtg
     1081 tgtgctctcc attttgcgac attttactcg aagcctgctt tatttttggg cttccctgag
     1141 tcttcttcca gcttttatca gcgtcgagtt ctttggactg ctagagctct tgacaatatt
     1201 gctgcacgat aaattaaaca tgagttacgt agagtgataa tctgtgggcc tctcttttca
     1261 cactgcattc ccaatgagct gtgtgttggc ctggtgttgt tgcctgtgat tttaatgaag
     1321 attattcttg taacacgaca gtcgcctgtt tgctcctatt ctcccccaaa tattttctct
     1381 ttaagcggag aatttcgttt tctttcaagg gatctgtgca gcatttgtgc tgtattttaa
     1441 aagtaccttt gtagcaggtc aatcattagt ttatcacgga tat
//
LOCUS       KCVB01000726            1466 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34465pri ultra-conserved element locus
            uce-206, sequence.
ACCESSION   KCVB01000726
VERSION     KCVB01000726.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34465pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1466
                     /note="ultra-conserved element locus uce-206"
BASE COUNT          485 a          256 c          274 g          451 t
ORIGIN      
        1 gttactagaa tttgaaatgg cacatttcta gtgcagggtt tattacacag gaaaatacta
       61 aaatatattt atgacatttg aacacatatt ttttttgtat ttgtttattt tggtaatgag
      121 gaaattaagg ctttgtttgc ttctacacta caatcacctg ccataaacaa ataaaagctc
      181 ctaacattgt ggaattacac ttaacaccag atgtcatttc aattgcatga tatttgagaa
      241 atactctctt tatatatggt gtatatgcaa agagtgtttc cttactttca gtaaaataag
      301 aaaagaagtt gtgctatcat aatatgacag ggaattactt cactaaaatt ccaaaagtac
      361 aaagagcttt ttgttggacg atattccttt tccaatatat ttcagttatt aggaggagtc
      421 ctttgtattg agtagagagc tccaaaaaca ctaacactgg aggaataaca tctgcatgtt
      481 tactcaggag aagattcatc cctgatagcc tccacgaaaa tgaagagtgg gagaaaaaac
      541 aagtggagga aatttgtgga atcccatctg agattgcttc cccgatggat tccttttcct
      601 gtcagctcag acgagaaatg aagccgggga aaattaactc ctgatacagt tacgctgaat
      661 tagacagaga gggggaaaaa aacctacaca ctttttaagg attccagcct tgtcattttc
      721 aattttctgc tctttggaca tgcttgtatt cagattcccc aacatctatt cgccatatca
      781 atcctaatta gacaatctgg gcccacgaag gatgggagcg gagttatttg cataatttaa
      841 tcataaatac tcagtgatac atatttccaa atgcatttgt acaattatct tttcatcctt
      901 ggggcaaaag tattaatatg attaggcaat aattctttaa aaaaggggga aaataggaaa
      961 ccagcaagtg tgcactcttt tttaaactcc agctttgggc tatatgaaaa attaattaat
     1021 ttctgaggaa aatcaatttc tctatgtgac catattagac agagccaggc aagggcctgc
     1081 attccttctc caatcaagaa atctcatctg ctcactctaa cttctgtcag tagcatttaa
     1141 catcctggta cgagtgtcag tgacttccgc catgctccct aaatgtgctg taatgtgaag
     1201 gagccgtgag taaagacaag caagggagct ctgactgggg agagaggaga cagaaatcag
     1261 agtgacccaa aatctattgc tggcctgtca ggcaatcagt ctcaagtgta aaatcatcag
     1321 gtaaaatcac tgttaacaaa tctagtttaa tatgctaggt aagtcatatt gacagataga
     1381 ttttaaacag cttaagtctg ttgtctatta aaattaaaga taatacctct ttatgattgg
     1441 tgaacatagt aaaaataggt catttt
//
LOCUS       KCVB01000727            1290 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34501pri ultra-conserved element locus
            uce-243, sequence.
ACCESSION   KCVB01000727
VERSION     KCVB01000727.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34501pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1290
                     /note="ultra-conserved element locus uce-243"
BASE COUNT          323 a          315 c          342 g          310 t
ORIGIN      
        1 cactagctca gtgtaatact gtgagacaat atgacaggcc tgctgtgcaa ttctcagaag
       61 ctgtcatggt gatttgaaaa tttgaccatc acatgccaaa cactctttgt ctggaagaat
      121 tttgaagagt aagccacatt tcctggacca tctgcctcat ataaattaca caagacaaga
      181 ctggcacccg caaacatcca caatgttgcc tgggagacag aggtcacatg agaggatgag
      241 tcacagaaac ccagaactac ctagaaacca acactattga acggcctcac atcatggtgc
      301 tctttacagt ttggcgacat gcagaaggat tagtgtcgtg tcagtgccta aacgacagtg
      361 agacggtgcc cttggcaaaa gacaagtata cagttaaact taatggggcc agaatctgat
      421 acctcaatag gaatcaactc cagtgccagt tactattctt tactagctgc acacacacaa
      481 acacagcagt atgaagatgt atatcaacct gtgtctgacc atgggtccac tgctggtgtg
      541 tgggttagtg tgtgtgttcg tacggacctg gagagtaggg agttaatgca ggcagggctg
      601 tggcagtcct cctgcccctc agccagatta atcatgcagg agagacacag caggcccccg
      661 ttcattctgc ctgggggtga agctgtgccc gcatgctctg cgtatcacat ggccctggcc
      721 cccctctctt ctctcctctc tcgctctttc tctctctact gggggtgctg tggatggcgc
      781 tggtggtgga ggaggagtgg gtgtgtggag ggggttattg gcggtgtggt ggaacgccac
      841 agcacgcagt atagctttct ttctttcctc cacactcttt cctcaccccc ccccttctcc
      901 cttcacttcc ccttctgtgg gtgtgaagcg agtgaggcag aaaaaaaaaa gaagaagaga
      961 gaaacaggcg tccatctctc ctgcaaggag acaacaacag cctctttttt tatctcccgt
     1021 cggagggccc tgacgacagc agtggccagg ctcggtggat gaagaggaga agtgtgtggg
     1081 gaggaggggg tggaggggca taccaaggga ctcgccacag atatctcctg gaaactgctc
     1141 gacgatgtct ctgcgcttca gagaacagcc acagccattg ttgggctcgc tgggaaacag
     1201 agaaggcatc gcacagttgc gtgcttcagc aacaagcacc tatagtgtgt gtacagtaga
     1261 aacttaaatg tttcatgatg tgttttgttg
//
LOCUS       KCVB01000728            1542 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34542pri ultra-conserved element locus
            uce-692, sequence.
ACCESSION   KCVB01000728
VERSION     KCVB01000728.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34542pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1542
                     /note="ultra-conserved element locus uce-692"
BASE COUNT          386 a          323 c          369 g          464 t
ORIGIN      
        1 ctttctcact ttctgaaata cggcctggtt gatccttaaa gtattttgtc tttactcagg
       61 ctcgggtttc tctgtgaggt tatttgatgg gtgatgactc caaattaaag tgctgacttc
      121 tgctgctgtt gatcgggtga attgaccttc atagtgattt tactcagtct gagatactaa
      181 gtggaacaga aattatgagt aacgacagat tttgtgtgtt tcaccaaatt ctggacagaa
      241 gcttgaaatt tcttaatcaa accacatgga agtataacga agatgatgct ttttcttatt
      301 aataacaaga tgaggagaaa cgggggttct ggtttggcag atgacaaacg gtggtgtttc
      361 tttttcctgt gcttgggagc cagcatatgt taccatgtta atgaggtgta atggtgggaa
      421 tccagcatgc tgacatatgg ttctcagatg actcctttaa tgatattcat ttgtgtcgtc
      481 gttattcagt gagttgttta cctcatgtta ggtcaacatg tcactggctc gtgattatgt
      541 cacatcatct gtctcaatta tcgaaaatga aacaaaacgc agcttctcac agtaggactg
      601 aagactctaa tcagagagcc agctgatcga aataaggaat acgagcagag gcaaagacat
      661 gacgtcacct cagtgtggat aatactgtat ctttattcat gttattccct ttcctccaga
      721 ggacttttgc cgttaaaaag ttaggcaaca caaaaataga ctcgtggatt gtgatgccca
      781 ttatgacaat ataatgtgtt gttgatcctg tctgcacata taggggaaac agcgcactta
      841 attagggcct tgacaagcct actctcctaa agtgatgaga ttaatcataa ttggtgtcac
      901 tgtgaattag agaccgcttg gatgaccgcc ccactggcag gtcggaccgc agtggagacc
      961 cttggacact gctgctagcc gcagcttagc cctctctggc tccctctttc cctctctgtc
     1021 tgtctccctc tgcccagcta tctcaggccc ctctcttgct ttgccctccc tgggtaagag
     1081 gggcgcagat ctcagccaac agcctaatga ccccgtttga agttcttcta aatccccctc
     1141 gaccctgccc gttacccatg acccctctcc taagacggtt cgaggttaaa gagggacagg
     1201 caggttgacg ttaagatagg ctgaagaggg agagaaggct taggcgagtt gttgggggta
     1261 ggagggtggg gttaaggtgg attctcacta cgttataggc caactcacgt taaatggtaa
     1321 agttaagtag tgctgcaatt tcatttgatt cctttgttgt acatcttaat cattttttga
     1381 cctcttttcg tgtgtgtggt gctgtgttta ggttccaaaa ggccatgtat ggctagaagg
     1441 ggataacctt aggaattcca gtgactcaag gagctatggc ccaattccct atgccctcat
     1501 ccgagggcgt gtttgcttaa aggtaacaag ggactgtatg gt
//
LOCUS       KCVB01000729            1407 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34599pri ultra-conserved element locus
            uce-1234, sequence.
ACCESSION   KCVB01000729
VERSION     KCVB01000729.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34599pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1407
                     /note="ultra-conserved element locus uce-1234"
BASE COUNT          400 a          291 c          321 g          395 t
ORIGIN      
        1 agatacactt ctgcacagaa tgaggactgt agattttatc tctcatcaca tacggtaaaa
       61 ttgcattagg aagggatttg aactctttca acatacatat aggtgtgtga ttattgcttt
      121 aaggtaaacc tgaaaaaggt gaaactgtcc tttaactgat tagtgatcag caaaataaga
      181 ggtaacaatt tagtcatatt tcaagcttta aaatggtttt tccaggcctc tctaaaattc
      241 tctgcttttc tttgtcttat atgattgtaa atcaaaacat ccgtggtttt tttttaaatg
      301 atgctcagca ttcttcattg ttttctgact ttttatagac caaactctta atcaatgaat
      361 cggcagagaa taaatcgata atagaagcaa tctttatgca tcccagcgta cagcatctta
      421 attgtacatg ccgcgcataa agaagtagca acaaatgctg agaaaccaga aggagtgcaa
      481 gtaacatact gtactgcaca tttctgagtg ggaacgcaac catagccagg ggaggaaggg
      541 aaaagggtca atggaccgat acttgccgat actcactccg agatgacagt gaaaggaaaa
      601 ggtaagagag actgggatag aagtgggtgc taattcctac ttttcagttt aaataaagat
      661 tggagctgga taaaagccat taagggagaa atcagacagg gcatgctatg taggacaaga
      721 gctgactgca gggaggtgag tggtgggatc gatactcagt cgatgcgctc aacacccagc
      781 tctgcacaca cacacacata cacacacaca cacacacaca gaggggcatg tggtattgcc
      841 gggcatctgc atgtttgtgt gtacatgtgt gtgtgtgcgt agcagtgact aatggagaaa
      901 gagagggagc ccggagagaa agagtgcatg caatatacaa ttaactctct tttgagtgag
      961 ggaatattaa acacaggctg cttgtgcgga ctgtaatttg tagtgataaa agtattaaag
     1021 taaagcactt ggacaaagat ggaagggtgg acagctgcag agatggattt attttcggag
     1081 gcacgataca gttaacaaag atagatgttt caaaagagcg tcttcagccc gaactggagc
     1141 ctcaatacga ctgcaatcac tctgcattct gtcctcccct cctctccttc tcctcttcct
     1201 cttctccttc ttcttcttct ctccttcttt attttacctt ctccctccct ctctctctcc
     1261 ccccttgcct cccttcctct ctccctctgt agaattgatc gactatgtct cctcaatcca
     1321 tctctccctc tgtcgcttag caacgggtgc tctatcgggt gtggagggtg ggagtttcag
     1381 taggggaggg gtggggtagg tgggtgg
//
LOCUS       KCVB01000730            1474 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34640pri ultra-conserved element locus
            uce-107, sequence.
ACCESSION   KCVB01000730
VERSION     KCVB01000730.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34640pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1474
                     /note="ultra-conserved element locus uce-107"
BASE COUNT          385 a          317 c          326 g          446 t
ORIGIN      
        1 tagtcggacg tatgtgctcc atgtaattag cacagccctg ttgtggaatc actgtagtaa
       61 tacactctat agattgaccc gtgggcttcg ccactgcctg ggaattagat gtaaacagag
      121 ttttgccttc agattagaaa gacacgtcct cttgtctctt tgcacactgg agatatttaa
      181 aaatgtgagt gtatgggagt tggagaataa taagtatcca gataacgcgg gatttactaa
      241 aagttttatt ctctattata tgttgttgtt caaaattggc attgctgttg atatgcagcc
      301 cattacactc aacttgttat tcacttttgc ctgacatgtt ctaatctaaa tatctcttgg
      361 ctaatgcagt gagatgagtc acaagccata tactgtatgg gtctgagagg cttgttgttg
      421 atcttcttgc attgtaggaa cagtttgcgc cactcaaaag tcagcaaatg ctgagaattg
      481 tcagttttag ttgctctgtg ctttgtagaa aacatacagt ctgagcggca gtgaattgag
      541 atgtgacgtc tttctgcttc taaatgttcc atcaacaccc tcgctttccc taagccctgg
      601 cagagccact gccacgaatt aactaggcta tccaatttcc cttcatgaaa taagagcttt
      661 ttaatgtatt ttttagagaa gcagagagaa gttagcacac aatgatgaat tagatttcaa
      721 tttatttgac ttcattccca gctcgctgca ttagagcctg caaacacaca tctccagcag
      781 tccgtccgct tgattcttat taggtttcag aaaacaaggc gagggaattg cattagtgta
      841 tcttaagtta aaagctcttt gctctttgac caagtgtttc attgttattg ggttaatcag
      901 atttttgctg tcttcatgga gatgctatcg caggctagag gcagtggtgg gtaaccaaag
      961 cggccgtgca cagacactat ccagatggga tcagcttacg gtaatttggt ttcagaatac
     1021 attaccagaa gaggctcctg gccgagaatg gccaccccgc tatgagccag gggctggtgg
     1081 cctctgtagc tgcagcgtcc agcctagctg accagccaac ccacctgtcc cactccaacc
     1141 ctccacatct cccagagctt ttaggagatg ttggatgcaa agagaaaaag taaagaaaac
     1201 ctaatgtatt aaaaattcag tttgtcaccc aattctaaat acttcttcct tgtaccaaca
     1261 gggctttgtt gtccaattca ttagagagat tcctgagtcc actggccacg ctggagccct
     1321 ctcatttctg gtatctagta tcattacttt cactgtaaat atgtcactca gctcaggctg
     1381 agcggggggg agggtggagc atgttgttcc tctccaggcc aaatggggat gcagagtgta
     1441 ttgacactct ctggatgttc actgttctgt agct
//
LOCUS       KCVB01000731            1333 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34686pri ultra-conserved element locus
            uce-944, sequence.
ACCESSION   KCVB01000731
VERSION     KCVB01000731.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34686pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1333
                     /note="ultra-conserved element locus uce-944"
BASE COUNT          397 a          319 c          277 g          340 t
ORIGIN      
        1 gtaaaaaaaa aaaaaaagac tgaacataat taggcagcag ctggggattg aggatgctta
       61 aatctgaaga aatctttgaa gaaatttgtg taaacttagt gtaatgggca aatgggaaag
      121 ccagtttctt gtggaacatc caatgaacct aaaaaaccct gcgtgaaaca tcagtattaa
      181 gtttggtcta tcacccaaag ctgcattagg tctctcctca gtttactcct taattccatc
      241 tgagtaatcc cacattattg gcctctaaca gtaccctaaa cggagaataa aaagtttgct
      301 cccctctagc agtgtattac caggaaacaa caggccctct cctcattaag ttatagatat
      361 aaatgctcca aagcatgaaa ttacccccga gcctccctct ccaaataacc tggtttgcaa
      421 acaaatgatc cgagcacaag cagtaaaagg cagattatta aaataatagg agggatttaa
      481 atttgcatcc acagaacaat tcagcaaagg actgtgcaat tactcctccc cctcctcttc
      541 ccctgttcat tccacctttc tactggcaag tcgagtccct cgcactttat tgtgtccatc
      601 tgttcttatt aaacagaggc agagtttggt atcgtcaggt gtggttgtac gtgtgtgtat
      661 ttggtcaacc acaggagcgt gtgcacttat ccggtggtgt ctggctgttg tttacctcaa
      721 acaaaaccac aatacatgta catgctcata ctttcttatt ctcctctact cttatttctc
      781 tattcctacc ttacactatg aaaaatatct aactttatct ggagacaata acagtccctc
      841 ctcctgtgct ggagtaaatg gggagcgatg agagagtgag gtgaacagga acggacaagg
      901 caaagggtgc tcaggtctgc attacctctg agttatggcc tccatttgca tctcaatcag
      961 cctctttgga gagcccacca ccttggtaac acccccatca caccacagcc accccgggta
     1021 ataagggaga tttagtggga ctggcagcct accgtgggca ccccctggct ggggctcgca
     1081 cttcattccc agcccctggt ctcgctaagg gccctctggg ccacatccaa acacaggaga
     1141 atggcagcac ttaacaagac taaggaagac caaaatagac gagcagcagg aacaggccat
     1201 aaaacagagg gaaaacaaag cacccgagtg attgtaatag atgttccagc gtcgatgtct
     1261 gcatagtgtt ctgtattgct gcctcattat agtgggagaa aaaagaagaa caacttgaac
     1321 ttgtgacctt agg
//
LOCUS       KCVB01000732            1407 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34730pri ultra-conserved element locus
            uce-593, sequence.
ACCESSION   KCVB01000732
VERSION     KCVB01000732.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34730pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1407
                     /note="ultra-conserved element locus uce-593"
BASE COUNT          482 a          260 c          261 g          404 t
ORIGIN      
        1 gaagcgaata agtttatgtc ccaaaaggtt aaactattcc tttaatagct tattaacatt
       61 aataactgca gacttaatgg cttattagaa agtgagaaac gtatttatta ataacttgac
      121 tatgaacgac tcaccagcaa gattgattct tcaaaacctg gcaatgcaaa gttgttctta
      181 ataatgataa taattgatct aaaaagagtt agtagcctca cttctttttt taagctttaa
      241 ttaatccatt aatgatatac atttataaag tgtgccttat gagaaagttg tacggagata
      301 ttttattaaa aagttcctcc gtttatacct cagatgtttt catataaaaa aaagcatctt
      361 acaaatacga gtggctgtga tttagtaaag caaagatcat gtcagtgtat aaaaccagga
      421 cagaaggcaa cagtgcaaat aaaaatgcta ctatctgctg tcatttccct ctagacatac
      481 aaccagtgga gcatttaagt cgctagacgc tcagccaaga ctcagacaag tgaatttatg
      541 tgttaagttg gtgtccccct ccctctccct cactgtctca aaggcccagc acagatagcg
      601 agttccatta ggaatttcaa ctcgaattaa ggtgcttggg gaggacacat taacacatgt
      661 gccgcttctg tagttaaatg tcagaatggg aatgaggcct gtgactttat attccattag
      721 ccactgcctc atgtttccct gctgtgtatc taaataaata atcaagacag cctccaaaac
      781 acgaatcatc ttttcacaat aatagaaaat taatggctct gcttgggtaa gtaaattaca
      841 ccaagaacat cccacaacct aaatcactgc tgagagacga tgacaaagga tgcattatag
      901 gccgcaggaa aaaaggaacc cactcgtcca gaaaattttg aaatctgaat aaaatgttgg
      961 ggctttcaag ctctttacaa tcaccttgca tgaaagtcta attcagtttg gcttcttatt
     1021 aattcataag ccattagtaa catatttctc taacaaaaag cccctgacaa aggaaaaaaa
     1081 gagtgtgttt ggaagaggta cgtgcaatta acatttaagc atgggcgatt aaaaagaagt
     1141 ctcaaagaaa gggcttagag tggggtcctg aaagattcaa agggcggaac tattttatgg
     1201 ggcattagcc agatatattt aaatacagct gaagtgtatt tctgccatat agtcagtata
     1261 acacctgtca tgctaaatca tgtacacaat cggctactat taagatgaat aaagccaagc
     1321 gagagcatta gtgagtgcac tctgcagtgt ttgtaacagt gagcttgaat atagtcccac
     1381 aaccgttcct aatgactgaa tatatgt
//
LOCUS       KCVB01000733            1512 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34769pri ultra-conserved element locus
            uce-142, sequence.
ACCESSION   KCVB01000733
VERSION     KCVB01000733.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34769pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1512
                     /note="ultra-conserved element locus uce-142"
BASE COUNT          499 a          255 c          363 g          395 t
ORIGIN      
        1 ataaactttg aaataaattt ttaaacagat caaagacttc caactgaagc acattagagc
       61 aggaggaatg ataacagcaa aacaaacgct gttctgctac acatgggaat gtgaacactg
      121 tttgaagaaa agacttaaaa aatgttaatc tgtcccttga aagatgctgt tctattagcc
      181 aacactgggt ggcacatttg tgccacattt actatggagg cttcagattt ctattgataa
      241 acaattggtc ttcttatatt acgaacagaa caaaagaggt tatttaatag atttgattgc
      301 catattttta ttaggaaact gaaaaacaaa tttactgcac tgtttttaaa ctattcgttg
      361 atgtgttttt ctaataattc taatttccaa ttttttaaaa tctaacaatt taagaatgtt
      421 tattccatga ggtttgcatg acagttgctg ctggatttca tctatgcatg gcccttcctg
      481 tgggcagccc atagcagatg aggcaaatta agcagtgagg gcaaacagaa caggcaaagc
      541 actgacacac agggactcaa acagtacgat gcagacacgc ttagtcaaga tagagcacct
      601 gacaggcagg ctgtatggag tcagtgtaag agggggtctg acacatacaa aactatgtgg
      661 cttcatgcat tagccatcag agcagcagca gtagcagcag cagaggcagc aacagcagca
      721 gtagtacctg aacagagccc cccagccgct cagctgtgag gtgcgacccc agagtctcct
      781 tattggtcac tggagtgggc tgagactcac ttcctttggg ctcaggcgac ttgacgcaaa
      841 gagcaaaaag ggtagaggca ggtggtcgtg acgtagtggg gaggagtggt gagaaggttg
      901 tgaggggtga ggggttggag gcgtgaggat gaggcaacgt ggtaaaaggg aggtgggggt
      961 agaggtgtga agggaggagg aacaaggagg agggggagtg aatgaaaaca aaagaaaaaa
     1021 ataacaaaaa attatagaag acatctccca agcagaagca ggcttgttaa gacaaactat
     1081 gttaataaag gagtagaggg aagcaaaaat ttatgcaaag cacagatcaa cacactaatc
     1141 aagtcatgca agagtaaaag aaaacagcct tgatcacctt tactagcact gcatattcaa
     1201 tacaacacag acaattatct atgtcactgg acatttgagt gacagtatca tttaaactag
     1261 ggctgatcct ttctggtcgg ttggtggatg tgctcttgcc caactaaatt cttagaggtc
     1321 cgacagtcgt tggtgttgtg ctcataagga gaaaagtgct tcttaatcca ttgttttccg
     1381 gggtggaagg gacacatgca gacctgatgt attctatttt ttagtaataa gctatattgt
     1441 gttagaaata acattgatta taaataaaaa taatattcaa agatattttt acttaacaaa
     1501 tatgtttctt tt
//
LOCUS       KCVB01000734            1720 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34813pri ultra-conserved element locus
            uce-901, sequence.
ACCESSION   KCVB01000734
VERSION     KCVB01000734.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34813pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1720
                     /note="ultra-conserved element locus uce-901"
BASE COUNT          482 a          373 c          434 g          431 t
ORIGIN      
        1 caaatattag acattttaaa tccactctgg aattgatatt aagtccaaca atgggtccag
       61 agacgcagca gcactttacg gaaatttact gtgacaaagc atcaaaactg agccccttag
      121 gttcagatcc atgcatctgg gatccgtgtt cccctcatcg atgcgtacag caattacagt
      181 cctcattatc atcaccacag gaggcatgca cgcatatata ggtgcttgtt tgccctgctg
      241 cacaatcaca gtcatgtaat aatcatatag cacttgggtc agatggaata catcaagcca
      301 tagattaatc cgattcactc caccttacag aacatgtgcc atgcatagct tacatttcat
      361 aacagccaca acagtcaccc atacatccag catatctatc actcaggaat agcttcacac
      421 atcaatgtcc ctcaacaaaa accattcaat atttaatctc caagtcttac aagcttacac
      481 caaggtgagg aaaggaggaa aatgattcat gtaagctaga aaaccacatt caagtttaac
      541 ataaaagccc cagcagtaca atagcagaca gtataaccac tgcagtgcag tactgtatac
      601 agccaggtgc tgatattcct gccttttaga gcccacacaa ggctaaccat tctgggtaac
      661 tgatcaacta cagctgcctg cacgagccca atacaaacag agagagaaat agaaagcaga
      721 gagagacgac cggcgacgag gctggccgct cctgtactca ccagcactgg cagaacacag
      781 cagagacacc aagagcacaa tcaccagcgt caggatcttc acattcattt ctgcactccc
      841 ctctggctct ccgctagtca ctcgctgata tactgtagtg gtgtgtgtat gtgtgtgtgt
      901 gtgtgtatgt gtgtatgtgt gtgtgtttgt gtgtgtgtgt gtgtgtggat gggagggaga
      961 gagagagaga gagggagaga aagagagaga gagagggaga gcagctgagc ggcaagagag
     1021 ggagagggag actgtttgct cccctgagtg tgtgtaattg tgagaagtga gtgtgtcaat
     1081 ttgtgggaat gtgtgtgtgt ggtggctccc tgactgtgtc tgtgtatttg tctaaatgcg
     1141 tatttgtata tcagtgtgtg acagcgccac tccaagaccg aaggcacgtc tttatctgag
     1201 ctctcccacg aaggtgtgtt tttgtgtgtg tctcttcttt tcttctgtca gagaaacggc
     1261 agcatgagcc aagttcagta gagctgtgaa cgtgctggaa gtccgcttgc aggtctgtct
     1321 gacggtgtgt gtgtgtctct atgtgtgtaa gagttggtca tgtggcttat gtatcattga
     1381 ctctgtgggt cacggaaaag cctccccgcc ccccccatcc caccatcacc caccctctcc
     1441 accccttttc cacaaacatg caagtgcaca tgcactgagg aaagagaagg agagagagag
     1501 caaagggagg gaggaaaggg ggagttggta ggtgaagggt gggggtgcat gcaggtttat
     1561 gataataccc aaacagatgc tcttgttgaa aagtctttga attgcaaaca gggaagcttg
     1621 ccaggaatcc aggaatcaaa ataccagctt attaggggaa gtagatgaaa gaagggaagg
     1681 tgtgagatga ggttcatgct ttcctgcttt tgtatcttta
//
LOCUS       KCVB01000735            1627 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34875pri ultra-conserved element locus
            uce-1135, sequence.
ACCESSION   KCVB01000735
VERSION     KCVB01000735.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34875pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1627
                     /note="ultra-conserved element locus uce-1135"
BASE COUNT          499 a          299 c          323 g          506 t
ORIGIN      
        1 ccgtgactgt acgtctagtg cataggtgaa gagcccagag aagtcaacac aaagcttgta
       61 cacaatcaaa atctctgtac tcaatagctt ccatgaaagt gagttttgct ctcttgagaa
      121 acatacaaag tttgagcatc tgttccatca tgtagtcttt gttgagaaat taaatattga
      181 actgatctag tgtaccatga atgtttcact cacatagtga ttagacagac tggagaggca
      241 agacattgca gcactttaca ctctatttta atatactgta tttgttgttc ctgcctttaa
      301 gtgttttata caccagatta tattgttgtt gtagaaatgg tttgaattcg tgctttaatt
      361 aggctcaacg tgcacatgtg attctagtat gcatgctaat acacatgcaa atttagacgc
      421 atgttattta tacgtgcgca caaacacaat catccactgt acacaatgag cattgtgagt
      481 gcagtccggt tacagaaatt agttttggtt ttatgattca gttatcagct tgctggcatc
      541 tgttcttacg cttttatttt tgcatgcatg catctttttc tgtgcagcat atagaaaagc
      601 acctctttaa ttttttttct tttctttttt ttgcagttaa aatctttctt tacttcatca
      661 tccgtatctc caaatcaaca actgtctcaa tggagacagg agacacagcc tgatgatgtt
      721 ttttgacact gacatttcag attagatgag cagaccaagg taacatgcaa gcaggagaag
      781 gaaacggtat catcacagag atcataaaat ggtgtcgtga gtagtctttt gtccgggacc
      841 ataggagagt tctgttgcta aagggaagga caaaagcaga aggcgccacg ggtataaggt
      901 tcccctgagc aacaatgctg aaagtttcaa cttgcagcac atgggtgtaa ctttaactgg
      961 aatactgtgg atcaaagact cacaaacaaa gtgggtggaa atgctggagg agtgtccatg
     1021 ctcgagtgaa ggacaggttc accagagaga aagcgagaga gaccatttat gaaacacaga
     1081 ccaggcaatc aaggctaatt actgagactg ttttgtcaac tcaaagggca gcgtgattca
     1141 attaacaaaa ttgattataa tttttttgct cattttgaga aatataatta cccttcttaa
     1201 aaaaaacatg tggtaaaaca agcttatgag atataaaaac aaaatctaaa tttaattgaa
     1261 ttcaacaaag agatttaaat tttgctactg cttaacagag aagaggatac acagtagatt
     1321 tttgtatcgc aactcaaaca ttcactctac actcctgtct tcgcctgtcg tcttttagag
     1381 agtgagagaa ttattcactt cttcttcttc tttaaataag aaacacagcc ttgaaatttg
     1441 tggggcttaa ggcaactcat caggttagat cagtgcaatg tggatgtgta acctttactc
     1501 tgcagttgaa cctgcacata caagtgttat gaaaggtccc tgtggcactc tgctcttctg
     1561 acatggattt gaagacttgc cactttatta gctgctaagc ccccaatact tgttctatat
     1621 agtggag
//
LOCUS       KCVB01000736            1470 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34918pri ultra-conserved element locus
            uce-859, sequence.
ACCESSION   KCVB01000736
VERSION     KCVB01000736.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34918pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1470
                     /note="ultra-conserved element locus uce-859"
BASE COUNT          432 a          314 c          340 g          384 t
ORIGIN      
        1 ggtcccactg gttgcagatg ttgggtgttg gtaatcctgt gtaatccaat gattatgaat
       61 gattatgaca cattaactct gacctaccgg gataccaccg tgacaccggt gatccaaaac
      121 tcaccaggaa aagccccagc ccgcctcctg ccagccatac tgaatttatt gatggggtga
      181 actgtgaaga cattatcgga tcacttcatt gcctcatgat tgcagcagaa aaggtaaaaa
      241 taattgatac caagagtcca cttaaaaaca cacacacaca cacacacgac cccttttaaa
      301 aggcagcctt aaggttatct cgtttaggcc aaaatgttta gactacatag tgtttctgag
      361 cagcctacag cccgcacgta caattaagat gcacatcaat cctcgttttt ttccacaggc
      421 aatataaatg ctgacaccct gattccaaaa gtccaaaaag aaacgacaac aaaagagtga
      481 aagagtgcga tttgtttgag aaattaccac ttgatctgca taatcggggg cctgggctgc
      541 agctcggtac ccgggggctg cgtttttatt gcgcgtctct tcgccgttat caatttcagc
      601 agcgcgcctg actttttaaa agggggacga ggccggctgc ttctgaataa tgaatgacac
      661 gcttttatat tgtcgctaaa gtgcccgtga tgttacaata tgaaaaggca gcggtaagta
      721 atagtgcatt taaaggggat gtagtgtatt attatgatgt aaaagagaag gggagaggcg
      781 gtagagagag ggagatgagt ggatagcagc acccagagag agagagattg ttttattaca
      841 ataagaggag atgagtttgc ctcgcagcga aatggggacc agttaatgaa actttaccgc
      901 agctaacgat ttcccctccc tcgctctaaa aaccgccctt atgaaatatt taaactattt
      961 aatataggta gtattaagct gaagacactg gcatcggcca tctgacaaca tttacaaggc
     1021 tgtggatttt ttaaagttgg gtaaacaaag ctttacggca cgctctccct cagcccacat
     1081 acaacaaaag tcgtacagta aatcacgctc acattgagtg caatatagtg aaaatgtgtt
     1141 ttactgttgg gagacttgat ttagtctaat ttacactttt ctccatacag agccgtacgt
     1201 cttttcacac tcatatgcag cagtttacaa gctcatccta tcaatatagg gctgtcttgt
     1261 ctataatggg agggatagcc tagcatggcc ccagtgtgag gagggatagt gtttcatatt
     1321 gacatgctca gtgggattgt tggatgagaa gagaggaatg ggatccccca tcagggactc
     1381 ggtcagggcc ttcatcatcc ctgggctgtc actggaccac tgcactgtgc actgtgtgga
     1441 cacaatttat ctgcaagaca cttaaaaggc
//
LOCUS       KCVB01000737            1205 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 34962pri ultra-conserved element locus
            uce-772, sequence.
ACCESSION   KCVB01000737
VERSION     KCVB01000737.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="34962pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1205
                     /note="ultra-conserved element locus uce-772"
BASE COUNT          372 a          296 c          237 g          300 t
ORIGIN      
        1 agataaaatg caggcagcat ttacacacgc acacacacac aaacaaacac acacaaattc
       61 agacgaatga cggtccttac tcacaacgag acacgcacaa gtagccgatc tctcatgcat
      121 acacacacat acatacacgc actaacacca agacacagaa atttcccata tctaaatgtt
      181 aaaggggcaa atccatttgt ttaaggcagt gtgtaatcag ccccctgccc ttggtgtgta
      241 tgcatcctct gccatccagg tcaaggcctc agcttgtcgt cctgctgcct tgggctgcat
      301 cataagcccg aaccatcagc cttgatggtt attgtaacaa agagatactt taacatgcca
      361 ttcatccata tcaattctct tcccccccat gaattgaact aataaggctg tgcctctgcc
      421 ctccagggag gctcataata tccattgcag atatgaaaaa aaagaaaaag aaagacagag
      481 gctctggtgt ttcacgttcg actttccctt cagactaatc ggcgcgccac agctcgttag
      541 gtccgtgacg tgccggagct ttaccgataa gcaggggaga cacggctcaa ttacccacag
      601 ttacaatcgt tctctctctc ttcctgcgcc tgatgatgaa gagataacat gtcaatattt
      661 gatacaaaca acttcatttg cctaatggtg ttcctggata ggcccatcta caggtccaga
      721 agggatggga gatctaagaa aaggcctgga gcaacactcg gcttagacgt accgtagccc
      781 accactcctc aaatcacgca cacccattcc cgaaagctat gcagaaggca tgctgggata
      841 gagcaaggtc agacatgaca tactttggca acttttgctc aagttacttt cagctggttt
      901 ctgtgagatt tgacgatcaa ctttgaaagg ttaaagagga ggcttagttt tgacatctct
      961 gcactcacat tgctgaatac tggatgactt taggtacaag aatggtatga ctggaaattc
     1021 aaacatttgc cacaagacaa ttagaacccg tatggtagga atcactttac actgccagtg
     1081 atagattaga tggaccagaa cttcaggttc aacatcaaat gatctcttat taacaaaata
     1141 tcggacaaat ccatggacac aataacacat ctgtatatat gtaactgttc tttcaatgtg
     1201 aaatg
//
LOCUS       KCVB01000738            1340 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35007pri ultra-conserved element locus
            uce-737, sequence.
ACCESSION   KCVB01000738
VERSION     KCVB01000738.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35007pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1340
                     /note="ultra-conserved element locus uce-737"
BASE COUNT          417 a          296 c          298 g          329 t
ORIGIN      
        1 gacaaaacaa gcctccagga atactgggca aacactgaga gcctctcact gggatccgca
       61 ctatttcctg agagcaccac agccaaaaag cttcacactt ccaatactgt cttcacttct
      121 ttttctaaat tgaattgttg cttccaatat caaagatatg gaatagtcat gatcagagaa
      181 tgtctgttca gtcagtggct tgaaaatatc agcacggcgt cactctgcaa ggtgctgaaa
      241 ttgcaaacaa acgtgttctt tcagcacgct taaacaactc gcaacaagaa ataaggtcaa
      301 gcatgttgta agtgattcca cctcttctca atctgcgtgt gctctcactg tacgaggctg
      361 tcgaggctca gccaggagag atgagagaga tagtggaggc tgaggtagga accattttca
      421 cactgccaca attacaggaa ccgtcattac attgctcact cttactcaaa tacagataga
      481 agccccaaaa aattatgcgc aggctaggag gagaaagtgg ctcacttctg tttgctaaat
      541 taatcgcttc ctaaatggct ccaggccagg caaagttaac atttgcagtt tcaaaggttt
      601 tcagcgcttg caatgagcca tgaaattact gcatgccgtc gaaaatggca gctgatgaaa
      661 agcccaagga gcaggtccca caggcagaga gagcttggaa gtgaaatctt aaacacaaag
      721 tctcatttct gatttggatc atggtgaaat tcagaaccaa aactcgagaa tgtacggacc
      781 catgtccgaa agcaggacag gttgcgacaa ggcagatgac gaacagctgc tcgcggcctg
      841 gcaggctcat ccaggctccg aggaggcgag gtgagcacag aggttgggtt gaaggagcgt
      901 aatgatgatg ttgaggtgct cagctgcagc caaaatggac tgacccaaat taggtttgat
      961 taaatcacag gggtgatgct gcacttctgc agacatcatt aacatcaaaa tgattatggc
     1021 ggagcagttt ggctcagctg aacccaacca attcacacca agagtttgat gctggaaaac
     1081 atttgcatat tgactgaatt aagctgtaag cacggccaca ttgtaggatt tgcacctaca
     1141 gcaatgcaat tcattcttca aatgagaggc tttcgtatga gatcattgca agaatccaaa
     1201 cagccaagtg aacacaggtc agttgaatgt tttttcccac cgaaaattag acataggtta
     1261 gacaagaaac aaatatcatc cctcctcatc aaaattttaa gatcacattt gccaatttct
     1321 tgacttttga ttcaacattc
//
LOCUS       KCVB01000739            1379 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35086pri ultra-conserved element locus
            uce-1090, sequence.
ACCESSION   KCVB01000739
VERSION     KCVB01000739.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35086pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1379
                     /note="ultra-conserved element locus uce-1090"
BASE COUNT          398 a          287 c          240 g          454 t
ORIGIN      
        1 atcttacgaa gcattctctg ccaacttaag ttgttcagct gagttttcag ttctctgtaa
       61 tacagaaata atttggaatt tgaaaccaag gtcttttgaa aactgttctt tgctattgaa
      121 aaatggatga tggtgagata ttcagtattt tagtagaaac ctggaaactg gcagagctgc
      181 ctcatacttc acaagccctg acaaaggggt gtacctcaag gtccaacctc aattacatca
      241 ggatgcttaa atgcaagctt atacatgttg atgtgttcat ggtgtgtatc aaccatttat
      301 ttgtaattat tttgcatttg tacttgaatt gcacatcatt tttctataca ctgggtattg
      361 taaagggttt aagatgcatg ctaactaaac catgaaatct tttctaaacc caaatcaatt
      421 caggcaatgt aatcaaaggt gctattcatt gattttaaag gcaaaatgag ctaaagtaaa
      481 ttctgctatt gctgcaaaag tcacatgttg cattaaagga gtctccattc ctcccaccct
      541 ttgagggaat tacctcacaa gaaaaaaact ggatgttgta tttcacaagt tctgcttgcc
      601 gaactaaact tggcagtttg ttagcttcgg tgcataaaag ccaactcttc acattctaga
      661 tctgcttgtg cacctgagga cgatctgtgc ccattttatg tgtgagtatg actaacttcc
      721 acgccggcgg tgtttcggca aaggagctgc catctcggtt tccttcctct cgttgcctcc
      781 atctgccgtc ggcacttaaa cgcagtctgt taaagagctt tatccagaca cagaattaga
      841 aagtcaaatc attgcagccg agtttctaaa cagtgaagac aaccaaatat gaaagtttgg
      901 gattttaatt ggtgaagaca ctgttctaaa tatcattgag acatgttgaa taacatttga
      961 ttattctctg acgctacact ccattaacca acatacattt caaatttgtc ttgtggcaac
     1021 aaactgaaaa tagcattagt acatattcta acacatataa tcaaagtaat atctggataa
     1081 atcttgactg actgcatcac ttactctcta cttaactcgc tgcataactt tttttgaact
     1141 aacatgttca ggtattacct gtgtgttttg gacttttaat gtctcacttt actaactaac
     1201 tgttttcaca ttaatcactg gtctctatgc tccgccatgt ctctcactgc atgttgtgat
     1261 gtccctaatg aagtcactca tccatttcat tacttcactg ggattcattt tcctctctgt
     1321 ctgcatgccg tcacgtggaa aaactaaatc aacccatctg ttgaggatct gtcagcttc
//
LOCUS       KCVB01000740            1219 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35124pri ultra-conserved element locus
            uce-473, sequence.
ACCESSION   KCVB01000740
VERSION     KCVB01000740.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35124pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1219
                     /note="ultra-conserved element locus uce-473"
BASE COUNT          376 a          268 c          281 g          294 t
ORIGIN      
        1 cgagttgatc aaccataatc acagagacga gttcagtata aactgcttca aaaccgttag
       61 ccgataaagg ttgctaatgt gttcattcgc gttcaaataa acttaccaat ttgacagtta
      121 aaagcagaaa aacacacaag caaagcgctg ttgtgtctga gctgcccgtc tcaacggttg
      181 gtgtcaccac agcgccaccg tataatgagt cgcacttatt tttcccccaa attatccaac
      241 ctacaacggc taaagcgtcg ggctaactac ggctactcgc tccagtaaaa gttgttgaac
      301 acgcaataaa gatagctaac cgaaagcttt ccgccaaagc cttgctgttt tgtttttatt
      361 tcggcgcatc cccgcggcgt cgccccggct tgcccagttg cagtgtccga tcgatgttga
      421 attgaacaaa gaggatcaat ttctgatcag cgagagagcc actttcatca atgtgaggaa
      481 gaggtcttga attctcttcc gaaacaccta cgaggcggcc gccgaaaaac acaaaacaca
      541 gcggccgaga gagacaagac agccgaagcg aacagggggt agacgaatta aacagaggtt
      601 ggactgacct gtagttgttg taagtcaaag cgcttccaat attgaaacat cgatcctgca
      661 ttggccgcca tcttgagaca tattgagaca ggaatgagat gctgatagag agcgcggggg
      721 ttggagttca gtggagagga ccgggcggcc ggaacacccg gaccggatca cttccactac
      781 tcacctccgg taacagctgc tgtacggaac ggagtcagcg tacaggagtg ttgccttaat
      841 aacgccatta taattgctta aatagctttt ttatcaacat tcagatatta caggtaaata
      901 accaaactgg cattaaatta cttttcattt tattacgttg aaatacacga aattgtgtgc
      961 gccacgtgag cccatgttgg attttatagt ttgtgtgatc ataatgtatt ttggagagca
     1021 aagatcatgt aaaaataatg ccaggaggct acttggaagc catatctaaa aagaaaaaaa
     1081 aaaactcacc aggggagcat gcaggaccaa tgtttcctct gagctgtgtg ggactgagca
     1141 agaggataat tcagacctga aaaagatctt acatcagtaa tcagtgccac tccaaaatca
     1201 gcaggaaagt tagatttag
//
LOCUS       KCVB01000741            1607 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35210pri ultra-conserved element locus
            uce-512, sequence.
ACCESSION   KCVB01000741
VERSION     KCVB01000741.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35210pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1607
                     /note="ultra-conserved element locus uce-512"
BASE COUNT          491 a          335 c          347 g          434 t
ORIGIN      
        1 tattagaact tgttcaagct aaaactgtat atcacccttg tgcataaatg gcaatagaga
       61 cccactaagc aacattaatc gagtatttaa actaaattta aaggatatgc ccagtgtctc
      121 atttgactaa ttcattgatg gtgtatctcc acctgcttcg gcctctgtgc cagtgtctga
      181 aattgtgtga cggaggggct gaatggtgat gtgtgacaca ctcctgatga aaaataacag
      241 cggccctcag atctgcacaa cggctgatga agaatcactg acttttattg gtgccgaata
      301 aaaggatgtg atgaaagagt caaaggctga gagagagaaa gagggattca gagggagggg
      361 cggaggagtg caaaacaagg ccccatccat cacatgagtt gagaagacac attccagtgc
      421 ccttagcgag aggatacatt cactcaaagt cacaatagag ggggaatctg ggagaagctg
      481 ggaggggcac ccatcaatac acaacctcac ccttgggctc tctgtgcgca caaactcaat
      541 gccccttcca gtccaattca attccctcta taagcatgac aaatgtacat tttgctgccg
      601 atgaatgcgt tcatactcaa aacaacaaaa cagacgaaaa gaacaaaatc ccggcatgga
      661 tgctaatagt taaaattctt aattcccctt ctcttctccc tccttctctc agtccctctc
      721 tctttccctc tctctctctc tctctgtctc tctcagtagt cggagttgga aaaatttgag
      781 ctggggttgt aggatatctc tgtctggcag agttagtggg gattttaggg tggagggagc
      841 aggtgatggc tttctggagt gcactcattt acactcctcc ctttctctcg ctgagcagcg
      901 tgaagaggtg ggagagatga ggggccgcct cctctcactc ctgtgagctg cacctgccag
      961 gggtgagagg atcaattgtg aactctgacc ttgtaccttc cacaatgccc aattattacc
     1021 aataccatgt aatatataca tacattagag atgaacaaac acgttaaaat tctggtgtta
     1081 aaaacataat tgtgcagctg cagaaacaat cacaagtgga gagcaaatag gatgtaaaaa
     1141 aggaatatta gactgacaat aatgacactg ttggacaaga taaacattca tttagatgct
     1201 aacctgttgc ttgcatcata aaattaattt ttttgtgatt ttggcttgac tgcttcactc
     1261 ttctgcagtg tcactgtgtg agatttttca gtagagtttt aacttgtaca aaacccaatt
     1321 attacctgta ccacgtttac aaaatacttt aacagaatat acattttctg gcattgaact
     1381 cacattgttg tgaagctgca gaaacacctt taacaagtag agagcaaaga tgtatacaag
     1441 gaatccggtg atttattctc cattgacaac ataaacatga atcaagatat cctctgtaca
     1501 gtaaataaga agctatctgc agcaaccggt tatttaagct gggcaaaaag actggaaaca
     1561 gggggaacag taagtgtggc tctgcaaaat aagttgtcag gcataat
//
LOCUS       KCVB01000742            1445 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35257pri ultra-conserved element locus
            uce-980, sequence.
ACCESSION   KCVB01000742
VERSION     KCVB01000742.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35257pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1445
                     /note="ultra-conserved element locus uce-980"
BASE COUNT          381 a          357 c          265 g          442 t
ORIGIN      
        1 taatctatac ctacaggaag tcaattaaaa agtgccaggg gagattaaga gcagcgtgac
       61 agcttttcgt caggccacgg gttcgtctga gagaaccagc gtgtcgcgtc tctgctgtga
      121 gtgtgtgaaa ggtaaagtgt gtgtgttggt agtaaaagac gtgttgcaaa gcgggtggcg
      181 gcggtggtgg agggaagaga ccgttgccga acacccttaa aagccccctc atgaatatta
      241 aaaaggcctt tggcagccgg cagctctgct acaagtttgc aaaccagcgt aatgtgtctg
      301 tttgcgcagg gaaaggactc cgtccggata tcaattaaat caagcgttgt cagtctgatg
      361 gccctctcaa tggagggaca gcccattccc ctctacacat cagctcaggt ttgtgtcaac
      421 catccatctc tcatcctccc cttcacttcc acctctctcc cggttgccat ctttatcagc
      481 tttgactctc tctctttctc tccctctctc tcctccttct ctcctttgct ctctctccct
      541 ctctgtctct caagcagccg gtaactgaat gcacaaatga ctctcccctc cacctttaac
      601 tcccactaaa tctttcccct tttccacttt gctgagccgg gatattttat tcctccatca
      661 aatatgcatc ccattaatta agttaaatta ctcctgactg ccaaatatcc cctgccccga
      721 ttggtgtcaa catagaaatg catgactgtg aacctgcaat gacacggccc caacacttaa
      781 acatgagtta cagccttttc cagctccatc catgggcaga ttaccagaca ctttgggctg
      841 ctggaattaa gcaccgtttg ctccttcccc tccacccagc tttttaataa gtgcacaatg
      901 gatgacactc gcacttctga caacgagagg actgatattt taattacacc ccttttttca
      961 tctgccttcc tcttcatctt ttaattgctg ttgttaatca ctcattaact ttcccctctt
     1021 tatttttttt tctcctacaa tatacctatg acatgattag ctctatagaa tggattgttc
     1081 agcaacattt ttttaataat tcaactactt taaaggataa agtttgtact tcttttgtgg
     1141 ggacctggta tggggtggag ggtgcaaact taactcatgt ttctctatca aatgaagcca
     1201 atttgctttt cttagctgat gttaatcttt agagcataaa ttcaaagtgt gtaaatgcta
     1261 tcaagatagg gttttacaag gcaaaaattg ttaatactct attctaatta ctactacttc
     1321 attttgttct gcgagtccag caagttgaga tttaacgaaa acaaaaaagc gataaaaaaa
     1381 agctgagatc acaagaagtt taacatttaa gaaaagaagc tactgagatc atacatagtg
     1441 cacac
//
LOCUS       KCVB01000743             852 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35274pri ultra-conserved element locus
            uce-557, sequence.
ACCESSION   KCVB01000743
VERSION     KCVB01000743.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35274pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>852
                     /note="ultra-conserved element locus uce-557"
BASE COUNT          268 a          182 c          188 g          214 t
ORIGIN      
        1 gtgtgcaaaa aaaaaaaaaa aatctcagct caatttaagt ctcatccaaa gagcttacaa
       61 ctgtaattaa atcattaaat tcttgtctac gcttcacaga gcgtagagaa attaacttcc
      121 caccaacctc attttctatt tgaacatgaa ataatgattt tctgccagtc taattacaaa
      181 gccaacatct gatcaagccc gcatccagag gggattatca catgcgccag tattagacct
      241 cattaccagc ctgagtgcag aattattaca tcttgtaatt ggatggtgag atttatatac
      301 agatcgggcc ggtttctgta agattgtaat tacaagcttc gttggttagc tacttatcag
      361 aactttgcag gaaaacaaaa caaatggctg agcgaaatga gcatgtcatt aacctgtaac
      421 cccagcgtgg ggggagaagt ggtgtaacgc ttgtgtctgc attaagggaa ataggttcat
      481 ccatttcagt gccccgcctg tgatggctga gcaaaacagt ctgtttcatt tatttaacct
      541 gggtaatctg tgtgggtaga aaacacacgc acagagagag agagagggag aagaaaaaag
      601 gctataaatg agctgtctct tctgccagga tgggctcaat gtttctatta cttaaggcta
      661 acaggctgtg aagccatcca ttctggaggc tgcgaggggg ctatttcgcc tgcctaggag
      721 ggagaagggg gccatcgatc ccaaagcccc tgccgcttgc ctgcaagagt acaggggctt
      781 tgttgcatca ctaaaacaaa atagagaggt aaaactcaca catgcactca cacacacaca
      841 cacacacaca ca
//
LOCUS       KCVB01000744            1711 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35311pri ultra-conserved element locus
            uce-938, sequence.
ACCESSION   KCVB01000744
VERSION     KCVB01000744.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35311pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1711
                     /note="ultra-conserved element locus uce-938"
BASE COUNT          473 a          322 c          362 g          554 t
ORIGIN      
        1 tcttttaatg atattaatgt acgttttgaa tttatcacat acatgtgtct gagcctcctt
       61 aaatggggct ttttaagcag ccacgaagca tgaaatgaat tttcttcagc cttttctcta
      121 tttaaaagat gtcttgtgtg gagctgtcca tgctctccca tcagctcatt aaaatgtaat
      181 gctgatgctg gttctatgga acagtgcctc ttaaccaaag ttcatcacca gggatctttg
      241 gtgggggagg gggggggaaa ggggtggggg gtgggggggt aaggtgaggt gggggtgtcc
      301 tgggtcctca tgtagctgcc tttctgcaac catagcgctg tttacggatt tcttaaagaa
      361 aactgaagat ttgtagcaga aatggagata attgaaatgt taaatataga aattttatgt
      421 gattatttgt cataacggcg ttttaaaata agagtaataa agccattttt tttaggcgtc
      481 attatagtgt tatatcaata attactacag caagatatca cctcgaactt taacgcaatg
      541 aattaaatca ggagcctttt tgacaaatag gaagtcattt taaatcgcca acacgaaaag
      601 cgttaatttt cttattttta tttattgttt atcaccttat tttgtaatgc aatatttttt
      661 ttttaattac gttcatgcat ttctgcaaaa taaactcgtg cattaaagcc agtgttgctc
      721 tctgtgtctg tgcagcagca gcagcagctc cagcacatta gatgtgctaa agaaaattgg
      781 cctgctttat agcgccacgg gatctggtca ccgaatattg atcggttttc atctcgacag
      841 attcactcag tcctggatgt actgctgatt gttgagggag tttggtctga aagattgaat
      901 tgcacctctg agaccgcagg cagcctcggc gtgccgggca gcacaaagtt ggtcaaacaa
      961 tattctgttg tgggtggtgc gctcagcaca gatatactct gatgaatgat gttcaatttg
     1021 cgctcagccg gccctcttca ttaaagtgtt tacatcccgc agcccgttgt ttaacccggc
     1081 tggattatac aaaaggaaaa aaatctgaat atcctgaatt tgtgtctgtt tttaggttat
     1141 gttttaattt ctgtttccta cctgttaatt gatgagacgc tgggaacagt gtcgttgtcg
     1201 cagatgccct ctgccagcag tctgtctctg atctcccacg caaacatggt tgggttttgt
     1261 ctcttgtagt ctgcgatttt ttccacgacc tttggcgtgg ccactttggg cttagacccg
     1321 ccaatcaccc ctggtttgat gctgccagtc tcgtagtacc tgcaaaaaaa attatgttga
     1381 cgaattagat gatagcttgc aatatttaaa acgtgataca gccgatattc taccctcctt
     1441 attatagacg tttaatgtca ttttaaacga agtgatacat ccagattaca agctttggag
     1501 agttactgtc tgaggactat cttttattac aagaaagtgt aaaaacgcca aactttctgc
     1561 agcaaaacat cacatttaag ggttttatga gtagtacaac gttaactgac gtttcaaaca
     1621 tttcaacttt tcttaaaaaa caattaaaat gcaagaaaat tgcattagta catgcataac
     1681 tagacgacaa acatgcattc cgtataattg c
//
LOCUS       KCVB01000745            1615 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35350pri ultra-conserved element locus
            uce-613, sequence.
ACCESSION   KCVB01000745
VERSION     KCVB01000745.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35350pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1615
                     /note="ultra-conserved element locus uce-613"
BASE COUNT          392 a          329 c          406 g          488 t
ORIGIN      
        1 atgaagaaag gtggacggtg ttaaaagtca ttcttaagtg tggcactgat gataggaagt
       61 tgttcttgtg agggggggaa atggtgccat acctatgtaa cattcatatc atttgaatta
      121 tgaatcagag taagaagaac tctatgttct aagctgatga atatttaaag gattgagtat
      181 acagatcatc atattgacaa agggagaata ctaatgtcct cttcttggag tctgattaga
      241 ggttaagctg aatgcaaact gcataattta ttttcattct tgcatttttg taccttttga
      301 ttcgactcct ctttttgggt ttgataactt ctgtgtgcag aagtctgtcc ctttttaaac
      361 tgaaataaag taaagtgctg catgcaaatt agggataatt gctgtcattt gtttttagca
      421 catcgaagaa agttaaggat gtatttcggc tgagaaaaaa tgttttatgt gcagcagacc
      481 tgcagggtaa cattaagtag aatatgagtc ttccgttttc gaggccagtg cttcagtagt
      541 tgacatatac tgagtgccaa ctgtccccac tgacacagac attgtccaca gtctattaaa
      601 ttgaatgttg tatttaactg gtttggagcc agggcaaagt tacattcttt tgtatcattc
      661 taacttctct ctctggtgga atgtgcttat ttcagggaga gacaggcaga gagagaaagt
      721 gggagtgaga gcccatgcag gtgagagtgt ctaacagtga ggaaatcaat atgatgtaaa
      781 ttattaagag ctcaggaatg cgtctgaccc ctactgtctt gcagcaggct gggctctgaa
      841 ggctggcagg tggacgcgag cttggactct ccctctaaca ctcattagtg gctgggagcc
      901 ggggctcctt gtgagtggga aggcagggat cacagcgggg aagtgatggc ccaggcctct
      961 cacatcccgg ctcagccttc gctctggctc tgccagcctc tccctggaag ctcattagta
     1021 agtgcactga tgcataagag ctagtcatta gctgggcttt ttttccgtct tcatctctct
     1081 ttccctcgac accatgtctg atcctgaaga ccggtggcag tgctggttta aaagacgaga
     1141 gaggtccaca ttaccgtagg tagtctagac atatggatat gagacagtgg tagccatcag
     1201 attttttttc ttttcttata gggggtgtag ggtgggggtg tcaaattgga gttcttcaaa
     1261 tggagtccat ccatagccct tcaaaccaaa gagtaaacat tagcgcttga ccaggacttt
     1321 ggctgagcgc ccctttcttc ctccatggtt ggttttgatc tggggtattt gcttttgctg
     1381 ctggccagat cccctcagca cctgatggtt gcctaggctc tgagtaaata agatctgggg
     1441 cccaggcctc ttcggggctg ctgggtaaat aaggtctctg gtgtgtgtag cgtgcctggc
     1501 agcagggtga gcactggtgt ggaggcctcc agagacacgc catccccctt ttctatatct
     1561 agctgtttaa ttctgttatc ctccctctct ttaactcact ccattctccc tccct
//
LOCUS       KCVB01000746            1523 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35389pri ultra-conserved element locus
            uce-287, sequence.
ACCESSION   KCVB01000746
VERSION     KCVB01000746.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35389pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1523
                     /note="ultra-conserved element locus uce-287"
BASE COUNT          481 a          243 c          337 g          462 t
ORIGIN      
        1 attcccaact gagtcactct agtaagatgg aaaagcacaa ctaaaacaca acattcatca
       61 tctacatgga gcagaggagt gatcatacat gtaatttggt gtatttacaa atctgattat
      121 tacatattga tttaatttaa catgccaaac ataatgcgtg tgacataaaa gcacatcatt
      181 ttacatttca catcacatca tggacactga ggggagaatt tattctgatt ttttttggca
      241 tatacatatc cctaatgatg gtctctggtt aatctgtgaa gagtcgaaca ttgtgcagcc
      301 tgcatgcttt tctctctggg atcatgaaca gctgcttgct cataattatg ttgtcattca
      361 ttgttatttg gaagaggaaa ctcatactga cagctaggat ttttttgtgt atttctgtac
      421 actgtatatt tgctgcttat acagtaatgt gaactgcttg agaaaactca attttagctc
      481 aaaggcattg ttattaccac catttccaga agaatatcaa atatttgtgt cttctatctc
      541 tttgggacat ttgctaccag aggcggtatg gatttgaaat agatttaaga gccacaaaaa
      601 atgaaagcag acaaaaaaaa aggaaaaagg atatggattg agatggagaa gtagcaggct
      661 gattacaagg tgtcaaaatt gccaggagca agaaggtgaa agcaatcagg ggtgagaaga
      721 gtgcagcatt cgtgaggggc aactaattat ccatctcatt tgtggaaaga agcatttgag
      781 gcagctctag cccactgaat ggtgacagat tggtgtggag gagaggggag gtgttagaac
      841 aatggagccc agctcaccat cattactgca tgctaatcag cagtgcctct gcaccaccag
      901 tcggtgctga gtttggggag acagggaagt cattggcttc aggggaccgc ggtcattttt
      961 aactagggag gagagagagg aaactgacgt actgtatagc tgttggcact ctaacactgc
     1021 ttatttgagc ttataggtta tcctatgagg agattagtgg atgagagatg gcctgatggt
     1081 actggagtgt ttttgagtgg attgattatg gctgacacaa tatcacattt tcactacagg
     1141 attatcggcg ccttgagaat tcatgataac gatattattg cgataatctg tagacatttt
     1201 gaaaaaaaaa tgctatcagt cacattcatt tttaactttg tttactgtgt gttttgtctc
     1261 ttttttgctg aatgaattac actaaaaaaa aaacaatgta gagaggagga gagagagtct
     1321 gcaaccataa ctgtacaaaa gcatacaaaa aaaaaataac cacacttcat atatttttct
     1381 gttttatggc gagtggcaac tggcgttagg gtacattact gccacctact atgacaaagc
     1441 gagaacggaa tgtagattta aaaggagatg tgttattttt attatattat catgggtgta
     1501 tttttaacat tatatcaata ttt
//
LOCUS       KCVB01000747            1544 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35434pri ultra-conserved element locus
            uce-656, sequence.
ACCESSION   KCVB01000747
VERSION     KCVB01000747.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35434pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1544
                     /note="ultra-conserved element locus uce-656"
BASE COUNT          467 a          320 c          302 g          455 t
ORIGIN      
        1 gcctctgcat attgatttgt ctgttgtcta tgcgattaga aagatgtctg acattctctg
       61 tatttcccac ctaccactgt ggggttctgg cttggacaca aaggcaaata tatgacaaaa
      121 ccttcttcag aactgcttac ctttatggtt cactgctgta attggcccag ggtgaacata
      181 cccactcgac actggcagta atgtatttgc gaagatattg aaaaagctga tgagggagac
      241 acaagatgtg ttgtagaata caaaagttaa taatggtggg gattttgacg aatggatctc
      301 taaatgtatc ctgatatcgg tctaaattgc attcttttgc tctcagtttt ttgtgttttt
      361 aatttataca ttctctcatt gactatgcaa ggtgctcgtg cgatggaaga gaacagttca
      421 ctttgtgtgg aggataatgc atgcacaaca agtgctgttg tgttatgtat acataaacgt
      481 ccatggagat agttcagaca acactatgaa accttccaat actgtcatca cacaggcaca
      541 cgctgaacgt gtctaaaaga gtcatggact caacactcat acaatgtgct tttgagtaca
      601 tacatatcat tttattgctg catatatcac ccccacactg agacatctga aaagagagca
      661 tgatctatac caaaacatta atcatcattc attgtatgtt aataaattca gaatttctcc
      721 atttaccatg cggacgtcca cgcgtgctgg aaatgcaacc tttccaaagc tcataaatca
      781 cacacactga gtcatctgac aaagtacccc aaacacaggc tgaggcgcag cgatccctgg
      841 ggcacaaaca cacaccatct aatgtggcgg aatcagagca gggaccagag ctttctgacg
      901 cctcttcctg ctgccaaccc ctaccaacaa ggagcattcg ccgcaacctg gaaaaataaa
      961 aggcagaatc agaggggctt gtgggatgcc cctctctgtc tctgtctttc tagttttccg
     1021 ctgtctcttt cctcgctgcc ccaaggggtt gaggccatgc gcaaacaaca acacttacac
     1081 acagagggtt ggtttgtgtt ggatacagtg gggaccacct gtctttgctt tattagacat
     1141 agttcacaat acaagaaaag taaaaagggg gtttgtgtga ataatgttac ttgcagaact
     1201 ttcagttacc tggttgtgtg aaaatatgta atctcggtat catttttaaa atactaaagt
     1261 aaaaacaaaa caaaagaaaa aacatttaac tagaagtgcc aaagagatac cagctatcac
     1321 cagagtatca atgtcacact gtgaaacttt atttatagct gctgtttgtg acgcacttgt
     1381 tttatgcaga ggaactgtaa tgtttgtcaa ccataatccc gaaactatac tataacaatt
     1441 catctatgct ttaaacattt cggataatac attttttgta gttctctttc gaacacagat
     1501 tttaataaaa gagtttgaga tgattttatt cagtttgtgc ttca
//
LOCUS       KCVB01000748            1721 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35478pri ultra-conserved element locus
            uce-8, sequence.
ACCESSION   KCVB01000748
VERSION     KCVB01000748.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35478pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1721
                     /note="ultra-conserved element locus uce-8"
BASE COUNT          510 a          319 c          375 g          517 t
ORIGIN      
        1 taaagccatg tcccttaatt gtattctggc taagtgggat gaatggtgct ttttaatcag
       61 actactgacg ggggctctgt gttgtaaaag tatagagatg gtgcctgata gttaagatgc
      121 tgaatgatat gcttttagtc caggtttccc agcttctagc aatatttaaa tgtattctgg
      181 cattaagctg gcacctgtaa acattagtgt gatttcccct ggtgtttctg ctactgcagc
      241 aaacagcagt ttggcaagta attgtacaaa ataacgtata gtaatgtcat gtcataggca
      301 gacatacagt tattgttttt ggatgtactc ttgtttaata tgcagtttta tgttcagaga
      361 gaaaagcact gcctactgct tgaatgaagt ttcattgaac agtgagacct actttgttgc
      421 tgagatttaa ggctttgctc ctcctagtgg ctgatcttgg ctatgttttc ccctcagagt
      481 tgtacacaaa tgggtttgat agcagaagtt taacacatca cataataaat tgtaaccaca
      541 tgttttattc tcttccttgt gtagctcctg agatggcgtg tggaaaatag gaattattgg
      601 aatctttttg atgtaacctg acaggctcac catgtagaaa gtaataggag ggatataagt
      661 cctgctgttc acctagtcaa tttgaatttc atgaccaatc aaatgtagac aaggtatcat
      721 ttggtgtgta gggaagagga gagagtgaat ccctttccca aacagttaat ccaactttaa
      781 tcctagtcat taggaattag acatgccgag ctgtccggcc gtgcctctaa attacaatca
      841 aacggttacc aattacacaa attattgatg gtagattctc ttttcaactg caaattaagg
      901 ctattaagga aggctccttt ttaatgcaaa agtgactaat taaccagcgg atgggaaatc
      961 tgctgcccag atatcaaaaa agttctcagc acacattgca gaaacacaag ggaaccaatt
     1021 agtttaatta tcaaaacagc taattaaaat tgcacagttc ctaattagtt ctttgctttt
     1081 gcctctaatt gacagcatgg agataaatga gctgccgatg aaaatgggga gaggagaaaa
     1141 acaggagaaa cttcggcgga atatttatgc tgactttatc tccgtctgtg tggcagccct
     1201 tgaaagtgga aaattaatga ccttaattcc caaactgttt ttcctctaac ggtgctcccc
     1261 tctgtgctcc tggtgcacgc tcctttgtat gggcctgcca ccctgccaac tgctggaatg
     1321 ggaaaatagg ggtacttggg ggtgggaagg agccacgatt ctcctcctaa tgtgacagct
     1381 cccacctaac aatggaggaa gatgaaagaa tggagcagat gtaaccactg ggagcattgc
     1441 atcatgggac tgcaactctg ttgcactttc tttaaagcgc ttctgtcatc tacaggggca
     1501 ctaatttgtc ccatgaaaaa gagggaactc tgccgggaca gagataaaag acatttgatg
     1561 gcccagaaat acttttaaaa agtttttaaa tttgaatttt agatggatta atatgatcct
     1621 tgggaagtga acttctcttg aaactttgca caagcacgta tttgtagatg ttaccaagtg
     1681 gtctaaagac tgcaaaaaca accaagaact tagtgatgta t
//
LOCUS       KCVB01000749            1692 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35517pri ultra-conserved element locus
            uce-860, sequence.
ACCESSION   KCVB01000749
VERSION     KCVB01000749.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35517pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1692
                     /note="ultra-conserved element locus uce-860"
BASE COUNT          485 a          389 c          314 g          504 t
ORIGIN      
        1 agaagaaaaa aataaataaa gacatactag caaaaaggac acacacagag caaggaacta
       61 gtagcacctt ctgagctgag cagagatgag agtgcccact ctgaagtaat tggagccagt
      121 gctaagcgcc agtcctggcc ggtaagtgtg cgggtgtgag tgctggcatc ttgcccctgt
      181 gtcactttca accttgcaag ctgctttgac gataagctac tgccgagcag ctccagcact
      241 gcccattagc acgaaaagac aacagcagtg gcactgatga atgcagtttg ttgtcagaaa
      301 attaaggatc tgtatttagc tgacttctcc acagacaatc agaacacagg ggaggcagtt
      361 tttgtggata tcctttcgct atagggcaat agtaaaattc cagaccagtt gttagacgtt
      421 taatgagtca gacaaaatca cgagttaaaa ggagcaccag ctagcaggca ccagtctgta
      481 acaatggcat ttctgtcagt catttttacc aaattttcac ttaaaagaac ttctctgcag
      541 catccccagt tgccaaattc ttaaagactg aggaagtgga tgatgtacaa gcatgagcca
      601 gcaattgatc tatcggcgtg cctctctaac ctcttttcat ggctggcatc actgccgtcc
      661 caaagccttt tacccattcc cattcccctg cctggcccta tattggcccc ctccttcttt
      721 ttgctttttt gtttctattt ggttcatttt tgctgtccct tcctttcttc cctttcagaa
      781 agtgatgcac actcgcaaaa gacattctga gctgtaccat gagctcaacc actcgtccaa
      841 gttccacacc atagacaggt atagcaggga cccagccatg tccacattca aggtaagatc
      901 attgcccaaa cgcgggatgg gaacccgttg tacccatgca cctccacccc ctcccgcaca
      961 tggtcacaac cctaaatccc catcccttta agaatgaaga tttgacactg gaaagcaaac
     1021 actggcctct gcacagatca aaaattgtag tagtatgctg gtttaatact taatacttcc
     1081 agttccatag tgacttccca tttccttgta aatgcagcat tcagatgatt acagtcataa
     1141 aaatttggaa taaagctccc attcatatac aacataaggt acttgtaaaa acgttgttca
     1201 ctctagcaag caaacttgga tgtaatgtga ataatgtatg tattttcatt gtcaatagat
     1261 taatttactt ttgtacttta ttattgctcc cctggagaaa cgaacaacaa aaactacctc
     1321 ataaataaat ctttagaggc acattttatt cctgctttca ttttgtaaat atctttttat
     1381 acctgattta atttgcctga acttaattta ttgtcacttg cggttggtgc cttattagat
     1441 acttgtcaaa acagttcaca tgagctgtta actgaggaat aatcaatcat agctggttaa
     1501 ttaaatcaga attctgaatt ttaagacctg tgtaactatt tctccctctt accaaatcac
     1561 ccgaccagaa tgcactgctg aaaaaacatg tgcctataca cacaagtgct ttgtctatgc
     1621 ctgtgtctgt ctgtttaatt ttattatttg tcaatgtgtt gtacagtgtt ttttccatgt
     1681 gtctgtgggg gt
//
LOCUS       KCVB01000750            1490 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35561pri ultra-conserved element locus
            uce-27, sequence.
ACCESSION   KCVB01000750
VERSION     KCVB01000750.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35561pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1490
                     /note="ultra-conserved element locus uce-27"
BASE COUNT          443 a          248 c          396 g          403 t
ORIGIN      
        1 gataaaatat tttcccatat tttgtgaggc tgtaacactt agttgttgtg catgtttttg
       61 tctgtaattt ccaaggcccc actctgtgtg aagtctcgca gtctgcattg tccttgcatg
      121 tgtgtgcata cgtgtagtag tgcttgttag tgagtgaaat tatatttgtg catgaatggg
      181 tatactattg gttctgcaga accagacaaa gagacggaga gcagctcgca gaatttgcat
      241 tggttggagg tctcatcata aggataatgg aaatcaaata acaacctatg gtatacagtc
      301 agtgctgtgc cctcgcgcca cgcagcttac aggtgtgtgt ctgtgtatgt gtgtgtgtac
      361 gcgcgtgtgt cagcatttga atcgcgagtg aaataacatc tttctgatag agtgataatg
      421 aaaataataa catccctgac aaaatattca taaaggtaat gatgaatgcc atatttctca
      481 gagagataca tcataggcgt cttggttaaa agaaaacagt ggccggcgtc tcccacaccc
      541 tcaaattatc tgccgctttc acatttcact gatagcacag atgagagctg aggaaaatgg
      601 acacacaatt atgctcactt gtcgtatatt aatactgctt cttgacatct tccatggacc
      661 ctgagctgtc atctctttgt ctgctttaat tgaaaattaa tggttagtgc gttgccaagg
      721 caactacagc agtggtggtg ggtagcgagc cacattcaga cagacaccag tggcattgtt
      781 tgtcctgtct gaccttggaa gtcagaaaaa agaaagaaga aaggcagaca aaaaaaaaga
      841 cattaaaaag agaatagaga ggtaagggtt gaatgttgtg cattggagcg agggagaaaa
      901 ataaaataaa gttattatgg ctatgttttg gccaggtaga gagaaagaaa actgtggtag
      961 gggtgactga ggagggggac tgaagatatg atgtcaaaat gccaaatggg gagaattatg
     1021 tttgagtaag aaagcagaag aaggcctggt gattcttctc tggagtgacg ggattacctt
     1081 tgtgctgatg gagtcagagg gcaccttatg gccccataat catctggaca ctaggtgcgg
     1141 gagtgagcga gtgtgaaagg gagaaattgt atgtgtgtgt gcagaggaac gcgtggacat
     1201 gttggtccct aatgtttaca actcaatcag tgtggacacg gtgcagaatg gacgctgact
     1261 tcagggccag gcatctgtag attgacagag aagctgatgg acatcacaca atctcctcca
     1321 gtggcgagct ctgagggcca agatgaggag aggcacaaga ggttacagtg gacatgatga
     1381 gaaatctttt taaaaaatat aaaaatcatg tacagacgtg atttactttc agaagatgtc
     1441 agcaggtttg gtgcttttca tcattagtgg actaggatga ggagttatgg
//
LOCUS       KCVB01000751            1553 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35631pri ultra-conserved element locus
            uce-367, sequence.
ACCESSION   KCVB01000751
VERSION     KCVB01000751.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35631pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1553
                     /note="ultra-conserved element locus uce-367"
BASE COUNT          464 a          315 c          333 g          441 t
ORIGIN      
        1 tggattgctc agctgttggt tccggtttaa gtgttggtgt cggcactcta ggtgtggagc
       61 tgtgagtgat gttctcgact atcgtcgcca agggaacagc atccgttaca ggaggcaggc
      121 agctgacctt gttgatgcca tctacacatg aaaatgcttc tggacatgga ttcaacaggc
      181 agtcatcata gttgtgctca cagtttctcc cctgtgtgtg tcgaataaac acattttaat
      241 tgtgtggtgt aattcttgtg tgagatgcta aatgggactt gatgtttaca gcttgagtaa
      301 taaactcgga gtaagtagga aacatgacat tatgcaacaa aaaagatttt tttttaaatg
      361 tgatgcaaga ttatctgatt tgatctgatt agacattaca atttaaaata tggaatattc
      421 tttcccttaa taaccttttg tggtgcactt gtttatataa tgtagcaaca aatataaaaa
      481 tgcacatgtt gtcctgctgt cttatcaatg aaaggcatct catttacctg ttttacctgt
      541 tcttaacaga cgataaacaa caaagaaaaa ggcaatgact catttttaac aggtcaataa
      601 ttagcgccaa gcaggactca ggagttgagg gtgagggagg gtaaaggttc aaataaaaaa
      661 aagacacaca aaaaaatgat ggagttgggg aataaggtgg ggattctcac cacaaatcca
      721 ggacgacaga aacaggtgta gccatagcct ggatcattct gaatgcatat cccctggttg
      781 cagggctgtg gaaggcactc attaatgtca tcctcacagt gaaggcctgt ccaacctgcc
      841 aggaacaaaa gcccacagag tgccgtggtg agcccgggct ctcccacttc caccctccga
      901 agtgaatatc aatcagattc tcctacattg atttgcttct ttttgggttc acacacatac
      961 aaagacaaga aaaggctttc actaaagagt aagatgccac atacatttta atgagcccag
     1021 gctgtatcgt ctttttatca ctgtcttctt cagtactgta tgactacaaa tttctccctg
     1081 gagaagagtg gatgactttt gcatggcttt caggcacaga agttatatag gcattatggt
     1141 aacctttaat gacacttcat atggccccct ttcaatccaa tatgggcttt ctataagtaa
     1201 taacatggtt ttacatatga taaactgtac tctcccacat ctggggtgca gtaaaacaac
     1261 catctaggcc gtgtctcagt aacctcattc atgagccaca gaggtctttc ggtaacagga
     1321 cttataaatg aaaataaact gcattggttt cctcatttca aagtaaattg agagcctcac
     1381 tggaggtctg agtgactcac ctgacctgca gtggcagacg tagctattaa taagatcttg
     1441 gcaaatggca ccgttgtggc agggcgctga acaacattcg ttaacatcca cttcacagaa
     1501 atcccccatg aaacctggtg gacagctgag gaaacatatc atacgttata act
//
LOCUS       KCVB01000752            1559 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35677pri ultra-conserved element locus
            uce-1310, sequence.
ACCESSION   KCVB01000752
VERSION     KCVB01000752.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35677pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1559
                     /note="ultra-conserved element locus uce-1310"
BASE COUNT          480 a          344 c          303 g          432 t
ORIGIN      
        1 atcactcttt tattcttatc aggacacagt taaaggttaa aggcaccatc cctggattca
       61 aacctgcagg ctttgtgccc ttgagcagga cacagaatca gttcaagctg cagaaatgct
      121 gctctgtggc cggtctacag ggagatataa agttttatta agcagccagt acacaattaa
      181 ctgtaattag catgcaaaat cgatctacag tttatgaatt ggtgactgta acttaattgg
      241 tttgaaactc ctataggaca aatgttccac acagataata aaccttgcaa taatcagttt
      301 ataacatcaa aagtttggct actgtgtgac caacctcaga gacacagagt ttattgtcct
      361 atatgagctc agatttatta tgtgacttat aacaggatgc atcacatgca cttattatgc
      421 cccagagcat ttattgcaag aactcttaca aaaatgcata tggtcagtca cttactgtat
      481 atacagaata tactttaaat tcaccgacta cgtcaaaatg ttaaatcacc taaaaatatt
      541 tgagtaagac aatcttttaa aaagtatgac acatatgtga ctgtctactt aactttgcag
      601 aattccttca ctgacacctt cagcttaaat gaggcacagg aaaccttgca aaacacctat
      661 ttttgcaaaa tgtcattatg tggagggttt actttgacag aggcccttgg agttcttcat
      721 cttcttcttc ttgccaactg tgtccagacc ccgggacaca caaaggcggt acaagcaatc
      781 acacatcatg gactctcata ataagctgac gctgggaagt gggtgatgca gcactcagct
      841 tatgccagtg ctgataagag cctgaaagta ttacctcggt ctggcagccc gctccaagaa
      901 atccatcctg agccaggtca tcaattctgc cctatctgat tgctcaccaa tccctggcag
      961 ctccatgtcg ctgtcctggc tcctgccttt gaaattctct ctatgacttg cactttcatg
     1021 ctagttcttt cacagaataa tagacacact gtatgtacat atgggatctt ttatgtggtg
     1081 ttacagctgc ctgacaaaaa cataaaacag tagacagtgt gcctgggaag gtaaaatttc
     1141 gagagcaata gagcgcctgc cttagtcaat cccttaaatg tattcattgc aatcacatct
     1201 tccccctcat tgctgcagtg gacctataga gcatacatta aagcctgtgg agttcaatca
     1261 tatcacctac gctgtgtggc tgcacagtca atcaaacaga tgaacaatta attgaaaaat
     1321 attcaaccag agctgtgctg tgctccagtg aggcaggcag gcaggcaaat aagccatttg
     1381 attagctgat aagcagatga ctcaacaaac atccaaggca tgtttactgt ggtttgatta
     1441 gagtgcgccc aatacgcaga agattggaca caaatacgtt gtgaaaacac tcagctgttc
     1501 caatccccaa ggcagcatga gggcacgtaa aaacaatgaa ttatttatgg gggagacta
//
LOCUS       KCVB01000753            1589 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35722pri ultra-conserved element locus
            uce-432, sequence.
ACCESSION   KCVB01000753
VERSION     KCVB01000753.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35722pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1589
                     /note="ultra-conserved element locus uce-432"
BASE COUNT          449 a          308 c          358 g          474 t
ORIGIN      
        1 ttgttatcag cgcacagttc aaaagccagc atttgtgatg gtataggggt gcatcagtgc
       61 tcatggcttg ggtaacctgc acatttggaa aggcaccgga aactggggct cgctctctga
      121 tggaacacgt gtatcagcta tctagcctgt gctgaagtca gcagcgagaa ggtaagctaa
      181 catgtaattt agagaccaaa gcagaccagg cttctgcacc agcagcgacc aagagggctg
      241 tgatataggc gttagcatgt gaacttggct ccattagcac aaacttctta gcccagaaca
      301 aagcagaaag aaaatttctg ccacatggac acagaccttg tcgttttcac tgttattcgg
      361 gctgtcatgc aattatttct gtggggaatt aagtttgtgc tttgtctgtc atgtgtacaa
      421 tgttgaaaac tctgtcaact gcctataggt ttctgggctt ttacctcatg caagatgaga
      481 gatgcaagtg ttaacttggg gtagtttaaa gtcttcagac tataaaggac gggataacca
      541 tgatgactta tttttaaccc gatgacgtgt cctgtctgtt taatttgtgc taaataatat
      601 aaaaccctat cttcaagatg ttaaatgatc aaaacatttt ttcttttttt aaatgattat
      661 tttaacaagt ctgagactat gcactctact gaacacatcc caagtcttgc ccttgaccca
      721 ggattaattt ggctcattgc atttacacag atttctggag acatggccac agaacatatt
      781 aatggaaatg gtccagaaga accaatggac acctctgctg cagttaccca ttctgagcac
      841 ttccagactt tattagaagc tggtttacca cagaaagttg ctgaaaaact agatgaaatt
      901 tacatagcag gtaaggcaca atgaatatac ttgcatattc aaatatgcaa ttttttctgt
      961 ttatcaattt tttgcatgta ttgtcatttt attacctttt tgaatggggg aattgggatc
     1021 ctgaagcaac tttttgtggt ttcctaccaa aatgggtact gcagtgctac aaaatattaa
     1081 attacacatt accagtgtga aaatgtgtaa aaaattgcag catttgcatt cccaacatgg
     1141 ttgcatgatc actggtgtca gggcgaatga atagttcttc ccctgggagt gtcttggtga
     1201 gcctgtccag tctctccatt catttctaag aagtttctgt atcccataaa atcattgcag
     1261 aggtatgagt aggtgttaag tgtgatgtca gctacagctc aagtatctgt tgcattggtc
     1321 agatctttac aattttgtac agggtgaaaa gcagtgacag taaaagtttg ggggtcatct
     1381 taggacttgt ttggtcggtc acatctagaa aagtaccagt ctggcctttt cagccagatg
     1441 ggactgaggc agaaacaatg gcagcagctg tttggtgtat ggctgcctgg gacaggtgat
     1501 ggcccctcca tagagcagaa tgcagaccga ctcattagaa actgccagcc acagtataga
     1561 ggggcacagg catatcgagt tgcactgtc
//
LOCUS       KCVB01000754            1701 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35766pri ultra-conserved element locus
            uce-818, sequence.
ACCESSION   KCVB01000754
VERSION     KCVB01000754.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35766pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1701
                     /note="ultra-conserved element locus uce-818"
BASE COUNT          502 a          351 c          342 g          506 t
ORIGIN      
        1 gctatttcag ttaaaaccac aagccctgtt tgaggaagaa aatagcagac agccaagccc
       61 ggcacaccat gcaacaagca acagagaaaa aacagatgac gttgaaaaat ttcctttcga
      121 gcctgtttaa tatgtgttaa ggcgtagctt gtgagacctc tgagctccag taatttcagc
      181 acatcaaaga catgcctcct ccccaagctg gtgtagtctt aagaagggct catcatatcc
      241 aatcacacgt tcacccgagg atcttgtagg aatacctcca accctgtaat tttgttcatt
      301 aaaaccagac taatacaggc tggtcaacac cacaactaag cgctgtgctg tcacggcaat
      361 tgcagctggt agtccttgtc ccccgttgac ctgttttgtt aactctactg ctgtatttca
      421 ctgcttgatt agatgtcact caattaaaag cacatttctg cggggagagc ggcctcccta
      481 gttgccatgg atgctgtgag aaaggtagca gagcggcgta atgtgtgtcc cagagcacca
      541 taatgtaata tccacactgt tacagcagaa atacacagac aacccaaact ttcacaccca
      601 atcaatgaga gctgtgtggc tgcctttttt tacattgaaa aattaaaaag gatgtgccta
      661 gtaagtcaca gaggactcac gcattttata gtcagctatg agaggcacta ggattaatag
      721 acctctccta atatgcctcg cattggtggt ccctgaacac cctgatcagg taaggcccag
      781 tgaagcaaag atcatttata agtggttcat tattttacct tcagattcct ctgtctggca
      841 ttgatttaat aaacactgat gtatctcctt gtagaagaga agtgccattt ttcatttccc
      901 agatttgcta tctttgttat cgcagttatc tgtcgagctc tggcatttct gcggtaatat
      961 gctcccaatc tcagctgtgt acaggaagca acatgggaaa gtagtgcaat tcttctatgg
     1021 cagatattcc ttttaatgtt aactgaaaga ttctttaaaa gaaaatattg tcttttatct
     1081 ctctgactcg ttgcctgctc gtccagacat gactttgtaa atgaacagct tgtttaataa
     1141 agttctgccg aagtgctctg attatccatg aaaggattca acaacgtgtg acatcaacat
     1201 tgaggtacaa taattaatga tctatcagtg tgttctgatg attaatgtat ttgtttttgc
     1261 agtttgatgg acccataaac tgtgtgaaat agacaatgag ccattgtgta ttccctgtgg
     1321 tgaatagaag caacagcact gaggtcctct ataaaaaagc gtagaaaagt gacgaaaaga
     1381 aaggagaaaa acaggctctt tattattaag gttaactgtc agtaaaggct catgctcctg
     1441 cccgatcatt tttgtaactg agaagcttgt tgtatttttc tgctcaaagg attctccttt
     1501 gaagtgttaa aagcagtttg tggtcagatt aggtgtgtat ggctggcaca tcaaatcaag
     1561 atgaaaatga aatggaatga tcaatttgcc ctgcattaat ccctcctctc cagtggcatt
     1621 ctatcgattt tcttgttcag cttccttgat aacaagaatc atttaaaggt cacattttga
     1681 tgtctgtcaa aaagaaaaca t
//
LOCUS       KCVB01000755            1644 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35804pri ultra-conserved element locus
            uce-940, sequence.
ACCESSION   KCVB01000755
VERSION     KCVB01000755.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35804pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1644
                     /note="ultra-conserved element locus uce-940"
BASE COUNT          392 a          332 c          419 g          501 t
ORIGIN      
        1 tctactctat aataagactc tagttggccc ctgatgcctg gttaagatag gaagggagat
       61 gtgttaatta gactcattta cagcactagc tagtaaagag caggacccct tcacagacga
      121 cctgtttctc cttgagaaaa gccacagagt tacagctcaa agtttcacat ggaaaaacca
      181 ccggggatct ggaggtgagc tgaggtagta ccagtggact tctttaagac aagagcggcg
      241 ttggtagatg taatgggaat aggaattatt gactggagtg gcgtggtatg gaaatagtgt
      301 ctctggtgtg ccatcatcga gacggaaagc ttatagtacc ctgatttatg actgctgtgg
      361 agaattgagt gcttcataaa gatttgctgc tggctgagat ggagtaatca gtgaagtgat
      421 ctgactttgg tggattacag ttccaacgag ttgtaaagtc agttacaatg ccctgacttt
      481 gtctgtcgga caacgcgcgt ggaaggagtt agagtctaac tctggccctc tgtcaaggtc
      541 atatggatta gaaatgattg ggtttccaat actctactgg ggagttctgc atgatgaatc
      601 atcacttcat tccaaatcga caccaattaa gaagggtttg tttattccgc tgtgggacat
      661 gctgtggatg ttcacttgga acaagcattt tcgccccctt tctcttttca caagcaacac
      721 tgtggcattt gcagggatta gttaaatgtg tgtgtgttag ttgtgtgttt gtgcatttgt
      781 gtctgtgtgt gcttgcttgt gcacagtgga gttcgtattt gtttcaggcc tgcggatcca
      841 cattttattt gcacacacaa cacacagttt gtgtgtgttg tgtgtgcaca tgttggcacg
      901 tgcatgtcca cagtcatgtc actcagtgtt tactgtgtag ccctgctctg tttgcgccag
      961 tagcttcatg tctgtaaggc atgctatatg aaaagtgagg ttgtggagcc cagtgctgga
     1021 gccagtgaat tcatttgcat tcatttcact gcctgtctct cctcagagcc cattctaatt
     1081 accagcagct caggtctgct acaacactgc gtatactctc tccctctctc tctcagtgaa
     1141 aaaatgggct gctggtggtg cattcttgta gctttgatac tactctctct ttagcctgcc
     1201 aggaatctcg tcatgttggt ttgtttgctg tcaagcccag gcaatttctc ctgttttagt
     1261 gtcggagtca gaggaaatat agtgtatgca tgtgtgtgta agcgaaactc ctccatactc
     1321 tgtgtgtgct tgttgttatt gttttgggat ggagggagat gcagtgtgtg ttcatatata
     1381 ggagggagag tgagggggaa ggagggagag aatcatgagg cttgtaagag atgaatgtgc
     1441 gcactaatca ccgtaatggc cattgtggcg tttcacctcc tgagtctcaa acaccttgtc
     1501 acgccctctt cctggtgatg caaatttctg caaaggctaa taactcacat gacatcacgc
     1561 cactactgag catgctctag agcctcatgt gcatacttaa gcatgcatgt atgcacacac
     1621 atgaatgtat ggcatgcacc attg
//
LOCUS       KCVB01000756            1093 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35841pri ultra-conserved element locus
            uce-597, sequence.
ACCESSION   KCVB01000756
VERSION     KCVB01000756.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35841pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1093
                     /note="ultra-conserved element locus uce-597"
BASE COUNT          285 a          244 c          224 g          340 t
ORIGIN      
        1 ctcagtattc taatctcaaa ttcctatcat gacaaaccca gttcatgtag ctgtattatt
       61 atcaaaagat gcaaatacct cttattatta ttttattaat tattatgcat ttctctgtct
      121 gcataagcaa gatattgtga gttaaatttg actggattca tcattctttc agactgtttg
      181 gattctctag gcatacgtaa ataatcaccg ataaactata ggtcaatact ctgtcaggag
      241 gtttcataac atatggaggt attaaatcag tctaaaccaa atatcactga atgtctgagt
      301 tattctctgc atgaaaacag cttttaagct tctaaatacc ttttatccct ggtttggatg
      361 ctttactatc aaaacaataa cctagtaaaa caaccatcat cattatttgt tatcaatagg
      421 gtatctttgt tatttattgg ccattgacag caggaaacac caaaggcaac aacctgggtg
      481 tgtcgaggtc ttgtttagct tctattcaaa agtaatcaca aaatgcgcat cagtttgcaa
      541 gcagcaatcg tccgccctct ccttcccctg gctgtttgca ggtttgcatc tgaatcaggg
      601 gtatgacggg ggcccgagcg ctcggccacg tggaccagat tgatgtcatt gtctcggcac
      661 actgaccttt gctgtaatac ccacggcaga catcacaaac tctcacatca atttagtcca
      721 attggatgtg gcatgtcttg gctaactcaa tttgtggaga aatgcacctg tctctgcctt
      781 ccctccctat agatttactc aaggggaggg gggaggagga ggtggtattg caaggaagag
      841 aaagggagag cgaggggagg gttttggagt ggagttggct gctgatccaa taccctcccc
      901 cctcctcacc cccagaaaac actcacccac atgcatataa acaccaactt ctccctcttt
      961 ctctttctct cagctctttt ccctcgacca tactttgcag atgtagctct tctgcagcag
     1021 cacacaccct gaacacttac tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     1081 tgtgtgtgtg tgt
//
LOCUS       KCVB01000757            1426 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35880pri ultra-conserved element locus
            uce-905, sequence.
ACCESSION   KCVB01000757
VERSION     KCVB01000757.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35880pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1426
                     /note="ultra-conserved element locus uce-905"
BASE COUNT          411 a          298 c          314 g          403 t
ORIGIN      
        1 ttctcagtgg agtaagaagc taccattaaa ggtcaaagcc tgggaactaa atcggacatt
       61 tttcacttaa tttaaacagt atcggctttg ttccagtcga tgcagctgaa atagcacttt
      121 ggatttagat cgagtgaaaa ccatctgttc agtgagccag tgctcgtttc agttttcctc
      181 catattttct aaaaaataaa ccctccaata ggaaacacgg gctttttgat gatgacattt
      241 ttatgaagtg ctttgagttg aaccctaaat tttctttctc tagtcgtctc ccacaggact
      301 ggacagtcac agttacagtt catgtgtttt ccccaagcac ggtgtaacaa tttctccttg
      361 agttggcaca tagtttggtc cattgcttcc atcaatactt aatatgtctt cttttttctc
      421 tcttaattgc tgtcatgacg tgaaatagcc taattcaata aacaacagcc aacacacccc
      481 caatttctca tagtctagtc tacattaaac caatttaatg aaaaaagggc aaaagagttt
      541 attttctcgc ggtgaaaaac atcctgactc gtttggattt tgcggaaaag ccatgaataa
      601 aaacttggac cctcttgata tttagcagca tgagctccca atagaggcag gcggatttat
      661 tgcggttttt agggttaaca gaggagttaa cggagagaaa acgcctgtaa cgagagtaag
      721 gaccctctgc tcccctgtaa ggcccatttt gaaaggagta acgagttgta gcctacctaa
      781 gtattgcatt acttgtcgtg ggtacgagga gcccagctct ggccgtggca gaggcggagg
      841 cggagagttc acatcttgaa ataatctcgc cccctgggct gcacgggtta aaggaggagg
      901 cgcggcgcaa tgtggtagag atattagaga aacccgagag ccgctcatca gacagctcat
      961 ttactgaagc actgaggttt tacacacagc cagagtgcag ctctggagcc cgagctgaag
     1021 tggcctgaga gcctgtgtgt cactgaagat acggttcaac atttattgta gatgtactgc
     1081 ctgaatcatg cttaataaga ccggctgtgt ctgaaagacc agtgctgtct gaaagcaaga
     1141 aaattcaaat gtttttaaaa aataaccctt atatttataa aataacgtgt cgttgcaaag
     1201 tccaagttga tgtttttgtt cattttcagg cctgtataaa atgatctgcg catattgttt
     1261 gcctgcaggc tggtgacact gctaaaaaaa atatggctaa aaaacagtgg aaatgtactc
     1321 caaccctcgc aggcaacagt gacatcaggt gtttcaggca ataatctgat atttgcagcc
     1381 tggtagccac acttttaatt tcagcacggc gggatacgag acatcc
//
LOCUS       KCVB01000758            1565 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35908pri ultra-conserved element locus
            uce-1288, sequence.
ACCESSION   KCVB01000758
VERSION     KCVB01000758.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35908pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1565
                     /note="ultra-conserved element locus uce-1288"
BASE COUNT          488 a          340 c          321 g          416 t
ORIGIN      
        1 taaatataat ctgcgatttt agttgtacat acacttttta tgttttgtca gagtaaaccg
       61 tgactcaaaa cagatgcctg ctgttgcatg atttaaagaa aaacaaaaat cattttagac
      121 tagggccctt tttaatgctt catattgggc caacatcaca tcataacagg cctatttccc
      181 gtccccctgg tgcgcacaag cttataacag acactaggaa cataagttaa tgacacagtg
      241 ctcagtgaaa agcccatttc atcagagtaa acattttaaa taaatccaaa aagcgctcag
      301 tggctgatga agacagtata tagatttatc aaatgcactc aacaccggag gaaattaact
      361 taccctgccc cgctgctgtc tacattcacc tcgaaagaca tttgctccag gtccagctta
      421 tcttgtcatt tcaccctcaa ttattagaag tattgacaaa atgttttctc ccttctctcc
      481 gtcctaatgc aggccgttaa atgccaagct ccacgggatc gatgttgaac aaagcagcca
      541 gctgcagtgg cgttcaatat gaaggagata tttgggacaa tttgtgggtt tttatccaag
      601 tgaggctttt tttccattct cataaatgca gggataatta gggtaatttt tgatgtagcc
      661 cgctgattac agcgttttta ccgtcaaaga taattacctg taattttcta ccacttttaa
      721 tactaaaagg catctttatt tgggtttgag gtagcacaga tggaacaaaa gggaaaatta
      781 ttcggttatt catatacaaa ttgctgagaa gagtgagcgc tcgggatatt atctgaaatt
      841 acaacgctga aaagcttcat gcagctgtca agtcgcggat gtcccactgg cgcacattca
      901 gtctacctgg gccccacaga ctgggggcct atctgctcct gggccatgtg caccctgatc
      961 ttgcaggctg cagggagccc gaagcagcag tcgagtaaac atcaacagag ctaataaata
     1021 cgcagttgtg ttattcagct ccaagaactg acacgtttca tcatgttttg aataaggaaa
     1081 aaaaagactt tgatgaggaa aattagctga gctgaagata gatagataga tagatagatg
     1141 gatagatgga tagatgaatg taaagtgcag cagagtgaca gatgtggggg tggggggatg
     1201 cagggaggga cgtgtcactt cttgctctgt gagctctggc agagcagtag ctgatgcact
     1261 atacagcacg cttctcaaat aaaagctccc agcacctgac aagatttcac acatcgccac
     1321 atcagctcta tacctcacca gcgcctgagc aaacctctca caaagaccca caaatccaaa
     1381 tattctccac aacaaataaa aacgaaacca gatttaagga aaacgcatta ttttcttcaa
     1441 tctctctgcc acagtcgcga ctaaatgatt aagctcagag cttcctgaag actaattttc
     1501 agtcaagagt gttacagcct cctctaagat caggaatagc cacaggacag aatatgcaag
     1561 actca
//
LOCUS       KCVB01000759            1851 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35952pri ultra-conserved element locus
            uce-1174, sequence.
ACCESSION   KCVB01000759
VERSION     KCVB01000759.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35952pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1851
                     /note="ultra-conserved element locus uce-1174"
BASE COUNT          486 a          433 c          436 g          496 t
ORIGIN      
        1 gttccttcct ctggcacacc agctgcgtga agctctgctg ttgttgctga ggcagacgct
       61 gcttacgctt gttctctcgg ctgctactgc tgcacacctc acctccccgt gggctgctgg
      121 gggcctgctg ctgtgactga gggggtgggc agtctagctc catctccctg tctccatcca
      181 tctcatgatc tcgctcatgg cgggaagcag caggtaccac cacactgggt gaatggctca
      241 taccacgtat gatattctct acacgggctc gcttggcacg caggtgttca tcatttagac
      301 gttcgatgtc aaaggtggcc aggccaggag gacgaccaaa tggtgacaag cactcttgag
      361 gggtgctgcc ctgggaggag ttactgcaag catcctcttg tggagcatct gaacttgcat
      421 tggagagccc tgagccgggg aagccgacat cccctctatc acccctgtcc cctctttctc
      481 ccctctcagg tcctcctcca tttttagcca tgttattctt gagaagctgg gagatgatgg
      541 tggtgccagg gaagggcatc atggtatcct cgtatgagtt ggccctcttc agtagctttc
      601 ggagtacatt ggacttggac tcactgtcgc tggctgatac aacgccaggc ccaccatgtg
      661 gctgcactac agagcactcc atggagtcgg cgtctgatcc gtggtgagag tgggagctca
      721 gagagttcat ggcactgaaa atggctgctt tggcgcgggc aatgttatca gtggttgctg
      781 ttgtggctgc tgctgtggag gagactgtgc tgcccacggt cctcttaaca ccaatgtcca
      841 cacgtcttcg cttggtctgt ctgttcagga gggagtcgct gtcatggtcc ggcatcacgg
      901 gctactgcta ttgggccata tcccacaaac ccagtcctct aggattaaag tctctgagta
      961 cctgcggaca aaaatttaca gatgcataac tcaccaaagt gaaaaaaata tttcagattc
     1021 taagtcactc actcactcac ccagacaaaa tgtgtcctag aacacttcag tcacattgaa
     1081 gaaaaaagct tgtaagcatt tgatagactg aacaatcaaa atcaatcaac ctctagataa
     1141 ttgcactcgc caacattacg gatataaact gaagagtttt gtctcaggca agaaggcaac
     1201 ccctccaaat atgtttctac aaggacaggg acatccgtgg ttagatttag gcagtaagga
     1261 tcagtgacag aggggtaaga gaacaggcaa gacggggtca gaattaggcc ttggctcgac
     1321 attccagggc aaagtcctac attttatcct gtcccctgat acacgccgcg cgacactgaa
     1381 ccagttccca cggaacacaa agctgcataa aattatgata attacttaag tacttgtcct
     1441 catttccatt ttgctgtggt gtgagccagc ataggagcta ttaattcata gaataaaata
     1501 aaaagaaata gggttaagag taaaaaaatg tggtgtacaa agggtaggca agcacattat
     1561 atatatcttt ataaagactt cactaaatac tgtagtaatt aagtgagtat tatcatttga
     1621 tcaattcaac aaaatattct cattttaaat catgtttata cattgtgtaa ttttaaaaag
     1681 tcctgtgtcc acattaatca atatggtcat catatcttaa gcaatggaga gagtgacaca
     1741 taaaagaaac acaaaatagg cctattgatt ttggtgccag catgagctca cttctgtgtt
     1801 ttttgtttgt cttttcatct ggcaagggca gttaattctg ctcaaatgaa t
//
LOCUS       KCVB01000760            1468 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 35979pri ultra-conserved element locus
            uce-1131, sequence.
ACCESSION   KCVB01000760
VERSION     KCVB01000760.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="35979pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1468
                     /note="ultra-conserved element locus uce-1131"
BASE COUNT          407 a          301 c          278 g          482 t
ORIGIN      
        1 gtgtgtgttc gtgtgtgtgt gtgtcgtctt attccagtct ctgccagagt ctctgccact
       61 agcctgtaat tatgatccta gaggtaattt tatcaggctc attagctctg atgagtaaac
      121 gagaagcgtg cctctaattt attggccatc caggctggag acttaattct catcagagag
      181 atcataatat ttgtatagag agatagatat acagcaaata tgctcagtta caaagctcct
      241 tgaccaacac tgaggccgag acttttgtga cttaaaactt gtaattacgc tttaaaaatc
      301 aagtcagttt ttgcataatt tggcatgcgc taaaattatg tgtttagata tcagtctgat
      361 gagcagggca acagaacgtg gactcttttt gattcatgca tgatttcaaa tgaaagctat
      421 tgtttgtatt ctacagagtc ctgtgacttt accctcttgc acaaaattag atgctcaaac
      481 ctctgcaaac aaaattccat tgttttgttc cctgttcaat agagatgatc catcttctgt
      541 gtcagagaat gtgttaaggg agcggggtaa atatgctcac aattttcagt gtgtagaaat
      601 ttgcattgag gggaaattat gcatgctggc tagtggaaat gaaatgtata aagtgcatga
      661 ctggtagact tgacaagtgc agctttcatc catctcaaat gaataatttt cacttccctt
      721 aaacatcctt aatccaacca tgttatcagc gggcatgcag gattctgcca gactttctcc
      781 ttaacggcac agcaactttc aacttgcctg agattctgct ctgcagttgc ttaccacatg
      841 gtcttaggga ctcactctta tcccttcctg ctggttttgt ggatcagtaa aattccttcc
      901 aaacacaata tatacataac gcaagtctta atagatagca gaatgtctca ctctaccctc
      961 ctctgtgctg cctcagaggt cgacttaatg cactgtattt tacctctgat tcacattggc
     1021 agagaactca gaggatggtg tctgaaaaac ctatttgctc agtggccctt tgtcctctat
     1081 ttcaaatttc agtgacagac ccttccatgc tagtataagg tggtttggaa attcatatgg
     1141 ccgtaaaggg atagagaagg atattcatgg taaattactc atttacaccc cagcattcag
     1201 cactttgact tatttgggga acatttcctt gtcttcttca ctgaaataca tatgatgacg
     1261 ctacattgtg gcatttaccc ccctctacat cctaatgctt ttaatttatg ctctgtagca
     1321 tttttcctaa atactactga caataaacaa ttaaatgtgt aatttgtgtt tatgctgcta
     1381 atccttgtga tagcctctaa tatgaagggt tacctggcta ttttcaatat ccactttcac
     1441 agaagcctca gttagcgata agaatatt
//
LOCUS       KCVB01000761            1445 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36115pri ultra-conserved element locus
            uce-1189, sequence.
ACCESSION   KCVB01000761
VERSION     KCVB01000761.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36115pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1445
                     /note="ultra-conserved element locus uce-1189"
BASE COUNT          462 a          274 c          252 g          457 t
ORIGIN      
        1 gctgacacac atattttatg atagaaaatc aactctaatg ctgttatttc ccaatcaaga
       61 aatacatatt tttgtgctct ttatgccaaa ggaataatca atatatgctg tatttctctt
      121 gcactatttc tccaccaaca ctatctgcct ttatataacc taatcccacg tatttcttta
      181 tctctgtcaa caggattaac tttaaaaaat gacttttata aaagacaaac ttgaaaatgt
      241 cagtcagcat atcagatatt caggaaatca gacatccagc aatgggacca aagtgacatc
      301 ttttcttatt cagtcattga aagcacaaag taaatattag ttaaaaggag cagatattat
      361 gaatcttctg ttgacacctt atgatgattg ataacacaaa aataagagaa gttgtggctc
      421 aagtgctctc cacacgagat cctccgtatt actcgagatt cagcatgatg agcttcaggt
      481 aatgagattt ctcttttttt cctccataaa aatgcatata taaggttctg gaaacaaact
      541 cctcaaacgc agacatcaaa acaaacggtg aattaaggca cggcgctcgc tgccttgttc
      601 tgagagcatg gccttgagct accgattttt aaactgtgcc accgttaatt tatgcgaaga
      661 tctgaactaa ttctttggca actgtgaagc gagaatatta acgcgatgta tttccttgtc
      721 gatgataatg ccgcgctctt aatttgagat gtgcagtgta gtcgaacatg aaatggaaat
      781 ttgccccaaa ctatgtattt gtcagtgagt gtcggatgtg gaatgaggtt acatgcatcc
      841 tttgctaccc acactcatac aagaagaggt gtgttccccc ctcaccgccg tgtgttatat
      901 tgagaaagct tcaacttaaa gccccgcttt agaatatatt atatttacat atttttatcc
      961 agggtggttt ttataaagaa tctgcataga tttcctgaag agtttctctt gtgcatggaa
     1021 aacaaacata aggcaggttt tcctccagcg tttccaacta acctctttga ggtcaaagct
     1081 tttttttttc atgtctttca tgtgtagaaa gcagacaatt attcaaaaat tattagttag
     1141 tacaaaatga gctttaagta tgaagtcggg ttaaacaaca ggctgcacag aggtcctgaa
     1201 gacaaaaaat atttgacgaa agtattaaaa atactcttaa atctacatcc tacagaatgc
     1261 aagtacaatt cattgtacac cttctcttaa acatctgaga tgaaaaatca tctctatact
     1321 ggaaattatt cataaagccc agagtgaagt gaaggactgt aagaggttgg tgctacttta
     1381 tgtttctgtg gctgaaccaa atattttctt tcagattctt acagtcagtt cattcagttc
     1441 tagag
//
LOCUS       KCVB01000762             878 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36155pri ultra-conserved element locus
            uce-1230, sequence.
ACCESSION   KCVB01000762
VERSION     KCVB01000762.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36155pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>878
                     /note="ultra-conserved element locus uce-1230"
BASE COUNT          263 a          223 c          134 g          258 t
ORIGIN      
        1 cccccccaac acccccctca tctcccccat cattcggaca gctggaaaca gaattcatcc
       61 gattcgttct gctgcttttc tctctcccaa ctgccccatt ccccccacca ttccccagtc
      121 cccctcactg tcgtaatagg atctgcagga atcattcaaa ggctagatat aatttactgc
      181 ttgctgtgca caatacctcc tcccactctc accccccttt ctcaatcgca caaggtgccc
      241 tgcggatgaa ctaatcatac ttgaaatatt atttctgctt cttattcctc cattttatga
      301 acagctcctt tttatggtgc aatggattta acccctgcct ggtcacactc acaccagtct
      361 gtgcaggaag aaagcgggtg ggtgggtggt gtgggcggag agtgaaagag agaaaaacct
      421 tttgttttaa tatttctttg tttgtgtttc tgccttcttg tttgttttga taaatttgta
      481 ttttatccaa atgttgattt ccccaggtga agagtctatc tcacaaaaaa aaaaaacatt
      541 tcttagggaa catacaaata atacagccaa gagctttacc aacaaaacca taaaaactat
      601 tccacagggc tcttttaccc ataatcccct tgctctcttt atgcaatcca catatttgga
      661 caagtaactc ctcttcattc ataatatata ctccacagta aatgttctca tgtataacag
      721 ctagtattag catatggact gcgctgcaga aataaattta aaaaaagcaa agaaaacaac
      781 acagaaacat aaatttgaaa ctactaaaaa aactatgatg cacctcaaag tttctgttta
      841 aaatatgcca caggcgcaac atgcaagcat tgagtcta
//
LOCUS       KCVB01000763            1692 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36231pri ultra-conserved element locus
            uce-752, sequence.
ACCESSION   KCVB01000763
VERSION     KCVB01000763.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36231pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1692
                     /note="ultra-conserved element locus uce-752"
BASE COUNT          543 a          361 c          332 g          456 t
ORIGIN      
        1 tacctttaaa aacaacacca catgcataaa gttagaaacg acaaaaatgg ccaagataca
       61 aagaactact ggcctgtgtt gtttaacaga aggcagtgat ttgcctgata taagtccaag
      121 ggggtataaa tttgaacttg ataaagagag gagtccctct gatcccattt tcaaacacaa
      181 agcttcagct aactcatcta caagccactg caagggctct ttgtattgtt tgttttgttc
      241 agcattttga agttgttctc tgctgcaaag aacaccactg catctgtttc cggccataca
      301 aagcctgtct tactccggta tatcacactg tgatgcttta ggtgcataaa caaatggaca
      361 gtgatgttct ttcaagactt ctgaaccctg taacacaaac agaaaagagg tatccacata
      421 ggttgtggtc aaggacactg ttcgatcaaa tagagtaaga acagtgtaaa gaaacacata
      481 caccaatctt ttggtatgtg atatttgacc aggtgacagt ctgaaatttg gttttccagt
      541 tttcttactt acaacatttc ttttgtttgt aagttcttta ctagagccgc tccacttatg
      601 tttgctgtca acataaaaac caatggcaat cctcaccatc gcccctggtc tattggcaaa
      661 aggctgctgt catcatggct tccggttagg tcagccaagg atcaaggggg gcaaaggtca
      721 cacacacatt gtaaggtgaa agccaaggcc aaacgggaca ggcagtcatc ttagccagtc
      781 atctttagcc tcaatggact ctcagcctca gcccgcaact ggactctttc aaattgaaac
      841 atcaaggact tttgttaatg ttgaatataa aaatatatac cgttaaaaat acacaattca
      901 catgaaaatg tttgtctatg tctagtccta tagattaagc taaaactaca ctagctaggt
      961 ttgtggtgat catctgtgat tagctgtctt ctgtggtgca aacaacgaat ttgaaaattc
     1021 acaccttcaa aacttggctc accaaattaa cagtcatcca agagaacttt gcaatccatt
     1081 aaaagaaagt cctgctttcg agctaatgcc aagactacag cctagaatct taagctctgc
     1141 catatgaggg ctcgggcttg cagatggttg ttccttccag atgatgaacc acagatcaaa
     1201 gatcagccca taacagctcg agaggaacaa cgacctgcag gtcactaacc cttgatggtt
     1261 gcaactgaga ctgacaactg ttcagcagga tgaggtcgaa cacacacaaa aaatattaaa
     1321 ctggattccc tgacaagaag aatgaagatc ggtgctggtt aatgggaact cttccatgtg
     1381 gtcatgctat agaagtataa atagataagt gctatacaat agtataatac cgaaaattat
     1441 actgttatag caactaatga agtttgcatg caatctgagc cattgtacgt gtagcaacgt
     1501 aacacattaa catgggattc aaggctgatt atcataacgt taaaccgtga cagtatacct
     1561 gaacctccaa cttttaatac acatctatta gcgccggtat gaaacaacaa tacagctctc
     1621 tgtgcagtcc acatcgacac ctcaccccaa ggggaagggg aagagagggt atcttgtaat
     1681 agactgcact ta
//
LOCUS       KCVB01000764            1341 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36302pri ultra-conserved element locus
            uce-1008, sequence.
ACCESSION   KCVB01000764
VERSION     KCVB01000764.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36302pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1341
                     /note="ultra-conserved element locus uce-1008"
BASE COUNT          438 a          259 c          280 g          364 t
ORIGIN      
        1 aggcttatgg taactcagca ttatgggaat ataaaaattc cacctaattt gactcttaat
       61 cctcaaatgt taatgcagag agctgatttt tatctgccag tcctcaatga cgtgcaatca
      121 gacaataatt ttgaaatgag agtgaacgtt aaaaacaaaa agctgcattg ctgtaaacaa
      181 gtgaaaatat tataacgtgt taagatggat gtatctttgc actgtcatga tgaatcggtc
      241 tgtttaggct tcatttttaa gcgcctgtgc tctggtgcaa catttcagtg cagggacgcg
      301 tacagagaaa tggacggaga tagaaataaa aaggaagaag tgcaaaggag aagggggggg
      361 gggtgagcag gaggagggga agcaagcgga aaaagatggg caaaaaacac aaaataaaac
      421 aagcctttca aaccacacgc actcttctct ccccacagtt tgacactcca tcaaactctg
      481 atatgtcctc catttaaaga aacatgaatc tttctaattc catcattaat aaagataaca
      541 tatataaaag agtgtgtcag gctccatcag ggctttcatc tcttctataa tgaggttaat
      601 gcagagacgc agtgattacc ccagcaatgc aaatgaaggc caaatgataa cttgcactat
      661 ttaaataagg taatagcgat tactctacgt tattcaaatg aggacagatg ctttgaactg
      721 agcgctttga actcgctgct ccttctctaa aacatatgaa agacatggag gaggactcac
      781 tttggaatca ttcagtgtgt atcctctaaa tcaatgtgat gtaaaccaag ggcaacgagt
      841 aaagcaaagt cacttatagc actttcacac tgaacaagaa aacacaccaa caccttctaa
      901 cacctttttt ctttaacgag aatgggtaca atcagcaagt gactctgcag tggtcaacat
      961 gctagttttt gccccttttt gattacaccg ctatgacatg ctttgaattt aaacattatt
     1021 tcatgctttt tacaatctga taacaacatg caacggtgag tttgtaccgt attgtgcaag
     1081 atgcaacact ggcaagacag caaggtgaga gagcttctcc tgattcgagg agaagaggaa
     1141 ataaagagat agtttgctgg cgcgataaac aacaacatag tccatccatc tccgctgagg
     1201 cagtgctgga acatcgttca gtcggggtct gagtttagta aaagatgtag aaaagtaatc
     1261 gctgtaatat tttctctgat ctccgtgctg ctttctactg tttcctgttt ttacggtgca
     1321 gtcctgaaat cgaacctgac a
//
LOCUS       KCVB01000765            1321 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36360pri ultra-conserved element locus
            uce-453, sequence.
ACCESSION   KCVB01000765
VERSION     KCVB01000765.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36360pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1321
                     /note="ultra-conserved element locus uce-453"
BASE COUNT          369 a          279 c          321 g          352 t
ORIGIN      
        1 gtaggccagt ctttatgaaa tactagacta ataatgattc attgtttaat caagataggc
       61 ctgtgttttt catccgatat cacattcaac atataagctc tttctaataa attggagggg
      121 aatttggtaa cagctgcttg atagtctagt gagactgaag gttgattgaa acaaacaata
      181 tatatttttt ttgggtgcag agtaactatt ttaagttaca tctgaaaatc cctcacatga
      241 taaataatcc cacctgttct ctcacgttat aaaattacac attacataac ctttttttaa
      301 tccaatttta atctatttat tctaattttt gctaatgata catgtggcat ctttataacc
      361 aataaatcag aattctgctg tcaattatat gattttttct tacatgccta ttctaatgtc
      421 atctaatctg ctatcttgtg ttgtttctca ggaagaatcc tcaacaggtt ttcgaaggac
      481 atcggttacc tggactctct gctcccatgg acgtttgtgg actttatcca ggtgagtctc
      541 acctcggcag gtttggtcgc tggcgctgct gcatgggaag gccatttcat gcctggctaa
      601 acctgctttg tggtccgggt cccctaatct ggaaacaatt actgtaggat tacccgccag
      661 cgcagcccag cgcctgcaca gatttaataa aagtgtgcac ataggctgag ggcgtggctc
      721 cctcagtgtg acacaacaga agcggcatct tcagagaatg tgtatggaga ctaagcaagt
      781 gattggaaga gaaaaaggta ccaaagagga aataaaggcc acaggatgat gggtccgatg
      841 tggaggaaaa cagcggggag ggaggggggt gacaaggatg atgtgttgag tggcaaagtg
      901 aagaagtgtg agcagcaagc ataggatgtg tttgaaggag tggagctgca agcgtggtag
      961 agggggaggg ggacaggtca gttggaacag atccagagtg ggacttgccc ctctcagcgc
     1021 aattccccta atgctctaca gtcttacagg cctggtagcc aacggccacc ctgctgcata
     1081 accaggttag gttaccaaat gccaccctgc caccgtgaca gccatctccc acgaaagcct
     1141 gcggagatga gccagctcat gcacctagcg ccacaaagaa gggaatgttc gctgcacaat
     1201 tcgatgtaga ggagcaggcg tggctctttg gtttgggcca aagaatagaa caaaggcgca
     1261 ctgaaaacga atcccccctc cacaatgcct gagtgatgtt tgtggcagat acccatctca
     1321 c
//
LOCUS       KCVB01000766            1401 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36434pri ultra-conserved element locus
            uce-884, sequence.
ACCESSION   KCVB01000766
VERSION     KCVB01000766.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36434pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1401
                     /note="ultra-conserved element locus uce-884"
BASE COUNT          431 a          346 c          353 g          271 t
ORIGIN      
        1 acacacacac acacacacac agacagacac atcctcatcc tctttcggtg acagccagcc
       61 tgttaaggac atggacctcg ctctcatctt tcctttcact gggtctattt ctgtctctgt
      121 gtcattttat ctgaagaagt gtgtgtgagt gcagctcggg ggtaagatca tatcactatt
      181 aggggagtgt gttattagtg tcacaggcag ggtgacaggc caggccatac cacttcacag
      241 atgttctgct tggtgagcct ttcataagct atgacagaga gtagaaagac agagggggta
      301 cagtatgaga gttcttgaag tgtattccct cactaagaaa actcctgctt ctcagggcca
      361 gtggacaagt agtagagtca cacaatgtaa gtggtcctaa aaacctcaaa ttaggagatc
      421 tactgtacag acaggtgcta aaagagtctt cataaaccct tttagcaaaa acgacagaca
      481 tacgagagag acaggcagag accgactctg gtgtcccctt gatatgacag tatgctttta
      541 aataccagat aagaggaaac cacgagatag aggaagagga aaaatgagaa ataaataaat
      601 aaaccaaaaa aaagagaaca agatggactt gaaaggggat agaaaaagtc tgttgcagtg
      661 aggggggatg actgcagagg tcccagcggt cccctcaggg acctaggggc aagtctcagc
      721 cttgtcatac tccgctcttc tcctcctcca ctccaccaac cagctccagc cccctcccac
      781 cccccctccc ttccaccacc cctgttctgc ccacggaaaa cgtcgatagc aggctgccgg
      841 tgccgaggtc tctggtggac actagctcat tctcctggca tcgacaggcc cgccaaatca
      901 atatttagtt ccccactaac aagctgcctc ggggtctgag ccagagagag agagagaaag
      961 agagagagag agaaatgcag gacagtggag gatggcagag agagacagag aacagggact
     1021 gagagctgtc cagctagaag ggaaaaacat ggattcagta gagtgttgtg tgtgtgtgtt
     1081 agtgtgggag ggggagagga aggtggaaag gtcagaagaa gatctgggga acaagagaac
     1141 agtgtctggt gtgtgtcgtg gtgcggaggc gacgcatgag tgcctgcggc agccgctgac
     1201 aagaacacag acgagacaca catacacaca ctcgacactc tcaatcatac acatatcaca
     1261 gcgagcagac gttgacagca ctcgagtaaa gctccagcag agcactcaca gatgagtggc
     1321 tgtgcacaca aagactcaca ctcacactca cacacacaca cacacacaca cacacacaca
     1381 cacacacaca cacacacaca c
//
LOCUS       KCVB01000767            1744 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36467pri ultra-conserved element locus
            uce-1309, sequence.
ACCESSION   KCVB01000767
VERSION     KCVB01000767.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36467pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1744
                     /note="ultra-conserved element locus uce-1309"
BASE COUNT          454 a          348 c          317 g          625 t
ORIGIN      
        1 ggcacagttt gtgtgtggct tttttgttgc agtaatttgt aatctgtatg taaggttggc
       61 atctcttaag gggatgggga gggggggtgg ttagtgtgcg cgtgcatgtg tgagtgtgtg
      121 tacatgtgtg ctgtttaact ccattgtgca attttgaccc ctaatcaata aaaacagtta
      181 gctaatgaaa aaagtctgca tgcttgaggg catgcgtact cctttctgaa tcttaaagtg
      241 cactcacagt gaagcaatga ttatcgacag ggtgcatgca tccagtggat tgcatggctt
      301 cattgtttag ctggcttgaa aaagcagtaa ccactcacag atgaactacc actatgatgc
      361 cactcacatt tgtaactaac taactccgtt ttttttttac ttccaggtta gccgttacca
      421 ctaactgtga ctgcatgctg tttttttatg tctgttttat ccgtagcttt aatttcactg
      481 ttattttgca ccaacaagta atgaatatac tgattccatt cactattttt acactgtcag
      541 ttacagttag gactcatttt ggagtcattt ttttatccct gctgctcttg ctttaggctc
      601 ttctgcatgc atttgtgtat acagtgttga gttgtagtgt aatgtgacgt ggaagttgtc
      661 ttggcccagt gatctgactg ctaggtttca attgacagct gaggaagtgt gactggagcg
      721 tgactcaaaa cggcaggcat tatggcccca gtgccactga cgacactttg gggattactt
      781 ggcaaaggta accttttcat tttttcatgc catctctctg gcactcttac accacacaaa
      841 cacaaaataa cagcatataa taaatatttt cctgattttt tttttattcc ttacatatta
      901 aaaaaattgc atatgtcaca tataaagtca catatgaatc taatttatct ggactgtggc
      961 tctaagtgtt aatgctgaag tggttcactc tggcacagag gtttaggacc tgtaagatga
     1021 gatcatgcat tgctttgata aaagacattt ccttgtgtct ctcttatgtt ttttgttctc
     1081 cactttttct tcctctatct gttttcactc ttgcattatt ttccaccttc cccagtaatt
     1141 tgtagtgttt ttactttgtt agatttttta cattttttgc gctcacacct cataagagat
     1201 tagtgctgta ttttaggatt ggtctctatc ctgatttttg cagttttgcc taaaattcca
     1261 tttgtaatta tgttgaagag acttttttat tgcatcataa tataaacaag tactagtctt
     1321 tggaaagtaa attattatta tatctggtga aagcaacaaa ttattgcaac ccatttttta
     1381 aaatttgtca tgattatgtt tcactgctct gttatttttc actgtaagat gacaaatatt
     1441 gcacaacgat ttagggtttg aaacatggtt ttcctcttgg attcacaccc aacaaaaaca
     1501 aaatattctc atgtcaaaac agaaaccttg atgaagctca ttagcacaaa tgaagtcaaa
     1561 ccacatagaa taaaacaagt catttcaggt cagctttcca gccacgtgag gatgctttac
     1621 tctccacgtt ttaacaagcg tagctttgtt tgactttatg ctgaagtcac gctttactct
     1681 ctttaactta aagctacaga aatccccccc ctccctaacc cttcgccttc cccctccccc
     1741 tgcc
//
LOCUS       KCVB01000768            1684 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36510pri ultra-conserved element locus
            uce-1251, sequence.
ACCESSION   KCVB01000768
VERSION     KCVB01000768.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36510pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1684
                     /note="ultra-conserved element locus uce-1251"
BASE COUNT          455 a          379 c          347 g          503 t
ORIGIN      
        1 gacattttat cgtggaaatg ttaaatacta tacgttgaaa tgacagtacg ccaatttatc
       61 ttatcatgcc atgctactga tgccatcata aaatagggag cagaattccc ttgtctaata
      121 aataaagaaa ttgatgacaa aatgtatctt tggatttcac tgtgagaaat gaagcaagga
      181 tcacctacca tcaagatggt aattcattaa gaacttaatg gaaaatatgt cttagaagct
      241 gatgcagcct gtgggagaac agaaagtact cttttccttc atatcataac atttaaacac
      301 atcacttttc ttaagcagta acggtactac tactatcctg tcatgcctga ataaagccat
      361 aacaaacatt tatttaaaga cacctgcata tctaaatttc atgtcaaatg acatatttca
      421 tgtctgaaaa gaacttaaat ttatccagtg tactctcata tatgttgacc tgctattatg
      481 atagattagc aattgagtcc ctagctgcta ccattagcta caatgtcaag agagagcagc
      541 tttgacactt acctatagct gcttctatct tctcctcaga gggcttgtcg agaggctctt
      601 catcaatcca ttcaccacct acgctactta actccttttt ggtcccatgg atctgtattt
      661 tagttgattc caggtgtcca aagtttacag gcatctattt ttttttctcc tccagaacaa
      721 taaactctga ggggctcaag taaaggggac cgagtgggga agggggcgcc gaggtagagg
      781 ggctccgttt attagacagc aaatagccgg ccagtgggtt tatgatgtgg aaggtcattg
      841 ttggttaata agtggccaca tgagtgcagg caggtgctgt gttaacacct cctcttaact
      901 cgcctttcca gcagaggcca ttcatcagcc cagtgttaat gaaggccata taattaatag
      961 gtggatggca gagaaagccc cccgagttaa tcaatgcagg cctgtttgaa gagcagggct
     1021 aagatgggac acagggggtt cctaaatggg gggttggaga gtgtgggctt tttaggagac
     1081 acacaggtca ctgagacaga gagtgggaac tctgatggcc ttcactccca agcttcactc
     1141 aggtgtgtga cgtccctgca tctgcatgtc cgcccaattg acgtttacaa aagtgggtca
     1201 gcagcagcta acctatgagg aacgtaaagg ccctgataag gatttttaga agaaagatgg
     1261 aaggagcctc agtaacatac acattgtttc aatgaagcgg cgcagaagtt caaatgattt
     1321 aaccttttct tttttctcgg ccagtataac ccaaatcgtt tctctggccc catgctcact
     1381 ccttcacctg caccttatct ccacaaatgg agtgttgact ttctgttctt caaagcagaa
     1441 atactgtcag gctgctgcta atggggatca ggagaggcta ctgttctggg acttgtcaag
     1501 ccctgtccaa cctgccatcc tgccctgaag tgtgggagct atttaacctc tcctgctctt
     1561 tctttctcac attctcccat tttctctctc cccccttttc tgtgcattaa ctcttttttt
     1621 ttctcccttt cttccttctc acgcgttata catttttcct ctcatacttg ctgcatgcca
     1681 tcat
//
LOCUS       KCVB01000769            1447 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36546pri ultra-conserved element locus
            uce-1214, sequence.
ACCESSION   KCVB01000769
VERSION     KCVB01000769.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36546pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1447
                     /note="ultra-conserved element locus uce-1214"
BASE COUNT          449 a          267 c          328 g          403 t
ORIGIN      
        1 tacagatggg aaaacttatc acgtgaaaaa acaaaagact gatataataa tgactggttt
       61 attctaagca gattattgca tggactcgtt tgacaaaagt ttaagtgagt ttttctcatc
      121 aagcaataat atgatttatg tcacattggt attactacaa cataaactga ttattctcat
      181 gaagtttcaa ttaatacaaa ttaaaaagag ttggttgtca ctaaaaatat tattttaatg
      241 tagtgagatt aaagtagaga agaattttct tgcagctaaa agagacaaaa acattttaat
      301 tctcaaagac aattgaaact ggcaaatatt ttacatcaaa caagcagatt aagcctgatt
      361 ataagtctaa aaagtttcac ttacctcaag tgattctttt tttttttagg agggtcaaga
      421 ccccagagct tgaacggagg tgtagaacag gaaggctgag cacgtcctct ctcttttcca
      481 cgcaaacgtc agaggccacg aggcagctaa ccgtgcttgg cagcatcaca gcaacaagca
      541 cgggtgcaag agagtcgtcc ggggcgaggt ggcagccggt ctgtcggagg ggttggtcaa
      601 tcagcccggg tagcagagac aaggggacag gcacacaggg agaagggctg aggcggactg
      661 ggctctgcta gaaggctttt tcgcctctcc ttccctgaat gaaagcaatc cctaagagtg
      721 aatgggtaat gagagaccgg ctcctgagat tttagctttc ttaatcatgt tcataattaa
      781 ttcaagaaca ttgataattt cattacattc tttttgtctt atcaataaat gatgtgcctg
      841 cacttttggt cttgtgtata cgggagagag ggaggaagag agggagagag agggacaaga
      901 gaagtaatac tgcactaaaa gcacaggaga tggaaaatga tacaaacagg gaagtggtaa
      961 ttataatctt tgaccaagcc actggcagca caatatgaag tcaagtggct acaaataatt
     1021 aataaggcct cctggtgtgt gtttgcgtgt ttgtgtgtgt gtgtggtaag ggtaaggagt
     1081 tcagggggag ggtgacaaat gtcagtgttg aacattattt caccagcttt ctttcagtcg
     1141 ttgtttttgc aaaaaagcct gatacaacta ataaacatca ttgtgttttc accaacatga
     1201 tgttatacac actgctgctg taatacactc acctttattc cccacctttg aacagagggt
     1261 gtcgaggatg cctcttacaa tgcccttcag atccaataag ggtttaggtt tcagggacgt
     1321 tagtgcactg aagcagttat taaaagacca ctctcctccc taccctctct cctcttcacg
     1381 tgccctctgt caggtcaagt aaaagaaaaa gacaagaacc atttctgtct cgtcgcatgc
     1441 atagttt
//
LOCUS       KCVB01000770            1508 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36587pri ultra-conserved element locus
            uce-999, sequence.
ACCESSION   KCVB01000770
VERSION     KCVB01000770.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36587pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1508
                     /note="ultra-conserved element locus uce-999"
BASE COUNT          424 a          383 c          260 g          441 t
ORIGIN      
        1 aattgcattt agactcacaa agatattgta gctttgtgca ttgctttcca atgaaaacct
       61 gtaagtcata ccgtattcca agttgtccat cacaaagaat gactctgaaa gcaatgtcct
      121 ggtattctaa tggttcaaat gtttaccatg taactgcaac gtccttcttt caatcctggc
      181 caggaacgtt tgcagcatgt catctatctc tctcgatgta tagcgtctgc tatcagtaaa
      241 ggccaaagtt ccccaaaaat aatctggaaa acagcactga aatcccatca tagctgcatc
      301 acaaacactg ttttttttct ttttaccata cactcgtaca caaatgagca cactgtcaca
      361 ctcagacaca cagatagact gtcacaccct aacacccatt ttttgccttt tatgcagaat
      421 ctactgtaaa ccacacaaca gctccacaga gtaaaacgat gatctgttct cctcccacta
      481 aatatttttc ataggaaaaa atgccatagt aatagaaaca gccaactcct cgctaccact
      541 ggcccagtcg tcatgtttta ggggcggctt taaagcgtgt cacaagacct catgcatagt
      601 aaacagtgcg gcctccatgg ctgactggtg agcaatttgc ctcgtagatt gcctggcccc
      661 ggggccttag agctatgttt atgtatgagc cctcctgttt catatgtatc ggcccccgga
      721 tcattttcta ctccccccct gcccgttttt ttcccttgca gaactgatgg catacacaca
      781 cagacgcaag cacacattta aacacacacc ggccaatgtg cacagaaaca cacacacatg
      841 catgcatatg gacggacaca taacgtaaac attaagagtg atgacctgcg caaggggcaa
      901 cactgtccca gcagattaca ctattgtcaa tacaaataga aatttactat acatgtgtcg
      961 cagcagctgc tgcttaccgt ggcagttgtg ccttgcgggg tgagtggaga tgtattagag
     1021 acatgttgtc tgtgatggtg ttttcacacc tcctcatgta ggctatttta tatcaccttt
     1081 aaccatattt cctaccttaa gtaaacaact tgcactccat gttgaacctg gaaattcatg
     1141 atttgtgagc agtgtcatca gcaccttttc ataacataat cacaaggcca tcatcacgta
     1201 gcattatgta aataaagata ttttcaaaca tgtgtttgaa cattatttga atttcctgat
     1261 gttgatggca tatgctgcaa ggcttcatgc agtgagacaa ttatgtaagt aagaagagaa
     1321 tcagagttca ttaacccttc atcactccct ctgttatccc tccgtcctcc atttcgcaca
     1381 ttcttattcc tctctttctc ctcttgcttc ctctctcatt catcgtttga acccctcccc
     1441 tccccatctc tgtttaatga aagagcagac aggttttaaa gatgcacaaa gctcatcttt
     1501 gagatcct
//
LOCUS       KCVB01000771            1407 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36649pri ultra-conserved element locus
            uce-263, sequence.
ACCESSION   KCVB01000771
VERSION     KCVB01000771.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36649pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1407
                     /note="ultra-conserved element locus uce-263"
BASE COUNT          460 a          215 c          247 g          485 t
ORIGIN      
        1 ttatttcatc tatgaagtat tttatttatg aaaaagcaaa gtacagatgc tatagaaata
       61 ggagtgatgc attagcattt tataatactg agattagttt tagtaatgtc ataaagtatt
      121 ttacaatgta ttggttttaa tatgtttcat agttatatat atttcttacc tatttatcta
      181 tttactatta atcactttgg ggcttcataa aaatgttggt aaatgagaaa acagaaataa
      241 agaatgaccc tgttatttta ataatttgaa ttaagagatg tattaggatt gaggcaatat
      301 taaagcacca tatagataag gggattagtg gatgtgtata gggtgtgttt tctagcaaga
      361 cgggtaatat tattaataat gtgtctggtg tgtttgtgtg tgtgtttctg catctgtttg
      421 tctatgtgtg tgtgtgtgtg tgtgtgatat atgacactgg ccatctgaag gaggtgcctt
      481 gctctccctc tccaagctgc atgaaaggga gctgagaagc ctgatgaatt aaagcctctc
      541 ttcaataaaa catcagagtc tttcagtaac acacgcttga gatgccccag taattgactt
      601 ttggacttta ggatttacat gaaccctcca aaagcagctt acctgtgcag cgatgcccct
      661 tgagtccgca tccattccct gctgatgtgg atttccataa actcatgcaa tcttttttat
      721 tttttctcca tctctgataa atgcaaaact atccactgta gataatttga gggatttatt
      781 gaagtgtttg aatttattta tcttgaaaaa gaaatcttga aaaaaataaa tcttaatcca
      841 tcgatatttg gcacagttgt attaatgtag tcagcctaca gattcaactc aatggctgtt
      901 tattcattta aagaattaac ataagaatta acattactct gattttattt atctacttga
      961 agggattcta cacttgcagt acagtcaatt caacggttta catattaatg tttgacatca
     1021 ttcagaggta gaaaagtttc atcatgttgg attatataaa tcctgcattt tttaaacaaa
     1081 aataacagag aaaaatacag aaagaaaaat acaatgtatc tcatgaaaat gtcttatata
     1141 aataaaaaca ctcgtgatat acctgtcaga tcaaacatta ttcgttgcag tcagtcagag
     1201 tagtccaaga ggctttgcta cccttctgtt ctctcacagc agcttttaaa aagagcagga
     1261 ctggtgcaat gtatgcactt tagtggtcca agtaatattt acttcttata tttcaccaca
     1321 tactttcacc atatatgaaa tatgaattct ggacaggtat ggaagactag aaataacgat
     1381 attacacatc agatcacagt taaaaat
//
LOCUS       KCVB01000772            1773 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36729pri ultra-conserved element locus
            uce-162, sequence.
ACCESSION   KCVB01000772
VERSION     KCVB01000772.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36729pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1773
                     /note="ultra-conserved element locus uce-162"
BASE COUNT          503 a          361 c          346 g          563 t
ORIGIN      
        1 tagaaatgat atatcataaa ctacctattt cttacctttt ttttcatata tctgtacaat
       61 atatctgtaa aatagataaa aagaatatgt gaatattttt ttaatagata ttgtggtttt
      121 tgtaatattc taacaatgtg gtgcattttt gagtttttaa aatggctact gtagctgtag
      181 tggagactac ttactgcatg atatttagca tgtttgactt taatatctaa aactgagata
      241 gccatggtta acttagagct tattagcagt gagaaggtgt tgttagagag atgaagacat
      301 gttgcaaagg acctgcctac ctgagcatca tcaacagcat cctgaatatc aggtttaatg
      361 gagcaaatgt ggcccttttt cacatcagag cctgttggtc agtatccctg cagaaattgt
      421 gcccattttc tcatgtattg accactgatt aaggcatttt ttaggctgtc ttaaaacact
      481 ctggcctctc aaagccaagc atccacacat gtatttgact agacagggat acagcgccac
      541 agacaatgaa tcatgactcc catattttag ccataagcta acaccatatg ggtgcaactg
      601 tactatcaaa ggataaaaaa gaacaaagga tttatatagt ccttttgatc gaagtcccac
      661 tatatggaag tagtatattt tctgtttttc acacatgcac acacagacac gcacagacac
      721 acacacaaac agacaaaaac aggttcaaac acacacacac atgcagtgac agcccaggcc
      781 aatcgatttg ccacgctctc tctcctcaat gctgacgatg cttgagtgga cgggctcctg
      841 gttgcctggt ttacagaaaa gggtccctca aatgaatgta tttttacctc ttgcaggaca
      901 atgtcggata gggaagtaaa taattaaagg aaagggaccc tttttgactg catcaaccct
      961 gccactgctg gtaagctcta ctatccattg tgactccatt tttgcccgtc ctgctaagga
     1021 tgacagtttt cttttctttt tttttaattt aatttacaca catgtgccct ccctgcatgg
     1081 aaacccctca cattaaaggc actgagtgtt tgcatatttt tcttatttga tgtaagtgct
     1141 gacccgatat aagaggaatg ggccatgtgt atgtcgtata tgacatcgct cattagttgc
     1201 ttaagtttcc tttctgtgtt atgcagtttt gttgtgacat gtgtgcagtg ggtcttcttg
     1261 ttctcagtct gacttggcaa ctgtgaaatt ggagcgaaaa agcattgaat tagattttac
     1321 aagcccattt caccttgcat cctttagcat ggcttggtat gctaactgaa atcctcacta
     1381 atcctatcct ggcttaagca tggcacttgt tacttctttt ttatcagctg tagcactctg
     1441 tgtgatacag tttgccttgt gtttcggaaa aaaatgccca tcttaattaa atccagagct
     1501 acataaagac atgttcattg aggtgctcga ttaagccacc tccaataggg tgcgaatgcg
     1561 ataatgctgt cagggcctag tgctaatatg ctatccagtt cccctcgatt atcacattac
     1621 cacagctcga atcaattgat aaactcccat tcaatatggt ggagtattta attgagtcag
     1681 agtttattgg atttgagggc tgtgcagtgc agacatgctg acagcggtaa acaatagcag
     1741 gatgctctct ctctgtgcga tcttagagct gca
//
LOCUS       KCVB01000773            1497 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36776pri ultra-conserved element locus
            uce-921, sequence.
ACCESSION   KCVB01000773
VERSION     KCVB01000773.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36776pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1497
                     /note="ultra-conserved element locus uce-921"
BASE COUNT          416 a          286 c          361 g          434 t
ORIGIN      
        1 agatgattta attgcatctg cataaaatcg tacatttcat tacacaaatc cagcctaggc
       61 ctaataaaaa aaaatccaaa aagtcaaatt atgagagtag atgatcctgt ttaattgcag
      121 tgaatttcat cacttaatga tggtattgtc tcactctata ataacagaat ctctatgcta
      181 tttttctagt ctcgcaaatg agggaatttt tgaggtttat gtttgcagaa gcccaggccc
      241 tttgttttgg tttttctgta tggggtgtgt tagtgctatc aaaggctggg tgtgtatatc
      301 tctgcatgtt agttaagcag cttgtaacat tcattttacc catagcagta gaggagaggg
      361 ataaaaaaaa atatacatgg tgtgagagat gggcagacag ccctgagagg acaatagctg
      421 ttggcttgaa aatgcttctt gattattaaa agctaatgca ttttcagcat tgcagccttc
      481 attaagggtg cgggggtggg gggtggtggg gggtgttagg gatgagaggg tactgggagg
      541 aggagaggat gggagaggga tagagagaca ggggctgagt ctgtcctata actcctgggc
      601 ccaagacaca tgcagctaga agattggctc gggcctctcc taggggtcga caatgtgtca
      661 tttccacccg gctagattgc cctctgccta tctgatatta atgtgcaaat caatatttca
      721 gggattaaat ttcccttctt cattttttat ggtttctacc tcaataagtc tcccgtctaa
      781 tagaggaggc tggatgattt agaagccttt gctgcagaaa gagagagggg agagaaagaa
      841 atagagagga gctttgcaac aaggctatcg ctgcatttta atgttcttct tcgtggatcc
      901 cccataggcc cttagtaagg agggctatat cgctgcttta cagtgtaaag acagccaatt
      961 tggctaaaaa ggcagaaagg gaaaatatga gtccggttga atacttaaga cgctgtggta
     1021 atccgagtag caggtattag tgaaggggag ctccccccct caaagaccca cacacacact
     1081 gagctacatc caaacctgca ttaccagccg tcatgcttga gcgaaccgta tagcttacta
     1141 cacgccggac tgctataaca atatattttt ttcctattct ctttctcctg cacatttcgt
     1201 cttttttgtg tagaagcaca tcgacatcca ttgaagagat ttactctcct gcgttataac
     1261 atctggcgtg tgtgtgtgat agaaataaat cactctgaca caatgacctg cgtatgatta
     1321 ttggggaagg gaggagcata ttaccatagt cataatgctg catctcattg agagtcaact
     1381 aaatgagctt ggaccctttg actccccata gacattcatt aagggacagt gcacacagag
     1441 gcgtctgtct gccgaaaggt gtgtgtctga gcatgtgtgt ttatttaatc ctccttc
//
LOCUS       KCVB01000774            1460 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36837pri ultra-conserved element locus
            uce-672, sequence.
ACCESSION   KCVB01000774
VERSION     KCVB01000774.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36837pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1460
                     /note="ultra-conserved element locus uce-672"
BASE COUNT          434 a          393 c          313 g          320 t
ORIGIN      
        1 ctccttaggg ccttgtgctg taagttataa agtcaatgtg gatggtctgt gtcaagaagt
       61 ccatccagct ctctacaatg ttgcgacctt ttgcccgcca gtgtaaaagc tacctgcagc
      121 ctaatcaact tggcaggatt agctccgaac ctgatgactt ccactgccgg cagctcagac
      181 accgaggaag gagacagaaa gagaatgaga aaagagaaag gtaaaaacag gaagaaaaca
      241 gaagagtcac agaaaataaa accattcaac cttctgagac taagacgaca atggacaaaa
      301 ccgtaatggc aaaaacaccg gagcagaact caacaaaaga ccagagaatg agaaaagagg
      361 cagaggaaac tgtgaggaaa ggattcttta ggcctcttga caaacatgtc aatcatttta
      421 ccctaaccga cacacacaca cacacacaca aatgcacaca cacttgcact ttcagtgctg
      481 aagcaaaatc taattgtttt tcagaccggt ctagtttgac agtcgacact gcaaccgtgc
      541 tccccccaac cttcaccacc gctgagagag aaagggaaca accggaggtc ttttattaga
      601 tatgatggca catttcacag ctccctgact tacagaaacc caatcccgcg attagtcagc
      661 gctttgacgt tatcttaagt tccttgtcag aattaattta gtgtgggtgg gctgctgtgg
      721 ggctgtctgt ctgtcgcgct ccgctcccct ccgctcgcct acaggaggtg ggggggttgg
      781 aggcaacggg ggcagaagat ccctttcaaa taagccccag ctccactctg tgccaaacca
      841 gccgcctgag aactaaatat ctcctccatc aaagggcccc tttctgcaag ggaaaaggag
      901 tgaatgggct tgaatgaaca ggctgaaaac agatcccgtc ctggcccagc ctgtcgccca
      961 cttagtgccc tcagccctaa gccccccagc cacccggccc agccaatgat accggagaac
     1021 cgatacagct gatatactaa cttaacacac gtgtcacgca cagctaaaca caaaatcaac
     1081 tacatactgc aacgacagta tttttgttca ttgaaaagga acattgcacg atattttact
     1141 tatactccgt ctgcttgtac acatcagaaa tcctggttat cagaagcact cacacacgca
     1201 cacacaagca gacacacaca gaatcaaatt taaaagtatt gatattgtac cctgatcaag
     1261 attggcaggc gactagtgag agccatttgc cttgccagtg tgaaactgga ggcctgtctg
     1321 cactctccag gcctgtgctg acacttcatt agaggtcttt tttttctggt ggccctgcac
     1381 tccctggccc aactccccac ccaactctgc accgtgccac tgtcccaact gatgaaaact
     1441 gatggattac acggcatttc
//
LOCUS       KCVB01000775            1440 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36912pri ultra-conserved element locus
            uce-1229, sequence.
ACCESSION   KCVB01000775
VERSION     KCVB01000775.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36912pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1440
                     /note="ultra-conserved element locus uce-1229"
BASE COUNT          447 a          323 c          287 g          383 t
ORIGIN      
        1 ctctgctaac cctcccttcc aagcgtgtac gtacagttgc tgcgaaactg gctccttttg
       61 agccagtgtt ttgtttgttc attctggtct actgtggaaa catgtctgta aaacatggtg
      121 gactctgtgg aaaaggaccc tgctcctgtg gttttcttac tttttgaaaa taaactagaa
      181 attcttaaat tgtgtctctc tttgtaaaca cagatgtatt tgtacaaaca tgaacacagt
      241 gacacaaaca cacacagcac cacaagtcaa caggttgcat cctttccatt ctggagacaa
      301 agggacgtgg aagaaaggcc tcaacatggc gatagcaatt cattgtcacg caaccccaaa
      361 cacccacccc cttgttttac cactgtcagg gttgatgccc tcgtctttcc atacggacct
      421 gataatgagc ataacctctc ccttctcatt catatcaggt cagctctgtg ttcacacact
      481 cccgctcact ggctaccatt acggttccca aaggtccatg acaactcctt tgtctctgat
      541 gggcgagtcg acaaacaatc tgcgagcgcc ggagttcaga aaaacactga tggaaatgca
      601 agaaagcagc gagagtgaga agagagagag agagagggag ggagagagag agggagacag
      661 aaagacaaca actgacatct cagggaattt cacagggatg gctaatccaa tgtgatcagc
      721 ggaagatttt aatatttaaa tagccagcaa cgcaccagaa gtttctaatt aaattataac
      781 tcatgcttga aaagcaaaag tgcatgtgtt gtgggagctt aaataattca gcccgcccta
      841 tatctcgtct ttctgttaac ccggagacaa atggcacttg ctagggcttt tagacagggg
      901 gctggcactg tcagaaacat gaacaacatc agcttgtaaa gggcctcacc catcagggcc
      961 caaatccaaa ctgactggcc tcactactga acaacgtttc tagcctagga attaatttgg
     1021 ttaattattc attattaatt accttaaagg ccacacaaca ttggcacatt gtaaaaagat
     1081 gacgggaaaa aagcctcttc ctaatgaacc ggggctgtta atgaggcacc agacaactct
     1141 gttctggctc ctctcactat atcatcctta cctgctcgct cttattacaa gtttccagca
     1201 aaaaaagtat cagaaaagag tttattaatt aatacactct tgaaggatat atgagacgct
     1261 cacaatatcc atgagaagcg taaaatattt agggtgctaa cattcatatt aatggcaaaa
     1321 ttacacatac atctcagaaa ttaaattaga attcgaccca acattgatgg cagagtcatg
     1381 tcaagcatat aaatgtactt atggctcgag gttagaatat ctcttttcat tcattatttt
//
LOCUS       KCVB01000776            1318 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36950pri ultra-conserved element locus
            uce-573, sequence.
ACCESSION   KCVB01000776
VERSION     KCVB01000776.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36950pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1318
                     /note="ultra-conserved element locus uce-573"
BASE COUNT          467 a          294 c          274 g          283 t
ORIGIN      
        1 agcgaaagca ccatatgatg cacctaaccc acaccccagc acccagaccc ccatgcaccg
       61 ctgatccata tctggacctc acccccctga tccctctcat ctcttgctac aaggaagaga
      121 aaaccgacct gaagatgaac agtgaagcca acgcagacaa acataaaaaa aaaagtgtca
      181 gctctacagt agtgagcaat gaggggaggg agggagaaat tgcataaatt gaaaaagagc
      241 agaatgataa gagggagata cactctggcc cctcctctag ccttctttta gggcacctct
      301 tggcctcctc tccctccgcc gcctcacact ccctcctccc cccacccagc cgccccgggg
      361 ccctctggtg tgttgctcac tccattgtgt cagccttcag aagtgctcag tttgagtggc
      421 aggccgtcac agatcagttc cctgctaaag cggcccggca ggccggggtg acgggccagg
      481 aagggatcta agtggggttt atcagcgctt cttaacgcct ctccggctcc ctgacacaaa
      541 agagaggggg tattaaacat ctaaagcaaa ccaaaaagcg tgaaagctcc ttatagggcc
      601 gcgggattgc ggatgtgact ggagtcttta aaaaatagca aaaaaaaaaa aagaaaaaaa
      661 aggaaaaaag aaaaagaaaa agaaaaagaa aaaaaaacac acaaaaatgc ttcagcagtg
      721 aggaagcact gagggaaaat tctaatgtat tttctatcct gtgtggatag aagagtgggt
      781 gggtgagaga gagagaaaga aagaaagaga gtccatgtgt agattgacaa aacaagcgaa
      841 acaaagtgaa aactacctta ataatccccc atttatctgt tagcaaggct aaatgtaaaa
      901 gtcatatata tcaaactagt ggatattaca ttaaaaatgt accatttcaa aagtacttat
      961 atgtaagcat atctaggcta gtcaatgaca atataaagac tactaacgtt cagtaaataa
     1021 tttgagctgc agtggtgtta aatttcgaca ttcaaatctc aaacacatac aaaacggtct
     1081 caaaacctac tacccaactg tgatcatatt acagaattaa ctcaacgatt ggggttggta
     1141 ttagaagaaa aatcgattct tatctcctgt gattcagaat cgattcacga aagttaaaaa
     1201 aaatcaattt tcaagtgtta gttaataaca tgatgttgac gaaacaaaag agacagagct
     1261 caactgcaaa gacagctcaa cacctggaca gcgtccacac gcagaaactt tcaatatt
//
LOCUS       KCVB01000777            1639 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 36996pri ultra-conserved element locus
            uce-1190, sequence.
ACCESSION   KCVB01000777
VERSION     KCVB01000777.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="36996pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1639
                     /note="ultra-conserved element locus uce-1190"
BASE COUNT          441 a          377 c          380 g          441 t
ORIGIN      
        1 ataattggat gtgttgccat tgaacgaaaa aaataaatac tttttgcaga atgactatgg
       61 ttatcaaaac atttccaatg cgatcatcat ttgctaatcc cacatgtctt ctatcttgta
      121 tccgtctgtt cttgttgttt acaccccctc ccgcccccgc cacaccctct gtcagggtgt
      181 gctttaacaa actggacacg ttacccagga gtgttgccgc tcaccttaca gcttgcaggc
      241 acatctgcag gctgtgctgg gacagcgatg ctagccagca ataacacagc aaggacatca
      301 aaggcgggca gcttgggccg caccacctga gcatgttgat tgcaatgcac cgcagtgtca
      361 cctgacctgg gaatggagag gattttgccg tgccaagtgg aggcattata tccaggatgt
      421 gctaaatatg gttgctacca gagcatacag tgttagtaat acagcttggg agcagtgttg
      481 aaagatcaaa ggcttttgga aagatatatt ttatttgttc aaaatcatat gttcttgagg
      541 atgaataaat tcaagatttt tgactcttta atgcaagatt aaaatatgat actggctaac
      601 tcaagcatca ggttgacaaa atcatgttag ataagttacg aaatatgctt cccactaatg
      661 cattaatctt tcctgaatat taagttaagt gtattttgag catcagtcaa gcatacttgg
      721 tgagcctcac agggactctg tttatgttga cacatgcaac tctcttcctc atgcaaatgc
      781 aatctaaaca ttaggcaaag ggaaggcaag cgtagcccat aaaggctgtg agctccaaac
      841 actttgaaca gacaactatc actaatcact cccagagcag tgcggcgtgt aattactctg
      901 ccaaacgttt gcctttcaca gcttagcacc gggagccctg accctgcgct tccctgtctt
      961 tcctcaccac catgcatgtg gaaggggagg aacccctctc cctccctccc cttccccttc
     1021 tcttgaccga gggggaaggc aagctgagct ccctctcaat atttagagaa agagtggagg
     1081 aggaaagaga gagggaaaaa gacagggagg gagagctctg aggttggtgc ttccggcttt
     1141 gtgggagccc gtttaaacag gctttgaaac cctagccatc cggagatcag aaatagcctt
     1201 attgactgca tgggcccaga agtgactgag cacacagggt tcttagagtt cacccttcct
     1261 caccactcgg aacaggaggg gggcatcgca tgctagggcc atctacaaag gctacggaca
     1321 ttaattttac atcaggagaa aaaataatat tgctctggat atttttagtg tcacaagatt
     1381 gtgccatcac ataatttctt tgagattttt ttcgcagggt gcaggacgtc taattctcac
     1441 tctcctctgc ctccgtctta tttactctta ttagtgatgc ggctgtcagt ctgcagtgtg
     1501 atctgtcctc tgtctgggtg aatgctgata agatgacaca atgcaaatat gaagtgtgag
     1561 ggagccgcct tggcaagcag gtaggctgtg caaggaccgg gatcccataa tgactattag
     1621 ctggatttca cagcctgct
//
LOCUS       KCVB01000778            1630 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37034pri ultra-conserved element locus
            uce-1334, sequence.
ACCESSION   KCVB01000778
VERSION     KCVB01000778.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37034pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1630
                     /note="ultra-conserved element locus uce-1334"
BASE COUNT          421 a          427 c          355 g          427 t
ORIGIN      
        1 cgtcagcaga tcgcagcgtc ctcgccgaga aaacaaggca gcaccgagga aaagggggga
       61 atgaacatgc accactccgc ccaccaaacc ccaggctctg cttttttaaa cccgcgcctc
      121 cgcttcagcc cgtgtcccgc ccgctcccct gctacccccc ccacccccga ctcatccacc
      181 ctctacttgc aaccctaggc catgagtcca ggctgtgcat gacagctcgc aacatccctt
      241 gccacaaatg ttttcccaaa gcatccactt ccgtgaccct atcagcagat aaaacctccc
      301 cacaacccct gatggagtgt gcctctcact tttctgaatg tgtcaacaat ttctggctgg
      361 ctggccagac actgcgtcct ggcgatgtct gttgtgtgtg tgtgtgtgtg tgtgtacacc
      421 cccgtgtgta tatgcgtttc catgtatatg tgtgtgtttg tactcgctta tgtatgtttg
      481 aatatattta catctttgtg ttttttctgt cttttcctat ataaagcagg aaaaaaaagg
      541 gccatgtcat tcttccataa tttaatcagg agctacacac aggagagggc tgctgcagca
      601 cggacctccc tgatcatgtt aatgaggtcc cctggctatg cagtcattga agtataattg
      661 acttgttcgt ctcttatctt cctaatagac actctgaccc agagcacgtt tatttcgcta
      721 tgagaaatgt caagaaggat tggaagtttc ctcggtgtgc ccattaaaac tgaaggcatg
      781 ccaccctgtc tccagccatt cagggctgcc attggctccc tgcctcatta tgcagatgcc
      841 aaacgtaaat aaatgatttg cagatcactg ctgcccccac cattctcccc ctgcactcac
      901 cctcttccca aactgtggtc ttctgttctc tcccaggact ggaaaggagg acagaaagga
      961 gggaggaagg aaaggaaagc acgaaaccat ccagggagca ccatgacgat ttgtctctct
     1021 ggagcacgat gaataacaca taggtagtaa ttataataag acatgtccct gacttgaaaa
     1081 cctggcgaag cgcattgatg ttgttacagc cacgtatgtc tcctggcttt caggacccat
     1141 cagaatcaaa catggccgtg cgtaatgtgg agggaccaag aggcaagctg cgtaagcggg
     1201 cgccaggcga aggcgaacca gtgactgtgg tcacatgctt gtggatatga gggggaacat
     1261 ctgtgtatat cagcagccta taccctccct gcatgtccac cgcatcttct cgggccagta
     1321 gcagagtcac agagaaattg cttttggtta caaagcattg ttttgctgaa aactgtctac
     1381 cttagagaag cctagtttct gcctgttaca gcttccatat ctccaccccc ctgaaaatcg
     1441 ttgttctgca ttctcagttt ttggtttact actttatttg acaggggcag tgcaattcac
     1501 tacatttctg aatatctacc agagttagcc aaaaaagaaa ttttaaatgt agtccctggg
     1561 caggtaattt aaagacttag atgtgtagaa taaaggcatt gaaaatacaa tcagcaataa
     1621 aaacaataaa
//
LOCUS       KCVB01000779            1023 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37080pri ultra-conserved element locus
            uce-343, sequence.
ACCESSION   KCVB01000779
VERSION     KCVB01000779.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37080pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1023
                     /note="ultra-conserved element locus uce-343"
BASE COUNT          281 a          199 c          245 g          298 t
ORIGIN      
        1 attactttag aaattaaatt tactgcacaa cagtttttgc aactttatta catcccagtg
       61 gtagcaggtt gacctttttc atcaaacaat gatatgctgg gttagagaga taacagatgc
      121 tgtaattcaa gaattcagtc ctcagaaagc attcgattta tgtcaagtga aatctggaat
      181 caaaactatt ttgacataat gactatttga ccttttatct agaaaaatga cattaaaaca
      241 atgaccgaag cttgataacg taaggcacat ttgcaagtaa tatattagga aatcccctaa
      301 aggtaaatac ttagcacagt aaatgtttta attgttacgt ctataggtta tttgtgtgtc
      361 attgttcatg ttaggacatt agagttattt tggtatcttc catcaaaaca ggtataggtg
      421 tgtgtgactg tgtgtttgtg tgtggcgggg gccctgagtg agggccacag gggcaggaat
      481 gtaaaggctg ccctgggggc ctctgtggtg ctaatggatt aacaggccag cttggcatgc
      541 taatggagat tgtctgtgat gtaatgagct tctaattagg tcacctctga gcttgaccct
      601 cttgtaatga ggagggcaga agggagagga ggggccgggg ctggtgatag caccctgacc
      661 tgcaggtgtg aaccggcccg ggctgtaaac agggctggac attcctggca ttctcagctc
      721 agcatcccct cccgaaactc ccctcctcct gcctgccaat aacccttctg aagcgctatg
      781 ggtggataca cacacagaga aacaattgga gccacatttc atgctccaag gtgcttcccg
      841 gtttgtctga aaggaaaaaa acgttccctt gagctcggct tttctttgat tgttggacat
      901 tatttttctt tatgatatcc ctctcaaagg ggtgtcgcag cattatgctg tgacctggat
      961 tcaaatgcta aagggatctg caggagggga actatctaat taaaagtgta agactctaat
     1021 cac
//
LOCUS       KCVB01000780            1694 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37121pri ultra-conserved element locus
            uce-792, sequence.
ACCESSION   KCVB01000780
VERSION     KCVB01000780.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37121pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1694
                     /note="ultra-conserved element locus uce-792"
BASE COUNT          485 a          369 c          418 g          422 t
ORIGIN      
        1 tacgctcgca cgcacaccca cccccccagg tcaaggccct atgggggtct gcacagaaat
       61 aggtcatttg tcacacatga tgtcccgggg caggggatgc attatagatg agcccaaaca
      121 gcatgtctca gtgagcactc accacacagc tagacacagg aggaagcagc aggaggtggg
      181 cagagaggga tgagaactcc tcaggaaggg gatattggga tgtcgtaaag taagacaaaa
      241 atcactgccg gccacgtttt taatcataaa gcaaagaaaa gagatcattc agcacagcct
      301 gatcagagct acgactgtac ccttctcaac ttttcactga atagacatga gttaattcag
      361 ggggtatcta atttgttatt ctcatgcctg cacctgaccc tatagctagc catctgcctt
      421 gcatatgaga ggtccagaaa tcattcagtc attagtcgta taatgaattt aatgcaaaac
      481 tcactcccaa tcattgcata ttgtgtcttt tccacgactg ctttatttct ctgattgtca
      541 gccgagaatg tggactcaga aagtctgccg agctacactc aaaattgctt ttatttgcaa
      601 agacaaaggc ccgaggagtg aagttgtttg ctatggtttc aaactggcct aaatgggaaa
      661 aaaaagagag aaaggaaggg gctgagctga gggagaagta agtgcgaaat gtggggaatg
      721 agcgagacaa gtctgctgtc ttctgctgca cccatctgtt ctgaaagtgc aggtggagcc
      781 ctattcactc cgctctgcat gtgtgtgtga agaggggggc aggaagaaag gaagggcagc
      841 gagaaaaagg gagtggagag atggaagggc agggatggaa gagggagggt ggtggtggtg
      901 gtgggggggg actcttggtg atctctcctg aaggggataa tgggagagga gcgcattgca
      961 atggctgcca tgtagtaccc tccctgcaca attagccaat cagcagcgcg ctctgccagc
     1021 caggaggacg cataaaagaa gaacattgca gcagaggcac agaaggagac tgcgagtgga
     1081 gcagggaatt acacataaaa acagcagaac cagaacaccc tcccctggac acaccctgct
     1141 gaggatcact gcttctcttt ttttctgaac catcgcccac accactcaga gagaacgctc
     1201 gacctcatca tcatccatta gaaaacccct ttctcctcat tttcatccta tagaggacac
     1261 ctacaatctc agagggctga gattccctgg cgaagattgc atttttttcc tttttttctt
     1321 ttttttttct tttaatttgg actcctgaca gaacataagg gccatataat ataaaggccg
     1381 aagagcaaac agaaactcag ccttcagcta caagaccagt gcaaagaagg acctagattt
     1441 ctttcattgt acgtcaagaa tggttactgt acgtatgaca ctttctctta tatttgtgaa
     1501 ggctatgttg tagcagcatg cccagattgt atctcagtcc cttttgttgg atttgttgtg
     1561 cactataata catgtacaca tgtgatgtag ccttacactg atttatttta catcttactg
     1621 gtacatctct agtgtagact actataaatt tgaatggagg tccatggcta tgctcacatg
     1681 ccaaaacata gagg
//
LOCUS       KCVB01000781            1207 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37160pri ultra-conserved element locus
            uce-306, sequence.
ACCESSION   KCVB01000781
VERSION     KCVB01000781.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37160pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1207
                     /note="ultra-conserved element locus uce-306"
BASE COUNT          374 a          260 c          248 g          325 t
ORIGIN      
        1 gagggagaga gagagaggga gagagagaga gagagagaga aagagagaga gagagagatg
       61 tgatcaatgg tgctgtcaca taagataaat tggaaagaaa ccaaaaaatg ttttatgtca
      121 gtaatctagg actcctgtag ctgtagttag ggattctacc atttgtttaa agctgataaa
      181 gctcctgcaa ctccagatag cgctaaaacc tctagcagcc atttctcttt cctgtgcagt
      241 ttgccaaaat attcttgaaa aaccgaggcc atcacattag agtcctgtgt cactaatagc
      301 tatttctctg agttgtgcag tttagcagac tctccttttg cctttattga acttcatgct
      361 agaactatga attatgaaag cattagcaaa gtgtttttga atgcaagggc atttagctgg
      421 acttgttcta caagagggac ttacttgaca tttatgttgg cacagatgag tacatcatca
      481 aagaggaaga cctttctctc cttcgacttc aggacttgtc cccgctcgcc atacacagtc
      541 tcaatgagcg tctcacaaag gaccagtgac ccctgctccg agttcagttg ctaaaaggga
      601 cacagcaatc agatcagagg cggagagcca gtcctccaga gttcagtgcc tggaggacac
      661 agaagtcagg cttggaggaa cagatgtctc atcagggatt agcaacacat tcatgctgct
      721 gaaggtcatt taatgtgtgt atacgtatac acacacacaa gaaacacaca catatctcta
      781 gcaatttcat gcagcaaact gagtaaaaga gatattgcat atttgccttg cacatttaca
      841 gtcagggacc caaagacatc tgaaaatgta ctggtgctac agtagctagc agaaaccttt
      901 attttattta tttatctttg ttaaacacag ttgttaatac ttccaacgca tgcttcagat
      961 cattctttgt ctttttgtct gttccatgct atccaccgta ccactaccac agagtctaaa
     1021 ctggcttagc ttattcgctc tgggacacac aatcctttcc accacaacaa aggcaactac
     1081 acatggatat aaatgcatga atagtttgct gcatggacac ctttacacac atgcatccac
     1141 acacacaaac atggttaaag aatccaataa cagatgcaat ttattcaacc gtagctggcg
     1201 catgctg
//
LOCUS       KCVB01000782            1131 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37203pri ultra-conserved element locus
            uce-1035, sequence.
ACCESSION   KCVB01000782
VERSION     KCVB01000782.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37203pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1131
                     /note="ultra-conserved element locus uce-1035"
BASE COUNT          322 a          229 c          218 g          362 t
ORIGIN      
        1 tggggatgtc attggctcag atcagctccg gccttgtctc tgaccgggga ttctcctccc
       61 ttctaatgaa aatgtttcat tcacatctca catctcatgc ctccctcgct gatgaattca
      121 atagaagaaa aaagagaaac gaatggtgag ggaaagaaat tcagcttttg tcagattggg
      181 gaggcaagaa gagaggatga cccctgagcc aaaggttctg acactgcatc aatcccttta
      241 ggggtgaggc ctgctggacc agctctggct gaagattact tcagcacatc tgagggatgg
      301 ctgctctatc tatttcacac attctatgca gaggagagcc ttgtatgctt tggatcctga
      361 gctgcccagt aaattaggtc tgagatcatt ccatggaatg aggggagcta tccagggcaa
      421 ttcaactcca aaccattgac agagagctta ccgaatccct gaaaaaaaaa gggagacaca
      481 tctagtccct ctttctcttt atgataccaa ctagaatgta ccctgggtaa tcccaataag
      541 gctctttact tgcaaattca agtatatgca cacatgtacc gataagacgc tcccacgcag
      601 ctttctcaca ttcgtgttat tgacaagcat atcacatctt ttcagctctc ggcacttttt
      661 gtcccagtct gaagtaatat ctttgacttg ttaggtggtg caatgcttaa tatgatgtgg
      721 taataactgt taattatcat taacatttca gacttttggg ggtgtcagaa aacctctggg
      781 tcatacagat tgcttagttt tttattgcta tgttacccag atcaatgtta tttaaagaaa
      841 ggataatgta atatatattc taccgttttt cttgattgtt taattattct tcatgttcac
      901 taacagcact tcttggtata agtgtgtaat aacaaagctt cattatggtt tccttgtctc
      961 tgttttcata cagttttatc tcaaacttta acttaatcta aactggactt taaagtagaa
     1021 aaaaagaaaa aatctcccag atctcatggt cttttcaaac ttgtgtttgt ctgcatctga
     1081 taaagtgcaa tttattcact gctgttttca ttgagagata tcaaattaat a
//
LOCUS       KCVB01000783            1530 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37271pri ultra-conserved element locus
            uce-493, sequence.
ACCESSION   KCVB01000783
VERSION     KCVB01000783.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37271pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1530
                     /note="ultra-conserved element locus uce-493"
BASE COUNT          429 a          252 c          310 g          539 t
ORIGIN      
        1 atgacagtga tacgatgaga cctagtttca cagcctcctg tagtgtgcag acaatcaact
       61 aattgccgga acttttctca tgtcaacagc cacaagaaga accaactgaa tccgctgata
      121 gtaacaatta gttacaatta tcatacttag tttttctgtg ttaaatcagg aataaataaa
      181 taaataaaaa taaaaatgat aataaagtgt cataatcaga cacgcgtatg ttctgcctgc
      241 aatgaaatta ttgttgtccg tcatgtattc accgacatat aaaagcaaca atttctaatt
      301 ttatcacact ttgcgccatg tgaactgtta aaaataaaag gcaaaattag tgacattatg
      361 tattttattt attcatttat tgcatgaaaa ttgtgaccaa gtttctcgag gagagagtca
      421 tgtgtcctct ttaatgcgtt ttggttcaga gttgctctga agtaggagga ggacccataa
      481 tttgagatca cggagcctat aataggagta ggttatggca actgcttcca cctttcagca
      541 ctgcactgat aaaggaggag gtgtaggtga gtatagtttt gctcatttgc gcttttaatg
      601 tggaaaggtg cgtatgtgtt ttaggtgctc taaaagtggg cttttctcac gcaaaaaagc
      661 ttttccgaat tcttgttttg acggcttcta actttgtcgc ttcctcttcg ttatcgctca
      721 ttcttttttt tctttctccc cgagagaatg cactcgggtt ttgacgcatg aaaacggctt
      781 tacgtggcaa agaagaagtg aatgaaagat gatgcgcgtc acttaaaacg cctgagggag
      841 caagtgtaat ctaaattgga tatagagggt attattacac aacattatgc gttgtggcgg
      901 actaattgcg tggctgggtg agttaaacag tcagttaatg tcgagtggcg gacaggtttc
      961 tggagcgcac aaggtcagct gcactgctcc caagccttta gtggtataca ggaaaaatgc
     1021 ttgaagtatt catgggattt ttaaccattc taaatctgtg tcctaaaccg attagttgac
     1081 accaattttt ctttgatgta ttagctgagg gcttttgcca cttggaagcc aattgaaatt
     1141 caaaacagtt ttcgctccaa atgctttggg gagatctgct gcaaataatg gatgtaattt
     1201 atttttgacc ttgcattttt tttctttttt tttttgaaca gggggttatt aaatgattaa
     1261 acatatgtgc tctatgctgc ttgactaaac aactggcatc tatacatata ttattagcta
     1321 ttattctgaa agtaatttta cccgttttat ttatgcattt gaaaatgtga caggcatgac
     1381 atcgattgtt acctagtttt tgtgtatttt gtgtgttttt agtcctgtaa aagagttgtt
     1441 tgtttttttt ctttttcttt tttcccaaat gcattaaaat aatatttcac tgtttgtttg
     1501 tctttgtttt gaattggttc agatccatgt
//
LOCUS       KCVB01000784            1229 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37314pri ultra-conserved element locus
            uce-46, sequence.
ACCESSION   KCVB01000784
VERSION     KCVB01000784.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37314pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1229
                     /note="ultra-conserved element locus uce-46"
BASE COUNT          387 a          250 c          263 g          329 t
ORIGIN      
        1 attaccactt gttatctaca gataagccgt ctgacaaatg gtgactgaca gcacctatta
       61 gtcttataca tgcacaatta tatctgtgca ttaatctttc taagttgttg ctgcactgct
      121 gggaaaagca cccagagaac caccgtgcag cataaaaata taacaaccat aactctatca
      181 ctctgcattg tcctgatctg tgcttttata tcaatccttt ttttgtgtat ttttttattg
      241 ttcactgata tacacagaca agaaagacat atacgcacct cctcccactg gtgtatgtat
      301 ctgatgagtc gggacagtct caacagacga agaagactga ggatcttggt gaagcggacg
      361 attcgcaggg cgcgggctgt tttatatacc tccgagtcaa agcctttctc cactattaaa
      421 aagatataat ccactggaat ggaggagagg aagtccacga cgaaccagct cttcaggtaa
      481 ttcatcttga tgaccttggg gtcgaggatg atctcggagc tctcctcgtt gacgatcccc
      541 gtcctgaagt tcatgaccag gtccaccagg aagatggtgt ccgaggccac attgaagacc
      601 agccaggtgg tggtagtctg ctctgagaag aaggttattc ccacaggaat gatgattaga
      661 ttccccatca tcatgatgag cattatcaag tcccagtaga acctgcagag cgacacacat
      721 gagtcagtaa tgtaaggaat aaaacatgct gtagaaagtg aatacatacg gagtgtattc
      781 acatacaaag gtgtaagtgt attcacattt ccaaagaagg ggaatgcact tcgaggggtt
      841 ttgcctggca aagagtgatg caagatggca ataaagtaaa agaaaataaa gcagatacag
      901 aatgtacagc gtctctgcaa ggcagtgcag cggtgcttta agacaaatgc tcacatcagc
      961 atgctacttt gctcacaatc aatatattaa catgctgatg ttgagcaggt ataatgttta
     1021 gtatgcacac catgataatt taatatgcca gtatgctaac ctttgctaat tagcacttag
     1081 cacaagtacg gctgaggaat atattagttt tgcatgaatt tggtcataaa tccaagcatt
     1141 tgaagctcaa caatttatcg accatgttag catgcaggca tttgttaatt agcactaaac
     1201 acaaagtaca acaaaggaga tcataaaag
//
LOCUS       KCVB01000785            1765 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37356pri ultra-conserved element locus
            uce-801, sequence.
ACCESSION   KCVB01000785
VERSION     KCVB01000785.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37356pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1765
                     /note="ultra-conserved element locus uce-801"
BASE COUNT          522 a          356 c          390 g          497 t
ORIGIN      
        1 tgacttcagc ctgcagttgc ataaataaag ccggccgcac aaaacagcag tccttaagag
       61 cttttgtgtg gccgtagagt tttatgatcc taattctttc gcatatattg ccaacataaa
      121 tatggcactt caccgtggga tgctccacgg agggccgtta agaccatagg cttggcagca
      181 acaggttcgg atacacaact tttactgcag gccctatgaa tttctcccat caatgccttc
      241 tcggcatgtt tgggtgtgag cagaggacgc atactgtgta tccttaacaa aaaaactctg
      301 atgctgatgg gagatgattt gaagcttaaa cgcagggaag aattcatcct tttccttttt
      361 atatttaaat ttttctgtgt tttagaatag atgcacccaa cacagtggag tgaaagatgt
      421 ttcttgattc aacctgcaga acctgaactt gatttctcca ctcagatcta aacaaaatcc
      481 tgacaggttt tttctttttg actgatgcaa ttttgtaatt tctagacact tctgttcagt
      541 aaaatatact gcctaaagat tctcgctcag tgcatgccat aaacaagaaa tattgtgtta
      601 tgaaatttga aaaacagcga cataaaaaga ttaagattcc gctttatcca aattatttaa
      661 gatctcaggc tacaagaaaa cagaatataa gaacatcaag ggctgtgatg cacaacagga
      721 aaaaggcagt tgactatatc atcataaccc tttggcaaca acactgtggt gggtgattca
      781 gaaacatgtg ctgccatttt atcatgccga gggccaagtt ttggagcctt aaacaccccg
      841 ccaaggcttg ataaatctgg cccgaaaaat ctcatttgca gagagttagg catctgcaac
      901 aatatttatc ttcttccctg tccaagggta atgggttagg caggatggat gagggcacag
      961 tcctcttgcc ttcaggtgca gcgatctcac agaggacccg tagacaggct gcttatgacg
     1021 aagcaaaagg aaacacatca actccctcag taaacatatc accatggttg aacaattggc
     1081 tattagcata attagccagc ctgatcttag actttttatg catgagagtg tgttcatgtg
     1141 tgtgtgttgt gtgtgcgtgg gggagagggg gatgttttgc tgttgttcca cagcaattaa
     1201 acccatctaa tttcaggaag aacaaacatt ctttggtggc tgggggaaag cttcggggcg
     1261 cagaaacttc aggaaaaata aagtccttgg tggcaccaag tcttgtctga tgatgtttgc
     1321 taattatgca gccaggcact tcctgggaaa acacaaaaaa gtaataggca agggtctgcg
     1381 cctgaagctt atcaagaaac aagcaggcaa ctctatctgg atctggcctt ttgtatcttg
     1441 tacagaaaag aggaaagagg acggaaaaag aaaaagagca agaaaacaga aaatctaaag
     1501 gtcaatttcc agtgaaggac tctgtctcag atgtaattaa ccttttgatt taggacgctc
     1561 tgtctcgtaa ataagctggc aattagttta tttggctatc atgtgatagt aatgactctc
     1621 attagcatga tgtgacctcc tctaatctgt tgcctcccag actctttact ggatttcttg
     1681 tcttgcctgg tgaaccggcc tctgacggct gctgatgtgc tccctctgat agtacacacg
     1741 agtctaatgt aaaaggaaga gttgc
//
LOCUS       KCVB01000786            1565 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37395pri ultra-conserved element locus
            uce-844, sequence.
ACCESSION   KCVB01000786
VERSION     KCVB01000786.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37395pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1565
                     /note="ultra-conserved element locus uce-844"
BASE COUNT          451 a          376 c          286 g          452 t
ORIGIN      
        1 tataaaaaag cacagctatt aaacatggga ttttgaaaat taagtagaat atcttattcc
       61 cgtattgagt ttggagtgaa actttttgtg cgcgtgtgtg atggtgttgc catttggaat
      121 atgagacagt gcttgtgctt tgtagaatat cttgctttac ctgtctcaca tatctgttat
      181 ttttcgagga ccaatgtaag gaacagagta ggtggtacat tgtgttacct gtctctccat
      241 gtctgtcttt gctggtgtct gtctgtcaag gctgacagca gggtttctgc catctccctc
      301 cttccttcct tcacttggcc ctcctcctcc acggtagcca gaaacagcat cgtctgaaac
      361 agagaagcag gagatgaaaa tatgtgcaat aagtagaaat aaatgtaaag atgtgaaaca
      421 gcagattgac ttcaaccact ggaaaaacgt aattccgaaa aaaacacaaa caaaaaaacc
      481 tctcaaacta tcttcattaa ttatgacttc tattttacaa cgcaaaacaa tactgtcttc
      541 tacaaagagt ggctaaaata atattggtca atttaccacg cactacaatg agtaagacat
      601 tttaaagctt acccaattca aatcttctct taaacagtaa acagtgtgga aagagtgaga
      661 agactacctg aagcaattcc tcccatcctt gagcttctat cctcagttta gaaattactc
      721 agctctgagc caagactcca ttataacatc cctgggacgg cgacatacag taagtctagt
      781 ataaagtcag tttcacatca gaggatgggc aaattacacc caatattaga gtgtgagtca
      841 attaatcacg tcctgcacag cacagtgtaa tgtgtgagta tccccctgtt gaacatgaat
      901 tacaggccgg cttaaagtgt gacgtggcac atcagtgcta atctgagaca tcctggcaaa
      961 ccttcgcctc caagtcctcc gcagcctcta agctggttga gaaggagtca aatgaaaaag
     1021 gatctggcag cccaaattaa atgcagtgct gtcagccaca ctcaaatccc tctttctcct
     1081 tcttcttctc tacccttcac agactctctc cctctctgaa tatccttata tgcccccctc
     1141 ctccttttcc cagcctctga gaagtgatgg atctgtaact ctgactgact ctgacgcctt
     1201 gttaagaaca gtctcttcct gtcacatcca ccagtaaagc gcgttcattt cttcccatta
     1261 acagtcactc gcctccccag tcatccaacc ctcttttcaa aaaacccaca atgtaaggga
     1321 accttcattt cttctcctct cccctttttt ttaatccttt tctgacaatc cacctgtctg
     1381 tcttacttca tgtaaagata ggtttgattg tctgtgtgtg tatttccaaa ggaaaggagt
     1441 gtgatttgca gacagaggaa ttgcttactt cctccacacc acacaggaca ggtgtcaaaa
     1501 catgtggcgg tgtgtgactg attacacctg actcaaagaa gacaccaaag attgcgatga
     1561 ccatg
//
LOCUS       KCVB01000787            1714 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37430pri ultra-conserved element locus
            uce-219, sequence.
ACCESSION   KCVB01000787
VERSION     KCVB01000787.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37430pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1714
                     /note="ultra-conserved element locus uce-219"
BASE COUNT          489 a          344 c          308 g          573 t
ORIGIN      
        1 gtgctctttt cttaatgtac caatttttta ccttaatctc tgctgtctgc agtgttcagc
       61 cctgactgtc ctgtgtgatc acagacatga ctgaactaac agcagccaat cgactcgctc
      121 atggactcaa atgtcttgtc gagtcgtgac ttacagatgt tgggactttg aacgagcttt
      181 tcttacttcc actttggctc tctatatgca cattttttat ttgttttcta ttgtttgtga
      241 ttgtgtttat agaatacttt tagtattagc cagtacaaga gtatgattgt gtttttctgt
      301 gtatgtggta ggatgtgatc ttaacactat catatctttt acaatgtaca aacaaatgta
      361 taatttgcag ttatttacaa gttttgatat tttggatatt gtttgtcatt cccagttatt
      421 tagaactaag tcaaaatatg gtttgtgttg tttaattttg tcttgttttt tggtaatcaa
      481 ttgatcccaa atctttatgt ttcttcttgt tacaatcaca cacctcaatt tatttctcca
      541 gaaaaataca cacatctcag aggtgaaacc gctgtaacag actcttctct tgcagatact
      601 tccctcagtt gatgagcaat gaaactgatt tgtgttcaca ttatttattt ttgctaatta
      661 gctcgatgca gacacatgat tgacttactc catcttatga ctgaagctgc tgaaagcgat
      721 tagtgtgtca gtctccactc ataaagcagc caaaagttca acaatataca ttctctgggc
      781 ccttttatca ctcccaacac aatgttttgc agctgcacgt cagggttcaa gaaccaacgt
      841 tgtgatgagc tggccaaccc ctgcgattac ttctgtcaga atgaggggat gtgcacatta
      901 acagctctta acaaaccacg ctgcaagtag gtccttctca cctctccagc tgtattcaga
      961 gcccactcac cccctttgtc tacaccccca cctcaactcc atctggctcc atttcagcca
     1021 cagtccagta tatgggctgt ttgtcacagt gccgccttgt agcgaccgat catgtgataa
     1081 gtgccggata aagtaatgac ttatttaata gaatcaacaa actgaaagat gatgtaatga
     1141 catgactttg ctcagggaat cttacttccc tagattagga tcacgtgtga ttctttttgg
     1201 acaggtatgt gtctttattt aaacgtacta caaggaagtt gtaactgcaa gagcaaatga
     1261 tgccatcaga gagaagattc tatatagtta ttcttgatgc ctgtggctgc ctcggattca
     1321 ctatcaaatc aaatgaaacc tttgacagaa gaactgatat taacattttc ccaggcatag
     1381 ttttgaactg acttaagagc acaaaaaaat gataactgac agtgatagag aacagatgaa
     1441 aacatgagtc aacattgttc tgtcatgaaa cggttggtga gaattgcagg atttgaaaaa
     1501 tggagaatgt tgacaagttt agcgcactca acaaaattac tataactgag cagccaaatc
     1561 tgttatttgt aggattacat gtaacttgta tgaacgttac cgttactaaa acaaagttta
     1621 ctttgtttca ccttcatcat gttacagtta ttgatcttat gtagctacaa atagatgcag
     1681 tacgttaact catctctatg cattctgcaa acat
//
LOCUS       KCVB01000788            1426 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37476pri ultra-conserved element locus
            uce-1192, sequence.
ACCESSION   KCVB01000788
VERSION     KCVB01000788.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37476pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1426
                     /note="ultra-conserved element locus uce-1192"
BASE COUNT          409 a          350 c          263 g          404 t
ORIGIN      
        1 atccccctgc ttccccctgc cccaaactca cacacataca aacacacaca caaacacaca
       61 cacagcagct ttccgcagag ccttagagac aatgactcgc ggccccttcc tgtttaagga
      121 tagctgtcag aggatgatgg ctccgattca acactgcagc aggtttgcac agagctcccc
      181 gataaaacgg aaaaaagcaa agcctccact ctctttcctc tctctctccc tctctctctc
      241 tctctgtgtc tctgtctttc tctcgctctc ttcatcactc tccctctccc tccacagttc
      301 aacactgatt tgactgctag cataattttg cagaggagac tatttgcaga tgcagcagag
      361 ttatcaatcc gaacagacac acaatccggg tccaatgtca cttctcctca ttatgttaat
      421 ggttaataca tgcaaatgaa aacattccgg caatttgatt tctttgaaga tagtttgcat
      481 attgtcttag ccataatgat ggtacacttt ctccccagct tcgatttatg caaggctagt
      541 tagttcattt ttatcagctc tgagttcatt tctagggcta ctggaatgtg ttttcccctt
      601 cccctcacta tttaactaat taggattcag gtaccataag cagatttttc acgctattgg
      661 tgtttttctt aattaagaaa caaatgaatt ctccccagca aaatagatgg aacaacggga
      721 tagggtgcca ggggccagat gcggcgcttc ttaattagaa gtcactccat tgtctgaggt
      781 tttttaatga agctccaatc taaatggata tcaaaaaagc ttctagtgaa gaaaaaaaca
      841 cacacacaca caccggccat ttaaaataat aaggaaataa agataatgaa agacaaaaga
      901 tatgtactag cagccccggc tcacacctcc agttcagtgc gtgcagtgct ctcgtcacct
      961 ttggatgagc cctattaaaa cagctcaatc ccttgcctgc ccccgcctgc ctgcagccat
     1021 gtctaatgtt ctgccaagcc aacagtgaaa cactctcttt tcaacttctc atcaaaggtg
     1081 gaagtttcat taccccgtgg atggttatca actaactatt actgaaaact gttgcagctg
     1141 tcaacttcct gtcttgcctt gaaaaacagt tgaggccagc tgaatccatg cttatcatat
     1201 cagtgtcgtt tgtgataggc cgatgttttg tgcatggaag gccgacgcaa aatttccaat
     1261 tagctggaac aaattgtcac ctcaacacaa aatggaagat aaaaaatggt ggtttgcaat
     1321 tagatttgag cacctggaat gtatgtacgg tgtaaaacta aagaaactgt gcagatggaa
     1381 caaaaggaac gttttttgtt ttgaagttaa tttgcaggag ggttct
//
LOCUS       KCVB01000789            1454 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37552pri ultra-conserved element locus
            uce-571, sequence.
ACCESSION   KCVB01000789
VERSION     KCVB01000789.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37552pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1454
                     /note="ultra-conserved element locus uce-571"
BASE COUNT          378 a          370 c          245 g          461 t
ORIGIN      
        1 tagccagtta ttgagcttaa gacaacaaga ggacaactgt tagtgacgtc atttatattt
       61 agtattttaa attcttctga gattagtttg tgtgatcacc tgttacatca tatcatgcag
      121 tgggtagcca atcctccagt gaagtttaag tccaaccttg ctgttggctt ctactgagca
      181 cagaccgtca aaatctattg ttgctatgcc aacacttgtg gtatccaagg caataaacta
      241 cagtatctat agtagtaaac catctgagcc gcctcaacaa agacacaatg cagcactctg
      301 cagcacggta caatatattt ctcgacccca accaagaaaa ttggcttcac agcagttgca
      361 aaaagacaga aaagtgacta cccacaccta tgaatcacct tctcccgcct ccctcctgtc
      421 ttcaaacttc tcctcacctt tctgttttct ctctcatctt ttaacctcat ctcctcacat
      481 ctggttttct cccttcctct cctctcctgt ctcctctcat ctcttctctc ttgtcttcct
      541 ccctccacct ccatcactgc ttgctccttc ctcctatctc tctcttttac ttcctcctgc
      601 atgctctcca ccgcctctct tcctccaacc acctcctctt tttctctcct gccaagtcaa
      661 accaggtcat caggcctttg aggacctgaa agtcatttaa ctcctggtag ataataagta
      721 ttagaatata gctcctcgca gataataagc tttatcactt gattgctctg cacgctccct
      781 actgtctttg gattcatgtc cagggaccta ctcaagcgaa tcacactatt tccttcctgg
      841 caagtcaaat taacacatgg gtgtgagtgt gtgtgtgtgt atcaacgact actcattaca
      901 gggaatgagg gattgctgag ggtgcaaaat attataaaca gcaaaggtga ggctctaata
      961 gtgaacgtgt ggtgaaaaaa gttcaacagc taatgtttaa ttgtccacaa ttgtgacatt
     1021 aacctcttta cttaaccgtc aagctgagtt taattttgat cagctttgat agtcccacgg
     1081 aaacgcttgt ctcttctctt gtcctttgtc ctttcctgcc tgttacttta attttgacct
     1141 actcttctct gctccacctc ttcttcctct catatctcct ctgttccccc ctcaccacta
     1201 aacttctatt ataaaaaaaa gaccactgct ttatcagcta ttattattaa cctgttgata
     1261 tgagatgaaa tgatgaagtg gacaagagga acttcaaggg gaagaactta aataaattga
     1321 ccagaaaaga tcctgatgat gatgatgtca accctgcagg ggaggttttt ggaggtcttg
     1381 ctgacttatg caggtgtaaa gagaattaca gtaagatgta atagagtgtc gcagggatta
     1441 cacatgtttg tagg
//
LOCUS       KCVB01000790            1563 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37613pri ultra-conserved element locus
            uce-1293, sequence.
ACCESSION   KCVB01000790
VERSION     KCVB01000790.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37613pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1563
                     /note="ultra-conserved element locus uce-1293"
BASE COUNT          490 a          302 c          323 g          448 t
ORIGIN      
        1 atttgccttt acaaatacgg tgcatccagt caggtgatcg agcctttagt aaatattgag
       61 tggaggggtg tcccagcagc gtggtctgca ggtttatgtg tagagagagg agtacaaaga
      121 tgagccccca tgaggattca gggagagatg gcttgtgttt gatcacccat caggtgtcag
      181 cgatgtttcg gctgttaaaa acacaggaga ggctgccgct cttgtttgtg ccaagcacag
      241 ctcttcaaag agcctcggaa gctctgtagc tcctcattta aatgggaaag tttctttcag
      301 ctgatagcga gcgctggtgg atctcctgtt gacataggac atttttccct gtgaaaacac
      361 atcactgttg tttttctgcc tcgtggtgag aaggtgatta atgaccaatt ttgagattct
      421 atcaaagcaa ctgcgctgct ctgcaaatcc accctaatta ctacacattg ttaccagcgt
      481 aggagaaaga gaatatcaca tgaatgctga gaccagagga aagaatgact tcttgtcttt
      541 gaaatatata tatttaaaaa tactttaaac aatgctgtat catttctacc tagataatca
      601 cttaatctgt accttcagtg atggttttcc tttttccttt tctacaatcg ctgtagaaaa
      661 agagcaacat aagcttgctc tcagcgcata cttgatcaat tttaattgcc acagttccca
      721 cccctccaat agaaagttga gagaaaaagt aagaaaaaac aaagaatgaa atgtattcct
      781 gcatacaatg atatggtaaa tgttagcgcc tgctgcatcg cactccatat cacttcgctc
      841 catatgaaaa atagtttgct attttggaac cccctggcag ccatagtgga tcaccacaga
      901 cccagagctc atttatcttt tgtcagagat gaagccaaac taacgccatc tcctgaagct
      961 gctaaagaat gaggagggac gtagggaggg taaaaataaa cctggagccc aggtagctac
     1021 acaaagaagt cactctacaa gtaatgggtg gcttctgaaa gtggaactct gtttggaact
     1081 gtctctcctc ttaaaaatta tactatttag tttccgagtt tactcagtta cattcgctgg
     1141 tcagagacgt aatgagaatt tgtaaatgta acagcaggat tatttgacta attaactgat
     1201 tgattaaatt attattttgt tacgtcccat ttagaagtct ctgcaggtat gataaggtta
     1261 agagccaaaa taaaggaagc tcatcataag tgctgtcaga caaactccag agcacctagc
     1321 catcaaatta atcatcctaa aataaataaa tactgaaaaa ataaaattaa acactgatgt
     1381 ataacacatg ctaatttgtt aaatgctacc attaaatgct tcagctatac tgtaatagtt
     1441 ggagcaattg ccacaatacg aggggtgatt gataagattg tggtctacag taaaaggaga
     1501 tgagttacat ggctctcatt acatgcacgt gcagctcaac tctttgagtg attatgtaga
     1561 cag
//
LOCUS       KCVB01000791            1610 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37660pri ultra-conserved element locus
            uce-670, sequence.
ACCESSION   KCVB01000791
VERSION     KCVB01000791.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37660pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1610
                     /note="ultra-conserved element locus uce-670"
BASE COUNT          455 a          384 c          306 g          465 t
ORIGIN      
        1 ctctgacccc tgacccctgt cattcctgtg ctgaactctg acccgcagag ctgtcaatca
       61 tctgcaggcg ccatgagggt gtcactgagc catgcacctg gctggtggtg accagtaaca
      121 aacctcatct cccagtccca cagaggcaga tcaaagactg acactgttac atttttgata
      181 ctcctcctct cgcttagtct cagcaccaaa atgtggagtg actctcagag atgcagctca
      241 tcctctgact accaaaggca ttgcaagcaa ccatttgctt agtccaaact cctgaactgt
      301 aaccaaactg taaccaaacc aatgccacat ccttaaccaa acaaaaacca agctacttcc
      361 aaaactctcc atcagcttca ctgatcacct ttactgatag ctgttatctc tcatacatcc
      421 acgtcatggt gttttaggaa cagcctgcgt cctattagct ggtggctcac ctaaaggaca
      481 cagggagccc aaatcctgat ttgtgattgg acagttctgg ggtgtcacgt gggggggaag
      541 ggactctcaa agtggagctt tgcctgatgg tattatcaag tagacagcca gaatccgatg
      601 gaactaccac ttcagttctc ctctggttaa accttaggaa tgtgtgtaca ttttcagcag
      661 tgactctctg aattgtttct tagtttcagt ttacctgttt acctctcttt atcctttggt
      721 ttttttgttc tgtttcttgt tatttgaaag tgtgacccga ggaagaggcg gggacaggga
      781 ctgggtggtg gctttagaca gacagacaga cagacagccc agccctctga ccctcgaccc
      841 tgggtagcca tgtaatgcca gtcactgccc tttccttcat gctgtataaa aacacacaca
      901 tatatctgtg tatttgtaac ctgaatcttc ttgtgtctgt ccatgcagac aataaaaaaa
      961 ctgtacagta ggtctagttg catgtaatct gtactaatta ttgacaatgg cattataaaa
     1021 tgcaataaaa tacttattac actgtcctac gctgcgtcct tgtttcttct ctggaggtat
     1081 atggcgttgg tttccaactt ccgagacacc caaagaggtc acaaggtaat tctctggggt
     1141 caagagatgg aaggcagaaa aatagataaa tgcaacaaaa aatataaaaa tttttgttta
     1201 tttttctgac ttcacttcaa tctttgcctt tctcctgtgg attaccagat catttttaat
     1261 cctaatactt catacaggcc ttaaagttta ttagtcttct agggaaaaaa aaatactcac
     1321 aactgctaca aataatgcaa tcaatgacaa ggagctgcaa aaaaatttgc ttcgttttaa
     1381 ggggtcataa gcctaaaagg ttggatgcta ctttcccttc tgttagacat attaacacct
     1441 gtacatcaca atgccaccca atacaggagc cctgcaacaa cactaaagcc tcaattttat
     1501 tgagactatg tctgctgtca ttattcagca tgcattatat tctacattcc tgtgccgaca
     1561 cgctgcgccc aacaagacaa tatgtatgat attacacaca atgtgaaaag
//
LOCUS       KCVB01000792            1373 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37700pri ultra-conserved element locus
            uce-44, sequence.
ACCESSION   KCVB01000792
VERSION     KCVB01000792.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37700pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1373
                     /note="ultra-conserved element locus uce-44"
BASE COUNT          413 a          281 c          307 g          372 t
ORIGIN      
        1 aaagtagtat tatcttcttt accagagtgt ggttgttgtg cttcagtgaa aacactctgt
       61 aaagactaac aatcccattg ggttgggctg gaggacggat gtccactact gctgaggtcg
      121 ctgaaacacg caggaaatca ggcgggccta cgccctcagg tggggcaggt cctgttcttc
      181 cactggccca cgcactggca gaacgaccag ctgaattcac cgcccacacc tgttagtcaa
      241 aaccacacat ttgtcaatga tatcatcgta tttcattcac atttctaact gacatgaaat
      301 aataaaaaca gaacatggga cacaaagtga gagccagaca taaacttttt tatcccaaca
      361 ttaggattgc agacacattt tattaactct taatgatgaa agcttccctt aattacccag
      421 catccaccag accaccaaag ggatataagt caattcattg gaccatccat caaaactacc
      481 atcagcaatg agagcaaaac cttttcaaca tgcaacccaa cactaacata tctgcatgac
      541 aattactttt aaccttcatc aaggagttat tctcacacac acgcagacac acacacagac
      601 acacacacac agagtaatct tatttgtgtg cttgtgttgg gtataattaa agggtgtaca
      661 cagcacaggg tcaggcaggg tggagatttg catgtgtaag tcatctgtag cctgtcagaa
      721 gtagtgcaga aggcagatgg atgggtctga gctttgctgt tgaaagtgtg tgtctctgtg
      781 tgtgtgatgt gttgtttgta tctgtgatag ccagttttgt gcgtgtatgt gtgtgcatgc
      841 tagcgtgtta agagttgaat taacctcaat aatagtcaat tagaccgtga tcaatcagac
      901 tgattgatct aagtgacagc acagatggac tgagatggga gatgggaaag aagaatggaa
      961 gatggagaga ctgggaatgg ggaggaggag cagaagaaga agaagggtgg acaaagcatg
     1021 aggggagtga gcaggagacc aaacagagga acctgaacat attttagggt tgtgcctttt
     1081 ttttcatttc atgggtccaa ataagaccaa attggaccca cttgaacctg cgttatcggt
     1141 ttagttctgc agataagatt atattacaag tgtgcttgtg ttttctggtt gcacaactca
     1201 gtgccaactt cttggcatta gtttgtggag acgcacatgc aaacaccttc atcacaaaaa
     1261 cccctttcat gtttgccaaa atgcgcctct ttcactttca tacacatttt gtactgtagc
     1321 gtacacttaa atcaaacaca ttcacttgat tactctactg tgatataata gct
//
LOCUS       KCVB01000793            1352 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37746pri ultra-conserved element locus
            uce-846, sequence.
ACCESSION   KCVB01000793
VERSION     KCVB01000793.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37746pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1352
                     /note="ultra-conserved element locus uce-846"
BASE COUNT          417 a          281 c          294 g          360 t
ORIGIN      
        1 gaaaagcaga ataccatcag tatggactaa ctcaggttca atcacatgca attttgtttt
       61 aaaggtcttt taaatgtatg tacatcatct atgtactcat gtagtcacac cacaagaggt
      121 catttactta tacccacagt tactttccct tgtaaacttc cagtgtattt tgactgtgtt
      181 caatttgcat gttggtttga gtgtcgctca ccttgctgct cctcaaaggc tgcccacagc
      241 aggtggatgg atggtttctt gggcaaatga atggtgcacg ccttattgta gacatgtctc
      301 acaccgtcag tgctgcagcc ctctagatat tttgcatact tcagagtgaa aaagagaaag
      361 cgagttggaa gaagtgcaag agaggacaga ggaaaaagaa cagagaagaa agagaaaggc
      421 aaaagagaca acacatttta aacctaaatt ctcagttgac agaaacagtg aatttcttca
      481 ttgacaagat cgtttttaga aattttctgt gtaaacccat cagcaccttc atggaaccaa
      541 ttagataagt ttctgtaagg tagtgggaga cgaagaagat gcaacgtagt agttggcaaa
      601 tgctgcagtc atatcttgtg tggcaagctg gccatcccct aatacaacag atacagtcac
      661 atatcaacgt tacacgcaaa gagagtgcag gacgattact gtgtcgatgt gacaacatcg
      721 tggatgggga agtgcaggga ggttgcggga gtgagagaga ggttaaaggt cccattacct
      781 tggtccagaa ctcttcgtag agtgcacaag cgatgaggca tcgctcaaag agaacgatca
      841 cgcgctcctg agtcccattt tcaatttcaa agtccaggta ctccttccag ttgttcagct
      901 gggtcttctc taaggctttg acatggaagt atggtctctt aatctgaaaa agaacatgat
      961 taaatgtcaa ataatgataa taatgaaatg ataaaacaca tttctaaaaa tgaacatagt
     1021 tggagaaaat taagacacat accccttcct caaaggccca gcgtttgctg acttcatgtt
     1081 cgttgtggtt gaacacttcc tgccgaacct ctatcacctt gtggcgcata ttctcgattt
     1141 cggtcactct ctacattaaa gatacacaca caaaaaaaac acacatggtc aatgctctca
     1201 tgcacgtgtc accataggac attctccact tgatgtcaaa actgctagtc ctgcagccaa
     1261 ggatttacat caccattagc ttgccagaga caagaagcct gtagagaagg gaggtatgtt
     1321 tgcaccctgc atatcagcct ctggggtcaa tg
//
LOCUS       KCVB01000794            1627 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37791pri ultra-conserved element locus
            uce-728, sequence.
ACCESSION   KCVB01000794
VERSION     KCVB01000794.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37791pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1627
                     /note="ultra-conserved element locus uce-728"
BASE COUNT          514 a          282 c          302 g          529 t
ORIGIN      
        1 caaacaataa atccaatcta gcttcagcaa attaaatttc cctatggccc cttatgcgtc
       61 attttacatt tgaaatggat tgttatcaaa aggcattgca tgtaaatgtc tgcttttttc
      121 tgtttatatt tgttacattt ctgattagat attgttttct ttagtatatt ggaggtgagc
      181 acctcctaag attagaaaga gcttttcaca gagatctgca tgacttgcca gccgcagagc
      241 caagggttaa tcttccaatt gtcgcttcaa tagctgtatg tcacacacat caacatttct
      301 gatgagccat gtatctacag tatatgtgac atactgtcta catgttgtct gaaacacgcc
      361 aaaaaatgat gtgtttgttg aatgagcaca cattgtctat ctctacattg gctttgctaa
      421 aggttggtga aatgtttttg agttatcacg gcacagcgta ctttatattt ctgtacattt
      481 gcaagtcaaa ttgcagtgtt actactttat tgaaaggctt ttgccatatt cttcagcttt
      541 acaactttga aaacttccac agatccagac atttatactt cctgatttct cgcatcatct
      601 ctccaagata gtgataatac gatttctgtg tttttcgctg ttgagaaaca aactggatta
      661 tgaacagatc actacataaa cagctttcta aaaggaggca gctattttaa aaaaggctga
      721 agaaatgcaa agagggattt aaattttaat ttgaatttat tccagcaagt gaaaggttct
      781 tttgagaggg gatttcagca aagatgatgg tatttaaaag tgataaacaa gcatctaaac
      841 ctccagtgga aaacagtcat tttgcttctt tctagtgtag gggaggaatt gggcttgcta
      901 tggtcacatc caaaaaataa gaataagtaa tagtagtaat taaaatgtaa ataagagaga
      961 tccatggaga tatttcaact gatttctgtc ataaattgtg taattaacat tttgtcttga
     1021 cacaccttgt gactctggat gtatagctat atgtcctcag gtgcaaattc ttgttctccg
     1081 tgcacttggc aaagcgtact ttatactgta catggatgct ttaaaaaaat ctttcacact
     1141 tatcaaatgc aatttaaatc ctgtgcctca acactggaga ggaacagcaa gttcaaaatt
     1201 aaatgcctta caaattagga cttgtgttaa ttaaggactg ttgttatgca gtttgagaca
     1261 gtatcaggaa aaaaatcaag tgaatttttg gatgaggaat tcactctggg cagtcacatg
     1321 tcacttgtag ctgacaaagg atgactaacc gaggctgtca tctattatat tcataatata
     1381 tggattataa tgatgtgact ggatttcaaa tgttcctatt ctcttctgga tgaccactga
     1441 acttaagcag agaaacctat gccacacaag aaagacaaag agttacatca ttttgttgaa
     1501 cagttaatcc gatcatggac agtgtttgtg catttgctat cttcggaaaa tgccagcaaa
     1561 ggcgctaagt aaacctattt tacaagactc cctcacaagg atacagtgcc agtatcggga
     1621 taaaatt
//
LOCUS       KCVB01000795            1323 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37861pri ultra-conserved element locus
            uce-1072, sequence.
ACCESSION   KCVB01000795
VERSION     KCVB01000795.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37861pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1323
                     /note="ultra-conserved element locus uce-1072"
BASE COUNT          409 a          264 c          252 g          398 t
ORIGIN      
        1 attctcaagc agcaggtggg tctactttct acatgtgtgc tgacatgtct tttttcctcc
       61 ctttctcctt atctaagtgc tccgacacac aaagtccctg tgttcactgc aggaaatgct
      121 gagtggttat tttgtgatgc tttaaagtct gcttttcaat tgccatcatt acttctggta
      181 ctaaacttat tgttgtgtct ttttgtccaa cacaactgca atagagcggc tggaaagtga
      241 ttttatctca ggatgttttc cgatgtctgt aatattaaga gattatgttg gttttttttt
      301 acaggaaaag atcaagcgta ttcagcagat tcggatggag aaggaactca gggcgcagca
      361 aattttggag gtagagaatc tgatccaaca gcccctctga ttaagcagaa cattcctgga
      421 tagctacagc ccctcagtct atagtgtttt aatcttccta aagctcatta ctgaacagca
      481 gcatgcattg gagggagttg cgtcttctta ggcataattc cacatggaac taatgctaaa
      541 ttggtcttgg ttgacggctg ttgaatatta aaatggctgt tgctgcctct tcagacctta
      601 tttaaattgg acagaaaaag tctttttttc atcagacaac agtaacagtt tagcataagt
      661 gggtcgagga aaaaaaaagg tgctgttttc cacacacgca aacacacatg caaaaatgca
      721 tgaacatcca tgcacacaca cacacacaca cacacacaca cacaattata ctctaggagc
      781 ttcaaagtgt tttagcagga ctaaatcaat tttgagttga cacggttgac aagccactct
      841 taacgggagg cttcaacata ttcacaatta ggtgttaaag agatacatta ttcattcgct
      901 gtgttttaca aatttcactg gcattcagaa gcataaaatc attgctcaaa ccagaaaact
      961 gcaaacaatc agttaaagtg cagcaaagcc ctcacccaag actgattgat acatgtctgt
     1021 ctttgtgatt tcttgacctt aatctttatt cacaggccaa acggaaaaag aagcaagaag
     1081 cggccaatgc caaaatattg gaggctgaaa aacgcataaa ggtatgttta tattttaatt
     1141 caaacccctg aaacagattt aaaattacag ctactaaaat attcatacat gtgtcttttt
     1201 aatcaaaagt cctgtggatt agattatttc atttgtacca tgatgtggag cttgttccac
     1261 ttaagaagca gctggcaata aaattacagt gggcctttga aacgggctga aattccatca
     1321 gtc
//
LOCUS       KCVB01000796            1265 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37899pri ultra-conserved element locus
            uce-341, sequence.
ACCESSION   KCVB01000796
VERSION     KCVB01000796.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37899pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1265
                     /note="ultra-conserved element locus uce-341"
BASE COUNT          360 a          259 c          233 g          413 t
ORIGIN      
        1 taagtcactt taagaaaaga tagggaataa aaaaacggac ttacatcctt ttttactttt
       61 tgttatacta ccattatgga aagcacaaaa aaatttcacc ataatgtgtt atttgctacc
      121 aaacctacac ccatgcttca gttgcatgtc ttatattatc ttccaacttg cccacagttg
      181 tgtgtttaag tgcagggcac cagctcggac atggcagaaa tactccttgt gatgtgttta
      241 agtcaaggcc ggcgtttcag ctgaatgtga catcatctag cacatgtgag aagtgatgaa
      301 agttgctaat gttagtgata tatcctccct ttttggtcct tcccaggtct gttttaagag
      361 cggttacagt gatgacgaga ttgaagagcc ctcccgactt taaaccactc cctccacctg
      421 cctcctcctc ccccctcgcc actgaacttc accacaaact ccaaccaagc gcgaatctta
      481 ggaactcagt gaatgttgat ctgtatttat gagctgtatt aaaagtgtat tataattaaa
      541 gaaaaaagta tgagtttaaa gattttgtac atttgtactt gaatttatgt ctagatgtat
      601 attttcacta aaggttgtat tgtacaaaaa gccataaaag taccacttga atgcatacat
      661 tacattttta tttcctgttc attttctttt ggaatgagta tggattaagt gtattgggga
      721 gtttttttaa gaagcacagt accagtagtc tttttatttt tctcaatgta gcataaggcc
      781 tttttgttta gaaatgtgct gtatgttttt cattgattcg gtcaacgtga cgtgatgtta
      841 acacatcaga cccacccagt cccaaagtaa cccttagcct ctgcacccct caaggctcag
      901 gtatatctga agtgcataga tccaaatgat atggggaaaa aacaaaattt ccagtctaac
      961 gttgctgtgt tgatgccatg ctgcttaagc cttcctatgt ttcccagatc tccacctgcc
     1021 ccctttaatt aaagttggtg tggaagtgaa aaagtccttc cctccgccca gttacgtaga
     1081 ttgccctgta ctggttggca tgtgtagtaa actgtcttct aggtgcctga ttttagcaga
     1141 gatgggatgg atttatttct gacaatcttt tggtcagcat gttcaacaat atgaatcttt
     1201 taaaatacaa aggaaatatt acagaggctt ttcccccata atacaaggcc tgataattac
     1261 cattc
//
LOCUS       KCVB01000797            1279 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37955pri ultra-conserved element locus
            uce-304, sequence.
ACCESSION   KCVB01000797
VERSION     KCVB01000797.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37955pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1279
                     /note="ultra-conserved element locus uce-304"
BASE COUNT          225 a          324 c          243 g          487 t
ORIGIN      
        1 acaggtgtgg ataatcagta acaggaagca gaaattgata caacaggaga agttgcaaca
       61 atgtggcttg ctgatgtgtt ttttggataa taatggagct ttatggcaca gagaaattat
      121 caggctttag ctacagaggc aaattagcat catttattgt agatctctgt cctttcattg
      181 gatttgttga ctataagaaa aatgcactat atgacccgac agttattgtt ttcgctattc
      241 aaaatgacaa aacgacactt aaaaatagtg cgaaacttaa cctctgcaac ataatttatt
      301 agaggaattt aagttgttta aaataaactt atcattgaac ttattgttca tcttcagtgt
      361 tgctcacgag cttgtagggc ctccagtatg gctgccacag ggtgtattac gccacctaca
      421 ggcccattat gatttatttc tctcttttct ctttttcttt ctatctcagt cctcttcttg
      481 ctgctcttct tcctcctcct gctagggttg cttgtcgtag tggatgcctg tctgactgtt
      541 tgtcctttcg tccgtctgtc gtgtccatcc tgtctgtctg tctgtctgtc tgttagtgtt
      601 gtggttgccg ggacagatgc actgatgcct gtgattcgat gctttatctc cactagctcc
      661 agtgtaggtt ggaggggctt aaagatgatc gcaggaggac cttcagctct cgagtaacta
      721 tcccgccccc aaccctttgc ctttccctca ccctcctccg aggagcgcgc aaaacaaacg
      781 ccccgctctg tgttccccct ctccttcctc gcttgcctag ctatctctct agctctcgct
      841 cactctcttt ctctgtgtgt gtctatctgt ctactgccac tggtcttctt tctcgttctc
      901 aatctgtctg tcgttcactc tgtctcactt tttctctgtc cggtctgtta ggattctttc
      961 tttctccgtc tcccacacac acttttctgt ctctcgttgt tcactttctc tgtctgtctc
     1021 tccttctctc cctccatctg tctgtctgtc tctgtttctg tcttctccgg ttaacatgct
     1081 ctgctttcag tagctatgtc tggtgtgctg gatgtctgtt aagtagatgc catctggccg
     1141 ctctagcggt ctttattgtt tttgttcttt tcttcctgtt ttccctcctt tctgctatgt
     1201 ctcggctctg tagtttagtg aaatctgtct agtccgtgct tcctgttttg ttttgtttct
     1261 ttctttcttt ctttctttc
//
LOCUS       KCVB01000798            1848 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 37999pri ultra-conserved element locus
            uce-1336, sequence.
ACCESSION   KCVB01000798
VERSION     KCVB01000798.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="37999pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1848
                     /note="ultra-conserved element locus uce-1336"
BASE COUNT          455 a          454 c          372 g          567 t
ORIGIN      
        1 gcgactgttt cttcagtaga aactatgtgc atggcttgat acctagggca agtggaaagc
       61 tggtgttgtt tcctttttgg cgttatttgg gttaatttac agtgatccaa ttctactgca
      121 ctccagcagc taagtacaac aaactgccct ttaaatgtga tatgactttg ttgtgtgagt
      181 gctgcatttt ctctctgttt tcatctcttt ctttcctcag tccatgtctg gcttttgata
      241 tactgtagct tctctctgcc ctgctttttt ctttaatttc tcccctgtct gcccctctgc
      301 acttcttttt ctgtctcatc attagtggct gtcccatgtt gaggggagag taatctgaac
      361 tacgtgtccc aggggcccat tcctttactt cctgctgtag gaataagtgg cctattgatt
      421 agttatcagc ccgtaattgg gctacgatct ccctcgccct cagatatccc ccagatatat
      481 gacactcctg acagccggag aggtgggtga ggaggcgggg gtggagcggt ggcggggtgt
      541 tcctcctgcc tgcctgccaa cactttactc aacaaaatgt cttcctgacc acatagatgc
      601 caactgctgc ccacgtctct acttcctgca aagggatttt caacatcatg ccagagaaag
      661 attattcaat cttttaaatt atacagccag agagaggtcc agcaggattt tatgctctgt
      721 gttaactatg agcacccatt ttcgccattt tctcatagca ataaactctg aaattgtaat
      781 cagagtttta aaatgaaaat ggtgcggctt accgagaaag aaacttatat aagctcagag
      841 taagtacacc attcgcatat ggataattgc ctgtgaggga tttcaataca aattgaggct
      901 caggaagcgg ataagcaaca ggaagggtgt ctctgctcct gtgtaagcat ccaaatctac
      961 tgtgagtgcg caaagaacca tgactaactc ttactaagtc tgtttgtgcg tttttaggaa
     1021 aagcttaaag atactttcct ctcttcttca tctcactccc tcttactgtc ataacctccc
     1081 ttttccaaat tatgtgtcgc tctctggatc tctttctttt agttcagttt agagtgagaa
     1141 atgcataaac cgctctgcga atgctccttt gacatgaggt gttaattgtg agagtgtttg
     1201 agcgcttgat caaagagcgc acagaggaga tgaagaggag acgtgtcccg ctgggctctt
     1261 cagacagaca caacatgtca aaccctccat ttagcagcca gattaacgca tatcccagcc
     1321 ctccccctgc tccctcgctc ctcccatctc tccctcaaaa ccacatctga taaacatctc
     1381 atttcaacac tcaatgttgc cataatcaaa agtccacccg ttacatgaag cctgttcatc
     1441 atcctgattc cctcatccct cggtccagca acgatcaacc tcttttccct ccattctcct
     1501 cctccatcgc tcttccccag agaagtgaat atcccagtgg aagtgcattt gtctcctggc
     1561 attaatctag cggagatata aaggattgtt gtatgaggct gtagtgtcct gtgggctgta
     1621 ttgtctctgc ttcctcctct ctgacagagt gcagaataac gcagagagat gtgactttag
     1681 tttgcctgct ccctcacaca ctcctctgct tactgagagc tatgggcaaa tgattcttca
     1741 attcctaagg aacagatgag gggattatta cagggaagtt gaaattaata tttacatata
     1801 tctgcaataa acttaaacca caatgcatgc agaggtgttt acatgctt
//
LOCUS       KCVB01000799            1558 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38043pri ultra-conserved element locus
            uce-84, sequence.
ACCESSION   KCVB01000799
VERSION     KCVB01000799.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38043pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1558
                     /note="ultra-conserved element locus uce-84"
BASE COUNT          443 a          349 c          341 g          425 t
ORIGIN      
        1 tatttgactt acattagttt ggtggctaga aacatgtctc taactgaatg ctaatgttgc
       61 tctgtgtctg ctggatgtgc aaatagtgca aataagtgtt tactaacatg tttgccaaat
      121 taactttaca aggtaataag atgtcatttt ttgtgtttaa agcttgtttt gctgtctgca
      181 agtggccaaa agtagttaat tctacattaa gtaaagtagt ttcttaacaa aaaaaggcgc
      241 aatactgaac tttacagaaa acgaatccag tgagaatgtt tgggtcagag catgagctac
      301 cttgaagctg aaaattactt ctgctgtaaa ggtggggctc tacacctcac tgtttgggca
      361 agacaagtct cagcagatca gcggtggcat ctgtgattta aatggaccac atgtccagaa
      421 acttcagcag cctgtgtctg atcaccacat gatgaatgtc tgtttcactg aagccctcaa
      481 atgcatcaag tgcattaaag cccaattcgg gctgcactgc ttgagaggtc aaggcatata
      541 ggaatctgac agatatcatg agcacggctg taactcctat cttttacacc agagccataa
      601 tgtattgtac gagtactttg aaagttaccc caaggctcac tagccgtctg agtcattgac
      661 acaccagtga ctctgactgc acagtcaatt acccatctac ctttcatggg ccaggagtag
      721 gagacaggga atgagttcat ccatgcattc aacctacgcc agccatgcct tcgccagagg
      781 tgaaacccat atgcagacgg gggagacaag aggatattgg gttatttttt tttctatgta
      841 gagaataaag gttaataaca gtcctttcct gtgtcatatt tgacatgacc agtcatcgca
      901 gtaattacaa tcattacact ccaaaggatg tctagttatc cctcttaaac acaggccgca
      961 caaaggaaat caattattca atcagagctc tcccattcat ccctgcctga gttcacttaa
     1021 ttttgggcct gggtgaatct gccgcaaata aaccccacga ccggagaaga aaagacgtac
     1081 aggcccctaa tttgtaccct ttcagacggt caatcacttt tcaaaggaat gcaagcttat
     1141 tctgacaatc aggccgacgt gaatgggaat gggtttcatc tgctacttta cataagttat
     1201 ctagtgataa agagggcaga agaagagagg gtgtgggggg gagttctgtc gacaagtata
     1261 gcctgtggcc ccctcctctg ttactttcag ccccctctct cctttctctc atcccccttt
     1321 cacctcatct ctcacccatc ctccgctcct catcttgccc gtgcccacta ggattggcag
     1381 catgtaattt aagagcatgg gcacaaagac caaatgccaa gcccataatg gaagctggat
     1441 gtgttctgat ttaaatgggc ttctgtgcat agggcccagg gcctgtaatt aagatcattg
     1501 ggacaaagga gagaagacag tctcacaatg caatcgggac ctggttgaag aagaggga
//
LOCUS       KCVB01000800            2193 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38083pri ultra-conserved element locus
            uce-414, sequence.
ACCESSION   KCVB01000800
VERSION     KCVB01000800.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38083pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2193
                     /note="ultra-conserved element locus uce-414"
BASE COUNT          601 a          509 c          466 g          617 t
ORIGIN      
        1 cctaggtgat aaccttttat gtaccagtct gacttcattg aaatgcatag agataaaaat
       61 gtgtcacact tcacagccaa gctaggcagc cactgatgct cgattattca aatgataatt
      121 gacccccacc tgtagcttgc ttatattcag tagtcaatca ctctttccgc tgttacgata
      181 tggagaaaat acgttctgta atttacatta caaaccactg gtgaatgtca agtaccccag
      241 gcgagccatt tgtgtggaga agaatggaaa aaatatatgg ccaattatga tttccaatct
      301 ttttaataat ttaaatgctg ttgatatgtg aattactggc agcatagctc tcagtcctgt
      361 gccctgttta tttcctagca gtgttatttt ctccctaaca aatgacttga tgttaatctt
      421 catatataat tttcatttgt ctgatataat ggcttctagt ggcttgctgt caaaggctga
      481 taaattgaca agcagtatca tggcactaga atcaacaatg tagaagtgtt tgttggccaa
      541 gacaggggct ttttttctac catagcacac tcatggtaat gaatgagtgc atattcatgt
      601 atgcacaaac acacatacat acacacatat gtgaacacat tcaatcatct gatacttttg
      661 tggcgcaagc tttgatcctc atcgtacctg ttcagggtgt ttcctccaaa tcaccaatgg
      721 cttgtcagtt cctcctcagc attactctcg gcattaatgt tggatctttg cgagcgcgag
      781 cgatgctttt taatgttgtc agtttcatct aagaaaacac acgatacaga tacaatttgc
      841 ataaatttcc aatggaaggc gtgaaagcat gctcggcaac tgtgcctgtt tgtttaaagt
      901 cagccaaagc tctataatta gcagtgttta attatggaaa tggctgccga ggagaatttc
      961 aaacacactt cttccctgga tcattagtca caggggcaaa aaaatgaaat gctttcagca
     1021 atgaagggtt catatttcaa gcttatagct gctaaaaggg tgtactcaga aaatcaattt
     1081 tatgaaagtc cgttcagcac taaagggagt tccatgcaac taagcaattt tgaaataatg
     1141 tcggtggggg cctgattcat ggacggtaac aggactttgc tctccttgcc tgggacatta
     1201 tcctctaagc ccaggtcaga ggtcaacctc tggtttaaat caacctctgt ttagcataac
     1261 tcgattctta aagcgctgct ggcaacccca cactgaatag ataagtagtc ctctgcaggc
     1321 agagatcatg cagtgtgcca gatcaaagct tatgaaagct gtctgatgac taaagtggca
     1381 ggggaggatg ttcatataaa ctatgggagg aaacctaacc ttgacctcat gaagtgctag
     1441 ttaccctaaa ccacaacagt gatgtcttaa ccttggccac agtctcacac ataaacagta
     1501 aacacacaac ttctgcatgc cgccaagcac acaggcgccc accacgctat aaatccaatc
     1561 ccagttgctt ctttaatggc cccacgcatt aagcagggtg tcattccctg gtacgaggga
     1621 tatggacgac acatgtgcag gggactgccg cagacgcatg ggaacgtcag gtctcccagg
     1681 ctctgtttta gaaaccacat caccgccatg cggcgcccac acgctctgtg ggaggcccct
     1741 gagctaatag cttgttgtgg aactttggtg tcttttggtg tgtgccctga gctctcccgt
     1801 ggcggtgaga acaacagcag tggggacatt ttggtgggtc gtttaagctg ggaggtcaca
     1861 tataaaaacg gctacagctg cactgggacg gctggcccac gatcctcaca ggggattccc
     1921 attaggcaga ttgattgttt cctctcctct cctcctttga ttcatgtctt ccatctgagc
     1981 tctaatgagc catcggccgt atggcaccac tcagggcatc tgtgcatcgg cccgtgtact
     2041 gtacatggct aataggaagc gcatactgta ctgtaaatgc acacacacac acacatacag
     2101 ttcagaccac agtcacacct gggatgtgta ttgtctagcc atgctgcttt ctgacattca
     2161 cctacctttt cccctcacga aggtttatct aac
//
LOCUS       KCVB01000801            1667 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38122pri ultra-conserved element locus
            uce-886, sequence.
ACCESSION   KCVB01000801
VERSION     KCVB01000801.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38122pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1667
                     /note="ultra-conserved element locus uce-886"
BASE COUNT          465 a          341 c          392 g          469 t
ORIGIN      
        1 ttaatttata atgaaacaaa ggttatcctt gccctgagca gatgtgattt ggaagtccag
       61 ccccatataa ttcagttttg cgtgaccctg ggctatgact ttgtctgttg gacgttgtgt
      121 gtggtggcat gcggttgtct aaccccaaaa caatgtgtga gcgagagggt gctctctaag
      181 tagcttaatg accttgcctt gtcacaagga agacatgtgt tccacgctcc catattggtc
      241 cgagggcaag actgttagag tcagactgga ctctggttta tgtgtccatt taaaggtctg
      301 catctgctgt ctggccctcc acctcctcgc caagcggctg tctttaggtg tgagaggaag
      361 cagcacacat ttaaagctcc atttcaggga tcacattatg gatgaacggg acagattaac
      421 tgtccaagct aaatcgtggt tctcttcagt cttaggtaag gtagacaaga gagtcaatat
      481 ttatttttat atcccaatta tccagtgtgt tttcaggctg agttggctcg atgaacccac
      541 tgattcccag ctatgtctcc ccacaagccc cttccttgtg tcttcttctg ggctgtgata
      601 ttcccagttc actcattttc gatctcagga ctctttcttg ctctctgatt acacacacgc
      661 tcacacactc agacagacat acacgcacac aggctcacag ccaaatggct gttcattaaa
      721 gacagggacc tgaggggaag caatcgtctg agagacggaa attgcgtgtc acattagatt
      781 agcagtgtaa agcctctgga ttggctcggg ggaataagga attatttcaa ttacattatt
      841 tcaaaaagtc tttaagcaat accaataccg ataaaaacaa attaagagtg atttgggctg
      901 atttgtggcg ctctgagctg tgctttctga caatgtgctg gaaatcagat atgaataatg
      961 tagatattac gctgtctgta atttgttgga gttgaaagca gattatctgt gacaaataat
     1021 accattggtt tgcattgtgt ctcatcaaaa gggaatgttc ccaagtgtca atcacagcag
     1081 ctggagactg tacaggctca gtgagccgtg gaggaaagga taactccatc acctgccatt
     1141 cacagtaaca aagcagtgac accaacacac acccagtgaa agtgatgact tgagttaact
     1201 tgtgtaaatc taatgcactg aatttaagct ttttatggtg aaaagtcttc atgtagagca
     1261 ggaatgtccc tgtttggtgg atatgatcct ccaaatttca atgtatttac aggtgatata
     1321 ggttttcatc aagaatccag atgtttttct tctgtatttt attagagtta ttttgtcaga
     1381 cacctcagtg gcgcagagtt gtcttgtttt tttcctgaaa acttaaaatt gtatttttct
     1441 tttgactcaa tttcactcca acacatccag caaaggcaag gaattgaata tgtccatctc
     1501 aggggaaaga gtacagggag gaagggggga agaggctaca gcacagaaaa agagggcagg
     1561 ggaaaagggg aagaggaggg gagggggatt gtcacacgga gcgcatgaag gagaaaccaa
     1621 cagagtcaga ccgacccgca gccagtccac tcactgaagc cctgatc
//
LOCUS       KCVB01000802            1326 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38167pri ultra-conserved element locus
            uce-79, sequence.
ACCESSION   KCVB01000802
VERSION     KCVB01000802.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38167pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1326
                     /note="ultra-conserved element locus uce-79"
BASE COUNT          426 a          264 c          252 g          384 t
ORIGIN      
        1 gatgtggctg ctgaattttt taaatgcaca ttttcactaa ttaaccaaaa ctagctgcat
       61 agataattac cactaggagt aaggatgaac atatcatttt tgtaatttgg gtagactgac
      121 tctttaaata aaaaacggag caataaacac agtgtacaaa ttcttctttc catatatgct
      181 tcaggtgttc caaacactgt gaaattaaac taatattgcc acatcaaact ttactaaacc
      241 atacaaacat aagtcttagt tgcctgacca cgacgtgccc aaatccgcac ttttattcct
      301 ctttaatccc ttttacattc tgcaaaggtt tttaacatat gcctgagcat caaagcatgt
      361 ttctcatctg atcccccctt aatgtgacac tgttatgaag tgtagaaata caaaaatgta
      421 aaagagattc ccacagatta ttaaaaatca gcattccact tgattcccct atgtgagacc
      481 catatctcaa cttcaataaa gtagcaggct cctaatgcag gtattggggg aaaacaagat
      541 acagatttgc ataatcagtg tcactataca tttgcgtggt agccggagtg tggcaagatg
      601 acacagctgg ggtgctctgg caagaccagt gggataaagg agatgaatat gcattaaata
      661 tgctggcagt tcactgcccc aaatgtccag ccaagtttgt caataacatt tatgtcaggg
      721 atggtatttc acgcacatta gcaggccctg cagcgctgtc ttcaacactg ccatggtagg
      781 acaggtggga ggagctgttt tcctagtatg caattccttc ttaaagagtc agggctcttt
      841 aaaatccttt atctgcctat gttaaaatag aaagcctcaa gaaatgcaat ggaaaaaaaa
      901 atttaaacag ttacttgaac ttctggttcc tcgtggtgat gatacacaaa gtacaatgcc
      961 tgcagaaggc accagagaag aaacaagccg acatactgta catttatttt cttagtgacc
     1021 ttactgtgtt attatctgat caatatgtaa ttatgaagga atgtttggta ctttttcttt
     1081 atatttaata caaatagtca taagtactca tacattcaca tacacaatcg tcttttcgga
     1141 cttcacatgt agctggatgc ctgtgctgat tcctcccagt ctcatgctga ggcatgtaat
     1201 agacctgctg atcaactaga aggcaacatc tcaggatcac attttgcatg aaaagtgcac
     1261 gcatggatga aggtgcagta ccagcaggag atgataatga cagaagcaaa ggacgaacat
     1321 agtatg
//
LOCUS       KCVB01000803            1350 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38205pri ultra-conserved element locus
            uce-750, sequence.
ACCESSION   KCVB01000803
VERSION     KCVB01000803.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38205pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1350
                     /note="ultra-conserved element locus uce-750"
BASE COUNT          376 a          262 c          304 g          408 t
ORIGIN      
        1 ttgtctttta ctgttaggcg ttgtagctct gtggaagagc ctcgctcctg gttggactcg
       61 aatggtaagc acaaggtcag actggtcacc aagcagagaa tggattgttg ttttttgttt
      121 atttgtttta ccagaagtgt ttcaagcact gtttggaaaa ccatcttttg tatgactagg
      181 catcgagctg tttgttacaa gattttattg gtggtcttca accctggtca tggtgagatt
      241 agttgaacta caccacacga gtgagtgatt ttaagctggt ggtgatgcca cagtgaataa
      301 tggagtactg tctcattaga caagtgaatg gtgccatcta acacaatgac accattctgg
      361 gataatggtg cgatcagctc ttcaagacca gagttgctga atcacagcaa gcatgtagtc
      421 acagcagggg gtgaactgag gggggatgtg gggctaaaca gtatattaac tgtgtgttgt
      481 tctcattgcc actgcagccg ccaatctgtg atggctattg gccagagctg gctggcagat
      541 gatgatgatg gttatgatac atctacaccc tactaccaca gctcagtata tggttggtat
      601 aatgggtttg tatgatgggt aagatttctt tccaccctcc atctcctaca tagggaagag
      661 gagctacgtt caatgggtaa agcgtctaaa gagcttttag taaaatgacc gctattggga
      721 tcttaggcca gggctccaga ctgcgaacat tttgtcgcat tttgagacca tttttgaagg
      781 aagtgcgact taattttaaa accaggagca ccagtccgtc ttgcaaatat gccccctccc
      841 caccctccgt ttttttaggc ttatcatgtg aaacaccaac agcagcgttt ctcttagtaa
      901 cgttttgcca cagcaaagaa aattagtcac aaaacaaaac taaacatttc gcaatcatct
      961 catctccact tccccaatat gtaagcattt gttgtttcgc aatgtatttg tttctgtacc
     1021 cgcgaggccc aatcagccca tctctcacaa agagaggagg agggagagac tgcgcctggt
     1081 cgcaggccag agaggtgacc catgactcat gttcataagt ttataaaatg tagtgtagag
     1141 aaaatgaaat gagagaaaat gactttctct taaagcacta cttaacttat tgagtttttc
     1201 cttaaaagta aaacaacact ttatttctat aaattgtcta tttattacca aagaatttat
     1261 cagtaagaca gttatggtgt aagaaaaaaa agaatgtgtt gcgttgcatg ttaatttaag
     1321 ttgtgctcct atttttatgc tggtgatcct
//
LOCUS       KCVB01000804            2014 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38238pri ultra-conserved element locus
            uce-966, sequence.
ACCESSION   KCVB01000804
VERSION     KCVB01000804.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38238pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>2014
                     /note="ultra-conserved element locus uce-966"
BASE COUNT          654 a          396 c          402 g          562 t
ORIGIN      
        1 aaaaaaaaaa aaaaaaatta tctaggactc cacttgtctt tgagcttcat tagtgctcca
       61 acaccagcac cataatgcaa atacaggcgc aaaaatatgc tgatcatatt ctctctgatt
      121 ctggttatct gtttacataa tgatagaaaa attcacaagt gggaatcatt acagctgcat
      181 agaaagggca aggtaaagaa ataatgcaaa atgtgccgtg ttctgaatgt tactgcagag
      241 attcttaaga aatgttttat ggttattttg gatttttctc cattttaacg cattttgtgg
      301 aattcctgcc aaaaattgta attgcagttt gaatgcactc taagcaatga tctaaataaa
      361 ttatgggagt ataaaaaagt atatatattt atatttatat ttagctgtct tgcaaatgat
      421 agcaactgtg tgccacttca gcttccagac gacaaactct tcgactgagg gaagaatttc
      481 acttggatta tcaaatcaga tctgactgat gtgacacact tgcagtcaaa aatagcatta
      541 atcttcttgc cgaatcaaaa actaacacac aagtattttt catcctaacc ttactgataa
      601 aactaacaaa tagagatgga tttaagggga gaaaagagta gttaatggat gatgaattag
      661 agacgaggag aaacatcata aattctgaca gggcgctgtt gtctgctgga tgataaacag
      721 cactgttgga tggaatctgc ctctctgtgt atctctcaga cgccggctgc cactttatct
      781 cttccatcct gctagattac acctccaggg ttcaggtggg aatcaatcat tgctattatt
      841 acctgaagtc acaagatgca tctatcacca ctaaggttac cataacctca atattaacag
      901 aggaaaagcc cctctaataa acagcagaaa gaatgggctg gctggtggga tttcatcttc
      961 ctttcacttt taataaggca ctcagaggaa taaagattac aggccatatt tagcatgcag
     1021 aacaaatcag gctcttccag gctcttcctc tcctccactt ctcattctgg ttagtcatta
     1081 agactgagga ggaggaggag aggggaggag gggggggtct agtgtggaga ggcgtttcct
     1141 ggttcagcag tcatgctgcc acgctctgtg ttctcctacc gcctgcagct gctgtcaata
     1201 agactcttct aaggcctgaa atgtgtaaac ttagctttag tctcagtcgc ctggttagac
     1261 cactggcact ctcaggcctc tgtagtgcac aaccagtcag atccagtggt ggaagagtgg
     1321 ttaaagtcct ggggacacaa ccctgttaat gttcatcctc ttttataagc caccattaca
     1381 caatggtgtc tctgtaaaac aagatgcaga aagagagtga gcatcagggc aaaaatggct
     1441 aagcttcttt gaggattaat ggaatttagt caagtaggag tctgtgagtg cattagagca
     1501 ctgacatggg gtcatggcac tgccgagtgt tttcacacgc cacccttggc atgaatcatt
     1561 catcgccatt caaattaata tcatcagtcg aatgaaatat cattaaccac cgctaaagtc
     1621 attccataat aatatacctc caattaaact atgatcagag catcaagcct gataatagat
     1681 tcataccaat taagttagaa atatattcaa aggacttttt actttgaaac cggtcttgtt
     1741 attcccctcg acatccaaca acagcactca ttacagtggc tccaaaacta gaaatggaga
     1801 gaccatgtaa atatttaaaa ccctcacaaa tatataaata ttcattccag aatgatttga
     1861 tgcgctaaat aaataatccc ttgttaaata aatgacaccc taaataaaac tttaaaaaca
     1921 ggatctgggg aaatgaagat gagatgaatc agaggggagg attctggaaa gaggggagta
     1981 agagggggaa atgtcaacta tcactccacc ctat
//
LOCUS       KCVB01000805            1459 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38274pri ultra-conserved element locus
            uce-160, sequence.
ACCESSION   KCVB01000805
VERSION     KCVB01000805.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38274pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1459
                     /note="ultra-conserved element locus uce-160"
BASE COUNT          427 a          270 c          382 g          380 t
ORIGIN      
        1 aaatatgaaa tgttaaaggt ttgaagccgc aaactcaaag ctcaaagtat ctcaggtttt
       61 aagttgatcg ctatttatga gttttgatgt tgcctttaag gctgtgagaa agactgctat
      121 caaaccagag ctgcaccggc tctgaagacc ggataaaaga gttcttagca ggaaatctgt
      181 ctaaatttga tgtacagata gtggtcatcc taggtttgta aaaaatttgt aaacatttga
      241 cttttaatac atttgaggtt tgggtttgcg ttgctgcatg tcaggcaaaa aatgctataa
      301 ttcatctccc tattgaagta caagaaatgc tttttttttt tttgttcaca acagattttt
      361 cactacgagt gaatacaaat gcgcgtctgg aagttaaaca gcgcttacaa gttcagagag
      421 gtttctctgt ctgatcatat ctgaagctca tgttacagag aagctgaaag aagccacatg
      481 gactcaaaac ccagtcagtt atgggtccat gtggttgcac catcatgtaa atgttgcacc
      541 agttttgtaa tagtatacag attaatgact gaacaaaaat ataaagccaa tctttgttct
      601 gaggagacat gtaggagtta aagtccttaa gttgtatata cattatgtat atttatacac
      661 ttaattattt tttacttttc agtaaaaggg gctccacaaa atatgacaat atgccatact
      721 taatgaagta agtcacagac ttattttaga acggaggaac acttccactc ccatctgaaa
      781 gacaaaaaaa tggaaaagag agaaagagga agttagtggt tgattttctg aaaggttttt
      841 ttttgtctca ttttagaaaa ctaaagtaaa aaagaccaaa gagaaagatt cttacctttg
      901 tggaagcact gtggcgatgg atgacattgg agttgataca actcaggttg ttgccctaag
      961 ccaggggcga tcgaggcacc tgccccaaac atgggttcca ggacggccag ttcctccagg
     1021 agggactgag tgcaagacac agcgggcgta ggcgaggcga tgggcgtgga ggtggcgggg
     1081 ctgggggagg cagtgaggat gaggggaggg gaagctggga tggaggagca agaggacaca
     1141 ggagatgaca ccacctcgat ctccattgct tcctccgcta gcccctcccc ttccaccttt
     1201 gccccggtct cagcagccac agtcgccccg catccagcca aggagttaca ttgcgccgat
     1261 tggacgctac agaagtggcc cactccctca aactgggagg gttggcgcca gcacactggg
     1321 gtttgaggag aggttgggct ggagggattg ggcataggag atgaggaggg ggagggggag
     1381 cagaggctgg tgggctgagg aggagcaaag tcatcttgga ggagggtttc taggatgctc
     1441 tcttcaaaca gagaatcat
//
LOCUS       KCVB01000806            1475 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38315pri ultra-conserved element locus
            uce-923, sequence.
ACCESSION   KCVB01000806
VERSION     KCVB01000806.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38315pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1475
                     /note="ultra-conserved element locus uce-923"
BASE COUNT          426 a          342 c          283 g          424 t
ORIGIN      
        1 ggtgaccgac cctctccaac aggcttatgc tggcgtccag cactacgcag gtgatccgtc
       61 tctcctatct cctccactct ctcactccct ccatctgtcc ctttccctct tccctctccg
      121 tttctgtttt tcacatcagt agacactatg acaggtagag atagccggac cagccaattt
      181 cctctcggat taggctactt tacattagga ctgcaaaggg attttaagtt gacttgatta
      241 tgcacccaca cacatacaca cacacacaca caataatctt aattccaaaa gtacaccgag
      301 agctgaggat ctgaacctgc catagccttc tacatgagag atgacataga cctctcatga
      361 ccactgcttt ctgcctgaca tcagctctca tgaacacggt gtaagtctcc ctctctttct
      421 ttgtctgctt agttgaactg cactccgctc cccttttgcc atactctttg tgtagtgatc
      481 cacagtcaaa tgagcccagg ctcccagtca taatgcagtt tatttatgca atgtctagag
      541 gacaaaatga acaatgggat aatgatttga gttttgttgt gtcaaatatt aaaatggaga
      601 tgtgaaaaat tccactctgg ttctcccttt gcaacagcag cctaccccgc cgcctatgcg
      661 ccaatcagcc aagcgttccc ccagcaacca accatcattc cccagcaaca gagggaaggt
      721 aagagcgagc aagcgcgccc acccaagata aaaggtgata attgccatgt tccattctaa
      781 tgtagctgcg ctgctttgaa ttagcgaggg agggaaaaag ctggctgggg aaaatgtgag
      841 aaaaaggctc cccatttaaa gagaagaaca ttagcagtga gatggaaagt tactctgtca
      901 ttattcaaaa actgcactaa ttcaaactcg cagcgttgga ctgcttaatc agtccattac
      961 aatgtgttta agtggttata aatattcatc agattaatta gctgcatgca taatcattta
     1021 ttaccattcc tggtgtaggg ttcgttctta tgccacagca tcagatgtaa taaagcatag
     1081 gatacaccat gatggtaaat cactcttttt gacgccattc gtcagtctca attattcatc
     1141 tatatgattg tatgagacag cgtagagtgg catgaatatt caatatagga actctgaaat
     1201 tgagagtttc caacattgcc tagaggaaaa gttagtttat gtttgcattt ttacagtagt
     1261 ttgagtgtat gtcatggtca cagcgcagaa ctgtatggat aaagtacgga taaacacaga
     1321 cttatcctct catgtcagaa gcttatttgt tttacatgca ggcataattt tactgcaaag
     1381 aaaaacacac agttgttaag gacatcccat ctttcactgt tagcttggta cattttaatc
     1441 attagcaaga attatagaag tcatggtaat gcata
//
LOCUS       KCVB01000807            1768 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38356pri ultra-conserved element locus
            uce-1152, sequence.
ACCESSION   KCVB01000807
VERSION     KCVB01000807.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38356pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1768
                     /note="ultra-conserved element locus uce-1152"
BASE COUNT          482 a          370 c          447 g          469 t
ORIGIN      
        1 cacaggcttt cccagcgtat aagtggggaa atataaagaa gcggtctgaa ataaattaat
       61 gtgtggcctg cacatacctt ctctcaccat tccgacgaca aggcacttct ggaaacggca
      121 gaactggcaa cgatttcttc ggcgtttgtc aacaggacag tttttatttg ctaaacacac
      181 gtattttgca tttttctgaa cggtgcgctg caaagacaga aaaaacataa tgtcactctg
      241 caggcctgtt ttcatggctc tataaacaca taagacacgg actgtggatt gctgcacaag
      301 agatagaaat tttaaaaaaa ggaaaagatg aaaacgatat aatatctgtc atgtcatgct
      361 caggactgcg ctgcatggag agggaataga ccgtcagcaa atacacattc gtggtctttc
      421 atattttact tactcctttg agatcaactg tgtgtgggta gatatgaagt tatttcacac
      481 acgcatttta aggtgttgct tgataatatg aatctcgcat taaaatgtga ttaataaatg
      541 tttaggctgt gttattattg tttttgtttt aaaatactgg gtggatttta actgtcaata
      601 agaaggaaca cactgcatat ctttcatgta attacagtta gactgatgca tggttgctgt
      661 catttcagcc caccttgaaa aatcctttgc agccctcgca ggttctcact ccataatgct
      721 ggcaggctgc gttgtcccca cataccgcac acagaccctc gtttgacgga gatcctcggg
      781 acgggggcga tggcacttgg ctgtccacca gctggtgtcc gtggccgagc tgcagggagt
      841 gaggaaaggc cagaccagcc tgtttccgta tggcgctggg caccgcaaag ctctggccgt
      901 ccaggccgcg gtgcacgccc gggttgtcgt ggttcatgga gacgtgcagc ggcccatcga
      961 accgcatctg acagctggaa acaggagtac cgggcgggga ctgcttgaag gagaagagcg
     1021 aaagccttga caccggattc ttgcgctggt ccatcatatg agacgtggcc gcaacatagt
     1081 tctggtggaa actgtggagg gagccagggt cctcccacat gtgattaggc tgaacctgga
     1141 agtttggcgt actcggggca tgaggcgagg agggtttgaa gtacataggc ccagagtgag
     1201 ccatcatttc ttccgactgg ggaggcaggt ggctctgctg atggtagtta tgcatctgga
     1261 cgtcctccac cttgatagag gactgctctc cagagtgggg catctgatac agacagggcg
     1321 gtttaacgtc gtaactggtg ttatagttgt ccatgaatgt actgaaactt gggagagaag
     1381 tagtagctgt gatctcagtg ttggtcaagt ccatgctaaa cttaacaaac tcaggtgtta
     1441 agaagtcgca gctgtattct cctgcggtgt ggtagctgta gctctgggaa gcaggactag
     1501 ctccttgagg tgatgatcca tactgagcct gaacgcaggg catggctgga aagcaaacgg
     1561 ataaaaacac atcagagttt ctcaaataaa tgttcaaacc gtggaaaagt tttacagttc
     1621 agttcatcta attttactct tagcaagaaa gataaaaagc gctgagcatt aataagtaat
     1681 ttcagaataa aataacatca aactataaaa catctgtgcc tttatttgta aatacttttt
     1741 catatcatac ctcaagtctt ctgacagt
//
LOCUS       KCVB01000808            1524 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38400pri ultra-conserved element locus
            uce-1117, sequence.
ACCESSION   KCVB01000808
VERSION     KCVB01000808.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38400pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1524
                     /note="ultra-conserved element locus uce-1117"
BASE COUNT          392 a          312 c          380 g          440 t
ORIGIN      
        1 tagggagaca aaataaaaaa tttgacaggc aagtagacaa agaaggggca caaagagtta
       61 attaagagaa cagcttgttt gctgtctgtt ctgtgctctg agtgcatgtc tagtaaattg
      121 aaaagaacgt gtgttttgtt gttgtccaca tgtgctttca catttgtaca aatgtgtgtg
      181 tttgtgtaag ttcatcctga ctgcgctgat tgggtcacat ggtttgcaga tgtgatggat
      241 ggcaggctgt aattcccagt ccaagctgga acacaccttc ccccgcactt ctgcagttta
      301 actgcagtgg tcaagaagga tagtgtgggg aggagtgggg gggggagtgg ggggtggggg
      361 gctgctagag agggatgatg gagtgaatga gagataagag gaacgggcca gcatgagcga
      421 gcgaggaaaa aattagatac ctgcctgcct catttgcgaa catatcatta ttcttatttt
      481 aggatttgtt aggctgccag ccgctcctcg agcacaacaa gtggagcgcc tatgtgaatc
      541 gtgcgggcct gagtagattg agagtgcagg ggctgtcaat agatgcatgg cccagtcatc
      601 ttctcaggga agagggggga aaaacaaaca aatgcatctc attattgcag cgcgcgcctc
      661 tgcagtgctc tgggtaaccc atcacagtcc acacagattc aattgcttct tttaaatcag
      721 tttgaagctg cagccacatc tcatctgtct caatagcaga caagagccaa tggacgactc
      781 tgcacacagc aacagctcct cctagtggcc agggattgat tgtaacatgc caaatagatg
      841 gagaggagct gagtgtctgg ctggttctgt tgcttgtggt ccagctgttt ctaccccact
      901 cattagcagt ggatttacct cagatattca gcagcagctc tcttcctgcc cctgctctgc
      961 tctgcagcct tacactcact tttcattctt gccgtatctc tttacgtgtc tgccagagtc
     1021 tttcatgtct ccctcacaca catgtatcca tcctgtactt tccaactccc cactccgtct
     1081 ctgaactcag gcagcggttg cacttgatct ttgatgaaag aaaagcaact ttaacgataa
     1141 ccttttaatt ctatgggctc acccactcgc taaaagctgg cacaataaac agtcaggttt
     1201 gtgtagctcg tgaatgattg aactgattgt cttggtaaag agagatctgt agaaatctct
     1261 ttgatgatta gttggtgaca gatttctcaa tttactgcca aacatttttt tttgtgtccc
     1321 ttgtgttaaa agctacgtgt ttttttctgg aattcctatt tatggtcaga taaaaggatc
     1381 atagagtaga acagtggaga agtgaagacg gctttgatca gtccagaaag cacatattta
     1441 gcttgggggt gatctgagaa gagaagtgtt tggtgtttgc tatgatagat tagtactgga
     1501 tcagtttcat cattatttca tctc
//
LOCUS       KCVB01000809            1608 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38446pri ultra-conserved element locus
            uce-608, sequence.
ACCESSION   KCVB01000809
VERSION     KCVB01000809.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38446pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1608
                     /note="ultra-conserved element locus uce-608"
BASE COUNT          479 a          334 c          309 g          486 t
ORIGIN      
        1 ctgaggaggg gttaaatggt ctaattccac agagattttt ggggggagaa gatacgtgac
       61 agatgcatcg cgataaacat gatgctggcg ttactcagaa agagcaactc agaggccggg
      121 gacaagaatg gcaatgttgt ttcctttgtg atgtgtgcca gtactattta tagggtgtgt
      181 gtgtaaacaa ccagaagtcc cgccttatga ctatcataat tgttaggttt atttatttat
      241 ttgtttaaga taacaaccca gatctcagtc attcaaatga ttgaacgatc tgaggcaaag
      301 atgtgtttgt tttaggtgta tttgatttct tttattgatt tatctatttt caatcataat
      361 catttgaaaa catttttatt ttaattgtat tacttgaagt gaaataacag tttgaaagtg
      421 ttttgaaatg acatcttcat ttgaggattt aagtcacaag aattataatt ccaagggggg
      481 aaaaaatatt tttccgacat gcaaaatggt gaaaaggcat tttattttta aatttttttt
      541 caggaatgca actcctttct gtggaaaaca gcagacaaat atccacatct tcctcatgtc
      601 aaaatctgtc agagcattcg gatccctttg attttttttt ccccctcttg ctcatattga
      661 gacggcttag agaagatcct gaaaagacct tttgaaatgg accagagctg gcactctggc
      721 tatttggtaa ccccctcctt ttcctcagcc tgttttacaa tctcattaaa tcggcccttt
      781 gttttgccac tttccaagct ctgatctctg gcttaggacg agtcaagagg ccgagacagt
      841 ttgaaaaagg ctccctcctc cacaaagagg ccgcttgttc gcaatcattc ccctcttcaa
      901 caccagccag acaaaaaaga gagggagaga gcaacagggt cattcactgg cgcgttatcg
      961 tgagaactta atgaaagttt tataactctt gaaatgttta tgaccttccc actcaacagc
     1021 ttgtcccacc agttttggct gaatctgaca agagtccaca ggctcagcag gcaaaacaag
     1081 aagaaaagca tataggagag aatattatat cttgaaccta aaatatcacc agagaaatgt
     1141 gaggcttctc ccactttagg tgagactatc accacctggc ctagttttta ctcccagata
     1201 gtgtacatgc catcggtact tgaccgtcta gatgctacaa ggagtgactg cacatgatat
     1261 tataatctct ttggcagccc agccctctca ggcacttcta acagaatctt cctcttcttc
     1321 cctgcccagc agctttccaa ctcttggtaa atactgctct actatggcct tattatgcaa
     1381 cattacattc tattatgtca caggatatac aaaaatatgg aaaattgaag gggccacaat
     1441 caaaaagaga ttctgcttct gctactgcat cacgttaaga tccaaataac gcattcaaaa
     1501 gtgttgcatc aaaaaccaaa atgaacgctg aaagcagcct tcttcactca atagacagta
     1561 gttactgaat tactcctctt gtgaggactt agcagagatc catcttgg
//
LOCUS       KCVB01000810             979 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38483pri ultra-conserved element locus
            uce-224, sequence.
ACCESSION   KCVB01000810
VERSION     KCVB01000810.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 979)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38483pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>979
                     /note="ultra-conserved element locus uce-224"
BASE COUNT          310 a          220 c          207 g          242 t
ORIGIN      
        1 tgactggcta tgaaatctga gctgctcagg tgcaaaaaaa aagtagtttc caccaaaatc
       61 agaaactcag aatagctgag acccaacgtg tggtggctgc tgctggttat tattgttcca
      121 gcctggttct cggccaggag gacaagtgga ggagtgtcct tcaactgagt ggtcagtgag
      181 gccagaccgg agcccaacac atgcaggcag accaggcaga tggccagagc tattagcccc
      241 aattagaacc aggaaccctc cacgcatctc ccgagctcgc ccatacggac aaacagcagc
      301 gctgcgctca aagggcagac ggacgtgaag ggaaggagag gacgaacaga caacagaggc
      361 cgaagaagaa gaaaaaaggg gctaaaattt aactggacgt ggattcaagg atacaacatg
      421 ttaaacatgt aaacgtgact gcatgtcgca tcctacactg ttgtctctac acaagccaca
      481 ttacaacagt ttacatttca ctcttcatgt cattgaagat cagagtgtcg actacaacaa
      541 tctataaagg agagaccagg cctttaatta gtccgattat aaaagatagt caattaaatt
      601 ttaaatagga acaagaattc aacttcactg aagaaaagaa tattcacagt gtttagagaa
      661 acatagttta attatatcaa agtgtaaagt aaagagagag aatagcttat aaatctaagg
      721 ctgcagtttg tctttttgtt tttttgtttt gaaaattagg ctctaaccag agggattggc
      781 aatgcatgca gaattcgatc aaatttgctt tcaaacggac tgaaaaatag agcacattta
      841 ttctattccg cctgtttaga cataaacaag aacagcgttt tctacatttg ctttcctgct
      901 tgatttattg ccatcctcct ttccagcaat caatttctgt gtttcagtgg catccccccc
      961 cccccccccc ccccccccc
//
LOCUS       KCVB01000811            1249 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38526pri ultra-conserved element locus
            uce-261, sequence.
ACCESSION   KCVB01000811
VERSION     KCVB01000811.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38526pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1249
                     /note="ultra-conserved element locus uce-261"
BASE COUNT          386 a          230 c          276 g          357 t
ORIGIN      
        1 gacttcaaag tagggattgt tgtagataaa ttttctttca atcaataaac caattaacta
       61 ctagtcattt tagccctaat ttagtagttt tagagaaagg taaacccact ttaatgcttc
      121 gagaggggaa accaagagtt aatgctagcc tgtacacata atttattgat aatttattga
      181 ttgattataa tgaactagag gttacacatt acagattttt atttaccatc acagcactaa
      241 agtaggaatt ctagttctcg agaatttatc gtccatagtt gtggtcacct gatttaaaag
      301 ccccattgtt caaataacaa aatgcaggtt agatctgagc ctccctccct ctcccactgg
      361 gatgtctgta taaaacccag tcagttctca ggggtagagg ttgacaggag aagacatcgc
      421 cccagtccct gggagattgg cgaacacaac acatatatta atgcattaaa tgtgttgact
      481 gtgttctgcc cagggatgta ggagagagag agagagaaag gttaggggga agaaagaaaa
      541 gctgttgtaa tgtatggtgt tgtcttgtat agcgcgtctg gcagtggggg gtggggttgg
      601 aggagtgtcg gccagattat aaggcctgtg gtgagagatg agggatggcc gacttgtaaa
      661 caagtgacaa gcgtcctgga tgacaattcc taatccatca tagagctgtc agcggtgacc
      721 ggtgaggtga cggacaggac ccaggagagg ggacagctgc tgcacgtcaa tccacatcat
      781 aatttgaaaa gctttttcac atgagctgaa gcagagggag agaggaagag gaaagaactc
      841 atacatccca ctatctgcta taccttctcc tcaatctgcc tccattattt ttcctcctct
      901 ctctttcttc cttgctgttg aggttatgga tgatgttgtg tcctttttcc ttgacacggt
      961 gagctggaaa gccatggcac cctttaactt gtcctgctga tgggattata atcacaaaaa
     1021 gacaaagtgc atcaaatact tgtaaatgtt cacgaggcac attaaaaaaa aaaatcgtta
     1081 aaaccaccta tggatgtgct cagacatttg tccatattaa aacagtgttg ttagaacgca
     1141 gttacatgca caaactttat ttagctttaa cgaaataaag ctaaataaaa attaaactta
     1201 cttcaaactt gaagtaagtt taattttata acttcagaaa agttccatt
//
LOCUS       KCVB01000812            1500 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38609pri ultra-conserved element locus
            uce-1216, sequence.
ACCESSION   KCVB01000812
VERSION     KCVB01000812.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38609pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1500
                     /note="ultra-conserved element locus uce-1216"
BASE COUNT          395 a          328 c          364 g          413 t
ORIGIN      
        1 ttattaagaa cacatctatt gtaagacatg cctaatatac cattcaaggc atattttttg
       61 tgtttataga cccatgatga agtccaagtg ctggacagtg aaaaggtgat aattatttga
      121 tgccatctta gaaattccgt tcatagtcgt aaataatgta tgtgaattct gtcttttgtt
      181 tcctatccag gtatccaacg gctgtgtgag caagatcctg ggcagatatt atgaaactgg
      241 ctccataaga ccgagagcaa taggcggcag caagccccgg gtcgccactc cagaggtggt
      301 cgcgaaaatc gcgcagtaca agcgggagtg tccgtctatc tttgcgtggg agatccgtga
      361 cagacttctg tcggagggga tctgcaccaa cgacaatata cccagtgtaa gtgtattgac
      421 tgtaagttat gtgcatgatt taatttgcca catgaaatga cttttttctc atgcagtgga
      481 ggaaaaggag atgggcaacc aaccattcac tcttattaaa tacactgctt ttcaaagtgt
      541 ggggcacgca ttactgcata tgtgctcagc caaacacaca gtcaattaga aggagcgcgt
      601 tgtccatttg tgcgctttcc tgagcagatt tattatgatt ataaggtgtg tgtgtgtgtg
      661 tatgttgcta tgaacccgct gcacatccac atctttgcgc catgcttgca gaaacacaaa
      721 gatgcagcgc tccatccgat cctaatcttt acttttatca tatgtaattt gtcattcagg
      781 tgtcatcgat aaacagagtc ctccgcaacc tggctagtga aaagcaacag atgggcgcag
      841 atggcatgta tgacaagctg agaatgctca acggtcagac aggaacttgg gggacccggc
      901 ccggctggta ccccggaaca tcagtgccag ggcagcccaa ccaaggtgag cctcattatc
      961 acacagtggc aataaagaca ccttgacgca tgcacggtgg ttaggttgta tcaaagaagg
     1021 atacaaccgg gctgatatgt ctgatttggc ttttcatgct cggtaacatt tatggtcagt
     1081 aacgaaattt ttttttgcct cgcatattgt ctctgttcga agagagatgc attaccgtga
     1141 actccagaga ggtcaccata aattgtcatg tttgaatatg aatagtttta cggcttacta
     1201 ttccagtggt tccggtccac ggctctgttt gctggaaggt attgtattgg catgctttga
     1261 tgccctgaac aagaccccca attaagacta gtcgattaga ggaagccata ggccaagctt
     1321 gcagtctgaa tgcctccact gtcccagaca tggatgtgcg tgacagcggc ttgtcctttc
     1381 agcagaggtg tccatccaac agcctgcatt ttcctcccct cctgatgggg aggcagagcc
     1441 ggggagctgt gtgcggcgat attagtacaa aggaatgttt tccttcacac gcaatgattg
//
LOCUS       KCVB01000813            1787 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38655pri ultra-conserved element locus
            uce-509, sequence.
ACCESSION   KCVB01000813
VERSION     KCVB01000813.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38655pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1787
                     /note="ultra-conserved element locus uce-509"
BASE COUNT          478 a          494 c          425 g          390 t
ORIGIN      
        1 cactaatgta cttgaacaaa ccaaaccagg gctgaactag actgcattta tcacataaac
       61 cgacgtttcg gtcatccaca cgcgccagcg ccggtaaaca gaaagccaca caaacagatc
      121 tgctggtaac tttgtgagta caactttttg gagtcaacag cgataaagag agtgaggggg
      181 cggaaccctg ctgagcccct gcgctgaacg acagccaatc gcttcgttct cctaactgcc
      241 agtcaccgaa atccgtccaa tacccgcagt gccatttcta aactgtattc cccactgtgt
      301 cctccaactg ttgtatgttc tgccaatcgc ttgaggacag agtgggaaaa ggagcaagca
      361 gagttagaag ccggacaaaa gaacttatag accccttata tacatatttt cctaatgttt
      421 tagagagcgt ggtgtggtga aaaacaattg cggtgagtgc ggatggactt gtcagtgtag
      481 ttatggaacc ccctttaagc aagaggaatc cggcgataag attagcggat ttggcagcga
      541 ctcaacccct tcctcatcag aatatgacag gcttcccggg gctagggggg catcaccctc
      601 tctcccacca tgcccacctc caccctgggg agctgggcaa cgaccccgga gtggcactca
      661 ctccatttgg accagagcac atggcacaga caaatgctct caaacttagc ccatctcagc
      721 acattcagag ccatcccgaa gcccagaccg cggcatcttt cacttctgct cagaccacag
      781 ttggtttccc cgtggctcac ccccactcag gctactcaag cagcagggac ttcatcctca
      841 ggagagaact ctcagcctct gctatgcatg cacttggcga ccagcatagt tccgcctcct
      901 cccctcatca ccatggcatg ttcatctccc caacaggtgc ttatgggcac acggaaagtg
      961 gggcccattc acttttcact ggacttcacg accaggcgtc cccaggtgcc caccatgccc
     1021 tcaatgggca gatgcgcctg ggtataccgg gggacatcta cggcaggcca gagcacttcg
     1081 ggcacaggcc agagcactat ggaccctctt ctctccacag ctacaactcg atgaacctca
     1141 atgtgaacat cgcttctgct cctcacggag cggcgggggc gtttttaaga tacatgcggc
     1201 agcccataaa gcaagagcta atctgcaagt ggattgacca ggagcaaaat caaaagaagc
     1261 cctgctccaa aacttacagc accatgcacg aactggtcaa ccacgtcacg gtggagcatg
     1321 tcgggggacc ggagcagagc aaccacgtct gtttttggga ggaatgtcca cgcgaaggaa
     1381 aggcgttcaa agcgaagtac aaactgataa atcacatccg agttcatacg ggagaaaagc
     1441 ccttcccgtg ccctttcccg ggttgtggga aagtcttcgc tcgatcggag aatttaaaga
     1501 ttcacaagag gactcacaca ggtcagtacg tctaattgtg taaaacttgc atgccgcaat
     1561 gtgaaaaaat attgaccttg tctactcaca tcctaaacgg atgcgagcgg gtgcgagcgc
     1621 gcgtaattgc gcacacaagc gagcaaaaaa agttttattc agtgacgcat tcgcacagtg
     1681 agcaaacgtg tttcatttcc tttctttaaa ataaactttt tttttactcg ctgagacgcc
     1741 tgcagtaggc gtgagaggat taaggcatat catttgtaaa acgtgct
//
LOCUS       KCVB01000814            1630 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38697pri ultra-conserved element locus
            uce-731, sequence.
ACCESSION   KCVB01000814
VERSION     KCVB01000814.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38697pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1630
                     /note="ultra-conserved element locus uce-731"
BASE COUNT          455 a          412 c          293 g          470 t
ORIGIN      
        1 tcaaaagaca caatgtttat tttgcttact gtggtattta cagcgagctg tgtagtacgt
       61 tagttaccta ccatttcatc aaagcccatt tcactcagct gtccacagct gtaaaagctc
      121 ctcatgggct ttgtttggta tagattaaca ttaacctttt aaaaagcctc tgaccacaaa
      181 aacgttgcta ccagacccca gtgagatatg ttatggccta agtgcctttt agcatacact
      241 cactgtgttt catacaaaca cacacaggga tacacactcg gactcacaca catgcatttg
      301 tgcacacaaa ccacaaacac acacacacac acacacacaa tgttccctgc tcagagcatt
      361 acagcctttc ctccttttac acttatctgt actcatgtgt ctgcaacgag cgagcctaag
      421 ttaccaaact cgcaaattgc cctgctgtca cgcaggtccc ctttaagcac gccttttagc
      481 ctcctaaagg acaaataaag tccatttaat aactgcacta aacactaata gtctatgtgt
      541 ggttgacatt tgtgtttgga ctaaaagccc ccctcaggcc actggtggta cagaggcagg
      601 ggcgcgcagc tcccctctgt agcactaaca ggtctgccac agctgaaagc acaagacaac
      661 agctcttaag acactctctc acacactgag ctattgatcc gtctaaaaga acacacagtg
      721 gacgccactt gccactctac tttcaaaaca catttctctc tctctctctt actctcaaaa
      781 aaaaataaaa aaagactgga cactctgcat cacagggggt caggccagca ttttgtatgt
      841 gtgcgtatct ataaaggccc attttagaga cacgttatac tgagttttat atgaaatttc
      901 actgtcttac tgcagcactt tagggatgtg cgcgggtaca ttttgaccag ataacgtttt
      961 tattttcatt tcttaatgca caattgcggc accgtaatgt taagcgtgaa tggggccatt
     1021 ctgtacttag agctgccatt ttacagtcac ttctgcggtt ggcttagtga taaacacaac
     1081 ctcagctggt aacaagggac cgcttcactc tgtgcactgt ttttctctgc taagcctgtc
     1141 aagttcggtc tctttcacaa aaaccttcta ccacttttca aaggacttct ttggaatagt
     1201 aaagcatatt ttctctttct ctttctgtct gcatcacatg cacatacaca cacgtctgca
     1261 cacctaaatg gactcacaaa gtaccttatt ggatccagat ttgctctgca ctttttggct
     1321 gtccacacat caataatgct atgaatgtaa tataattagc atttcccctt ttaaaaaaga
     1381 ggtgaaagaa ataggagagc ttgttaggca aatttatggc tttggtttat atgtcagtaa
     1441 gtggtccagt ggtgttgagc catcttattt ctctatcaga agtggaaagg agaggcttat
     1501 tggttcagtg cttacgatgg gagccatcaa ttagtgttct gcctgccctc cagctccctc
     1561 acctccctcc ctccccaaaa caggcccctt gaagctggca cattaggatc acaaaacctg
     1621 ctataaatac
//
LOCUS       KCVB01000815            1716 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38740pri ultra-conserved element locus
            uce-18, sequence.
ACCESSION   KCVB01000815
VERSION     KCVB01000815.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38740pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1716
                     /note="ultra-conserved element locus uce-18"
BASE COUNT          472 a          380 c          350 g          514 t
ORIGIN      
        1 gcccttgagt tcttgctgtt gttctctgcc atgcgccagc gtggggagtt tgggttgctt
       61 tgcacacaaa gtcacaagga gctaaacgcc tccctttagg gtctgcaggg tataaaccaa
      121 cccattgttt tctcttttgt gttgttagga gaagtgagcg aatgcgaact ttacacccac
      181 ctcgtcaagg ctttctataa taaagagcaa ctttatgcat ttacaagtag aaggaaaata
      241 catcaatcta atgaaccaag tctatctgct ctggagagaa tgggagtctc aagtgacgtg
      301 ctgttcaaag gtaaaacatg cactagatgt tctaagcata taatctacgg cagtagagaa
      361 acaacaatat gcaaaacact aaaaagatat tatttaagat tagatatatg tttaatgttc
      421 aacctttttc tcagacgagt cttttgcccc tgcctctctg ttttcaggtc ctgaccttca
      481 gcgagcacag ggctccaaat gagccaggtc agttttgtag tctttttgtg cttggaggag
      541 ccatgaaata ttcaggctta gtgcttccct gagaccaggc tggtggctgt ggctttgtta
      601 gcacgcctcg ccctgcactc ctgtccagtg tccaccctgc accttgaacc atcgtcttca
      661 ggggtgtgaa ctcgggagtc tgctccctcc gaacataatg tccctttttc cgctcatctt
      721 caccgaatgt tttttcctgc accgctcgcc aagaattctc tatgccgtca tgcaattcct
      781 ctcccctcat tgcttgatag tggaatatat gtacggctat ttccagcagg taagggtggg
      841 ggatatagct ctatgtggac tttgtcaatt catgaccaat ggcgaggtcc tgcattatac
      901 actaagctga gcaggagagc aggtttgttt tattcacaac cttgtggatt caaacagatt
      961 gctcagactg gggaattgac acagatacta tcatacaggt ccaagacatg gagagagaac
     1021 aggtaggaat aaactatgta taaaagcact ggtaagcttc agcctcaggc aaagctacat
     1081 gggctacaat gttaccctcc ccaaagccac aatcaattct tggcttcacc gtatccaaat
     1141 gtttattttt gctcggctga aatataatga cacgtcgttc tgaaatacag ttgtaccctt
     1201 ggctcacatt ggtattacag agcgccttgg ttacatttac cttggattgg gatgagttca
     1261 tgctgatcaa atttaaacaa gttctaaaaa ctgagagttt gagtcttcac tgtttattat
     1321 aaattgagaa atgtgatgga ttcacattgt ttcttgtgcc tattttagag caaagaacat
     1381 gcattgcatt tccagataag ttaacaccaa ccaatttcaa caattcaaat cactccagat
     1441 gcaaaatcct gcatgtcaga gctaacaggt gtaacatatt actgctctat ccgtcctcta
     1501 taaaataatg ccccatcaca ttcaaaaata ttatcatctc attcaaattc ataacactga
     1561 tggctttcaa gcatcatcta tataatcctt gaataatttc cccatgcaac tgtcatatct
     1621 ggagtcgtaa ataactgttc cctgacaaaa tccttggtaa aagggcagga tgtatgtgtg
     1681 gatgttgccg cggtgtgtcc gtcctgtgtg atatat
//
LOCUS       KCVB01000816            1567 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38784pri ultra-conserved element locus
            uce-430, sequence.
ACCESSION   KCVB01000816
VERSION     KCVB01000816.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38784pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1567
                     /note="ultra-conserved element locus uce-430"
BASE COUNT          424 a          330 c          338 g          475 t
ORIGIN      
        1 caacactagc ttttattgga tggtagtttc cttctttttg tttttgactg agaggcttcc
       61 ccccaaatat atttcttaaa ttcctcatca aataaaatct aataaaaagc cttctgaaga
      121 atttaaaaac gccgaattta atgttgtttt caagtattat atagttaatc atcttttttc
      181 caatcatgtc tcagcactga tcggtccctc gcactatctg tgggaaacgt gttatctccc
      241 tctgccattc atgtacacat ttattggatt ccaagtccct gggatcaatc ttttggccac
      301 cagaggagca taactaatgg caacattaga atggctttgt gagagggact ggccggatgc
      361 gggttaacca agcaggaaat aatctaggat tttatatcct gcatgaatgg ccataggtta
      421 tgctgagaat aaattaatcc cccccaaaaa tttagcagtg aggttatctt ttgaatgaaa
      481 cagtttcaga ggggtgggcg gtttgtttgg ttgatttttt ttttttaggg ggtggagggg
      541 tgtttgctcc ccgcatgcac acatgtggaa tttcttggca gataatgacc gccatcttaa
      601 tgctcagcca gggtcggagt atctattctg tctctgaccg caggtctaga cggctttcct
      661 ctggaaagcc tttttcgccc cttcctttta aagacccact tctattttcc tgttgtgccc
      721 aaacaaggat tttataaaac gggtgacatc gccacacaag ctgtctgccg aatccccctt
      781 aattaaagtg acttttaaag ccttcctcag acactaataa accccctctc cattaagatc
      841 ctcctaatta ctgtgtgcaa aggaaagggg gacacacaga gacagacagt gtggaggtaa
      901 gcctgctggc atttggcccc ataagctgag cgccacacaa aggaaaatgt cccctgggtt
      961 acccatccta ccagagagat tccttctttt tttccagcct cgctccccct ctcctgtttt
     1021 ctgctttatg aatgaaagta tatgacatgt ggggctgaca gtgtccattc atggagctgg
     1081 tggaatgtgt gaataagggc tttgaatgag gcatgaatca cagtcacctg atgagtcatt
     1141 aagcagtctt ttgacattac cattgtttaa atgtgcatgg tggaagtgag agtgctgtca
     1201 tttacaaaca ctcaagtgag gctgattaga agagcgagat attcaatgta aactgataga
     1261 tgcaggaatc tcagaaagca tcataaagca tttttttctg gtgaaacttc ggtattaaga
     1321 cgcctattaa aaacattttg ggacatttat tttgcataag gctttgcaat agatggtagt
     1381 cccatcatag tcccacagag tggcttatat tgatgtggac aagacaatct catatcgttg
     1441 cacgtcacac tgagccccaa gtacagagtg tgtggacaat cactacagct tcagtggcaa
     1501 aggcctgccc aggaaggtta aatgtgtttg gttcatgtcc tggtctgcag attaatgaca
     1561 ttaacga
//
LOCUS       KCVB01000817            1308 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38823pri ultra-conserved element locus
            uce-1096, sequence.
ACCESSION   KCVB01000817
VERSION     KCVB01000817.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38823pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1308
                     /note="ultra-conserved element locus uce-1096"
BASE COUNT          383 a          250 c          292 g          383 t
ORIGIN      
        1 aaaatcaatc tctcagtttt aagtggactt cccagaattt agacaaacat tgaaaaataa
       61 agtgccatcg gaaaagattt ccaaccttaa tcatgacaaa ttctacagga gtttcatgtt
      121 cctcacctct ttgctgttat agcaggtcaa atggatttgt atggtgctaa cagtatggat
      181 aagatgacag cagtagatta aagtaaaatt agccttttct tattcctatc tcttccatta
      241 tcttgtgttt taagacatca catctttttt tatcaaaact tgcagcacat ttcatagaca
      301 tttttctctg tctgcttcat gtcttaaaga tgcttgaagc actattggaa gcaaccgaat
      361 gtagctgaga agaataaagc atgtgaattg attcctccat cacctggata ggctgttgat
      421 tttggggaga gttatcgctg ttttcctaag tgtcgcgtgg agatttgctg gagggctgag
      481 tgatcgattc agcagcctga agcatcctag tgtgtttata catggcttgt atggcagctt
      541 tcataagaca tttaccccat gatttgcgat gagcttatgt gtgaataatt aatggcaatt
      601 aatccttaat tacagtaatt aggcaagtca caggaaatta ggctgcagct ggagaagccc
      661 gtgcctagga aagggaggac tggggctgag agaaagtgcc atttagtgac agtggagccg
      721 tggtacaatg attgtgtggc aggcctgcac cagtgtgctc ccttgccagc agagataaga
      781 gtgcaggaag acacagatgc tgctggccag cgagcctttc aatggtgagc caggcctgga
      841 aagcaatcac aggcacagag caccgacaca cccaataaaa ccacctatta aagtcgcagc
      901 aaggtgacct ctgacctcaa catcactaca gttttttcta gtttgcttaa aattacatgc
      961 ttaggtggtg aaccacttca acaggttgtg actgatgagg taacagtaat tggttaatgc
     1021 aacacattga actcattttt tctcactttt atacaactag atttaccact tattggggcc
     1081 cctgaggctg cagactttag cacccaatgt gctgttaaca ccaagtttat gaatctcctg
     1141 tggctgaaag gagagacagt ccaagaaata tttatttgta cctctaataa aacaattagc
     1201 ctgcttagaa ttattcaagg aaactacaac aggcagagtt gtttggttgc tgagttcatt
     1261 gtttggtaat agaaggagag attaggtgga tctgttctgg gcaaagag
//
LOCUS       KCVB01000818            1789 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38863pri ultra-conserved element locus
            uce-358, sequence.
ACCESSION   KCVB01000818
VERSION     KCVB01000818.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38863pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1789
                     /note="ultra-conserved element locus uce-358"
BASE COUNT          528 a          368 c          339 g          554 t
ORIGIN      
        1 atatctgagg gtcaatcggg gttaggcagt cttcctcaac aggaaatgag cttttggtgg
       61 gctgtgttcc caaggtaatg agcaatgtgt gtgtgtttaa tcttagctaa tattgttata
      121 ataactggat aaattattaa aaacaagcaa atggtaacat tctaaagacc accagggcaa
      181 cttgaatttg ttgcattatt ttagaacttt caacagttca gaggaacttc tttgcaaatc
      241 attgtacgga aatgggatct cagatagatt gtgttccttt actgcctaaa tgtgagcctt
      301 gtgtaggagg attggatgga tgatgatgcc aaaaactcta gatataatct ctgtttcctt
      361 gggttaaagg tcattgctac aatacttata caaactactg ctcatctggt aaacttcctc
      421 atcacattcc tgcatgcatg tctttccctc ctttaatact ttgcacatac tatattcatt
      481 ttacccccac aagatacccg tcaactttgt tcttttcatc tgctgtaagg tcattattgc
      541 cttgaatatc tgccgtggag cagcagcagt gagggggtat gaaattcatg tcctaatatg
      601 taaatgaagt catgaacgct ccatattgta taaccacatg catctggcat gattgggcag
      661 tgtgggacaa attgtcctca gccaaggcat tttgctactc agcaaaagct aatgttgagg
      721 agtggaaccg tgttcacaat taccatgata aaggtgagcg ggggaagaat aaattgtgaa
      781 attaggaact tctgtgtgca cggcaaaaaa ataagaaata aaagtcctcc tatataaata
      841 caatacagac ttagaacaaa tcacagaaca aatgcatcaa ataaatactt tattcatgtg
      901 gagctaattt cacccggcca ttttgatcac gtcattccct ctggaaaatt gcaattaagt
      961 ttggagagat aaaacatgcc aactactctg catttcaagg cttgattggc ctatcagcta
     1021 gtcagtatgc cgccatggtt ttcatcactg ttaattgatc ctttctttaa ttaatctgga
     1081 aaggaaagcc catatctcta tcctgtctga tcgtcccctc tgtctgtcat aacacaataa
     1141 aggccctatc ctactgctgg taactctgcc ttagcctctc cctctggctc tcggccaaat
     1201 ggactattta tggtgcacct atctgacgac aagcccgctg tacggagttc aatggctggg
     1261 ttcattaaca gtgctctacc cggctacttc taatttgcat gcagtgacac cttgctttta
     1321 atatggatgt gaaggttagc ggtaggccaa gacactttct caactaccac tggtctaaat
     1381 atcgttcctc cagctccttg tccccccact gtcactaacg ccgccacgtt ttccctctga
     1441 ttcaattaaa ccccctcttt ttttgtcatg ctttgttcct ctgctggcat tcgtcatgta
     1501 tgctagcata catgatgtta gcgtacccca ctgccacatg gaataaaacc tttgattcat
     1561 atgagtgaag actgtatcga tttgtttgat tttaagtatt gtgatggata atcagagctt
     1621 tttcagaagt aaaaagctaa aaaactataa tcagtcgtag actataatta ataaatataa
     1681 cggagtagtt aaagtacatg ttttgaaaag tcagacaaag taaaacaaaa agaataagtg
     1741 acttgaaatc atcgtaaatt caagaattat gagtacttct gtctgcaaa
//
LOCUS       KCVB01000819            1608 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38904pri ultra-conserved element locus
            uce-1277, sequence.
ACCESSION   KCVB01000819
VERSION     KCVB01000819.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38904pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1608
                     /note="ultra-conserved element locus uce-1277"
BASE COUNT          473 a          359 c          356 g          420 t
ORIGIN      
        1 gcttcctggt tttacgttac tggccaaaca cgccccagct gtacataaag tggtaactct
       61 caataagacc cgatggaggc agagggagat cagacacccc gtgatgtaac caaacatccc
      121 agttaaaagc aaagtgaaca gaggggatgc cattgatatt gttgcttgct taaacaggtc
      181 agcgttcatt caaggcaagt gtgatattac ataaagtaat atcatacttg tgagcgatgg
      241 ttcaagccct gaggcctcta caaagagaat tcaaagtttg ccactgtcat gaagtacagc
      301 agtagtcttc tgaatatttc ataccctgtt cttttaatct accttatcct taagacgtcc
      361 atatggactt catagaatgt gtcaatctct tgagtgatag catgtaaaac ccacaaaaca
      421 atcccctaaa ggcaatccca tcaagcagca ttcactaaga gatgtatagc cctatgttaa
      481 gcaaatgggc agagaaatgg ttacccactg aatccattat ttcttctctc atatccattg
      541 tcttaaagcc tcccttatgt gcccttagtg cctttaaaga catgttaaga gaaaagagct
      601 ctctgccttg gaatgagcta acttaattat catagcaact ggcagtgctc tttaacatgt
      661 caatagtgct tggcctgcat gcataatttt gctgtgttat tgtggtggcc tctgcgtttc
      721 tcactgacaa aaacccaaac cacaggcacg ggggcctatt gtcatgggcg catccatctg
      781 gatcaaggcc attagaggat atttgcactt cctctgtggc tcctggctct ttttgacatg
      841 cggcagaagg accaggcatc acccaccagt gcagggccaa ttgaggtgca gattgaccag
      901 agggacagtg aagaagggca gccagcaggg cagcgctctt gtcaaatcta gtgatggaca
      961 cccacgtggg ggctggtagt tggcctacgg tttctgaggt cctacagcaa ttgatgggac
     1021 atgacgaggc aggcgccttg gtttcattct tttgtccctg gggatgtttg agggtgtatc
     1081 agaatgtcag agggtcttgg ctacagcaca cttctggtgt caactgcctg cgatatacaa
     1141 ctgccccttg cctgctgaca cattcaccaa caagaaaaaa gacactcaag tggctgtgac
     1201 gtactagtat actgtgtctt tgaaaaggaa aaacatattt ttttcttttc ttcattgaat
     1261 gaataaaaca tgaacatata gaacctatat gctgcgaaca cagattgctg aagctaagag
     1321 ggcaaacaat ctgaacacaa atgcactgac aaagtccttt gaagtagtgg aaacatgctg
     1381 ataagacaca cagataggcc tgatgtggtt aacaccacag catttacagg gaccctacat
     1441 gttcctacgt atgtgcataa gtgtgcttga gtgctcaggt gagggagaca attaatggtc
     1501 aaagtgtaga tctctataaa catatggaaa agcagctctg aaggacacat caaatagaag
     1561 caattcagct ccaacacaca aacacctaag ctgtcaatcc atatctcc
//
LOCUS       KCVB01000820            1570 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 38981pri ultra-conserved element locus
            uce-568, sequence.
ACCESSION   KCVB01000820
VERSION     KCVB01000820.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="38981pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1570
                     /note="ultra-conserved element locus uce-568"
BASE COUNT          489 a          300 c          306 g          475 t
ORIGIN      
        1 tttaagagga aaacaaataa cagaacagaa gcacagaggt agatttttga tatttcctca
       61 ccaatatcat aaaaaaacat gggacacttt aaatgatttg tgaaaaaatg tatgggtgac
      121 agcttcattt tgatttctgt attagctgtt tgttgctcgg tgtatgtgtt cctgagtgtg
      181 taccggtgtg tgtgtatgtg tgtgtgtgtg ttgtacgtcc agcagaacca aagagccatc
      241 ctcctgaact cctgtcactg cttctctgac tgggagttca agagctcttc attacagcct
      301 ctgcacatct gagggaaaga gaaacaatgc tcagagccca atgcacataa cctttccatt
      361 caatcagcag ttcaaactgt ctcccactac tgcaaatcat tctcttccca gctagtcaag
      421 tgcaccactg agtcgaaaag cctcccatca aaaatggata aaaatacatc cgacagtcag
      481 tcacctaaac gatcctttct ggaataaata ctgtgattga aactctgatt aaaaatggta
      541 atgacagcat ttcagacggc gcctatgcat tacgcatcac tgtctgtgat gtacgtatat
      601 tttacgaaaa taagtggtaa aactagcctg acagcacaat gccgatgtca aacaaaggct
      661 cgtcagtaat gttttataca aatccactga tataataatt atactatccc cagtctttag
      721 tggaggttga ttgttcttgc tgtgagctaa cacacttaat tgtagtatgc aaatgagagc
      781 aattacaaag cttttaggtg aagagatgac agctgaggtt ggttttattg gaggcctcac
      841 ccagttgtca tggaaaccac atagaataac gcaactgtca aacggatata atctcgtgat
      901 atgtccagaa attgcctcta ctctactttt tgttccacga actgatccat atgaaaagga
      961 gattctttga ttgccgagca aattgtcaat ttgtgttgtg ccgaggaaga gacggttcgt
     1021 ttgagaggca gaaatgttgg aatggattca atttccagaa ccaaagcttc gtaagaataa
     1081 aaaaaatgcg ctcgcacaca cagcccgaga cgctttgagc tttttgtaga gtcaacaatt
     1141 agacacatgt atcaaaaagg aatctccaac aattatttga aggaaaatat attttttata
     1201 gcactccatc aacacacaca tctgtttgtt ttgtgacatc tatagtttaa acaaacaggc
     1261 ctctttttta tgctgaaatt attgataccc gatgggttaa agggtattga tatgtgatca
     1321 ttttaatgct actgtaaatc aaagagacat atacactggc catggtatat ttagatgact
     1381 tcttattgta agaggaagtc accagagtta ccttttttta gtcggctgat cacatgacag
     1441 accttatgat ggtggtgatg gttgtcatcg tctgcaccgt gacgcaggac cttcccaatt
     1501 aaaatgatca cggccatttt ttcacaaagt ataattttct ttaaaaaaat atacattttt
     1561 tttttgtgaa
//
LOCUS       KCVB01000821            1478 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39024pri ultra-conserved element locus
            uce-200, sequence.
ACCESSION   KCVB01000821
VERSION     KCVB01000821.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39024pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1478
                     /note="ultra-conserved element locus uce-200"
BASE COUNT          430 a          304 c          273 g          471 t
ORIGIN      
        1 aggtaaatca ataaacacca ccggcttcat gaatgagcac gggtctcagc gaactgatac
       61 cttcacacca ggtatcaggt taggtctatt tgccaatcca ctgtgggatc tctggggccc
      121 gttctctata ttttagatta gatgtcacca cgcttaacaa tgagaagaca cctcactgct
      181 ctacagcaca aatccacatt gtgttggtca atcatgaggg taacatcaat tcatgtgaaa
      241 gcagggagag acggacaagt atgaaactac ccagatatcg atgtcgatgt tatctgacac
      301 aatagagcct ttgttattct gtccatccct tttgtttttc tctttaacaa gcttagtatc
      361 tccacatgaa ggctagttag gagacttagt gctcataaat gaatgtcttc cactagatat
      421 tcttcacatc ccgcaacttt attaacttca tattttccag cttgaaagta gatctctaac
      481 tttcagcaaa tagcattgga caagaagagg caagactgcc gaccagacag acgagcgttc
      541 cactctccag ctcttaacaa gcatagtaat acatcagtca aaagcaataa ttttactatt
      601 aatgaaattc caatttgcct tcagtgcatt tcacgttgac attttccaca ttgcaacagt
      661 gctttgataa agtttttaaa atgcaaaaac gcatctaaga ggctaaagac cagcccgggt
      721 gtcagcgtgc ctggcagagg actatggtac ttaattttta tcaccatgtg cccggggcat
      781 ttaaagcctg aatgttaaca ttggctccat tttcattatc atcatatgat tatggatttt
      841 actgcattta gctgataata caactaggag ctcaccttaa aagaagttgc tcggaggttt
      901 catttctgcc caaatgaaat tttcatttga caaacaagta taattaggct gcacttcttg
      961 ttcagtgtca gtctgttttc tgattgaact tagaccaatt tgctgccact aaatgagttt
     1021 gaataatttc cattctgaat gtcttgggga cattagcttt aggagtgcta agtggcagca
     1081 tgctttttga agttgctgat tatttgatgc cacccctgtc tacctatacg tttctgagct
     1141 tacttcaaag ttaacaagga aatagcctac cttggagtac aatgagaatt tgaaagtgtt
     1201 tttggccagg tctggaaggc aaagtgaaaa ttactttaat aagtgtgtta tacactttgt
     1261 tagcttcaat gctctttgct tcctcactct atttctttca tgtggccact tttattgtac
     1321 tgaatgagcg gatttacttc tgttgaaact gtacacctaa taactaatat tgcagtatca
     1381 gtttcattaa acccactaat tacaaattaa aacattatga gttaggagaa ctgctacagc
     1441 cggctctcac tctgcctcgg tgttattgat aataatgt
//
LOCUS       KCVB01000822            1554 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39102pri ultra-conserved element locus
            uce-694, sequence.
ACCESSION   KCVB01000822
VERSION     KCVB01000822.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39102pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1554
                     /note="ultra-conserved element locus uce-694"
BASE COUNT          383 a          364 c          387 g          420 t
ORIGIN      
        1 gtcacttata ttgcctgaga gaatatcatt ttatttaagc tccaaacaac ttacaagatc
       61 tttgttttaa tcactgcaaa gaaagctggc tgacagcatg tgctgtatat attaaattgg
      121 catcctgcgt tgatcaatgc tttttctatc atatttttaa gggtgagttc aaattacctc
      181 acagggacaa aatgttcctc accaccattc atatcctatc atgtttatca tttcgatttt
      241 aatagctgga aaaaccaaaa ctccggagaa gatgggttca gtgggataac tgtgagctga
      301 actggaacag tttgcaacaa actaacaaaa cgattgatag ctgtgatgac cgtctgttac
      361 tgcagggtga aaattaaggc agttgctgct ggtttctgtc ccagtccctg gagatgatgt
      421 gggccacaaa agaggaaggc agaaaaacac tggatgttgg gtagatgagt gatccaccct
      481 caggatctgt attgtttgtc ctcaccacgg ttctggtatt catgggaaga cactgtgtac
      541 cctgtgcggc tgtggaccag atgtggtctt tagttgactg cggagcagga cagggacgac
      601 tgtaggcact ggagacagag gggagagggg gagaaagagt ggtaaaatgt gtgcaagacg
      661 cctcccaaaa cacccttgat caacctcaac tcgcaggggg aaactgtatg tgtgtgtgcc
      721 tacgtgtgcc atggttggtg gtgagtgtgc gctggtgatg gctgttggag gtggagagat
      781 gttggggggg atgaggatgg ggtgtgcgtg gaaaccctga gaactttttt ttttcctctt
      841 ggcgtagctg cactgctctc caggaccacg gagtgttgcc cccctgcagc ttaagcctct
      901 gtctcttttt gtccccagca caacagcctg tggcattcca tggcagcaaa tctggctgtg
      961 ctgtcacatg ggggagatta gccaaactaa gacctcaata gaatgctcac tttactcacc
     1021 cattccagca gtggccgccc cccgcgccca aacaccctca cacacccaat ttaaccgttc
     1081 ctcgtctaga cagagtgcaa agcgattatt attggcatta ttaagactta tatgcaatgc
     1141 tctttttgtc attgtcaaat tcccagcggt acacatgagc tgccaagcaa tgagccctat
     1201 ttcccactgt gaaggccact tccgcgctgg ttgggtctgt gcagcgctcc acctggccaa
     1261 gctgtggctt ctgggactgt agacacaaaa tatcttgcag tggatttaca tctatttcct
     1321 atccatttta ttcagaaact gcggtttctc ctgccttctg gctcctccgg tccacctccc
     1381 cttcataatg cagttattct gggcttggtc acagatattt agagttattc tagccacact
     1441 gccactaccc acacacacac catccttatc tggctgggga cactcggagg aggatcttcc
     1501 aggaagcgat gcagcaggct ggaaaggtct gtggttgcct aaagggaccg agaa
//
LOCUS       KCVB01000823            1622 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39176pri ultra-conserved element locus
            uce-1133, sequence.
ACCESSION   KCVB01000823
VERSION     KCVB01000823.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39176pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1622
                     /note="ultra-conserved element locus uce-1133"
BASE COUNT          433 a          312 c          384 g          493 t
ORIGIN      
        1 ttaaattgtc ttaatgtccc cacactttgc aaacgctata attactaaca cttcaactaa
       61 ctgctaatgt gtctaaagta ctaatatata tctttagttt gctctacttt actctctgtc
      121 acatccactg tgatttcacc tcaacagtgc aagaaaggtg aaatctgaat gtgtgagcac
      181 caagatttat cgtctgcatg caaaaaaaat atcaggatca ttaagtccaa ctttactgcc
      241 atcaagctga tgaaagctgg caaacacaca gacttcataa tgcctggtgt gtgtgccagt
      301 gtgtgtgtgt gtctgtgtgt gtgtaaggga tgtgataagg tagattgact tctaatataa
      361 acggtagagg tgtctactga catccatata gcagtgccct ttgtgtccct gccaggcaac
      421 ccctctctct ttgtgtgtgt gcgtgtgtgt cagaaagggt atgagagaag gggcgattgt
      481 tgtactgtgt gtatgtttgg ttgcaaacat aagaaagatt acagtgctgt tcttcttctt
      541 gtctctgtga tcttttatgt ttgcaaataa tgtattaaag cttgtgagtg tatgcatgtt
      601 tgcgaaggtc tgtgagtgta agacaatgag tttgagtaag tgagggggca agtcggtgga
      661 cttgcacaat atgtatacgc aaatttctct ctctctctct cgctctctct ctctctctat
      721 cttacagaca ctcctcacat agaacataca cacaaacaca cactggagca ggttagccct
      781 cctccacacc ggcaactgct ccgctgaagg tgaaagaagt gctcacttga gctcattgaa
      841 aagtagatgt gacagtaaat ttttcaggcc cttgtgagat gggagatctt gttatagcag
      901 tcaggatggg ggtcagggca gtggctgctt gatgattgag gaaaagagaa gtaaattcat
      961 tctgcttcgg gcactttgtc aagtgccagt agcttactgt tgagagccac tcacccatca
     1021 ccgtgttacg gccccagagg tcacctccac cgccataaat ctgccttgcc tgctaaacct
     1081 actgccttcc ccgattgaac acaagcaggg agagaggctg cagctgctgc tgctgctgct
     1141 gctgcccctc atagtcactc aaatgaaaaa aggcagaggg gagaatatag attctccttt
     1201 tgatgctgct gatggtgtaa gagcaggaca ctgggtgtgc tctgccaaag ggggatgtgt
     1261 acgtttggac taatggttgt gtggtgtgag agtgtgtctg aaaggataaa tgcattacag
     1321 atatatattt gtagagaggg gcatttttgt ttgtgttaca ataggggctt gcatgtgttt
     1381 gcatacactt tttgtaattg ggagtcatat agtgtatttg tgtattcaga ggagggtttt
     1441 tggagctcgt ctgtctaagt tcaaatcagt gtgccccaag ttttttttag acttcctaaa
     1501 aattatgatt aaaaaacaga tttgtgcata gacatcagag ggacagaaaa acataacaaa
     1561 acaaggctga ttattgcaaa tttaacacta gttgtaggtg cttttcacat gtttttgcaa
     1621 ac
//
LOCUS       KCVB01000824            1198 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39210pri ultra-conserved element locus
            uce-669, sequence.
ACCESSION   KCVB01000824
VERSION     KCVB01000824.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39210pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1198
                     /note="ultra-conserved element locus uce-669"
BASE COUNT          390 a          189 c          269 g          350 t
ORIGIN      
        1 agagagagag agagagagag agagagagag agagagagag aaagtcgtag gagaagacgt
       61 aggtagacaa agagagggca aggaagagga gaagtaagaa aaacaaaaaa agaaagcttc
      121 aactttcttt aattatgttt acccaacagg ctttgcttcc tcatttaaat agcataaaca
      181 tctggccgtg gcgcgccact agcatgacgg cagtagtcaa gcaaatcttg tggaaaataa
      241 tgaaattaga tccctggtgc tgtgacagtg gaagggcaga agacatccgc tccatgttaa
      301 taaaattgcc atgaatgagt gaatacaatt aggaaattgt cagtggcggc agtgaacttt
      361 tgtaactcac cgatgtttgc atcattcaat taaggcagca atttggactt attatttgtg
      421 gctgttgtgg cttttattaa ttgtgacccg gcagtgattg gcctgcgatt aaaccagtca
      481 catatgatgt ggctgagggt gagggatgga ttggtgggca agctgatgca ttttttattg
      541 ttgtttatct ctgtgagtgc agagtgagga tgacagaaga tagcactagt gtgtgcgtgt
      601 gcgtgtatgg tgtgtgtgtt tcccagtttc agctaacttt cttctgttgt aacctgttta
      661 tggatatcaa tcagattgca gaagaagctt tctgtatact gaccttagta tactgtaaga
      721 tgtgctgccc ttgagtgctt aaagttaatt ccaacaaaga cagttcattc tgactagtgc
      781 taagaggact aaggaaaaaa ataaacaaaa actattttaa ttcatgaata attgcttctc
      841 tcaaatattc agcataaaca tactctggtt tcagcttctc atatgtgagg atttcatgta
      901 tttaaaagtc ttttgatttt agtctgctga tcagataaaa caattaatct gaagatgtca
      961 ccttgggctc tgggaaatct tgatgggcat tttgaacacg tttctgacat ttaataaaca
     1021 aagcaattta tcaattcatt gaaaaaaata attggcggat taattgatca aacgaataag
     1081 cgcaacaact ttcgatccaa cgttccattc attacagcag tatcttaaaa caaggcggaa
     1141 taaggtcaca caactactac catgacattt acacttgatt aaaaacaaaa tgttttga
//
LOCUS       KCVB01000825            1871 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39247pri ultra-conserved element locus
            uce-101, sequence.
ACCESSION   KCVB01000825
VERSION     KCVB01000825.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39247pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1871
                     /note="ultra-conserved element locus uce-101"
BASE COUNT          491 a          383 c          483 g          514 t
ORIGIN      
        1 ttgggctaaa acaggtgggg atataaattt aaaaatttat taaaatcttg aaaatttgta
       61 tgtgtgtgca tgcttgtgtg tgtgtgtgtg tgcgcgtgta tgtatgtgta tgtgtgagtc
      121 cagccatctg acgctgcaca agtgcgtgaa tgtatgcctt aatgtgcatg tttgcgtatg
      181 tgcctgtgag tgtttgccgt gttcgccagc ctgctgctgc acgtcctcct aacggggaga
      241 gtttgaaaac ctccctcctg ctctgacatt tcagccccca gcatgccagc cttgctatgt
      301 gtgatatttc tcctggcgtt ggggtaatgg caaacagtgg cccagactcc caggggatgc
      361 ggccgtggtg acggcagagc aacaacacgg tcacttccca caatgctaca gtagtagaca
      421 ctcctcagac tcccctcaga ttccttctat cactcccttt cctccactca tctctttatc
      481 cttcgactct ccaatattgc gcagcgcatt agtcctacat tttcacattc cccaagtcgt
      541 gcctctgcgt cattatctaa gagagattag gcaagatcaa aggtagcttc cgttatttta
      601 ccaagaccat ctgcttagga tatcattagc aactgtacct caccctgaga gtttatatat
      661 tcagtatttt cacacgtttt aacaattagg cgactagtga agtgctggga tgatatatta
      721 ttatattgct ctgagtatat ttgtagagtc atggttgaaa tgcttttaag tttgccagcg
      781 cagatgatgg caaggcattg ctcattaatt tctgcacaac atgtattaat aagtatgaac
      841 gtgtagcaag atgaacttta atttcccact tcattaagcc caatgttatt aatcttgggg
      901 cacacactac ggctaagctt gttttcaaag caatcatgtt ggtcatgaca catgcacaaa
      961 acctaataaa cataattact aaaacaattc tgggaaggtc ttttcctgcg acatcagagg
     1021 ggatcatttg tatgaaaggg caacgaggtt gtatgtttgc atgtgtgttt gcacagacgt
     1081 gagacggcag tcctgcacag tacttgcgtg tcgtgtgtgt gaggatatgc tctgtgtgag
     1141 tgtttgagtg tgagctgaaa ggtgtaaatt ggcctgtcaa aatgcatata tttgtaatga
     1201 ttaatccctc accaaaccgg acagaggcct gagcagcggt atctgaccag tagtccgtag
     1261 ggaagggatg agggggtgag gggggctcac cttgcagtcc gttgccgttg gcgctggtgc
     1321 aggagggggg tggggaggcc tggggtcgga ccacaggggc gaaggcgctc ttttgtttaa
     1381 ctgcagagac gaaagggagg gaaaaaggaa ggagggaggg agagagtgag aacgtgctgt
     1441 gagtagtgct ggagaaggag agggcagtta aggtaaggct aggatagtat tactgccagg
     1501 ggggatacac gatcagtact cacaggcccc taccatcaac attttgtccg gttgaacaaa
     1561 tacaggtggc atatggtgaa gtacacataa aaatgaatat ttctcctact ctggatgtgt
     1621 gttctccgaa gtactaaaac taaacaagcc cccctgaatg aacagtaata tattatcacc
     1681 atcaataatg ttataaccct gtcagaagtg attcatagaa gtgaaaacga atgtgggagc
     1741 tggaattaaa tcagcagcag cttagtgcta ctacagcgtg cgatagtggc aagctactca
     1801 cttccatatg gggagttggc agaggatccg ttgaggaacc caggggagct tggcactccc
     1861 aggttcatac c
//
LOCUS       KCVB01000826            1686 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39292pri ultra-conserved element locus
            uce-595, sequence.
ACCESSION   KCVB01000826
VERSION     KCVB01000826.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39292pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1686
                     /note="ultra-conserved element locus uce-595"
BASE COUNT          444 a          360 c          415 g          467 t
ORIGIN      
        1 aaaatggtaa tttttacact tttaaaccat aaaaatttac agtattaata agtgaggtac
       61 agtgggcaaa ttttgtaacc tttgaacaga gccaggccag ctatttcccc ctgcttcctg
      121 tctttatgct gagctaagct aacaggctgc ttgggatatc ttcatattta atggacagat
      181 ttgagaatgg tattgatcct ctcatctaaa tgatcaccag atagtgaata agcgtatttc
      241 ccagaatgca aaaactaaag aaaacagtat aaattgtttg acatatttga ggcaggaaga
      301 tttttgtcta tgcataaaaa ttgtttttct tttttggtac agcttgacta caagtattta
      361 attcattgta tcagccacgt ctattgatga gtccagtcta cctccaggga gcgcactgct
      421 agcaggtgtt tcccctgtgt ccggagctaa aagatcatct ctatctatag tggtgggctg
      481 tgctcctgtc tgtaacgtga agccccccag tcacactaca caaaagcagg actaaggggg
      541 aggacagcat ttacaatcag gctgaatgtt tggcaccggg tgataaacat gattgtgtaa
      601 tgcctctgta tgtgataatg cgaatattgt gctgtgagga gcggtgggga tgtaggtggg
      661 tggggggtga caagaaaatg agggattgga ggaggggagt gttgaagggg gagttgcatt
      721 ctcaaaatta aaagttgtag cctattagtc tgtgtttggt agctgagcac gcgcccatgc
      781 ctaacacggc tgctaatagg aaaagatgtg tggaagtgta tgatttatgg ttgcacgcag
      841 gcaggggtgg aataatttgg tgcagaacgg gtacttactg aacggcaaca actcggctac
      901 ttatccgtac tttctggtga taaattagct cttgtttaaa gtagccggag acccattttt
      961 cattttgatt actacacttg atatctcttt ctttcttact ctctctttgt cagcagtggt
     1021 tctttgcatt ttctgtgtat ggtttggtgt acggtgtagt gtttgtgtgt attcagccaa
     1081 gccgctggtc cttaacccct cagtgactgg acactgcagc accctcccat gtaaaaacaa
     1141 ggtaatgttg ttaattgtga ggaaacccaa tatggccaga atgacatggc ttttctcatt
     1201 gaccgtgagc tgtcctgctg ccagctggat gcaaggcttc tgtcaatcag cagccgaccc
     1261 ctgccgggac cttcccctgc tcagacaagg ttcccattga tccctccgtc gcccctctga
     1321 caggaagcag cctcagtggg ggaaataagc aggtgaaata ttaagttata agtcagcttc
     1381 caatcaataa acagggccag aagctgggag gggcagggcc aagtccccaa agcgggagtc
     1441 ctactagctg ggggtaatac gattgaggtc tgtggatcac cccaaagacc tgcaggcaga
     1501 gccgaaatca tctctcctct gcctgactga agccatcaca ctgagtccac tgccaagtcc
     1561 tgcagcgcta gctggcaccc gctcttattg tcctctgttt ggcactgcca cccatgccag
     1621 tgcccaggca gagctggaca ctctacagac agcataaata aaacatatga gagctgtaat
     1681 gagaaa
//
LOCUS       KCVB01000827             928 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39337pri ultra-conserved element locus
            uce-907, sequence.
ACCESSION   KCVB01000827
VERSION     KCVB01000827.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 928)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39337pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>928
                     /note="ultra-conserved element locus uce-907"
BASE COUNT          270 a          183 c          193 g          282 t
ORIGIN      
        1 acacacacac acacacacac acacctcaga tcagaagtag tgtggagtgc tgaggctgca
       61 tatgtaaatg ctgagagatt gtcaagtgtc tgggtgtgca tacatcaggg taatcaagcc
      121 agcgctgatg gggagagctc gctccaggag cagatccgca gtactggcag tcacacagcc
      181 gtcactgaac tgctgccaat aatgggtttg agaggtgctg gaggaggggg tagagagcat
      241 gtgtgtgtgg atgtgtgtgt atttgtgttt actcgcacac aaacatgccc atctgcaagt
      301 tgccatctat tttgtatggc atctgtgcat gcatgtatgt taatgtgaca cctctatgat
      361 agaaaagctg attgagagtg tgaaggtgaa gccaatctgc ccacagaggc gttggagttg
      421 catttggagt gctgctgagt tacaatctga tagaaaaatg cccgtggata aactggccta
      481 gcttcacctt cctttgcaaa gctactgtgt ccaatgcaat aataagcttt tacctccttt
      541 ccaacctcat cctacatttg taccatattc tactgttttt gaatatttga agaatgaaac
      601 aatgaaacgg aatctgacta ttgatcagtt agtctatcct gccatactga tgaatgtcat
      661 atttctttct acaaaacaat actatactgt accctattat acctgtcatg tcagggttta
      721 ctgtatagcc taaaatcagt ttaatattgt ctgatggtac aattactcaa gcactctact
      781 acaggacaac tttatttttc ttttctacaa tatttccagg ggaatattga cattttgacc
      841 agttacaaca ctaaaatgct aatattactt atgtgctaat gcatgtaatc agtacttatt
      901 agacattatt aattataaaa tgaatcat
//
LOCUS       KCVB01000828            1758 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39382pri ultra-conserved element locus
            uce-285, sequence.
ACCESSION   KCVB01000828
VERSION     KCVB01000828.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39382pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1758
                     /note="ultra-conserved element locus uce-285"
BASE COUNT          479 a          369 c          365 g          545 t
ORIGIN      
        1 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg taggaatttg
       61 tgtgtgtgtg tgcctgagag cagataagat gtaaccatgg gattcaaaaa tgtaaaggca
      121 atagaatggt gtatttgatg aggttaatta attgtgattt ggatttcatg tctcataaat
      181 aagaatgtgt ggtaggttgt tttatttttt gtgtaatttc aagtgaagtt tggtttgtgt
      241 ctcattagca cttgctgcag cattaatttg aaatgctatc tagctctggc accgctcttt
      301 gttcagagct ttaaaatgtg ctggtattga tgtcattcct tctccgtcct tcagtctatc
      361 agacaaagac atggaactgc aatgaaaaag aacaggttaa gaagtttctc tttcagcttt
      421 gttttgttca cacacaaaag ccgccgcttc ccacaatcat ttttttcatt ccagctatct
      481 tttaatgtta gtattttctc actccttgtg cacatcaagg taaagcgaga cttcggtttg
      541 ctatctgaat atcaaatgct cgttggttta caatgcgcca caaacacaaa agtcatgttt
      601 atgaattttg ttgcaaacag tttgtgtttt ttgtaatcga aactaatcct tgacaggtaa
      661 aaaacaaaaa aaagaggcaa caatttatcc tacatacttt atatcactta acagtgtgtg
      721 tgaaggtgaa agtaaagata agtgttctct caacctagtt ttggttcttg acaacaacat
      781 tacagcagtg ttactgtttg ttgcagcagc ggcagagcgt gtttatggac cttgaagtcc
      841 actcagagga aaaaaaacaa gactgttttg aatgaaaaga aaaaaacctt ggagcacagc
      901 cttgtaaatt aatttgttcg tgttgtatga tgggaacaca gctggcctca ttaacatgcg
      961 cacaacaaat aattatagcg ttttttttct ctcttcagcc agccaatgct tggctaatgg
     1021 gcttgctggg ttaattatgt cagagcgtaa ttacacaggg cccagggaga cataatggtg
     1081 caccctgctg cgcccacacc gcctaatgat ggtgtatcgc tgacagggcc aagacggacc
     1141 gccccgacgc acctgcttgt cttggcgcac accaccctgg tccaccccca cccacctaga
     1201 cacacatcct gaacctgtgc accgtcctcc ccggtgccac ctgcaatcca ctgagccgtc
     1261 cagcttgtga cggactttgg gtcctggacc gaagactcat ccagatacag taactgcctc
     1321 ctactttgag atgtgcaata gcacagaaac agcaacgcag agaccactag gaagcggcta
     1381 cagggtggca gcctcattat ggctcagcca gtgtgatgaa actcagtatc aatgcagctg
     1441 aaaatgtgca aaaacaatta aaagtcatgc ttactgtgtt ggctattagc aagttagggt
     1501 tcatcacaac tctggtctta tttttgtgct tttgcccatc tcttctgtaa cattgtattt
     1561 ctaatttttc actttgagaa cacacacaca tacattatgg cccctgcaag cttcaatata
     1621 agaccatcag aatgtgaact cctttattca ccatcatttt cactgcctta ccttcatcca
     1681 gtctccttat atgctgccca tgacctgaat cagtcatgta tagattacca tggatattaa
     1741 ctatgtaaat atcatttt
//
LOCUS       KCVB01000829            1584 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39422pri ultra-conserved element locus
            uce-654, sequence.
ACCESSION   KCVB01000829
VERSION     KCVB01000829.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39422pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1584
                     /note="ultra-conserved element locus uce-654"
BASE COUNT          387 a          372 c          351 g          474 t
ORIGIN      
        1 gatccaatca tttcattatg tttctataaa tggctgcatg ctgctcgctt cgggctgctc
       61 gtctcgagtc aaccatcatg tgcgcgtgca tcctcccgtg aatgcgactg atggatgcga
      121 gtgctgcggt ccgacagtaa cgccttgtcg ctatcattac cgctgatccc cccacagaca
      181 gcgggagagg aggagaagag gagggcggct gttgctcatc acaatctggg taaattgata
      241 cgtgcagcat gaaccgacaa accggtagga catttgccat ttggcttctt tgctgtttgg
      301 atggcgattt ctgcgcctaa acgcacatat ttatgcgccc ttttcctcga ttgaaacatg
      361 ttacattatt gtggttgcat tgcacggaag ttgaccccgc gcgtcatgat cgtgaagtgg
      421 tcgtgcattc cgcaagacct cggttattgt tgtttaaatt tggctttaat cactacagga
      481 ttttttgtgt ttattgcttt attgatcagg ttacattctc ctctttcagg ccttcttgga
      541 tctgcacctg tcagatcttt tcactctctt gtgatttctt gagtcttgag tcttgagatg
      601 attgtgtgtg tgtgtgatga tgatgatgtt ccagcttaat gcagcaataa tcctccatta
      661 aatccccccc ccacctccct tagcaagctc tcactgtggt ctccccccgt cttctcctcc
      721 attctccagt ccatgggctc agagcgggtg gagatgcgta agagacagat gcaggttcat
      781 caggaagcag ctgccagtgt cctccaagcg cgccacagga tgggaaacac ccccacaaac
      841 gcctcaaacg cctgctgctt ctgctggtgc tgctgctgta gctgctcctg gtaaaaacac
      901 cccactgctt cccacacagc agggccagag acaggagata tgtagtctaa gagccatttt
      961 acagtcttct caataattgt tttttaaact gggctcattt taggttgtaa tgaatggttt
     1021 tggctgctaa tttgcagtcg tccgtccatc cattcatcta ttatatcaac atagcatgat
     1081 ggctgtttgt gaaattgtca cattggattt gtggacatga ggcacaatga tctttaaagc
     1141 ctgaaccatg tttctttgag tgaatgaaac actacattta caacaatatt agagtgttgc
     1201 tttcaagttt tttagtccaa aaaataggca ggaaaaatac acagagaaaa atcgacagta
     1261 tggaggcatt tcggtttagt aaaacagaga attctaggcg gaaaattggt tttcttgcac
     1321 gtagatttgc aattttattt atttttaatt ttttaaaagg ccagtggaaa atcacacata
     1381 ctgcacttgt gatgcgctaa atgtaatctg acacagcagc agactgcgtg acgttatact
     1441 gtgaaaatgt ccctctgggc ctcatttgaa gggagcagat caatagtgga tactacttcc
     1501 ttttttgcct ccatccctcc acccgtttga cacaccacag ccctcgccat catcccagaa
     1561 aaacagagac acagtggtcc tgtg
//
LOCUS       KCVB01000830            1320 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39462pri ultra-conserved element locus
            uce-510, sequence.
ACCESSION   KCVB01000830
VERSION     KCVB01000830.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39462pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1320
                     /note="ultra-conserved element locus uce-510"
BASE COUNT          353 a          280 c          261 g          426 t
ORIGIN      
        1 attcaacacc attttttact aattttgtct gaatttttag tcaaacacat ggcacaatag
       61 ctggaatttg taactacatg tatatttttt gctaataaat gttatttgca acaaaatttt
      121 ttcagattct cttctggaaa aaagaataaa atcacagcct gcttgaccat ttgctattaa
      181 tattcacatt tgaatacagt ctagactaaa acagaatgca aagtggagcc atcaattaca
      241 ttaagaatta atggttattt cataatctca tctgatcatt tctgtccaga tatggctgct
      301 gtcaccgaaa aagcagggca gagagacgaa ggccactttt tcatcctcct ctccaactgt
      361 ccaaaaccca ttctgtgtga cttcataggt cacccctctc tggtgcagtc actggtttcc
      421 ctccctcccg tctctcacct tcccctcctc cccctcttta atactttctc tatggagtaa
      481 gtcgggggga caggcggtca cgcagtagct ggcttcatga ttaatggtgt ttattcttga
      541 cagaaggctc ctttatttat ctgcattgca gtttcactga tactatcagt cacctgaatg
      601 aaaaatattc agtcatacca attttgcagt ataattgagt agagtcactt cctttcagtt
      661 tttttccccc ctcgcctctc ctctcctact ctatctggca gccttatcat cgccacagta
      721 attttcgcgc tgcgttgttt gatatggaga ggctggcttt agccgtattt gacataaagc
      781 ctcttcaatt ttcactggag aaaaaaaagg gggaagaaag aaagttgtgc accactctgt
      841 gaaaatgtgc gaagaaggga aagtatcccc tcccaccccc cctttttttc tcaggcaatt
      901 tcaggattta gagagaaagg agataaatgc ctaagcagaa atgtgggcag attgaagtta
      961 cagtatatct attttttttg taatacacca gttttagaac cctaaatggg cagtgtagtc
     1021 tttttttctt ttcagcctgt gcacaggacc gtgatatttt gaaggcacct gtgtattttg
     1081 agaatgatgg atccagcagg ttggagggaa gaaggggggg aaggagtttg gcacagcaga
     1141 tatttgacaa ggcctgtaaa ggtttaaggg ttgagtcatt acccgtggag atctgttaag
     1201 acgtgttcca aaactctact taccgggcta ggattaccta cgctgaggct gctcctgctg
     1261 ctgtgtgtat atttgtcttt gtccctagtt attaaatatc acacgcattt ttaatgtttt
//
LOCUS       KCVB01000831            1395 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39506pri ultra-conserved element locus
            uce-982, sequence.
ACCESSION   KCVB01000831
VERSION     KCVB01000831.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39506pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1395
                     /note="ultra-conserved element locus uce-982"
BASE COUNT          421 a          274 c          289 g          411 t
ORIGIN      
        1 tgttacagaa ctgttcagaa aatgtgtaaa gctccgttgc tcttgcagaa gaattttttt
       61 tccccctggt agtaactttc aaagggttgc acaacattaa aaagatggaa tgctacaaca
      121 aaaagcacca tggtgttttg gagtggtttc aaatccataa tattgaggtc aacactttct
      181 gaataaccct agtggttgct gtgtgttctc catttgtaaa aactctccaa gtgtcatata
      241 atctatgctt gtttgaatta tatattctat atattcacct gaatttcaac caacaaaatt
      301 atacatttag agtactgaaa aaggatgcta tgtcacttca actgagcaat tctctactgt
      361 gtttatgagt tcagaaggac catcaaattt catgtaaaat aagctctcaa gcaggagaag
      421 accatcatat tttatgtcta attctggagg agaagatgta tttcctgctt ccccaggaag
      481 catgattggt gtgtgactgc gtgtcgtgac gagatcactt ggcggggctg aggataaaat
      541 gtcgcttagg ataatgtgat tgcccttaag gtacaaggag gtgaattata ttccctgatt
      601 tataatctga aaattaaaga ctcactcatt ttctaattta attccacctc caatggaaca
      661 atgttcactc tgagccaaat tggcgaataa tgttactaat tcagatgcag atgtaatttc
      721 tattcggggg gctgcggcgc atggtgatga acgagtcccg gatggatcgt tgccactcct
      781 atctccccgt ctcgacaatt ccccagcagt tgtgataata acctgagcaa taaagggaga
      841 gaggcagtgc taccctgttc cactcctcat accccaagtc atctgatcat ctaatagtat
      901 ttctgtcctt tcctgtgtca ctctgtcttg aggcagctga cactgcgctc ttccatacag
      961 gaaaacagag tatgccgaat attatcgatg taaaactgca ttaacatgaa gactcttaat
     1021 aacctgcata attactgctg ccttctgcaa tacaaaaaag tcattgcaat aaatctgctg
     1081 ttaaaaactt gattgaatgc agggtaaatg tcaaggtaat tatgaggcgg tgagggttaa
     1141 aagcaacgtt aaatcctccc acagagtggt gacattcaag agaaggagga cgagatagcg
     1201 aaggaggaag gaggagagcg acagagagag ggcccagttt gtaaatgtat cagccaatac
     1261 gaatctaacc gttcctcact tgtcactgcc cttttattta tgactgcgaa ccatttgaga
     1321 tatgacctct gtatgattgg tttcttcctg cacgctaatg gatccattaa ggacagaatt
     1381 cgtcatgtaa aatat
//
LOCUS       KCVB01000832            1587 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39551pri ultra-conserved element locus
            uce-555, sequence.
ACCESSION   KCVB01000832
VERSION     KCVB01000832.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39551pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1587
                     /note="ultra-conserved element locus uce-555"
BASE COUNT          476 a          319 c          315 g          477 t
ORIGIN      
        1 agggcatgta gatacagaac tacacccact gcaggattac ccagtgtgca tccccatagt
       61 gtaaatgtct tggtgtctgc tctgcttata gggctagtgg cataacataa gtaaatatag
      121 cttctgtgcc ttgactacag tgcacgtgtc tacatgtctt tcagttctcc tattacacac
      181 aaaataattt ttgcattttg caggaaatag cagtcagaag taaagtgctt tcattaggtt
      241 actgtcagca ggacatgcaa aaatctaaag agcaaaaaaa tgttgagact ttatctctga
      301 aaaaaggaaa aagaagttga aagtagtatg ttgtgacact cacactcacc atcagttgtt
      361 tcatggaggt gtgttcttcg ttttcccgtg ctgcattgtg gtctttgtgt acaatctcca
      421 cccggatgtc attttttgca gacacaactc ctttaagatc tggcagaaac agatgacaca
      481 tatttcttaa taagccggat aaacaccact ctgtatttgt tgactttata atttccccct
      541 agtgcagttt gctttattac agaatttagc aaagatccct ggttttggtc tgtgagagct
      601 tccccatttc ccgaaaacaa tgcaaagcat tattaatcat ttgtttcctg ccaacacaag
      661 attatctttt tctgcataca tatgcaatct caaaaagcct tttcacggtg gctcattctg
      721 tttgggacaa tgctgatgat actgtcatat aacaaagtct tactttctgt cttaattttg
      781 cttgcaagtt ctctctggtg agcacagatg gagacgggca gtgagggcat aaccaggtgc
      841 gcttcctctg tagtatacag acaatagaca tgctgaaggg accagtagca caaattgggt
      901 tttggataga gacagctgct tactgtctct aacctgagtc acttggtaca tgggtgggtg
      961 gcagagaaca taacgtatga tgtaaatagc ctcaaaagtg aatgcagcca gactgagatt
     1021 gcatgaaatg agtccttatt acaatctggc tatctctgca ccacaaaacg atgtccatct
     1081 caatgagtga tttcatcttg tattaagtgt gtcactatgt tccagcaatt catcttgtct
     1141 cagaaatttc ttgaagcagc tggtaatttc tttttttttt ttctcataga tgcacacata
     1201 tttaaaaaac ctcaagttaa aacttgcaga caaccataat tgtttaacct tcagagcaga
     1261 cattttccat tattttttat aataagaaaa aaaacttcta gatttgaaga gatggacatt
     1321 tttcttgtgt gcttgggggc gatttcagta ctttgacatg catgctttag tggatgtaac
     1381 agggaaagaa gacgtacttc tctgggaacg ggtgcaacag aaagctccaa tggtgcccat
     1441 gagcacaatg aaggccacaa aagctccaac agcaatgccg atgatcaccg ccacagggag
     1501 agactctgca gagagcaaca aagacaagtt tcctacaatg cacaatatgt acaattactt
     1561 accacaaaat caactttaca gggtaag
//
LOCUS       KCVB01000833            1516 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39589pri ultra-conserved element locus
            uce-278, sequence.
ACCESSION   KCVB01000833
VERSION     KCVB01000833.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39589pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1516
                     /note="ultra-conserved element locus uce-278"
BASE COUNT          517 a          273 c          354 g          372 t
ORIGIN      
        1 aacatttttt tttctttgtg ttttctttta attgcaggcg ttgtgtcagc tggagaatac
       61 atgaaacagc agttaaaatt tgcttcagct caatccagtg tgatttgcct catcagccag
      121 caaattcaca gatgaaatag gacgacgttg caacttatac ttgaatgttt acccagtccc
      181 ttggtgagtt gattgggatt agttcatatt aatgtctgtc aagacataaa aatggaaggg
      241 ttggagaatg ggatgctgaa tggggggatg aacagaggga gacaaactca caacctgaaa
      301 tgtcacgccg tgggtatcac ccttagtcct gcgctcctag cactctgagg aaacaaacac
      361 acattcacta atgagacgac agtacaccca agcatgggag gagatatcaa gatgaatttg
      421 aaatccagta tgaacagcaa aaaagacaga agaaagagag gaaatgaaac aagcagatag
      481 caagaaagaa aaaaaagaag actcttcaga gatgccaacc ccgaaatgat aactgttttc
      541 agattaataa gccagaaaga taaggttttg ttcagaacat gatggcaccc ctgaagcaaa
      601 gagagcagag aagcagactg acagagatct gactcacggc cagtagtggg aggagcagta
      661 gcagcagtag tgcttgtagg agaggaaagg gcattggaca aggacacgtg actaggacta
      721 gaaacattgg ttacatcctg ggtctggctg gtggtggatg attggcgtct cagagccccc
      781 gccccctgaa aggaggtgcc actcttgaag gtgttgacta cttcaatctg tgggaggaga
      841 gcgggacaga agaggacaaa tggctgctca gagtgacaga gaccaaatgg taacacaggt
      901 tgactggctg ggtgattttg gtggtaaagg gagcacaaag tgtgtcaatc atatcacgat
      961 gggggggggc gcaaaagctc agggtgcagt atcccctcaa tgaagtgctg aatttgactc
     1021 aaagggttag ttcacccaaa ttacaaaaaa catcttttct cttaccttca gatttctgcc
     1081 aacacctaaa tacaacggag gtgaatggat ttttcatcgt ggtgctcaca gcgataacaa
     1141 attaagttaa aaaaaaacaa ctaataattt aagtttcttt tacagaaaca atgttccact
     1201 ttactaagga aacagttcta taaaaactac agacagcaca ttgagtggat tatgcagagt
     1261 aactgggaca ttttatctgg aaaaagatgt cataattttg ttgtaatttc actgggtgtt
     1321 gaaaacagaa atcagaaaac ctacaaaact aaaacaaaaa ctatcagcac agctaaatac
     1381 cataagaggt aagacagaaa atgagttttc taatttgggt aatcggcccc tttaaactgg
     1441 cttacagatg cagccattta cattattcaa ggtatccaat cattttactt caggaatttg
     1501 agaaaactac atttta
//
LOCUS       KCVB01000834            1560 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39627pri ultra-conserved element locus
            uce-448, sequence.
ACCESSION   KCVB01000834
VERSION     KCVB01000834.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39627pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1560
                     /note="ultra-conserved element locus uce-448"
BASE COUNT          522 a          311 c          287 g          440 t
ORIGIN      
        1 ctgttagcag cagttaactc aaggaacaaa ggagcaacca gcggtcttca gagacacaga
       61 agttaatgtt gaatattttg acatttgtca aaatatttaa aatgcaaaaa caaaaaatat
      121 aaaaataaag tttccgaaca atcataggag cctcgtgtac aaagaagtca caattacatt
      181 ccatttgagt ggcaccaatg tgaaatacta attaatgaaa ttcagatata aacattctct
      241 ccctcaaaat gaattttata atgtgactgc tcatataata ttgttttgac ttccaatgct
      301 cggcgtctgc atctcagagt cagagacagt gacaggtaaa aacacacgct atatatgata
      361 acagacattt caatgaatag ctgaacagtg ccattagagg ggagaggggt gtggagctat
      421 gcagatcaca gagcaataga aagatgatat tgctattacc aggtgttgac acatgagatt
      481 gcatttacat tttcatccaa tggaaaccta aacacggact gttgtttgtg agagttagcc
      541 acaagtcgtg aataccaaaa cagtaatcaa tatggagaat aacttttgga aattgcattg
      601 taaaacatgg aaaaactcta acatgttacc ccatctttac tcctcatctc tcttttctct
      661 cctcccaagg cctttggctg ccgactctca tcctccttca cttttcatcc aacccactct
      721 cactctctgg tgcttgaata ggttgacaga cttctctgtg tttgtgtttc gtgtagtcaa
      781 tggcaatggc gttatcaatt agtgcagcag tgggagccca attaacagca tcaaacagct
      841 aattaataga gctgtgtgag ttagtagcgc acaacagctc gctgcatgac tctaagctag
      901 cgcagccatg ttatagcttg agtgtgctgc aagggagaca gggctacagt aacatatagt
      961 cacatgcatg cacacacatg cacatgcaca taaacataca tgtatagata catgcacaca
     1021 ctgatacata ttgcaaacat gttctttata catattaaca aaggtaaata tgccaactta
     1081 cctacaaaca tacagctgac acaaaaatgc aatcgaacac aaaattgcac acaagtactc
     1141 aaagatatag aaatccaaca atgacagagt aatgcactta agacatcaat gagttaaaca
     1201 gcttaacact ttccctcatc tttttattat taccagcatg ccatggcaca gctaatttgt
     1261 ctgaaattat tttgctgcat tcagacaata agttagtggt gaaagagcag ctcgttcttg
     1321 agttattttt tttgttattg cagtaaaact gctgagaggt gcagtaaaaa ctttgggttt
     1381 gggctgtcgt tcagacaaaa caatgtgtaa acgtcacctt gggcattagc aaactgacag
     1441 ccaattttca atatttctga catttgttaa atgaaacaat cgataatccc gtaaaactct
     1501 agtgggaagt ctgcattttt gtctgatgac ataagtgagc aagagtcggc actcttatcc
//
LOCUS       KCVB01000835            1556 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39664pri ultra-conserved element locus
            uce-1312, sequence.
ACCESSION   KCVB01000835
VERSION     KCVB01000835.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39664pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1556
                     /note="ultra-conserved element locus uce-1312"
BASE COUNT          420 a          316 c          334 g          486 t
ORIGIN      
        1 aaccacttta attaatttct tcactttgtc tttttccccg cctccctgcc atcgcacaca
       61 tgcaggctac acgcaggagg cagtaatata atagaaagta cgtgtcaaca actttggact
      121 cgataaagag aggattttgt catggaaatt cgtggccgtg cgtctgtaat atccgagatc
      181 tgccaaggct ggcttcctag ttgtcagaac aggctgaaca ggcatgttgg aaacccacca
      241 ttttgcaaaa tggagctttg tattgttagg gtgtgtaatt aagacctcat tgtgacagcc
      301 attgagaaaa agctaatgag ggttctgatg tattccatat gaatatgaat attgaatttt
      361 taatatttta tagctttcta attgccttgg tttctgttaa tggctttgac tgttgaatat
      421 tacccagact gagtgtgtgg catgcgtaag tacctttaag ctgagagctg ataaaatagc
      481 cacatgctca agccgaattc tttattttat gattgtcgtg tatgtatctt tgaggcagac
      541 acaaatctcg actgaaggca tgtgtgtgac tgtgaataca tttcaaccac tcttttactc
      601 cctcgccaag atgaatgtat atataattac aaagtatatc acaagcacta ccttgatata
      661 gagtttaatg gccgtatcgt ttctttgtgg agcatgtctg ttggccttaa tcttccctac
      721 agctgacatc ttaataactc agccctctcc atctctttcc acgggagaca tccggatgtt
      781 ggggaaggat tagtttaatg aatttgacaa ttagtaacag aagttaatga ctgcatgggg
      841 ctaagtaccc tgatggacat cgctgggggg ggactgaggg cttccattgc tctgggccat
      901 agatcctcat taatagctat tccgcctgct ggagaggaca ggagaagcca gtccgggcct
      961 ttgtttagtc tgcctctgaa ggggctgcta gggggttgcg ctgttgcaca acgccacgcc
     1021 agaattagtc ccgctctggg cgtgagttaa tgcagggcct caggtcctgg atcaggtgaa
     1081 gcttctttta atgagctagg gtcattaaac gttgaaaaaa gaaatgtgac atggaatcag
     1141 ggcgggcagg gaactctctc ttcggatctt gtgccaacag tcatgaagaa agaaaagtgg
     1201 cttgtgggtc acaagagtcc tgatgacctc tgccttgatt tatcctcatg ttagcttctt
     1261 atgtggattc cacctttaat gcagtcaaaa catcgtttag ccagatagct ctcataaaga
     1321 ttttattatt atttattatt acactgtgcc tcatgagcta ccttgtcgct ttgtcaggta
     1381 ttcccactat aaaccatttt gataaatttt tcagctagca taaaatgaaa gtttgtattt
     1441 tttatggcat catgaataca catgaaacaa ttcccataaa atccatcaat ttatattttt
     1501 acatacatat accacttctt cacaataatt catcgtacct ttcccacagt ggatgg
//
LOCUS       KCVB01000836            1177 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39706pri ultra-conserved element locus
            uce-365, sequence.
ACCESSION   KCVB01000836
VERSION     KCVB01000836.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39706pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1177
                     /note="ultra-conserved element locus uce-365"
BASE COUNT          322 a          233 c          303 g          319 t
ORIGIN      
        1 cagccccttg gcatgatcgg ttatgaatac cctgtcaaaa gtggttggta aaaggatgta
       61 aatattctat tgatgtttgc gattattgat ttgttccctg acccatcgta tcacggccaa
      121 tggcaggttt ccaaaagaat gaagctcttc ctctgagagc cactgactca cagaggctga
      181 gtaggtgggg agggagacag agaggacaag atgagggatg ttgagagagt gagggaaaca
      241 ctgtgaacgt cagactcggt gaagagaagg ctactatatg tatacagcta gagagaggag
      301 catggagcgt tgagagcgaa ggggagatcc agacgcagac atcagcggag cagagctgac
      361 ctgactggtg tgtgatcagt ggcagaatgg atctaactca gagatgatgc tgacagtgga
      421 gcttcgctgg tccttcagat gaccacagcc ggcagctgag gcccgtcatt attcaatctc
      481 acattcagaa gaatgcaaca agcaaatctc acatagcagc acagcaagat ctcagtttgt
      541 ggacacaatc attctattag gaatgccctg gcttggcctt gtgtttcttt ccagatgatg
      601 gattcaaaat gaaataatag aacaatctca ataactccag gacattcaga tggcgtgaat
      661 atgcagaatc cgagggaaag agtccatgac tgtcgactca gctggagaag catgataact
      721 aagagtgtta ctaacatata tgaattgtaa agtttttttg tttttttttt caatttctca
      781 agtcttcagt cctcttgctg cttccaatca cactctgtat taacattggt ccttaacttt
      841 aaagtctctt gtgtatgtgt gttttttcat atttttcttc ttcatgggac atgatcatcc
      901 agtgtttagt gcttctaaga aaaatagctg gtccttcaga agagtctgtg tatctctatt
      961 tcttaaatgg agctaatcta ctaaaattct gccagaaagg agcctggcct cttttggatt
     1021 gactgaattc aaaaacctct ccttgtgcca tgtcctccgt tacctcatac tgccccgcac
     1081 tgagggggtc ctgggggggc gggtatgagg ggggcgtgca cctgtttaca cctagggagg
     1141 caggtgcaaa aagagcagga agggggtgca aggggcg
//
LOCUS       KCVB01000837             991 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39748pri ultra-conserved element locus
            uce-749, sequence.
ACCESSION   KCVB01000837
VERSION     KCVB01000837.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 991)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39748pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>991
                     /note="ultra-conserved element locus uce-749"
BASE COUNT          330 a          155 c          173 g          333 t
ORIGIN      
        1 ttttggaaag aaagtaaaaa cataatgaac acaagatacg catactagtt attgttaaag
       61 aaaatagctg ccctgtaaaa atctgctcag taaaaatatt caaactgtaa tcttgtggtt
      121 gttgcttttt gttgttggtt tgaattgttt tctagatcag ctggctcatt tagctttcaa
      181 cagttagtgt atttgatcca gctggatctc agggtaaaaa gaggcgacat cttaaaagca
      241 ctgaagccag cctgcaaaga tgcagaatag agaggaaaaa aacacaaaaa cccaaactaa
      301 agcaaactca cagagttcga cagctactaa ggcaactttt gccaaataca atataagccc
      361 atgtatattg caacacaact atatattaat ttaataaaat acacaatata gcttttgtac
      421 acataacaat ttggctcaaa tgcacagaaa ttgcagtaaa atcaataaaa atatgtcatt
      481 ttttaatgta aacacatcta aattggatac atttacacat ataatgatat tatgtatagt
      541 gataatgtga agggacattt tcttgttggt ttatgttttt tggatatttg tcttcgaaac
      601 aatggcagtg tcctttgcag ttctttgtgt taacacccct ctcttgacct taactcgagg
      661 acactttttt gctgagattt ctcatttctt tttttcttat aaaatgtata aatattttgt
      721 taatatttgg acactataca tggattcata catattatca tctatatgat catatagata
      781 tcatcttttc acagttgagg aaaaatgtcc ctttttatac aacttgtgat tgccttaagg
      841 cttaagtcac acacatgata attcactgta cacaagggac tgaaagctga tgatgttcat
      901 gtataagaca ttacacagat ggccagttgt gtgtaagcgt tagtgtgttt gtgtgcgtgt
      961 gtgtagaggc atcagtgcat gtgagtgtta g
//
LOCUS       KCVB01000838            1615 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39791pri ultra-conserved element locus
            uce-1013, sequence.
ACCESSION   KCVB01000838
VERSION     KCVB01000838.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39791pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1615
                     /note="ultra-conserved element locus uce-1013"
BASE COUNT          518 a          350 c          336 g          411 t
ORIGIN      
        1 acaaaacaca tacttaagtc cactaaaact accatgtttg acattgccaa gatgggctgt
       61 gatgctataa actctcatgc tatttccaga atcttatagc acaacattta cactacagcc
      121 taaataatta catgagtcat taagcgactt tattgacctt ttgcaccttg tcaggccttt
      181 atgatctcga gcattaggtt cctataaaca ctctgtaaac agagacgcta aatgatgcac
      241 gtgtgctctc tgtaacacag tgcacaagtg caagcttgtt cacacacacg cacactctgt
      301 aacagagtga cggattatta cggacataat atgcacacac acacacccaa gttcatgctc
      361 caaaagtctg gcatccacat aaattcacac atgtcttgat tcgttcccca gcattccaca
      421 gcgctcagca accatcacct accgtacctt cagtttctcg attcccctca cgtgtctttg
      481 tatttccaga ctactaagag gctctgttgc tcctcgcttg acctctcagc attgcccttt
      541 ttaattgaaa cacagctgcc tctctctttg ccagtctgtc aatcagtcag ccaggcagtc
      601 agtccattca caagctcccc acaccagcca cggatgccca ttcacaaaaa tgaaaaaaaa
      661 agaaacaccc agggaggaat atccaatgga gagaagcaaa agcagcgcaa cagaggagag
      721 cagagtgtga ttcttgggtc tgtctgtgta gcagagtggc ttgtgtggag ttaagagtca
      781 ggctcactgt tgtgtgtggc tcatgtagga cgagcgagag agtgagagga agggagggag
      841 ggcagcagca ctgcgctgtg gagatgtcgc accactagtt tttatagcag ggtgggtagc
      901 tggaagataa taatacccca tcctcctcca caacacaagt ttaagcgcac tgtgtaataa
      961 gcccatggcg cagacaccct ctgatttagg tcagacaaaa tgaattttta gttgtgttag
     1021 agtggatttt ggtataaagg gaccggggca aagaaactcg aaggggttgt ttggggagac
     1081 taagggaggg taaaaaaaaa aaaaaaggag aaaaaaagga aatgagaaag agagagaaaa
     1141 gttgaaaact attttgtgca cctctgttgg ttagtctcaa gctgggatga agagatgtac
     1201 aggaataaaa gcagtaatac agaagcagaa acccaactgc aacttaaagc cagttagttt
     1261 cattctttac atctgtttaa aatggagtcc tgctattatc tcttatctat aatcatacta
     1321 acatttagta agttctttga aacttctgtg catgtcttca aacaagtaca gggtcaaaga
     1381 caggtgaaaa gatccacact gagcaagtta aacctcatta atattcagtc acaacagact
     1441 aattattaga aaataataac atggtgtgcg tcacactgca tttagtacgt ttcacacttt
     1501 ctgaattagt ccatggtgtc agttttaacg gaaatattgc ctaaaagcta tcaaaggcaa
     1561 gattgaacca caaacgtcaa aaaacaccac aagcaacgtc gtgcagactt atttg
//
LOCUS       KCVB01000839            1614 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39826pri ultra-conserved element locus
            uce-60, sequence.
ACCESSION   KCVB01000839
VERSION     KCVB01000839.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39826pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1614
                     /note="ultra-conserved element locus uce-60"
BASE COUNT          417 a          358 c          371 g          468 t
ORIGIN      
        1 atctcacaaa cggatttcaa gtgagagtgt gttttaatgg ttaattgcga taaggaaacg
       61 cgtttcctct gattcaaata actgacctaa aagccaaatg cgcacgtcgg ctgccacagt
      121 tgttccgggc tcacagtgtg taaaatgtga acaagaagcc tgcgagcatc gctgaggctt
      181 tggctgattg agagaaacag acctgtgcgc acactttgac cagagtattg ttctcaagtg
      241 tccccctgac cggctcacgg gcaaatccga aagtaaaggg ctaaaggtaa aggagcatgt
      301 tgagaataat gacagatggg aaacttgcgt catgccagga cagcgcgttt ttctagtcga
      361 atataaatgg acaacaaaac cgctggcagc caaaactgag cccccttaac atgagggact
      421 cgtttcactt catctcgtac gctggcgtca catttattga atgtctgaga tcctctaaag
      481 gatgaagttt ccgataatgt agagcttatt atttggttgg taaagccagc ataactacat
      541 ggaagtcaga ggtattccat gcatatcacc ctgaagtcaa cgagcatgtt taaatctctt
      601 gtataggaaa ttcctcttat ttctcccctg tgataatagc gtaatagata ttgtatagac
      661 ccatcccttt tcctactttt ctcaatttct tacctgcact tcgaagctcg ttcaaaaagt
      721 ggcgacagcg tttccacggc tccccctgtt ttcccccgat gaagcgcgta tagttttttt
      781 tttttaatat gttcctttac tttgtttcat ttctcacttc catcttcttt tcatctcatc
      841 aataaacaca cgctgcagcc taccttcata gagcagctcg gttcaagtgt gccctcccat
      901 tttttaacta tacatcgagt actttgaggg gtggtggtgg tggtggtggt ggtgaagggg
      961 agaggggggg gacaggcagg cacgcctttt cacttaaagc agctattgga agaacagtgc
     1021 actgtcttgg ggttggatca gaaatccgtc aacattattg tgccccaatc gcagggagca
     1081 cggtgggact gtgaaaccgg ctccattatg gctttctgga agtggtggga gttatagtaa
     1141 tagtagaata gtagttgtag tagtagtgat cctcctactc gaggtattta agtttgctcc
     1201 tgcctcagca gcaacacagc aattttataa acgaaagtca tggtgtgtaa cgaataaagg
     1261 atgagagctg aaggaggttc gcagcgagtg ttcactttac cctttgatcc caccttattt
     1321 atttaatttg tatgtatata ttttataact gtgtgggctc caacgtctgc tgttcaaaat
     1381 atcagcgtcc acaattgtga atgaactcaa atctatcccc agtctggatc aaagggacgg
     1441 gggaattgtc agttatgtgt ccatcatgcg actgaacttt gcattggacg ccgtctctgc
     1501 cagtacagta atccagctgc gccgcggcct ctcattggac agcggcgccg tcaatcatat
     1561 ccagcaccaa caagctagat tgtctgccgt gtcttctact ttctacgcat ttct
//
LOCUS       KCVB01000840            1557 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39912pri ultra-conserved element locus
            uce-862, sequence.
ACCESSION   KCVB01000840
VERSION     KCVB01000840.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39912pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1557
                     /note="ultra-conserved element locus uce-862"
BASE COUNT          482 a          319 c          299 g          457 t
ORIGIN      
        1 gtgacaaggc caaggcacgt gcctcccatt gttgagccgt atcctccagg gtcagtcgca
       61 cacccagtca gaggtcagat gtcacccagg cctctctttt gggtcaccac tcagcaacct
      121 tcagcctttg acctcaacaa acactgaaac aaattgggag atgggggaga gaggggagag
      181 agaggacgaa attaaacata tttactcatt gatgctccat gaccacatga cacttttgga
      241 tattctgaca aactcaaact gcatccgtag ttttaagtgc agataagcag tgcagtcttc
      301 tgtgggcaat gtacagtgtg ttggagaatc actctgcatg ttacagcatg tctccctctt
      361 gtggtgaaat gtaatgcttt gaaaatgtta cattttaaag tgtggcacaa aaatacacaa
      421 tacagggagt cctggtggtt aagactgaca ctatgtaacc acgacgacct tccaagtcac
      481 ctccctttct ttcctgatgt ttcttgtcca aatctataac gttaactata tgtattaaag
      541 gataaaacac caaaaaatgc actgtgtgtc atatttatgc tgagatttgt tatactggtg
      601 tgtcaaaaac atgagataca tatgactttt tataagtgag gtttttttca gacagtcact
      661 tcatgctgga ttcaaataaa acatataatt ttacatctca tacatttaaa gattcattgt
      721 ggtcatgcat acatcctgct gaaaaagcct cttgacccaa ccttctcatg tgtaattacc
      781 taaaaacact gtcagctatc gcagtttaac tgaccaaatc agacatatgt ccacacattt
      841 cggagagtct ttcgccagag gtgccatctt ttccattgag cctcgatgca tgttgggata
      901 ttcaggtcat cgagttgcaa agtgttgctc aggcgttgac aaaagaaaaa tcttaagttt
      961 attaaacgga acaggctgac agtccacact ttgttaggca gtgaatgaac agagtggttt
     1021 tgacaggcct aacctcttaa gcttcgggtc ccactgagct cggcacagct agagctaaat
     1081 gccatctgtc ccaacatcgc aaggcccccc caacctgtcc cccaacagtt tagcataaaa
     1141 atcatacaat actgctatta aggaagaata gtagataatt ctgctaatgt gatatcaagt
     1201 tcctaaatta aaaataacat cgttagtgaa ggatatatca tttgaaaacg acagcaatgc
     1261 ttgtaattta cttttctttt ttttttgaag gaaacaggca caataaatta tcagcaaacc
     1321 agctttctta tcaagactaa taaaagttgc tgtgaatgtt ttttttttat ttaacaagtt
     1381 cagtgactta agaaaaactg aactctcttc tgttgtctga ctctttctgc ctacacgtgt
     1441 tgtctgttgc cttgcgcgcc tactggcact gacagggaat tgagaaaaga cttgagaagg
     1501 aagaaattaa tagtgttggt gctttattat aactagaaaa aaaaacaaaa taatata
//
LOCUS       KCVB01000841            1436 bp    DNA     linear   VRT 21-NOV-2019
DEFINITION  TLS: Prionurus punctatus 39952pri ultra-conserved element locus
            uce-25, sequence.
ACCESSION   KCVB01000841
VERSION     KCVB01000841.1
DBLINK      BioProject: PRJNA516931
            BioSample: SAMN10885231
KEYWORDS    TLS; Targeted Locus Study.
SOURCE      Prionurus punctatus (yellowtail surgeonfish)
  ORGANISM  Prionurus punctatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Acanthuriformes; Acanthuridae;
            Prionurus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Bernal,M.A., Salas,E., Kenworthy,E. and Chakrabarty,P.
  TITLE     Genomic, ecological, and morphological approaches to investigating
            species limits: a case study in modern taxonomy from Tropical
            Eastern Pacific surgeonfishes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Ludt,W.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Vertebrate Zoology; Division of Fishes,
            National Museum of Natural History, Smithsonian Institution, 10th
            and Constitution Ave, NW, Washington, DC 20560-0159, United States
            of America
COMMENT     We identified several hundred ultra-conserved elements (UCEs) in 3
            species to examine the evolutionary relationships within Eastern
            Pacific Prionurus surgeonfishes.  All of the UCEs from one fish are
            included in a single TLS project.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity ver 2.0.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Prionurus punctatus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="39952pri"
                     /specimen_voucher="LSUMZ:Ichthyology:6614"
                     /db_xref="taxon:1316016"
                     /tissue_type="muscle"
                     /dev_stage="Adult"
                     /country="Costa Rica"
     misc_feature    <1..>1436
                     /note="ultra-conserved element locus uce-25"
BASE COUNT          389 a          356 c          263 g          428 t
ORIGIN      
        1 cacgcacaca catttacact catgaacaca caaagccccc ccatcccctt gagactttgg
       61 cccacccctc tgctcattgc ccagctgtat cactttgcct ctggacaatt cccacttgcc
      121 gcgtcagggg gcctaatggg ccgctgctat tcagcagcag attaaatatt aatatcgcca
      181 cgatacagag attgcagcgc atctccagtt acaatagaga tacacagatc aaagtgccga
      241 ggcaatagtg ggtgcatgaa agcatttaaa gagtgaaaac tgcaactaaa cagtacgagg
      301 gaagctcttt aaaacatcaa actatagtga gccgagaaga tgaagaactt ttttctcttt
      361 tcacaagaat tgagtatgaa cacgtttaca aagccggttt ttagtaatag tgtgcactca
      421 ttagccctgg ctccaaagag gaccctcccc cgcttctacc tcccctctcg gtgctacatt
      481 tgcccttttt atgagccctc cttttccatt ttttttcctc cctcctcttc tatcctttct
      541 ctttttttcc caaccccaac acggaaattg aaactgtatc tgggcttatc tgcagcctcc
      601 ccgtgcatca actgccgaga tgtttagaca aattttatgc attatgcagc gcaaatatca
      661 aaacagttct tggcagtgcc agcagaagcc tcattaaaaa ttccttaatt aaatcttctt
      721 gcaaaatgta atcagtccaa ggaaggggtt ttgtgtgtgt gtgtatgtgt gtgcagagga
      781 ggacgcgtgt gtatgtgtgc gcgtctgtca gtgccaaggc tttgatttgt gcccttcatg
      841 ttatggcttg gagaagtaat cattaacgcc aaagccactt tggcttttca aatgctgttt
      901 ttctctcttt ttaaatggaa taaacaaaca agtgataatc acctttctag tgtcggctca
      961 aaatgaatcc agaatggggg acattgttct gtgtccattg aaacgcaaga tacctcccct
     1021 cccctgatgt ttttgtcttt gtcggtgtga atgactgact ttttacacca gtcagttcct
     1081 cgtgtgttca acaataaacg gcagaactca ggtagaacct gacctgagct catatcctct
     1141 ctgtgtcagc taacagctaa caggacagat ttaaacagaa ttatgttctc tatgttttag
     1201 tgttatacac accatgagta ttcattcagc cagttacagt aaactaccat caaaccattt
     1261 cactttcatt acccctcatc cttctacttc ccctcagcac ataaattgca gtcttttctt
     1321 aatcaattta ctgcacacag aacattctca caccagtcaa cgatgtagtt acatcactga
     1381 ataaaacaca ctatcagagg acggaaaagg cttcggctct gttttgggta tatttt
//
